MX377162B - Nuevas enzimas y sistemas crispr - Google Patents

Nuevas enzimas y sistemas crispr

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Publication number
MX377162B
MX377162B MX2017016688A MX2017016688A MX377162B MX 377162 B MX377162 B MX 377162B MX 2017016688 A MX2017016688 A MX 2017016688A MX 2017016688 A MX2017016688 A MX 2017016688A MX 377162 B MX377162 B MX 377162B
Authority
MX
Mexico
Prior art keywords
methods
systems
targeting
crispr systems
new enzymes
Prior art date
Application number
MX2017016688A
Other languages
English (en)
Other versions
MX2017016688A (es
Inventor
Bernd Zetsche
Feng Zhang
Ian Slaymaker
Jonathan S Gootenberg
Omar O Abudayyeh
Original Assignee
Broad Inst Inc
Massachusetts Inst Technology
Harvard College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=55274964&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX377162(B) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Broad Inst Inc, Massachusetts Inst Technology, Harvard College filed Critical Broad Inst Inc
Publication of MX2017016688A publication Critical patent/MX2017016688A/es
Publication of MX377162B publication Critical patent/MX377162B/es

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    • C12N15/90—Stable introduction of foreign DNA into chromosome
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    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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    • C12N2310/00—Structure or type of the nucleic acid
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    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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Abstract

La presente invención se refiere a sistemas, métodos y composiciones para el direccionamiento de ácidos nucleicos. En particular, la invención provee sistemas de direccionamiento de adn no natural o manipulado que comprende una nueva proteína efectora crispr de direccionamiento de adn o arn y por lo menos un componente de ácido nucleico de direccionamiento tal como un arn guía. También se divulgan y reivindican métodos para elaborar y utilizar, y los usos, de dichos sistemas, métodos y composiciones, y productos de dichos métodos y usos.
MX2017016688A 2015-06-18 2016-06-17 Nuevas enzimas y sistemas crispr MX377162B (es)

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US201562181739P 2015-06-18 2015-06-18
US201562193507P 2015-07-16 2015-07-16
US201562201542P 2015-08-05 2015-08-05
US201562205733P 2015-08-16 2015-08-16
US201562232067P 2015-09-24 2015-09-24
US14/975,085 US9790490B2 (en) 2015-06-18 2015-12-18 CRISPR enzymes and systems
EP16150428.7A EP3009511B2 (en) 2015-06-18 2016-01-07 Novel crispr enzymes and systems
PCT/US2016/038181 WO2016205711A1 (en) 2015-06-18 2016-06-17 Novel crispr enzymes and systems

Publications (2)

Publication Number Publication Date
MX2017016688A MX2017016688A (es) 2018-12-11
MX377162B true MX377162B (es) 2025-03-07

Family

ID=55274964

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2017016688A MX377162B (es) 2015-06-18 2016-06-17 Nuevas enzimas y sistemas crispr
MX2020011939A MX2020011939A (es) 2015-06-18 2017-12-18 Nuevas enzimas y sistemas crispr.

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MX2020011939A MX2020011939A (es) 2015-06-18 2017-12-18 Nuevas enzimas y sistemas crispr.

Country Status (16)

Country Link
US (2) US9790490B2 (es)
EP (6) EP3009511B2 (es)
JP (4) JP6898865B2 (es)
KR (2) KR102931008B1 (es)
CN (6) CN114032250A (es)
AU (3) AU2016278990B2 (es)
CA (1) CA2989834A1 (es)
DK (5) DK3009511T3 (es)
ES (4) ES2646140T3 (es)
FI (3) FI3502253T4 (es)
IL (3) IL305044B2 (es)
MX (2) MX377162B (es)
RU (1) RU2737537C2 (es)
SG (1) SG10201912330QA (es)
TW (3) TWI906782B (es)
WO (1) WO2016205711A1 (es)

Families Citing this family (850)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2734621B1 (en) 2011-07-22 2019-09-04 President and Fellows of Harvard College Evaluation and improvement of nuclease cleavage specificity
US10704021B2 (en) 2012-03-15 2020-07-07 Flodesign Sonics, Inc. Acoustic perfusion devices
US9458450B2 (en) 2012-03-15 2016-10-04 Flodesign Sonics, Inc. Acoustophoretic separation technology using multi-dimensional standing waves
EP3597741A1 (en) 2012-04-27 2020-01-22 Duke University Genetic correction of mutated genes
SG10201806573TA (en) 2012-05-25 2018-09-27 Cellectis Methods for engineering allogeneic and immunosuppressive resistant t cell for immunotherapy
IL289396B2 (en) 2013-03-15 2023-12-01 The General Hospital Coporation Using truncated guide rnas (tru-grnas) to increase specificity for rna-guided genome editing
US9828582B2 (en) 2013-03-19 2017-11-28 Duke University Compositions and methods for the induction and tuning of gene expression
CA2908697C (en) 2013-04-16 2023-12-12 Regeneron Pharmaceuticals, Inc. Targeted modification of rat genome
EP3597755A1 (en) * 2013-06-17 2020-01-22 The Broad Institute, Inc. Delivery, use and therapeutic applications of the crispr-cas systems and compositions for targeting disorders and diseases using viral components
US9163284B2 (en) 2013-08-09 2015-10-20 President And Fellows Of Harvard College Methods for identifying a target site of a Cas9 nuclease
US9359599B2 (en) 2013-08-22 2016-06-07 President And Fellows Of Harvard College Engineered transcription activator-like effector (TALE) domains and uses thereof
US9526784B2 (en) 2013-09-06 2016-12-27 President And Fellows Of Harvard College Delivery system for functional nucleases
US9322037B2 (en) 2013-09-06 2016-04-26 President And Fellows Of Harvard College Cas9-FokI fusion proteins and uses thereof
US9340800B2 (en) 2013-09-06 2016-05-17 President And Fellows Of Harvard College Extended DNA-sensing GRNAS
JP2016536021A (ja) 2013-11-07 2016-11-24 エディタス・メディシン，インコーポレイテッド ＣＲＩＳＰＲ関連方法および支配ｇＲＮＡのある組成物
SG10201700961TA (en) 2013-12-11 2017-04-27 Regeneron Pharma Methods and compositions for the targeted modification of a genome
US20150165054A1 (en) 2013-12-12 2015-06-18 President And Fellows Of Harvard College Methods for correcting caspase-9 point mutations
US9725710B2 (en) 2014-01-08 2017-08-08 Flodesign Sonics, Inc. Acoustophoresis device with dual acoustophoretic chamber
DE212015000061U1 (de) 2014-02-11 2017-09-03 The Regents Of The University Of Colorado, A Body Corporate CRISPR-ermöglichtes Multiplex Genom Engineering
JP6779866B2 (ja) 2014-06-13 2020-11-04 チルドレンズ メディカル センター コーポレイション ミトコンドリアを単離するための製品および方法
EP3161128B1 (en) 2014-06-26 2018-09-26 Regeneron Pharmaceuticals, Inc. Methods and compositions for targeted genetic modifications and methods of use
CA2956224A1 (en) 2014-07-30 2016-02-11 President And Fellows Of Harvard College Cas9 proteins including ligand-dependent inteins
EP3204496A1 (en) 2014-10-10 2017-08-16 Editas Medicine, Inc. Compositions and methods for promoting homology directed repair
DK3207124T3 (da) 2014-10-15 2019-08-12 Regeneron Pharma Fremgangsmåder og sammensætninger til generering eller bevaring af pluripotente celler
US11680268B2 (en) 2014-11-07 2023-06-20 Editas Medicine, Inc. Methods for improving CRISPR/Cas-mediated genome-editing
EP3221457B1 (en) 2014-11-21 2019-03-20 Regeneron Pharmaceuticals, Inc. Methods and compositions for targeted genetic modification using paired guide rnas
CN113699116A (zh) 2014-12-10 2021-11-26 明尼苏达大学董事会 用于治疗疾病的遗传修饰的细胞、组织和器官
JP6840077B2 (ja) 2014-12-19 2021-03-10 リジェネロン・ファーマシューティカルズ・インコーポレイテッドＲｅｇｅｎｅｒｏｎ Ｐｈａｒｍａｃｅｕｔｉｃａｌｓ， Ｉｎｃ． 単一ステップの複数標的化を通じた標的化された遺伝子修飾のための方法及び組成物
JP6929791B2 (ja) 2015-02-09 2021-09-01 デューク ユニバーシティ エピゲノム編集のための組成物および方法
US20180080051A1 (en) * 2015-03-31 2018-03-22 Exeligen Scientific, Inc. Cas 9 retroviral integrase and cas 9 recombinase systems for targeted incorporation of a dna sequence into a genome of a cell or organism
GB201506509D0 (en) 2015-04-16 2015-06-03 Univ Wageningen Nuclease-mediated genome editing
US11377651B2 (en) 2016-10-19 2022-07-05 Flodesign Sonics, Inc. Cell therapy processes utilizing acoustophoresis
US11708572B2 (en) 2015-04-29 2023-07-25 Flodesign Sonics, Inc. Acoustic cell separation techniques and processes
CA2986310A1 (en) 2015-05-11 2016-11-17 Editas Medicine, Inc. Optimized crispr/cas9 systems and methods for gene editing in stem cells
EP3294879A4 (en) * 2015-05-14 2019-02-20 University of Southern California OPTIMIZED GENETIZATION WITH A RECOMBINANT ENDONUCLEASE SYSTEM
WO2016201047A1 (en) 2015-06-09 2016-12-15 Editas Medicine, Inc. Crispr/cas-related methods and compositions for improving transplantation
US10648020B2 (en) 2015-06-18 2020-05-12 The Broad Institute, Inc. CRISPR enzymes and systems
WO2016205749A1 (en) * 2015-06-18 2016-12-22 The Broad Institute Inc. Novel crispr enzymes and systems
US9790490B2 (en) * 2015-06-18 2017-10-17 The Broad Institute Inc. CRISPR enzymes and systems
EP4159856A1 (en) 2015-06-18 2023-04-05 The Broad Institute, Inc. Novel crispr enzymes and systems
US11414657B2 (en) 2015-06-29 2022-08-16 Ionis Pharmaceuticals, Inc. Modified CRISPR RNA and modified single CRISPR RNA and uses thereof
WO2017015015A1 (en) * 2015-07-17 2017-01-26 Emory University Crispr-associated protein from francisella and uses related thereto
US11474085B2 (en) 2015-07-28 2022-10-18 Flodesign Sonics, Inc. Expanded bed affinity selection
US11459540B2 (en) 2015-07-28 2022-10-04 Flodesign Sonics, Inc. Expanded bed affinity selection
IL295616A (en) 2015-07-31 2022-10-01 Us Health Adapted cells and treatment methods
AU2016308339A1 (en) 2015-08-18 2018-04-12 Baylor College Of Medicine Methods and compositions for altering function and structure of chromatin loops and/or domains
US11530424B1 (en) * 2015-09-18 2022-12-20 University Of South Florida Efficient CRISPR/HDR-mediated knock-in system and method of use
EP3786294A1 (en) 2015-09-24 2021-03-03 Editas Medicine, Inc. Use of exonucleases to improve crispr/cas-mediated genome editing
US20190048340A1 (en) * 2015-09-24 2019-02-14 Crispr Therapeutics Ag Novel family of rna-programmable endonucleases and their uses in genome editing and other applications
DK3359184T3 (da) 2015-10-05 2020-06-15 Prec Biosciences Inc Genetisk modificerede celler, der omfatter et modificeret gen fra konstant alfaregion i human t-cellereceptor
DK3359660T3 (en) 2015-10-05 2020-02-17 Prec Biosciences Inc Engineered meganucleases with recognition sequences found in the human t cell receptor alpha constant region gene
CN108513580A (zh) * 2015-10-08 2018-09-07 哈佛学院董事及会员团体 多重基因组编辑
EP4400597A3 (en) * 2015-10-09 2024-10-16 Monsanto Technology LLC Novel rna-guided nucleases and uses thereof
US11970710B2 (en) 2015-10-13 2024-04-30 Duke University Genome engineering with Type I CRISPR systems in eukaryotic cells
AU2016340078A1 (en) * 2015-10-16 2018-04-12 Temple University - Of The Commonwealth System Of Higher Education Methods and compositions utilizing Cpf1 for RNA-guided gene editing
EP4434589A3 (en) 2015-10-23 2025-05-14 President and Fellows of Harvard College Evolved cas9 proteins for gene editing
WO2017070598A1 (en) 2015-10-23 2017-04-27 Caribou Biosciences, Inc. Engineered crispr class 2 cross-type nucleic-acid targeting nucleic acids
WO2017075335A1 (en) 2015-10-28 2017-05-04 Voyager Therapeutics, Inc. Regulatable expression using adeno-associated virus (aav)
JP2018532415A (ja) * 2015-11-06 2018-11-08 ザ ジャクソン ラボラトリー 大きなゲノムｄｎａノックインおよびその使用
US11905521B2 (en) * 2015-11-17 2024-02-20 The Chinese University Of Hong Kong Methods and systems for targeted gene manipulation
WO2017095946A1 (en) 2015-11-30 2017-06-08 Flagship Pioneering, Inc. Methods and compositions relating to chondrisomes
KR102787119B1 (ko) 2015-11-30 2025-03-27 듀크 유니버시티 유전자 편집에 의한 인간 디스트로핀 유전자의 교정을 위한 치료용 표적 및 사용 방법
US9988624B2 (en) 2015-12-07 2018-06-05 Zymergen Inc. Microbial strain improvement by a HTP genomic engineering platform
US11208649B2 (en) 2015-12-07 2021-12-28 Zymergen Inc. HTP genomic engineering platform
KR20180084756A (ko) 2015-12-07 2018-07-25 지머젠 인코포레이티드 코리네박테리움 글루타미컴으로부터의 프로모터
WO2017099494A1 (ko) * 2015-12-08 2017-06-15 기초과학연구원 Cpf1을 포함하는 유전체 교정용 조성물 및 그 용도
US12110490B2 (en) 2015-12-18 2024-10-08 The Broad Institute, Inc. CRISPR enzymes and systems
CN115216459B (zh) * 2015-12-29 2024-06-28 孟山都技术公司 新型crispr相关转座酶及其用途
WO2017123609A1 (en) * 2016-01-12 2017-07-20 The Regents Of The University Of California Compositions and methods for enhanced genome editing
FI4272834T3 (fi) 2016-01-15 2025-12-05 Childrens Medical Ct Corp Mitokondrioiden ja yhdistettyjen mitokondriaalisten aineiden terapeuttinen käyttö
JP6914274B2 (ja) * 2016-01-22 2021-08-04 ザ・ブロード・インスティテュート・インコーポレイテッド Ｃｒｉｓｐｒｃｐｆ１の結晶構造
US9896696B2 (en) * 2016-02-15 2018-02-20 Benson Hill Biosystems, Inc. Compositions and methods for modifying genomes
US11447768B2 (en) * 2016-03-01 2022-09-20 University Of Florida Research Foundation, Incorporated Molecular cell diary system
KR102587132B1 (ko) * 2016-03-04 2023-10-11 에디타스 메디신, 인코포레이티드 암 면역요법을 위한 crispr-cpf1-관련 방법, 조성물 및 구성성분
US11597924B2 (en) 2016-03-25 2023-03-07 Editas Medicine, Inc. Genome editing systems comprising repair-modulating enzyme molecules and methods of their use
WO2017180694A1 (en) 2016-04-13 2017-10-19 Editas Medicine, Inc. Cas9 fusion molecules gene editing systems, and methods of use thereof
US20190127713A1 (en) 2016-04-13 2019-05-02 Duke University Crispr/cas9-based repressors for silencing gene targets in vivo and methods of use
EP3445853A1 (en) 2016-04-19 2019-02-27 The Broad Institute, Inc. Cpf1 complexes with reduced indel activity
WO2017189308A1 (en) 2016-04-19 2017-11-02 The Broad Institute Inc. Novel crispr enzymes and systems
KR20260004568A (ko) 2016-04-19 2026-01-08 더 브로드 인스티튜트, 인코퍼레이티드 신규한 crispr 효소 및 시스템
US20190136211A1 (en) * 2016-04-28 2019-05-09 Industry-Academic Cooperation Foundation Yonsei University Method For In Vivo High-Throughput Evaluating Of RNA-Guided Nuclease Activity
US11085035B2 (en) 2016-05-03 2021-08-10 Flodesign Sonics, Inc. Therapeutic cell washing, concentration, and separation utilizing acoustophoresis
US11214789B2 (en) 2016-05-03 2022-01-04 Flodesign Sonics, Inc. Concentration and washing of particles with acoustics
WO2017195905A1 (ja) 2016-05-13 2017-11-16 株式会社カネカ 形質転換植物の作製方法
US11499158B2 (en) 2016-05-13 2022-11-15 Kaneka Corporation Method for modifying plant
CN109153988B (zh) * 2016-05-13 2023-04-18 株式会社钟化 植物的基因组编辑方法
CN109475109B (zh) 2016-05-20 2021-10-29 瑞泽恩制药公司 用于使用多个引导rna来破坏免疫耐受性的方法
CA3018294A1 (en) * 2016-06-01 2017-12-07 Excision Biotherapeutics, Inc. Compositions and methods of treatment for lytic and lysogenic viruses
JP2019517261A (ja) 2016-06-01 2019-06-24 カーヴェーエス ザート ソシエタス・ヨーロピアＫｗｓ Ｓａａｔ Ｓｅ ゲノム操作のためのハイブリッド核酸配列
KR102811233B1 (ko) 2016-06-02 2025-05-29 시그마-알드리치 컴퍼니., 엘엘씨 프로그램가능한 dna 결합 단백질을 사용한, 표적화된 게놈 변형의 개선
KR20190016970A (ko) 2016-06-14 2019-02-19 파이어니어 하이 부렛드 인터내쇼날 인코포레이팃드 식물 게놈 변형을 위한 cpf1 엔도뉴클레아제의 용도
US10337051B2 (en) 2016-06-16 2019-07-02 The Regents Of The University Of California Methods and compositions for detecting a target RNA
KR20190019168A (ko) * 2016-06-17 2019-02-26 더 브로드 인스티튜트, 인코퍼레이티드 제vi형 crispr 오솔로그 및 시스템
US11293021B1 (en) 2016-06-23 2022-04-05 Inscripta, Inc. Automated cell processing methods, modules, instruments, and systems
CN109688820B (zh) 2016-06-24 2023-01-10 科罗拉多州立大学董事会（法人团体） 用于生成条形码化组合文库的方法
KR102345899B1 (ko) 2016-06-30 2021-12-31 지머젠 인코포레이티드 박테리아 헤모글로빈 라이브러리를 생성하는 방법 및 이의 용도
JP2019519241A (ja) 2016-06-30 2019-07-11 ザイマージェン インコーポレイテッド グルコース透過酵素ライブラリーを生成するための方法およびその使用
CN106191057B (zh) * 2016-07-06 2018-12-25 中山大学 一种用于敲除人CYP2E1基因的sgRNA序列、CYP2E1基因缺失细胞株的构建方法及其应用
WO2018013720A1 (en) * 2016-07-12 2018-01-18 Washington University Incorporation of internal polya-encoded poly-lysine sequence tags and their variations for the tunable control of protein synthesis in bacterial and eukaryotic cells
CA3030587A1 (en) 2016-07-15 2018-01-18 Salk Institute For Biological Studies Methods and compositions for genome editing in non-dividing cells
EP4275747A3 (en) * 2016-07-19 2024-01-24 Duke University Therapeutic applications of cpf1-based genome editing
KR20190031306A (ko) 2016-07-21 2019-03-25 맥스시티 인코포레이티드 게놈 dna를 변경하기 위한 방법 및 조성물
CN109790527A (zh) * 2016-07-26 2019-05-21 通用医疗公司 普氏菌属和弗朗西斯氏菌属的CRISPR1(Cpf1)的变体
JP7231935B2 (ja) 2016-08-03 2023-03-08 プレジデント アンド フェローズ オブ ハーバード カレッジ アデノシン核酸塩基編集因子およびそれらの使用
US11078481B1 (en) 2016-08-03 2021-08-03 KSQ Therapeutics, Inc. Methods for screening for cancer targets
US11661590B2 (en) 2016-08-09 2023-05-30 President And Fellows Of Harvard College Programmable CAS9-recombinase fusion proteins and uses thereof
JP6799066B2 (ja) * 2016-08-10 2020-12-09 株式会社ＧｅｎＡｈｅａｄ Ｂｉｏ 真核細胞のゲノムの標的部位を改変する方法及び標的部位における検出対象核酸配列の存在又は非存在を検出する方法
WO2018035388A1 (en) 2016-08-17 2018-02-22 The Broad Institute, Inc. Novel crispr enzymes and systems
CN110312799A (zh) * 2016-08-17 2019-10-08 博德研究所 新型crispr酶和系统
WO2018039438A1 (en) 2016-08-24 2018-03-01 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
US11078483B1 (en) 2016-09-02 2021-08-03 KSQ Therapeutics, Inc. Methods for measuring and improving CRISPR reagent function
IL247752A0 (en) * 2016-09-11 2016-11-30 Yeda Res & Dev Compositions and methods for modulating gene expression for site-directed mutagenesis
US10650621B1 (en) 2016-09-13 2020-05-12 Iocurrents, Inc. Interfacing with a vehicular controller area network
US20190225974A1 (en) 2016-09-23 2019-07-25 BASF Agricultural Solutions Seed US LLC Targeted genome optimization in plants
WO2018064352A1 (en) * 2016-09-30 2018-04-05 The Regents Of The University Of California Rna-guided nucleic acid modifying enzymes and methods of use thereof
JP2019532644A (ja) * 2016-09-30 2019-11-14 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア Ｒｎａ誘導型核酸修飾酵素及びその使用方法
MA46354A (fr) 2016-10-03 2019-08-07 Juno Therapeutics Inc Molécules se liant spécifiquement au vph
ES2811500T3 (es) 2016-10-04 2021-03-12 Prec Biosciences Inc Dominios coestimuladores para su uso en células genéticamente modificadas
CN110462034A (zh) 2016-10-07 2019-11-15 综合Dna技术公司 化脓链球菌cas9突变基因和由其编码的多肽
US11242542B2 (en) 2016-10-07 2022-02-08 Integrated Dna Technologies, Inc. S. pyogenes Cas9 mutant genes and polypeptides encoded by same
EP3526326A4 (en) * 2016-10-12 2020-07-29 The Regents of The University of Colorado, A Body Corporate NEW MODIFIED AND CHEMERICAL NUCLEASES
CN110290813A (zh) * 2016-10-14 2019-09-27 通用医疗公司 表观遗传学调控的位点特异性核酸酶
AU2017342543B2 (en) 2016-10-14 2024-06-27 President And Fellows Of Harvard College AAV delivery of nucleobase editors
EP4338799A3 (en) 2016-10-18 2024-06-05 Regents of the University of Minnesota Tumor infiltrating lymphocytes and methods of therapy
CA3042259A1 (en) 2016-11-04 2018-05-11 Flagship Pioneering Innovations V. Inc. Novel plant cells, plants, and seeds
CN106591335A (zh) * 2016-11-08 2017-04-26 安徽省农业科学院水稻研究所 一种密码子植物化改造的LbCpf1基因及其应用
JP2019535287A (ja) 2016-11-22 2019-12-12 インテグレイテツド・デイー・エヌ・エイ・テクノロジーズ・インコーポレイテツド Ｃｒｉｓｐｒ／ｃｐｆ１システム及び方法
US20200046854A1 (en) * 2016-11-28 2020-02-13 The Board Of Regents Of The University Of Texas System Prevention of muscular dystrophy by crispr/cpf1-mediated gene editing
CN108130342B (zh) * 2016-12-01 2022-01-11 中国科学院分子植物科学卓越创新中心 基于Cpf1的植物基因组定点编辑方法
WO2018107103A1 (en) * 2016-12-09 2018-06-14 The Broad Institute, Inc. Crispr-systems for modifying a trait of interest in a plant
WO2018111947A1 (en) 2016-12-12 2018-06-21 Integrated Dna Technologies, Inc. Genome editing enhancement
CA3045134A1 (en) 2016-12-14 2018-06-21 Ligandal, Inc. Compositions and methods for nucleic acid and/or protein payload delivery
WO2018119010A1 (en) 2016-12-19 2018-06-28 Editas Medicine, Inc. Assessing nuclease cleavage
CA3047542A1 (en) * 2016-12-19 2018-06-28 The Uab Research Foundation Mass spectrometric standards for hemoglobin beta and hemoglobin beta sickle and uses thereof
EP3557981A1 (en) * 2016-12-22 2019-10-30 Keygene N.V. Method for targeted alteration of duplex dna
WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
WO2018119354A1 (en) * 2016-12-23 2018-06-28 President And Fellows Of Harvard College Gene editing of pcsk9
CN110300802A (zh) * 2016-12-23 2019-10-01 基础科学研究院 用于动物胚胎碱基编辑的组合物和碱基编辑方法
US20190382751A1 (en) * 2016-12-28 2019-12-19 Ionis Pharmaceuticals, Inc. Modified crispr rna and uses thereof
US11859219B1 (en) 2016-12-30 2024-01-02 Flagship Pioneering Innovations V, Inc. Methods of altering a target nucleotide sequence with an RNA-guided nuclease and a single guide RNA
WO2018129368A2 (en) 2017-01-06 2018-07-12 Editas Medicine, Inc. Methods of assessing nuclease cleavage
FI3565891T3 (fi) 2017-01-09 2023-07-04 Whitehead Inst Biomedical Res Menetelmiä geeniekspression muuttamiseksi häiritsemällä säätelysilmukoita muodostavia transkriptiotekijämultimeerejä
GB201700380D0 (en) * 2017-01-10 2017-02-22 Plant Bioscience Ltd Methods of increasing seed yield
US20180201921A1 (en) * 2017-01-18 2018-07-19 Excision Biotherapeutics, Inc. CRISPRs
EP3573448A4 (en) 2017-01-28 2020-09-02 Inari Agriculture, Inc. NEW PLANT CELLS, PLANTS AND SEEDS
DK3574101T5 (da) 2017-01-30 2024-09-02 Kws Saat Se & Co Kgaa Reparationsskabelonbinding til endonukleaser til genommodifikation
CN110312797A (zh) * 2017-02-10 2019-10-08 齐默尔根公司 组装和编辑用于多个宿主的多个dna构建体的模块化通用质粒设计策略
CA3053092A1 (en) * 2017-02-10 2018-08-16 National Agriculture And Food Research Organization Method for producing hsl protein having improved catalytic activity for 2-oxoglutaric acid-dependently oxidizing 4-hppd inhibitor
KR102772726B1 (ko) 2017-02-15 2025-02-25 키진 엔.브이. 식물 세포에서 표적 유전적 변형 방법
CN106868031A (zh) * 2017-02-24 2017-06-20 北京大学 一种基于分级组装的多个sgRNA串联并行表达的克隆方法及应用
CN106947779B (zh) * 2017-02-28 2021-02-26 中国农业大学 利用乳腺生物反应器提高乳汁中褪黑素含量的方法
US10687520B2 (en) 2017-03-07 2020-06-23 The Board Of Regents Of The University Of Texas System Generation and correction of a humanized mouse model with a deletion of dystrophin exon 44
CN110662556A (zh) * 2017-03-09 2020-01-07 哈佛大学的校长及成员们 癌症疫苗
US11898179B2 (en) 2017-03-09 2024-02-13 President And Fellows Of Harvard College Suppression of pain by gene editing
JP2020510439A (ja) 2017-03-10 2020-04-09 プレジデント アンド フェローズ オブ ハーバード カレッジ シトシンからグアニンへの塩基編集因子
CN106978428A (zh) * 2017-03-15 2017-07-25 上海吐露港生物科技有限公司 一种Cas蛋白特异结合靶标DNA、调控靶标基因转录的方法及试剂盒
CN120330163A (zh) * 2017-03-15 2025-07-18 博德研究所 新型cas13b直向同源物crispr酶和系统
WO2018176009A1 (en) * 2017-03-23 2018-09-27 President And Fellows Of Harvard College Nucleobase editors comprising nucleic acid programmable dna binding proteins
WO2018175712A1 (en) * 2017-03-23 2018-09-27 The Government Of The United States Of America As Represented By The Secretary Of The Army Recombinant adeno-associated virus-paraoxonase 1-ifii particles and the methods of making and using thereof
US12161694B2 (en) 2017-03-24 2024-12-10 The Broad Institute, Inc. Methods and compositions for regulating innate lymphoid cell inflammatory responses
US11963966B2 (en) 2017-03-31 2024-04-23 Dana-Farber Cancer Institute, Inc. Compositions and methods for treating ovarian tumors
CN108690845B (zh) * 2017-04-10 2021-04-27 中国科学院动物研究所 基因组编辑系统和方法
US11352637B2 (en) 2017-04-14 2022-06-07 Monsanto Technology Llc Methods and compositions for herbicide tolerance in plants
WO2018191715A2 (en) * 2017-04-14 2018-10-18 Synthetic Genomics, Inc. Polypeptides with type v crispr activity and uses thereof
US12058986B2 (en) 2017-04-20 2024-08-13 Egenesis, Inc. Method for generating a genetically modified pig with inactivated porcine endogenous retrovirus (PERV) elements
BR112019021719A2 (pt) * 2017-04-21 2020-06-16 The General Hospital Corporation Variantes de cpf1 (cas12a) com especificidade para pam alterada
AU2018254616B2 (en) * 2017-04-21 2022-07-28 The General Hospital Corporation Inducible, tunable, and multiplex human gene regulation using crispr-Cpf1
EP3615672A1 (en) 2017-04-28 2020-03-04 Editas Medicine, Inc. Methods and systems for analyzing guide rna molecules
CN110785489A (zh) * 2017-05-04 2020-02-11 宾夕法尼亚大学董事会 使用CRISPR/Cpf1在T细胞中进行基因编辑的组合物和方法
WO2018208837A1 (en) 2017-05-08 2018-11-15 Precision Biosciences, Inc. Nucleic acid molecules encoding an engineered antigen receptor and an inhibitory nucleic acid molecule and methods of use thereof
US12226479B2 (en) 2017-05-11 2025-02-18 The General Hospital Corporation Methods and compositions of use of CD8+ tumor infiltrating lymphocyte subtypes and gene signatures thereof
US11560566B2 (en) 2017-05-12 2023-01-24 President And Fellows Of Harvard College Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation
JP7364472B2 (ja) * 2017-05-18 2023-10-18 ザ・ブロード・インスティテュート・インコーポレイテッド 標的化された核酸編集のための系、方法、及び組成物
WO2018213726A1 (en) 2017-05-18 2018-11-22 The Broad Institute, Inc. Systems, methods, and compositions for targeted nucleic acid editing
US11046969B2 (en) 2017-05-24 2021-06-29 Epiplanta Biotech Ltd. Transgenic plant and the method for producing the same
KR102742817B1 (ko) * 2017-05-25 2024-12-17 리제너론 파마슈티칼스 인코포레이티드 Cblb 엔도뉴클레아제 변이체, 조성물 및 사용 방법
US11603544B2 (en) 2017-06-05 2023-03-14 Fred Hutchinson Cancer Center Genomic safe harbors for genetic therapies in human stem cells and engineered nanoparticles to provide targeted genetic therapies
EP3878961A1 (en) 2017-06-06 2021-09-15 Zymergen, Inc. A htp genomic engineering platform for improving escherichia coli
WO2018226900A2 (en) 2017-06-06 2018-12-13 Zymergen Inc. A htp genomic engineering platform for improving fungal strains
EP3635104A1 (en) 2017-06-09 2020-04-15 Editas Medicine, Inc. Engineered cas9 nucleases
AU2018285860A1 (en) 2017-06-13 2020-01-02 Flagship Pioneering Innovations V, Inc. Compositions comprising curons and uses thereof
WO2018232195A1 (en) 2017-06-14 2018-12-20 The Broad Institute, Inc. Compositions and methods targeting complement component 3 for inhibiting tumor growth
US9982279B1 (en) 2017-06-23 2018-05-29 Inscripta, Inc. Nucleic acid-guided nucleases
US10011849B1 (en) 2017-06-23 2018-07-03 Inscripta, Inc. Nucleic acid-guided nucleases
KR102321388B1 (ko) 2017-06-23 2021-11-03 인스크립타 인코포레이티드 핵산 가이드 뉴클레아제
AU2018290843B2 (en) 2017-06-26 2025-04-24 Massachusetts Institute Of Technology CRISPR/Cas-adenine deaminase based compositions, systems, and methods for targeted nucleic acid editing
CN107312790A (zh) * 2017-06-26 2017-11-03 中国科学技术大学 一种可编程的多位点特异性的转录抑制系统及其应用
AU2018291041B2 (en) 2017-06-30 2021-08-05 Inscripta, Inc. Automated cell processing methods, modules, instruments, and systems
US10392616B2 (en) 2017-06-30 2019-08-27 Arbor Biotechnologies, Inc. CRISPR RNA targeting enzymes and systems and uses thereof
CA3068465A1 (en) 2017-06-30 2019-01-03 Precision Biosciences, Inc. Genetically-modified t cells comprising a modified intron in the t cell receptor alpha gene
EP3645021A4 (en) 2017-06-30 2021-04-21 Intima Bioscience, Inc. ADENO-ASSOCIATED VIRAL VECTORS FOR GENE THERAPY
KR102007457B1 (ko) * 2017-06-30 2019-08-05 동국대학교 산학협력단 Cas9 단백질, 가이드 RNA 및 양친매성 펩타이드를 포함하는 나노복합체 및 이의 용도
WO2019010422A1 (en) * 2017-07-07 2019-01-10 The Broad Institute, Inc. ANTIVIRAL THERAPY BASED ON THE CRISPR SYSTEM
WO2019014581A1 (en) 2017-07-14 2019-01-17 The Broad Institute, Inc. METHODS AND COMPOSITIONS FOR MODULATING THE ACTIVITY OF A CYTOTOXIC LYMPHOCYTE
WO2019014564A1 (en) 2017-07-14 2019-01-17 Editas Medicine, Inc. SYSTEMS AND METHODS OF TARGETED INTEGRATION AND GENOME EDITING AND DETECTION THEREOF WITH INTEGRATED PRIMING SITES
CN107488710B (zh) * 2017-07-14 2020-09-22 上海吐露港生物科技有限公司 一种Cas蛋白的用途及靶标核酸分子的检测方法和试剂盒
EP3654993A4 (en) 2017-07-17 2021-08-25 The Broad Institute, Inc. CELL ATLAS OF HEALTHY HUMAN COLUMN AND HUMAN COLUMN WITH COLITIS ULCEROSA
EP3655530A4 (en) * 2017-07-17 2021-07-28 The Broad Institute, Inc. NEW TYPE VI CRISPR ORTHOLOGIST AND SYSTEMS
WO2019017988A1 (en) * 2017-07-21 2019-01-24 Arizona Board Of Regents On Behalf Of Arizona State University CRISPR FLUORESCENT GUIDED RNA (ARNFG) FOR UNDERSTANDING RNAs EXPRESSED FROM POL II PROMOTERS
EP3657980A4 (en) 2017-07-28 2021-04-28 Locus IP Company, LLC YEAST MASKS TO IMPROVE SKIN, HAIR AND SCALP HEALTH
US11732274B2 (en) 2017-07-28 2023-08-22 President And Fellows Of Harvard College Methods and compositions for evolving base editors using phage-assisted continuous evolution (PACE)
AU2018309714A1 (en) 2017-07-31 2020-01-30 Regeneron Pharmaceuticals, Inc. Assessment of CRISPR/Cas-induced recombination with an exogenous donor nucleic acid in vivo
EP3585160A2 (en) 2017-07-31 2020-01-01 Regeneron Pharmaceuticals, Inc. Crispr reporter non-human animals and uses thereof
SG11201912235PA (en) 2017-07-31 2020-01-30 Regeneron Pharma Cas-transgenic mouse embryonic stem cells and mice and uses thereof
IL271283B2 (en) 2017-08-04 2024-04-01 Syngenta Participations Ag Methods and compositions for targeted genomic insertion
US10316324B2 (en) 2017-08-09 2019-06-11 Benson Hill Biosystems, Inc. Compositions and methods for modifying genomes
US10476825B2 (en) 2017-08-22 2019-11-12 Salk Institue for Biological Studies RNA targeting methods and compositions
US11970720B2 (en) 2017-08-22 2024-04-30 Salk Institute For Biological Studies RNA targeting methods and compositions
EP3672612A4 (en) 2017-08-23 2021-09-29 The General Hospital Corporation GENETICALLY MODIFIED CRISPR-CAS9 NUCLEASES WITH MODIFIED PAM SPECIFICITY
US10738327B2 (en) 2017-08-28 2020-08-11 Inscripta, Inc. Electroporation cuvettes for automation
WO2019139645A2 (en) 2017-08-30 2019-07-18 President And Fellows Of Harvard College High efficiency base editors comprising gam
WO2019046540A1 (en) * 2017-08-31 2019-03-07 New York Genome Center, Inc. METHODS AND COMPOSITIONS COMPRISING CRISPR-CPF1 AND APPROVED GUIDING CRISPR RNAES FOR PROGRAMMABLE GENOMIC DELETIONS
KR20200066616A (ko) * 2017-09-21 2020-06-10 더 브로드 인스티튜트, 인코퍼레이티드 표적화된 핵산 편집을 위한 시스템, 방법 및 조성물
ES2962277T3 (es) 2017-09-29 2024-03-18 Regeneron Pharma Roedores que comprenden un locus Ttr humanizado y métodos de uso
US11845951B2 (en) 2017-09-29 2023-12-19 Toolgen Incorporated Gene manipulation for treatment of retinal dysfunction disorder
US10435713B2 (en) 2017-09-30 2019-10-08 Inscripta, Inc. Flow through electroporation instrumentation
US20200239544A1 (en) 2017-10-03 2020-07-30 Precision Biosciences, Inc. Modified epidermal growth factor receptor peptides for use in genetically-modified cells
CN111954679A (zh) 2017-10-03 2020-11-17 朱诺治疗学股份有限公司 Hpv特异性结合分子
CA3076518A1 (en) * 2017-10-04 2019-04-11 The Broad Institute, Inc. Crispr effector system based diagnostics
KR20200121782A (ko) 2017-10-16 2020-10-26 더 브로드 인스티튜트, 인코퍼레이티드 아데노신 염기 편집제의 용도
CA3079681A1 (en) 2017-10-20 2019-04-25 Fred Hutchinson Cancer Research Center Systems and methods to produce b cells genetically modified to express selected antibodies
CN117487776A (zh) 2017-11-01 2024-02-02 加利福尼亚大学董事会 Casz组合物和使用方法
WO2019089913A1 (en) 2017-11-01 2019-05-09 Precision Biosciences, Inc. Engineered nucleases that target human and canine factor viii genes as a treatment for hemophilia a
US11970719B2 (en) 2017-11-01 2024-04-30 The Regents Of The University Of California Class 2 CRISPR/Cas compositions and methods of use
WO2019089804A1 (en) 2017-11-01 2019-05-09 The Regents Of The University Of California Casy compositions and methods of use
CA3081346A1 (en) 2017-11-02 2019-05-09 Arbor Biotechnologies, Inc. Novel crispr-associated transposon systems and components
GB2568255A (en) * 2017-11-08 2019-05-15 Evox Therapeutics Ltd Exosomes comprising RNA therapeutics
WO2019094984A1 (en) 2017-11-13 2019-05-16 The Broad Institute, Inc. Methods for determining spatial and temporal gene expression dynamics during adult neurogenesis in single cells
US12171783B2 (en) 2017-11-13 2024-12-24 The Broad Institute, Inc. Methods and compositions for targeting developmental and oncogenic programs in H3K27M gliomas
JP7423520B2 (ja) 2017-11-16 2024-01-29 アストラゼネカ・アクチエボラーグ Ｃａｓ９ベースノックイン方針の効力を改善するための組成物及び方法
CN111601883B (zh) 2017-11-17 2024-06-21 艾欧凡斯生物治疗公司 由细针抽吸物和小活检物扩增til
JP2021503885A (ja) 2017-11-22 2021-02-15 アイオバンス バイオセラピューティクス，インコーポレイテッド 末梢血からの末梢血リンパ球（ｐｂｌ）の拡大培養
US10253365B1 (en) * 2017-11-22 2019-04-09 The Regents Of The University Of California Type V CRISPR/Cas effector proteins for cleaving ssDNAs and detecting target DNAs
WO2019108670A1 (en) * 2017-11-28 2019-06-06 Emendobio Inc. Differential knockout of an allele of a heterozygous fibrinogen alpha chain (fga) gene
US11332736B2 (en) 2017-12-07 2022-05-17 The Broad Institute, Inc. Methods and compositions for multiplexing single cell and single nuclei sequencing
CA3082956A1 (en) 2017-12-08 2019-06-13 Synthetic Genomics, Inc. Improving algal lipid productivity via genetic modification of a tpr domain containing protein
US11203752B2 (en) * 2017-12-11 2021-12-21 Pioneer Hi-Bred International, Inc. Compositions and methods of modifying a plant genome to produce a MS9, MS22, MS26, or MS45 male-sterile plant
JP2021507561A (ja) 2017-12-14 2021-02-22 フロデザイン ソニックス， インク．Ｆｌｏｄｅｓｉｇｎ Ｓｏｎｉｃｓ， Ｉｎｃ． 音響トランスデューサドライバ及びコントローラ
US12406749B2 (en) 2017-12-15 2025-09-02 The Broad Institute, Inc. Systems and methods for predicting repair outcomes in genetic engineering
JP2021506814A (ja) 2017-12-15 2021-02-22 デイナ ファーバー キャンサー インスティチュート，インコーポレイテッド 安定化ペプチドによって介在される標的タンパク質の分解
KR20210152597A (ko) 2017-12-22 2021-12-15 (주)지플러스생명과학 키메라 게놈 조작 분자 및 방법
US20230193242A1 (en) * 2017-12-22 2023-06-22 The Broad Institute, Inc. Cas12b systems, methods, and compositions for targeted dna base editing
IL309585B2 (en) 2017-12-22 2025-03-01 Fate Therapeutics Inc Enhanced immune effector cells and use thereof
US11994512B2 (en) 2018-01-04 2024-05-28 Massachusetts Institute Of Technology Single-cell genomic methods to generate ex vivo cell systems that recapitulate in vivo biology with improved fidelity
US20200362366A1 (en) 2018-01-12 2020-11-19 Basf Se Gene underlying the number of spikelets per spike qtl in wheat on chromosome 7a
US12509492B2 (en) 2018-01-19 2025-12-30 Duke University Genome engineering with CRISPR-Cas systems in eukaryotes
CN111742051A (zh) 2018-01-23 2020-10-02 基础科学研究院 延伸的单向导rna及其用途
US11220694B1 (en) * 2018-01-29 2022-01-11 Inari Agriculture, Inc. Rice cells and rice plants
US11926835B1 (en) 2018-01-29 2024-03-12 Inari Agriculture Technology, Inc. Methods for efficient tomato genome editing
US20210047653A1 (en) * 2018-01-30 2021-02-18 The University Of Memphis Research Foundation Compositions and methods for regulating a biological process
US12275951B2 (en) * 2018-02-06 2025-04-15 Ohio State Innovation Foundation Engineered guide RNA and uses thereof
EP3765612A4 (en) * 2018-03-12 2022-01-05 Nanjing Bioheng Biotech Co., Ltd MODIFIED CHEMERICAL GUIDE RNA AND ITS USES
DK3765615T3 (da) 2018-03-14 2023-08-21 Arbor Biotechnologies Inc Nye enzymer og systemer til målretning af crispr dna
US12195770B2 (en) 2018-03-14 2025-01-14 Arbor Biotechnologies, Inc. CRISPR DNA and RNA targeting enzymes and systems
EP3765608A4 (en) * 2018-03-15 2022-01-12 KSQ Therapeutics, Inc. GENE REGULATORY COMPOSITIONS AND METHODS FOR IMPROVED IMMUNOTHERAPY
CN108486146B (zh) * 2018-03-16 2021-02-19 中国农业科学院作物科学研究所 LbCpf1-RR突变体用于CRISPR/Cpf1系统在植物基因编辑中的应用
KR20240038811A (ko) * 2018-03-19 2024-03-25 리제너론 파마슈티칼스 인코포레이티드 CRISPR/Cas 시스템을 사용한 동물에서의 전사 조절
EP3546582A1 (en) 2018-03-26 2019-10-02 KWS SAAT SE & Co. KGaA Promoter activating elements
US10435662B1 (en) 2018-03-29 2019-10-08 Inscripta, Inc. Automated control of cell growth rates for induction and transformation
EP3775228A4 (en) * 2018-03-29 2022-02-23 Fate Therapeutics, Inc. ENHANCED IMMUNE EFFECTIVE CELLS AND THEIR USE
KR20210029707A (ko) 2018-04-05 2021-03-16 주노 쎄러퓨티크스 인코퍼레이티드 재조합 수용체를 발현하는 세포의 생산 방법 및 관련 조성물
EP3775237A1 (en) 2018-04-05 2021-02-17 Juno Therapeutics, Inc. T cells expressing a recombinant receptor, related polynucleotides and methods
US11786554B2 (en) 2018-04-12 2023-10-17 Precision Biosciences, Inc. Optimized engineered nucleases having specificity for the human T cell receptor alpha constant region gene
WO2019200004A1 (en) 2018-04-13 2019-10-17 Inscripta, Inc. Automated cell processing instruments comprising reagent cartridges
EP3784785A4 (en) * 2018-04-23 2022-02-16 Duke University DOWNREGULATION OF SNCA EXPRESSION BY TARGETED EDITING OF DNA METHYLATION
US10501738B2 (en) 2018-04-24 2019-12-10 Inscripta, Inc. Automated instrumentation for production of peptide libraries
US10557216B2 (en) 2018-04-24 2020-02-11 Inscripta, Inc. Automated instrumentation for production of T-cell receptor peptide libraries
US10858761B2 (en) 2018-04-24 2020-12-08 Inscripta, Inc. Nucleic acid-guided editing of exogenous polynucleotides in heterologous cells
EP3560330B1 (en) 2018-04-24 2022-06-15 KWS SAAT SE & Co. KGaA Plants with improved digestibility and marker haplotypes
US11957695B2 (en) 2018-04-26 2024-04-16 The Broad Institute, Inc. Methods and compositions targeting glucocorticoid signaling for modulating immune responses
CN109678939B (zh) * 2018-04-27 2022-03-04 四川大学华西医院 一种FnCpf1突变体
MA52533A (fr) 2018-04-27 2021-03-03 Iovance Biotherapeutics Inc Procédé en circuit fermé pour l'amplification et l'edition de gènes de lymphocytes d'infiltration des tumeurs et leurs utilisations en immunothérapie
KR20250134703A (ko) 2018-05-11 2025-09-11 빔 테라퓨틱스, 인크. 프로그래밍가능한 염기 편집기 시스템을 이용하여 병원성 아미노산을 치환하는 방법
EP3790964A4 (en) * 2018-05-11 2022-06-08 Beam Therapeutics, Inc. METHODS FOR REMOVAL OF PATHOGENIC MUTATIONS USING PROGRAMMABLE BASE EDITOR SYSTEMS
US12522812B2 (en) 2018-05-16 2026-01-13 Arbor Biotechnologies, Inc. CRISPR-associated systems and components
US12157760B2 (en) 2018-05-23 2024-12-03 The Broad Institute, Inc. Base editors and uses thereof
US11833225B2 (en) * 2018-05-24 2023-12-05 Crispr Therapeutics Ag Methods and compositions for efficient gene deletion
EP3801640A4 (en) 2018-05-30 2022-03-23 The Regents Of The University Of California GENE EDIT OF MONOGENIC DISEASES IN HUMAN HEMATOPOIETIC STEM CELLS - CORRECTION OF X-LINKED AGAMMAGLOBULINEMIA (XLA)
EP3575402A1 (en) * 2018-06-01 2019-12-04 Algentech SAS Gene targeting
US11866719B1 (en) 2018-06-04 2024-01-09 Inari Agriculture Technology, Inc. Heterologous integration of regulatory elements to alter gene expression in wheat cells and wheat plants
MA52739A (fr) * 2018-06-04 2021-04-21 Univ Copenhagen Endonucléases cpf1 mutantes
EP4512900A3 (en) 2018-06-05 2025-04-02 LifeEDIT Therapeutics, Inc. Rna-guided nucleases and active fragments and variants thereof and methods of use
US12048296B2 (en) 2018-06-07 2024-07-30 Korea Research Institute Of Bioscience And Biotechnology Transgenic mouse for aglycosylated antibody production and use of aglycosylated antibody produced therefrom
WO2019237035A1 (en) 2018-06-08 2019-12-12 Intellia Therapeutics, Inc. Compositions and methods for immunooncology
US10227576B1 (en) 2018-06-13 2019-03-12 Caribou Biosciences, Inc. Engineered cascade components and cascade complexes
US12227776B2 (en) 2018-06-13 2025-02-18 Caribou Biosciences, Inc. Engineered cascade components and cascade complexes
US12036240B2 (en) 2018-06-14 2024-07-16 The Broad Institute, Inc. Compositions and methods targeting complement component 3 for inhibiting tumor growth
EP3807300A1 (en) 2018-06-15 2021-04-21 KWS SAAT SE & Co. KGaA Methods for improving genome engineering and regeneration in plant
US12146142B2 (en) 2018-06-15 2024-11-19 KWS SAAT SE & Co. KGaA Methods for improving genome engineering and regeneration in plant II
WO2019237395A1 (zh) * 2018-06-16 2019-12-19 深圳市博奥康生物科技有限公司 一种应用CRISPR/Cas9系统靶向敲除人CD357基因的方法
WO2019237380A1 (zh) * 2018-06-16 2019-12-19 深圳市博奥康生物科技有限公司 一种基于慢病毒的 CRISPR/Cpf1 基因编辑载体及其应用
US20210269866A1 (en) 2018-06-26 2021-09-02 The Broad Institute, Inc. Crispr effector system based amplification methods, systems, and diagnostics
BR112020026246A2 (pt) 2018-06-26 2021-04-20 The Broad Institute Inc. composições, sistemas e métodos de amplificação com base em nickase dupla crispr
EP3521436A1 (en) 2018-06-27 2019-08-07 VIB vzw Complex breeding in plants
AU2019291918B2 (en) 2018-06-29 2025-06-12 Editas Medicine, Inc. Synthetic guide molecules, compositions and methods relating thereto
WO2020005383A1 (en) 2018-06-30 2020-01-02 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
US12522807B2 (en) 2018-07-09 2026-01-13 The Broad Institute, Inc. RNA programmable epigenetic RNA modifiers and uses thereof
WO2020018166A1 (en) 2018-07-16 2020-01-23 The Regents Of The University Of Michigan Nuclease-mediated nucleic acid modification
US12553045B2 (en) * 2018-07-16 2026-02-17 Arbor Biotechnologies, Inc. Crispr DNA targeting enzymes and systems
WO2020028729A1 (en) 2018-08-01 2020-02-06 Mammoth Biosciences, Inc. Programmable nuclease compositions and methods of use thereof
MX2021001578A (es) * 2018-08-09 2021-06-15 G Flas Life Sciences Proteina asociada a crispr novedosa y uso de la misma.
WO2020030984A2 (en) 2018-08-09 2020-02-13 G+Flas Life Sciences Compositions and methods for genome engineering with cas12a proteins
US10752874B2 (en) 2018-08-14 2020-08-25 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
US10532324B1 (en) 2018-08-14 2020-01-14 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
US11142740B2 (en) 2018-08-14 2021-10-12 Inscripta, Inc. Detection of nuclease edited sequences in automated modules and instruments
US12497611B2 (en) 2018-08-17 2025-12-16 Yale University Compositions and methods for high-throughput activation screening to boost T cell effector function
WO2020041380A1 (en) 2018-08-20 2020-02-27 The Broad Institute, Inc. Methods and compositions for optochemical control of crispr-cas9
AR116016A1 (es) * 2018-08-24 2021-03-25 Flagship Pioneering Innovations Vi Llc Métodos para fabricar paquetes mensajeros vegetales
CA3109956A1 (en) * 2018-08-24 2020-02-27 Flagship Pioneering Innovations Vi, Llc Methods and compositions for the modification of plants
CN112955540A (zh) 2018-08-30 2021-06-11 因思科瑞普特公司 在自动化模块和仪器中对经核酸酶经编辑的序列的改进的检测
US12545910B2 (en) 2018-08-31 2026-02-10 Inari Agriculture Technology, Inc. Compositions, systems, and methods for genome editing
US11479762B1 (en) 2018-08-31 2022-10-25 Inari Agriculture Technology, Inc. Compositions, systems, and methods for genome editing
US12534743B2 (en) 2018-08-31 2026-01-27 Inari Agriculture Technology, Inc. Compositions, systems, and methods for genome editing
WO2020051507A1 (en) 2018-09-06 2020-03-12 The Broad Institute, Inc. Nucleic acid assemblies for use in targeted delivery
CN109402115B (zh) * 2018-09-06 2024-02-02 广州普世利华科技有限公司 靶向Rett突变基因RNA的gRNA及Rett突变基因的检测方法、检测试剂盒
US12454694B2 (en) 2018-09-07 2025-10-28 Beam Therapeutics Inc. Compositions and methods for improving base editing
US12529041B2 (en) 2018-09-07 2026-01-20 Beam Therapeutics Inc. Compositions and methods for delivering a nucleobase editing system
EP3847251A1 (en) 2018-09-07 2021-07-14 Astrazeneca AB Compositions and methods for improved nucleases
EP3849565A4 (en) 2018-09-12 2022-12-28 Fred Hutchinson Cancer Research Center REDUCTION OF CD33 EXPRESSION TO SELECTIVELY PROTECT THERAPEUTIC CELLS
US11459552B2 (en) 2018-09-13 2022-10-04 The Board Of Regents Of The University Of Oklahoma Variant CAS12 proteins with improved dna cleavage selectivity and methods of use
WO2020055941A1 (en) * 2018-09-13 2020-03-19 Excision Biotherapeutics, Inc. Compositions and methods for excision with single grna
US12331320B2 (en) 2018-10-10 2025-06-17 The Research Foundation For The State University Of New York Genome edited cancer cell vaccines
EP3867380A2 (en) 2018-10-18 2021-08-25 Intellia Therapeutics, Inc. Compositions and methods for expressing factor ix
US11214781B2 (en) 2018-10-22 2022-01-04 Inscripta, Inc. Engineered enzyme
EP3870697A4 (en) 2018-10-22 2022-11-09 Inscripta, Inc. GMO ENZYMES
CN111100876A (zh) * 2018-10-25 2020-05-05 立沃生物科技（深圳）有限公司 CRISPR-Cas9特异性敲除FAH基因的方法及特异性sgRNA
US11407995B1 (en) 2018-10-26 2022-08-09 Inari Agriculture Technology, Inc. RNA-guided nucleases and DNA binding proteins
WO2020092453A1 (en) 2018-10-29 2020-05-07 The Broad Institute, Inc. Nucleobase editors comprising geocas9 and uses thereof
US20210388389A1 (en) 2018-10-30 2021-12-16 Yale University Compositions and methods for rapid and modular generation of chimeric antigen receptor t cells
JP2022513408A (ja) 2018-10-31 2022-02-07 ザイマージェン インコーポレイテッド Ｄｎａライブラリーの多重化決定論的アセンブリー
US20220333089A1 (en) * 2018-11-01 2022-10-20 The University Of Tokyo Split CPF1 Protein
US11434477B1 (en) 2018-11-02 2022-09-06 Inari Agriculture Technology, Inc. RNA-guided nucleases and DNA binding proteins
KR20210091213A (ko) 2018-11-05 2021-07-21 이오반스 바이오테라퓨틱스, 인크. 종양 침윤 림프구의 생성 방법 및 면역치료법에서 이의 용도
CA3118493A1 (en) 2018-11-05 2020-05-14 Iovance Biotherapeutics, Inc. Expansion of tils utilizing akt pathway inhibitors
US20230039976A1 (en) 2018-11-05 2023-02-09 Iovance Biotherapeutics, Inc. Selection of improved tumor reactive t-cells
JP7688575B2 (ja) 2018-11-05 2025-06-04 アイオバンス バイオセラピューティクス，インコーポレイテッド 抗ｐｄ－１抗体に対して不応性のｎｓｃｌｃ患者の治療
CN109385451B (zh) * 2018-11-07 2020-07-07 中国海洋大学 一种牡蛎CRISPR/Cas9基因编辑方法
AU2019377115A1 (en) * 2018-11-07 2021-05-20 Akouos, Inc. Use of adeno-associated viral vectors to correct gene defects/ express proteins in hair cells and supporting cells in the inner ear
SG11202104802RA (en) * 2018-11-08 2021-06-29 Arizona Board Of Regents On Behalf Arizona State Univ Synthetic immunomodulation with a crispr super-repressor in vivo
AU2019374883A1 (en) 2018-11-09 2021-05-20 Inari Agriculture Technology, Inc. RNA-guided nucleases and DNA binding proteins
US12165743B2 (en) 2018-11-09 2024-12-10 The Broad Institute, Inc. Compressed sensing for screening and tissue imaging
US12402610B2 (en) 2018-11-09 2025-09-02 The Broad Institute, Inc. Methods and compositions for modulating innate lymphoid cell pathogenic effectors
AU2019379160A1 (en) * 2018-11-14 2021-06-24 Massachusetts Institute Of Technology CRISPR system based droplet diagnostic systems and methods
WO2020098793A1 (zh) * 2018-11-15 2020-05-22 中国农业大学 CRISPR-Cas12a酶和系统
WO2020098772A1 (zh) * 2018-11-15 2020-05-22 中国农业大学 CRISPR-Cas12j酶和系统
CN109536506B (zh) * 2018-12-10 2022-03-22 潍坊科技学院 一种调控日本沼虾生长发育的类胰岛素受体基因及其应用
US11166996B2 (en) 2018-12-12 2021-11-09 Flagship Pioneering Innovations V, Inc. Anellovirus compositions and methods of use
CN109666662A (zh) * 2018-12-12 2019-04-23 广州普世利华科技有限公司 新型ScCas12a在核酸检测方面的应用
CN109680053A (zh) * 2018-12-12 2019-04-26 广州普世利华科技有限公司 新型SsCas12a蛋白在核酸检测方面的应用
CN109593743A (zh) * 2018-12-12 2019-04-09 广州普世利华科技有限公司 新型CRISPR/ScCas12a蛋白及其制备方法
WO2020124050A1 (en) 2018-12-13 2020-06-18 The Broad Institute, Inc. Tiled assays using crispr-cas based detection
CN113544266A (zh) 2018-12-17 2021-10-22 博德研究所 Crispr相关转座酶系统和其使用方法
US12264323B2 (en) 2018-12-17 2025-04-01 The Broad Institute, Inc. CRISPR CPF1 direct repeat variants
US20220193131A1 (en) 2018-12-19 2022-06-23 Iovance Biotherapeutics, Inc. Methods of Expanding Tumor Infiltrating Lymphocytes Using Engineered Cytokine Receptor Pairs and Uses Thereof
US11690362B2 (en) 2018-12-20 2023-07-04 Regeneran Pharmaceuticals, Inc. Nuclease-mediated repeat expansion
EP3898661A1 (en) 2018-12-21 2021-10-27 Precision BioSciences, Inc. Genetic modification of the hydroxyacid oxidase 1 gene for treatment of primary hyperoxaluria
IL312708A (en) 2018-12-27 2024-07-01 Lifeedit Therapeutics Inc Polypeptides useful for gene editing and methods of use
WO2020142754A2 (en) 2019-01-04 2020-07-09 Mammoth Biosciences, Inc. Programmable nuclease improvements and compositions and methods for nucleic acid amplification and detection
US11739156B2 (en) 2019-01-06 2023-08-29 The Broad Institute, Inc. Massachusetts Institute of Technology Methods and compositions for overcoming immunosuppression
WO2020154500A1 (en) 2019-01-23 2020-07-30 The Broad Institute, Inc. Supernegatively charged proteins and uses thereof
CN109799295A (zh) * 2019-01-28 2019-05-24 江南大学 一种用于秀丽隐杆线虫代谢组学分析的样品前处理方法
US12460230B2 (en) 2019-02-10 2025-11-04 The J. David Gladstone Institutes Modified mitochondrion and methods of use thereof
JP2022520783A (ja) 2019-02-12 2022-04-01 ウニヴェルシタ デリ ストゥディ ディ トレント Ｃａｓ１２ａガイドＲＮＡ分子およびその使用
AU2019429609B2 (en) * 2019-02-13 2025-09-25 Probiogen Ag Transposase with enhanced insertion site selection properties
US20220235347A1 (en) 2019-02-13 2022-07-28 Beam Therapeutics Inc. Compositions and methods for treating hemoglobinopathies
CN119896751A (zh) * 2019-02-13 2025-04-29 比姆医疗股份有限公司 使用腺苷酸脱氨酶碱基编辑器破坏疾病相关基因的剪接受体位点，包括用于治疗遗传性疾病
US20220249703A1 (en) * 2019-02-15 2022-08-11 Allen Institute Artificial expression constructs for selectively modulating gene expression in selected neuronal cell populations
JP7407830B2 (ja) * 2019-02-22 2024-01-04 インテグレーティッド ディーエヌエイ テクノロジーズ インコーポレーティッド Ｌａｃｈｎｏｓｐｉｒａｃｅａｅ ｂａｃｔｅｒｉｕｍ ＮＤ２００６のＣＡＳ１２Ａ変異型遺伝子およびそれらによってコードされるポリペプチド
US20220133795A1 (en) 2019-03-01 2022-05-05 Iovance Biotherapeutics, Inc. Expansion of Tumor Infiltrating Lymphocytes From Liquid Tumors and Therapeutic Uses Thereof
CN109975543B (zh) * 2019-03-01 2022-02-11 中国疾病预防控制中心传染病预防控制所 分枝杆菌Ku蛋白的应用
EP3935155A4 (en) * 2019-03-07 2022-11-23 The Regents of The University of California Crispr-cas effector polypeptides and methods of use thereof
US11053515B2 (en) 2019-03-08 2021-07-06 Zymergen Inc. Pooled genome editing in microbes
WO2020185590A1 (en) 2019-03-08 2020-09-17 Zymergen Inc. Iterative genome editing in microbes
CN113825834A (zh) 2019-03-11 2021-12-21 索伦托药业有限公司 使用rna引导的核酸内切酶进行dna构建体整合的改进工艺
WO2020186231A2 (en) 2019-03-14 2020-09-17 The Broad Institute, Inc. Crispr effector system based multiplex diagnostics
US12534714B2 (en) 2019-03-18 2026-01-27 The Broad Institute, Inc. Type VII CRISPR proteins and systems
SG11202108090XA (en) 2019-03-18 2021-08-30 Regeneron Pharma Crispr/cas dropout screening platform to reveal genetic vulnerabilities associated with tau aggregation
WO2020190932A1 (en) 2019-03-18 2020-09-24 Regeneron Pharmaceuticals, Inc. Crispr/cas screening platform to identify genetic modifiers of tau seeding or aggregation
MX2021011426A (es) 2019-03-19 2022-03-11 Broad Inst Inc Metodos y composiciones para editar secuencias de nucleótidos.
AU2020247900A1 (en) 2019-03-25 2021-11-04 Inscripta, Inc. Simultaneous multiplex genome editing in yeast
US11001831B2 (en) 2019-03-25 2021-05-11 Inscripta, Inc. Simultaneous multiplex genome editing in yeast
KR20240122580A (ko) 2019-04-03 2024-08-12 프리시젼 바이오사이언시스 인코포레이티드 마이크로RNA-적응 shRNA(shRNAmiR)를 포함하는 유전자-변형 면역 세포
AU2020256225B9 (en) 2019-04-03 2025-04-10 Regeneron Pharmaceuticals, Inc. Methods and compositions for insertion of antibody coding sequences into a safe harbor locus
WO2020206139A1 (en) 2019-04-04 2020-10-08 Regeneron Pharmaceuticals, Inc. Non-human animals comprising a humanized coagulation factor 12 locus
US11111504B2 (en) 2019-04-04 2021-09-07 Regeneron Pharmaceuticals, Inc. Methods for scarless introduction of targeted modifications into targeting vectors
US20220204994A1 (en) 2019-04-05 2022-06-30 Precision Biosciences, Inc. Methods of preparing populations of genetically-modified immune cells
US20220186263A1 (en) 2019-04-05 2022-06-16 Osaka University Method for producing knock-in cell
WO2020214842A1 (en) 2019-04-17 2020-10-22 The Broad Institute, Inc. Adenine base editors with reduced off-target effects
KR20220002997A (ko) * 2019-04-25 2022-01-07 플래그쉽 파이어니어링 이노베이션스 브이아이, 엘엘씨 식물 메신저 팩과 관련된 조성물 및 방법
CN110195102A (zh) * 2019-04-30 2019-09-03 广州普世利华科技有限公司 一种β地中海贫血基因分型方法
CN110396543A (zh) * 2019-04-30 2019-11-01 广州普世利华科技有限公司 一种肿瘤相关基因突变位点筛查方法
EP3966334A1 (en) 2019-05-10 2022-03-16 Basf Se Regulatory nucleic acid molecules for enhancing gene expression in plants
WO2020232029A1 (en) 2019-05-13 2020-11-19 Iovance Biotherapeutics, Inc. Methods and compositions for selecting tumor infiltrating lymphocytes and uses of the same in immunotherapy
UA128175C2 (uk) 2019-05-13 2024-04-24 Квс Саат Се Енд Ко. Кгаа Посухостійкість у кукурудзи
CN110172442B (zh) * 2019-05-16 2022-05-13 上海苹谱医疗科技有限公司 一种人诱导多潜能干细胞、构建方法及其用途
WO2020236972A2 (en) * 2019-05-20 2020-11-26 The Broad Institute, Inc. Non-class i multi-component nucleic acid targeting systems
CA3141159A1 (en) 2019-05-21 2020-11-26 Sangamo Therapeutics, Inc. Controlled transgene expression in regulatory t cells
AU2020278906A1 (en) 2019-05-23 2021-11-25 Nomad Bioscience Gmbh RNA viral RNA molecule for gene editing
AR118995A1 (es) 2019-05-25 2021-11-17 Kws Saat Se & Co Kgaa Mejorador de la inducción de haploides
CN114585741B (zh) * 2019-05-28 2026-01-06 奥科坦特公司 转录中继系统
GB201907733D0 (en) * 2019-05-31 2019-07-17 Governing Council Of The Univ Of Toronto Methods and compositions for multiplex gene editing
EP3801011A1 (en) 2019-06-04 2021-04-14 Regeneron Pharmaceuticals, Inc. Non-human animals comprising a humanized ttr locus with a beta-slip mutation and methods of use
WO2020247587A1 (en) 2019-06-06 2020-12-10 Inscripta, Inc. Curing for recursive nucleic acid-guided cell editing
AU2020289581B2 (en) * 2019-06-07 2024-11-21 Regeneron Pharmaceuticals, Inc. Non-human animals comprising a humanized albumin locus
CN114375334B (zh) 2019-06-07 2026-03-20 斯克里贝治疗公司 工程化CasX系统
CA3134097A1 (en) 2019-06-13 2020-12-17 Wim VRIEZEN Tomato plant producing fruit having improved ripening characteristics
CA3137765A1 (en) 2019-06-14 2020-12-17 Regeneron Pharmaceuticals, Inc. Models of tauopathy
US10907125B2 (en) 2019-06-20 2021-02-02 Inscripta, Inc. Flow through electroporation modules and instrumentation
EP3986909A4 (en) 2019-06-21 2023-08-02 Inscripta, Inc. Genome-wide rationally-designed mutations leading to enhanced lysine production in e. coli
MX2021014861A (es) 2019-06-25 2022-06-22 Inari Agriculture Tech Inc Edicion genomica de reparacion dependiente de homologia mejorada.
US10927385B2 (en) 2019-06-25 2021-02-23 Inscripta, Inc. Increased nucleic-acid guided cell editing in yeast
SG11202111943UA (en) 2019-07-02 2021-11-29 Hutchinson Fred Cancer Res Recombinant ad35 vectors and related gene therapy improvements
CN112168808B (zh) * 2019-07-02 2023-01-24 深圳市第二人民医院 一种基于crispr的细胞核靶向药物递送系统
JP7579817B2 (ja) * 2019-07-03 2024-11-08 インテグレーティッド ディーエヌエイ テクノロジーズ インコーポレーティッド Ｃｒｉｓｐｒで導入された二本鎖ｄｎａ切断修復の同定、特徴付けおよび定量化
EP3993612A1 (en) 2019-07-05 2022-05-11 Limagrain Europe Method for increasing yield in plants
JP2022541441A (ja) * 2019-07-17 2022-09-26 フェイト セラピューティクス，インコーポレイテッド 免疫エフェクター細胞操作およびその使用
US11746354B2 (en) 2019-07-19 2023-09-05 Inari Agriculture Technology, Inc. Homology dependent repair genome editing
WO2021016608A1 (en) 2019-07-25 2021-01-28 Precision Biosciences, Inc. Compositions and methods for sequential stacking of nucleic acid sequences into a genomic locus
WO2021021532A1 (en) * 2019-07-26 2021-02-04 Mammoth Biosciences, Inc. Compositions for detection of dna and methods of use thereof
WO2021022043A2 (en) 2019-07-30 2021-02-04 Pairwise Plants Services, Inc. Morphogenic regulators and methods of using the same
CN110358767B (zh) * 2019-07-30 2021-06-08 湖北大学 一种基于CRISPR-Cas12a系统的运动发酵单胞菌基因组编辑方法及其应用
US12371695B2 (en) 2019-08-02 2025-07-29 Monsanto Technology Llc Methods and compositions to promote targeted genome modifications using HUH endonucleases
EP3772542A1 (en) 2019-08-07 2021-02-10 KWS SAAT SE & Co. KGaA Modifying genetic variation in crops by modulating the pachytene checkpoint protein 2
WO2021028359A1 (en) 2019-08-09 2021-02-18 Sangamo Therapeutics France Controlled expression of chimeric antigen receptors in t cells
JP7640527B2 (ja) 2019-08-12 2025-03-05 ライフエディット セラピューティクス，インコーポレイティド Ｒｎａ誘導ヌクレアーゼ及びその活性断片及び変異体ならびに使用方法
CN110592014A (zh) * 2019-08-14 2019-12-20 广东美赛尔细胞生物科技有限公司 一种在nk细胞治疗中免辐照体外体内持续去除饲养细胞的方法
EP4047087A4 (en) * 2019-08-19 2023-08-23 Southern Medical University Construction of high-fidelity crispr/ascpf1 mutant and application thereof
CA3148179A1 (en) 2019-08-20 2021-02-25 Bruce J. Mccreedy Jr. Lymphodepletion dosing regimens for cellular immunotherapies
WO2021035170A1 (en) 2019-08-21 2021-02-25 Precision Biosciences, Inc. Compositions and methods for tcr reprogramming using fusion proteins
CN112442515B (zh) * 2019-09-02 2023-01-10 南京启真基因工程有限公司 gRNA靶点组合在构建血友病模型猪细胞系中的应用
US20220333208A1 (en) 2019-09-03 2022-10-20 The Broad Institute, Inc. Crispr effector system based multiplex cancer diagnostics
MX2022002642A (es) * 2019-09-05 2022-06-14 Benson Hill Inc Composiciones y metodos para modificar genomas.
AU2020341711A1 (en) * 2019-09-05 2022-03-03 Arbor Biotechnologies, Inc. Novel CRISPR DNA targeting enzymes and systems
MX2022002872A (es) 2019-09-09 2022-08-04 Arbor Biotechnologies Inc Nuevas sistemas y enzimas de crispr dirigidos a adn.
EP4028515A1 (en) 2019-09-10 2022-07-20 Science Solutions LLC Novel class 2 type ii and type v crispr-cas rna-guided endonucleases
CN110628816B (zh) * 2019-09-12 2022-10-18 中国医学科学院血液病医院(中国医学科学院血液学研究所) 一种用于血友病a的双切供体及其药物组合
CA3150334A1 (en) 2019-09-12 2021-03-18 Frank Meulewaeter Regulatory nucleic acid molecules for enhancing gene expression in plants
CA3153980A1 (en) 2019-09-13 2021-03-18 Regeneron Pharmaceuticals, Inc. Transcription modulation in animals using crispr/cas systems delivered by lipid nanoparticles
CN110600083B (zh) * 2019-09-20 2020-05-19 中国人民解放军军事科学院军事医学研究院 基于无拼接组装wgs数据的醋酸钙—鲍曼不动杆菌复合群鉴定方法
CA3147643A1 (en) 2019-09-23 2021-04-01 Omega Therapeutics, Inc. Compositions and methods for modulating hepatocyte nuclear factor 4-alpha (hnf4.alpha.) gene expression
EP4048807A1 (en) 2019-09-23 2022-08-31 Omega Therapeutics, Inc. Compositions and methods for modulating apolipoprotein b (apob) gene expression
CA3153197A1 (en) * 2019-10-03 2021-04-08 Ryan T. Gill Crispr systems with engineered dual guide nucleic acids
WO2021069387A1 (en) 2019-10-07 2021-04-15 Basf Se Regulatory nucleic acid molecules for enhancing gene expression in plants
US12435330B2 (en) 2019-10-10 2025-10-07 The Broad Institute, Inc. Methods and compositions for prime editing RNA
US20230357788A1 (en) 2019-10-17 2023-11-09 KWS SAAT SE & Co. KGaA Enhanced disease resistance of crops by downregulation of repressor genes
BR112022007125A2 (pt) * 2019-10-17 2022-07-05 Pairwise Plants Services Inc Variantes de nucleases cas12a e métodos de produção e uso das mesmas
WO2021081200A1 (en) 2019-10-22 2021-04-29 Inari Agriculture, Inc. Genomic alteration of plant germline
AU2020369599A1 (en) 2019-10-23 2022-05-12 Monsanto Technology Llc Compositions and methods for RNA-templated editing in plants
WO2021081384A1 (en) * 2019-10-25 2021-04-29 Greenvenus, Llc Synthetic nucleases
EP4048295A1 (en) 2019-10-25 2022-08-31 Iovance Biotherapeutics, Inc. Gene editing of tumor infiltrating lymphocytes and uses of same in immunotherapy
CA3158897A1 (en) 2019-10-30 2021-05-06 Pairwise Plants Services, Inc. Type v crispr-cas base editors and methods of use thereof
US20220411479A1 (en) 2019-10-30 2022-12-29 Precision Biosciences, Inc. Cd20 chimeric antigen receptors and methods of use for immunotherapy
US11844800B2 (en) 2019-10-30 2023-12-19 Massachusetts Institute Of Technology Methods and compositions for predicting and preventing relapse of acute lymphoblastic leukemia
US20230002746A1 (en) * 2019-10-31 2023-01-05 Inari Agriculture Technology, Inc. Base-editing systems
CA3160186A1 (en) 2019-11-05 2021-05-14 Pairwise Plants Services, Inc. Compositions and methods for rna-encoded dna-replacement of alleles
AU2020379046B2 (en) 2019-11-08 2025-03-13 Regeneron Pharmaceuticals, Inc. CRISPR and AAV strategies for X-linked juvenile retinoschisis therapy
US20230002453A1 (en) * 2019-11-18 2023-01-05 Shanghai Bluecross Medical Science Institute Gene editing system derived from flavobacteria
US11203762B2 (en) 2019-11-19 2021-12-21 Inscripta, Inc. Methods for increasing observed editing in bacteria
CN110760597B (zh) * 2019-11-22 2022-08-23 西北农林科技大学 一种检测黄牛ncstn基因拷贝数变异的方法及其应用
WO2021108363A1 (en) 2019-11-25 2021-06-03 Regeneron Pharmaceuticals, Inc. Crispr/cas-mediated upregulation of humanized ttr allele
EP4065701A4 (en) * 2019-11-27 2023-11-29 Danmarks Tekniske Universitet CONSTRUCTS, COMPOSITIONS AND METHODS THEREOF WITH IMPROVED GENOMEDITING EFFICIENCY AND SPECIFICITY
CA3163087A1 (en) * 2019-11-27 2021-06-03 The General Hospital Corporation System and method for activating gene expression
CN114945273B (zh) 2019-11-29 2025-08-12 巴斯夫欧洲公司 增加植物对抗真菌感染的抗性
AU2020396138A1 (en) 2019-12-03 2022-06-16 Basf Se Regulatory nucleic acid molecules for enhancing gene expression in plants
EP4069850A4 (en) * 2019-12-04 2024-03-27 Arbor Biotechnologies, Inc. COMPOSITIONS CONTAINING NUCLEASE AND USES THEREOF
WO2021113788A1 (en) * 2019-12-06 2021-06-10 Pairwise Plants Services, Inc. Recruitment methods and compounds, compositions and systems for recruitment
US20230036065A1 (en) 2019-12-06 2023-02-02 Precision Biosciences, Inc. Methods for cancer immunotherapy
CN114787347B (zh) 2019-12-10 2024-07-12 因思科瑞普特公司 新颖的mad核酸酶
JP7749561B2 (ja) 2019-12-11 2025-10-06 アイオバンス バイオセラピューティクス，インコーポレイテッド 腫瘍浸潤リンパ球（ｔｉｌ）の産生のためのプロセス及びそれを使用する方法
KR102493904B1 (ko) * 2019-12-13 2023-01-31 한국생명공학연구원 EeCpf1에 의해 IL2Rg 유전자가 돌연변이된 면역부전 동물모델 및 그 제조방법
RU2720769C1 (ru) * 2019-12-13 2020-05-13 Федеральное бюджетное учреждение науки «Центральный научно-исследовательский институт эпидемиологии» Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека (ФБУН ЦНИИ Эпидемиологии Роспотребнадзора) Способ получения препарата рибонуклеопротеинового комплекса CRISPR/CAS и препарат для детекции провирусной ДНК вируса иммунодефицита человека, интегрированной в геном человека, в ультранизких концентрациях
US10704033B1 (en) 2019-12-13 2020-07-07 Inscripta, Inc. Nucleic acid-guided nucleases
RU2720767C1 (ru) * 2019-12-13 2020-05-13 Федеральное бюджетное учреждение науки «Центральный научно-исследовательский институт эпидемиологии» Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека (ФБУН ЦНИИ Эпидемиологии Роспотребнадзора) Способ обнаружения провирусной ДНК вируса иммунодефицита человека, интегрированной в геном человека, в ультранизких концентрациях и специфические олигонуклеотиды для использования в способе
RU2720768C1 (ru) * 2019-12-13 2020-05-13 Федеральное бюджетное учреждение науки "Центральный научно-исследовательский институт эпидемиологии" Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека (ФБУН ЦНИИ Эпидемиологии Роспотребнадзора) Система CRISPR-Cas для детекции провирусной ДНК вируса иммунодефицита человека, интегрированной в геном человека, в ультранизких концентрациях
CN110791523B (zh) * 2019-12-13 2022-05-10 南京农业大学 一种棉花抗旱相关基因GhRCHY1及其应用
KR20220116173A (ko) 2019-12-16 2022-08-22 바스프 아그리컬쳐럴 솔루션즈 시드 유에스 엘엘씨 밀의 게놈에의 dna 또는 돌연변이의 정확한 도입
EP4077682A1 (en) 2019-12-16 2022-10-26 BASF Agricultural Solutions Seed US LLC Improved genome editing using paired nickases
CA3157127A1 (en) 2019-12-18 2021-06-24 Aamir MIR Cascade/dcas3 complementation assays for in vivo detection of nucleic acid-guided nuclease edited cells
CA3161898A1 (en) 2019-12-19 2021-06-24 Basf Se Increasing space-time-yield, carbon-conversion-efficiency and carbon substrate flexibility in the production of fine chemicals
BR112022011895A2 (pt) 2019-12-20 2022-09-06 Basf Se Diminuição da toxidez de terpenos e aumento da produção potencial em microrganismos
CN111073902B (zh) * 2019-12-30 2022-04-26 广东省微生物研究所（广东省微生物分析检测中心） 提升胶霉毒素生物合成基因表达水平的CRISPR/dCas9载体及其构建方法和应用
US20230203463A1 (en) 2019-12-30 2023-06-29 LifeEDIT Therapeutics, Inc. Rna-guided nucleases and active fragments and variants thereof and methods of use
CN113073118B (zh) * 2020-01-03 2023-09-15 珠海联邦制药股份有限公司 Tet-off表达调控系统在级联放大启动子活性中的用途
WO2021141970A1 (en) 2020-01-06 2021-07-15 Pairwise Plants Services, Inc. Recruitment of dna polymerase for templated editing
US10689669B1 (en) 2020-01-11 2020-06-23 Inscripta, Inc. Automated multi-module cell processing methods, instruments, and systems
US20230340537A1 (en) 2020-01-17 2023-10-26 The Broad Institute, Inc. Small type ii-d cas proteins and methods of use thereof
WO2021148447A1 (en) 2020-01-21 2021-07-29 Limagrain Europe Wheat haploid inducer plant and uses
WO2021151085A2 (en) 2020-01-24 2021-07-29 The General Hospital Corporation Crispr-cas enzymes with enhanced on-target activity
US12312613B2 (en) 2020-01-24 2025-05-27 The General Hospital Corporation Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants
WO2021151065A2 (en) * 2020-01-24 2021-07-29 The General Hospital Corporation Methods to characterize enzymes for genome engineering
US11225674B2 (en) 2020-01-27 2022-01-18 Inscripta, Inc. Electroporation modules and instrumentation
CA3169462A1 (en) 2020-01-30 2021-08-05 Pairwise Plants Services, Inc. Compositions, systems, and methods for base diversification
AR121242A1 (es) 2020-01-31 2022-05-04 Pairwise Plants Services Inc Supresión de la respuesta de escape a la sombra en plantas
US20230063560A1 (en) 2020-02-04 2023-03-02 Pairwise Plans Services, Inc. Thornless and/or prickleless rubus plants
CN111337666B (zh) * 2020-02-12 2021-04-02 山东大学 I-motif重组介导的FRET探针及其原位成像癌细胞表面蛋白质同源二聚化的应用
CN113261532A (zh) * 2020-02-17 2021-08-17 中山大学 一种肺癌动物模型的构建方法
WO2021165508A1 (en) 2020-02-21 2021-08-26 Biogemma Prime editing technology for plant genome engineering
CA3172333A1 (en) 2020-02-21 2021-08-26 Pairwise Plants Services, Inc. Improved resistance to soybean cyst nematode through gene editing
WO2021173612A1 (en) 2020-02-26 2021-09-02 Sorrento Therapeutics, Inc. Activatable antigen binding proteins with universal masking moieties
US12012433B1 (en) * 2020-02-27 2024-06-18 Integrated Dna Technologies Inc. Expression and purification of Cas enzymes
CA3169105A1 (en) 2020-02-28 2021-09-02 Ling MENG Method for rapid genome modification in recalcitrant plants
CA3169128A1 (en) 2020-02-28 2021-09-02 Ling MENG Immature inflorescence meristem editing
BR112022017070A2 (pt) * 2020-02-28 2022-11-16 Huigene Therapeutics Co Ltd Sistema crispr-cas tipo vi-e e tipo vi-f e usos do mesmo
JP2023515671A (ja) 2020-03-04 2023-04-13 リジェネロン・ファーマシューティカルズ・インコーポレイテッド 免疫療法に対する腫瘍細胞の感作のための方法及び組成物
GB2617658B (en) * 2020-03-06 2024-04-17 Metagenomi Inc Class II, type V CRISPR systems
EP4118207A1 (en) 2020-03-11 2023-01-18 Omega Therapeutics, Inc. Compositions and methods for modulating forkhead box p3 (foxp3) gene expression
EP3878958A1 (en) 2020-03-11 2021-09-15 B.R.A.I.N. Biotechnology Research And Information Network AG Crispr-cas nucleases from cpr-enriched metagenome
WO2021188734A1 (en) 2020-03-17 2021-09-23 The Broad Institute, Inc. Crispr system high throughput diagnostic systems and methods
WO2021195079A1 (en) 2020-03-23 2021-09-30 Regeneron Pharmaceuticals, Inc. Non-human animals comprising a humanized ttr locus comprising a v30m mutation and methods of use
US11999946B2 (en) 2020-03-26 2024-06-04 Pairwise Plants Services, Inc. Methods for controlling meristem size for crop improvement
US11882808B2 (en) 2020-03-27 2024-01-30 Pairwise Plants Services, Inc. Methods for improving resistance to soybean rust
US20230263121A1 (en) 2020-03-31 2023-08-24 Elo Life Systems Modulation of endogenous mogroside pathway genes in watermelon and other cucurbits
US20230175006A1 (en) 2020-04-06 2023-06-08 Pairwise Plants Services, Inc. Methods and compositions for increasing resistance to ear rot and stem rot disease in maize
EP4133069A2 (en) 2020-04-08 2023-02-15 Astrazeneca AB Compositions and methods for improved site-specific modification
RU2743861C1 (ru) * 2020-04-15 2021-03-01 Федеральное бюджетное учреждение науки "Центральный научно-исследовательский институт эпидемиологии" Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека (ФБУН ЦНИИ Эпидемиологии Роспотребнадзора) Система CRISPR-Cas для выявления гена антибиотикоустойчивости blaVIM-2 (металло-бета-лактамаза класс B VIM-2) Pseudomonas aeruginosa в ультранизких концентрациях
RU2745637C1 (ru) * 2020-04-15 2021-03-29 Федеральное бюджетное учреждение науки "Центральный научно-исследовательский институт эпидемиологии" Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека (ФБУН ЦНИИ Эпидемиологии Роспотребнадзора) Способ получения препарата рибонуклеопротеинового комплекса CRISPR/Cas и препарат для выявления гена антибиотикоустойчивости bla VIM-2 (металло-бета-лактамаза класс B VIM-2) Pseudomonas aeruginosa в ультранизких концентрациях
CA3180157A1 (en) 2020-04-16 2021-10-21 Pairwise Plants Services, Inc. Methods for controlling meristem size for crop improvement
US20210332388A1 (en) 2020-04-24 2021-10-28 Inscripta, Inc. Compositions, methods, modules and instruments for automated nucleic acid-guided nuclease editing in mammalian cells
US20230167191A1 (en) 2020-04-24 2023-06-01 Sorrento Therapeutics, Inc. Memory Dimeric Antigen Receptors (mDARs)
TW202208626A (zh) 2020-04-24 2022-03-01 美商生命編輯公司 Rna引導核酸酶及其活性片段與變體，以及使用方法
CN116096878A (zh) 2020-05-01 2023-05-09 合成Dna技术公司 在非经典tttt前间区序列邻近基序处具有增强的切割活性的毛螺菌科菌种cas12a突变体
CA3176826A1 (en) 2020-05-04 2021-11-11 Iovance Biotherapeutics, Inc. Processes for production of tumor infiltrating lymphocytes and uses of the same in immunotherapy
WO2021226558A1 (en) 2020-05-08 2021-11-11 The Broad Institute, Inc. Methods and compositions for simultaneous editing of both strands of a target double-stranded nucleotide sequence
WO2021224506A1 (en) * 2020-05-08 2021-11-11 Universitat Pompeu Fabra Crispr-cas homology directed repair enhancer
US20230183664A1 (en) 2020-05-11 2023-06-15 Precision Biosciences, Inc. Self-limiting viral vectors encoding nucleases
IL298021A (en) 2020-05-13 2023-01-01 Nunhems Bv A method for obtaining mutant plants by targeted mutagenesis
MX2022014185A (es) 2020-05-13 2022-12-07 Nunhems Bv Plantas de tomate con recombinacion meiotica suprimida.
US11787841B2 (en) 2020-05-19 2023-10-17 Inscripta, Inc. Rationally-designed mutations to the thrA gene for enhanced lysine production in E. coli
US20230175019A1 (en) * 2020-05-28 2023-06-08 University Of Southern California Scalable trio guide rna approach for integration of large donor dna
JP2023527446A (ja) 2020-05-29 2023-06-28 カー・ヴェー・エス ザート エス・エー ウント コー． カー・ゲー・アー・アー 植物単数体の誘導
BR112022024489A2 (pt) 2020-06-02 2023-01-31 Pairwise Plants Services Inc Métodos para o controle do tamanho do meristema para melhora da cultura agrícola
US20230357735A1 (en) * 2020-06-03 2023-11-09 Mammoth Biosciences, Inc. Programmable nucleases and methods of use
CN111808832B (zh) * 2020-06-05 2022-02-11 华南农业大学 一种水稻纹枯病菌阳离子转运ATP酶基因及其片段Rscta和应用
JP7419168B2 (ja) * 2020-06-10 2024-01-22 株式会社東芝 改変型ｐｉｇｇｙＢａｃトランスポゼースのポリペプチド、それをコードするポリヌクレオチド、導入キャリア、キット、細胞のゲノムに目的配列を組込む方法及び細胞製造方法
AU2021287968A1 (en) 2020-06-12 2023-02-09 Sherlock Biosciences, Inc. CRISPR-based SARS-CoV-2 detection
WO2021257775A1 (en) 2020-06-17 2021-12-23 Pairwise Plants Services, Inc. Methods for controlling meristem size for crop improvement
EP4171616A1 (en) 2020-06-26 2023-05-03 Juno Therapeutics GmbH Engineered t cells conditionally expressing a recombinant receptor, related polynucleotides and methods
WO2022002989A1 (en) 2020-06-29 2022-01-06 KWS SAAT SE & Co. KGaA Boosting homology directed repair in plants
AU2021300169A1 (en) 2020-06-30 2023-02-02 Pairwise Plants Services, Inc. Compositions, systems, and methods for base diversification
CA3173949A1 (en) 2020-07-15 2022-01-20 LifeEDIT Therapeutics, Inc. Uracil stabilizing proteins and active fragments and variants thereof and methods of use
CN111778252B (zh) * 2020-07-17 2022-01-07 湖北省农业科学院畜牧兽医研究所 一种靶向敲除SST基因的sgRNA及其CRISPR/Cas9系统和应用
EP3943600A1 (en) 2020-07-21 2022-01-26 B.R.A.I.N. Biotechnology Research And Information Network AG Novel, non-naturally occurring crispr-cas nucleases for genome editing
WO2022020407A1 (en) 2020-07-21 2022-01-27 Pairwise Plants Services, Inc. Optimized protein linkers and methods of use
EP4121557A4 (en) * 2020-08-06 2024-05-01 Singular Genomics Systems, Inc. SPATIAL SEQUENCING
US20260028412A1 (en) 2020-08-10 2026-01-29 Precision Biosciences, Inc. Antibodies and fragments specific for b-cell maturation antigen and uses thereof
CN116583607A (zh) * 2020-08-13 2023-08-11 耶鲁大学 用于工程改造和选择具有期望表型的car t细胞的组合物和方法
WO2022046669A2 (en) * 2020-08-24 2022-03-03 Crisp-Hr Therapeutics, Inc. A nuclease and application thereof
CN111979273B (zh) * 2020-08-24 2022-05-27 苏州启辰生物科技有限公司 一种制备人源化ace2小鼠模型的方法
JP2023539668A (ja) 2020-08-28 2023-09-15 ペアワイズ・プランツ・サーヴィシズ，インコーポレイテッド 操作されたＣＲＩＳＰＲ－Ｃａｓタンパク質およびその使用方法
CA3191396A1 (en) 2020-09-01 2022-03-10 Alexis ROVNER Mammalian cells and methods for engineering the same
EP4208545A4 (en) 2020-09-01 2024-10-30 The Board of Trustees of the Leland Stanford Junior University SYNTHETIC MINIATURE CRISPR-CAS SYSTEM (CASMINI) FOR EUKARYOTIC GENOME ENGINEERING
EP4208544A2 (en) 2020-09-02 2023-07-12 Massachusetts Institute of Technology Systems, methods, and compositions for rna-guided rna-targeting crispr effectors
JP7719172B2 (ja) 2020-09-11 2025-08-05 ライフ エディット セラピューティクス，インコーポレイティド Ｄｎａ修飾酵素並びにその活性断片及びバリアント並びにその使用方法
EP4214314A4 (en) 2020-09-15 2024-10-16 Inscripta, Inc. Crispr editing to embed nucleic acid landing pads into genomes of live cells
JP2023543351A (ja) 2020-09-18 2023-10-13 アーティザン ディヴェロップメント ラブス インコーポレイテッド 効率的かつ特異的なゲノム編集のためのコンストラクト及びその使用
WO2022067089A1 (en) 2020-09-25 2022-03-31 Beam Therapeutics Inc. Fratricide resistant modified immune cells and methods of using the same
WO2022188039A1 (en) 2021-03-09 2022-09-15 Huigene Therapeutics Co., Ltd. Engineered crispr/cas13 system and uses thereof
WO2022068912A1 (en) 2020-09-30 2022-04-07 Huigene Therapeutics Co., Ltd. Engineered crispr/cas13 system and uses thereof
US20230372397A1 (en) 2020-10-06 2023-11-23 Iovance Biotherapeutics, Inc. Treatment of nsclc patients with tumor infiltrating lymphocyte therapies
WO2022076606A1 (en) 2020-10-06 2022-04-14 Iovance Biotherapeutics, Inc. Treatment of nsclc patients with tumor infiltrating lymphocyte therapies
WO2022076547A1 (en) 2020-10-07 2022-04-14 Precision Biosciences, Inc. Lipid nanoparticle compositions
US20240060079A1 (en) 2020-10-23 2024-02-22 Elo Life Systems Methods for producing vanilla plants with improved flavor and agronomic production
WO2022090224A1 (en) 2020-10-27 2022-05-05 KWS SAAT SE & Co. KGaA Use of enhanced pol theta activity for eukaryotic genome engineering
CN112300999A (zh) * 2020-11-02 2021-02-02 江苏集萃药康生物科技股份有限公司 一种balb/c小鼠模型及其用途
EP4240853A2 (en) * 2020-11-03 2023-09-13 Science Solutions LLC Novel class 2 crispr-cas rna-guided endonucleases
US20230416709A1 (en) 2020-11-06 2023-12-28 Editforce, Inc. Foki nuclease domain mutant
AU2021374974A1 (en) 2020-11-06 2023-05-25 Pairwise Plants Services, Inc. Compositions and methods for rna-encoded dna-replacement of alleles
US11512297B2 (en) 2020-11-09 2022-11-29 Inscripta, Inc. Affinity tag for recombination protein recruitment
CA3198105A1 (en) * 2020-11-11 2022-05-19 X. Shawn Liu Multiplex epigenome editing
EP4243608A1 (en) 2020-11-11 2023-09-20 Leibniz-Institut für Pflanzenbiochemie (IPB) Fusion protein for editing endogenous dna of a eukaryotic cell
CN112410355B (zh) * 2020-11-23 2022-03-25 昆明理工大学 一种酰基辅酶a氧化酶2基因rkacox2及其应用
US20230416765A1 (en) 2020-11-23 2023-12-28 Pairwise Plants Services, Inc Agrobacterium rhizogenes and methods of transforming cells
CN112461807B (zh) * 2020-11-26 2021-11-19 山西大学 碳量子点在靶向细胞核仁免洗成像中的应用
WO2022120022A1 (en) 2020-12-02 2022-06-09 Regeneron Pharmaceuticals, Inc. Crispr sam biosensor cell lines and methods of use thereof
EP4256054A1 (en) 2020-12-03 2023-10-11 Scribe Therapeutics Inc. Engineered class 2 type v crispr systems
CN112195164B (zh) * 2020-12-07 2021-04-23 中国科学院动物研究所 工程化的Cas效应蛋白及其使用方法
CN114634928B (zh) * 2020-12-15 2023-08-04 四川大学华西医院 一种降低stat3转录功能的核酸片段及其制药用途
WO2022133149A1 (en) 2020-12-17 2022-06-23 Iovance Biotherapeutics, Inc. Treatment of cancers with tumor infiltrating lymphocytes
EP4263820A4 (en) * 2020-12-17 2025-03-26 Monsanto Technology LLC SSDNASE-FREE CRISPR ENDONUCLEASES
WO2022133140A1 (en) 2020-12-17 2022-06-23 Iovance Biotherapeutics, Inc. Treatment with tumor infiltrating lymphocyte therapies in combination with ctla-4 and pd-1 inhibitors
EP4019639A1 (en) 2020-12-22 2022-06-29 KWS SAAT SE & Co. KGaA Promoting regeneration and transformation in beta vulgaris
CA3206361A1 (en) 2020-12-23 2022-06-30 Flagship Pioneering Innovations V, Inc. In vitro assembly of anellovirus capsids enclosing rna
CN112553215A (zh) * 2020-12-24 2021-03-26 山东农业大学 一个玉米纹枯病抗病相关基因ZmFAH49及其应用
CN112662670B (zh) * 2020-12-25 2024-04-02 河南省农业科学院 花生脂酰-酰基载体蛋白硫酯酶AhFATB2基因启动子及其制备方法和应用
CN112640847B (zh) * 2020-12-30 2022-12-13 重庆医科大学附属第一医院 一种内源性癫痫发作动物模型及其构建方法
WO2022146497A1 (en) 2021-01-04 2022-07-07 Inscripta, Inc. Mad nucleases
US20240376451A1 (en) 2021-01-07 2024-11-14 Inscripta, Inc. Mad nucleases
KR20230142500A (ko) 2021-01-12 2023-10-11 마치 테라퓨틱스, 인크. 콘텍스트-의존적, 이중-가닥 dna-특이적 데아미나제및 이의 용도
CN112680479A (zh) * 2021-01-13 2021-04-20 汪利平 Cyp1b1基因缺失斑马鱼的制备
CN114790456A (zh) * 2021-01-24 2022-07-26 东北林业大学 野菊u6基因及其应用
WO2022159892A1 (en) * 2021-01-25 2022-07-28 The Broad Institute, Inc. Reprogrammable tnpb polypeptides and use thereof
WO2022165111A1 (en) 2021-01-28 2022-08-04 Precision Biosciences, Inc. Modulation of tgf beta signaling in genetically-modified eukaryotic cells
TW202241508A (zh) 2021-01-29 2022-11-01 美商艾歐凡斯生物治療公司 細胞介素相關之腫瘤浸潤性淋巴球組合物及方法
CN112852745B (zh) * 2021-02-04 2023-05-23 中国农业科学院兰州兽医研究所 Hdac3基因敲除的bhk-21细胞系及其构建方法和应用
CN112877275B (zh) * 2021-02-04 2023-03-31 中国农业科学院兰州兽医研究所 Hdac2基因敲除的bhk-21细胞系及其构建方法和应用
CN116004572B (zh) * 2021-02-05 2025-10-28 山东舜丰生物科技有限公司 Crispr酶和系统以及应用
EP4288112A4 (en) 2021-02-07 2025-08-06 Huidagene Therapeutics Singapore Pte Ltd RNA ADENO-ASSOCIATED VIRUS (ARAV) VECTOR AND USES THEREOF
EP4291678A1 (en) 2021-02-09 2023-12-20 Sherlock Biosciences, Inc. Nucleic acid amplification using promoter primers
MX2023009468A (es) 2021-02-11 2024-01-31 Pairwise Plants Services Inc Metodos y composiciones para modificar niveles de citoquinina oxidasa en plantas.
US11884924B2 (en) 2021-02-16 2024-01-30 Inscripta, Inc. Dual strand nucleic acid-guided nickase editing
WO2022175878A1 (en) * 2021-02-20 2022-08-25 Kcat Enzymatic Private Limited Insilico guided crispr-cas driven enzyme engineering framework
EP4298212A1 (en) 2021-02-25 2024-01-03 Artisan Development Labs, Inc. Compositions and methods for targeting, editing, or modifying genes
EP4298118A1 (en) 2021-02-25 2024-01-03 Pairwise Plants Services, Inc. Methods and compositions for modifying root architecture in plants
AU2022228662A1 (en) 2021-03-02 2023-08-24 BRAIN Biotech AG NOVEL CRISPR-Cas NUCLEASES FROM METAGENOMES
US20240108654A1 (en) 2021-03-03 2024-04-04 Juno Therapeutics, Inc. Combination of a t cell therapy and a dgk inhibitor
CA3211223A1 (en) * 2021-03-09 2022-09-15 Shaorong Chong Compositions comprising a variant polypeptide and uses thereof
CN115427561B (zh) 2021-03-09 2024-06-04 辉大(上海)生物科技有限公司 工程化CRISPR/Cas13系统及其用途
CN113005147A (zh) * 2021-03-11 2021-06-22 中山大学附属第一医院 一种usp8突变的小鼠动物模型的构建方法及其应用
US20240191191A1 (en) 2021-03-19 2024-06-13 Iovance Biotherapeutics, Inc. Methods for infiltrating lymphocyte (til) expansion related to cd39/cd69 selection and gene knockout in tils
CA3173950A1 (en) 2021-03-22 2022-09-22 Tyson D. BOWEN Dna modifyng enzymes and active fragments and variants thereof and methods of use
KR20240032711A (ko) 2021-03-25 2024-03-12 이오반스 바이오테라퓨틱스, 인크. T-세포 공배양 효능 검정 및 세포 치료제와 함께 사용하기 위한 방법 및 조성물
WO2022214522A2 (en) 2021-04-07 2022-10-13 Astrazeneca Ab Compositions and methods for site-specific modification
WO2022217086A1 (en) 2021-04-09 2022-10-13 Vor Biopharma Inc. Photocleavable guide rnas and methods of use thereof
EP4323501A1 (en) 2021-04-16 2024-02-21 Beam Therapeutics Inc. Genetic modification of hepatocytes
JP2024515189A (ja) 2021-04-19 2024-04-05 アイオバンス バイオセラピューティクス，インコーポレイテッド 細胞免疫療法におけるキメラ共刺激受容体、ケモカイン受容体、及びそれらの使用
KR102428773B1 (ko) * 2021-04-21 2022-08-05 (주)지플러스생명과학 분리형 crRNA 및 이를 이용한 CRISPR-Cas 시스템
US20240141311A1 (en) 2021-04-22 2024-05-02 North Carolina State University Compositions and methods for generating male sterile plants
WO2022236147A1 (en) 2021-05-06 2022-11-10 Artisan Development Labs, Inc. Modified nucleases
WO2022245754A1 (en) 2021-05-17 2022-11-24 Iovance Biotherapeutics, Inc. Pd-1 gene-edited tumor infiltrating lymphocytes and uses of same in immunotherapy
WO2022243437A1 (en) 2021-05-19 2022-11-24 KWS SAAT SE & Co. KGaA Sample preparation with oppositely oriented guide polynucleotides
CN113287569B (zh) * 2021-05-27 2023-04-07 四川康城生物科技有限公司 一种免疫低下动物模型的构建方法
US20220389418A1 (en) * 2021-06-01 2022-12-08 Helmholtz-Institut Für Rna-Basierte Nfektionsforschung (Hzi) Rna-guided cas nucleases and uses thereof in diagnostics and therapy
EP4419672A2 (en) 2021-06-01 2024-08-28 Artisan Development Labs, Inc. Compositions and methods for targeting, editing, or modifying genes
EP4347818A2 (en) 2021-06-01 2024-04-10 Arbor Biotechnologies, Inc. Gene editing systems comprising a crispr nuclease and uses thereof
CN113064220A (zh) * 2021-06-03 2021-07-02 四川九通智路科技有限公司 基于非线性自回归神经网络的能见度测量系统及测量方法
US20240271162A1 (en) 2021-06-11 2024-08-15 LifeEDIT Therapeutics, Inc. Rna polymerase iii promoters and methods of use
WO2022265905A2 (en) 2021-06-14 2022-12-22 Pairwise Plants Services, Inc. Reporter constructs, compositions comprising the same, and methods of use thereof
US20240279719A1 (en) * 2021-06-17 2024-08-22 The Board Of Trustees Of The University Of Illinois Crispr cascade
UY39822A (es) 2021-06-17 2022-12-30 Pairwise Plants Services Inc Modificación de factores de transcripción de la familia de factores reguladores del crecimiento en s
EP4370676A2 (en) 2021-06-18 2024-05-22 Artisan Development Labs, Inc. Compositions and methods for targeting, editing or modifying human genes
UY39827A (es) 2021-06-24 2023-01-31 Pairwise Plants Services Inc Modificación de genes de ubiquitina ligasa e3 hect para mejorar los rasgos de rendimiento
WO2023278651A1 (en) 2021-07-01 2023-01-05 Pairwise Plants Services, Inc. Methods and compositions for enhancing root system development
EP4368713A4 (en) * 2021-07-05 2025-10-08 Genkore Inc CLEAVAGE-INACTIVE CAS12F1, CLEAVAGE-INACTIVE CAS12F1-BASED FUSION PROTEIN, CRISPR GENE EDITING SYSTEM COMPRISING SAME, PREPARATION METHOD AND USE THEREOF
CN117987554A (zh) * 2021-07-06 2024-05-07 苏州淦江生物技术有限公司 一种基于Cas蛋白的检测方法
WO2023283585A2 (en) 2021-07-06 2023-01-12 Vor Biopharma Inc. Inhibitor oligonucleotides and methods of use thereof
WO2023283359A2 (en) 2021-07-07 2023-01-12 Omega Therapeutics, Inc. Compositions and methods for modulating secreted frizzled receptor protein 1 (sfrp1) gene expression
CN113383747B (zh) * 2021-07-09 2022-12-27 广西贺州农贝贝农牧科技有限公司 一种产蛋后期老龄鸡强制换羽的工艺方法
WO2023283636A1 (en) * 2021-07-09 2023-01-12 Acrigen Biosciences Compositions and methods for nucleic acid modifications
WO2023287669A2 (en) 2021-07-12 2023-01-19 Labsimply, Inc. Nuclease cascade assay
CA3226111A1 (en) 2021-07-22 2023-01-26 Iovance Biotherapeutics, Inc. Method for cryopreservation of solid tumor fragments
EP4377446A1 (en) 2021-07-28 2024-06-05 Iovance Biotherapeutics, Inc. Treatment of cancer patients with tumor infiltrating lymphocyte therapies in combination with kras inhibitors
CA3221517A1 (en) 2021-07-30 2023-02-02 Monika KLOIBER-MAITZ Plants with improved digestibility and marker haplotypes
WO2023015182A1 (en) 2021-08-02 2023-02-09 Vor Biopharma Inc. Compositions and methods for gene modification
WO2023019188A1 (en) 2021-08-12 2023-02-16 Pairwise Plants Services, Inc. Modification of brassinosteroid receptor genes to improve yield traits
WO2023020938A1 (en) 2021-08-16 2023-02-23 Nunhems B.V. Lettuce plant having delayed bolting
PY2270221A (es) 2021-08-17 2023-03-20 Pairwise Plants Services Inc Métodos y composiciones para modificar genes de histidina quinasa receptores de citoquinina en plantas
WO2023028444A1 (en) * 2021-08-27 2023-03-02 Mammoth Biosciences, Inc. Effector proteins and methods of use
US12359215B2 (en) 2021-08-30 2025-07-15 Pairwise Plants Services, Inc. Modification of ubiquitin binding peptidase genes in plants for yield trait improvement
CN113584077B (zh) * 2021-08-31 2023-09-15 西南大学 沉默桑实杯盘菌关键致病基因CsGPA1的沉默载体及其应用和方法
AR126938A1 (es) 2021-09-02 2023-11-29 Pairwise Plants Services Inc Métodos y composiciones para mejorar la arquitectura de las plantas y los rasgos de rendimiento
US20230266293A1 (en) 2021-09-21 2023-08-24 Pairwise Plants Services, Inc. Color-based and/or visual methods for identifying the presence of a transgene and compositions and constructs relating to the same
CN118382634A (zh) 2021-09-21 2024-07-23 成对植物服务股份有限公司 用于减少油菜荚果开裂的方法和组合物
CN118284701A (zh) 2021-09-27 2024-07-02 孟山都技术公司 用于转化从单子叶植物种子切除的胚外植体的组合物和方法
EP4408991A2 (en) 2021-09-27 2024-08-07 Vor Biopharma Inc. Fusion polypeptides for genetic editing and methods of use thereof
WO2023052508A2 (en) 2021-09-30 2023-04-06 Astrazeneca Ab Use of inhibitors to increase efficiency of crispr/cas insertions
US20230108968A1 (en) 2021-10-04 2023-04-06 Pairwise Plants Services, Inc. Methods for improving floret fertility and seed yield
WO2023059115A1 (ko) * 2021-10-06 2023-04-13 주식회사 진코어 유전자 편집을 위한 target 시스템 및 이의 용도
CA3234455A1 (en) 2021-10-07 2023-04-13 Pairwise Plants Services, Inc. Methods for improving floret fertility and seed yield
WO2023064872A1 (en) 2021-10-14 2023-04-20 Precision Biosciences, Inc. Combinations of anti-bcma car t cells and gamma secretase inhibitors
EP4166670A1 (en) 2021-10-18 2023-04-19 KWS SAAT SE & Co. KGaA Plant-tag-based weeding control
US20240408235A1 (en) 2021-10-19 2024-12-12 Precision Biosciences, Inc. Gene editing methods for treating alpha-1 antitrypsin (aat) deficiency
CN116024254A (zh) * 2021-10-26 2023-04-28 成都极谷基因科技有限公司 编辑水稻基因组中OsGBSSⅠ启动子及内含子1以提高稻米品质的方法
AR127482A1 (es) 2021-10-27 2024-01-31 Iovance Biotherapeutics Inc Sistemas y métodos para coordinar la fabricación de células para inmunoterapia específica de paciente
EP4423271A2 (en) 2021-10-28 2024-09-04 Regeneron Pharmaceuticals, Inc. Crispr/cas-related methods and compositions for knocking out c5
WO2023077148A1 (en) 2021-11-01 2023-05-04 Tome Biosciences, Inc. Single construct platform for simultaneous delivery of gene editing machinery and nucleic acid cargo
WO2023081900A1 (en) 2021-11-08 2023-05-11 Juno Therapeutics, Inc. Engineered t cells expressing a recombinant t cell receptor (tcr) and related systems and methods
CA3237410A1 (en) 2021-11-10 2023-05-19 Friedrich Graf Finck VON FINCKENSTEIN Methods of expansion treatment utilizing cd8 tumor infiltrating lymphocytes
US20250009800A1 (en) 2021-11-16 2025-01-09 Precision Biosciences, Inc. Methods for cancer immunotherapy
US20250025572A1 (en) * 2021-11-29 2025-01-23 Editas Medicine, Inc. Engineered crispr/cas12a effector proteins, and uses thereof
CN114404429B (zh) * 2021-11-30 2023-06-30 重庆医科大学附属第二医院 一种纳米银修饰的单宁酸-铁网络的载药纳米复合物及其制备方法和逆转肿瘤耐药应用
KR20240117571A (ko) 2021-12-08 2024-08-01 리제너론 파마슈티칼스 인코포레이티드 돌연변이 마이오실린 질환 모델 및 이의 용도
UY40060A (es) 2021-12-09 2023-07-14 Pairwise Plants Services Inc Métodos para mejorar la fertilidad de floretes y el rendimiento de semillas
WO2023114090A2 (en) * 2021-12-13 2023-06-22 Labsimply, Inc. Signal boost cascade assay
WO2023114750A1 (en) 2021-12-13 2023-06-22 Pairwise Plants Services, Inc. Model editing systems and methods relating to the same
WO2023114052A1 (en) 2021-12-13 2023-06-22 Labsimply, Inc. Tuning cascade assay kinetics via molecular design
US20250304982A1 (en) 2021-12-21 2025-10-02 Benson Hill, Inc. Compositions and methods for modifying genomes
AU2022420615A1 (en) 2021-12-22 2024-07-04 Tome Biosciences, Inc. Co-delivery of a gene editor construct and a donor template
AU2022417615A1 (en) 2021-12-23 2024-06-27 University Of Massachusetts Therapeutic treatment for fragile x-associated disorder
CA3246441A1 (en) 2022-01-06 2023-07-13 Pairwise Plants Services, Inc. METHODS AND COMPOSITIONS FOR THE ELIMINATION OF TRICHOMES
WO2023135524A1 (en) * 2022-01-12 2023-07-20 Genecker Co., Ltd. Cas9 proteins with enhanced specificity and uses thereof
AU2023209737B2 (en) * 2022-01-21 2026-02-19 Dong-A St Co., Ltd. Composition for improving intestinal health and function comprising milk-derived exosomes as active ingredient, and manufacturing method thereof
AU2023208961A1 (en) 2022-01-24 2024-09-12 Life Edit Therapeutics, Inc. Rna-guided nucleases and active fragments and variants thereof and methods of use
US20250099588A1 (en) 2022-01-28 2025-03-27 Iovance Biotherapeutics, Inc. Cytokine associated tumor infiltrating lymphocytes compositions and methods
UY40132A (es) 2022-01-31 2023-08-31 Pairwise Plants Services Inc Supresión de la respuesta de evitación de la sombra en las plantas
JP2025508677A (ja) 2022-02-02 2025-04-10 リジェネロン・ファーマシューティカルズ・インコーポレイテッド ポンペ病の処置のための抗ＴｆＲ：ＧＡＡ及び抗ＣＤ６３：ＧＡＡの挿入
WO2023154887A1 (en) 2022-02-11 2023-08-17 Northeast Agricultural University Methods and compositions for increasing protein and/or oil content and modifying oil profile in a plant
JP2025508723A (ja) * 2022-02-18 2025-04-10 プルモバイオティクス，エス．エル． 細菌を操作するためのシステム、ツール、及び方法
US20230383271A1 (en) 2022-02-28 2023-11-30 Pairwise Plants Services, Inc. Engineered proteins and methods of use thereof
CN119137268A (zh) 2022-03-01 2024-12-13 埃皮克里斯皮尔生物技术公司 工程化核酸酶、组合物及其使用方法
US20250388896A1 (en) 2022-03-01 2025-12-25 Celyntra Therapeutics Sa Composition and methods for transgene insertion
UY40171A (es) 2022-03-02 2023-09-29 Pairwise Plants Services Inc Modificación de genes del receptor de brasinoesteroides para mejorar rasgos de rendimiento
CN114271237B (zh) * 2022-03-07 2022-05-27 南京农业大学 一种长短绒自别雌雄鸽的培育方法
WO2023169410A1 (zh) 2022-03-08 2023-09-14 中国科学院遗传与发育生物学研究所 胞嘧啶脱氨酶及其在碱基编辑中的用途
CN114592023B (zh) * 2022-03-31 2023-03-24 杭州优玛达生物科技有限公司 一种细胞裂解自组装多肽复合物、自组装方法、自组装多肽制剂及应用
WO2023192838A1 (en) 2022-03-31 2023-10-05 Pairwise Plants Services, Inc. Early flowering rosaceae plants with improved characteristics
CA3247638A1 (en) 2022-04-06 2023-10-12 Maria Fardis TREATMENT OF PATIENTS WITH NON-SMALL CELL LUNG CARCINOMA (NSCLC) WITH TUMOR-INFILTRATING LYMPHOCYTE THERAPIES
AU2023251095A1 (en) 2022-04-07 2024-10-17 Monsanto Technology Llc Methods and compositions for improving resistance to fusarium head blight
CA3248034A1 (en) 2022-04-15 2023-10-19 Iovance Biotherapeutics, Inc. METHODS FOR EXPANSION OF TIL CELLS BY MEANS OF SPECIFIC CYTOKINE COMBINATIONS AND/OR AKT INHIBITOR TREATMENT
WO2023205744A1 (en) 2022-04-20 2023-10-26 Tome Biosciences, Inc. Programmable gene insertion compositions
CN119278208A (zh) 2022-04-21 2025-01-07 成对植物服务股份有限公司 用于改善产量性状的方法和组合物
EP4514981A2 (en) 2022-04-29 2025-03-05 Regeneron Pharmaceuticals, Inc. Identification of tissue-specific extragenic safe harbors for gene therapy approaches
UY40250A (es) 2022-05-02 2023-11-15 Pairwise Plants Services Inc Métodos y composiciones para mejorar el rendimiento y la resistencia a enfermedades
WO2023215831A1 (en) 2022-05-04 2023-11-09 Tome Biosciences, Inc. Guide rna compositions for programmable gene insertion
UY40255A (es) 2022-05-05 2023-11-15 Pairwise Plants Services Inc Métodos y composiciones para modificar la arquitectur radicular y/o mejorar los rangos de rendimient
WO2023220603A1 (en) 2022-05-09 2023-11-16 Regeneron Pharmaceuticals, Inc. Vectors and methods for in vivo antibody production
CA3251533A1 (en) 2022-05-10 2023-11-16 Iovance Biotherapeutics, Inc. TREATMENT OF CANCER PATIENTS WITH TUMOR-INFILTRATING LYMPHOCYTE THERAPIES IN COMBINATION WITH AN IL-15R AGONIST
WO2023225670A2 (en) 2022-05-20 2023-11-23 Tome Biosciences, Inc. Ex vivo programmable gene insertion
EP4526884A2 (en) 2022-05-20 2025-03-26 Artisan Development Labs, Inc. Systems and methods for assessing risk of genome editing events
CA3254044A1 (en) 2022-05-25 2023-11-30 Flagship Pioneering Innovations Vii, Llc COMPOSITIONS AND MODULATION METHODS OF TUMOR SUPPRESSANTS AND ONCOGENS
US20250319115A1 (en) 2022-05-25 2025-10-16 Flagship Pioneering Innovations Vii, Llc Compositions and Methods for Modulating Cytokines
CN114934031B (zh) 2022-05-25 2023-08-01 广州瑞风生物科技有限公司 新型Cas效应蛋白、基因编辑系统及用途
WO2023230498A1 (en) 2022-05-25 2023-11-30 Massachusetts Institute Of Technology Systems, methods, and compositions for rna-guided rna-targeting crispr effectors with cas7-11 variants
JP2025522292A (ja) 2022-05-25 2025-07-15 メタゲノミ，インク． 肝酵素発現の補充
EP4533088A2 (en) 2022-05-25 2025-04-09 Flagship Pioneering Innovations VII, LLC Compositions and methods for modulating genetic drivers
US20250320254A1 (en) 2022-05-25 2025-10-16 Flagship Pioneering Innovations Vii, Llc Compositions and Methods for Modulating Circulating Factors
EP4532765A2 (en) 2022-05-25 2025-04-09 Flagship Pioneering Innovations VII, LLC Compositions and methods for modulation of immune responses
EP4532720A2 (en) 2022-05-31 2025-04-09 Regeneron Pharmaceuticals, Inc. Crispr interference therapeutics for c9orf72 repeat expansion disease
WO2023235725A2 (en) 2022-05-31 2023-12-07 Regeneron Pharmaceuticals, Inc. Crispr-based therapeutics for c9orf72 repeat expansion disease
EP4534683A1 (en) 2022-06-01 2025-04-09 Institute Of Genetics And Developmental Biology, Chinese Academy Of Sciences Novel crispr gene editing system
EP4544051A2 (en) 2022-06-24 2025-04-30 Tune Therapeutics, Inc. Compositions, systems, and methods for reducing low-density lipoprotein through targeted gene repression
UY40326A (es) 2022-06-27 2023-12-29 Pairwise Plants Services Inc Métodos y composiciones para modificar el escape a la sombra en plantas
UY40337A (es) 2022-06-29 2023-12-29 Pairwise Plants Services Inc Métodos y composiciones para controlar el tamaño del meristemo para el mejoramiento de cultivos
UY40336A (es) 2022-06-29 2023-12-29 Pairwise Plants Services Inc Métodos y composiciones para controlar el tamaño del meristemo para el mejoramiento de cultivos
EP4299733A1 (en) 2022-06-30 2024-01-03 Inari Agriculture Technology, Inc. Compositions, systems, and methods for genome editing
EP4299739A1 (en) 2022-06-30 2024-01-03 Inari Agriculture Technology, Inc. Compositions, systems, and methods for genome editing
WO2024005863A1 (en) 2022-06-30 2024-01-04 Inari Agriculture Technology, Inc. Compositions, systems, and methods for genome editing
WO2024005864A1 (en) 2022-06-30 2024-01-04 Inari Agriculture Technology, Inc. Compositions, systems, and methods for genome editing
WO2024020587A2 (en) 2022-07-22 2024-01-25 Tome Biosciences, Inc. Pleiopluripotent stem cell programmable gene insertion
AU2023314808A1 (en) 2022-07-29 2025-03-20 Regeneron Pharmaceuticals, Inc. Compositions and methods for transferrin receptor (tfr)-mediated delivery to the brain and muscle
CN119768514A (zh) 2022-08-04 2025-04-04 成对植物服务股份有限公司 用于改善产量性状的方法和组合物
UY40388A (es) 2022-08-11 2024-03-15 Pairwise Plants Services Inc Métodos y composiciones para controlar el tamaño del meristema para la mejora de los cultivos
IL318914A (en) 2022-08-12 2025-04-01 Life Edit Therapeutics Inc RNA-guided nucleases and active fragments and variants thereof and methods of use
WO2024038168A1 (en) 2022-08-19 2024-02-22 UCB Biopharma SRL Novel rna-guided nucleases and nucleic acid targeting systems comprising such
US20240100192A1 (en) 2022-08-25 2024-03-28 Massachusetts Institute Of Technology Programmable rna writing using crispr effectors and trans-splicing templates
CN120322549A (zh) 2022-08-25 2025-07-15 生命编辑治疗股份有限公司 利用锁核酸对向导rna化学修饰以用于rna引导的核酸酶介导的基因编辑
WO2024042165A2 (en) 2022-08-26 2024-02-29 UCB Biopharma SRL Novel rna-guided nucleases and nucleic acid targeting systems comprising such rna-guided nucleases
US20250386788A1 (en) 2022-08-26 2025-12-25 KWS SAAT SE & Co. KGaA Use of paired genes in hybrid breeding
WO2024042168A1 (en) 2022-08-26 2024-02-29 UCB Biopharma SRL Novel rna-guided nucleases and nucleic acid targeting systems comprising such rna-guided nucleases
CA3266561A1 (en) 2022-09-02 2024-03-07 Janssen Biotech, Inc. BIOMATERIALS AND METHODS OF MODULATING THE HYPOIMMUNOGENIC IMMUNE SYNAPSE
WO2024050548A2 (en) * 2022-09-02 2024-03-07 Hunterian Medicine Llc Compact promoters for targeting hypoxia induced genes
EP4583876A1 (en) 2022-09-08 2025-07-16 Juno Therapeutics, Inc. Combination of a t cell therapy and continuous or intermittent dgk inhibitor dosing
UY40418A (es) 2022-09-08 2024-04-02 Pairwise Plants Services Inc Métodos y composiciones para mejorar características de rendimiento en plantas
WO2024062138A1 (en) 2022-09-23 2024-03-28 Mnemo Therapeutics Immune cells comprising a modified suv39h1 gene
WO2024073606A1 (en) 2022-09-28 2024-04-04 Regeneron Pharmaceuticals, Inc. Antibody resistant modified receptors to enhance cell-based therapies
WO2024073751A1 (en) 2022-09-29 2024-04-04 Vor Biopharma Inc. Methods and compositions for gene modification and enrichment
US12091689B2 (en) 2022-09-30 2024-09-17 Vedabio, Inc. Delivery of therapeutics in vivo via a CRISPR-based cascade system
WO2024076473A1 (en) * 2022-10-02 2024-04-11 Vedabio, Inc. Dimerization screening assays
US11965205B1 (en) 2022-10-14 2024-04-23 Vedabio, Inc. Detection of nucleic acid and non-nucleic acid target molecules
WO2024083579A1 (en) 2022-10-20 2024-04-25 Basf Se Regulatory nucleic acid molecules for enhancing gene expression in plants
WO2024098024A1 (en) 2022-11-04 2024-05-10 Iovance Biotherapeutics, Inc. Expansion of tumor infiltrating lymphocytes from liquid tumors and therapeutic uses thereof
JP2025537155A (ja) 2022-11-04 2025-11-14 アイオバンス バイオセラピューティクス，インコーポレイテッド Ｃｄ３９／ｃｄ１０３選択に関連した腫瘍浸潤リンパ球（ｔｉｌ）拡張のための方法
EP4612284A2 (en) 2022-11-04 2025-09-10 Life Edit Therapeutics, Inc. Evolved adenine deaminases and rna-guided nuclease fusion proteins with internal insertion sites and methods of use
EP4612184A1 (en) 2022-11-04 2025-09-10 Regeneron Pharmaceuticals, Inc. Calcium voltage-gated channel auxiliary subunit gamma 1 (cacng1) binding proteins and cacng1-mediated delivery to skeletal muscle
CN120239706A (zh) 2022-11-07 2025-07-01 先正达农作物保护股份公司 改变大豆植物开花时间和/或成熟的基因及其用途
EP4615960A1 (en) 2022-11-09 2025-09-17 C3S2 GmbH Methods for manufacturing engineered immune cells
WO2024102434A1 (en) 2022-11-10 2024-05-16 Senda Biosciences, Inc. Rna compositions comprising lipid nanoparticles or lipid reconstructed natural messenger packs
WO2024107765A2 (en) 2022-11-14 2024-05-23 Regeneron Pharmaceuticals, Inc. Compositions and methods for fibroblast growth factor receptor 3-mediated delivery to astrocytes
KR20250122544A (ko) 2022-11-21 2025-08-13 이오반스 바이오테라퓨틱스, 인크. 종양 침윤 림프구의 확장을 위한 2차원 공정 및 이를 통한 요법
WO2024118836A1 (en) 2022-11-30 2024-06-06 Iovance Biotherapeutics, Inc. Processes for production of tumor infiltrating lymphocytes with shortened rep step
EP4634383A1 (en) 2022-12-16 2025-10-22 Life Edit Therapeutics, Inc. Guide rnas that target foxp3 gene and methods of use
CN120500534A (zh) 2022-12-16 2025-08-15 成对植物服务股份有限公司 包含Cas12a多肽和内含肽的融合蛋白和其使用方法
EP4634384A1 (en) 2022-12-16 2025-10-22 Life Edit Therapeutics, Inc. Guide rnas that target trac gene and methods of use
US20240271109A1 (en) 2022-12-21 2024-08-15 Pairwise Plants Services, Inc. Engineered proteins and methods of use thereof
WO2024138194A1 (en) 2022-12-22 2024-06-27 Tome Biosciences, Inc. Platforms, compositions, and methods for in vivo programmable gene insertion
CN118275664A (zh) * 2022-12-30 2024-07-02 上海吐露港生物科技有限公司 基于拆分Cas蛋白的非核酸靶标检测方法及应用
WO2024253707A2 (en) 2023-01-07 2024-12-12 Vedabio, Inc. Engineered nucleic acid-guided nucleases
WO2024151493A1 (en) 2023-01-10 2024-07-18 Vedabio, Inc. Sample splitting for multiplexed detection of nucleic acids without amplification
CN121002052A (zh) 2023-02-03 2025-11-21 C3S2 有限公司 用于非病毒生产工程化免疫细胞的方法
WO2024167317A1 (ko) * 2023-02-10 2024-08-15 주식회사 엔세이지 신규 CRISPR/Cas12a 조성물 및 이의 표적 핵산 검출 용도
IL322416A (en) 2023-02-15 2025-09-01 Arbor Biotechnologies Inc Gene editing method to inhibit abnormal splicing in stathmin 2 (stmn2) transcription
WO2024173622A1 (en) 2023-02-16 2024-08-22 Pairwise Plants Services, Inc. Methods and compositions for modifying shade avoidance in plants
WO2024175308A1 (en) 2023-02-22 2024-08-29 KWS SAAT SE & Co. KGaA Plants with improved performance
CN116355878B (zh) * 2023-02-28 2024-04-26 华中农业大学 新型TnpB编程性核酸酶及其应用
WO2024182746A1 (en) 2023-03-01 2024-09-06 Pairwise Plants Services, Inc. Engineered proteins and methods of use thereof
UY40661A (es) 2023-03-02 2024-10-15 Pairwise Plants Services Inc Métodos y composiciones para modificar la evitación de la sombra en plantas
UY40664A (es) 2023-03-09 2024-10-15 Pairwise Plants Services Inc Modificación de genes de la vía de señalización de brasinoesteroide para mejorar rasgos de rendimien
EP4680287A1 (en) 2023-03-15 2026-01-21 Renagade Therapeutics Management Inc. Delivery of gene editing systems and methods of use thereof
WO2024201368A1 (en) 2023-03-29 2024-10-03 Astrazeneca Ab Use of inhibitors to increase efficiency of crispr/cas insertions
PE20252680A1 (es) 2023-03-30 2025-11-24 Pairwise Plants Services Inc Metodos y composiciones para reducir las espinas o espinillas en plantas
WO2024211287A1 (en) 2023-04-03 2024-10-10 Seagen Inc. Production cell lines with targeted integration sites
WO2024211306A1 (en) 2023-04-03 2024-10-10 Sutro Biopharma, Inc. Rf1 ko e. coli strains
WO2024216116A1 (en) 2023-04-14 2024-10-17 Precision Biosciences, Inc. Muscle-specific expression cassettes
CN121311497A (zh) 2023-04-14 2026-01-09 精密生物科学公司 肌肉特异性表达盒
WO2024218295A1 (en) 2023-04-21 2024-10-24 Vib Vzw Allelic combinations in crops for yield increase
CN116751763B (zh) * 2023-05-08 2024-02-13 珠海舒桐医疗科技有限公司 一种Cpf1蛋白、V型基因编辑系统及应用
WO2024234006A1 (en) 2023-05-11 2024-11-14 Tome Biosciences, Inc. Systems, compositions, and methods for targeting liver sinusodial endothelial cells (lsecs)
WO2024235991A1 (en) 2023-05-15 2024-11-21 UCB Biopharma SRL Rna-guided nucleases and nucleic acid targeting systems comprising such rna-guided nucleases
WO2024236547A1 (en) 2023-05-18 2024-11-21 Inceptor Bio, Llc Modified phagocytic cells expressing chimeric antigen receptors comprising a herpes virus entry mediator (hvem) co-stimulatory domain and uses thereof
UY40746A (es) 2023-05-18 2024-12-13 Pairwise Plants Services Inc Métodos y composiciones para mejorar las características de rendimiento de las plantas
WO2024243438A2 (en) 2023-05-23 2024-11-28 Omega Therapeutics, Inc. Compositions and methods for reducing cxcl9, cxcl10, and cxcl11 gene expression
WO2025003358A2 (en) 2023-06-29 2025-01-02 UCB Biopharma SRL Novel nucleic acid targeting systems comprising rna-guided nucleases
AU2024309884A1 (en) 2023-06-30 2025-12-18 Regeneron Pharmaceuticals, Inc. Methods and compositions for increasing homology-directed repair
CN116671491B (zh) * 2023-07-04 2024-01-23 四川省畜牧科学研究院 一种南江黄羊的种羊选育方法
WO2025010912A1 (zh) 2023-07-07 2025-01-16 山东舜丰生物科技有限公司 一种工程化的Cas蛋白及其应用
US12291729B2 (en) 2023-07-07 2025-05-06 Shandong Shunfeng Biotechnology Co.Ltd. Engineered Cas protein and use thereof
CN116790559B (zh) * 2023-07-07 2023-11-28 山东舜丰生物科技有限公司 一种融合HNH结构域的V型Cas酶及其应用
PY2455894A (es) 2023-07-18 2025-03-28 Pairwise Plants Services Inc Métodos y composiciones para modificar la arquitectura radicular en plantas
PY2461802A (es) 2023-07-27 2025-05-19 Monsanto Tech Llc Métodos y composiciones para modificar rasgos de rendimiento de las plantas
WO2025022367A2 (en) 2023-07-27 2025-01-30 Life Edit Therapeutics, Inc. Rna-guided nucleases and active fragments and variants thereof and methods of use
AU2024315073A1 (en) 2023-07-28 2026-01-22 Regeneron Pharmaceuticals, Inc. Use of bgh-sv40l tandem polya to enhance transgene expression during unidirectional gene insertion
AR133384A1 (es) 2023-07-28 2025-09-24 Regeneron Pharma Anti-tfr:esfingomielinasa ácida para el tratamiento de la deficiencia de esfingomielinasa ácida
AU2024317483A1 (en) 2023-07-28 2026-01-29 Regeneron Pharmaceuticals, Inc. Anti-tfr:gaa and anti-cd63:gaa insertion for treatment of pompe disease
WO2025027165A1 (en) 2023-08-01 2025-02-06 Basf Plant Science Company Gmbh Increased resistance by expression of an ics protein
AR133439A1 (es) 2023-08-01 2025-10-01 Kws Saat Se & Co Kgaa Método para reducir los sabores desagradables en proteínas alternativas
AU2024319046A1 (en) 2023-08-01 2026-01-29 KWS SAAT SE & Co. KGaA Method of improving gelation properties of alternative proteins
CN121605117A (zh) 2023-08-01 2026-03-03 巴斯夫植物科学有限公司 通过表达msbp1蛋白增加抗性
WO2025049524A1 (en) 2023-08-28 2025-03-06 Regeneron Pharmaceuticals, Inc. Cxcr4 antibody-resistant modified receptors
WO2025046513A1 (en) 2023-08-29 2025-03-06 Inceptor Bio, Llc Methods of manufacturing myeloid-derived cells from hematopoietic stem cells and compositions and uses thereof
WO2025049959A2 (en) 2023-09-01 2025-03-06 Renagade Therapeutics Management Inc. Gene editing systems, compositions, and methods for treatment of vexas syndrome
WO2025050069A1 (en) 2023-09-01 2025-03-06 Tome Biosciences, Inc. Programmable gene insertion using engineered integration enzymes
WO2025054540A1 (en) 2023-09-08 2025-03-13 Iovance Biotherapeutics, Inc. Methods of gene-editing using programmable nucleases
KR102789465B1 (ko) * 2023-09-20 2025-04-04 주식회사 엔세이지 신규 CRISPR/Cas12a 시스템 및 그 유전자 편집 용도
WO2025072430A1 (en) 2023-09-26 2025-04-03 Pairwise Plants Services, Inc. Crispr-cas fusion proteins with adenine base editor functionality, compositions comprising the same, and methods of use thereof
WO2025076141A1 (en) 2023-10-03 2025-04-10 Inari Agriculture Technology, Inc. Viral delivery of grna to the scion
US20250129348A1 (en) 2023-10-09 2025-04-24 Pairwise Plants Services, Inc. Circular permutants, compositions comprising the same, and methods of use thereof
WO2025080600A1 (en) 2023-10-11 2025-04-17 Pairwise Plants Services, Inc. Methods and compositions for improving crop yield traits
WO2025101484A1 (en) 2023-11-06 2025-05-15 Iovance Biotherapeutics, Inc. Treatment of endometrial cancers with tumor infiltrating lymphocyte therapies
EP4560016A1 (en) * 2023-11-22 2025-05-28 Plantik Biosciences Method of plant genome editing
CN117286039B (zh) * 2023-11-27 2024-03-12 中国科学院天津工业生物技术研究所 一种菌丝形态优化的里氏木霉菌株及其应用
WO2025132866A1 (en) 2023-12-21 2025-06-26 KWS SAAT SE & Co. KGaA Maize plants with improved disease resistance
WO2025137408A1 (en) 2023-12-21 2025-06-26 Pairwise Plants Services, Inc. Compositions and methods for rna-encoded dna-replacement of alleles
WO2025153582A1 (en) 2024-01-17 2025-07-24 KWS SAAT SE & Co. KGaA Maize plants with improved disease resistance
WO2025162985A1 (en) 2024-01-30 2025-08-07 Basf Plant Science Company Gmbh Increased plant disease resistance by expression of a glycine-rich protein
WO2025168705A1 (en) 2024-02-08 2025-08-14 Vib Vzw Means and methods for the production of saponins with endosomal escape-enhancing properties
WO2025171210A1 (en) 2024-02-09 2025-08-14 Arbor Biotechnologies, Inc. Compositions and methods for gene editing via homology-mediated end joining
WO2025174765A1 (en) 2024-02-12 2025-08-21 Renagade Therapeutics Management Inc. Lipid nanoparticles comprising coding rna molecules for use in gene editing and as vaccines and therapeutic agents
WO2025174908A1 (en) 2024-02-12 2025-08-21 Life Edit Therapeutics, Inc. Novel rna-guided nucleases and proteins for polymerase editing
WO2025172110A1 (en) 2024-02-16 2025-08-21 Nunhems Netherlands B.V. Modified plant architecture in cucumber
US20250270578A1 (en) 2024-02-22 2025-08-28 Pairwise Plants Services, Inc. Methods and compositions for improving yield characteristics in plants
US12480141B2 (en) 2024-04-04 2025-11-25 Alia Therapeutics Srl Type V Cas proteins and applications thereof
WO2025224107A1 (en) 2024-04-22 2025-10-30 Basecamp Research Ltd Method and compositions for detecting off-target editing
WO2025224182A2 (en) 2024-04-23 2025-10-30 Basecamp Research Ltd Single construct platform for simultaneous delivery of gene editing machinery and nucleic acid cargo
WO2025235388A1 (en) 2024-05-06 2025-11-13 Regeneron Pharmaceuticals, Inc. Transgene genomic identification by nuclease-mediated long read sequencing
WO2025237806A1 (en) 2024-05-13 2025-11-20 Nunhems Netherlands B.V. Lettuce plant having delayed bolting and shade tolerance
WO2025240947A1 (en) 2024-05-17 2025-11-20 University Of Massachusetts Therapeutic treatment for fragile x-associated disorder
US20250388890A1 (en) 2024-06-20 2025-12-25 Regeneron Pharmaceuticals, Inc. ASS1 Gene Insertion For The Treatment Of Citrullinemia Type I
WO2026003754A1 (en) 2024-06-25 2026-01-02 Life Edit Therapeutics, Inc. Novel reverse transcriptases and uses thereof
US12428636B1 (en) 2024-07-08 2025-09-30 Pairwise Plants Services, Inc. Methods and compositions for modification of protospacer adjacent motif specificity of CAS12A
WO2026015427A1 (en) 2024-07-08 2026-01-15 Pairwise Plants Services, Inc. Methods and compositions for modification of protospacer adjacent motif specificity of cas12a
WO2026015829A2 (en) 2024-07-12 2026-01-15 Arbor Biotechnologies, Inc. Small reverse transcriptases and gene editing systems comprising such
WO2026015832A2 (en) 2024-07-12 2026-01-15 Arbor Biotechnologies, Inc. Reverse transcriptases and gene editing systems comprising such
US20260022365A1 (en) 2024-07-17 2026-01-22 Pairwise Plants Services, Inc. Engineered cytidine deaminases
WO2026025092A1 (en) 2024-07-26 2026-01-29 Juno Therapeutics, Inc. Synthetic promoters for t cell expression
FR3165152A3 (fr) 2024-08-01 2026-02-06 KWS SAAT SE & Co. KGaA Lignées de pois au profil gustatif amélioré
WO2026027749A1 (en) 2024-08-01 2026-02-05 KWS SAAT SE & Co. KGaA Pea lines with improved taste profile of a pea ingredient
EP4686414A1 (en) 2024-08-01 2026-02-04 KWS SAAT SE & Co. KGaA Method of improving gelation properties of pea proteins
US20260043035A1 (en) 2024-08-08 2026-02-12 Invaio Sciences, Inc. Nature-derived lipid particles for use in agriculture
WO2026047626A1 (en) 2024-08-30 2026-03-05 Inceptor Bio, Llc Compositions and methods involving immune cells engineered for binding bispecific antibodies for use in cell therapy
CN119979720B (zh) * 2025-02-26 2025-09-23 中国农业科学院北京畜牧兽医研究所 猪精液品质相关的分子标记鉴定及其应用
CN121343008A (zh) * 2025-03-21 2026-01-16 华东理工大学 一种dhodh多肽降解剂及其在制备预防和/或治疗dhodh介导的疾病的药物中的应用

Family Cites Families (158)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1506509A (en) 1975-12-19 1978-04-05 Nissan Motor Apparatus for mounting instruments on the instrument panel of a motor vehicle
US4217344A (en) 1976-06-23 1980-08-12 L'oreal Compositions containing aqueous dispersions of lipid spheres
US4235871A (en) 1978-02-24 1980-11-25 Papahadjopoulos Demetrios P Method of encapsulating biologically active materials in lipid vesicles
US4186183A (en) 1978-03-29 1980-01-29 The United States Of America As Represented By The Secretary Of The Army Liposome carriers in chemotherapy of leishmaniasis
US4261975A (en) 1979-09-19 1981-04-14 Merck & Co., Inc. Viral liposome particle
US4485054A (en) 1982-10-04 1984-11-27 Lipoderm Pharmaceuticals Limited Method of encapsulating biologically active materials in multilamellar lipid vesicles (MLV)
US4501728A (en) 1983-01-06 1985-02-26 Technology Unlimited, Inc. Masking of liposomes from RES recognition
US4897355A (en) 1985-01-07 1990-01-30 Syntex (U.S.A.) Inc. N[ω,(ω-1)-dialkyloxy]- and N-[ω,(ω-1)-dialkenyloxy]-alk-1-yl-N,N,N-tetrasubstituted ammonium lipids and uses therefor
US4946787A (en) 1985-01-07 1990-08-07 Syntex (U.S.A.) Inc. N-(ω,(ω-1)-dialkyloxy)- and N-(ω,(ω-1)-dialkenyloxy)-alk-1-yl-N,N,N-tetrasubstituted ammonium lipids and uses therefor
US5049386A (en) 1985-01-07 1991-09-17 Syntex (U.S.A.) Inc. N-ω,(ω-1)-dialkyloxy)- and N-(ω,(ω-1)-dialkenyloxy)Alk-1-YL-N,N,N-tetrasubstituted ammonium lipids and uses therefor
US4797368A (en) 1985-03-15 1989-01-10 The United States Of America As Represented By The Department Of Health And Human Services Adeno-associated virus as eukaryotic expression vector
US4774085A (en) 1985-07-09 1988-09-27 501 Board of Regents, Univ. of Texas Pharmaceutical administration systems containing a mixture of immunomodulators
DE122007000007I1 (de) 1986-04-09 2007-05-16 Genzyme Corp Genetisch transformierte Tiere, die ein gewünschtes Protein in Milch absondern
US4837028A (en) 1986-12-24 1989-06-06 Liposome Technology, Inc. Liposomes with enhanced circulation time
US4873316A (en) 1987-06-23 1989-10-10 Biogen, Inc. Isolation of exogenous recombinant proteins from the milk of transgenic mammals
US5703055A (en) 1989-03-21 1997-12-30 Wisconsin Alumni Research Foundation Generation of antibodies through lipid mediated DNA delivery
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5264618A (en) 1990-04-19 1993-11-23 Vical, Inc. Cationic lipids for intracellular delivery of biologically active molecules
AU7979491A (en) 1990-05-03 1991-11-27 Vical, Inc. Intracellular delivery of biologically active substances by means of self-assembling lipid complexes
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5266317A (en) 1990-10-04 1993-11-30 University Of Georgia Research Foundation, Inc. Insect-specific paralytic neurotoxin genes for use in biological insect control: methods and compositions
US5173414A (en) 1990-10-30 1992-12-22 Applied Immune Sciences, Inc. Production of recombinant adeno-associated virus vectors
GB9104617D0 (en) 1991-03-05 1991-04-17 Nickerson Int Seed Pest control
GB9114259D0 (en) 1991-07-02 1991-08-21 Ici Plc Plant derived enzyme and dna sequences
GB9115909D0 (en) 1991-07-23 1991-09-04 Nickerson Int Seed Recombinant dna
DE4124514A1 (de) 1991-07-24 1993-01-28 Bosch Gmbh Robert Beschleunigungssensor, insbesondere zum selbsttaetigen ausloesen von insassenschutzvorrichtungen in kraftfahrzeugen
DK39692D0 (da) 1992-03-25 1992-03-25 Danisco Biologisk materiale
US5587308A (en) 1992-06-02 1996-12-24 The United States Of America As Represented By The Department Of Health & Human Services Modified adeno-associated virus vector capable of expression from a novel promoter
BR9306802A (pt) 1992-07-27 1998-12-08 Pioneer Hi Bred Int Processo independente de genótipos para produção de planta de soja transgénica e processo de regeneração de plantas de soja a partir de nodos cotiledonais
US5593972A (en) 1993-01-26 1997-01-14 The Wistar Institute Genetic immunization
US5789156A (en) 1993-06-14 1998-08-04 Basf Ag Tetracycline-regulated transcriptional inhibitors
US5814618A (en) 1993-06-14 1998-09-29 Basf Aktiengesellschaft Methods for regulating gene expression
US5543158A (en) 1993-07-23 1996-08-06 Massachusetts Institute Of Technology Biodegradable injectable nanoparticles
US6007845A (en) 1994-07-22 1999-12-28 Massachusetts Institute Of Technology Nanoparticles and microparticles of non-linear hydrophilic-hydrophobic multiblock copolymers
US5994627A (en) 1995-03-31 1999-11-30 Common Wealth Scientific And Industrial Research Organisation Genetic sequences conferring nematode resistance in plants and uses therefor
EP0831854A4 (en) 1995-06-06 2001-01-24 Isis Pharmaceuticals Inc OLIGONUCLEOTIDS WITH PHOSPHOROTHIOATE BINDINGS OF HIGH CHIRAL PURITY
US5985662A (en) 1995-07-13 1999-11-16 Isis Pharmaceuticals Inc. Antisense inhibition of hepatitis B virus replication
AR006928A1 (es) 1996-05-01 1999-09-29 Pioneer Hi Bred Int Una molecula de adn aislada que codifica una proteina fluorescente verde como marcador rastreable para la transformacion de plantas, un metodo para laproduccion de plantas transgenicas, un vector de expresion, una planta transgenica y celulas de dichas plantas.
US5985309A (en) 1996-05-24 1999-11-16 Massachusetts Institute Of Technology Preparation of particles for inhalation
US5855913A (en) 1997-01-16 1999-01-05 Massachusetts Instite Of Technology Particles incorporating surfactants for pulmonary drug delivery
US5846946A (en) 1996-06-14 1998-12-08 Pasteur Merieux Serums Et Vaccins Compositions and methods for administering Borrelia DNA
GB9907461D0 (en) 1999-03-31 1999-05-26 King S College London Neurite regeneration
US7022896B1 (en) 1997-04-04 2006-04-04 Board Of Regents Of University Of Nebraska Methods and materials for making and using transgenic dicamba-degrading organisms
GB9720465D0 (en) 1997-09-25 1997-11-26 Oxford Biomedica Ltd Dual-virus vectors
AU752704B2 (en) 1997-10-24 2002-09-26 Invitrogen Corporation Recombinational cloning using nucleic acids having recombination sites
US6750059B1 (en) 1998-07-16 2004-06-15 Whatman, Inc. Archiving of vectors
US6534261B1 (en) 1999-01-12 2003-03-18 Sangamo Biosciences, Inc. Regulation of endogenous gene expression in cells using zinc finger proteins
US7868149B2 (en) 1999-07-20 2011-01-11 Monsanto Technology Llc Plant genome sequence and uses thereof
US6603061B1 (en) 1999-07-29 2003-08-05 Monsanto Company Agrobacterium-mediated plant transformation method
GB0024550D0 (es) 2000-10-06 2000-11-22 Oxford Biomedica Ltd
AU2002336760A1 (en) 2001-09-26 2003-06-10 Mayo Foundation For Medical Education And Research Mutable vaccines
GB0125216D0 (en) 2001-10-19 2001-12-12 Univ Strathclyde Dendrimers for use in targeted delivery
US20090100536A1 (en) 2001-12-04 2009-04-16 Monsanto Company Transgenic plants with enhanced agronomic traits
JP2005512598A (ja) 2001-12-21 2005-05-12 オックスフォード バイオメディカ （ユーケー） リミテッド Ｅｉａｖなどのレンチウイルス発現ベクターを使用するトランスジェニック生物の作製方法
DE60334618D1 (de) 2002-06-28 2010-12-02 Protiva Biotherapeutics Inc Verfahren und vorrichtung zur herstellung von liposomen
GB0220467D0 (en) 2002-09-03 2002-10-09 Oxford Biomedica Ltd Composition
EP1558724A4 (en) 2002-11-01 2006-08-02 New England Biolabs Inc ORGANIC RNA SCREENING AND ITS USE IN INTERRUPTING GENE TRANSMISSION IN THE ENVIRONMENT
CN101291653B (zh) 2003-07-16 2012-06-27 普洛体维生物治疗公司 脂质包封的干扰rna
KR101164256B1 (ko) 2003-09-15 2012-07-10 프로티바 바이오쎄라퓨틱스, 인코포레이티드 폴리에틸렌글리콜 개질된 지질 화합물 및 그의 용도
GB0325379D0 (en) 2003-10-30 2003-12-03 Oxford Biomedica Ltd Vectors
US7919277B2 (en) * 2004-04-28 2011-04-05 Danisco A/S Detection and typing of bacterial strains
EP1781593B1 (en) 2004-06-07 2011-12-14 Protiva Biotherapeutics Inc. Cationic lipids and methods of use
EP1766035B1 (en) 2004-06-07 2011-12-07 Protiva Biotherapeutics Inc. Lipid encapsulated interfering rna
EP1784416B1 (en) 2004-07-16 2011-10-05 GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES Vaccines against aids comprising cmv/r nucleic acid constructs
GB0422877D0 (en) 2004-10-14 2004-11-17 Univ Glasgow Bioactive polymers
US7838658B2 (en) 2005-10-20 2010-11-23 Ian Maclachlan siRNA silencing of filovirus gene expression
JP5043672B2 (ja) 2005-10-28 2012-10-10 田辺三菱製薬株式会社 新規細胞膜透過ペプチド
CN101346393B (zh) 2005-11-02 2015-07-22 普洛体维生物治疗公司 修饰的siRNA分子及其应用
GB0526211D0 (en) 2005-12-22 2006-02-01 Oxford Biomedica Ltd Viral vectors
US7915399B2 (en) 2006-06-09 2011-03-29 Protiva Biotherapeutics, Inc. Modified siRNA molecules and uses thereof
JP2008078613A (ja) 2006-08-24 2008-04-03 Rohm Co Ltd 窒化物半導体の製造方法及び窒化物半導体素子
US20100099858A1 (en) 2006-09-28 2010-04-22 Mirkin Chad A Maximizing Oligonucleotide Loading on Gold Nanoparticle
EP2121977B1 (en) 2007-01-11 2017-06-21 Erasmus University Medical Center Circular chromosome conformation capture (4c)
US7838729B2 (en) 2007-02-26 2010-11-23 Monsanto Technology Llc Chloroplast transit peptides for efficient targeting of DMO and uses thereof
WO2008149176A1 (en) 2007-06-06 2008-12-11 Cellectis Meganuclease variants cleaving a dna target sequence from the mouse rosa26 locus and uses thereof
JP5588344B2 (ja) 2007-08-14 2014-09-10 フレッド ハッチンソン キャンサー リサーチ センター 治療薬をデリバリーするための針アレイアセンブリ及び方法
CA2910760C (en) 2007-12-04 2019-07-09 Muthiah Manoharan Targeting lipids
US8404658B2 (en) 2007-12-31 2013-03-26 Nanocor Therapeutics, Inc. RNA interference for the treatment of heart failure
CA2721333C (en) 2008-04-15 2020-12-01 Protiva Biotherapeutics, Inc. Novel lipid formulations for nucleic acid delivery
FR2930264B1 (fr) * 2008-04-18 2013-02-22 Gervais Danone Sa Nouvelle souche de lactobacillus paracasei subsp. paracasei dotee de proprietes antimicrobiennes et immunomodulatrices.
US8575305B2 (en) 2008-06-04 2013-11-05 Medical Research Council Cell penetrating peptides
CN102137658A (zh) 2008-06-30 2011-07-27 斯兰斯德有限公司 局部递送药物的方法、组合物和系统
WO2010004594A1 (en) 2008-07-08 2010-01-14 S.I.F.I. Societa' Industria Farmaceutica Italiana S.P.A. Ophthalmic compositions for treating pathologies of the posterior segment of the eye
US20100076057A1 (en) 2008-09-23 2010-03-25 Northwestern University TARGET DNA INTERFERENCE WITH crRNA
WO2010053572A2 (en) 2008-11-07 2010-05-14 Massachusetts Institute Of Technology Aminoalcohol lipidoids and uses thereof
GB2465749B (en) 2008-11-25 2013-05-08 Algentech Sas Plant cell transformation method
WO2010129602A2 (en) 2009-05-04 2010-11-11 Fred Hutchinson Cancer Research Center Cocal vesiculovirus envelope pseudotyped retroviral vectors
JP5766188B2 (ja) 2009-07-01 2015-08-19 プロチバ バイオセラピューティクス インコーポレイティッド 固形腫瘍に治療剤を送達するための脂質製剤
WO2011000108A1 (en) 2009-07-01 2011-01-06 Protiva Biotherapeutics, Inc. Compositions and methods for silencing apolipoprotein b
US8927807B2 (en) 2009-09-03 2015-01-06 The Regents Of The University Of California Nitrate-responsive promoter
US8889394B2 (en) 2009-09-07 2014-11-18 Empire Technology Development Llc Multiple domain proteins
WO2011076807A2 (en) 2009-12-23 2011-06-30 Novartis Ag Lipids, lipid compositions, and methods of using them
SG183374A1 (en) 2010-02-24 2012-09-27 Arrowhead Res Corp Compositions for targeted delivery of sirna
KR101880536B1 (ko) 2010-04-26 2018-07-23 상가모 테라퓨틱스, 인코포레이티드 아연 핑거 뉴클레아제를 사용하는 로사 좌위의 게놈 편집
US8372951B2 (en) 2010-05-14 2013-02-12 National Tsing Hua University Cell penetrating peptides for intracellular delivery
EP2575894B1 (en) 2010-05-28 2015-02-25 Oxford Biomedica (UK) Ltd Delivery of lentiviral vectors to the brain
US9193827B2 (en) 2010-08-26 2015-11-24 Massachusetts Institute Of Technology Poly(beta-amino alcohols), their preparation, and uses thereof
BR112013009583A2 (pt) * 2010-10-20 2017-05-30 Dupont Nutrition Biosci Aps ácido nucleico, vetor, célula hospedeira, métodos de preparação de uma linhagem bacteriana variante de tipificação, de marcação, de geração e de controle de populações bacterianas, linhagem e célula bacteriana variante, kit, uso de um ácido nucleico, cultivo celular, produto, processo de preparação de produtos, mutantes de fago e mutante de escape de fago
US9405700B2 (en) 2010-11-04 2016-08-02 Sonics, Inc. Methods and apparatus for virtualization in an integrated circuit
US9238716B2 (en) 2011-03-28 2016-01-19 Massachusetts Institute Of Technology Conjugated lipomers and uses thereof
JP2014511687A (ja) 2011-03-31 2014-05-19 モデルナ セラピューティクス インコーポレイテッド 工学操作された核酸の送達および製剤
US20120295960A1 (en) 2011-05-20 2012-11-22 Oxford Biomedica (Uk) Ltd. Treatment regimen for parkinson's disease
CA2849996C (en) 2011-09-26 2018-04-03 Sumitomo Chemical Company, Limited Compositions for controlling plant fungal diseases comprising a carboxamide compound and ethaboxam
KR20140102759A (ko) 2011-12-16 2014-08-22 모더나 세라퓨틱스, 인코포레이티드 변형된 뉴클레오사이드, 뉴클레오타이드 및 핵산 조성물
US20130185823A1 (en) 2012-01-16 2013-07-18 Academia Sinica Mesoporous silica nanoparticle-mediated delivery of dna into arabidopsis root
US9637739B2 (en) * 2012-03-20 2017-05-02 Vilnius University RNA-directed DNA cleavage by the Cas9-crRNA complex
WO2013158141A1 (en) 2012-04-18 2013-10-24 Arrowhead Research Corporation Poly(acrylate) polymers for in vivo nucleic acid delivery
PL4289948T3 (pl) 2012-05-25 2025-06-02 The Regents Of The University Of California Sposoby i kompozycje do modyfikacji kierowanego na rna docelowego dna i do nakierowanej na rna modulacji transkrypcji
US8614194B1 (en) 2012-07-25 2013-12-24 Kaohsiung Medical University Anionic cell penetrating peptide and its use for intracellular delivery
KR20230065381A (ko) 2012-07-25 2023-05-11 더 브로드 인스티튜트, 인코퍼레이티드 유도 ｄｎａ 결합 단백질 및 게놈 교란 도구 및 이의 적용
UA119135C2 (uk) * 2012-09-07 2019-05-10 ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі Спосіб отримання трансгенної рослини
EP2912175B1 (en) * 2012-10-23 2018-08-22 Toolgen Incorporated Composition for cleaving a target dna comprising a guide rna specific for the target dna and cas protein-encoding nucleic acid or cas protein, and use thereof
CN105121648B (zh) 2012-12-12 2021-05-07 布罗德研究所有限公司 用于序列操纵的系统、方法和优化的指导组合物的工程化
CN105658796B (zh) 2012-12-12 2021-10-26 布罗德研究所有限公司 用于序列操纵的crispr-cas组分系统、方法以及组合物
WO2014093622A2 (en) 2012-12-12 2014-06-19 The Broad Institute, Inc. Delivery, engineering and optimization of systems, methods and compositions for sequence manipulation and therapeutic applications
PT2898075E (pt) 2012-12-12 2016-06-16 Harvard College Manipulação e otimização de sistemas, métodos e composições de enzima melhorados para manipulação de sequências
WO2014093701A1 (en) 2012-12-12 2014-06-19 The Broad Institute, Inc. Functional genomics using crispr-cas systems, compositions, methods, knock out libraries and applications thereof
WO2014093694A1 (en) * 2012-12-12 2014-06-19 The Broad Institute, Inc. Crispr-cas nickase systems, methods and compositions for sequence manipulation in eukaryotes
US8697359B1 (en) * 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products
EP4282970A3 (en) 2012-12-17 2024-01-17 President and Fellows of Harvard College Rna-guided human genome engineering
WO2014118272A1 (en) 2013-01-30 2014-08-07 Santaris Pharma A/S Antimir-122 oligonucleotide carbohydrate conjugates
KR20170108172A (ko) 2013-03-14 2017-09-26 카리부 바이오사이언시스 인코포레이티드 핵산-표적화 핵산의 조성물 및 방법
US20140273230A1 (en) * 2013-03-15 2014-09-18 Sigma-Aldrich Co., Llc Crispr-based genome modification and regulation
CA2913869C (en) 2013-05-29 2023-01-24 Cellectis New compact scaffold of cas9 in the type ii crispr system
MY197877A (en) * 2013-06-04 2023-07-22 Harvard College Rna-guided transcriptional regulation
US20140356956A1 (en) * 2013-06-04 2014-12-04 President And Fellows Of Harvard College RNA-Guided Transcriptional Regulation
KR102307280B1 (ko) * 2013-06-05 2021-10-01 듀크 유니버시티 Rna-가이드 유전자 편집 및 유전자 조절
EP3597755A1 (en) * 2013-06-17 2020-01-22 The Broad Institute, Inc. Delivery, use and therapeutic applications of the crispr-cas systems and compositions for targeting disorders and diseases using viral components
EP3674411A1 (en) 2013-06-17 2020-07-01 The Broad Institute, Inc. Delivery, engineering and optimization of tandem guide systems, methods and compositions for sequence manipulation
CA2915837A1 (en) 2013-06-17 2014-12-24 The Broad Institute, Inc. Optimized crispr-cas double nickase systems, methods and compositions for sequence manipulation
US11306328B2 (en) 2013-07-26 2022-04-19 President And Fellows Of Harvard College Genome engineering
US9340800B2 (en) 2013-09-06 2016-05-17 President And Fellows Of Harvard College Extended DNA-sensing GRNAS
EP3418379B1 (en) 2013-09-18 2020-12-09 Kymab Limited Methods, cells & organisms
JP2016536021A (ja) 2013-11-07 2016-11-24 エディタス・メディシン，インコーポレイテッド ＣＲＩＳＰＲ関連方法および支配ｇＲＮＡのある組成物
WO2015071474A2 (en) 2013-11-18 2015-05-21 Crispr Therapeutics Ag Crispr-cas system materials and methods
WO2015089364A1 (en) 2013-12-12 2015-06-18 The Broad Institute Inc. Crystal structure of a crispr-cas system, and uses thereof
WO2015089354A1 (en) 2013-12-12 2015-06-18 The Broad Institute Inc. Compositions and methods of use of crispr-cas systems in nucleotide repeat disorders
JP2017501149A (ja) 2013-12-12 2017-01-12 ザ・ブロード・インスティテュート・インコーポレイテッド 粒子送達構成成分を用いた障害及び疾患の標的化のためのｃｒｉｓｐｒ−ｃａｓ系及び組成物の送達、使用及び治療適用
EP3080259B1 (en) 2013-12-12 2023-02-01 The Broad Institute, Inc. Engineering of systems, methods and optimized guide compositions with new architectures for sequence manipulation
EP3080271B1 (en) 2013-12-12 2020-02-12 The Broad Institute, Inc. Systems, methods and compositions for sequence manipulation with optimized functional crispr-cas systems
WO2015086795A1 (en) 2013-12-13 2015-06-18 Cellectis Cas9 nuclease platform for microalgae genome engineering
WO2015109752A1 (en) 2014-01-21 2015-07-30 The Institute Of Genetics And Developmental Biology Chinese Academy Of Sciences Modified plants
JP5937635B2 (ja) 2014-03-28 2016-06-22 ファナック株式会社 電磁接触器の溶着検出機能を有するモータ駆動装置
CN104017821B (zh) * 2014-05-16 2016-07-06 安徽省农业科学院水稻研究所 定向编辑颖壳颜色决定基因OsCHI创制褐壳水稻材料的方法
WO2016049258A2 (en) 2014-09-25 2016-03-31 The Broad Institute Inc. Functional screening with optimized functional crispr-cas systems
EP3230452B1 (en) 2014-12-12 2025-06-11 The Broad Institute, Inc. Dead guides for crispr transcription factors
SG11201704272YA (en) * 2014-12-31 2017-06-29 Synthetic Genomics Inc Compositions and methods for high efficiency in vivo genome editing
LT3250691T (lt) * 2015-01-28 2023-09-11 Caribou Biosciences, Inc. Crispr hibridiniai dnr/rnr polinukleotidai ir naudojimo būdai
GB201506509D0 (en) 2015-04-16 2015-06-03 Univ Wageningen Nuclease-mediated genome editing
US20160362667A1 (en) * 2015-06-10 2016-12-15 Caribou Biosciences, Inc. CRISPR-Cas Compositions and Methods
WO2016205749A1 (en) 2015-06-18 2016-12-22 The Broad Institute Inc. Novel crispr enzymes and systems
EP4159856A1 (en) 2015-06-18 2023-04-05 The Broad Institute, Inc. Novel crispr enzymes and systems
US9790490B2 (en) * 2015-06-18 2017-10-17 The Broad Institute Inc. CRISPR enzymes and systems
US20190048340A1 (en) 2015-09-24 2019-02-14 Crispr Therapeutics Ag Novel family of rna-programmable endonucleases and their uses in genome editing and other applications
US12110490B2 (en) 2015-12-18 2024-10-08 The Broad Institute, Inc. CRISPR enzymes and systems
CN105602993A (zh) * 2016-01-19 2016-05-25 上海赛墨生物技术有限公司 线粒体靶向的基因编辑系统及方法
WO2017189308A1 (en) 2016-04-19 2017-11-02 The Broad Institute Inc. Novel crispr enzymes and systems
WO2021133977A1 (en) 2019-12-23 2021-07-01 The Broad Institute, Inc. Programmable dna nuclease-associated ligase and methods of use thereof

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