MX377162B - Nuevas enzimas y sistemas crispr - Google Patents

Nuevas enzimas y sistemas crispr

Info

Publication number
MX377162B
MX377162B MX2017016688A MX2017016688A MX377162B MX 377162 B MX377162 B MX 377162B MX 2017016688 A MX2017016688 A MX 2017016688A MX 2017016688 A MX2017016688 A MX 2017016688A MX 377162 B MX377162 B MX 377162B
Authority
MX
Mexico
Prior art keywords
methods
systems
targeting
crispr systems
new enzymes
Prior art date
Application number
MX2017016688A
Other languages
English (en)
Other versions
MX2017016688A (es
Inventor
Bernd Zetsche
Feng Zhang
Ian Slaymaker
Jonathan S Gootenberg
Omar O Abudayyeh
Original Assignee
Broad Inst Inc
Massachusetts Inst Technology
Harvard College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=55274964&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX377162(B) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Broad Inst Inc, Massachusetts Inst Technology, Harvard College filed Critical Broad Inst Inc
Publication of MX2017016688A publication Critical patent/MX2017016688A/es
Publication of MX377162B publication Critical patent/MX377162B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P21/00—Drugs for disorders of the muscular or neuromuscular system
    • A61P21/04—Drugs for disorders of the muscular or neuromuscular system for myasthenia gravis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P27/00—Drugs for disorders of the senses
    • A61P27/02—Ophthalmic agents
    • A61P27/06—Antiglaucoma agents or miotics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3519—Fusion with another nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Ophthalmology & Optometry (AREA)
  • Epidemiology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Mycology (AREA)
  • Neurology (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Physical Education & Sports Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

La presente invención se refiere a sistemas, métodos y composiciones para el direccionamiento de ácidos nucleicos. En particular, la invención provee sistemas de direccionamiento de adn no natural o manipulado que comprende una nueva proteína efectora crispr de direccionamiento de adn o arn y por lo menos un componente de ácido nucleico de direccionamiento tal como un arn guía. También se divulgan y reivindican métodos para elaborar y utilizar, y los usos, de dichos sistemas, métodos y composiciones, y productos de dichos métodos y usos.
MX2017016688A 2015-06-18 2016-06-17 Nuevas enzimas y sistemas crispr MX377162B (es)

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US201562181739P 2015-06-18 2015-06-18
US201562193507P 2015-07-16 2015-07-16
US201562201542P 2015-08-05 2015-08-05
US201562205733P 2015-08-16 2015-08-16
US201562232067P 2015-09-24 2015-09-24
US14/975,085 US9790490B2 (en) 2015-06-18 2015-12-18 CRISPR enzymes and systems
EP16150428.7A EP3009511B2 (en) 2015-06-18 2016-01-07 Novel crispr enzymes and systems
PCT/US2016/038181 WO2016205711A1 (en) 2015-06-18 2016-06-17 Novel crispr enzymes and systems

Publications (2)

Publication Number Publication Date
MX2017016688A MX2017016688A (es) 2018-12-11
MX377162B true MX377162B (es) 2025-03-07

Family

ID=55274964

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2017016688A MX377162B (es) 2015-06-18 2016-06-17 Nuevas enzimas y sistemas crispr
MX2020011939A MX2020011939A (es) 2015-06-18 2017-12-18 Nuevas enzimas y sistemas crispr.

Family Applications After (1)

Application Number Title Priority Date Filing Date
MX2020011939A MX2020011939A (es) 2015-06-18 2017-12-18 Nuevas enzimas y sistemas crispr.

Country Status (16)

Country Link
US (2) US9790490B2 (es)
EP (6) EP3009511B2 (es)
JP (4) JP6898865B2 (es)
KR (2) KR20230170151A (es)
CN (6) CN115572737A (es)
AU (3) AU2016278990B2 (es)
CA (1) CA2989834A1 (es)
DK (5) DK3009511T3 (es)
ES (4) ES2646140T3 (es)
FI (2) FI3604532T3 (es)
IL (3) IL290678B2 (es)
MX (2) MX377162B (es)
RU (1) RU2737537C2 (es)
SG (1) SG10201912330QA (es)
TW (2) TWI837592B (es)
WO (1) WO2016205711A1 (es)

Families Citing this family (823)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3613852A3 (en) 2011-07-22 2020-04-22 President and Fellows of Harvard College Evaluation and improvement of nuclease cleavage specificity
US10704021B2 (en) 2012-03-15 2020-07-07 Flodesign Sonics, Inc. Acoustic perfusion devices
US9458450B2 (en) 2012-03-15 2016-10-04 Flodesign Sonics, Inc. Acoustophoretic separation technology using multi-dimensional standing waves
WO2013163628A2 (en) 2012-04-27 2013-10-31 Duke University Genetic correction of mutated genes
KR102271292B1 (ko) 2013-03-15 2021-07-02 더 제너럴 하스피탈 코포레이션 Rna-안내 게놈 편집의 특이성을 증가시키기 위한 rna-안내 foki 뉴클레아제(rfn)의 용도
US9828582B2 (en) 2013-03-19 2017-11-28 Duke University Compositions and methods for the induction and tuning of gene expression
MX369747B (es) 2013-04-16 2019-11-20 Regeneron Pharma Modificación dirigida del genoma de rata.
BR122021009076B1 (pt) * 2013-06-17 2024-02-15 The Broad Institute Inc. Vetor viral contendo molécula(s) de ácido nucleico heterólogo, composição, uso e métodos do mesmo
US20150044192A1 (en) 2013-08-09 2015-02-12 President And Fellows Of Harvard College Methods for identifying a target site of a cas9 nuclease
US9359599B2 (en) 2013-08-22 2016-06-07 President And Fellows Of Harvard College Engineered transcription activator-like effector (TALE) domains and uses thereof
US9388430B2 (en) 2013-09-06 2016-07-12 President And Fellows Of Harvard College Cas9-recombinase fusion proteins and uses thereof
US9737604B2 (en) 2013-09-06 2017-08-22 President And Fellows Of Harvard College Use of cationic lipids to deliver CAS9
US9228207B2 (en) 2013-09-06 2016-01-05 President And Fellows Of Harvard College Switchable gRNAs comprising aptamers
CN111218447B (zh) 2013-11-07 2024-10-11 爱迪塔斯医药有限公司 使用统治型gRNA的CRISPR相关方法和组合物
RU2685914C1 (ru) 2013-12-11 2019-04-23 Регенерон Фармасьютикалс, Инк. Способы и композиции для направленной модификации генома
US11053481B2 (en) 2013-12-12 2021-07-06 President And Fellows Of Harvard College Fusions of Cas9 domains and nucleic acid-editing domains
CN105939767B (zh) 2014-01-08 2018-04-06 弗洛设计声能学公司 具有双声电泳腔的声电泳装置
HUE066611T2 (hu) 2014-02-11 2024-08-28 Univ Colorado Regents CRISPR-aktivált multiplex genommérnökség
EP3154694B1 (en) 2014-06-13 2025-01-29 Children's Medical Center Corporation Products and methods to isolate mitochondria
WO2015200805A2 (en) 2014-06-26 2015-12-30 Regeneron Pharmaceuticals, Inc. Methods and compositions for targeted genetic modifications and methods of use
AU2015298571B2 (en) 2014-07-30 2020-09-03 President And Fellows Of Harvard College Cas9 proteins including ligand-dependent inteins
US20180250424A1 (en) 2014-10-10 2018-09-06 Editas Medicine, Inc. Compositions and methods for promoting homology directed repair
PT3207124T (pt) 2014-10-15 2019-08-27 Regeneron Pharma Métodos e composições para a produção ou conservação de células pluripotentes
EP3215617B1 (en) 2014-11-07 2024-05-08 Editas Medicine, Inc. Systems for improving crispr/cas-mediated genome-editing
RU2734770C2 (ru) 2014-11-21 2020-10-23 Регенерон Фармасьютикалз, Инк. Способы и композиции для нацеленной генетической модификации с использованием парных гидовых рнк
KR102656470B1 (ko) 2014-12-10 2024-04-09 리전츠 오브 더 유니버스티 오브 미네소타 질환을 치료하기 위한 유전적으로 변형된 세포, 조직 및 장기
RU2707137C2 (ru) 2014-12-19 2019-11-22 Регенерон Фармасьютикалз, Инк. Способы и композиции для нацеленной генетической модификации посредством одноэтапного множественного нацеливания
WO2016130600A2 (en) 2015-02-09 2016-08-18 Duke University Compositions and methods for epigenome editing
WO2016161207A1 (en) * 2015-03-31 2016-10-06 Exeligen Scientific, Inc. Cas 9 retroviral integrase and cas 9 recombinase systems for targeted incorporation of a dna sequence into a genome of a cell or organism
GB201506509D0 (en) * 2015-04-16 2015-06-03 Univ Wageningen Nuclease-mediated genome editing
US11708572B2 (en) 2015-04-29 2023-07-25 Flodesign Sonics, Inc. Acoustic cell separation techniques and processes
US11377651B2 (en) 2016-10-19 2022-07-05 Flodesign Sonics, Inc. Cell therapy processes utilizing acoustophoresis
EP3294896A1 (en) 2015-05-11 2018-03-21 Editas Medicine, Inc. Optimized crispr/cas9 systems and methods for gene editing in stem cells
US11535871B2 (en) * 2015-05-14 2022-12-27 University Of Southern California Optimized gene editing utilizing a recombinant endonuclease system
JP7396783B2 (ja) 2015-06-09 2023-12-12 エディタス・メディシン、インコーポレイテッド 移植を改善するためのｃｒｉｓｐｒ／ｃａｓ関連方法および組成物
CA3012607A1 (en) * 2015-06-18 2016-12-22 The Broad Institute Inc. Crispr enzymes and systems
US9790490B2 (en) * 2015-06-18 2017-10-17 The Broad Institute Inc. CRISPR enzymes and systems
CA3012631A1 (en) * 2015-06-18 2016-12-22 The Broad Institute Inc. Novel crispr enzymes and systems
US10648020B2 (en) 2015-06-18 2020-05-12 The Broad Institute, Inc. CRISPR enzymes and systems
EP4545544A3 (en) 2015-06-29 2025-10-08 Ionis Pharmaceuticals, Inc. Modified crispr rna and modified single crispr rna and uses thereof
WO2017015015A1 (en) * 2015-07-17 2017-01-26 Emory University Crispr-associated protein from francisella and uses related thereto
US11474085B2 (en) 2015-07-28 2022-10-18 Flodesign Sonics, Inc. Expanded bed affinity selection
US11459540B2 (en) 2015-07-28 2022-10-04 Flodesign Sonics, Inc. Expanded bed affinity selection
IL257105B (en) 2015-07-31 2022-09-01 Univ Minnesota Modified cells and methods of therapy
AU2016308339A1 (en) 2015-08-18 2018-04-12 Baylor College Of Medicine Methods and compositions for altering function and structure of chromatin loops and/or domains
US11530424B1 (en) * 2015-09-18 2022-12-20 University Of South Florida Efficient CRISPR/HDR-mediated knock-in system and method of use
CA2998287A1 (en) * 2015-09-24 2017-04-20 Crispr Therapeutics Ag Novel family of rna-programmable endonucleases and their uses in genome editing and other applications
WO2017053879A1 (en) 2015-09-24 2017-03-30 Editas Medicine, Inc. Use of exonucleases to improve crispr/cas-mediated genome editing
CA3001008A1 (en) 2015-10-05 2017-04-13 Precision Biosciences, Inc. Engineered meganucleases with recognition sequences found in the human t cell receptor alpha constant region gene
CN108601821B (zh) 2015-10-05 2023-09-19 精密生物科学公司 包含经修饰的人T细胞受体α恒定区基因的经遗传修饰的细胞
MX2018004263A (es) 2015-10-08 2019-08-16 Harvard College Edición genómica multiplexada.
US11692182B2 (en) 2015-10-09 2023-07-04 Monsanto Technology Llc RNA-guided DNA nucleases and uses thereof
WO2017066497A2 (en) 2015-10-13 2017-04-20 Duke University Genome engineering with type i crispr systems in eukaryotic cells
JP2018531261A (ja) * 2015-10-16 2018-10-25 テンプル ユニバーシティー オブ ザ コモンウェルス システム オブ ハイヤー エデュケーション Ｃｐｆ１を用いた、ｒｎａガイド遺伝子編集方法および組成物
US12043852B2 (en) 2015-10-23 2024-07-23 President And Fellows Of Harvard College Evolved Cas9 proteins for gene editing
WO2017070598A1 (en) 2015-10-23 2017-04-27 Caribou Biosciences, Inc. Engineered crispr class 2 cross-type nucleic-acid targeting nucleic acids
EP3368054A4 (en) 2015-10-28 2019-07-03 Voyager Therapeutics, Inc. REGULATORY EXPRESSION USING THE ADENO-ASSOCIATED VIRUS (AAV)
CA3004497A1 (en) * 2015-11-06 2017-05-11 The Jackson Laboratory Large genomic dna knock-in and uses thereof
US11905521B2 (en) * 2015-11-17 2024-02-20 The Chinese University Of Hong Kong Methods and systems for targeted gene manipulation
EP3384055B1 (en) 2015-11-30 2025-07-16 Duke University Therapeutic targets for the correction of the human dystrophin gene by gene editing and methods of use
WO2017095946A1 (en) 2015-11-30 2017-06-08 Flagship Pioneering, Inc. Methods and compositions relating to chondrisomes
US9988624B2 (en) 2015-12-07 2018-06-05 Zymergen Inc. Microbial strain improvement by a HTP genomic engineering platform
US11293029B2 (en) 2015-12-07 2022-04-05 Zymergen Inc. Promoters from Corynebacterium glutamicum
US11208649B2 (en) 2015-12-07 2021-12-28 Zymergen Inc. HTP genomic engineering platform
WO2017099494A1 (ko) * 2015-12-08 2017-06-15 기초과학연구원 Cpf1을 포함하는 유전체 교정용 조성물 및 그 용도
US12110490B2 (en) 2015-12-18 2024-10-08 The Broad Institute, Inc. CRISPR enzymes and systems
EP4159848A1 (en) 2015-12-29 2023-04-05 Monsanto Technology LLC Novel crispr-associated transposases and uses thereof
US11427837B2 (en) * 2016-01-12 2022-08-30 The Regents Of The University Of California Compositions and methods for enhanced genome editing
EP3402490B1 (en) 2016-01-15 2022-06-01 The Children's Medical Center Corporation Therapeutic use of mitochondria and combined mitochondrial agents
US20190264186A1 (en) * 2016-01-22 2019-08-29 The Broad Institute Inc. Crystal structure of crispr cpf1
US9896696B2 (en) 2016-02-15 2018-02-20 Benson Hill Biosystems, Inc. Compositions and methods for modifying genomes
WO2017151719A1 (en) * 2016-03-01 2017-09-08 University Of Florida Research Foundation, Incorporated Molecular cell diary system
KR20240173370A (ko) * 2016-03-04 2024-12-11 에디타스 메디신, 인코포레이티드 암 면역요법을 위한 crispr-cpf1-관련 방법, 조성물 및 구성성분
WO2017165826A1 (en) 2016-03-25 2017-09-28 Editas Medicine, Inc. Genome editing systems comprising repair-modulating enzyme molecules and methods of their use
US20190127713A1 (en) 2016-04-13 2019-05-02 Duke University Crispr/cas9-based repressors for silencing gene targets in vivo and methods of use
US11236313B2 (en) 2016-04-13 2022-02-01 Editas Medicine, Inc. Cas9 fusion molecules, gene editing systems, and methods of use thereof
KR102424476B1 (ko) 2016-04-19 2022-07-25 더 브로드 인스티튜트, 인코퍼레이티드 신규한 crispr 효소 및 시스템
WO2017189308A1 (en) 2016-04-19 2017-11-02 The Broad Institute Inc. Novel crispr enzymes and systems
EP3445853A1 (en) 2016-04-19 2019-02-27 The Broad Institute, Inc. Cpf1 complexes with reduced indel activity
JP2019514379A (ja) * 2016-04-28 2019-06-06 インダストリ−アカデミック コオペレーション ファウンデイション， ヨンセイ ユニヴァーシティＩｎｄｕｓｔｒｙ−Ａｃａｄｅｍｉｃ Ｃｏｏｐｅｒａｔｉｏｎ Ｆｏｕｎｄａｔｉｏｎ， Ｙｏｎｓｅｉ Ｕｎｉｖｅｒｓｉｔｙ Ｒｎａ誘導型ヌクレアーゼ活性のインビボ高スループット評価のための方法
US11085035B2 (en) 2016-05-03 2021-08-10 Flodesign Sonics, Inc. Therapeutic cell washing, concentration, and separation utilizing acoustophoresis
US11214789B2 (en) 2016-05-03 2022-01-04 Flodesign Sonics, Inc. Concentration and washing of particles with acoustics
US11499158B2 (en) 2016-05-13 2022-11-15 Kaneka Corporation Method for modifying plant
WO2017195905A1 (ja) 2016-05-13 2017-11-16 株式会社カネカ 形質転換植物の作製方法
WO2017195906A1 (ja) * 2016-05-13 2017-11-16 株式会社カネカ 植物のゲノム編集方法
EP4368637A3 (en) 2016-05-20 2024-07-10 Regeneron Pharmaceuticals, Inc. Methods for breaking immunological tolerance using multiple guide rnas
CA3025828A1 (en) 2016-06-01 2017-12-07 Kws Saat Se Hybrid nucleic acid sequences for genome engineering
WO2017210380A1 (en) * 2016-06-01 2017-12-07 Excision Biotherapeutics, Inc. Compositions and methods of treatment for lytic and lysogenic viruses
CN109983124B (zh) * 2016-06-02 2022-09-16 西格马-奥尔德里奇有限责任公司 使用可编程dna结合蛋白增强靶向基因组修饰
US20200332305A1 (en) 2016-06-14 2020-10-22 Pioneer Hi-Bred International, Inc. Use of cpfi endonuclease for plant genome modifications
US10337051B2 (en) * 2016-06-16 2019-07-02 The Regents Of The University Of California Methods and compositions for detecting a target RNA
CA3028158A1 (en) * 2016-06-17 2017-12-21 The Broad Institute, Inc. Type vi crispr orthologs and systems
US11293021B1 (en) 2016-06-23 2022-04-05 Inscripta, Inc. Automated cell processing methods, modules, instruments, and systems
AU2017280353B2 (en) * 2016-06-24 2021-11-11 Inscripta, Inc. Methods for generating barcoded combinatorial libraries
WO2018005655A2 (en) 2016-06-30 2018-01-04 Zymergen Inc. Methods for generating a bacterial hemoglobin library and uses thereof
EP3478845A4 (en) 2016-06-30 2019-07-31 Zymergen, Inc. METHOD OF GENERATING A GLUCOSE PERMEASE LIBRARY AND USES THEREOF
CN106191057B (zh) * 2016-07-06 2018-12-25 中山大学 一种用于敲除人CYP2E1基因的sgRNA序列、CYP2E1基因缺失细胞株的构建方法及其应用
WO2018013720A1 (en) * 2016-07-12 2018-01-18 Washington University Incorporation of internal polya-encoded poly-lysine sequence tags and their variations for the tunable control of protein synthesis in bacterial and eukaryotic cells
US11674158B2 (en) * 2016-07-15 2023-06-13 Salk Institute For Biological Studies Methods and compositions for genome editing in non-dividing cells
EP3487523B1 (en) * 2016-07-19 2023-09-06 Duke University Therapeutic applications of cpf1-based genome editing
CN109844103A (zh) 2016-07-21 2019-06-04 美克斯细胞有限公司 用于修饰基因组dna的方法和组合物
AU2017302551B2 (en) * 2016-07-26 2023-04-27 The General Hospital Corporation Variants of CRISPR from Prevotella and Francisella 1 (Cpf1)
US11078481B1 (en) 2016-08-03 2021-08-03 KSQ Therapeutics, Inc. Methods for screening for cancer targets
GB2568182A (en) 2016-08-03 2019-05-08 Harvard College Adenosine nucleobase editors and uses thereof
WO2018031683A1 (en) 2016-08-09 2018-02-15 President And Fellows Of Harvard College Programmable cas9-recombinase fusion proteins and uses thereof
TWI791459B (zh) * 2016-08-10 2023-02-11 日商領先基因生技股份有限公司 改變真核細胞基因體的標的部位之方法
EP3500671B1 (en) * 2016-08-17 2024-07-10 The Broad Institute, Inc. Method of selecting target sequences for the design of guide rnas
US20200283743A1 (en) * 2016-08-17 2020-09-10 The Broad Institute, Inc. Novel crispr enzymes and systems
US11542509B2 (en) 2016-08-24 2023-01-03 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
US11078483B1 (en) 2016-09-02 2021-08-03 KSQ Therapeutics, Inc. Methods for measuring and improving CRISPR reagent function
IL247752A0 (en) * 2016-09-11 2016-11-30 Yeda Res & Dev Compositions and methods for modulating gene expression for site-directed mutagenesis
US10650621B1 (en) 2016-09-13 2020-05-12 Iocurrents, Inc. Interfacing with a vehicular controller area network
US20190225974A1 (en) 2016-09-23 2019-07-25 BASF Agricultural Solutions Seed US LLC Targeted genome optimization in plants
WO2018064371A1 (en) * 2016-09-30 2018-04-05 The Regents Of The University Of California Rna-guided nucleic acid modifying enzymes and methods of use thereof
KR102812752B1 (ko) * 2016-09-30 2025-05-26 더 리젠츠 오브 더 유니버시티 오브 캘리포니아 Rna-가이드된 핵산 변형 효소 및 이의 사용 방법
KR20190062505A (ko) 2016-10-03 2019-06-05 주노 쎄러퓨티크스 인코퍼레이티드 Hpv-특이적 결합 분자
EP3757120B8 (en) 2016-10-04 2022-06-15 Precision Biosciences, Inc. Co-stimulatory domains for use in genetically-modified cells
KR102606680B1 (ko) 2016-10-07 2023-11-27 인티그레이티드 디엔에이 테크놀로지스 아이엔씨. Ｓ. 피오게네스 ｃａｓ９ 돌연변이 유전자 및 이에 의해 암호화되는 폴리펩티드
US11242542B2 (en) 2016-10-07 2022-02-08 Integrated Dna Technologies, Inc. S. pyogenes Cas9 mutant genes and polypeptides encoded by same
WO2018071672A1 (en) * 2016-10-12 2018-04-19 The Regents Of The University Of Colorado Novel engineered and chimeric nucleases
KR102662249B1 (ko) * 2016-10-14 2024-05-03 더 제너럴 하스피탈 코포레이션 후성적으로 조절되는 부위-특이적 뉴클레아제
GB2573062A (en) 2016-10-14 2019-10-23 Harvard College AAV delivery of nucleobase editors
JP7181862B2 (ja) 2016-10-18 2022-12-01 リージェンツ オブ ザ ユニバーシティ オブ ミネソタ 腫瘍浸潤リンパ球および治療の方法
WO2018085693A1 (en) 2016-11-04 2018-05-11 Inari Agriculture, Inc. Novel plant cells, plants, and seeds
CN106591335A (zh) * 2016-11-08 2017-04-26 安徽省农业科学院水稻研究所 一种密码子植物化改造的LbCpf1基因及其应用
JP2019535287A (ja) * 2016-11-22 2019-12-12 インテグレイテツド・デイー・エヌ・エイ・テクノロジーズ・インコーポレイテツド Ｃｒｉｓｐｒ／ｃｐｆ１システム及び方法
AU2017364106A1 (en) * 2016-11-28 2019-06-20 The Board Of Regents Of The University Of Texas System Prevention of muscular dystrophy by CRISPR/Cpfl-mediated gene editing
CN108130342B (zh) * 2016-12-01 2022-01-11 中国科学院分子植物科学卓越创新中心 基于Cpf1的植物基因组定点编辑方法
US12404514B2 (en) * 2016-12-09 2025-09-02 The Broad Institute, Inc. CRISPR-systems for modifying a trait of interest in a plant
US11293022B2 (en) 2016-12-12 2022-04-05 Integrated Dna Technologies, Inc. Genome editing enhancement
WO2018112282A1 (en) 2016-12-14 2018-06-21 Ligandal, Inc. Compositions and methods for nucleic acid and/or protein payload delivery
EP3555631A4 (en) * 2016-12-19 2020-08-12 The UAB Research Foundation MASS SPECTROMETRY STANDARDS FOR HEMOGLOBIN BETA AND HEMOGLOBIN BETA SICKLE CELL DISEASE AND THEIR USES
WO2018119010A1 (en) 2016-12-19 2018-06-28 Editas Medicine, Inc. Assessing nuclease cleavage
KR102677877B1 (ko) * 2016-12-22 2024-06-25 키진 엔.브이. 이중나선 dna의 표적화된 변형 방법
KR102151065B1 (ko) * 2016-12-23 2020-09-02 기초과학연구원 동물 배아의 염기 교정용 조성물 및 염기 교정 방법
KR102569848B1 (ko) * 2016-12-23 2023-08-25 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 Pcsk9의 유전자 편집
WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
AU2017388379A1 (en) * 2016-12-28 2019-06-06 Ionis Pharmaceuticals, Inc. Modified CRISPR RNA and uses thereof
US11859219B1 (en) 2016-12-30 2024-01-02 Flagship Pioneering Innovations V, Inc. Methods of altering a target nucleotide sequence with an RNA-guided nuclease and a single guide RNA
US12110545B2 (en) 2017-01-06 2024-10-08 Editas Medicine, Inc. Methods of assessing nuclease cleavage
US11873496B2 (en) 2017-01-09 2024-01-16 Whitehead Institute For Biomedical Research Methods of altering gene expression by perturbing transcription factor multimers that structure regulatory loops
GB201700380D0 (en) * 2017-01-10 2017-02-22 Plant Bioscience Ltd Methods of increasing seed yield
US20180201921A1 (en) * 2017-01-18 2018-07-19 Excision Biotherapeutics, Inc. CRISPRs
CA3051585A1 (en) 2017-01-28 2018-08-02 Inari Agriculture, Inc. Novel plant cells, plants, and seeds
EA201991809A1 (ru) 2017-01-30 2020-02-05 КВС ЗААТ СЕ & КО. КГаА Сцепление матрицы для репарации с эндонуклеазами для геномной инженерии
KR20190116282A (ko) * 2017-02-10 2019-10-14 지머젠 인코포레이티드 복수의 숙주를 위한 다중 dna 구조체의 조립 및 편집을 위한 모듈식 범용 플라스미드 디자인 전략
CA3053092A1 (en) * 2017-02-10 2018-08-16 National Agriculture And Food Research Organization Method for producing hsl protein having improved catalytic activity for 2-oxoglutaric acid-dependently oxidizing 4-hppd inhibitor
JP7688478B2 (ja) 2017-02-15 2025-06-04 キージーン ナムローゼ フェンノートシャップ 植物細胞における標的遺伝子変化の方法
CN106868031A (zh) * 2017-02-24 2017-06-20 北京大学 一种基于分级组装的多个sgRNA串联并行表达的克隆方法及应用
CN106947779B (zh) * 2017-02-28 2021-02-26 中国农业大学 利用乳腺生物反应器提高乳汁中褪黑素含量的方法
US10687520B2 (en) 2017-03-07 2020-06-23 The Board Of Regents Of The University Of Texas System Generation and correction of a humanized mouse model with a deletion of dystrophin exon 44
US11898179B2 (en) 2017-03-09 2024-02-13 President And Fellows Of Harvard College Suppression of pain by gene editing
WO2018165631A1 (en) 2017-03-09 2018-09-13 President And Fellows Of Harvard College Cancer vaccine
CN110914310A (zh) 2017-03-10 2020-03-24 哈佛大学的校长及成员们 胞嘧啶至鸟嘌呤碱基编辑器
EP3596207B1 (en) * 2017-03-15 2023-12-20 The Broad Institute, Inc. Novel cas13b orthologues crispr enzymes and systems
CN106978428A (zh) * 2017-03-15 2017-07-25 上海吐露港生物科技有限公司 一种Cas蛋白特异结合靶标DNA、调控靶标基因转录的方法及试剂盒
US11268082B2 (en) * 2017-03-23 2022-03-08 President And Fellows Of Harvard College Nucleobase editors comprising nucleic acid programmable DNA binding proteins
WO2018175712A1 (en) * 2017-03-23 2018-09-27 The Government Of The United States Of America As Represented By The Secretary Of The Army Recombinant adeno-associated virus-paraoxonase 1-ifii particles and the methods of making and using thereof
US12161694B2 (en) 2017-03-24 2024-12-10 The Broad Institute, Inc. Methods and compositions for regulating innate lymphoid cell inflammatory responses
WO2018183908A1 (en) 2017-03-31 2018-10-04 Dana-Farber Cancer Institute, Inc. Compositions and methods for treating ovarian tumors
CN108690845B (zh) * 2017-04-10 2021-04-27 中国科学院动物研究所 基因组编辑系统和方法
WO2018191663A1 (en) * 2017-04-14 2018-10-18 Monsanto Technology Llc Methods and compositions for herbicide tolerance in plants
WO2018191715A2 (en) * 2017-04-14 2018-10-18 Synthetic Genomics, Inc. Polypeptides with type v crispr activity and uses thereof
KR102746223B1 (ko) 2017-04-20 2024-12-27 이제네시스, 인크. 유전자 변형 동물의 생성 방법
WO2018195540A1 (en) * 2017-04-21 2018-10-25 The General Hospital Corporation Inducible, tunable, and multiplex human gene regulation using crispr-cpf1
CN110799525A (zh) 2017-04-21 2020-02-14 通用医疗公司 具有改变的PAM特异性的CPF1(CAS12a)的变体
EP3615672A1 (en) 2017-04-28 2020-03-04 Editas Medicine, Inc. Methods and systems for analyzing guide rna molecules
JP7292213B2 (ja) * 2017-05-04 2023-06-16 ザ トラスティーズ オブ ザ ユニバーシティ オブ ペンシルバニア Ｃｒｉｓｐｒ／ｃｐｆ１を用いる、ｔ細胞における遺伝子編集のための組成物および方法
AU2018266698A1 (en) 2017-05-08 2019-11-28 Precision Biosciences, Inc. Nucleic acid molecules encoding an engineered antigen receptor and an inhibitory nucleic acid molecule and methods of use thereof
WO2018209324A2 (en) 2017-05-11 2018-11-15 The Broad Institute, Inc. Methods and compositions of use of cd8+ tumor infiltrating lymphocyte subtypes and gene signatures thereof
WO2018209320A1 (en) 2017-05-12 2018-11-15 President And Fellows Of Harvard College Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation
US12297436B2 (en) 2017-05-18 2025-05-13 The Broad Institute, Inc. Systems, methods, and compositions for targeted nucleic acid editing
WO2018213708A1 (en) * 2017-05-18 2018-11-22 The Broad Institute, Inc. Systems, methods, and compositions for targeted nucleic acid editing
US11046969B2 (en) 2017-05-24 2021-06-29 Epiplanta Biotech Ltd. Transgenic plant and the method for producing the same
AU2018273979B2 (en) * 2017-05-25 2024-10-17 Regeneron Pharmaceuticals, Inc. CBLB endonuclease variants, compositions, and methods of use
CA3102054A1 (en) 2017-06-05 2018-12-13 Fred Hutchinson Cancer Research Center Genomic safe harbors for genetic therapies in human stem cells and engineered nanoparticles to provide targeted genetic therapies
EP3635112A2 (en) 2017-06-06 2020-04-15 Zymergen, Inc. A htp genomic engineering platform for improving fungal strains
EP3485013B1 (en) 2017-06-06 2021-03-24 Zymergen, Inc. A htp genomic engineering platform for improving escherichia coli
CN110997908A (zh) 2017-06-09 2020-04-10 爱迪塔斯医药公司 工程化的cas9核酸酶
US20200123203A1 (en) 2017-06-13 2020-04-23 Flagship Pioneering Innovations V, Inc. Compositions comprising curons and uses thereof
WO2018232195A1 (en) 2017-06-14 2018-12-20 The Broad Institute, Inc. Compositions and methods targeting complement component 3 for inhibiting tumor growth
HUE066467T2 (hu) * 2017-06-23 2024-08-28 Inscripta Inc Nukleinsav-irányított nukleázok
US9982279B1 (en) 2017-06-23 2018-05-29 Inscripta, Inc. Nucleic acid-guided nucleases
US10011849B1 (en) 2017-06-23 2018-07-03 Inscripta, Inc. Nucleic acid-guided nucleases
CN107312790A (zh) * 2017-06-26 2017-11-03 中国科学技术大学 一种可编程的多位点特异性的转录抑制系统及其应用
EP3645054A4 (en) 2017-06-26 2021-03-31 The Broad Institute, Inc. CRISPR / CAS-ADENINE DESAMINASE COMPOSITIONS, TARGETED NUCLEIC ACID EDITING SYSTEMS AND METHODS
WO2019005957A1 (en) 2017-06-30 2019-01-03 Precision Biosciences, Inc. GENETICALLY MODIFIED T CELLS COMPRISING A MODIFIED INTRON IN THE ALPHA T CELL RECEPTOR GENE
WO2019006436A1 (en) 2017-06-30 2019-01-03 Inscripta, Inc. METHODS, MODULES, INSTRUMENTS AND SYSTEMS FOR AUTOMATED CELL PROCESSING
US11168322B2 (en) 2017-06-30 2021-11-09 Arbor Biotechnologies, Inc. CRISPR RNA targeting enzymes and systems and uses thereof
JP2020530307A (ja) 2017-06-30 2020-10-22 インティマ・バイオサイエンス，インコーポレーテッド 遺伝子治療のためのアデノ随伴ウイルスベクター
KR102007457B1 (ko) * 2017-06-30 2019-08-05 동국대학교 산학협력단 Cas9 단백질, 가이드 RNA 및 양친매성 펩타이드를 포함하는 나노복합체 및 이의 용도
EP3648781A4 (en) * 2017-07-07 2021-05-19 The Broad Institute, Inc. Crispr system based antiviral therapy
WO2019014564A1 (en) 2017-07-14 2019-01-17 Editas Medicine, Inc. SYSTEMS AND METHODS OF TARGETED INTEGRATION AND GENOME EDITING AND DETECTION THEREOF WITH INTEGRATED PRIMING SITES
CN112501254B (zh) * 2017-07-14 2024-07-19 上海吐露港生物科技有限公司 一种Cas蛋白的用途及靶标核酸分子的检测方法和试剂盒
US12049643B2 (en) 2017-07-14 2024-07-30 The Broad Institute, Inc. Methods and compositions for modulating cytotoxic lymphocyte activity
US20200231975A1 (en) * 2017-07-17 2020-07-23 The Broad Institute, Inc. Novel type vi crispr orthologs and systems
WO2019018440A1 (en) 2017-07-17 2019-01-24 The Broad Institute, Inc. HUMAN COLON CELL ATLAS IN GOOD HEALTH AND WITH HEMORRHAGIC RECTO-COLITIS
WO2019017988A1 (en) * 2017-07-21 2019-01-24 Arizona Board Of Regents On Behalf Of Arizona State University CRISPR FLUORESCENT GUIDED RNA (ARNFG) FOR UNDERSTANDING RNAs EXPRESSED FROM POL II PROMOTERS
EP3657980A4 (en) 2017-07-28 2021-04-28 Locus IP Company, LLC YEAST MASKS TO IMPROVE SKIN, HAIR AND SCALP HEALTH
JP2020534795A (ja) 2017-07-28 2020-12-03 プレジデント アンド フェローズ オブ ハーバード カレッジ ファージによって支援される連続的進化（ｐａｃｅ）を用いて塩基編集因子を進化させるための方法および組成物
RU2019143568A (ru) 2017-07-31 2021-09-02 Регенерон Фармасьютикалс, Инк. Способы и композиции для оценки crispr/cas-индуцированной рекомбинации с экзогенной донорной нуклеиновой кислотой in vivo
KR20200033259A (ko) 2017-07-31 2020-03-27 리제너론 파마슈티칼스 인코포레이티드 생체내에서 CRISPR/Cas-매개된 파괴 또는 삭제 및 외인성 도너 핵산과의 CRISPR/Cas-유도된 재조합을 평가하기 위한 방법 및 조성물
AU2018309716B2 (en) 2017-07-31 2024-11-21 Regeneron Pharmaceuticals, Inc. Cas-transgenic mouse embryonic stem cells and mice and uses thereof
IL271283B2 (en) 2017-08-04 2024-04-01 Syngenta Participations Ag Methods and compositions for targeted genomic insertion
BR112020002647A2 (pt) 2017-08-09 2020-08-18 Benson Hill, Inc. composições e métodos para modificação de genomas
US10476825B2 (en) 2017-08-22 2019-11-12 Salk Institue for Biological Studies RNA targeting methods and compositions
US11970720B2 (en) 2017-08-22 2024-04-30 Salk Institute For Biological Studies RNA targeting methods and compositions
JP7581046B2 (ja) 2017-08-23 2024-11-12 ザ ジェネラル ホスピタル コーポレイション 変更されたＰＡＭ特異性を有する遺伝子操作されたＣＲＩＳＰＲ－Ｃａｓ９ヌクレアーゼ
US10738327B2 (en) 2017-08-28 2020-08-11 Inscripta, Inc. Electroporation cuvettes for automation
WO2019139645A2 (en) 2017-08-30 2019-07-18 President And Fellows Of Harvard College High efficiency base editors comprising gam
WO2019046540A1 (en) * 2017-08-31 2019-03-07 New York Genome Center, Inc. METHODS AND COMPOSITIONS COMPRISING CRISPR-CPF1 AND APPROVED GUIDING CRISPR RNAES FOR PROGRAMMABLE GENOMIC DELETIONS
KR102338449B1 (ko) * 2017-09-21 2021-12-10 더 브로드 인스티튜트, 인코퍼레이티드 표적화된 핵산 편집을 위한 시스템, 방법, 및 조성물
EP4276185A3 (en) 2017-09-29 2024-02-21 Regeneron Pharmaceuticals, Inc. Rodents comprising a humanized ttr locus and methods of use
KR102662879B1 (ko) * 2017-09-29 2024-05-02 주식회사 툴젠 망막 기능장애 질환 치료를 위한 유전자 조작
WO2019068022A1 (en) 2017-09-30 2019-04-04 Inscripta, Inc. CONTINUOUS FLOW ELECTROPORATION INSTRUMENTATION
US20200239544A1 (en) 2017-10-03 2020-07-30 Precision Biosciences, Inc. Modified epidermal growth factor receptor peptides for use in genetically-modified cells
EP3692063A1 (en) 2017-10-03 2020-08-12 Juno Therapeutics, Inc. Hpv-specific binding molecules
RU2020115264A (ru) * 2017-10-04 2021-11-08 Зе Броад Институт, Инк. Диагностика на основе эффекторной системы crispr
CA3082251A1 (en) 2017-10-16 2019-04-25 The Broad Institute, Inc. Uses of adenosine base editors
CN111479916B (zh) 2017-10-20 2024-10-22 弗莱德哈钦森癌症中心 用以产生被遗传修饰来表达所选抗体的b细胞的系统和方法
WO2019089913A1 (en) 2017-11-01 2019-05-09 Precision Biosciences, Inc. Engineered nucleases that target human and canine factor viii genes as a treatment for hemophilia a
WO2019089804A1 (en) 2017-11-01 2019-05-09 The Regents Of The University Of California Casy compositions and methods of use
AU2018358051B2 (en) * 2017-11-01 2025-01-09 The Regents Of The University Of California CasZ compositions and methods of use
US11970719B2 (en) 2017-11-01 2024-04-30 The Regents Of The University Of California Class 2 CRISPR/Cas compositions and methods of use
EP3704237A1 (en) 2017-11-02 2020-09-09 Arbor Biotechnologies, Inc. Novel crispr-associated transposon systems and components
GB2568255A (en) * 2017-11-08 2019-05-15 Evox Therapeutics Ltd Exosomes comprising RNA therapeutics
US12171783B2 (en) 2017-11-13 2024-12-24 The Broad Institute, Inc. Methods and compositions for targeting developmental and oncogenic programs in H3K27M gliomas
US12221720B2 (en) 2017-11-13 2025-02-11 The Broad Institute, Inc. Methods for determining spatial and temporal gene expression dynamics during adult neurogenesis in single cells
CN111448313A (zh) * 2017-11-16 2020-07-24 阿斯利康(瑞典)有限公司 用于改善基于Cas9的敲入策略的有效性的组合物和方法
BR112020009663A2 (pt) 2017-11-17 2020-11-10 Iovance Biotherapeutics, Inc. método para a expansão de linfócitos infiltrantes de tumor (tils) em uma população terapêutica de tils, método para o tratamento de um indivíduo com câncer, composição
EP3714041A1 (en) 2017-11-22 2020-09-30 Iovance Biotherapeutics, Inc. Expansion of peripheral blood lymphocytes (pbls) from peripheral blood
US10253365B1 (en) * 2017-11-22 2019-04-09 The Regents Of The University Of California Type V CRISPR/Cas effector proteins for cleaving ssDNAs and detecting target DNAs
WO2019108670A1 (en) * 2017-11-28 2019-06-06 Emendobio Inc. Differential knockout of an allele of a heterozygous fibrinogen alpha chain (fga) gene
US11332736B2 (en) 2017-12-07 2022-05-17 The Broad Institute, Inc. Methods and compositions for multiplexing single cell and single nuclei sequencing
WO2019113463A1 (en) 2017-12-08 2019-06-13 Synthetic Genomics, Inc. Improving algal lipid productivity via genetic modification of a tpr domain containing protein
US11203752B2 (en) * 2017-12-11 2021-12-21 Pioneer Hi-Bred International, Inc. Compositions and methods of modifying a plant genome to produce a MS9, MS22, MS26, or MS45 male-sterile plant
EP3725092A4 (en) 2017-12-14 2021-09-22 FloDesign Sonics, Inc. DRIVE AND CONTROL UNIT FOR ACOUSTIC CONVERTER
US12406749B2 (en) 2017-12-15 2025-09-02 The Broad Institute, Inc. Systems and methods for predicting repair outcomes in genetic engineering
CN112119085B (zh) 2017-12-15 2025-04-01 丹娜-法伯癌症研究院有限公司 稳定肽-介导的靶向蛋白降解
US11293019B2 (en) 2017-12-22 2022-04-05 Gflas Life Sciences, Inc. Chimeric genome engineering molecules and methods
SG11202005173SA (en) 2017-12-22 2020-06-29 Fate Therapeutics Inc Enhanced immune effector cells and use thereof
US20230193242A1 (en) * 2017-12-22 2023-06-22 The Broad Institute, Inc. Cas12b systems, methods, and compositions for targeted dna base editing
US11994512B2 (en) 2018-01-04 2024-05-28 Massachusetts Institute Of Technology Single-cell genomic methods to generate ex vivo cell systems that recapitulate in vivo biology with improved fidelity
BR112020014168A2 (pt) 2018-01-12 2020-12-08 Basf Se Proteína, ácido nucleico isolado, gene recombinante, vetor, célula hospedeira, planta, parte de planta ou semente de trigo, métodos para produzir, produto de trigo, farinha, farelo integral, amido, grânulos de amido ou farelo de trigo e métodos para identificar e/ou selecionar uma planta de trigo
CN111742051A (zh) * 2018-01-23 2020-10-02 基础科学研究院 延伸的单向导rna及其用途
US11926835B1 (en) 2018-01-29 2024-03-12 Inari Agriculture Technology, Inc. Methods for efficient tomato genome editing
US11220694B1 (en) * 2018-01-29 2022-01-11 Inari Agriculture, Inc. Rice cells and rice plants
WO2019152406A1 (en) * 2018-01-30 2019-08-08 The University Of Memphis Research Foundation Compositions and methods for regulating a biological process
US12275951B2 (en) * 2018-02-06 2025-04-15 Ohio State Innovation Foundation Engineered guide RNA and uses thereof
CN111788307A (zh) * 2018-03-12 2020-10-16 南京北恒生物科技有限公司 工程化的嵌合向导rna及其用途
CN112041444A (zh) 2018-03-14 2020-12-04 阿伯生物技术公司 新型crispr dna靶向酶及系统
DK3765616T3 (da) 2018-03-14 2023-08-21 Arbor Biotechnologies Inc Nye enzymer og systemer målrettet crispr dna og rna
JP2021518162A (ja) * 2018-03-15 2021-08-02 ケーエスキュー セラピューティクス， インコーポレイテッド 免疫療法の改善のための遺伝子調節組成物及び遺伝子調節方法
CN108486146B (zh) * 2018-03-16 2021-02-19 中国农业科学院作物科学研究所 LbCpf1-RR突变体用于CRISPR/Cpf1系统在植物基因编辑中的应用
WO2019183123A1 (en) 2018-03-19 2019-09-26 Regeneron Pharmaceuticals, Inc. Transcription modulation in animals using crispr/cas systems
EP3546582A1 (en) 2018-03-26 2019-10-02 KWS SAAT SE & Co. KGaA Promoter activating elements
WO2019190874A1 (en) 2018-03-29 2019-10-03 Inscripta, Inc. Automated control of cell growth rates for induction and transformation
EP3775228A4 (en) * 2018-03-29 2022-02-23 Fate Therapeutics, Inc. ENHANCED IMMUNE EFFECTIVE CELLS AND THEIR USE
JP7589047B2 (ja) 2018-04-05 2024-11-25 ジュノー セラピューティクス インコーポレイテッド 組換え受容体を発現する細胞の作製方法および関連組成物
WO2019195491A1 (en) 2018-04-05 2019-10-10 Juno Therapeutics, Inc. T cells expressing a recombinant receptor, related polynucleotides and methods
WO2019200122A1 (en) 2018-04-12 2019-10-17 Precision Biosciences, Inc. Optimized engineered nucleases having specificity for the human t cell receptor alpha constant region gene
WO2019200004A1 (en) 2018-04-13 2019-10-17 Inscripta, Inc. Automated cell processing instruments comprising reagent cartridges
US20210189361A1 (en) * 2018-04-23 2021-06-24 Duke University Downregulation of snca expression by targeted editing of dna-methylation
US10858761B2 (en) 2018-04-24 2020-12-08 Inscripta, Inc. Nucleic acid-guided editing of exogenous polynucleotides in heterologous cells
US10557216B2 (en) 2018-04-24 2020-02-11 Inscripta, Inc. Automated instrumentation for production of T-cell receptor peptide libraries
PL3560330T3 (pl) 2018-04-24 2022-09-19 KWS SAAT SE & Co. KGaA Rośliny o ulepszonej strawności i haplotypach markerowych
WO2019209926A1 (en) 2018-04-24 2019-10-31 Inscripta, Inc. Automated instrumentation for production of peptide libraries
US11957695B2 (en) 2018-04-26 2024-04-16 The Broad Institute, Inc. Methods and compositions targeting glucocorticoid signaling for modulating immune responses
US20210130779A1 (en) 2018-04-27 2021-05-06 Iovance Biotherapeutics, Inc. Closed process for expansion and gene editing of tumor infiltrating lymphocytes and uses of same in immunotherapy
CN109593763B (zh) * 2018-04-27 2021-10-29 四川大学华西医院 一种FnCpf1介导的体外DNA编辑试剂盒
EP3799603A4 (en) 2018-05-11 2022-03-02 Beam Therapeutics, Inc. METHODS OF PATHOGENIC AMINO ACID SUBSTITUTION USING PROGRAMMABLE BASE EDITOR SYSTEMS
CN112601816B (zh) * 2018-05-11 2024-12-17 比姆医疗股份有限公司 使用可编程碱基编辑器系统遏止病原性突变的方法
WO2019222555A1 (en) 2018-05-16 2019-11-21 Arbor Biotechnologies, Inc. Novel crispr-associated systems and components
US12157760B2 (en) 2018-05-23 2024-12-03 The Broad Institute, Inc. Base editors and uses thereof
US11833225B2 (en) * 2018-05-24 2023-12-05 Crispr Therapeutics Ag Methods and compositions for efficient gene deletion
WO2019232251A1 (en) * 2018-05-30 2019-12-05 The Regents Of The University Of California Gene editing of monogenic disorders in human hematopoietic stem cells -- correction of x-linked hyper-igm syndrome (xhim)
EP3575402A1 (en) * 2018-06-01 2019-12-04 Algentech SAS Gene targeting
CA3102676A1 (en) 2018-06-04 2019-12-12 University Of Copenhagen Mutant cpf1 endonucleases
US11866719B1 (en) 2018-06-04 2024-01-09 Inari Agriculture Technology, Inc. Heterologous integration of regulatory elements to alter gene expression in wheat cells and wheat plants
IL279222B2 (en) 2018-06-05 2025-10-01 Lifeedit Inc RNA-guided nucleases and active variant fragments thereof and methods of use
US12048296B2 (en) 2018-06-07 2024-07-30 Korea Research Institute Of Bioscience And Biotechnology Transgenic mouse for aglycosylated antibody production and use of aglycosylated antibody produced therefrom
WO2019237035A1 (en) 2018-06-08 2019-12-12 Intellia Therapeutics, Inc. Compositions and methods for immunooncology
US12227776B2 (en) 2018-06-13 2025-02-18 Caribou Biosciences, Inc. Engineered cascade components and cascade complexes
US10227576B1 (en) 2018-06-13 2019-03-12 Caribou Biosciences, Inc. Engineered cascade components and cascade complexes
US12036240B2 (en) 2018-06-14 2024-07-16 The Broad Institute, Inc. Compositions and methods targeting complement component 3 for inhibiting tumor growth
WO2019238911A1 (en) 2018-06-15 2019-12-19 KWS SAAT SE & Co. KGaA Methods for improving genome engineering and regeneration in plant ii
AU2019285083B2 (en) 2018-06-15 2024-01-25 KWS SAAT SE & Co. KGaA Methods for improving genome engineering and regeneration in plant
WO2019237395A1 (zh) * 2018-06-16 2019-12-19 深圳市博奥康生物科技有限公司 一种应用CRISPR/Cas9系统靶向敲除人CD357基因的方法
WO2019237380A1 (zh) * 2018-06-16 2019-12-19 深圳市博奥康生物科技有限公司 一种基于慢病毒的 CRISPR/Cpf1 基因编辑载体及其应用
BR112020026306A2 (pt) 2018-06-26 2021-03-30 The Broad Institute Inc. Métodos de amplificação, sistemas e diagnósticos baseados em sistema efeitor crispr
AU2019291827A1 (en) 2018-06-26 2020-12-24 Massachusetts Institute Of Technology Crispr double nickase based amplification compositions, systems, and methods
EP3521436A1 (en) 2018-06-27 2019-08-07 VIB vzw Complex breeding in plants
CA3104856A1 (en) 2018-06-29 2020-01-02 Editas Medicine, Inc. Synthetic guide molecules, compositions and methods relating thereto
WO2020018142A1 (en) 2018-07-16 2020-01-23 Arbor Biotechnologies, Inc. Novel crispr dna targeting enzymes and systems
WO2020018166A1 (en) 2018-07-16 2020-01-23 The Regents Of The University Of Michigan Nuclease-mediated nucleic acid modification
EP4442836A3 (en) 2018-08-01 2024-12-18 Mammoth Biosciences, Inc. Programmable nuclease compositions and methods of use thereof
AU2019319377A1 (en) * 2018-08-09 2021-03-11 G+Flas Life Sciences Novel CRISPR-associated protein and use thereof
CA3109083A1 (en) 2018-08-09 2020-02-13 G+Flas Life Sciences Compositions and methods for genome engineering with cas12a proteins
US10533152B1 (en) 2018-08-14 2020-01-14 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
US10532324B1 (en) 2018-08-14 2020-01-14 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
US10752874B2 (en) 2018-08-14 2020-08-25 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
US11142740B2 (en) 2018-08-14 2021-10-12 Inscripta, Inc. Detection of nuclease edited sequences in automated modules and instruments
US12497611B2 (en) 2018-08-17 2025-12-16 Yale University Compositions and methods for high-throughput activation screening to boost T cell effector function
WO2020041380A1 (en) 2018-08-20 2020-02-27 The Broad Institute, Inc. Methods and compositions for optochemical control of crispr-cas9
WO2020041782A1 (en) * 2018-08-24 2020-02-27 Flagship Pioneering Innovations Vi, Llc Methods and compositions for the modification of plants
AR116016A1 (es) * 2018-08-24 2021-03-25 Flagship Pioneering Innovations Vi Llc Métodos para fabricar paquetes mensajeros vegetales
AU2019363487A1 (en) 2018-08-30 2021-04-15 Inscripta, Inc. Improved detection of nuclease edited sequences in automated modules and instruments
US11479762B1 (en) 2018-08-31 2022-10-25 Inari Agriculture Technology, Inc. Compositions, systems, and methods for genome editing
CN109402115B (zh) * 2018-09-06 2024-02-02 广州普世利华科技有限公司 靶向Rett突变基因RNA的gRNA及Rett突变基因的检测方法、检测试剂盒
WO2020051507A1 (en) 2018-09-06 2020-03-12 The Broad Institute, Inc. Nucleic acid assemblies for use in targeted delivery
US20210198642A1 (en) 2018-09-07 2021-07-01 Astrazeneca Ab Compositions and methods for improved nucleases
US12454694B2 (en) 2018-09-07 2025-10-28 Beam Therapeutics Inc. Compositions and methods for improving base editing
EP3849565A4 (en) 2018-09-12 2022-12-28 Fred Hutchinson Cancer Research Center REDUCTION OF CD33 EXPRESSION TO SELECTIVELY PROTECT THERAPEUTIC CELLS
WO2020055941A1 (en) * 2018-09-13 2020-03-19 Excision Biotherapeutics, Inc. Compositions and methods for excision with single grna
US11459552B2 (en) * 2018-09-13 2022-10-04 The Board Of Regents Of The University Of Oklahoma Variant CAS12 proteins with improved dna cleavage selectivity and methods of use
US12331320B2 (en) 2018-10-10 2025-06-17 The Research Foundation For The State University Of New York Genome edited cancer cell vaccines
CN113260701A (zh) 2018-10-18 2021-08-13 英特利亚治疗股份有限公司 用于表达因子ix的组合物和方法
US11214781B2 (en) 2018-10-22 2022-01-04 Inscripta, Inc. Engineered enzyme
WO2020086475A1 (en) 2018-10-22 2020-04-30 Inscripta, Inc. Engineered enzymes
CN111100876A (zh) * 2018-10-25 2020-05-05 立沃生物科技（深圳）有限公司 CRISPR-Cas9特异性敲除FAH基因的方法及特异性sgRNA
US11407995B1 (en) 2018-10-26 2022-08-09 Inari Agriculture Technology, Inc. RNA-guided nucleases and DNA binding proteins
WO2020092453A1 (en) 2018-10-29 2020-05-07 The Broad Institute, Inc. Nucleobase editors comprising geocas9 and uses thereof
US20210388389A1 (en) 2018-10-30 2021-12-16 Yale University Compositions and methods for rapid and modular generation of chimeric antigen receptor t cells
KR20210088615A (ko) 2018-10-31 2021-07-14 지머젠 인코포레이티드 Dna 라이브러리의 다중 결정적 어셈블리
US20220333089A1 (en) * 2018-11-01 2022-10-20 The University Of Tokyo Split CPF1 Protein
US11434477B1 (en) 2018-11-02 2022-09-06 Inari Agriculture Technology, Inc. RNA-guided nucleases and DNA binding proteins
SG11202104630PA (en) 2018-11-05 2021-06-29 Iovance Biotherapeutics Inc Selection of improved tumor reactive t-cells
ES3036072T3 (en) 2018-11-05 2025-09-12 Iovance Biotherapeutics Inc Processes for production of tumor infiltrating lymphocytes and uses of the same in immunotherapy
MX2021004775A (es) 2018-11-05 2021-06-08 Iovance Biotherapeutics Inc Expansion de linfocitos infiltrantes de tumores (tils) usando inhibidores de la via de proteina cinasa b (akt).
CA3118634A1 (en) 2018-11-05 2020-05-14 Iovance Biotherapeutics, Inc. Treatment of nsclc patients refractory for anti-pd-1 antibody
CN109385451B (zh) * 2018-11-07 2020-07-07 中国海洋大学 一种牡蛎CRISPR/Cas9基因编辑方法
CN118497276A (zh) * 2018-11-07 2024-08-16 阿库斯股份有限公司 腺相关病毒载体用于在内耳中的毛细胞和支持细胞中校正基因缺陷/表达蛋白质的用途
AU2019375914A1 (en) * 2018-11-08 2021-06-03 Arizona Board of Regents on Behalf Arizona State University Synthetic immunomodulation with a CRISPR super-repressor in vivo
US12165743B2 (en) 2018-11-09 2024-12-10 The Broad Institute, Inc. Compressed sensing for screening and tissue imaging
US12402610B2 (en) 2018-11-09 2025-09-02 The Broad Institute, Inc. Methods and compositions for modulating innate lymphoid cell pathogenic effectors
US12460189B2 (en) 2018-11-09 2025-11-04 Inari Agriculture Technology, Inc. RNA-guided nucleases and DNA binding proteins
BR112021009425A2 (pt) * 2018-11-14 2021-11-23 Massachusetts Inst Technology Sistemas e métodos de diagnóstico por gotículas com base no sistema crispr
JP7460178B2 (ja) * 2018-11-15 2024-04-02 中国▲農▼▲業▼大学 ＣＲＩＳＰＲ－Ｃａｓ１２ｊ酵素およびシステム
CN113015798B (zh) * 2018-11-15 2023-01-10 中国农业大学 CRISPR-Cas12a酶和系统
CN109536506B (zh) * 2018-12-10 2022-03-22 潍坊科技学院 一种调控日本沼虾生长发育的类胰岛素受体基因及其应用
CN109593743A (zh) * 2018-12-12 2019-04-09 广州普世利华科技有限公司 新型CRISPR/ScCas12a蛋白及其制备方法
CN109680053A (zh) * 2018-12-12 2019-04-26 广州普世利华科技有限公司 新型SsCas12a蛋白在核酸检测方面的应用
US11166996B2 (en) 2018-12-12 2021-11-09 Flagship Pioneering Innovations V, Inc. Anellovirus compositions and methods of use
CN109666662A (zh) * 2018-12-12 2019-04-23 广州普世利华科技有限公司 新型ScCas12a在核酸检测方面的应用
WO2020124050A1 (en) 2018-12-13 2020-06-18 The Broad Institute, Inc. Tiled assays using crispr-cas based detection
US12264323B2 (en) 2018-12-17 2025-04-01 The Broad Institute, Inc. CRISPR CPF1 direct repeat variants
CN113544266A (zh) 2018-12-17 2021-10-22 博德研究所 Crispr相关转座酶系统和其使用方法
JP7710372B2 (ja) 2018-12-19 2025-07-18 アイオバンス バイオセラピューティクス，インコーポレイテッド 操作されたサイトカイン受容体対を使用して腫瘍浸潤リンパ球を拡大培養する方法及びその使用
JP7449291B2 (ja) 2018-12-20 2024-03-13 リジェネロン・ファーマシューティカルズ・インコーポレイテッド ヌクレアーゼ媒介リピート伸長
US20220090047A1 (en) 2018-12-21 2022-03-24 Precision Biosciences, Inc. Genetic modification of the hydroxyacid oxidase 1 gene for treatment of primary hyperoxaluria
MX2021007835A (es) 2018-12-27 2021-08-11 Lifeedit Therapeutics Inc Polipeptidos utiles para edicion de genes y metodos de uso.
EP3931313A2 (en) 2019-01-04 2022-01-05 Mammoth Biosciences, Inc. Programmable nuclease improvements and compositions and methods for nucleic acid amplification and detection
US11739156B2 (en) 2019-01-06 2023-08-29 The Broad Institute, Inc. Massachusetts Institute of Technology Methods and compositions for overcoming immunosuppression
US12351837B2 (en) 2019-01-23 2025-07-08 The Broad Institute, Inc. Supernegatively charged proteins and uses thereof
CN109799295A (zh) * 2019-01-28 2019-05-24 江南大学 一种用于秀丽隐杆线虫代谢组学分析的样品前处理方法
WO2020163856A1 (en) 2019-02-10 2020-08-13 The J. David Gladstone Institutes, A Testamentary Trust Established Under The Will Of J. David Gladstone Modified mitochondrion and methods of use thereof
EA202192233A1 (ru) 2019-02-12 2021-11-11 Университа' Дельи Студи Ди Тренто МОЛЕКУЛЫ ГИДОВОЙ РНК Cas12a И ИХ ПРИМЕНЕНИЕ
CN113439121B (zh) * 2019-02-13 2024-12-06 普罗拜奥根股份公司 具有增强的插入位点选择特性的转座酶
CN116497067B (zh) 2019-02-13 2025-01-24 比姆医疗股份有限公司 治疗血红素病变的组合物和方法
CN114190093B (zh) * 2019-02-13 2025-01-17 比姆医疗股份有限公司 使用腺苷酸脱氨酶碱基编辑器破坏疾病相关基因的剪接受体位点，包括用于治疗遗传性疾病
WO2020168279A2 (en) * 2019-02-15 2020-08-20 Allen Institute Artificial expression constructs for selectively modulating gene expression in selected neuronal cell populations
JP7407830B2 (ja) * 2019-02-22 2024-01-04 インテグレーティッド ディーエヌエイ テクノロジーズ インコーポレーティッド Ｌａｃｈｎｏｓｐｉｒａｃｅａｅ ｂａｃｔｅｒｉｕｍ ＮＤ２００６のＣＡＳ１２Ａ変異型遺伝子およびそれらによってコードされるポリペプチド
CN109975543B (zh) * 2019-03-01 2022-02-11 中国疾病预防控制中心传染病预防控制所 分枝杆菌Ku蛋白的应用
BR112021016875A2 (pt) 2019-03-01 2022-01-04 Iovance Biotherapeutics Inc Processo para expansão de linfócitos de sangue periférico
JP7649748B2 (ja) * 2019-03-07 2025-03-21 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア Ｃｒｉｓｐｒ－ｃａｓエフェクターポリペプチド及びその使用方法
KR20210136997A (ko) 2019-03-08 2021-11-17 지머젠 인코포레이티드 미생물에서 반복적 게놈 편집
US11053515B2 (en) 2019-03-08 2021-07-06 Zymergen Inc. Pooled genome editing in microbes
CA3133226A1 (en) 2019-03-11 2020-09-17 Sorrento Therapeutics, Inc. Improved process for integration of dna constructs using rna-guided endonucleases
US20220154258A1 (en) 2019-03-14 2022-05-19 The Broad Institute, Inc. Crispr effector system based multiplex diagnostics
AU2020241856B2 (en) 2019-03-18 2024-06-27 Regeneron Pharmaceuticals, Inc. CRISPR/Cas dropout screening platform to reveal genetic vulnerabilities associated with tau aggregation
AU2020241858B2 (en) 2019-03-18 2024-06-13 Regeneron Pharmaceuticals, Inc. CRISPR/Cas screening platform to identify genetic modifiers of tau seeding or aggregation
AU2020240109A1 (en) 2019-03-19 2021-09-30 President And Fellows Of Harvard College Methods and compositions for editing nucleotide sequences
US11001831B2 (en) 2019-03-25 2021-05-11 Inscripta, Inc. Simultaneous multiplex genome editing in yeast
EP3947691A4 (en) 2019-03-25 2022-12-14 Inscripta, Inc. SIMULTANEOUS MULTIPLEX GENOME EDITING IN A YEAST
KR20250148676A (ko) 2019-04-03 2025-10-14 리제너론 파마슈티칼스 인코포레이티드 세이프 하버 좌위 내로의 항체 코딩 서열의 삽입을 위한 방법 및 조성물
IL316764A (en) 2019-04-03 2025-01-01 Prec Biosciences Inc Genetically modified immune cells containing shRNA-targeted microRNA (shRNAmir)
IL286905B2 (en) 2019-04-04 2024-06-01 Regeneron Pharma Non-human animals containing the human coagulation factor 12 locus
IL286917B (en) 2019-04-04 2022-09-01 Regeneron Pharma Methods for scarless introduction of targeted modifications into targeting vectors
JP7426120B2 (ja) 2019-04-05 2024-02-01 国立大学法人大阪大学 ノックイン細胞の作製方法
CA3136265A1 (en) 2019-04-05 2020-10-08 Precision Biosciences, Inc. Methods of preparing populations of genetically-modified immune cells
WO2020214842A1 (en) 2019-04-17 2020-10-22 The Broad Institute, Inc. Adenine base editors with reduced off-target effects
MX2021012886A (es) * 2019-04-25 2022-01-04 Flagship Pioneering Innovations Vi Llc Composiciones y metodos relacionados con paquetes mensajeros de plantas.
CN110396543A (zh) * 2019-04-30 2019-11-01 广州普世利华科技有限公司 一种肿瘤相关基因突变位点筛查方法
CN110195102A (zh) * 2019-04-30 2019-09-03 广州普世利华科技有限公司 一种β地中海贫血基因分型方法
CA3138329A1 (en) 2019-05-10 2020-11-19 Basf Se Regulatory nucleic acid molecules for enhancing gene expression in plants
EP3969607A1 (en) 2019-05-13 2022-03-23 KWS SAAT SE & Co. KGaA Drought tolerance in corn
WO2020232029A1 (en) 2019-05-13 2020-11-19 Iovance Biotherapeutics, Inc. Methods and compositions for selecting tumor infiltrating lymphocytes and uses of the same in immunotherapy
CN110172442B (zh) * 2019-05-16 2022-05-13 上海苹谱医疗科技有限公司 一种人诱导多潜能干细胞、构建方法及其用途
US20220220469A1 (en) * 2019-05-20 2022-07-14 The Broad Institute, Inc. Non-class i multi-component nucleic acid targeting systems
WO2020234468A1 (en) 2019-05-23 2020-11-26 Nomad Bioscience Gmbh Rna viral rna molecule for gene editing
AR118995A1 (es) 2019-05-25 2021-11-17 Kws Saat Se & Co Kgaa Mejorador de la inducción de haploides
MA56037A (fr) * 2019-05-28 2022-04-06 Octant Inc Système de relais de transcription
GB201907733D0 (en) * 2019-05-31 2019-07-17 Governing Council Of The Univ Of Toronto Methods and compositions for multiplex gene editing
KR20220016869A (ko) 2019-06-04 2022-02-10 리제너론 파마슈티칼스 인코포레이티드 베타-슬립 돌연변이를 갖는 인간화 ttr 좌위를 포함하는 비-인간 동물 및 사용 방법
US10837021B1 (en) 2019-06-06 2020-11-17 Inscripta, Inc. Curing for recursive nucleic acid-guided cell editing
BR112021024288A2 (pt) 2019-06-07 2022-02-15 Scribe Therapeutics Inc Sistemas casx modificados
BR112021022722A2 (pt) * 2019-06-07 2022-01-04 Regeneron Pharma Animal não humano, célula de animal não humana, genoma de animal não humano, gene de albumina animal não humana humanizada, vetor de alvejamento, método de avaliação da atividade de um reagente, e, método de otimização da atividade de um reagente
WO2020249593A1 (en) 2019-06-13 2020-12-17 Nunhems B.V. Tomato plant producing fruit having improved ripening characteristics
KR20220024053A (ko) * 2019-06-14 2022-03-03 리제너론 파마슈티칼스 인코포레이티드 타우병증의 모델
US10907125B2 (en) 2019-06-20 2021-02-02 Inscripta, Inc. Flow through electroporation modules and instrumentation
EP3986909A4 (en) 2019-06-21 2023-08-02 Inscripta, Inc. Genome-wide rationally-designed mutations leading to enhanced lysine production in e. coli
AU2020301236A1 (en) 2019-06-25 2021-11-25 Inari Agriculture Technology, Inc. Improved homology dependent repair genome editing
US10927385B2 (en) 2019-06-25 2021-02-23 Inscripta, Inc. Increased nucleic-acid guided cell editing in yeast
KR20220038362A (ko) 2019-07-02 2022-03-28 프레드 헛친슨 켄서 리서치 센터 재조합 ad35 벡터 및 관련 유전자 요법 개선
CN112168808B (zh) * 2019-07-02 2023-01-24 深圳市第二人民医院 一种基于crispr的细胞核靶向药物递送系统
AU2020301025A1 (en) * 2019-07-03 2022-01-20 Integrated Dna Technologies, Inc. Identification, characterization, and quantitation of CRISPR-introduced double-stranded DNA break repairs
CA3145863A1 (en) 2019-07-05 2021-01-14 Limagrain Europe Method for increasing yield in plants
CA3146967A1 (en) * 2019-07-17 2021-01-21 Fate Therapeutics, Inc. Immune effector cell engineering and use thereof
EP4004216A1 (en) 2019-07-25 2022-06-01 Precision BioSciences, Inc. Compositions and methods for sequential stacking of nucleic acid sequences into a genomic locus
WO2021021532A1 (en) * 2019-07-26 2021-02-04 Mammoth Biosciences, Inc. Compositions for detection of dna and methods of use thereof
EP4002993A4 (en) 2019-07-30 2024-01-10 Pairwise Plants Services, Inc. MORPHOGENIC REGULATORS AND METHODS OF USE
CN110358767B (zh) * 2019-07-30 2021-06-08 湖北大学 一种基于CRISPR-Cas12a系统的运动发酵单胞菌基因组编辑方法及其应用
JP7765377B2 (ja) * 2019-08-02 2025-11-06 モンサント テクノロジー エルエルシー Ｈｕｈエンドヌクレアーゼを用いた標的化ゲノム修飾を促進するための方法及び組成物
EP3772542A1 (en) 2019-08-07 2021-02-10 KWS SAAT SE & Co. KGaA Modifying genetic variation in crops by modulating the pachytene checkpoint protein 2
WO2021028359A1 (en) 2019-08-09 2021-02-18 Sangamo Therapeutics France Controlled expression of chimeric antigen receptors in t cells
CN118813654A (zh) 2019-08-12 2024-10-22 生命编辑制药股份有限公司 Rna指导的核酸酶及其活性片段及变体以及使用方法
CN110592014A (zh) * 2019-08-14 2019-12-20 广东美赛尔细胞生物科技有限公司 一种在nk细胞治疗中免辐照体外体内持续去除饲养细胞的方法
US20230056843A1 (en) * 2019-08-19 2023-02-23 Southern Medical University Construction of high-fidelity crispr/ascpf1 mutant and uses thereof
WO2021035054A1 (en) 2019-08-20 2021-02-25 Precision Biosciences, Inc. Lymphodepletion dosing regimens for cellular immunotherapies
WO2021035170A1 (en) 2019-08-21 2021-02-25 Precision Biosciences, Inc. Compositions and methods for tcr reprogramming using fusion proteins
CN112442515B (zh) * 2019-09-02 2023-01-10 南京启真基因工程有限公司 gRNA靶点组合在构建血友病模型猪细胞系中的应用
WO2021046257A1 (en) 2019-09-03 2021-03-11 The Broad Institute, Inc. Crispr effector system based multiplex cancer diagnostics
AU2020341711A1 (en) * 2019-09-05 2022-03-03 Arbor Biotechnologies, Inc. Novel CRISPR DNA targeting enzymes and systems
CN114729381A (zh) * 2019-09-05 2022-07-08 本森希尔股份有限公司 修饰基因组的组合物和方法
CA3150454A1 (en) 2019-09-09 2021-03-18 Arbor Biotechnologies, Inc. Novel crispr dna targeting enzymes and systems
MX2022002919A (es) 2019-09-10 2022-09-09 Science Solutions Llc Nuevas endonucleasas guiadas por rna crispr-cas tipo ii y tipo v de clase 2.
CN110628816B (zh) * 2019-09-12 2022-10-18 中国医学科学院血液病医院(中国医学科学院血液学研究所) 一种用于血友病a的双切供体及其药物组合
US12492406B2 (en) 2019-09-12 2025-12-09 Basf Se Regulatory nucleic acid molecules for enhancing gene expression in plants
US20210079394A1 (en) 2019-09-13 2021-03-18 Regeneron Pharmaceuticals, Inc. Transcription modulation in animals using crispr/cas systems delivered by lipid nanoparticles
CN110600083B (zh) * 2019-09-20 2020-05-19 中国人民解放军军事科学院军事医学研究院 基于无拼接组装wgs数据的醋酸钙—鲍曼不动杆菌复合群鉴定方法
CN114729376A (zh) 2019-09-23 2022-07-08 欧米茄治疗公司 用于调节肝细胞核因子4α(HNF4α)基因表达的组合物和方法
CA3147641A1 (en) 2019-09-23 2021-04-01 Omega Therapeutics, Inc. Compositions and methods for modulating apolipoprotein b (apob) gene expression
CA3153197A1 (en) * 2019-10-03 2021-04-08 Ryan T. Gill Crispr systems with engineered dual guide nucleic acids
WO2021069387A1 (en) 2019-10-07 2021-04-15 Basf Se Regulatory nucleic acid molecules for enhancing gene expression in plants
US12435330B2 (en) 2019-10-10 2025-10-07 The Broad Institute, Inc. Methods and compositions for prime editing RNA
WO2021074367A1 (en) 2019-10-17 2021-04-22 KWS SAAT SE & Co. KGaA Enhanced disease resistance of crops by downregulation of repressor genes
CN114829595A (zh) * 2019-10-17 2022-07-29 成对植物服务股份有限公司 Cas12a核酸酶的变体及其制备方法和用途
WO2021081200A1 (en) 2019-10-22 2021-04-29 Inari Agriculture, Inc. Genomic alteration of plant germline
CN115279898A (zh) 2019-10-23 2022-11-01 成对植物服务股份有限公司 用于植物中rna模板化编辑的组合物和方法
EP4048295A1 (en) 2019-10-25 2022-08-31 Iovance Biotherapeutics, Inc. Gene editing of tumor infiltrating lymphocytes and uses of same in immunotherapy
WO2021081384A1 (en) * 2019-10-25 2021-04-29 Greenvenus, Llc Synthetic nucleases
CN114867852A (zh) 2019-10-30 2022-08-05 成对植物服务股份有限公司 V型crispr-cas碱基编辑器及其使用方法
WO2021087305A1 (en) 2019-10-30 2021-05-06 Precision Biosciences, Inc. Cd20 chimeric antigen receptors and methods of use for immunotherapy
US11844800B2 (en) 2019-10-30 2023-12-19 Massachusetts Institute Of Technology Methods and compositions for predicting and preventing relapse of acute lymphoblastic leukemia
US20230002746A1 (en) * 2019-10-31 2023-01-05 Inari Agriculture Technology, Inc. Base-editing systems
KR20220110748A (ko) 2019-11-05 2022-08-09 페어와이즈 플랜츠 서비시즈, 인크. 대립유전자의 rna-코딩된 dna-대체를 위한 조성물 및 방법
EP4054651A1 (en) 2019-11-08 2022-09-14 Regeneron Pharmaceuticals, Inc. Crispr and aav strategies for x-linked juvenile retinoschisis therapy
CN115052980B (zh) * 2019-11-18 2024-09-10 苏州齐禾生科生物科技有限公司 衍生自黄杆菌的基因编辑系统
US11203762B2 (en) 2019-11-19 2021-12-21 Inscripta, Inc. Methods for increasing observed editing in bacteria
CN110760597B (zh) * 2019-11-22 2022-08-23 西北农林科技大学 一种检测黄牛ncstn基因拷贝数变异的方法及其应用
WO2021108363A1 (en) 2019-11-25 2021-06-03 Regeneron Pharmaceuticals, Inc. Crispr/cas-mediated upregulation of humanized ttr allele
AU2020393880A1 (en) * 2019-11-27 2022-06-09 The General Hospital Corporation System and method for activating gene expression
EP4065701A4 (en) * 2019-11-27 2023-11-29 Danmarks Tekniske Universitet CONSTRUCTS, COMPOSITIONS AND METHODS THEREOF WITH IMPROVED GENOMEDITING EFFICIENCY AND SPECIFICITY
BR112022006229A2 (pt) 2019-11-29 2022-06-21 Basf Se Métodos para conferir ou aumentar a resistência a fungos, controlar um fungo e cultivar uma planta, construção de vetor recombinante, vetor de expressão recombinante, planta geneticamente modificada, planta de cultivo, métodos para a produção de uma planta e para a produção de um produto, uso de uma proteína, parte colhível de uma planta, produto derivado de uma planta e método de avaliação de uma planta
CN114787365A (zh) 2019-12-03 2022-07-22 巴斯夫欧洲公司 用于在植物中增强基因表达的调节性核酸分子
WO2021113522A1 (en) * 2019-12-04 2021-06-10 Arbor Biotechnologies, Inc. Compositions comprising a nuclease and uses thereof
WO2021113543A1 (en) 2019-12-06 2021-06-10 Precision Biosciences, Inc. Methods for cancer immunotherapy, using lymphodepletion regimens and cd19, cd20 or bcma allogeneic car t cells
WO2021113788A1 (en) * 2019-12-06 2021-06-10 Pairwise Plants Services, Inc. Recruitment methods and compounds, compositions and systems for recruitment
KR20250033331A (ko) 2019-12-10 2025-03-07 인스크립타 인코포레이티드 신규 mad 뉴클레아제
JP7749561B2 (ja) 2019-12-11 2025-10-06 アイオバンス バイオセラピューティクス，インコーポレイテッド 腫瘍浸潤リンパ球（ｔｉｌ）の産生のためのプロセス及びそれを使用する方法
US10704033B1 (en) 2019-12-13 2020-07-07 Inscripta, Inc. Nucleic acid-guided nucleases
RU2720767C1 (ru) * 2019-12-13 2020-05-13 Федеральное бюджетное учреждение науки «Центральный научно-исследовательский институт эпидемиологии» Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека (ФБУН ЦНИИ Эпидемиологии Роспотребнадзора) Способ обнаружения провирусной ДНК вируса иммунодефицита человека, интегрированной в геном человека, в ультранизких концентрациях и специфические олигонуклеотиды для использования в способе
RU2720769C1 (ru) * 2019-12-13 2020-05-13 Федеральное бюджетное учреждение науки «Центральный научно-исследовательский институт эпидемиологии» Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека (ФБУН ЦНИИ Эпидемиологии Роспотребнадзора) Способ получения препарата рибонуклеопротеинового комплекса CRISPR/CAS и препарат для детекции провирусной ДНК вируса иммунодефицита человека, интегрированной в геном человека, в ультранизких концентрациях
RU2720768C1 (ru) * 2019-12-13 2020-05-13 Федеральное бюджетное учреждение науки "Центральный научно-исследовательский институт эпидемиологии" Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека (ФБУН ЦНИИ Эпидемиологии Роспотребнадзора) Система CRISPR-Cas для детекции провирусной ДНК вируса иммунодефицита человека, интегрированной в геном человека, в ультранизких концентрациях
CN110791523B (zh) * 2019-12-13 2022-05-10 南京农业大学 一种棉花抗旱相关基因GhRCHY1及其应用
KR102493904B1 (ko) * 2019-12-13 2023-01-31 한국생명공학연구원 EeCpf1에 의해 IL2Rg 유전자가 돌연변이된 면역부전 동물모델 및 그 제조방법
CN114846144A (zh) 2019-12-16 2022-08-02 巴斯夫农业种子解决方案美国有限责任公司 将dna或突变精确引入小麦基因组
CN114829612A (zh) 2019-12-16 2022-07-29 巴斯夫农业种子解决方案美国有限责任公司 使用配对切口酶的改进的基因组编辑
EP4069851A4 (en) 2019-12-18 2023-11-22 Inscripta, Inc. Cascade/dcas3 complementation assays for in vivo detection of nucleic acid-guided nuclease edited cells
KR20220116504A (ko) 2019-12-19 2022-08-23 바스프 에스이 정밀 화학물의 제조에서 공시 수율, 탄소-전환-효율 및 탄소 기질 적응성의 증가
KR20220119408A (ko) 2019-12-20 2022-08-29 바스프 에스이 터펜의 독성 감소 및 미생물에서 생산 잠재성 증가
CA3163285A1 (en) 2019-12-30 2021-07-08 Alexandra Briner CRAWLEY Rna-guided nucleases and active fragments and variants thereof and methods of use
CN111073902B (zh) * 2019-12-30 2022-04-26 广东省微生物研究所（广东省微生物分析检测中心） 提升胶霉毒素生物合成基因表达水平的CRISPR/dCas9载体及其构建方法和应用
CN113073118B (zh) * 2020-01-03 2023-09-15 珠海联邦制药股份有限公司 Tet-off表达调控系统在级联放大启动子活性中的用途
CA3165756A1 (en) 2020-01-06 2021-07-15 Pairwise Plants Services, Inc. Recruitment of dna polymerase for templated editing
US10689669B1 (en) 2020-01-11 2020-06-23 Inscripta, Inc. Automated multi-module cell processing methods, instruments, and systems
WO2021146641A1 (en) 2020-01-17 2021-07-22 The Broad Institute, Inc. Small type ii-d cas proteins and methods of use thereof
EP4093187A1 (en) 2020-01-21 2022-11-30 Limagrain Europe Wheat haploid inducer plant and uses
WO2021151085A2 (en) 2020-01-24 2021-07-29 The General Hospital Corporation Crispr-cas enzymes with enhanced on-target activity
EP4093907A4 (en) * 2020-01-24 2024-01-17 The General Hospital Corporation METHODS FOR CHARACTERIZING ENZYMES FOR GENOMICS ENGINEERING
WO2021151073A2 (en) 2020-01-24 2021-07-29 The General Hospital Corporation Unconstrained genome targeting with near-pamless engineered crispr-cas9 variants
KR20220133257A (ko) 2020-01-27 2022-10-04 인스크립타 인코포레이티드 전기천공 모듈 및 기구
EP4097229A1 (en) 2020-01-30 2022-12-07 Pairwise Plants Services, Inc. Compositions, systems, and methods for base diversification
UY39058A (es) 2020-01-31 2021-08-31 Pairwise Plants Services Inc Suppresión de la respuesta de evasión a la sombra en plantas
US20230063560A1 (en) 2020-02-04 2023-03-02 Pairwise Plans Services, Inc. Thornless and/or prickleless rubus plants
CN111337666B (zh) * 2020-02-12 2021-04-02 山东大学 I-motif重组介导的FRET探针及其原位成像癌细胞表面蛋白质同源二聚化的应用
CN113261532A (zh) * 2020-02-17 2021-08-17 中山大学 一种肺癌动物模型的构建方法
EP4107273A1 (en) 2020-02-21 2022-12-28 Limagrain Europe Prime editing technology for plant genome engineering
MX2022010215A (es) 2020-02-21 2022-10-07 Pairwise Plants Services Inc Mejora de la resistencia al nematodo del quiste de la soja mediante edicion genetica.
EP4110817A1 (en) 2020-02-26 2023-01-04 Sorrento Therapeutics, Inc. Activatable antigen binding proteins with universal masking moieties
US12012433B1 (en) * 2020-02-27 2024-06-18 Integrated Dna Technologies Inc. Expression and purification of Cas enzymes
KR102647294B1 (ko) 2020-02-28 2024-03-13 후이진 테라퓨틱스 씨오., 엘티디. 유형 VI-E 및 유형 VI-F CRISPR-Cas 시스템 및 이의 용도
EP4110930A1 (en) 2020-02-28 2023-01-04 KWS SAAT SE & Co. KGaA Method for rapid genome modification in recalcitrant plants
CN115605600A (zh) 2020-02-28 2023-01-13 科沃施种子欧洲股份两合公司（De） 未成熟花序分生组织编辑
WO2021178556A1 (en) 2020-03-04 2021-09-10 Regeneron Pharmaceuticals, Inc. Methods and compositions for sensitization of tumor cells to immune therapy
MX2022011039A (es) * 2020-03-06 2022-12-13 Metagenomi Inc Sistemas crispr clase ii tipo v.
EP3878958A1 (en) 2020-03-11 2021-09-15 B.R.A.I.N. Biotechnology Research And Information Network AG Crispr-cas nucleases from cpr-enriched metagenome
JP2023517326A (ja) 2020-03-11 2023-04-25 オメガ セラピューティクス， インコーポレイテッド フォークヘッドボックスｐ３（ｆｏｘｐ３）遺伝子発現をモジュレートするための組成物および方法
US20220228150A1 (en) 2020-03-17 2022-07-21 Gowtham THAKKU Crispr system high throughput diagnostic systems and methods
EP4125348A1 (en) 2020-03-23 2023-02-08 Regeneron Pharmaceuticals, Inc. Non-human animals comprising a humanized ttr locus comprising a v30m mutation and methods of use
US11999946B2 (en) 2020-03-26 2024-06-04 Pairwise Plants Services, Inc. Methods for controlling meristem size for crop improvement
US11882808B2 (en) 2020-03-27 2024-01-30 Pairwise Plants Services, Inc. Methods for improving resistance to soybean rust
US20230263121A1 (en) 2020-03-31 2023-08-24 Elo Life Systems Modulation of endogenous mogroside pathway genes in watermelon and other cucurbits
WO2021207148A1 (en) 2020-04-06 2021-10-14 Pairwise Plants Services, Inc. Methods and compositions for increasing resistance to ear rot and stem rot disease in maize
EP4133069A2 (en) 2020-04-08 2023-02-15 Astrazeneca AB Compositions and methods for improved site-specific modification
RU2745637C1 (ru) * 2020-04-15 2021-03-29 Федеральное бюджетное учреждение науки "Центральный научно-исследовательский институт эпидемиологии" Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека (ФБУН ЦНИИ Эпидемиологии Роспотребнадзора) Способ получения препарата рибонуклеопротеинового комплекса CRISPR/Cas и препарат для выявления гена антибиотикоустойчивости bla VIM-2 (металло-бета-лактамаза класс B VIM-2) Pseudomonas aeruginosa в ультранизких концентрациях
RU2743861C1 (ru) * 2020-04-15 2021-03-01 Федеральное бюджетное учреждение науки "Центральный научно-исследовательский институт эпидемиологии" Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека (ФБУН ЦНИИ Эпидемиологии Роспотребнадзора) Система CRISPR-Cas для выявления гена антибиотикоустойчивости blaVIM-2 (металло-бета-лактамаза класс B VIM-2) Pseudomonas aeruginosa в ультранизких концентрациях
WO2021211926A1 (en) 2020-04-16 2021-10-21 Pairwise Plants Services, Inc. Methods for controlling meristem size for crop improvement
US20210332388A1 (en) 2020-04-24 2021-10-28 Inscripta, Inc. Compositions, methods, modules and instruments for automated nucleic acid-guided nuclease editing in mammalian cells
TW202208626A (zh) 2020-04-24 2022-03-01 美商生命編輯公司 Rna引導核酸酶及其活性片段與變體，以及使用方法
EP4139345A1 (en) 2020-04-24 2023-03-01 Sorrento Therapeutics, Inc. Memory dimeric antigen receptors
EP4143324A2 (en) 2020-05-01 2023-03-08 Integrated DNA Technologies, Inc. Lachnospiraceae sp cas12a mutants with enhanced cleavage activity at non-canonical tttt protospacer adjacent motifs
CA3176826A1 (en) 2020-05-04 2021-11-11 Iovance Biotherapeutics, Inc. Processes for production of tumor infiltrating lymphocytes and uses of the same in immunotherapy
BR112022022603A2 (pt) 2020-05-08 2023-01-17 Broad Inst Inc Métodos e composições para edição simultânea de ambas as fitas de sequência alvo de nucleotídeos de fita dupla
WO2021224506A1 (en) * 2020-05-08 2021-11-11 Universitat Pompeu Fabra Crispr-cas homology directed repair enhancer
US20230183664A1 (en) 2020-05-11 2023-06-15 Precision Biosciences, Inc. Self-limiting viral vectors encoding nucleases
EP4149243A1 (en) 2020-05-13 2023-03-22 Nunhems B.V. Method for obtaining mutant plants by targeted mutagenesis
US20230183737A1 (en) 2020-05-13 2023-06-15 Nunhems B.V. Tomato plants having suppressed meiotic recombination
US11787841B2 (en) 2020-05-19 2023-10-17 Inscripta, Inc. Rationally-designed mutations to the thrA gene for enhanced lysine production in E. coli
WO2021243283A1 (en) * 2020-05-28 2021-12-02 University Of Southern California Scalable trio guide rna approach for integration of large donor dna
JP2023527446A (ja) 2020-05-29 2023-06-28 カー・ヴェー・エス ザート エス・エー ウント コー． カー・ゲー・アー・アー 植物単数体の誘導
WO2021247477A1 (en) 2020-06-02 2021-12-09 Pairwise Plants Services, Inc. Methods for controlling meristem size for crop improvement
WO2021247924A1 (en) * 2020-06-03 2021-12-09 Mammoth Biosciences, Inc. Programmable nucleases and methods of use
CN111808832B (zh) * 2020-06-05 2022-02-11 华南农业大学 一种水稻纹枯病菌阳离子转运ATP酶基因及其片段Rscta和应用
JP7419168B2 (ja) * 2020-06-10 2024-01-22 株式会社東芝 改変型ｐｉｇｇｙＢａｃトランスポゼースのポリペプチド、それをコードするポリヌクレオチド、導入キャリア、キット、細胞のゲノムに目的配列を組込む方法及び細胞製造方法
CA3186580A1 (en) 2020-06-12 2021-12-16 Sherlock Biosciences, Inc. Crispr-based sars-cov-2 detection
CA3186972A1 (en) 2020-06-17 2021-12-23 Pairwise Plants Services, Inc. Methods for controlling meristem size for crop improvement
US20240216508A1 (en) 2020-06-26 2024-07-04 Juno Therapeutics Gmbh Engineered t cells conditionally expressing a recombinant receptor, related polynucleotides and methods
WO2022002989A1 (en) 2020-06-29 2022-01-06 KWS SAAT SE & Co. KGaA Boosting homology directed repair in plants
AU2021300169A1 (en) 2020-06-30 2023-02-02 Pairwise Plants Services, Inc. Compositions, systems, and methods for base diversification
EP4182454A1 (en) 2020-07-15 2023-05-24 Lifeedit Therapeutics, Inc. Uracil stabilizing proteins and active fragments and variants thereof and methods of use
CN111778252B (zh) * 2020-07-17 2022-01-07 湖北省农业科学院畜牧兽医研究所 一种靶向敲除SST基因的sgRNA及其CRISPR/Cas9系统和应用
EP4185699A1 (en) 2020-07-21 2023-05-31 Pairwise Plants Services, Inc. Optimized protein linkers and methods of use
EP3943600A1 (en) 2020-07-21 2022-01-26 B.R.A.I.N. Biotechnology Research And Information Network AG Novel, non-naturally occurring crispr-cas nucleases for genome editing
US11434525B2 (en) * 2020-08-06 2022-09-06 Singular Genomics Systems, Inc. Spatial sequencing
EP4192875A1 (en) 2020-08-10 2023-06-14 Precision BioSciences, Inc. Antibodies and fragments specific for b-cell maturation antigen and uses thereof
WO2022036180A1 (en) * 2020-08-13 2022-02-17 Yale University Compositions and methods for engineering and selection of car t cells with desired phenotypes
EP4199956A2 (en) * 2020-08-24 2023-06-28 Crisp-HR Therapeutics, Inc. A nuclease and application thereof
CN111979273B (zh) * 2020-08-24 2022-05-27 苏州启辰生物科技有限公司 一种制备人源化ace2小鼠模型的方法
KR20230058090A (ko) 2020-08-28 2023-05-02 페어와이즈 플랜츠 서비시즈, 인크. 조작된 crispr-cas 단백질 및 그의 사용 방법
EP4208545A4 (en) 2020-09-01 2024-10-30 The Board of Trustees of the Leland Stanford Junior University SYNTHETIC MINIATURE CRISPR-CAS SYSTEM (CASMINI) FOR EUKARYOTIC GENOME ENGINEERING
US20240052341A1 (en) 2020-09-01 2024-02-15 64-X, Inc. Mammalian cells and methods for engineering the same
CA3190991A1 (en) 2020-09-02 2022-03-10 Omar Abudayyeh Systems, methods, and compositions for rna-guided rna-targeting crispr effectors
IL301139A (en) 2020-09-11 2023-05-01 Lifeedit Therapeutics Inc Enzymes from secondary DNA and active fragments and their variants and methods of use
US11299731B1 (en) 2020-09-15 2022-04-12 Inscripta, Inc. CRISPR editing to embed nucleic acid landing pads into genomes of live cells
US20230323405A1 (en) 2020-09-18 2023-10-12 Artisan Development Labs, Inc. Constructs and uses thereof for efficient and specific genome editing
WO2022188039A1 (en) 2021-03-09 2022-09-15 Huigene Therapeutics Co., Ltd. Engineered crispr/cas13 system and uses thereof
WO2022068912A1 (en) 2020-09-30 2022-04-07 Huigene Therapeutics Co., Ltd. Engineered crispr/cas13 system and uses thereof
WO2022076606A1 (en) 2020-10-06 2022-04-14 Iovance Biotherapeutics, Inc. Treatment of nsclc patients with tumor infiltrating lymphocyte therapies
JP2023546359A (ja) 2020-10-06 2023-11-02 アイオバンス バイオセラピューティクス，インコーポレイテッド 腫瘍浸潤リンパ球療法によるｎｓｃｌｃ患者の治療
US20230365995A1 (en) 2020-10-07 2023-11-16 Precision Biosciences, Inc. Lipid nanoparticle compositions
CR20230175A (es) 2020-10-23 2023-07-26 Elo Life Systems Inc Métodos para producir plantas de vainilla con mejor sabor y producción agronómica
US20240026369A1 (en) 2020-10-27 2024-01-25 KWS SAAT SE & Co. KGaA Use of enhanced pol theta activity for eukaryotic genome engineering
CN112300999A (zh) * 2020-11-02 2021-02-02 江苏集萃药康生物科技股份有限公司 一种balb/c小鼠模型及其用途
US20230072431A1 (en) * 2020-11-03 2023-03-09 Science Solutions Llc Novel class 2 crispr-cas rna-guided endonucleases
WO2022097663A1 (ja) 2020-11-06 2022-05-12 エディットフォース株式会社 ＦｏｋＩヌクレアーゼドメインの変異体
AU2021374974A1 (en) 2020-11-06 2023-05-25 Pairwise Plants Services, Inc. Compositions and methods for rna-encoded dna-replacement of alleles
US11512297B2 (en) 2020-11-09 2022-11-29 Inscripta, Inc. Affinity tag for recombination protein recruitment
EP4244344A4 (en) * 2020-11-11 2024-10-16 The Trustees Of Columbia University In The City Of New York MULTIPLEX EPIGENOME EDITION
EP4243608A1 (en) 2020-11-11 2023-09-20 Leibniz-Institut für Pflanzenbiochemie (IPB) Fusion protein for editing endogenous dna of a eukaryotic cell
CN112410355B (zh) * 2020-11-23 2022-03-25 昆明理工大学 一种酰基辅酶a氧化酶2基因rkacox2及其应用
CN112461807B (zh) * 2020-11-26 2021-11-19 山西大学 碳量子点在靶向细胞核仁免洗成像中的应用
WO2022120022A1 (en) 2020-12-02 2022-06-09 Regeneron Pharmaceuticals, Inc. Crispr sam biosensor cell lines and methods of use thereof
PE20231178A1 (es) 2020-12-03 2023-08-01 Scribe Therapeutics Inc Sistemas crispr tipo v clase 2 disenados por ingenieria
CN113308451B (zh) * 2020-12-07 2023-07-25 中国科学院动物研究所 工程化的Cas效应蛋白及其使用方法
CN114634928B (zh) * 2020-12-15 2023-08-04 四川大学华西医院 一种降低stat3转录功能的核酸片段及其制药用途
US20240123067A1 (en) 2020-12-17 2024-04-18 Iovance Biotherapeutics, Inc. Treatment of cancers with tumor infiltrating lymphocyte therapies
US20240299539A1 (en) 2020-12-17 2024-09-12 Iovance Biotherapeutics, Inc. Treatment with tumor infiltrating lymphocyte therapies in combination with ctla-4 and pd-1 inhibitors
EP4263820A4 (en) * 2020-12-17 2025-03-26 Monsanto Technology LLC SSDNASE-FREE CRISPR ENDONUCLEASES
IL303892A (en) 2020-12-23 2023-08-01 Flagship Pioneering Innovations V Inc In vitro assembly of RNA-encapsulating enalavirus capsids
CN112553215A (zh) * 2020-12-24 2021-03-26 山东农业大学 一个玉米纹枯病抗病相关基因ZmFAH49及其应用
CN112662670B (zh) * 2020-12-25 2024-04-02 河南省农业科学院 花生脂酰-酰基载体蛋白硫酯酶AhFATB2基因启动子及其制备方法和应用
CN112640847B (zh) * 2020-12-30 2022-12-13 重庆医科大学附属第一医院 一种内源性癫痫发作动物模型及其构建方法
WO2022146497A1 (en) 2021-01-04 2022-07-07 Inscripta, Inc. Mad nucleases
WO2022150269A1 (en) 2021-01-07 2022-07-14 Inscripta, Inc. Mad nucleases
US20240318159A1 (en) 2021-01-12 2024-09-26 Mitolab Inc. Context-dependent, double-stranded dna-specific deaminases and uses thereof
CN112680479A (zh) * 2021-01-13 2021-04-20 汪利平 Cyp1b1基因缺失斑马鱼的制备
CN114790456A (zh) * 2021-01-24 2022-07-26 东北林业大学 野菊u6基因及其应用
JP2024509353A (ja) * 2021-01-25 2024-03-01 ザ・ブロード・インスティテュート・インコーポレイテッド 再プログラム可能なＴｎｐＢポリペプチド及びその使用
EP4284823A1 (en) 2021-01-28 2023-12-06 Precision BioSciences, Inc. Modulation of tgf beta signaling in genetically-modified eukaryotic cells
CA3206549A1 (en) 2021-01-29 2022-08-04 Frederick G. Vogt Methods of making modified tumor infiltrating lymphocytes and their use in adoptive cell therapy
CN112877275B (zh) * 2021-02-04 2023-03-31 中国农业科学院兰州兽医研究所 Hdac2基因敲除的bhk-21细胞系及其构建方法和应用
CN112852745B (zh) * 2021-02-04 2023-05-23 中国农业科学院兰州兽医研究所 Hdac3基因敲除的bhk-21细胞系及其构建方法和应用
WO2022166895A1 (zh) * 2021-02-05 2022-08-11 山东舜丰生物科技有限公司 Crispr酶和系统以及应用
IL305002A (en) * 2021-02-07 2023-10-01 Huidagene Therapeutics Singapore Pte Ltd Adeno-related virus vector (raav) and its uses
CA3210883A1 (en) 2021-02-09 2022-08-18 Sherlock Biosciences, Inc. Nucleic acid amplification using promoter primers
CA3210785A1 (en) 2021-02-11 2022-08-18 Pairwise Plants Services, Inc. Methods and compositions for modifying cytokinin oxidase levels in plants
US11884924B2 (en) 2021-02-16 2024-01-30 Inscripta, Inc. Dual strand nucleic acid-guided nickase editing
WO2022175878A1 (en) * 2021-02-20 2022-08-25 Kcat Enzymatic Private Limited Insilico guided crispr-cas driven enzyme engineering framework
EP4298118A1 (en) 2021-02-25 2024-01-03 Pairwise Plants Services, Inc. Methods and compositions for modifying root architecture in plants
CA3209863A1 (en) 2021-02-25 2022-09-01 Andrea BARGHETTI Compositions and methods for targeting, editing, or modifying genes
JP2024509139A (ja) 2021-03-02 2024-02-29 ブレイン バイオテック アーゲー メタゲノム由来の新規のｃｒｉｓｐｒ－ｃａｓヌクレアーゼ
WO2022187406A1 (en) 2021-03-03 2022-09-09 Juno Therapeutics, Inc. Combination of a t cell therapy and a dgk inhibitor
CA3211223A1 (en) * 2021-03-09 2022-09-15 Shaorong Chong Compositions comprising a variant polypeptide and uses thereof
CN115427561B (zh) 2021-03-09 2024-06-04 辉大(上海)生物科技有限公司 工程化CRISPR/Cas13系统及其用途
CN113005147A (zh) * 2021-03-11 2021-06-22 中山大学附属第一医院 一种usp8突变的小鼠动物模型的构建方法及其应用
TW202304480A (zh) 2021-03-19 2023-02-01 美商艾歐凡斯生物治療公司 腫瘤浸潤淋巴球（ｔｉｌ）中之與ｃｄ３９／ｃｄ６９選擇及基因剔除相關之ｔｉｌ擴增之方法
CA3173950A1 (en) 2021-03-22 2022-09-22 Tyson D. BOWEN Dna modifyng enzymes and active fragments and variants thereof and methods of use
JP2024513087A (ja) 2021-04-07 2024-03-21 アストラゼネカ・アクチエボラーグ 部位特異的改変のための組成物及び方法
US20240200059A1 (en) 2021-04-09 2024-06-20 Vor Biopharma Inc. Photocleavable guide rnas and methods of use thereof
KR20240007651A (ko) 2021-04-16 2024-01-16 빔 테라퓨틱스, 인크. 간세포의 유전적 변형
TW202308669A (zh) 2021-04-19 2023-03-01 美商艾歐凡斯生物治療公司 嵌合共刺激性受體、趨化激素受體及彼等於細胞免疫治療之用途
KR102428773B1 (ko) * 2021-04-21 2022-08-05 (주)지플러스생명과학 분리형 crRNA 및 이를 이용한 CRISPR-Cas 시스템
WO2022226316A1 (en) 2021-04-22 2022-10-27 Precision Biosciences, Inc. Compositions and methods for generating male sterile plants
JP2024518413A (ja) 2021-05-06 2024-05-01 アーティザン ディヴェロップメント ラブス インコーポレイテッド 修飾ヌクレアーゼ
US20240269180A1 (en) 2021-05-17 2024-08-15 Iovance Biotherapeutics, Inc. Pd-1 gene-edited tumor infiltrating lymphocytes and uses of same in immunotherapy
WO2022243437A1 (en) 2021-05-19 2022-11-24 KWS SAAT SE & Co. KGaA Sample preparation with oppositely oriented guide polynucleotides
CN113287569B (zh) * 2021-05-27 2023-04-07 四川康城生物科技有限公司 一种免疫低下动物模型的构建方法
US20220389418A1 (en) 2021-06-01 2022-12-08 Helmholtz-Institut Für Rna-Basierte Nfektionsforschung (Hzi) Rna-guided cas nucleases and uses thereof in diagnostics and therapy
WO2022256448A2 (en) 2021-06-01 2022-12-08 Artisan Development Labs, Inc. Compositions and methods for targeting, editing, or modifying genes
WO2022256440A2 (en) 2021-06-01 2022-12-08 Arbor Biotechnologies, Inc. Gene editing systems comprising a crispr nuclease and uses thereof
CN113064220A (zh) * 2021-06-03 2021-07-02 四川九通智路科技有限公司 基于非线性自回归神经网络的能见度测量系统及测量方法
CA3173953A1 (en) 2021-06-11 2023-12-10 Tyson D. BOWEN Rna polymerase iii promoters and methods of use
US20220403475A1 (en) 2021-06-14 2022-12-22 Pairwise Plants Services, Inc. Reporter constructs, compositions comprising the same, and methods of use thereof
US20220411813A1 (en) 2021-06-17 2022-12-29 Pairwise Plants Services, Inc. Modification of growth regulating factor family transcription factors in soybean
WO2022266439A2 (en) * 2021-06-17 2022-12-22 The Board Of Trustees Of The University Of Illinois Crispr cascade
EP4370676A2 (en) 2021-06-18 2024-05-22 Artisan Development Labs, Inc. Compositions and methods for targeting, editing or modifying human genes
UY39827A (es) 2021-06-24 2023-01-31 Pairwise Plants Services Inc Modificación de genes de ubiquitina ligasa e3 hect para mejorar los rasgos de rendimiento
CA3224982A1 (en) 2021-07-01 2023-01-05 Pairwise Plants Services, Inc. Methods and compositions for enhancing root system development
EP4368713A4 (en) * 2021-07-05 2025-10-08 Genkore Inc CLEAVAGE-INACTIVE CAS12F1, CLEAVAGE-INACTIVE CAS12F1-BASED FUSION PROTEIN, CRISPR GENE EDITING SYSTEM COMPRISING SAME, PREPARATION METHOD AND USE THEREOF
WO2023283585A2 (en) 2021-07-06 2023-01-12 Vor Biopharma Inc. Inhibitor oligonucleotides and methods of use thereof
CN117987554A (zh) * 2021-07-06 2024-05-07 苏州淦江生物技术有限公司 一种基于Cas蛋白的检测方法
EP4367242A2 (en) 2021-07-07 2024-05-15 Omega Therapeutics, Inc. Compositions and methods for modulating secreted frizzled receptor protein 1 (sfrp1) gene expression
WO2023283636A1 (en) * 2021-07-09 2023-01-12 Acrigen Biosciences Compositions and methods for nucleic acid modifications
CN113383747B (zh) * 2021-07-09 2022-12-27 广西贺州农贝贝农牧科技有限公司 一种产蛋后期老龄鸡强制换羽的工艺方法
US11639520B2 (en) 2021-07-12 2023-05-02 Labsimply, Inc. Compositions of matter for detection assays
CA3226111A1 (en) 2021-07-22 2023-01-26 Iovance Biotherapeutics, Inc. Method for cryopreservation of solid tumor fragments
CA3226942A1 (en) 2021-07-28 2023-02-02 Iovance Biotherapeutics, Inc. Treatment of cancer patients with tumor infiltrating lymphocyte therapies in combination with kras inhibitors
AR126622A1 (es) 2021-07-30 2023-10-25 Kws Saat Se & Co Kgaa Plantas con digestibilidad mejorada y haplotipos marcadores
EP4381062A1 (en) 2021-08-02 2024-06-12 Vor Biopharma Inc. Compositions and methods for gene modification
EP4384626A1 (en) 2021-08-12 2024-06-19 Pairwise Plants Services, Inc. Modification of brassinosteroid receptor genes to improve yield traits
WO2023020938A1 (en) 2021-08-16 2023-02-23 Nunhems B.V. Lettuce plant having delayed bolting
UY39902A (es) 2021-08-17 2023-03-31 Pairwise Plants Services Inc Métodos y composiciones para modificar genes de histidina quinasa receptores de citoquinina en plant
WO2023028444A1 (en) * 2021-08-27 2023-03-02 Mammoth Biosciences, Inc. Effector proteins and methods of use
US12359215B2 (en) 2021-08-30 2025-07-15 Pairwise Plants Services, Inc. Modification of ubiquitin binding peptidase genes in plants for yield trait improvement
CN113584077B (zh) * 2021-08-31 2023-09-15 西南大学 沉默桑实杯盘菌关键致病基因CsGPA1的沉默载体及其应用和方法
AR126938A1 (es) 2021-09-02 2023-11-29 Pairwise Plants Services Inc Métodos y composiciones para mejorar la arquitectura de las plantas y los rasgos de rendimiento
CA3232804A1 (en) 2021-09-21 2023-03-30 Pairwise Plants Services, Inc. Methods and compositions for reducing pod shatter in canola
US20230266293A1 (en) 2021-09-21 2023-08-24 Pairwise Plants Services, Inc. Color-based and/or visual methods for identifying the presence of a transgene and compositions and constructs relating to the same
JP2024535920A (ja) 2021-09-27 2024-10-02 ブイオーアール バイオファーマ インコーポレーテッド 遺伝子編集のための融合ポリペプチド及びその使用方法
EP4409007A4 (en) 2021-09-27 2025-10-08 Monsanto Technology Llc COMPOSITIONS AND METHODS FOR TRANSFORMING EXPLANTS OF EXCISED EMBRYO FROM MONOCOTYLEDONOUS SEEDS
CN118119707A (zh) 2021-09-30 2024-05-31 阿斯利康(瑞典)有限公司 抑制剂增加CRISPR/Cas插入效率的用途
MX2024004057A (es) 2021-10-04 2024-04-25 Pairwise Plants Services Inc Metodos para mejorar la fertilidad de la flor y el rendimiento de semillas.
KR102703683B1 (ko) * 2021-10-06 2024-09-09 주식회사 진코어 유전자 편집을 위한 TaRGET 시스템 및 이의 용도
EP4413022A2 (en) 2021-10-07 2024-08-14 Pairwise Plants Services, Inc. Methods for improving floret fertility and seed yield
WO2023064872A1 (en) 2021-10-14 2023-04-20 Precision Biosciences, Inc. Combinations of anti-bcma car t cells and gamma secretase inhibitors
EP4166670A1 (en) 2021-10-18 2023-04-19 KWS SAAT SE & Co. KGaA Plant-tag-based weeding control
WO2023070003A1 (en) 2021-10-19 2023-04-27 Precision Biosciences, Inc. Gene editing methods for treating alpha-1 antitrypsin (aat) deficiency
CN116024254A (zh) * 2021-10-26 2023-04-28 成都极谷基因科技有限公司 编辑水稻基因组中OsGBSSⅠ启动子及内含子1以提高稻米品质的方法
TW202331735A (zh) 2021-10-27 2023-08-01 美商艾歐凡斯生物治療公司 協調用於患者特異性免疫療法之細胞之製造的系統及方法
US20240415980A1 (en) 2021-10-28 2024-12-19 Regeneron Pharmaceuticals, Inc. Crispr/cas-related methods and compositions for knocking out c5
AU2022375820A1 (en) 2021-11-01 2024-06-13 Tome Biosciences, Inc. Single construct platform for simultaneous delivery of gene editing machinery and nucleic acid cargo
WO2023081900A1 (en) 2021-11-08 2023-05-11 Juno Therapeutics, Inc. Engineered t cells expressing a recombinant t cell receptor (tcr) and related systems and methods
JP2024544867A (ja) 2021-11-10 2024-12-05 アイオバンス バイオセラピューティクス，インコーポレイテッド Ｃｄ８腫瘍浸潤リンパ球を利用する拡張治療の方法
US20250009800A1 (en) 2021-11-16 2025-01-09 Precision Biosciences, Inc. Methods for cancer immunotherapy
US20250025572A1 (en) * 2021-11-29 2025-01-23 Editas Medicine, Inc. Engineered crispr/cas12a effector proteins, and uses thereof
CN114404429B (zh) * 2021-11-30 2023-06-30 重庆医科大学附属第二医院 一种纳米银修饰的单宁酸-铁网络的载药纳米复合物及其制备方法和逆转肿瘤耐药应用
AU2022408167A1 (en) 2021-12-08 2024-06-06 Regeneron Pharmaceuticals, Inc. Mutant myocilin disease model and uses thereof
AR127904A1 (es) 2021-12-09 2024-03-06 Pairwise Plants Services Inc Métodos para mejorar la fertilidad de floretes y el rendimiento de semillas
US11820983B2 (en) 2021-12-13 2023-11-21 Labsimply, Inc. Tuning cascade assay kinetics via molecular design
US20230279375A1 (en) 2021-12-13 2023-09-07 Labsimply, Inc. Signal boost cascade assay
WO2023114750A1 (en) 2021-12-13 2023-06-22 Pairwise Plants Services, Inc. Model editing systems and methods relating to the same
US20250304982A1 (en) 2021-12-21 2025-10-02 Benson Hill, Inc. Compositions and methods for modifying genomes
IL313765A (en) 2021-12-22 2024-08-01 Tome Biosciences Inc Joint provision of a gene editor structure and a donor template
EP4453212A1 (en) 2021-12-23 2024-10-30 University of Massachusetts Therapeutic treatment for fragile x-associated disorder
WO2023133440A1 (en) 2022-01-06 2023-07-13 Pairwise Plants Services, Inc. Methods and compositions for trichome removal
JP2025505063A (ja) * 2022-01-12 2025-02-20 ジーンシーカー カンパニー， リミテッド 増強された特異性を有するｃａｓ９タンパク質及びその使用
WO2023140695A1 (ko) * 2022-01-21 2023-07-27 한국과학기술연구원 우유 유래 엑소좀을 유효성분으로 포함하는 장 건강기능 개선용 조성물 및 그 제조방법
AU2023208961A1 (en) 2022-01-24 2024-09-12 Life Edit Therapeutics, Inc. Rna-guided nucleases and active fragments and variants thereof and methods of use
WO2023147488A1 (en) 2022-01-28 2023-08-03 Iovance Biotherapeutics, Inc. Cytokine associated tumor infiltrating lymphocytes compositions and methods
AR128372A1 (es) 2022-01-31 2024-04-24 Pairwise Plants Services Inc Supresión de la respuesta de evitación de la sombra en las plantas
US20230338477A1 (en) 2022-02-02 2023-10-26 Regeneron Pharmaceuticals, Inc. Anti-tfr:gaa and anti-cd63:gaa insertion for treatment of pompe disease
WO2023154887A1 (en) 2022-02-11 2023-08-17 Northeast Agricultural University Methods and compositions for increasing protein and/or oil content and modifying oil profile in a plant
JP2025508723A (ja) * 2022-02-18 2025-04-10 プルモバイオティクス，エス．エル． 細菌を操作するためのシステム、ツール、及び方法
CA3253290A1 (en) 2022-02-28 2023-08-31 Pairwise Plants Services, Inc. MODIFIED CRISPR-CAS EFFECTOR PROTEINS AND THEIR METHODS OF USE
KR20240152394A (ko) 2022-03-01 2024-10-21 에피크리스피알 바이오테크놀로지스, 인크. 조작된 뉴클레아제, 그의 조성물 및 사용 방법
WO2023167882A1 (en) 2022-03-01 2023-09-07 Artisan Development Labs, Inc. Composition and methods for transgene insertion
CN119072487A (zh) 2022-03-02 2024-12-03 成对植物服务股份有限公司 用于改善产量性状的油菜素类固醇受体基因的修饰
CN114271237B (zh) * 2022-03-07 2022-05-27 南京农业大学 一种长短绒自别雌雄鸽的培育方法
US20250263684A1 (en) 2022-03-08 2025-08-21 Institute Of Genetics And Developmental Biology, Chinese Academy Of Sciences Cytosine deaminase and use thereof in base editing
CN114592023B (zh) * 2022-03-31 2023-03-24 杭州优玛达生物科技有限公司 一种细胞裂解自组装多肽复合物、自组装方法、自组装多肽制剂及应用
WO2023192838A1 (en) 2022-03-31 2023-10-05 Pairwise Plants Services, Inc. Early flowering rosaceae plants with improved characteristics
WO2023196877A1 (en) 2022-04-06 2023-10-12 Iovance Biotherapeutics, Inc. Treatment of nsclc patients with tumor infiltrating lymphocyte therapies
CA3247405A1 (en) 2022-04-07 2023-10-12 Pairwise Plants Services, Inc. METHODS AND COMPOSITIONS FOR IMPROVING RESISTANCE TO FUSARIA BROAD OF THE SPINE
WO2023201369A1 (en) 2022-04-15 2023-10-19 Iovance Biotherapeutics, Inc. Til expansion processes using specific cytokine combinations and/or akti treatment
WO2023205744A1 (en) 2022-04-20 2023-10-26 Tome Biosciences, Inc. Programmable gene insertion compositions
WO2023205714A1 (en) 2022-04-21 2023-10-26 Pairwise Plants Services, Inc. Methods and compositions for improving yield traits
EP4514981A2 (en) 2022-04-29 2025-03-05 Regeneron Pharmaceuticals, Inc. Identification of tissue-specific extragenic safe harbors for gene therapy approaches
WO2023215704A1 (en) 2022-05-02 2023-11-09 Pairwise Plants Services, Inc. Methods and compositions for enhancing yield and disease resistance
WO2023215831A1 (en) 2022-05-04 2023-11-09 Tome Biosciences, Inc. Guide rna compositions for programmable gene insertion
WO2023215809A1 (en) 2022-05-05 2023-11-09 Pairwise Plants Services, Inc. Methods and compositions for modifying root architecture and/or improving plant yield traits
JP2025516527A (ja) 2022-05-09 2025-05-30 リジェネロン・ファーマシューティカルズ・インコーポレイテッド インビボ抗体産生のためのベクター及び方法
EP4522202A1 (en) 2022-05-10 2025-03-19 Iovance Biotherapeutics, Inc. Treatment of cancer patients with tumor infiltrating lymphocyte therapies in combination with an il-15r agonist
US20250308637A1 (en) 2022-05-20 2025-10-02 Celyntra Therapeutics Sa Systems and methods for assessing risk of genome editing events
WO2023225670A2 (en) 2022-05-20 2023-11-23 Tome Biosciences, Inc. Ex vivo programmable gene insertion
KR20250017230A (ko) 2022-05-25 2025-02-04 플래그쉽 파이어니어링 이노베이션스 Vii, 엘엘씨 사이토카인을 조절하기 위한 조성물 및 방법
AU2023276715A1 (en) 2022-05-25 2025-01-09 Flagship Pioneering Innovations Vii, Llc Compositions and methods for modulating circulating factors
CN119836571A (zh) 2022-05-25 2025-04-15 旗舰创业创新第七有限责任公司 用于调节遗传驱动因子的组合物和方法
CN114934031B (zh) 2022-05-25 2023-08-01 广州瑞风生物科技有限公司 新型Cas效应蛋白、基因编辑系统及用途
WO2023230498A1 (en) 2022-05-25 2023-11-30 Massachusetts Institute Of Technology Systems, methods, and compositions for rna-guided rna-targeting crispr effectors with cas7-11 variants
EP4532765A2 (en) 2022-05-25 2025-04-09 Flagship Pioneering Innovations VII, LLC Compositions and methods for modulation of immune responses
EP4532771A2 (en) 2022-05-25 2025-04-09 Flagship Pioneering Innovations VII, LLC Compositions and methods for modulation of tumor suppressors and oncogenes
EP4532720A2 (en) 2022-05-31 2025-04-09 Regeneron Pharmaceuticals, Inc. Crispr interference therapeutics for c9orf72 repeat expansion disease
WO2023235725A2 (en) 2022-05-31 2023-12-07 Regeneron Pharmaceuticals, Inc. Crispr-based therapeutics for c9orf72 repeat expansion disease
AR129505A1 (es) 2022-06-01 2024-09-04 Inst Genetics & Developmental Biology Cas Novedoso sistema de edición de genes crispr
CN119731321A (zh) 2022-06-24 2025-03-28 图恩疗法股份有限公司 通过靶向基因阻遏减少低密度脂蛋白的组合物、系统和方法
UY40326A (es) 2022-06-27 2023-12-29 Pairwise Plants Services Inc Métodos y composiciones para modificar el escape a la sombra en plantas
CN119365481A (zh) 2022-06-29 2025-01-24 成对植物服务股份有限公司 用于控制分生组织大小以进行作物改良的方法和组合物
CN119487055A (zh) 2022-06-29 2025-02-18 成对植物服务股份有限公司 用于控制分生组织大小以进行作物改良的方法和组合物
EP4299733A1 (en) 2022-06-30 2024-01-03 Inari Agriculture Technology, Inc. Compositions, systems, and methods for genome editing
EP4299739A1 (en) 2022-06-30 2024-01-03 Inari Agriculture Technology, Inc. Compositions, systems, and methods for genome editing
WO2024005864A1 (en) 2022-06-30 2024-01-04 Inari Agriculture Technology, Inc. Compositions, systems, and methods for genome editing
WO2024005863A1 (en) 2022-06-30 2024-01-04 Inari Agriculture Technology, Inc. Compositions, systems, and methods for genome editing
WO2024020587A2 (en) 2022-07-22 2024-01-25 Tome Biosciences, Inc. Pleiopluripotent stem cell programmable gene insertion
KR20250054842A (ko) 2022-07-29 2025-04-23 리제너론 파마슈티칼스 인코포레이티드 뇌 및 근육으로의 트랜스페린 수용체(tfr)-매개 전달을 위한 조성물 및 방법
WO2024030984A1 (en) 2022-08-04 2024-02-08 Pairwise Plants Services, Inc. Methods and compositions for improving yield traits
CN119654065A (zh) 2022-08-11 2025-03-18 成对植物服务股份有限公司 用于控制分生组织大小以进行作物改良的方法和组合物
CN120112633A (zh) 2022-08-12 2025-06-06 生命编辑治疗股份有限公司 Rna引导的核酸酶及其活性片段和变体以及使用方法
WO2024038168A1 (en) 2022-08-19 2024-02-22 UCB Biopharma SRL Novel rna-guided nucleases and nucleic acid targeting systems comprising such
WO2024044717A1 (en) 2022-08-25 2024-02-29 Massachusetts Institute Of Technology Programmable rna writing using crispr effectors and trans-splicing templates
TW202424186A (zh) 2022-08-25 2024-06-16 美商生命編輯治療學公司 Rna引導核酸酶中介基因編輯用之具鎖核酸引導 rna之化學修飾
AU2023329149A1 (en) 2022-08-26 2025-02-20 KWS SAAT SE & Co. KGaA Use of paired genes in hybrid breeding
WO2024042168A1 (en) 2022-08-26 2024-02-29 UCB Biopharma SRL Novel rna-guided nucleases and nucleic acid targeting systems comprising such rna-guided nucleases
WO2024042165A2 (en) 2022-08-26 2024-02-29 UCB Biopharma SRL Novel rna-guided nucleases and nucleic acid targeting systems comprising such rna-guided nucleases
WO2024050548A2 (en) * 2022-09-02 2024-03-07 Hunterian Medicine Llc Compact promoters for targeting hypoxia induced genes
EP4580663A1 (en) 2022-09-02 2025-07-09 Janssen Biotech, Inc. Materials and processes for engineering hypoimmunogenicity
CA3266121A1 (en) 2022-09-08 2024-03-14 Pairwise Plants Services, Inc. METHODS AND COMPOSITIONS FOR IMPROVING YIELD CHARACTERISTICS IN PLANTS
JP2025531850A (ja) 2022-09-08 2025-09-25 ジュノー セラピューティクス インコーポレイテッド Ｔ細胞療法および継続的または間欠的ｄｇｋ阻害剤投薬の組合せ
WO2024062138A1 (en) 2022-09-23 2024-03-28 Mnemo Therapeutics Immune cells comprising a modified suv39h1 gene
KR20250075694A (ko) 2022-09-28 2025-05-28 리제너론 파마슈티칼스 인코포레이티드 세포 기반 요법을 강화하기 위한 항체 저항성 변형 수용체
WO2024073751A1 (en) 2022-09-29 2024-04-04 Vor Biopharma Inc. Methods and compositions for gene modification and enrichment
WO2024073114A1 (en) 2022-09-30 2024-04-04 Vedabio, Inc. Delivery of therapeutics in vivo via a crispr-based cascade system
US11982677B2 (en) 2022-10-02 2024-05-14 Vedabio, Inc. Dimerization screening assays
US11965205B1 (en) 2022-10-14 2024-04-23 Vedabio, Inc. Detection of nucleic acid and non-nucleic acid target molecules
EP4605524A1 (en) 2022-10-20 2025-08-27 Basf Se Regulatory nucleic acid molecules for enhancing gene expression in plants
WO2024098027A1 (en) 2022-11-04 2024-05-10 Iovance Biotherapeutics, Inc. Methods for tumor infiltrating lymphocyte (til) expansion related to cd39/cd103 selection
CN120693347A (zh) 2022-11-04 2025-09-23 瑞泽恩制药公司 钙电压门控通道辅助亚基γ1(CACNG1)结合蛋白和CACNG1介导的向骨骼肌的递送
TW202434726A (zh) 2022-11-04 2024-09-01 美商生命編輯治療學公司 具內部插入位進化腺嘌呤去胺及rna引導之核酸酶融合蛋白及其使用方法
WO2024098024A1 (en) 2022-11-04 2024-05-10 Iovance Biotherapeutics, Inc. Expansion of tumor infiltrating lymphocytes from liquid tumors and therapeutic uses thereof
CN120239706A (zh) 2022-11-07 2025-07-01 先正达农作物保护股份公司 改变大豆植物开花时间和/或成熟的基因及其用途
WO2024100604A1 (en) 2022-11-09 2024-05-16 Juno Therapeutics Gmbh Methods for manufacturing engineered immune cells
CN120359023A (zh) 2022-11-10 2025-07-22 赛欧生物医药股份有限公司 包含脂质纳米颗粒或脂质重构的天然信使包的rna组合物
WO2024107765A2 (en) 2022-11-14 2024-05-23 Regeneron Pharmaceuticals, Inc. Compositions and methods for fibroblast growth factor receptor 3-mediated delivery to astrocytes
IL320733A (en) 2022-11-21 2025-07-01 Iovance Biotherapeutics Inc Two-dimensional processes for expanding tumor-infiltrating lymphocytes and treatments thereof
WO2024118836A1 (en) 2022-11-30 2024-06-06 Iovance Biotherapeutics, Inc. Processes for production of tumor infiltrating lymphocytes with shortened rep step
AU2023395894A1 (en) 2022-12-16 2025-06-05 Pairwise Plants Services, Inc. Fusion proteins comprising an intein polypeptide and methods of use thereof
CA3277009A1 (en) 2022-12-16 2024-06-20 Life Edit Therapeutics, Inc. RNA GUIDELINES TARGETING THE TRAC GENE AND METHODS OF USE
WO2024127369A1 (en) 2022-12-16 2024-06-20 LifeEDIT Therapeutics, Inc. Guide rnas that target foxp3 gene and methods of use
WO2024137911A1 (en) 2022-12-21 2024-06-27 Pairwise Plants Services, Inc. Engineered proteins and methods of use thereof
WO2024138194A1 (en) 2022-12-22 2024-06-27 Tome Biosciences, Inc. Platforms, compositions, and methods for in vivo programmable gene insertion
CN118275664A (zh) * 2022-12-30 2024-07-02 上海吐露港生物科技有限公司 基于拆分Cas蛋白的非核酸靶标检测方法及应用
WO2024253707A2 (en) 2023-01-07 2024-12-12 Vedabio, Inc. Engineered nucleic acid-guided nucleases
US12060602B2 (en) 2023-01-10 2024-08-13 Vedabio, Inc. Sample splitting for multiplexed detection of nucleic acids without amplification
CN121002052A (zh) 2023-02-03 2025-11-21 C3S2 有限公司 用于非病毒生产工程化免疫细胞的方法
WO2024167317A1 (ko) * 2023-02-10 2024-08-15 주식회사 엔세이지 신규 CRISPR/Cas12a 조성물 및 이의 표적 핵산 검출 용도
WO2024173645A1 (en) 2023-02-15 2024-08-22 Arbor Biotechnologies, Inc. Gene editing method for inhibiting aberrant splicing in stathmin 2 (stmn2) transcript
EP4665747A1 (en) 2023-02-16 2025-12-24 Pairwise Plants Services, Inc. Methods and compositions for modifying shade avoidance in plants
WO2024175308A1 (en) 2023-02-22 2024-08-29 KWS SAAT SE & Co. KGaA Plants with improved performance
CN116355878B (zh) * 2023-02-28 2024-04-26 华中农业大学 新型TnpB编程性核酸酶及其应用
WO2024182746A1 (en) 2023-03-01 2024-09-06 Pairwise Plants Services, Inc. Engineered proteins and methods of use thereof
AR132019A1 (es) 2023-03-02 2025-05-21 Pairwise Plants Services Inc Métodos y composiciones para modificar la evitación de la sombra en plantas
AR132071A1 (es) 2023-03-09 2025-05-21 Pairwise Plants Services Inc Modificación de genes de la vía de señalización de brasinoesteroide para mejorar rasgos de rendimiento en plantas
AU2024236558A1 (en) 2023-03-15 2025-10-09 Renagade Therapeutics Management Inc. Delivery of gene editing systems and methods of use thereof
WO2024201368A1 (en) 2023-03-29 2024-10-03 Astrazeneca Ab Use of inhibitors to increase efficiency of crispr/cas insertions
AU2024248777A1 (en) 2023-03-30 2025-10-02 Pairwise Plants Services, Inc. Methods and compositions for reducing thorns or prickles in plants
WO2024211306A1 (en) 2023-04-03 2024-10-10 Sutro Biopharma, Inc. Rf1 ko e. coli strains
WO2024211287A1 (en) 2023-04-03 2024-10-10 Seagen Inc. Production cell lines with targeted integration sites
WO2024216116A1 (en) 2023-04-14 2024-10-17 Precision Biosciences, Inc. Muscle-specific expression cassettes
WO2024216118A1 (en) 2023-04-14 2024-10-17 Precision Biosciences, Inc. Muscle-specific expression cassettes
WO2024218295A1 (en) 2023-04-21 2024-10-24 Vib Vzw Allelic combinations in crops for yield increase
CN116751763B (zh) * 2023-05-08 2024-02-13 珠海舒桐医疗科技有限公司 一种Cpf1蛋白、V型基因编辑系统及应用
WO2024234006A1 (en) 2023-05-11 2024-11-14 Tome Biosciences, Inc. Systems, compositions, and methods for targeting liver sinusodial endothelial cells (lsecs)
WO2024235991A1 (en) 2023-05-15 2024-11-21 UCB Biopharma SRL Rna-guided nucleases and nucleic acid targeting systems comprising such rna-guided nucleases
UY40746A (es) 2023-05-18 2024-12-13 Pairwise Plants Services Inc Métodos y composiciones para mejorar las características de rendimiento de las plantas
WO2024236547A1 (en) 2023-05-18 2024-11-21 Inceptor Bio, Llc Modified phagocytic cells expressing chimeric antigen receptors comprising a herpes virus entry mediator (hvem) co-stimulatory domain and uses thereof
WO2024243438A2 (en) 2023-05-23 2024-11-28 Omega Therapeutics, Inc. Compositions and methods for reducing cxcl9, cxcl10, and cxcl11 gene expression
WO2025003358A2 (en) 2023-06-29 2025-01-02 UCB Biopharma SRL Novel nucleic acid targeting systems comprising rna-guided nucleases
US20250002946A1 (en) 2023-06-30 2025-01-02 Regeneron Pharmaceuticals, Inc. Methods And Compositions For Increasing Homology-Directed Repair
CN116671491B (zh) * 2023-07-04 2024-01-23 四川省畜牧科学研究院 一种南江黄羊的种羊选育方法
US12291729B2 (en) 2023-07-07 2025-05-06 Shandong Shunfeng Biotechnology Co.Ltd. Engineered Cas protein and use thereof
CN116790559B (zh) * 2023-07-07 2023-11-28 山东舜丰生物科技有限公司 一种融合HNH结构域的V型Cas酶及其应用
AR133164A1 (es) 2023-07-18 2025-09-03 Pairwise Plants Services Inc Métodos y composiciones para modificar la arquitectura radicular en plantas
AR133310A1 (es) 2023-07-27 2025-09-17 Pairwise Plants Services Inc Métodos y composiciones para modificar rasgos de rendimiento de las plantas
WO2025022367A2 (en) 2023-07-27 2025-01-30 Life Edit Therapeutics, Inc. Rna-guided nucleases and active fragments and variants thereof and methods of use
AR133385A1 (es) 2023-07-28 2025-09-24 Regeneron Pharma Inserción anti-tfr:gaa y anti-cd63:gaa para el tratamiento de la enfermedad de pompe
WO2025029654A2 (en) 2023-07-28 2025-02-06 Regeneron Pharmaceuticals, Inc. Use of bgh-sv40l tandem polya to enhance transgene expression during unidirectional gene insertion
WO2025029662A1 (en) 2023-07-28 2025-02-06 Regeneron Pharmaceuticals, Inc. Anti-tfr: acid sphingomyelinase for treatment of acid sphingomyelinase deficiency
WO2025027166A1 (en) 2023-08-01 2025-02-06 Basf Plant Science Company Gmbh Increased resistance by expression of msbp1 protein
AR133438A1 (es) 2023-08-01 2025-10-01 Kws Saat Se & Co Kgaa Método para mejorar las propiedades de gelificación de proteínas alternativas
AR133439A1 (es) 2023-08-01 2025-10-01 Kws Saat Se & Co Kgaa Método para reducir los sabores desagradables en proteínas alternativas
WO2025027165A1 (en) 2023-08-01 2025-02-06 Basf Plant Science Company Gmbh Increased resistance by expression of an ics protein
WO2025049524A1 (en) 2023-08-28 2025-03-06 Regeneron Pharmaceuticals, Inc. Cxcr4 antibody-resistant modified receptors
WO2025046513A1 (en) 2023-08-29 2025-03-06 Inceptor Bio, Llc Methods of manufacturing myeloid-derived cells from hematopoietic stem cells and compositions and uses thereof
WO2025049959A2 (en) 2023-09-01 2025-03-06 Renagade Therapeutics Management Inc. Gene editing systems, compositions, and methods for treatment of vexas syndrome
WO2025050069A1 (en) 2023-09-01 2025-03-06 Tome Biosciences, Inc. Programmable gene insertion using engineered integration enzymes
WO2025054540A1 (en) 2023-09-08 2025-03-13 Iovance Biotherapeutics, Inc. Methods of gene-editing using programmable nucleases
KR102789465B1 (ko) * 2023-09-20 2025-04-04 주식회사 엔세이지 신규 CRISPR/Cas12a 시스템 및 그 유전자 편집 용도
WO2025072430A1 (en) 2023-09-26 2025-04-03 Pairwise Plants Services, Inc. Crispr-cas fusion proteins with adenine base editor functionality, compositions comprising the same, and methods of use thereof
EP4554371A1 (en) 2023-10-03 2025-05-21 Inari Agriculture Technology, Inc. Viral delivery of grna to the scion
WO2025080537A1 (en) 2023-10-09 2025-04-17 Pairwise Plants Services, Inc. Circular permutants of cas12a
US20250122522A1 (en) 2023-10-11 2025-04-17 Pairwise Plants Services, Inc. Methods and compositions for improving crop yield traits
WO2025101484A1 (en) 2023-11-06 2025-05-15 Iovance Biotherapeutics, Inc. Treatment of endometrial cancers with tumor infiltrating lymphocyte therapies
EP4560016A1 (en) * 2023-11-22 2025-05-28 Plantik Biosciences Method of plant genome editing
CN117286039B (zh) * 2023-11-27 2024-03-12 中国科学院天津工业生物技术研究所 一种菌丝形态优化的里氏木霉菌株及其应用
US20250207154A1 (en) 2023-12-21 2025-06-26 Pairwise Plants Services, Inc. Compositions and methods for rna-encoded dna-replacement of alleles
WO2025132866A1 (en) 2023-12-21 2025-06-26 KWS SAAT SE & Co. KGaA Maize plants with improved disease resistance
WO2025153582A1 (en) 2024-01-17 2025-07-24 KWS SAAT SE & Co. KGaA Maize plants with improved disease resistance
WO2025162985A1 (en) 2024-01-30 2025-08-07 Basf Plant Science Company Gmbh Increased plant disease resistance by expression of a glycine-rich protein
WO2025168705A1 (en) 2024-02-08 2025-08-14 Vib Vzw Means and methods for the production of saponins with endosomal escape-enhancing properties
WO2025171210A1 (en) 2024-02-09 2025-08-14 Arbor Biotechnologies, Inc. Compositions and methods for gene editing via homology-mediated end joining
WO2025174908A1 (en) 2024-02-12 2025-08-21 Life Edit Therapeutics, Inc. Novel rna-guided nucleases and proteins for polymerase editing
WO2025174765A1 (en) 2024-02-12 2025-08-21 Renagade Therapeutics Management Inc. Lipid nanoparticles comprising coding rna molecules for use in gene editing and as vaccines and therapeutic agents
WO2025172110A1 (en) 2024-02-16 2025-08-21 Nunhems Netherlands B.V. Modified plant architecture in cucumber
WO2025178902A1 (en) 2024-02-22 2025-08-28 Pairwise Plants Services, Inc. Methods and compositions for improving yield characteristics in plants
US12480141B2 (en) 2024-04-04 2025-11-25 Alia Therapeutics Srl Type V Cas proteins and applications thereof
WO2025224107A1 (en) 2024-04-22 2025-10-30 Basecamp Research Ltd Method and compositions for detecting off-target editing
WO2025224182A2 (en) 2024-04-23 2025-10-30 Basecamp Research Ltd Single construct platform for simultaneous delivery of gene editing machinery and nucleic acid cargo
WO2025235388A1 (en) 2024-05-06 2025-11-13 Regeneron Pharmaceuticals, Inc. Transgene genomic identification by nuclease-mediated long read sequencing
WO2025237806A1 (en) 2024-05-13 2025-11-20 Nunhems Netherlands B.V. Lettuce plant having delayed bolting and shade tolerance
WO2025240947A1 (en) 2024-05-17 2025-11-20 University Of Massachusetts Therapeutic treatment for fragile x-associated disorder
US12428636B1 (en) 2024-07-08 2025-09-30 Pairwise Plants Services, Inc. Methods and compositions for modification of protospacer adjacent motif specificity of CAS12A
CN119979720B (zh) * 2025-02-26 2025-09-23 中国农业科学院北京畜牧兽医研究所 猪精液品质相关的分子标记鉴定及其应用
CN120192429B (zh) * 2025-03-21 2025-12-12 华东理工大学 一种dhodh多肽降解剂及其在制备预防和/或治疗dhodh介导的疾病的药物中的应用

Family Cites Families (156)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1506509A (en) 1975-12-19 1978-04-05 Nissan Motor Apparatus for mounting instruments on the instrument panel of a motor vehicle
US4217344A (en) 1976-06-23 1980-08-12 L'oreal Compositions containing aqueous dispersions of lipid spheres
US4235871A (en) 1978-02-24 1980-11-25 Papahadjopoulos Demetrios P Method of encapsulating biologically active materials in lipid vesicles
US4186183A (en) 1978-03-29 1980-01-29 The United States Of America As Represented By The Secretary Of The Army Liposome carriers in chemotherapy of leishmaniasis
US4261975A (en) 1979-09-19 1981-04-14 Merck & Co., Inc. Viral liposome particle
US4485054A (en) 1982-10-04 1984-11-27 Lipoderm Pharmaceuticals Limited Method of encapsulating biologically active materials in multilamellar lipid vesicles (MLV)
US4501728A (en) 1983-01-06 1985-02-26 Technology Unlimited, Inc. Masking of liposomes from RES recognition
US4897355A (en) 1985-01-07 1990-01-30 Syntex (U.S.A.) Inc. N[ω,(ω-1)-dialkyloxy]- and N-[ω,(ω-1)-dialkenyloxy]-alk-1-yl-N,N,N-tetrasubstituted ammonium lipids and uses therefor
US4946787A (en) 1985-01-07 1990-08-07 Syntex (U.S.A.) Inc. N-(ω,(ω-1)-dialkyloxy)- and N-(ω,(ω-1)-dialkenyloxy)-alk-1-yl-N,N,N-tetrasubstituted ammonium lipids and uses therefor
US5049386A (en) 1985-01-07 1991-09-17 Syntex (U.S.A.) Inc. N-ω,(ω-1)-dialkyloxy)- and N-(ω,(ω-1)-dialkenyloxy)Alk-1-YL-N,N,N-tetrasubstituted ammonium lipids and uses therefor
US4797368A (en) 1985-03-15 1989-01-10 The United States Of America As Represented By The Department Of Health And Human Services Adeno-associated virus as eukaryotic expression vector
US4774085A (en) 1985-07-09 1988-09-27 501 Board of Regents, Univ. of Texas Pharmaceutical administration systems containing a mixture of immunomodulators
ATE141646T1 (de) 1986-04-09 1996-09-15 Genzyme Corp Genetisch transformierte tiere, die ein gewünschtes protein in milch absondern
US4837028A (en) 1986-12-24 1989-06-06 Liposome Technology, Inc. Liposomes with enhanced circulation time
US4873316A (en) 1987-06-23 1989-10-10 Biogen, Inc. Isolation of exogenous recombinant proteins from the milk of transgenic mammals
US5703055A (en) 1989-03-21 1997-12-30 Wisconsin Alumni Research Foundation Generation of antibodies through lipid mediated DNA delivery
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5264618A (en) 1990-04-19 1993-11-23 Vical, Inc. Cationic lipids for intracellular delivery of biologically active molecules
WO1991017424A1 (en) 1990-05-03 1991-11-14 Vical, Inc. Intracellular delivery of biologically active substances by means of self-assembling lipid complexes
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5266317A (en) 1990-10-04 1993-11-30 University Of Georgia Research Foundation, Inc. Insect-specific paralytic neurotoxin genes for use in biological insect control: methods and compositions
US5173414A (en) 1990-10-30 1992-12-22 Applied Immune Sciences, Inc. Production of recombinant adeno-associated virus vectors
GB9104617D0 (en) 1991-03-05 1991-04-17 Nickerson Int Seed Pest control
GB9114259D0 (en) 1991-07-02 1991-08-21 Ici Plc Plant derived enzyme and dna sequences
GB9115909D0 (en) 1991-07-23 1991-09-04 Nickerson Int Seed Recombinant dna
DE4124514A1 (de) 1991-07-24 1993-01-28 Bosch Gmbh Robert Beschleunigungssensor, insbesondere zum selbsttaetigen ausloesen von insassenschutzvorrichtungen in kraftfahrzeugen
DK39692D0 (da) 1992-03-25 1992-03-25 Danisco Biologisk materiale
US5587308A (en) 1992-06-02 1996-12-24 The United States Of America As Represented By The Department Of Health & Human Services Modified adeno-associated virus vector capable of expression from a novel promoter
HUT70467A (en) 1992-07-27 1995-10-30 Pioneer Hi Bred Int An improved method of agrobactenium-mediated transformation of cultvred soyhean cells
US5593972A (en) 1993-01-26 1997-01-14 The Wistar Institute Genetic immunization
US5814618A (en) 1993-06-14 1998-09-29 Basf Aktiengesellschaft Methods for regulating gene expression
US5789156A (en) 1993-06-14 1998-08-04 Basf Ag Tetracycline-regulated transcriptional inhibitors
US5543158A (en) 1993-07-23 1996-08-06 Massachusetts Institute Of Technology Biodegradable injectable nanoparticles
US6007845A (en) 1994-07-22 1999-12-28 Massachusetts Institute Of Technology Nanoparticles and microparticles of non-linear hydrophilic-hydrophobic multiblock copolymers
US5994627A (en) 1995-03-31 1999-11-30 Common Wealth Scientific And Industrial Research Organisation Genetic sequences conferring nematode resistance in plants and uses therefor
JPH10510433A (ja) 1995-06-06 1998-10-13 アイシス・ファーマシューティカルス・インコーポレーテッド 高いキラル純度のホスホロチオエート結合を有するオリゴヌクレオチド
US5985662A (en) 1995-07-13 1999-11-16 Isis Pharmaceuticals Inc. Antisense inhibition of hepatitis B virus replication
AR006928A1 (es) 1996-05-01 1999-09-29 Pioneer Hi Bred Int Una molecula de adn aislada que codifica una proteina fluorescente verde como marcador rastreable para la transformacion de plantas, un metodo para laproduccion de plantas transgenicas, un vector de expresion, una planta transgenica y celulas de dichas plantas.
US5855913A (en) 1997-01-16 1999-01-05 Massachusetts Instite Of Technology Particles incorporating surfactants for pulmonary drug delivery
US5985309A (en) 1996-05-24 1999-11-16 Massachusetts Institute Of Technology Preparation of particles for inhalation
US5846946A (en) 1996-06-14 1998-12-08 Pasteur Merieux Serums Et Vaccins Compositions and methods for administering Borrelia DNA
GB9907461D0 (en) 1999-03-31 1999-05-26 King S College London Neurite regeneration
US7022896B1 (en) 1997-04-04 2006-04-04 Board Of Regents Of University Of Nebraska Methods and materials for making and using transgenic dicamba-degrading organisms
GB9720465D0 (en) 1997-09-25 1997-11-26 Oxford Biomedica Ltd Dual-virus vectors
WO1999021977A1 (en) 1997-10-24 1999-05-06 Life Technologies, Inc. Recombinational cloning using nucleic acids having recombination sites
US6750059B1 (en) 1998-07-16 2004-06-15 Whatman, Inc. Archiving of vectors
US6534261B1 (en) 1999-01-12 2003-03-18 Sangamo Biosciences, Inc. Regulation of endogenous gene expression in cells using zinc finger proteins
US7868149B2 (en) 1999-07-20 2011-01-11 Monsanto Technology Llc Plant genome sequence and uses thereof
US6603061B1 (en) 1999-07-29 2003-08-05 Monsanto Company Agrobacterium-mediated plant transformation method
GB0024550D0 (es) 2000-10-06 2000-11-22 Oxford Biomedica Ltd
US7776321B2 (en) 2001-09-26 2010-08-17 Mayo Foundation For Medical Education And Research Mutable vaccines
GB0125216D0 (en) 2001-10-19 2001-12-12 Univ Strathclyde Dendrimers for use in targeted delivery
US20090100536A1 (en) 2001-12-04 2009-04-16 Monsanto Company Transgenic plants with enhanced agronomic traits
EP1458879A2 (en) 2001-12-21 2004-09-22 Oxford Biomedica (UK) Limited Method for producing a transgenic organism using a lentiviral expression vector such as eiav
EP1519714B1 (en) 2002-06-28 2010-10-20 Protiva Biotherapeutics Inc. Method and apparatus for producing liposomes
GB0220467D0 (en) 2002-09-03 2002-10-09 Oxford Biomedica Ltd Composition
AU2003284388A1 (en) 2002-11-01 2004-06-07 New England Biolabs, Inc. Organellar targeting of rna and its use in the interruption of environmental gene flow
ES2559828T3 (es) 2003-07-16 2016-02-16 Protiva Biotherapeutics Inc. ARN de interferencia encapsulado en lípidos
NZ581166A (en) 2003-09-15 2011-06-30 Protiva Biotherapeutics Inc Polyethyleneglycol-modified lipid compounds and uses thereof
GB0325379D0 (en) 2003-10-30 2003-12-03 Oxford Biomedica Ltd Vectors
US7919277B2 (en) * 2004-04-28 2011-04-05 Danisco A/S Detection and typing of bacterial strains
WO2005121348A1 (en) 2004-06-07 2005-12-22 Protiva Biotherapeutics, Inc. Lipid encapsulated interfering rna
US7745651B2 (en) 2004-06-07 2010-06-29 Protiva Biotherapeutics, Inc. Cationic lipids and methods of use
JP4772045B2 (ja) 2004-07-16 2011-09-14 アメリカ合衆国 Ｃｍｖ／ｒ核酸コンストラクトを含むａｉｄｓに対するワクチン
GB0422877D0 (en) 2004-10-14 2004-11-17 Univ Glasgow Bioactive polymers
WO2007048046A2 (en) 2005-10-20 2007-04-26 Protiva Biotherapeutics, Inc. Sirna silencing of filovirus gene expression
ES2377660T3 (es) 2005-10-28 2012-03-29 Mitsubishi Tanabe Pharma Corporation Nuevo péptido que penetra en las células
CN101346393B (zh) 2005-11-02 2015-07-22 普洛体维生物治疗公司 修饰的siRNA分子及其应用
GB0526211D0 (en) 2005-12-22 2006-02-01 Oxford Biomedica Ltd Viral vectors
US7915399B2 (en) 2006-06-09 2011-03-29 Protiva Biotherapeutics, Inc. Modified siRNA molecules and uses thereof
JP2008078613A (ja) 2006-08-24 2008-04-03 Rohm Co Ltd 窒化物半導体の製造方法及び窒化物半導体素子
US20100099858A1 (en) 2006-09-28 2010-04-22 Mirkin Chad A Maximizing Oligonucleotide Loading on Gold Nanoparticle
WO2008084405A2 (en) 2007-01-11 2008-07-17 Erasmus University Medical Center Circular chromosome conformation capture (4c)
US7838729B2 (en) 2007-02-26 2010-11-23 Monsanto Technology Llc Chloroplast transit peptides for efficient targeting of DMO and uses thereof
WO2008149176A1 (en) 2007-06-06 2008-12-11 Cellectis Meganuclease variants cleaving a dna target sequence from the mouse rosa26 locus and uses thereof
CN103083794B (zh) 2007-08-14 2016-03-02 弗雷德哈钦森癌症研究中心 用于递送治疗药物的针阵列组件和方法
CA2930393C (en) 2007-12-04 2022-11-29 Alnylam Pharmaceuticals, Inc. Carbohydrate conjugates as delivery agents for oligonucleotides
NZ587060A (en) 2007-12-31 2012-09-28 Nanocor Therapeutics Inc Rna interference for the treatment of heart failure
PL2279254T3 (pl) 2008-04-15 2017-11-30 Protiva Biotherapeutics Inc. Nowe preparaty lipidowe do dostarczania kwasów nukleinowych
FR2930264B1 (fr) * 2008-04-18 2013-02-22 Gervais Danone Sa Nouvelle souche de lactobacillus paracasei subsp. paracasei dotee de proprietes antimicrobiennes et immunomodulatrices.
US8575305B2 (en) 2008-06-04 2013-11-05 Medical Research Council Cell penetrating peptides
CN102137658A (zh) 2008-06-30 2011-07-27 斯兰斯德有限公司 局部递送药物的方法、组合物和系统
EP2309980A1 (en) 2008-07-08 2011-04-20 S.I.F.I. Societa' Industria Farmaceutica Italiana Ophthalmic compositions for treating pathologies of the posterior segment of the eye
US20100076057A1 (en) 2008-09-23 2010-03-25 Northwestern University TARGET DNA INTERFERENCE WITH crRNA
JP6087504B2 (ja) 2008-11-07 2017-03-01 マサチューセッツ インスティテュート オブ テクノロジー アミノアルコールリピドイドおよびその使用
GB2465749B (en) 2008-11-25 2013-05-08 Algentech Sas Plant cell transformation method
WO2010129602A2 (en) 2009-05-04 2010-11-11 Fred Hutchinson Cancer Research Center Cocal vesiculovirus envelope pseudotyped retroviral vectors
JP5766188B2 (ja) 2009-07-01 2015-08-19 プロチバ バイオセラピューティクス インコーポレイティッド 固形腫瘍に治療剤を送達するための脂質製剤
WO2011000108A1 (en) 2009-07-01 2011-01-06 Protiva Biotherapeutics, Inc. Compositions and methods for silencing apolipoprotein b
WO2011028929A2 (en) 2009-09-03 2011-03-10 The Regents Of The University Of California Nitrate-responsive promoter
US8889394B2 (en) 2009-09-07 2014-11-18 Empire Technology Development Llc Multiple domain proteins
SG10201407996PA (en) 2009-12-23 2015-01-29 Novartis Ag Lipids, lipid compositions, and methods of using them
SG183374A1 (en) 2010-02-24 2012-09-27 Arrowhead Res Corp Compositions for targeted delivery of sirna
SG185367A1 (en) 2010-04-26 2012-12-28 Sangamo Biosciences Inc Genome editing of a rosa locus using zinc-finger nucleases
US8372951B2 (en) 2010-05-14 2013-02-12 National Tsing Hua University Cell penetrating peptides for intracellular delivery
WO2011148194A1 (en) 2010-05-28 2011-12-01 Oxford Biomedica (Uk) Ltd Delivery of lentiviral vectors to the brain
EP2609135A4 (en) 2010-08-26 2015-05-20 Massachusetts Inst Technology POLY (BETA AMINO ALCOHOLS), THEIR PREPARATION AND USES THEREOF
EA201390586A1 (ru) * 2010-10-20 2014-11-28 ДюПон НЬЮТРИШН БАЙОСАЙЕНСИЗ АпС Последовательности crispr-cas lactococcus
US9405700B2 (en) 2010-11-04 2016-08-02 Sonics, Inc. Methods and apparatus for virtualization in an integrated circuit
PL2691443T3 (pl) 2011-03-28 2021-08-30 Massachusetts Institute Of Technology Sprzężone lipomery i ich zastosowania
EP2691101A2 (en) 2011-03-31 2014-02-05 Moderna Therapeutics, Inc. Delivery and formulation of engineered nucleic acids
US20120295960A1 (en) 2011-05-20 2012-11-22 Oxford Biomedica (Uk) Ltd. Treatment regimen for parkinson's disease
BR112014007078B1 (pt) 2011-09-26 2018-03-06 Sumitomo Chemical Company, Limited Composição e método de controle de doença de plantas
DE12858350T1 (de) 2011-12-16 2021-10-07 Modernatx, Inc. Modifizierte mrna zusammensetzungen
US20130185823A1 (en) 2012-01-16 2013-07-18 Academia Sinica Mesoporous silica nanoparticle-mediated delivery of dna into arabidopsis root
US9637739B2 (en) * 2012-03-20 2017-05-02 Vilnius University RNA-directed DNA cleavage by the Cas9-crRNA complex
JP2015515530A (ja) 2012-04-18 2015-05-28 アローヘッド リサーチ コーポレイション インビボ核酸送達のためのポリ（アクリラート）ポリマー
RS59199B1 (sr) 2012-05-25 2019-10-31 Univ California Metode i jedinjenja za rnk-upravljanu ciljanu dnk modifikaciju i za rnk- upravljanu modulaciju transkripta
EP3494997B1 (en) 2012-07-25 2019-09-18 The Broad Institute, Inc. Inducible dna binding proteins and genome perturbation tools and applications thereof
US8614194B1 (en) 2012-07-25 2013-12-24 Kaohsiung Medical University Anionic cell penetrating peptide and its use for intracellular delivery
UA119135C2 (uk) * 2012-09-07 2019-05-10 ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі Спосіб отримання трансгенної рослини
EP2898075B1 (en) 2012-12-12 2016-03-09 The Broad Institute, Inc. Engineering and optimization of improved systems, methods and enzyme compositions for sequence manipulation
WO2014093701A1 (en) 2012-12-12 2014-06-19 The Broad Institute, Inc. Functional genomics using crispr-cas systems, compositions, methods, knock out libraries and applications thereof
US8697359B1 (en) * 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products
IL239344B2 (en) 2012-12-12 2024-06-01 Broad Inst Inc Systems engineering, methods and optimal guiding components for sequence manipulation
EP4299741A3 (en) 2012-12-12 2024-02-28 The Broad Institute, Inc. Delivery, engineering and optimization of systems, methods and compositions for sequence manipulation and therapeutic applications
US20140310830A1 (en) * 2012-12-12 2014-10-16 Feng Zhang CRISPR-Cas Nickase Systems, Methods And Compositions For Sequence Manipulation in Eukaryotes
JP2016505256A (ja) 2012-12-12 2016-02-25 ザ・ブロード・インスティテュート・インコーポレイテッ 配列操作のためのＣＲＩＳＰＲ−Ｃａｓ成分系、方法および組成物
JP6700788B2 (ja) 2012-12-17 2020-05-27 プレジデント アンド フェローズ オブ ハーバード カレッジ Ｒｎａ誘導性ヒトゲノム改変
WO2014118272A1 (en) 2013-01-30 2014-08-07 Santaris Pharma A/S Antimir-122 oligonucleotide carbohydrate conjugates
NZ712727A (en) 2013-03-14 2017-05-26 Caribou Biosciences Inc Compositions and methods of nucleic acid-targeting nucleic acids
US11685935B2 (en) 2013-05-29 2023-06-27 Cellectis Compact scaffold of Cas9 in the type II CRISPR system
EP3003392B1 (en) * 2013-06-04 2019-10-23 President and Fellows of Harvard College Rna-guideded transcriptional regulation
US9267135B2 (en) 2013-06-04 2016-02-23 President And Fellows Of Harvard College RNA-guided transcriptional regulation
US10704060B2 (en) * 2013-06-05 2020-07-07 Duke University RNA-guided gene editing and gene regulation
AU2014281026B2 (en) 2013-06-17 2020-05-28 Massachusetts Institute Of Technology Delivery, engineering and optimization of tandem guide systems, methods and compositions for sequence manipulation
CN105492611A (zh) 2013-06-17 2016-04-13 布罗德研究所有限公司 用于序列操纵的优化的crispr-cas双切口酶系统、方法以及组合物
BR122021009076B1 (pt) * 2013-06-17 2024-02-15 The Broad Institute Inc. Vetor viral contendo molécula(s) de ácido nucleico heterólogo, composição, uso e métodos do mesmo
US10563225B2 (en) 2013-07-26 2020-02-18 President And Fellows Of Harvard College Genome engineering
US9228207B2 (en) 2013-09-06 2016-01-05 President And Fellows Of Harvard College Switchable gRNAs comprising aptamers
WO2015040402A1 (en) 2013-09-18 2015-03-26 Kymab Limited Methods. cells & organisms
CN111218447B (zh) 2013-11-07 2024-10-11 爱迪塔斯医药有限公司 使用统治型gRNA的CRISPR相关方法和组合物
US20160298096A1 (en) 2013-11-18 2016-10-13 Crispr Therapeutics Ag Crispr-cas system materials and methods
WO2015089486A2 (en) 2013-12-12 2015-06-18 The Broad Institute Inc. Systems, methods and compositions for sequence manipulation with optimized functional crispr-cas systems
WO2015089364A1 (en) 2013-12-12 2015-06-18 The Broad Institute Inc. Crystal structure of a crispr-cas system, and uses thereof
EP3653703A1 (en) 2013-12-12 2020-05-20 The Broad Institute, Inc. Compositions and methods of use of crispr-cas systems in nucleotide repeat disorders
EP3080259B1 (en) 2013-12-12 2023-02-01 The Broad Institute, Inc. Engineering of systems, methods and optimized guide compositions with new architectures for sequence manipulation
SG10201804977UA (en) 2013-12-12 2018-07-30 Broad Inst Inc Delivery, Use and Therapeutic Applications of the Crispr-Cas Systems and Compositions for Targeting Disorders and Diseases Using Particle Delivery Components
CA2933134A1 (en) 2013-12-13 2015-06-18 Cellectis Cas9 nuclease platform for microalgae genome engineering
US10557146B2 (en) 2014-01-21 2020-02-11 The Institute Of Genetics And Developmental Biology, Chinese Academy Of Sciences Modified plants
JP5937635B2 (ja) 2014-03-28 2016-06-22 ファナック株式会社 電磁接触器の溶着検出機能を有するモータ駆動装置
CN104017821B (zh) * 2014-05-16 2016-07-06 安徽省农业科学院水稻研究所 定向编辑颖壳颜色决定基因OsCHI创制褐壳水稻材料的方法
WO2016049258A2 (en) 2014-09-25 2016-03-31 The Broad Institute Inc. Functional screening with optimized functional crispr-cas systems
WO2016094872A1 (en) 2014-12-12 2016-06-16 The Broad Institute Inc. Dead guides for crispr transcription factors
SG11201704272YA (en) * 2014-12-31 2017-06-29 Synthetic Genomics Inc Compositions and methods for high efficiency in vivo genome editing
HUE063813T2 (hu) * 2015-01-28 2024-02-28 Caribou Biosciences Inc CRISPR hibrid DNS/RNS polinukleotidok és alkalmazási eljárások
GB201506509D0 (en) 2015-04-16 2015-06-03 Univ Wageningen Nuclease-mediated genome editing
US20160362667A1 (en) * 2015-06-10 2016-12-15 Caribou Biosciences, Inc. CRISPR-Cas Compositions and Methods
CA3012607A1 (en) * 2015-06-18 2016-12-22 The Broad Institute Inc. Crispr enzymes and systems
US9790490B2 (en) 2015-06-18 2017-10-17 The Broad Institute Inc. CRISPR enzymes and systems
CA3012631A1 (en) * 2015-06-18 2016-12-22 The Broad Institute Inc. Novel crispr enzymes and systems
CA2998287A1 (en) 2015-09-24 2017-04-20 Crispr Therapeutics Ag Novel family of rna-programmable endonucleases and their uses in genome editing and other applications
US12110490B2 (en) 2015-12-18 2024-10-08 The Broad Institute, Inc. CRISPR enzymes and systems
CN105602993A (zh) * 2016-01-19 2016-05-25 上海赛墨生物技术有限公司 线粒体靶向的基因编辑系统及方法
WO2017189308A1 (en) 2016-04-19 2017-11-02 The Broad Institute Inc. Novel crispr enzymes and systems
US20230037794A1 (en) 2019-12-23 2023-02-09 The Broad Institute, Inc. Programmable dna nuclease-associated ligase and methods of use thereof

Also Published As

Publication number Publication date
DK3604532T3 (da) 2024-07-22
IL305044B1 (en) 2025-01-01
RU2018101666A (ru) 2019-07-19
TWI758251B (zh) 2022-03-21
US10669540B2 (en) 2020-06-02
SG10201912330QA (en) 2020-02-27
CN109207477B (zh) 2021-07-16
KR102613296B1 (ko) 2023-12-13
TW201716572A (zh) 2017-05-16
ES2791398T3 (es) 2020-11-04
CN115572727A (zh) 2023-01-06
EP3502253A1 (en) 2019-06-26
JP2018518183A (ja) 2018-07-12
AU2024202007A1 (en) 2024-04-18
CN108513582A (zh) 2018-09-07
EP3009511A3 (en) 2016-06-29
ES2791195T3 (es) 2020-11-03
EP3310917A1 (en) 2018-04-25
WO2016205711A1 (en) 2016-12-22
EP4403638A2 (en) 2024-07-24
JP7731398B2 (ja) 2025-08-29
CN108513582B (zh) 2022-08-05
DK3310917T3 (da) 2020-05-04
US20180155716A1 (en) 2018-06-07
RU2018101666A3 (es) 2020-04-28
EP3009511B1 (en) 2017-05-31
EP4403638A3 (en) 2025-03-26
IL290678A (en) 2022-04-01
IL290678B1 (en) 2023-09-01
RU2020136363A (ru) 2020-12-24
CN115572718A (zh) 2023-01-06
EP3502253B1 (en) 2020-05-27
ES2646140T3 (es) 2017-12-12
US9790490B2 (en) 2017-10-17
IL256369A (en) 2018-02-28
EP4403638A9 (en) 2025-10-29
MX2020011939A (es) 2021-01-29
JP7280312B2 (ja) 2023-05-23
TW202426636A (zh) 2024-07-01
CA2989834A1 (en) 2016-12-22
JP6898865B2 (ja) 2021-07-07
EP3009511B2 (en) 2021-01-27
RU2737537C2 (ru) 2020-12-01
IL305044A (en) 2023-10-01
JP2021151247A (ja) 2021-09-30
ES2994064T3 (en) 2025-01-16
DK3009511T3 (da) 2017-09-11
FI3604532T3 (fi) 2024-08-05
CN109207477A (zh) 2019-01-15
DK3470519T3 (da) 2020-05-04
JP2023100906A (ja) 2023-07-19
FI3310917T4 (fi) 2025-12-10
IL256369B (en) 2022-03-01
MX2017016688A (es) 2018-12-11
EP3470519A1 (en) 2019-04-17
EP3604532A1 (en) 2020-02-05
TW202223088A (zh) 2022-06-16
KR20180034402A (ko) 2018-04-04
AU2021200010B2 (en) 2024-01-18
EP3502253B2 (en) 2025-11-19
EP3310917B1 (en) 2020-02-12
EP3009511A2 (en) 2016-04-20
KR20230170151A (ko) 2023-12-18
IL290678B2 (en) 2024-01-01
HK1253001A1 (en) 2019-06-06
DK3502253T3 (da) 2020-08-17
US20160208243A1 (en) 2016-07-21
JP2025179085A (ja) 2025-12-09
EP3470519B1 (en) 2020-02-12
EP3604532B1 (en) 2024-05-01
TWI837592B (zh) 2024-04-01
DK3310917T4 (da) 2025-12-15
IL305044B2 (en) 2025-05-01
AU2016278990B2 (en) 2020-10-22
AU2016278990A1 (en) 2018-01-18
CN114032250A (zh) 2022-02-11
EP3310917B2 (en) 2025-09-03
CN115572737A (zh) 2023-01-06
HK1220726A1 (en) 2017-05-12
AU2021200010A1 (en) 2021-02-11

Similar Documents

Publication Publication Date Title
MX377162B (es) Nuevas enzimas y sistemas crispr
AU2018251801A8 (en) Novel type VI CRISPR orthologs and systems
EP4361261A3 (en) Novel cas13b orthologues crispr enzymes and systems
WO2016205749A8 (en) Novel crispr enzymes and systems
WO2017127807A8 (en) Crystal structure of crispr cpf1
EP4253551A3 (en) Novel crispr dna and rna targeting enzymes and systems
WO2019006471A3 (en) NOVEL CRISPR RNA TARGETING ENZYMES, SYSTEMS AND USES THEREOF
BR112018076190A2 (pt) ortólogos e sistemas crispr tipo vi
EP4257696A3 (en) Novel crispr dna targeting enzymes and systems
WO2017184786A8 (en) Cpf1 complexes with reduced indel activity
ZA202202628B (en) Novel crispr dna targeting enzymes and systems
WO2015191693A3 (en) Method for gene editing
WO2016065349A8 (en) Short non-coding protein regulatory rnas (sprrnas) and methods of use
WO2018094356A3 (en) Compositions and methods for target nucleic acid modification
MX391311B (es) Acidos nucleicos modificados dirigidos a acidos nucleicos.
WO2015160895A3 (en) Modified transposases for improved insertion sequence bias and increased dna input tolerance
WO2015023975A8 (en) Compositions and methods for modulating rna
WO2016154127A3 (en) Compositions and methods for treating hypertriglyceridemia
WO2016049258A3 (en) Functional screening with optimized functional crispr-cas systems
EP4410805A3 (en) Raav-based compositions and methods for treating amyotrophic lateral sclerosis
WO2015196128A3 (en) Alternative nucleic acid molecules and uses thereof
WO2015196130A3 (en) Alternative nucleic acid molecules and uses thereof
EP4219704A3 (en) Aprl-clade protease variants and uses thereof
WO2020030984A3 (en) Compositions and methods for genome engineering with cas12a proteins
WO2016118726A3 (en) Methods and compositions for identification of highly specific nucleases

Legal Events

Date Code Title Description
FG Grant or registration