DK3502253T3 - Hidtil ukendte crispr-enzymer og -systemer - Google Patents

Hidtil ukendte crispr-enzymer og -systemer Download PDF

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Publication number
DK3502253T3
DK3502253T3 DK18188126.9T DK18188126T DK3502253T3 DK 3502253 T3 DK3502253 T3 DK 3502253T3 DK 18188126 T DK18188126 T DK 18188126T DK 3502253 T3 DK3502253 T3 DK 3502253T3
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Denmark
Prior art keywords
systems
still unknown
crispr enzymes
unknown crispr
enzymes
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DK18188126.9T
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English (en)
Inventor
Feng Zhang
Bernd Zetsche
Jonathan S Gootenberg
Omar O Abudayyeh
Ian Slaymaker
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Broad Inst Inc
Massachusetts Inst Technology
Harvard College
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=55274964&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DK3502253(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Broad Inst Inc, Massachusetts Inst Technology, Harvard College filed Critical Broad Inst Inc
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Publication of DK3502253T3 publication Critical patent/DK3502253T3/da

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    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
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    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
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    • A61P21/00—Drugs for disorders of the muscular or neuromuscular system
    • A61P21/04—Drugs for disorders of the muscular or neuromuscular system for myasthenia gravis
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    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2310/16—Aptamers
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
DK18188126.9T 2015-06-18 2016-06-17 Hidtil ukendte crispr-enzymer og -systemer DK3502253T3 (da)

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
US201562181739P 2015-06-18 2015-06-18
US201562193507P 2015-07-16 2015-07-16
US201562201542P 2015-08-05 2015-08-05
US201562205733P 2015-08-16 2015-08-16
US201562232067P 2015-09-24 2015-09-24
US14/975,085 US9790490B2 (en) 2015-06-18 2015-12-18 CRISPR enzymes and systems
EP16150428.7A EP3009511B2 (en) 2015-06-18 2016-01-07 Novel crispr enzymes and systems
EP16734803.6A EP3310917B1 (en) 2015-06-18 2016-06-17 Novel crispr enzymes and systems

Publications (1)

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DK3502253T3 true DK3502253T3 (da) 2020-08-17

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DK16150428.7T DK3009511T3 (da) 2015-06-18 2016-01-07 Hidtil ukendte crispr-enzymer og systemer
DK18188126.9T DK3502253T3 (da) 2015-06-18 2016-06-17 Hidtil ukendte crispr-enzymer og -systemer
DK18186003.2T DK3470519T3 (da) 2015-06-18 2016-06-17 Hidtil ukendte crispr-enzymer og -systemer
DK16734803.6T DK3310917T3 (da) 2015-06-18 2016-06-17 Hidtil ukendte crispr-enzymer og -systemer

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DK16150428.7T DK3009511T3 (da) 2015-06-18 2016-01-07 Hidtil ukendte crispr-enzymer og systemer

Family Applications After (2)

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DK18186003.2T DK3470519T3 (da) 2015-06-18 2016-06-17 Hidtil ukendte crispr-enzymer og -systemer
DK16734803.6T DK3310917T3 (da) 2015-06-18 2016-06-17 Hidtil ukendte crispr-enzymer og -systemer

Country Status (16)

Country Link
US (2) US9790490B2 (da)
EP (5) EP3009511B2 (da)
JP (3) JP6898865B2 (da)
KR (2) KR102613296B1 (da)
CN (6) CN115572737A (da)
AU (3) AU2016278990B2 (da)
CA (1) CA2989834A1 (da)
DK (4) DK3009511T3 (da)
ES (3) ES2646140T3 (da)
HK (2) HK1220726A1 (da)
IL (3) IL305044A (da)
MX (2) MX2017016688A (da)
RU (1) RU2737537C2 (da)
SG (1) SG10201912330QA (da)
TW (1) TWI758251B (da)
WO (1) WO2016205711A1 (da)

Families Citing this family (654)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013066438A2 (en) 2011-07-22 2013-05-10 President And Fellows Of Harvard College Evaluation and improvement of nuclease cleavage specificity
US10704021B2 (en) 2012-03-15 2020-07-07 Flodesign Sonics, Inc. Acoustic perfusion devices
US9458450B2 (en) 2012-03-15 2016-10-04 Flodesign Sonics, Inc. Acoustophoretic separation technology using multi-dimensional standing waves
EP2841572B1 (en) 2012-04-27 2019-06-19 Duke University Genetic correction of mutated genes
BR112015026197B1 (pt) 2013-04-16 2022-12-06 Regeneron Pharmaceuticals, Inc Método para modificação marcada de um lócus genômico de interesse em uma célula de rato pluripotente
EP3011034B1 (en) * 2013-06-17 2019-08-07 The Broad Institute, Inc. Delivery, use and therapeutic applications of the crispr-cas systems and compositions for targeting disorders and diseases using viral components
US9163284B2 (en) 2013-08-09 2015-10-20 President And Fellows Of Harvard College Methods for identifying a target site of a Cas9 nuclease
US9359599B2 (en) 2013-08-22 2016-06-07 President And Fellows Of Harvard College Engineered transcription activator-like effector (TALE) domains and uses thereof
US9526784B2 (en) 2013-09-06 2016-12-27 President And Fellows Of Harvard College Delivery system for functional nucleases
US9322037B2 (en) 2013-09-06 2016-04-26 President And Fellows Of Harvard College Cas9-FokI fusion proteins and uses thereof
US9228207B2 (en) 2013-09-06 2016-01-05 President And Fellows Of Harvard College Switchable gRNAs comprising aptamers
LT3066201T (lt) 2013-11-07 2018-08-10 Editas Medicine, Inc. Su crispr susiję būdai ir kompozicijos su valdančiomis grnr
RU2685914C1 (ru) 2013-12-11 2019-04-23 Регенерон Фармасьютикалс, Инк. Способы и композиции для направленной модификации генома
US20150165054A1 (en) 2013-12-12 2015-06-18 President And Fellows Of Harvard College Methods for correcting caspase-9 point mutations
EP3092049A1 (en) 2014-01-08 2016-11-16 Flodesign Sonics Inc. Acoustophoresis device with dual acoustophoretic chamber
CN106164271B (zh) 2014-02-11 2020-06-02 科罗拉多州立大学董事会（法人团体） Crispr支持的多路基因组工程化
US11491480B2 (en) 2014-06-13 2022-11-08 Children's Medical Center Corporation Products and methods to isolate mitochondria
DK3161128T3 (da) 2014-06-26 2018-11-05 Regeneron Pharma Fremgangsmåder og sammensætninger til målrettede gentiske modifikationer og fremgangsmåder til anvendelse heraf
US10077453B2 (en) 2014-07-30 2018-09-18 President And Fellows Of Harvard College CAS9 proteins including ligand-dependent inteins
ES2741387T3 (es) 2014-10-15 2020-02-10 Regeneron Pharma Métodos y composiciones para generar o mantener células pluripotentes
CA2963820A1 (en) 2014-11-07 2016-05-12 Editas Medicine, Inc. Methods for improving crispr/cas-mediated genome-editing
LT3221457T (lt) 2014-11-21 2019-06-10 Regeneron Pharmaceuticals, Inc. Nukreipiančios genetinės modifikacijos būdai ir kompozicijos, naudojant suporuotas kreipiančiąsias rnr sekas
KR102656470B1 (ko) 2014-12-10 2024-04-09 리전츠 오브 더 유니버스티 오브 미네소타 질환을 치료하기 위한 유전적으로 변형된 세포, 조직 및 장기
NZ732895A (en) 2014-12-19 2022-05-27 Regeneron Pharma Methods and compositions for targeted genetic modification through single-step multiple targeting
EP3277805A1 (en) * 2015-03-31 2018-02-07 Exeligen Scientific, Inc. Cas 9 retroviral integrase and cas 9 recombinase systems for targeted incorporation of a dna sequence into a genome of a cell or organism
GB201506509D0 (en) * 2015-04-16 2015-06-03 Univ Wageningen Nuclease-mediated genome editing
US11377651B2 (en) 2016-10-19 2022-07-05 Flodesign Sonics, Inc. Cell therapy processes utilizing acoustophoresis
US11708572B2 (en) 2015-04-29 2023-07-25 Flodesign Sonics, Inc. Acoustic cell separation techniques and processes
JP7030522B2 (ja) 2015-05-11 2022-03-07 エディタス・メディシン、インコーポレイテッド 幹細胞における遺伝子編集のための最適化ｃｒｉｓｐｒ／ｃａｓ９システムおよび方法
CN107614680A (zh) * 2015-05-14 2018-01-19 南加利福尼亚大学 利用重组核酸内切酶系统的最佳化基因编辑
WO2016201047A1 (en) 2015-06-09 2016-12-15 Editas Medicine, Inc. Crispr/cas-related methods and compositions for improving transplantation
US10648020B2 (en) 2015-06-18 2020-05-12 The Broad Institute, Inc. CRISPR enzymes and systems
US9790490B2 (en) * 2015-06-18 2017-10-17 The Broad Institute Inc. CRISPR enzymes and systems
CA3012631A1 (en) 2015-06-18 2016-12-22 The Broad Institute Inc. Novel crispr enzymes and systems
WO2016205749A1 (en) * 2015-06-18 2016-12-22 The Broad Institute Inc. Novel crispr enzymes and systems
EP3313989A4 (en) 2015-06-29 2018-12-05 Ionis Pharmaceuticals, Inc. Modified crispr rna and modified single crispr rna and uses thereof
WO2017015015A1 (en) * 2015-07-17 2017-01-26 Emory University Crispr-associated protein from francisella and uses related thereto
US11474085B2 (en) 2015-07-28 2022-10-18 Flodesign Sonics, Inc. Expanded bed affinity selection
US11459540B2 (en) 2015-07-28 2022-10-04 Flodesign Sonics, Inc. Expanded bed affinity selection
IL295616A (en) 2015-07-31 2022-10-01 Us Health Adapted cells and treatment methods
US11214800B2 (en) 2015-08-18 2022-01-04 The Broad Institute, Inc. Methods and compositions for altering function and structure of chromatin loops and/or domains
US11530424B1 (en) * 2015-09-18 2022-12-20 University Of South Florida Efficient CRISPR/HDR-mediated knock-in system and method of use
EP3786294A1 (en) 2015-09-24 2021-03-03 Editas Medicine, Inc. Use of exonucleases to improve crispr/cas-mediated genome editing
CA2998287A1 (en) * 2015-09-24 2017-04-20 Crispr Therapeutics Ag Novel family of rna-programmable endonucleases and their uses in genome editing and other applications
EP3940070A1 (en) 2015-10-05 2022-01-19 Precision Biosciences, Inc. Engineered meganucleases with recognition sequences found in the human t cell receptor alpha constant region gene
EP4108255A1 (en) 2015-10-05 2022-12-28 Precision Biosciences, Inc. Genetically-modified cells comprising a modified human t cell receptor alpha constant region gene
IL297017A (en) 2015-10-08 2022-12-01 Harvard College Multiplexed genome editing
CN108513579B (zh) * 2015-10-09 2022-10-04 孟山都技术公司 新颖的rna导向性核酸酶及其用途
US11970710B2 (en) 2015-10-13 2024-04-30 Duke University Genome engineering with Type I CRISPR systems in eukaryotic cells
CA3001130A1 (en) * 2015-10-16 2017-04-20 Temple University - Of The Commonwealth System Of Higher Education Methods and compositions utilizing cpf1 for rna-guided gene editing
ES2699848T3 (es) 2015-10-23 2019-02-13 Caribou Biosciences Inc Acido nucleico CRISPR clase 2 de tipo cruzado modificado que se dirige a ácidos nucleicos
EP3365357B1 (en) 2015-10-23 2024-02-14 President and Fellows of Harvard College Evolved cas9 proteins for gene editing
US20180230489A1 (en) 2015-10-28 2018-08-16 Voyager Therapeutics, Inc. Regulatable expression using adeno-associated virus (aav)
EP3370513A1 (en) * 2015-11-06 2018-09-12 The Jackson Laboratory Large genomic dna knock-in and uses thereof
CN106893739A (zh) * 2015-11-17 2017-06-27 香港中文大学 用于靶向基因操作的新方法和系统
US20170151287A1 (en) 2015-11-30 2017-06-01 Flagship Ventures Management, Inc. Methods and compositions of chondrisomes
US11208649B2 (en) 2015-12-07 2021-12-28 Zymergen Inc. HTP genomic engineering platform
US9988624B2 (en) 2015-12-07 2018-06-05 Zymergen Inc. Microbial strain improvement by a HTP genomic engineering platform
US11293029B2 (en) 2015-12-07 2022-04-05 Zymergen Inc. Promoters from Corynebacterium glutamicum
WO2017099494A1 (ko) * 2015-12-08 2017-06-15 기초과학연구원 Cpf1을 포함하는 유전체 교정용 조성물 및 그 용도
US20190233814A1 (en) * 2015-12-18 2019-08-01 The Broad Institute, Inc. Novel crispr enzymes and systems
EP4159848A1 (en) * 2015-12-29 2023-04-05 Monsanto Technology LLC Novel crispr-associated transposases and uses thereof
US11427837B2 (en) * 2016-01-12 2022-08-30 The Regents Of The University Of California Compositions and methods for enhanced genome editing
US20190264186A1 (en) * 2016-01-22 2019-08-29 The Broad Institute Inc. Crystal structure of crispr cpf1
US9896696B2 (en) 2016-02-15 2018-02-20 Benson Hill Biosystems, Inc. Compositions and methods for modifying genomes
US11447768B2 (en) * 2016-03-01 2022-09-20 University Of Florida Research Foundation, Incorporated Molecular cell diary system
AU2017226172B9 (en) * 2016-03-04 2023-08-24 Editas Medicine, Inc. CRISPR/Cpf1-related methods, compositions and components for cancer immunotherapy
US11597924B2 (en) 2016-03-25 2023-03-07 Editas Medicine, Inc. Genome editing systems comprising repair-modulating enzyme molecules and methods of their use
WO2017180694A1 (en) 2016-04-13 2017-10-19 Editas Medicine, Inc. Cas9 fusion molecules gene editing systems, and methods of use thereof
CA3026112A1 (en) * 2016-04-19 2017-10-26 The Broad Institute, Inc. Cpf1 complexes with reduced indel activity
EP3445848A1 (en) * 2016-04-19 2019-02-27 The Broad Institute, Inc. Novel crispr enzymes and systems
AU2017257274B2 (en) 2016-04-19 2023-07-13 Massachusetts Institute Of Technology Novel CRISPR enzymes and systems
EP3450570B1 (en) * 2016-04-28 2021-03-10 Industry-Academic Cooperation Foundation, Yonsei University Method for evaluating, in vivo, activity of rna-guided nuclease in high-throughput manner
US11214789B2 (en) 2016-05-03 2022-01-04 Flodesign Sonics, Inc. Concentration and washing of particles with acoustics
US11085035B2 (en) 2016-05-03 2021-08-10 Flodesign Sonics, Inc. Therapeutic cell washing, concentration, and separation utilizing acoustophoresis
US11499158B2 (en) 2016-05-13 2022-11-15 Kaneka Corporation Method for modifying plant
EP3456181A4 (en) 2016-05-13 2019-12-25 Kaneka Corporation METHOD FOR PRODUCING A TRANSFORMED PLANT
JP2017205104A (ja) * 2016-05-13 2017-11-24 株式会社カネカ 植物のゲノム編集方法
KR20240016444A (ko) 2016-05-20 2024-02-06 리제너론 파마슈티칼스 인코포레이티드 다중 가이드 RNAs를 이용한 면역학적 내성 파괴 방법
CA3018294A1 (en) * 2016-06-01 2017-12-07 Excision Biotherapeutics, Inc. Compositions and methods of treatment for lytic and lysogenic viruses
EP3907286A1 (en) * 2016-06-02 2021-11-10 Sigma-Aldrich Co., LLC Using programmable dna binding proteins to enhance targeted genome modification
MX2018014993A (es) 2016-06-14 2019-09-06 Pioneer Hi Bred Int Uso de endonucleasa cpf1 para modificaciones de genoma de planta.
US10337051B2 (en) * 2016-06-16 2019-07-02 The Regents Of The University Of California Methods and compositions for detecting a target RNA
JP7267013B2 (ja) * 2016-06-17 2023-05-01 ザ・ブロード・インスティテュート・インコーポレイテッド Ｖｉ型ｃｒｉｓｐｒオルソログ及び系
US11293021B1 (en) 2016-06-23 2022-04-05 Inscripta, Inc. Automated cell processing methods, modules, instruments, and systems
AU2017280353B2 (en) * 2016-06-24 2021-11-11 Inscripta, Inc. Methods for generating barcoded combinatorial libraries
US10544390B2 (en) 2016-06-30 2020-01-28 Zymergen Inc. Methods for generating a bacterial hemoglobin library and uses thereof
US10544411B2 (en) 2016-06-30 2020-01-28 Zymergen Inc. Methods for generating a glucose permease library and uses thereof
CN106191057B (zh) * 2016-07-06 2018-12-25 中山大学 一种用于敲除人CYP2E1基因的sgRNA序列、CYP2E1基因缺失细胞株的构建方法及其应用
WO2018013720A1 (en) * 2016-07-12 2018-01-18 Washington University Incorporation of internal polya-encoded poly-lysine sequence tags and their variations for the tunable control of protein synthesis in bacterial and eukaryotic cells
CA3030587A1 (en) * 2016-07-15 2018-01-18 Salk Institute For Biological Studies Methods and compositions for genome editing in non-dividing cells
EP3487523B1 (en) * 2016-07-19 2023-09-06 Duke University Therapeutic applications of cpf1-based genome editing
US20190247436A1 (en) 2016-07-21 2019-08-15 Maxcyte, Inc. Methods and compositions for modifying genomic dna
US11168313B2 (en) 2016-07-26 2021-11-09 The General Hospital Corporation Variants of CRISPR from Prevotella and Francisella 1 (Cpf1)
US11078481B1 (en) 2016-08-03 2021-08-03 KSQ Therapeutics, Inc. Methods for screening for cancer targets
AU2017306676B2 (en) 2016-08-03 2024-02-22 President And Fellows Of Harvard College Adenosine nucleobase editors and uses thereof
AU2017308889B2 (en) 2016-08-09 2023-11-09 President And Fellows Of Harvard College Programmable Cas9-recombinase fusion proteins and uses thereof
US11905549B2 (en) * 2016-08-10 2024-02-20 Genahead Bio, Inc. Method for modifying target site in genome of eukaryotic cell, and method for detecting presence or absence of nucleic acid sequence to be detected at target site
CN110312799A (zh) * 2016-08-17 2019-10-08 博德研究所 新型crispr酶和系统
US11352647B2 (en) 2016-08-17 2022-06-07 The Broad Institute, Inc. Crispr enzymes and systems
US11542509B2 (en) 2016-08-24 2023-01-03 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
US11078483B1 (en) 2016-09-02 2021-08-03 KSQ Therapeutics, Inc. Methods for measuring and improving CRISPR reagent function
US10650621B1 (en) 2016-09-13 2020-05-12 Iocurrents, Inc. Interfacing with a vehicular controller area network
US20190225974A1 (en) 2016-09-23 2019-07-25 BASF Agricultural Solutions Seed US LLC Targeted genome optimization in plants
WO2018064371A1 (en) 2016-09-30 2018-04-05 The Regents Of The University Of California Rna-guided nucleic acid modifying enzymes and methods of use thereof
WO2018064352A1 (en) * 2016-09-30 2018-04-05 The Regents Of The University Of California Rna-guided nucleic acid modifying enzymes and methods of use thereof
MX2019003768A (es) 2016-10-03 2019-06-24 Juno Therapeutics Inc Moleculas de enlace especificas de hpv.
EP3757120B8 (en) 2016-10-04 2022-06-15 Precision Biosciences, Inc. Co-stimulatory domains for use in genetically-modified cells
CN110462034A (zh) 2016-10-07 2019-11-15 综合Dna技术公司 化脓链球菌cas9突变基因和由其编码的多肽
US11242542B2 (en) 2016-10-07 2022-02-08 Integrated Dna Technologies, Inc. S. pyogenes Cas9 mutant genes and polypeptides encoded by same
WO2018071672A1 (en) * 2016-10-12 2018-04-19 The Regents Of The University Of Colorado Novel engineered and chimeric nucleases
SG11201903089RA (en) 2016-10-14 2019-05-30 Harvard College Aav delivery of nucleobase editors
CN110290813A (zh) * 2016-10-14 2019-09-27 通用医疗公司 表观遗传学调控的位点特异性核酸酶
WO2018075664A1 (en) 2016-10-18 2018-04-26 Regents Of The University Of Minnesota Tumor infiltrating lymphocytes and methods of therapy
EP4256951A3 (en) 2016-11-04 2023-12-06 Flagship Pioneering Innovations V. Inc. Novel plant cells, plants, and seeds
CN106591335A (zh) * 2016-11-08 2017-04-26 安徽省农业科学院水稻研究所 一种密码子植物化改造的LbCpf1基因及其应用
JP2019535287A (ja) * 2016-11-22 2019-12-12 インテグレイテツド・デイー・エヌ・エイ・テクノロジーズ・インコーポレイテツド Ｃｒｉｓｐｒ／ｃｐｆ１システム及び方法
US20200046854A1 (en) * 2016-11-28 2020-02-13 The Board Of Regents Of The University Of Texas System Prevention of muscular dystrophy by crispr/cpf1-mediated gene editing
WO2018099475A1 (zh) * 2016-12-01 2018-06-07 中国科学院上海生命科学研究院 基于Cpf1的植物基因组定点编辑方法
WO2018107103A1 (en) * 2016-12-09 2018-06-14 The Broad Institute, Inc. Crispr-systems for modifying a trait of interest in a plant
US11293022B2 (en) 2016-12-12 2022-04-05 Integrated Dna Technologies, Inc. Genome editing enhancement
US10975388B2 (en) 2016-12-14 2021-04-13 Ligandal, Inc. Methods and compositions for nucleic acid and protein payload delivery
EP3555631A4 (en) * 2016-12-19 2020-08-12 The UAB Research Foundation MASS SPECTROMETRIC STANDARDS FOR HEMOGLOBIN BETA AND HEMOGLOBIN BETA SICKLE AND USES THEREOF
EP3557981A1 (en) * 2016-12-22 2019-10-30 Keygene N.V. Method for targeted alteration of duplex dna
WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
WO2018125964A1 (en) * 2016-12-28 2018-07-05 Ionis Pharmaceuticals, Inc. Modified crispr rna and uses thereof
US11859219B1 (en) 2016-12-30 2024-01-02 Flagship Pioneering Innovations V, Inc. Methods of altering a target nucleotide sequence with an RNA-guided nuclease and a single guide RNA
CN108265068B (zh) * 2016-12-31 2021-06-15 江苏众红生物工程创药研究院有限公司 重组精氨酸脱亚胺酶及其产业化制备方法和应用
ES2949801T3 (es) 2017-01-09 2023-10-03 Whitehead Inst Biomedical Res Métodos para alterar la expresión génica mediante la perturbación de multímeros de factores de transcripción que estructuran bucles reguladores
GB201700380D0 (en) * 2017-01-10 2017-02-22 Plant Bioscience Ltd Methods of increasing seed yield
JP2020513783A (ja) * 2017-01-18 2020-05-21 エクシジョン バイオセラピューティクス インコーポレイテッド Ｃｒｉｓｐｒ
SG11201906795SA (en) 2017-01-28 2019-08-27 Inari Agriculture Inc Novel plant cells, plants, and seeds
DK3574101T3 (da) 2017-01-30 2023-07-31 Kws Saat Se & Co Kgaa Reparationsskabelonbinding til endonukleaser til genommodifikation
WO2018148511A1 (en) * 2017-02-10 2018-08-16 Zymergen Inc. A modular universal plasmid design strategy for the assembly and editing of multiple dna constructs for multiple hosts
JP7181528B2 (ja) * 2017-02-10 2022-12-01 国立研究開発法人農業・食品産業技術総合研究機構 ２－オキソグルタル酸依存的に４－ｈｐｐｄ阻害剤を酸化する触媒活性が高められたｈｓｌタンパク質の製造方法
WO2018149915A1 (en) * 2017-02-15 2018-08-23 Keygene N.V. Methods of targeted genetic alteration in plant cells
CN106868031A (zh) * 2017-02-24 2017-06-20 北京大学 一种基于分级组装的多个sgRNA串联并行表达的克隆方法及应用
CN106947779B (zh) * 2017-02-28 2021-02-26 中国农业大学 利用乳腺生物反应器提高乳汁中褪黑素含量的方法
US10687520B2 (en) 2017-03-07 2020-06-23 The Board Of Regents Of The University Of Texas System Generation and correction of a humanized mouse model with a deletion of dystrophin exon 44
WO2018165504A1 (en) 2017-03-09 2018-09-13 President And Fellows Of Harvard College Suppression of pain by gene editing
WO2018165631A1 (en) * 2017-03-09 2018-09-13 President And Fellows Of Harvard College Cancer vaccine
US11542496B2 (en) 2017-03-10 2023-01-03 President And Fellows Of Harvard College Cytosine to guanine base editor
CN106978428A (zh) * 2017-03-15 2017-07-25 上海吐露港生物科技有限公司 一种Cas蛋白特异结合靶标DNA、调控靶标基因转录的方法及试剂盒
KR102185464B1 (ko) * 2017-03-15 2020-12-03 매사추세츠 인스티튜트 오브 테크놀로지 신규 cas13b 오르소로그 crispr 효소 및 시스템
WO2018175712A1 (en) * 2017-03-23 2018-09-27 The Government Of The United States Of America As Represented By The Secretary Of The Army Recombinant adeno-associated virus-paraoxonase 1-ifii particles and the methods of making and using thereof
IL306092A (en) 2017-03-23 2023-11-01 Harvard College Nucleic base editors that include nucleic acid programmable DNA binding proteins
WO2018183908A1 (en) 2017-03-31 2018-10-04 Dana-Farber Cancer Institute, Inc. Compositions and methods for treating ovarian tumors
CN108690845B (zh) * 2017-04-10 2021-04-27 中国科学院动物研究所 基因组编辑系统和方法
US20180362590A1 (en) * 2017-04-14 2018-12-20 Synthetic Genomics, Inc. Polypeptides with type v crispr activity and uses thereof
WO2018191663A1 (en) * 2017-04-14 2018-10-18 Monsanto Technology Llc Methods and compositions for herbicide tolerance in plants
WO2018195545A2 (en) * 2017-04-21 2018-10-25 The General Hospital Corporation Variants of cpf1 (cas12a) with altered pam specificity
JP7379160B2 (ja) * 2017-04-21 2023-11-14 ザ ジェネラル ホスピタル コーポレイション ＣＲＩＳＰＲ－Ｃｐｆ１を使用する誘導性で調整可能な多重ヒト遺伝子制御
EP3615672A1 (en) 2017-04-28 2020-03-04 Editas Medicine, Inc. Methods and systems for analyzing guide rna molecules
CA3062165A1 (en) * 2017-05-04 2018-11-08 The Trustees Of The University Of Pennsylvania Compositions and methods for gene editing in t cells using crispr/cpf1
JP7170666B2 (ja) 2017-05-08 2022-11-14 プレシジョン バイオサイエンシズ，インク． 操作された抗原受容体をコードする核酸分子及び阻害性核酸分子、並びにそれらの使用方法
WO2019103857A1 (en) 2017-11-22 2019-05-31 Iovance Biotherapeutics, Inc. Expansion of peripheral blood lymphocytes (pbls) from peripheral blood
WO2018209320A1 (en) 2017-05-12 2018-11-15 President And Fellows Of Harvard College Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation
AU2018270088A1 (en) 2017-05-18 2020-01-16 Massachusetts Institute Of Technology Systems, methods, and compositions for targeted nucleic acid editing
US11046969B2 (en) * 2017-05-24 2021-06-29 Epiplanta Biotech Ltd. Transgenic plant and the method for producing the same
MA48797A (fr) * 2017-05-25 2020-04-08 Bluebird Bio Inc Variants de l'endonucléase cblb , compositions et procédés d'utilisation
US11603544B2 (en) 2017-06-05 2023-03-14 Fred Hutchinson Cancer Center Genomic safe harbors for genetic therapies in human stem cells and engineered nanoparticles to provide targeted genetic therapies
KR20200026878A (ko) 2017-06-06 2020-03-11 지머젠 인코포레이티드 균류 균주를 개량하기 위한 htp 게놈 공학 플랫폼
EP3878961A1 (en) 2017-06-06 2021-09-15 Zymergen, Inc. A htp genomic engineering platform for improving escherichia coli
EP3635104A1 (en) 2017-06-09 2020-04-15 Editas Medicine, Inc. Engineered cas9 nucleases
RU2020100074A (ru) 2017-06-13 2021-08-03 Флэгшип Пайониринг Инновейшнз V, Инк. Композиции, содержащие куроны, и пути их применения
WO2018232195A1 (en) 2017-06-14 2018-12-20 The Broad Institute, Inc. Compositions and methods targeting complement component 3 for inhibiting tumor growth
WO2018236548A1 (en) 2017-06-23 2018-12-27 Inscripta, Inc. NUCLEIC ACID GUIDED NUCLEASES
US10011849B1 (en) 2017-06-23 2018-07-03 Inscripta, Inc. Nucleic acid-guided nucleases
US9982279B1 (en) 2017-06-23 2018-05-29 Inscripta, Inc. Nucleic acid-guided nucleases
AU2018290843A1 (en) 2017-06-26 2020-01-16 Massachusetts Institute Of Technology CRISPR/Cas-adenine deaminase based compositions, systems, and methods for targeted nucleic acid editing
CN107312790A (zh) * 2017-06-26 2017-11-03 中国科学技术大学 一种可编程的多位点特异性的转录抑制系统及其应用
EP3848459A1 (en) 2017-06-30 2021-07-14 Inscripta, Inc. Automated cell processing methods, modules, instruments and systems
US10392616B2 (en) 2017-06-30 2019-08-27 Arbor Biotechnologies, Inc. CRISPR RNA targeting enzymes and systems and uses thereof
WO2019006418A2 (en) 2017-06-30 2019-01-03 Intima Bioscience, Inc. ADENO-ASSOCIATED VIRAL VECTORS FOR GENE THERAPY
KR102007457B1 (ko) * 2017-06-30 2019-08-05 동국대학교 산학협력단 Cas9 단백질, 가이드 RNA 및 양친매성 펩타이드를 포함하는 나노복합체 및 이의 용도
EP3645038A1 (en) * 2017-06-30 2020-05-06 Precision Biosciences, Inc. Genetically-modified t cells comprising a modified intron in the t cell receptor alpha gene
US20200165594A1 (en) * 2017-07-07 2020-05-28 The Broad Institute, Inc. Crispr system based antiviral therapy
WO2019014564A1 (en) 2017-07-14 2019-01-17 Editas Medicine, Inc. SYSTEMS AND METHODS OF TARGETED INTEGRATION AND GENOME EDITING AND DETECTION THEREOF WITH INTEGRATED PRIMING SITES
CN112501254A (zh) * 2017-07-14 2021-03-16 上海吐露港生物科技有限公司 一种Cas蛋白的用途及靶标核酸分子的检测方法和试剂盒
US20200231975A1 (en) * 2017-07-17 2020-07-23 The Broad Institute, Inc. Novel type vi crispr orthologs and systems
US11041154B2 (en) 2017-07-21 2021-06-22 Arizona Board Of Regents On Behalf Of Arizona State University CRISPR fluorescent guide RNA (fgRNA) to understanding gRNAs expressed from pol II promotors
JP7159288B2 (ja) 2017-07-28 2022-10-24 ローカス アイピー カンパニー、エルエルシー 皮膚、毛髪及び頭皮の健康を改善する酵母ベースのマスク
JP2020534795A (ja) 2017-07-28 2020-12-03 プレジデント アンド フェローズ オブ ハーバード カレッジ ファージによって支援される連続的進化（ｐａｃｅ）を用いて塩基編集因子を進化させるための方法および組成物
AU2018309714A1 (en) 2017-07-31 2020-01-30 Regeneron Pharmaceuticals, Inc. Assessment of CRISPR/Cas-induced recombination with an exogenous donor nucleic acid in vivo
CA3067872A1 (en) 2017-07-31 2019-02-07 Regeneron Pharmaceuticals, Inc. Cas-transgenic mouse embryonic stem cells and mice and uses thereof
US11021719B2 (en) 2017-07-31 2021-06-01 Regeneron Pharmaceuticals, Inc. Methods and compositions for assessing CRISPER/Cas-mediated disruption or excision and CRISPR/Cas-induced recombination with an exogenous donor nucleic acid in vivo
EP3662065A4 (en) * 2017-08-04 2021-04-21 Syngenta Participations AG METHODS AND COMPOSITIONS FOR TARGETED GENOMIC INSERTION
ES2960390T3 (es) 2017-08-09 2024-03-04 Ricetec Inc Composiciones y métodos para modificar genomas
US11970720B2 (en) 2017-08-22 2024-04-30 Salk Institute For Biological Studies RNA targeting methods and compositions
US10476825B2 (en) 2017-08-22 2019-11-12 Salk Institue for Biological Studies RNA targeting methods and compositions
JP2020532967A (ja) 2017-08-23 2020-11-19 ザ ジェネラル ホスピタル コーポレイション 変更されたＰＡＭ特異性を有する遺伝子操作されたＣＲＩＳＰＲ−Ｃａｓ９ヌクレアーゼ
US10738327B2 (en) 2017-08-28 2020-08-11 Inscripta, Inc. Electroporation cuvettes for automation
WO2019139645A2 (en) 2017-08-30 2019-07-18 President And Fellows Of Harvard College High efficiency base editors comprising gam
EP3676378A4 (en) * 2017-08-31 2021-06-09 The New York Genome Center PROCEDURES AND COMPOSITIONS USING CRIMP-CPF1 AND PAIRED GUIDE-CRISPR-RNA FOR PROGRAMMABLE GENOMIC DELETIONS
CN111511388A (zh) 2017-09-21 2020-08-07 博德研究所 用于靶向核酸编辑的系统、方法和组合物
CN111163633B (zh) 2017-09-29 2022-09-09 瑞泽恩制药公司 包含人源化ttr基因座的非人类动物及其使用方法
US11845951B2 (en) 2017-09-29 2023-12-19 Toolgen Incorporated Gene manipulation for treatment of retinal dysfunction disorder
CN111372650A (zh) 2017-09-30 2020-07-03 因思科瑞普特公司 流通式电穿孔仪器
US20200239544A1 (en) 2017-10-03 2020-07-30 Precision Biosciences, Inc. Modified epidermal growth factor receptor peptides for use in genetically-modified cells
EP4215543A3 (en) 2017-10-03 2023-10-11 Juno Therapeutics, Inc. Hpv-specific binding molecules
US11633732B2 (en) 2017-10-04 2023-04-25 The Broad Institute, Inc. CRISPR effector system based diagnostics
US20190352652A1 (en) * 2017-10-04 2019-11-21 The Broad Institute, Inc. Crispr-systems for modifying a trait of interest in a plant
US11795443B2 (en) 2017-10-16 2023-10-24 The Broad Institute, Inc. Uses of adenosine base editors
MX2020004063A (es) 2017-10-20 2020-10-05 Hutchinson Fred Cancer Res Sistemas y metodos para producir celulas b modificadas geneticamente para expresar anticuerpos seleccionados.
CA3080493A1 (en) 2017-11-01 2019-05-09 The Regents Of The University Of California Casz compositions and methods of use
US20200299658A1 (en) 2017-11-01 2020-09-24 Precision Biosciences, Inc. Engineered nucleases that target human and canine factor viii genes as a treatment for hemophilia a
US11970719B2 (en) 2017-11-01 2024-04-30 The Regents Of The University Of California Class 2 CRISPR/Cas compositions and methods of use
WO2019090173A1 (en) 2017-11-02 2019-05-09 Arbor Biotechnologies, Inc. Novel crispr-associated transposon systems and components
GB2568255A (en) * 2017-11-08 2019-05-15 Evox Therapeutics Ltd Exosomes comprising RNA therapeutics
JP7423520B2 (ja) * 2017-11-16 2024-01-29 アストラゼネカ・アクチエボラーグ Ｃａｓ９ベースノックイン方針の効力を改善するための組成物及び方法
SG11202004457XA (en) 2017-11-17 2020-06-29 Iovance Biotherapeutics Inc Til expansion from fine needle aspirates and small biopsies
US10253365B1 (en) * 2017-11-22 2019-04-09 The Regents Of The University Of California Type V CRISPR/Cas effector proteins for cleaving ssDNAs and detecting target DNAs
US20200332287A1 (en) * 2017-11-28 2020-10-22 Emendobio Inc. Differential knockout of an allele of a heterozygous fibrinogen alpha chain (fga) gene
WO2019113506A1 (en) 2017-12-07 2019-06-13 The Broad Institute, Inc. Methods and compositions for multiplexing single cell and single nuclei sequencing
MX2020005939A (es) 2017-12-08 2020-08-24 Synthetic Genomics Inc Mejoramiento de la productividad de lipidos de algas a traves de la modificacion genetica de una proteina que contiene un dominio tpr.
US11203752B2 (en) * 2017-12-11 2021-12-21 Pioneer Hi-Bred International, Inc. Compositions and methods of modifying a plant genome to produce a MS9, MS22, MS26, or MS45 male-sterile plant
BR112020009889A2 (pt) 2017-12-14 2020-11-03 Flodesign Sonics, Inc. acionador e controlador de transdutor acústico
CA3078682A1 (en) 2017-12-15 2019-06-20 Dana-Farber Cancer Institute, Inc. Stabilized peptide-mediated targeted protein degradation
CA3085914A1 (en) 2017-12-22 2019-06-27 G+Flas Life Sciences Chimeric genome engineering molecules and methods
SG11202005173SA (en) * 2017-12-22 2020-06-29 Fate Therapeutics Inc Enhanced immune effector cells and use thereof
WO2019138083A1 (en) 2018-01-12 2019-07-18 Basf Se Gene underlying the number of spikelets per spike qtl in wheat on chromosome 7a
CN111742051A (zh) * 2018-01-23 2020-10-02 基础科学研究院 延伸的单向导rna及其用途
US11220694B1 (en) * 2018-01-29 2022-01-11 Inari Agriculture, Inc. Rice cells and rice plants
US11926835B1 (en) 2018-01-29 2024-03-12 Inari Agriculture Technology, Inc. Methods for efficient tomato genome editing
US20210047653A1 (en) * 2018-01-30 2021-02-18 The University Of Memphis Research Foundation Compositions and methods for regulating a biological process
US20190241911A1 (en) * 2018-02-06 2019-08-08 Ohio State Innovation Foundation Engineered guide rna and uses thereof
CN111788307A (zh) * 2018-03-12 2020-10-16 南京北恒生物科技有限公司 工程化的嵌合向导rna及其用途
EP3765616B1 (en) 2018-03-14 2023-07-19 Arbor Biotechnologies, Inc. Novel crispr dna and rna targeting enzymes and systems
EP4257696A3 (en) 2018-03-14 2024-01-24 Arbor Biotechnologies, Inc. Novel crispr dna targeting enzymes and systems
CA3093968A1 (en) * 2018-03-15 2019-09-19 KSQ Therapeutics, Inc. Gene-regulating compositions and methods for improved immunotherapy
CN108486146B (zh) * 2018-03-16 2021-02-19 中国农业科学院作物科学研究所 LbCpf1-RR突变体用于CRISPR/Cpf1系统在植物基因编辑中的应用
AU2019239880B2 (en) * 2018-03-19 2023-11-30 Regeneron Pharmaceuticals, Inc. Transcription modulation in animals using CRISPR/Cas systems
EP3546582A1 (en) 2018-03-26 2019-10-02 KWS SAAT SE & Co. KGaA Promoter activating elements
WO2019191495A1 (en) * 2018-03-29 2019-10-03 Fate Therapeutics, Inc. Engineered immune effector cells and use thereof
WO2019190874A1 (en) 2018-03-29 2019-10-03 Inscripta, Inc. Automated control of cell growth rates for induction and transformation
BR112020020245A2 (pt) 2018-04-05 2021-04-06 Editas Medicine, Inc. Métodos de produzir células expressando um receptor recombinante e composições relacionadas
RU2020136054A (ru) 2018-04-05 2022-05-06 Джуно Терапьютикс, Инк. Т-клетки, экспрессирующие рекомбинантный рецептор, соответствующие полинуклеотиды и способы
IL304025A (en) 2018-04-12 2023-08-01 Prec Biosciences Inc Optimized engineered nucleases with specificity for the human T cell receptor alpha constant region gene
WO2019200004A1 (en) 2018-04-13 2019-10-17 Inscripta, Inc. Automated cell processing instruments comprising reagent cartridges
CN112166194A (zh) * 2018-04-23 2021-01-01 杜克大学 通过dna甲基化的靶向编辑下调snca的表达
US10501738B2 (en) 2018-04-24 2019-12-10 Inscripta, Inc. Automated instrumentation for production of peptide libraries
US10858761B2 (en) 2018-04-24 2020-12-08 Inscripta, Inc. Nucleic acid-guided editing of exogenous polynucleotides in heterologous cells
US10526598B2 (en) 2018-04-24 2020-01-07 Inscripta, Inc. Methods for identifying T-cell receptor antigens
EP3560330B1 (en) 2018-04-24 2022-06-15 KWS SAAT SE & Co. KGaA Plants with improved digestibility and marker haplotypes
US11957695B2 (en) 2018-04-26 2024-04-16 The Broad Institute, Inc. Methods and compositions targeting glucocorticoid signaling for modulating immune responses
BR112020021660A2 (pt) 2018-04-27 2021-02-02 Iovance Biotherapeutics, Inc. métodos para expandir linfócitos infiltrantes de tumor e para tratar um indivíduo com câncer, população de linfócitos infiltrantes de tumor, e, composição de criopreservação
CN109593763B (zh) * 2018-04-27 2021-10-29 四川大学华西医院 一种FnCpf1介导的体外DNA编辑试剂盒
US20210198664A1 (en) 2018-05-16 2021-07-01 Arbor Biotechnologies, Inc. Novel crispr-associated systems and components
EP3802826A1 (en) * 2018-05-24 2021-04-14 CRISPR Therapeutics AG Methods and compositions for efficient gene deletion
EP3833680A4 (en) * 2018-05-30 2022-05-25 The Regents of the University of California GENE EDIT OF MONOGENIC DISEASES IN HUMAN HEMATOPOIETIC STEM CELLS - CORRECTION OF X-LINKED HYPER-IGM SYNDROME (XHIM)
AU2019282094A1 (en) * 2018-06-04 2021-01-07 University Of Copenhagen Mutant Cpf1 endonucleases
US11866719B1 (en) 2018-06-04 2024-01-09 Inari Agriculture Technology, Inc. Heterologous integration of regulatory elements to alter gene expression in wheat cells and wheat plants
SG11202011975WA (en) 2018-06-05 2020-12-30 Lifeedit Inc Rna-guided nucleases and active fragments and variants thereof and methods of use
US20210127649A1 (en) * 2018-06-07 2021-05-06 Korea Research Institute Of Bioscience And Biotechnology Transgenic mouse for aglycosylated antibody production and use of aglycosylated antibody produced therefrom
EP3802825A1 (en) 2018-06-08 2021-04-14 Intellia Therapeutics, Inc. Compositions and methods for immunooncology
US10227576B1 (en) 2018-06-13 2019-03-12 Caribou Biosciences, Inc. Engineered cascade components and cascade complexes
WO2019237395A1 (zh) * 2018-06-16 2019-12-19 深圳市博奥康生物科技有限公司 一种应用CRISPR/Cas9系统靶向敲除人CD357基因的方法
WO2019237380A1 (zh) * 2018-06-16 2019-12-19 深圳市博奥康生物科技有限公司 一种基于慢病毒的 CRISPR/Cpf1 基因编辑载体及其应用
WO2020006067A1 (en) 2018-06-26 2020-01-02 The Broad Institute, Inc. Crispr double nickase based amplification compositions, systems, and methods
KR20210040943A (ko) 2018-06-26 2021-04-14 매사추세츠 인스티튜트 오브 테크놀로지 Crispr 이펙터 시스템 기반 증폭 방법, 시스템, 및 진단
EP3521436A1 (en) 2018-06-27 2019-08-07 VIB vzw Complex breeding in plants
AU2019292919A1 (en) 2018-06-30 2021-03-11 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
WO2020018142A1 (en) 2018-07-16 2020-01-23 Arbor Biotechnologies, Inc. Novel crispr dna targeting enzymes and systems
EP3830301A1 (en) 2018-08-01 2021-06-09 Mammoth Biosciences, Inc. Programmable nuclease compositions and methods of use thereof
MX2021001578A (es) * 2018-08-09 2021-06-15 G Flas Life Sciences Proteina asociada a crispr novedosa y uso de la misma.
WO2020030984A2 (en) 2018-08-09 2020-02-13 G+Flas Life Sciences Compositions and methods for genome engineering with cas12a proteins
US10532324B1 (en) 2018-08-14 2020-01-14 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
US11142740B2 (en) 2018-08-14 2021-10-12 Inscripta, Inc. Detection of nuclease edited sequences in automated modules and instruments
US10752874B2 (en) 2018-08-14 2020-08-25 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
MA53445A (fr) * 2018-08-24 2021-12-01 Flagship Pioneering Innovations Vi Llc Procédés et compositions pour la modification de plantes
AR116016A1 (es) * 2018-08-24 2021-03-25 Flagship Pioneering Innovations Vi Llc Métodos para fabricar paquetes mensajeros vegetales
US11965154B2 (en) 2018-08-30 2024-04-23 Inscripta, Inc. Detection of nuclease edited sequences in automated modules and instruments
US11479762B1 (en) 2018-08-31 2022-10-25 Inari Agriculture Technology, Inc. Compositions, systems, and methods for genome editing
WO2020051507A1 (en) 2018-09-06 2020-03-12 The Broad Institute, Inc. Nucleic acid assemblies for use in targeted delivery
CN109402115B (zh) * 2018-09-06 2024-02-02 广州普世利华科技有限公司 靶向Rett突变基因RNA的gRNA及Rett突变基因的检测方法、检测试剂盒
WO2020049158A1 (en) 2018-09-07 2020-03-12 Astrazeneca Ab Compositions and methods for improved nucleases
US20220098613A1 (en) 2018-09-12 2022-03-31 Fred Hutchinson Cancer Research Center Reducing cd33 expression to selectively protect therapeutic cells
WO2020055941A1 (en) * 2018-09-13 2020-03-19 Excision Biotherapeutics, Inc. Compositions and methods for excision with single grna
US11459552B2 (en) * 2018-09-13 2022-10-04 The Board Of Regents Of The University Of Oklahoma Variant CAS12 proteins with improved dna cleavage selectivity and methods of use
CA3115534A1 (en) 2018-10-22 2020-04-30 Inscripta, Inc. Engineered nucleic acid-guided nucleases
US11214781B2 (en) 2018-10-22 2022-01-04 Inscripta, Inc. Engineered enzyme
CN111100876A (zh) * 2018-10-25 2020-05-05 立沃生物科技（深圳）有限公司 CRISPR-Cas9特异性敲除FAH基因的方法及特异性sgRNA
US11407995B1 (en) 2018-10-26 2022-08-09 Inari Agriculture Technology, Inc. RNA-guided nucleases and DNA binding proteins
WO2020092057A1 (en) 2018-10-30 2020-05-07 Yale University Compositions and methods for rapid and modular generation of chimeric antigen receptor t cells
SG11202104409YA (en) 2018-10-31 2021-05-28 Zymergen Inc Multiplexed deterministic assembly of dna libraries
US20220333089A1 (en) * 2018-11-01 2022-10-20 The University Of Tokyo Split CPF1 Protein
US11434477B1 (en) 2018-11-02 2022-09-06 Inari Agriculture Technology, Inc. RNA-guided nucleases and DNA binding proteins
US20230039976A1 (en) 2018-11-05 2023-02-09 Iovance Biotherapeutics, Inc. Selection of improved tumor reactive t-cells
AU2019374761A1 (en) 2018-11-05 2021-06-10 Iovance Biotherapeutics, Inc. Processes for production of tumor infiltrating lymphocytes and uses of the same in immunotherapy
US20220033775A1 (en) 2018-11-05 2022-02-03 Iovance Biotherapeutics, Inc. Expansion of tils utilizing akt pathways inhibitors
BR112021008549A2 (pt) 2018-11-05 2022-01-04 Iovance Biotherapeutics Inc Método de tratamento de carcinoma pulmonar de células não pequenas com uma população de linfócitos infiltrantes de tumor
CN109385451B (zh) * 2018-11-07 2020-07-07 中国海洋大学 一种牡蛎CRISPR/Cas9基因编辑方法
EP3880826A4 (en) * 2018-11-07 2022-08-24 Akouos, Inc. USE OF ADENO-ASSOCIATED VIRAL VECTORS TO CORRECT GENE DEFECTS/EXPRESS PROTEINS IN CILIATE AND SUPPORTING CELLS IN THE INNER EAR
CA3119972A1 (en) * 2018-11-14 2020-05-22 Cameron MYHRVOLD Crispr system based droplet diagnostic systems and methods
CN113015798B (zh) * 2018-11-15 2023-01-10 中国农业大学 CRISPR-Cas12a酶和系统
JP7460178B2 (ja) * 2018-11-15 2024-04-02 中国▲農▼▲業▼大学 ＣＲＩＳＰＲ－Ｃａｓ１２ｊ酵素およびシステム
CN109536506B (zh) * 2018-12-10 2022-03-22 潍坊科技学院 一种调控日本沼虾生长发育的类胰岛素受体基因及其应用
US11166996B2 (en) 2018-12-12 2021-11-09 Flagship Pioneering Innovations V, Inc. Anellovirus compositions and methods of use
CN109680053A (zh) * 2018-12-12 2019-04-26 广州普世利华科技有限公司 新型SsCas12a蛋白在核酸检测方面的应用
CN109593743A (zh) * 2018-12-12 2019-04-09 广州普世利华科技有限公司 新型CRISPR/ScCas12a蛋白及其制备方法
CN109666662A (zh) * 2018-12-12 2019-04-23 广州普世利华科技有限公司 新型ScCas12a在核酸检测方面的应用
WO2020124050A1 (en) 2018-12-13 2020-06-18 The Broad Institute, Inc. Tiled assays using crispr-cas based detection
CA3124110A1 (en) 2018-12-17 2020-06-25 The Broad Institute, Inc. Crispr-associated transposase systems and methods of use thereof
EP3898949A1 (en) 2018-12-19 2021-10-27 Iovance Biotherapeutics, Inc. Methods of expanding tumor infiltrating lymphocytes using engineered cytokine receptor pairs and uses thereof
SG11202105189RA (en) 2018-12-20 2021-06-29 Regeneron Pharma Nuclease-mediated repeat expansion
WO2020132659A1 (en) 2018-12-21 2020-06-25 Precision Biosciences, Inc. Genetic modification of the hydroxyacid oxidase 1 gene for treatment of primary hyperoxaluria
MX2021007835A (es) 2018-12-27 2021-08-11 Lifeedit Therapeutics Inc Polipeptidos utiles para edicion de genes y metodos de uso.
WO2020142754A2 (en) 2019-01-04 2020-07-09 Mammoth Biosciences, Inc. Programmable nuclease improvements and compositions and methods for nucleic acid amplification and detection
US11739156B2 (en) 2019-01-06 2023-08-29 The Broad Institute, Inc. Massachusetts Institute of Technology Methods and compositions for overcoming immunosuppression
CN109799295A (zh) * 2019-01-28 2019-05-24 江南大学 一种用于秀丽隐杆线虫代谢组学分析的样品前处理方法
US20220145330A1 (en) 2019-02-10 2022-05-12 The J. David Gladstone Institutes, a testamentary trust established under the Will of J. David Glads Modified mitochondrion and methods of use thereof
EA202192233A1 (ru) 2019-02-12 2021-11-11 Университа' Дельи Студи Ди Тренто МОЛЕКУЛЫ ГИДОВОЙ РНК Cas12a И ИХ ПРИМЕНЕНИЕ
US20220235347A1 (en) 2019-02-13 2022-07-28 Beam Therapeutics Inc. Compositions and methods for treating hemoglobinopathies
CN114190093A (zh) * 2019-02-13 2022-03-15 比姆医疗股份有限公司 使用腺苷酸脱氨酶碱基编辑器破坏疾病相关基因的剪接受体位点，包括用于治疗遗传性疾病
JP7390385B2 (ja) * 2019-02-13 2023-12-01 プロバイオジェン アーゲー 挿入部位選択特性が向上したトランスポザーゼ
AU2020226864B2 (en) * 2019-02-22 2023-09-28 Integrated Dna Technologies, Inc. Lachnospiraceae Bacterium ND2006 Cas12a mutant genes and polypeptides encoded by same
CN109975543B (zh) * 2019-03-01 2022-02-11 中国疾病预防控制中心传染病预防控制所 分枝杆菌Ku蛋白的应用
KR20210136050A (ko) 2019-03-01 2021-11-16 이오반스 바이오테라퓨틱스, 인크. 액상 종양으로부터의 종양 침윤 림프구의 확장 및 그의 치료 용도
JP2022524043A (ja) 2019-03-08 2022-04-27 ザイマージェン インコーポレイテッド 微生物の反復ゲノム編集
US11053515B2 (en) 2019-03-08 2021-07-06 Zymergen Inc. Pooled genome editing in microbes
CN113825834A (zh) 2019-03-11 2021-12-21 索伦托药业有限公司 使用rna引导的核酸内切酶进行dna构建体整合的改进工艺
WO2020186231A2 (en) 2019-03-14 2020-09-17 The Broad Institute, Inc. Crispr effector system based multiplex diagnostics
AU2020241856A1 (en) 2019-03-18 2021-08-12 Regeneron Pharmaceuticals, Inc. CRISPR/Cas dropout screening platform to reveal genetic vulnerabilities associated with tau aggregation
AU2020241858A1 (en) 2019-03-18 2021-08-12 Regeneron Pharmaceuticals, Inc. CRISPR/Cas screening platform to identify genetic modifiers of tau seeding or aggregation
JP2022526908A (ja) 2019-03-19 2022-05-27 ザ ブロード インスティテュート，インコーポレーテッド 編集ヌクレオチド配列を編集するための方法および組成物
US11001831B2 (en) 2019-03-25 2021-05-11 Inscripta, Inc. Simultaneous multiplex genome editing in yeast
WO2020198174A1 (en) 2019-03-25 2020-10-01 Inscripta, Inc. Simultaneous multiplex genome editing in yeast
JP2022527809A (ja) 2019-04-03 2022-06-06 リジェネロン・ファーマシューティカルズ・インコーポレイテッド 抗体コード配列をセーフハーバー遺伝子座に挿入するための方法および組成物
WO2020206248A1 (en) 2019-04-03 2020-10-08 Precision Biosciences, Inc. Genetically-modified immune cells comprising a microrna-adapted shrna (shrnamir)
US11111504B2 (en) 2019-04-04 2021-09-07 Regeneron Pharmaceuticals, Inc. Methods for scarless introduction of targeted modifications into targeting vectors
SG11202108454RA (en) 2019-04-04 2021-09-29 Regeneron Pharma Non-human animals comprising a humanized coagulation factor 12 locus
US20220204994A1 (en) 2019-04-05 2022-06-30 Precision Biosciences, Inc. Methods of preparing populations of genetically-modified immune cells
US20220186263A1 (en) 2019-04-05 2022-06-16 Osaka University Method for producing knock-in cell
CN110396543A (zh) * 2019-04-30 2019-11-01 广州普世利华科技有限公司 一种肿瘤相关基因突变位点筛查方法
CN110195102A (zh) * 2019-04-30 2019-09-03 广州普世利华科技有限公司 一种β地中海贫血基因分型方法
US20220220495A1 (en) 2019-05-10 2022-07-14 Basf Se Regulatory nucleic acid molecules for enhancing gene expression in plants
EP3969607A1 (en) 2019-05-13 2022-03-23 KWS SAAT SE & Co. KGaA Drought tolerance in corn
WO2020232029A1 (en) 2019-05-13 2020-11-19 Iovance Biotherapeutics, Inc. Methods and compositions for selecting tumor infiltrating lymphocytes and uses of the same in immunotherapy
CN110172442B (zh) * 2019-05-16 2022-05-13 上海苹谱医疗科技有限公司 一种人诱导多潜能干细胞、构建方法及其用途
US20220220469A1 (en) * 2019-05-20 2022-07-14 The Broad Institute, Inc. Non-class i multi-component nucleic acid targeting systems
WO2020234468A1 (en) 2019-05-23 2020-11-26 Nomad Bioscience Gmbh Rna viral rna molecule for gene editing
AR118995A1 (es) 2019-05-25 2021-11-17 Kws Saat Se & Co Kgaa Mejorador de la inducción de haploides
EP3801011A1 (en) 2019-06-04 2021-04-14 Regeneron Pharmaceuticals, Inc. Non-human animals comprising a humanized ttr locus with a beta-slip mutation and methods of use
EP3953477A4 (en) 2019-06-06 2022-06-22 Inscripta, Inc. HARDENING FOR RECURSIVE NUCLEIC ACID-DRIVEN CELL EDITING
KR20220032050A (ko) 2019-06-07 2022-03-15 스크라이브 테라퓨틱스 인크. 조작된 casx 시스템
MX2021015122A (es) 2019-06-07 2022-04-06 Regeneron Pharma Animales no humanos que comprenden un locus de albumina humanizado.
AU2020291144A1 (en) 2019-06-13 2022-02-10 Nunhems B.V. Tomato plant producing fruit having improved ripening characteristics
CA3137765A1 (en) 2019-06-14 2020-12-17 Regeneron Pharmaceuticals, Inc. Models of tauopathy
US10907125B2 (en) 2019-06-20 2021-02-02 Inscripta, Inc. Flow through electroporation modules and instrumentation
EP3986909A4 (en) 2019-06-21 2023-08-02 Inscripta, Inc. GENOME-WIDE RATIONAL DESIGNED MUTATIONS LEADING TO INCREASED LYSINE PRODUCTION IN E. COLI
US10927385B2 (en) 2019-06-25 2021-02-23 Inscripta, Inc. Increased nucleic-acid guided cell editing in yeast
KR20220052905A (ko) 2019-06-25 2022-04-28 이나리 아그리컬쳐 테크놀로지, 인크. 개선된 상동성 의존적 수선 게놈 편집
CN112168808B (zh) * 2019-07-02 2023-01-24 深圳市第二人民医院 一种基于crispr的细胞核靶向药物递送系统
BR112021026832A2 (pt) 2019-07-02 2022-05-10 Hutchinson Fred Cancer Res Vetores ad35 recombinantes e aprimoramentos da terapia gênica relacionada
US20220259611A1 (en) 2019-07-05 2022-08-18 Limagrain Europe Method for increasing yield in plants
AU2020314969A1 (en) * 2019-07-17 2022-02-03 Fate Therapeutics, Inc. Immune effector cell engineering and use thereof
EP4004216A1 (en) 2019-07-25 2022-06-01 Precision BioSciences, Inc. Compositions and methods for sequential stacking of nucleic acid sequences into a genomic locus
US20220364159A1 (en) * 2019-07-26 2022-11-17 Mammoth Biosciences, Inc. Compositions for detection of dna and methods of use thereof
CN110358767B (zh) * 2019-07-30 2021-06-08 湖北大学 一种基于CRISPR-Cas12a系统的运动发酵单胞菌基因组编辑方法及其应用
US20220282259A1 (en) * 2019-08-02 2022-09-08 Monsanto Technology Llc Methods and compositions to promote targeted genome modifications using huh endonucleases
EP3772542A1 (en) 2019-08-07 2021-02-10 KWS SAAT SE & Co. KGaA Modifying genetic variation in crops by modulating the pachytene checkpoint protein 2
WO2021028359A1 (en) 2019-08-09 2021-02-18 Sangamo Therapeutics France Controlled expression of chimeric antigen receptors in t cells
EP4013864A1 (en) 2019-08-12 2022-06-22 Lifeedit Therapeutics, Inc. Rna-guided nucleases and active fragments and variants thereof and methods of use
CN110592014A (zh) * 2019-08-14 2019-12-20 广东美赛尔细胞生物科技有限公司 一种在nk细胞治疗中免辐照体外体内持续去除饲养细胞的方法
EP4047087A4 (en) * 2019-08-19 2023-08-23 Southern Medical University CONSTRUCTION AND APPLICATION OF HIGH-FIDELITY CRISPR/ASCPF1 MUTANTS
WO2021035054A1 (en) 2019-08-20 2021-02-25 Precision Biosciences, Inc. Lymphodepletion dosing regimens for cellular immunotherapies
WO2021035170A1 (en) 2019-08-21 2021-02-25 Precision Biosciences, Inc. Compositions and methods for tcr reprogramming using fusion proteins
CN112442515B (zh) * 2019-09-02 2023-01-10 南京启真基因工程有限公司 gRNA靶点组合在构建血友病模型猪细胞系中的应用
US20220333208A1 (en) 2019-09-03 2022-10-20 The Broad Institute, Inc. Crispr effector system based multiplex cancer diagnostics
AU2020341840A1 (en) * 2019-09-05 2022-04-14 Benson Hill, Inc. Compositions and methods for modifying genomes
CN114341166A (zh) * 2019-09-05 2022-04-12 阿伯生物技术公司 新型crispr dna靶向酶及系统
WO2021050755A1 (en) 2019-09-10 2021-03-18 Caspr Biotech Corporation Novel class 2 type ii and type v crispr-cas rna-guided endonucleases
WO2021048316A1 (en) 2019-09-12 2021-03-18 Basf Se Regulatory nucleic acid molecules for enhancing gene expression in plants
CN110628816B (zh) * 2019-09-12 2022-10-18 中国医学科学院血液病医院(中国医学科学院血液学研究所) 一种用于血友病a的双切供体及其药物组合
WO2021050940A1 (en) 2019-09-13 2021-03-18 Regeneron Pharmaceuticals, Inc. Transcription modulation in animals using crispr/cas systems delivered by lipid nanoparticles
CN110600083B (zh) * 2019-09-20 2020-05-19 中国人民解放军军事科学院军事医学研究院 基于无拼接组装wgs数据的醋酸钙—鲍曼不动杆菌复合群鉴定方法
EP4048807A1 (en) 2019-09-23 2022-08-31 Omega Therapeutics, Inc. Compositions and methods for modulating apolipoprotein b (apob) gene expression
AU2020352552A1 (en) 2019-09-23 2022-03-17 Omega Therapeutics, Inc. Compositions and methods for modulating hepatocyte nuclear factor 4-alpha (HNF4α) gene expression
JP2022550599A (ja) 2019-10-03 2022-12-02 アーティサン ディベロップメント ラブズ インコーポレイテッド 操作されたデュアルガイド核酸を有するｃｒｉｓｐｒシステム
WO2021069387A1 (en) 2019-10-07 2021-04-15 Basf Se Regulatory nucleic acid molecules for enhancing gene expression in plants
CA3157707A1 (en) * 2019-10-17 2021-04-22 Pairwise Plants Services, Inc. Variants of cas12a nucleases and methods of making and use thereof
AU2020366566A1 (en) 2019-10-17 2022-04-21 KWS SAAT SE & Co. KGaA Enhanced disease resistance of crops by downregulation of repressor genes
CA3155727A1 (en) 2019-10-25 2021-04-29 Cecile Chartier-Courtaud Gene editing of tumor infiltrating lymphocytes and uses of same in immunotherapy
WO2021081384A1 (en) * 2019-10-25 2021-04-29 Greenvenus, Llc Synthetic nucleases
US11844800B2 (en) 2019-10-30 2023-12-19 Massachusetts Institute Of Technology Methods and compositions for predicting and preventing relapse of acute lymphoblastic leukemia
US20220411479A1 (en) 2019-10-30 2022-12-29 Precision Biosciences, Inc. Cd20 chimeric antigen receptors and methods of use for immunotherapy
CA3160186A1 (en) 2019-11-05 2021-05-14 Pairwise Plants Services, Inc. Compositions and methods for rna-encoded dna-replacement of alleles
WO2021092513A1 (en) 2019-11-08 2021-05-14 Regeneron Pharmaceuticals, Inc. Crispr and aav strategies for x-linked juvenile retinoschisis therapy
CN115052980A (zh) * 2019-11-18 2022-09-13 上海蓝十字医学科学研究所 衍生自黄杆菌的基因编辑系统
WO2021102059A1 (en) 2019-11-19 2021-05-27 Inscripta, Inc. Methods for increasing observed editing in bacteria
CN110760597B (zh) * 2019-11-22 2022-08-23 西北农林科技大学 一种检测黄牛ncstn基因拷贝数变异的方法及其应用
WO2021108363A1 (en) 2019-11-25 2021-06-03 Regeneron Pharmaceuticals, Inc. Crispr/cas-mediated upregulation of humanized ttr allele
EP4065701A4 (en) * 2019-11-27 2023-11-29 Danmarks Tekniske Universitet CONSTRUCTS, COMPOSITIONS AND METHODS THEREOF WITH IMPROVED GENOMEDITING EFFICIENCY AND SPECIFICITY
US20230002455A1 (en) 2019-11-29 2023-01-05 Basf Se Increasing resistance against fungal infections in plants
AU2020396138A1 (en) 2019-12-03 2022-06-16 Basf Se Regulatory nucleic acid molecules for enhancing gene expression in plants
WO2021113788A1 (en) * 2019-12-06 2021-06-10 Pairwise Plants Services, Inc. Recruitment methods and compounds, compositions and systems for recruitment
EP4069285A1 (en) 2019-12-06 2022-10-12 Precision BioSciences, Inc. Methods for cancer immunotherapy, using lymphodepletion regimens and cd19, cd20 or bcma allogeneic car t cells
KR20220110778A (ko) 2019-12-10 2022-08-09 인스크립타 인코포레이티드 신규 mad 뉴클레아제
EP4073236A1 (en) 2019-12-11 2022-10-19 Iovance Biotherapeutics, Inc. Processes for the production of tumor infiltrating lymphocytes (tils) and methods of using the same
CN110791523B (zh) * 2019-12-13 2022-05-10 南京农业大学 一种棉花抗旱相关基因GhRCHY1及其应用
RU2720767C1 (ru) * 2019-12-13 2020-05-13 Федеральное бюджетное учреждение науки «Центральный научно-исследовательский институт эпидемиологии» Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека (ФБУН ЦНИИ Эпидемиологии Роспотребнадзора) Способ обнаружения провирусной ДНК вируса иммунодефицита человека, интегрированной в геном человека, в ультранизких концентрациях и специфические олигонуклеотиды для использования в способе
KR102493904B1 (ko) * 2019-12-13 2023-01-31 한국생명공학연구원 EeCpf1에 의해 IL2Rg 유전자가 돌연변이된 면역부전 동물모델 및 그 제조방법
RU2720768C1 (ru) * 2019-12-13 2020-05-13 Федеральное бюджетное учреждение науки "Центральный научно-исследовательский институт эпидемиологии" Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека (ФБУН ЦНИИ Эпидемиологии Роспотребнадзора) Система CRISPR-Cas для детекции провирусной ДНК вируса иммунодефицита человека, интегрированной в геном человека, в ультранизких концентрациях
US10704033B1 (en) 2019-12-13 2020-07-07 Inscripta, Inc. Nucleic acid-guided nucleases
RU2720769C1 (ru) * 2019-12-13 2020-05-13 Федеральное бюджетное учреждение науки «Центральный научно-исследовательский институт эпидемиологии» Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека (ФБУН ЦНИИ Эпидемиологии Роспотребнадзора) Способ получения препарата рибонуклеопротеинового комплекса CRISPR/CAS и препарат для детекции провирусной ДНК вируса иммунодефицита человека, интегрированной в геном человека, в ультранизких концентрациях
KR20220116485A (ko) 2019-12-16 2022-08-23 바스프 아그리컬쳐럴 솔루션즈 시드 유에스 엘엘씨 쌍을 형성한 닉카제를 이용하는 개선된 게놈 편집
US20230220405A1 (en) 2019-12-16 2023-07-13 BASF Agricultural Solutions Seed US LLC Precise introduction of dna or mutations into the genome of wheat
US11008557B1 (en) 2019-12-18 2021-05-18 Inscripta, Inc. Cascade/dCas3 complementation assays for in vivo detection of nucleic acid-guided nuclease edited cells
JP2023506284A (ja) 2019-12-19 2023-02-15 ビーエーエスエフ ソシエタス・ヨーロピア ファインケミカルを生成する際の空時収率、炭素変換効率、及び炭素基質適応性の増加
US20230041211A1 (en) 2019-12-20 2023-02-09 Basf Se Decreasing toxicity of terpenes and increasing the production potential in micro-organisms
WO2021138247A1 (en) 2019-12-30 2021-07-08 LifeEDIT Therapeutics, Inc. Rna-guided nucleases and active fragments and variants thereof and methods of use
CN111073902B (zh) * 2019-12-30 2022-04-26 广东省微生物研究所（广东省微生物分析检测中心） 提升胶霉毒素生物合成基因表达水平的CRISPR/dCas9载体及其构建方法和应用
CN113073118B (zh) * 2020-01-03 2023-09-15 珠海联邦制药股份有限公司 Tet-off表达调控系统在级联放大启动子活性中的用途
US10689669B1 (en) 2020-01-11 2020-06-23 Inscripta, Inc. Automated multi-module cell processing methods, instruments, and systems
US20230073514A1 (en) 2020-01-21 2023-03-09 Limagrain Europe Wheat haploid inducer plant and uses
WO2021151065A2 (en) * 2020-01-24 2021-07-29 The General Hospital Corporation Methods to characterize enzymes for genome engineering
WO2021154706A1 (en) 2020-01-27 2021-08-05 Inscripta, Inc. Electroporation modules and instrumentation
WO2021155109A1 (en) 2020-01-30 2021-08-05 Pairwise Plants Services, Inc. Compositions, systems, and methods for base diversification
CA3165291A1 (en) 2020-01-31 2021-08-05 Pairwise Plants Services, Inc. Suppression of shade avoidance response in plants
US20230063560A1 (en) 2020-02-04 2023-03-02 Pairwise Plans Services, Inc. Thornless and/or prickleless rubus plants
CN111337666B (zh) * 2020-02-12 2021-04-02 山东大学 I-motif重组介导的FRET探针及其原位成像癌细胞表面蛋白质同源二聚化的应用
CN113261532A (zh) * 2020-02-17 2021-08-17 中山大学 一种肺癌动物模型的构建方法
US20230357784A1 (en) 2020-02-21 2023-11-09 Limagrain Europe Prime editing technology for plant genome engineering
US20210261978A1 (en) 2020-02-21 2021-08-26 Pairwise Plants Services, Inc. Resistance to soybean cyst nematode through gene editing
JP2023515573A (ja) 2020-02-26 2023-04-13 ソレント・セラピューティクス・インコーポレイテッド 普遍的な遮蔽性部分を有する活性化可能な抗原結合性タンパク質
CA3169105A1 (en) 2020-02-28 2021-09-02 Ling MENG Method for rapid genome modification in recalcitrant plants
AU2020431316A1 (en) * 2020-02-28 2022-10-20 Huigene Therapeutics Co., Ltd. Type VI-E and type VI-F CRISPR-Cas system and uses thereof
EP4110929A1 (en) 2020-02-28 2023-01-04 KWS SAAT SE & Co. KGaA Immature inflorescence meristem editing
EP4114946A1 (en) 2020-03-04 2023-01-11 Regeneron Pharmaceuticals, Inc. Methods and compositions for sensitization of tumor cells to immune therapy
CA3174557A1 (en) * 2020-03-06 2021-09-10 Metagenomi, Inc. Class ii, type v crispr systems
EP3878958A1 (en) 2020-03-11 2021-09-15 B.R.A.I.N. Biotechnology Research And Information Network AG Crispr-cas nucleases from cpr-enriched metagenome
CA3173528A1 (en) 2020-03-11 2021-09-16 Omega Therapeutics, Inc. Compositions and methods for modulating forkhead box p3 (foxp3) gene expression
EP4125348A1 (en) 2020-03-23 2023-02-08 Regeneron Pharmaceuticals, Inc. Non-human animals comprising a humanized ttr locus comprising a v30m mutation and methods of use
US20210301282A1 (en) 2020-03-26 2021-09-30 Pairwise Plants Services, Inc. Methods for controlling meristem size for crop improvement
US11882808B2 (en) 2020-03-27 2024-01-30 Pairwise Plants Services, Inc. Methods for improving resistance to soybean rust
US20230263121A1 (en) 2020-03-31 2023-08-24 Elo Life Systems Modulation of endogenous mogroside pathway genes in watermelon and other cucurbits
WO2021207148A1 (en) 2020-04-06 2021-10-14 Pairwise Plants Services, Inc. Methods and compositions for increasing resistance to ear rot and stem rot disease in maize
WO2021204877A2 (en) 2020-04-08 2021-10-14 Astrazeneca Ab Compositions and methods for improved site-specific modification
RU2743861C1 (ru) * 2020-04-15 2021-03-01 Федеральное бюджетное учреждение науки "Центральный научно-исследовательский институт эпидемиологии" Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека (ФБУН ЦНИИ Эпидемиологии Роспотребнадзора) Система CRISPR-Cas для выявления гена антибиотикоустойчивости blaVIM-2 (металло-бета-лактамаза класс B VIM-2) Pseudomonas aeruginosa в ультранизких концентрациях
RU2745637C1 (ru) * 2020-04-15 2021-03-29 Федеральное бюджетное учреждение науки "Центральный научно-исследовательский институт эпидемиологии" Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека (ФБУН ЦНИИ Эпидемиологии Роспотребнадзора) Способ получения препарата рибонуклеопротеинового комплекса CRISPR/Cas и препарат для выявления гена антибиотикоустойчивости bla VIM-2 (металло-бета-лактамаза класс B VIM-2) Pseudomonas aeruginosa в ультранизких концентрациях
EP4135512A1 (en) 2020-04-16 2023-02-22 Pairwise Plants Services, Inc. Methods for controlling meristem size for crop improvement
US20210332388A1 (en) 2020-04-24 2021-10-28 Inscripta, Inc. Compositions, methods, modules and instruments for automated nucleic acid-guided nuclease editing in mammalian cells
EP4139345A1 (en) 2020-04-24 2023-03-01 Sorrento Therapeutics, Inc. Memory dimeric antigen receptors
TW202208626A (zh) 2020-04-24 2022-03-01 美商生命編輯公司 Rna引導核酸酶及其活性片段與變體，以及使用方法
EP4146794A1 (en) 2020-05-04 2023-03-15 Iovance Biotherapeutics, Inc. Processes for production of tumor infiltrating lymphocytes and uses of the same in immunotherapy
AU2021267940A1 (en) 2020-05-08 2022-12-08 President And Fellows Of Harvard College Methods and compositions for simultaneous editing of both strands of a target double-stranded nucleotide sequence
WO2021224506A1 (en) * 2020-05-08 2021-11-11 Universitat Pompeu Fabra Crispr-cas homology directed repair enhancer
WO2021231259A1 (en) 2020-05-11 2021-11-18 Precision Biosciences, Inc. Self-limiting viral vectors encoding nucleases
WO2021228699A1 (en) 2020-05-13 2021-11-18 Nunhems B.V. Tomato plants having suppressed meiotic recombination
CA3178193A1 (en) 2020-05-13 2021-11-18 Wim VRIEZEN Method for obtaining mutant plants by targeted mutagenesis
US11787841B2 (en) 2020-05-19 2023-10-17 Inscripta, Inc. Rationally-designed mutations to the thrA gene for enhanced lysine production in E. coli
WO2021243283A1 (en) * 2020-05-28 2021-12-02 University Of Southern California Scalable trio guide rna approach for integration of large donor dna
UY39237A (es) 2020-05-29 2021-12-31 Kws Saat Se & Co Kgaa Inducción de haploides en plantas
WO2021247477A1 (en) 2020-06-02 2021-12-09 Pairwise Plants Services, Inc. Methods for controlling meristem size for crop improvement
CA3178670A1 (en) * 2020-06-03 2021-12-09 Lucas Benjamin HARRINGTON Programmable nucleases and methods of use
CN111808832B (zh) * 2020-06-05 2022-02-11 华南农业大学 一种水稻纹枯病菌阳离子转运ATP酶基因及其片段Rscta和应用
JP7419168B2 (ja) 2020-06-10 2024-01-22 株式会社東芝 改変型ｐｉｇｇｙＢａｃトランスポゼースのポリペプチド、それをコードするポリヌクレオチド、導入キャリア、キット、細胞のゲノムに目的配列を組込む方法及び細胞製造方法
CA3186580A1 (en) 2020-06-12 2021-12-16 Sherlock Biosciences, Inc. Crispr-based sars-cov-2 detection
BR112022025598A2 (pt) 2020-06-17 2023-01-03 Pairwise Plants Services Inc Métodos para controlar o tamanho do meristema para melhoria da safra
EP4171616A1 (en) 2020-06-26 2023-05-03 Juno Therapeutics GmbH Engineered t cells conditionally expressing a recombinant receptor, related polynucleotides and methods
EP4172340A1 (en) 2020-06-29 2023-05-03 KWS SAAT SE & Co. KGaA Boosting homology directed repair in plants
EP4172328A1 (en) 2020-06-30 2023-05-03 Pairwise Plants Services, Inc. Compositions, systems, and methods for base diversification
AU2021310363A1 (en) 2020-07-15 2023-03-16 LifeEDIT Therapeutics, Inc. Uracil stabilizing proteins and active fragments and variants thereof and methods of use
CN111778252B (zh) * 2020-07-17 2022-01-07 湖北省农业科学院畜牧兽医研究所 一种靶向敲除SST基因的sgRNA及其CRISPR/Cas9系统和应用
EP3943600A1 (en) 2020-07-21 2022-01-26 B.R.A.I.N. Biotechnology Research And Information Network AG Novel, non-naturally occurring crispr-cas nucleases for genome editing
AU2021313163A1 (en) 2020-07-21 2023-02-16 Pairwise Plants Services, Inc. Optimized protein linkers and methods of use
EP4192875A1 (en) 2020-08-10 2023-06-14 Precision BioSciences, Inc. Antibodies and fragments specific for b-cell maturation antigen and uses thereof
US20230302134A1 (en) * 2020-08-13 2023-09-28 Yale University Compositions and methods for engineering and selection of car t cells with desired phenotypes
CN111979273B (zh) * 2020-08-24 2022-05-27 苏州启辰生物科技有限公司 一种制备人源化ace2小鼠模型的方法
MX2023002160A (es) 2020-08-28 2023-04-25 Pairwise Plants Services Inc Proteinas dise?adas y metodos de uso de las mismas.
WO2022051418A1 (en) 2020-09-01 2022-03-10 64-X, Inc. Mammalian cells and methods for engineering the same
EP4208544A2 (en) 2020-09-02 2023-07-12 Massachusetts Institute of Technology Systems, methods, and compositions for rna-guided rna-targeting crispr effectors
WO2022056254A2 (en) 2020-09-11 2022-03-17 LifeEDIT Therapeutics, Inc. Dna modifying enzymes and active fragments and variants thereof and methods of use
WO2022060749A1 (en) 2020-09-15 2022-03-24 Inscripta, Inc. Crispr editing to embed nucleic acid landing pads into genomes of live cells
CA3193089A1 (en) 2020-09-18 2022-03-24 Jamie KERSHNER Constructs and uses thereof for efficient and specific genome editing
WO2022188039A1 (en) 2021-03-09 2022-09-15 Huigene Therapeutics Co., Ltd. Engineered crispr/cas13 system and uses thereof
WO2022068912A1 (en) 2020-09-30 2022-04-07 Huigene Therapeutics Co., Ltd. Engineered crispr/cas13 system and uses thereof
WO2022076606A1 (en) 2020-10-06 2022-04-14 Iovance Biotherapeutics, Inc. Treatment of nsclc patients with tumor infiltrating lymphocyte therapies
JP2023546359A (ja) 2020-10-06 2023-11-02 アイオバンス バイオセラピューティクス，インコーポレイテッド 腫瘍浸潤リンパ球療法によるｎｓｃｌｃ患者の治療
US20230365995A1 (en) 2020-10-07 2023-11-16 Precision Biosciences, Inc. Lipid nanoparticle compositions
US20240060079A1 (en) 2020-10-23 2024-02-22 Elo Life Systems Methods for producing vanilla plants with improved flavor and agronomic production
EP4237568A1 (en) 2020-10-27 2023-09-06 KWS SAAT SE & Co. KGaA Use of enhanced pol theta activity for eukaryotic genome engineering
CN112300999A (zh) * 2020-11-02 2021-02-02 江苏集萃药康生物科技股份有限公司 一种balb/c小鼠模型及其用途
CA3200521A1 (en) 2020-11-06 2022-05-12 Pairwise Plants Services, Inc. Compositions and methods for rna-encoded dna-replacement of alleles
CN116406383A (zh) 2020-11-06 2023-07-07 日商基因编辑力股份有限公司 FokI核酸酶结构域的突变体
US11512297B2 (en) 2020-11-09 2022-11-29 Inscripta, Inc. Affinity tag for recombination protein recruitment
EP4243608A1 (en) 2020-11-11 2023-09-20 Leibniz-Institut für Pflanzenbiochemie (IPB) Fusion protein for editing endogenous dna of a eukaryotic cell
CN112410355B (zh) * 2020-11-23 2022-03-25 昆明理工大学 一种酰基辅酶a氧化酶2基因rkacox2及其应用
CN112461807B (zh) * 2020-11-26 2021-11-19 山西大学 碳量子点在靶向细胞核仁免洗成像中的应用
EP4256052A1 (en) 2020-12-02 2023-10-11 Decibel Therapeutics, Inc. Crispr sam biosensor cell lines and methods of use thereof
CN112195164B (zh) * 2020-12-07 2021-04-23 中国科学院动物研究所 工程化的Cas效应蛋白及其使用方法
CN114634928B (zh) * 2020-12-15 2023-08-04 四川大学华西医院 一种降低stat3转录功能的核酸片段及其制药用途
JP2023554651A (ja) * 2020-12-17 2023-12-28 モンサント テクノロジー エルエルシー 操作されたｓｓＤＮａｓｅ不含ＣＲＩＳＰＲエンドヌクレアーゼ
AU2021401302A1 (en) 2020-12-17 2023-07-06 Iovance Biotherapeutics, Inc. Treatment with tumor infiltrating lymphocyte therapies in combination with ctla-4 and pd-1 inhibitors
US20240123067A1 (en) 2020-12-17 2024-04-18 Iovance Biotherapeutics, Inc. Treatment of cancers with tumor infiltrating lymphocyte therapies
AU2021409952A1 (en) 2020-12-23 2023-07-06 Flagship Pioneering Innovations V, Inc. In vitro assembly of anellovirus capsids enclosing rna
CN112553215A (zh) * 2020-12-24 2021-03-26 山东农业大学 一个玉米纹枯病抗病相关基因ZmFAH49及其应用
CN112662670B (zh) * 2020-12-25 2024-04-02 河南省农业科学院 花生脂酰-酰基载体蛋白硫酯酶AhFATB2基因启动子及其制备方法和应用
CN112640847B (zh) * 2020-12-30 2022-12-13 重庆医科大学附属第一医院 一种内源性癫痫发作动物模型及其构建方法
CA3204158A1 (en) 2021-01-04 2022-07-07 Juhan Kim Mad nucleases
US11332742B1 (en) 2021-01-07 2022-05-17 Inscripta, Inc. Mad nucleases
JP2024502630A (ja) 2021-01-12 2024-01-22 マーチ セラピューティクス， インコーポレイテッド コンテキスト依存性二本鎖ｄｎａ特異的デアミナーゼ及びその使用
CN112680479A (zh) * 2021-01-13 2021-04-20 汪利平 Cyp1b1基因缺失斑马鱼的制备
CN114790456A (zh) * 2021-01-24 2022-07-26 东北林业大学 野菊u6基因及其应用
WO2022165111A1 (en) 2021-01-28 2022-08-04 Precision Biosciences, Inc. Modulation of tgf beta signaling in genetically-modified eukaryotic cells
TW202241508A (zh) 2021-01-29 2022-11-01 美商艾歐凡斯生物治療公司 細胞介素相關之腫瘤浸潤性淋巴球組合物及方法
CN112852745B (zh) * 2021-02-04 2023-05-23 中国农业科学院兰州兽医研究所 Hdac3基因敲除的bhk-21细胞系及其构建方法和应用
CN112877275B (zh) * 2021-02-04 2023-03-31 中国农业科学院兰州兽医研究所 Hdac2基因敲除的bhk-21细胞系及其构建方法和应用
CN116004572A (zh) * 2021-02-05 2023-04-25 山东舜丰生物科技有限公司 Crispr酶和系统以及应用
EP4288112A1 (en) * 2021-02-07 2023-12-13 Huidagene Therapeutics (Singapore) Pte. Ltd. Rna adeno-associated virus (raav) vector and uses thereof
EP4291678A1 (en) 2021-02-09 2023-12-20 Sherlock Biosciences, Inc. Nucleic acid amplification using promoter primers
BR112023015909A2 (pt) 2021-02-11 2023-11-21 Monsanto Technology Llc Métodos e composições para modificar níveis de citocinina oxidase em plantas
US11884924B2 (en) 2021-02-16 2024-01-30 Inscripta, Inc. Dual strand nucleic acid-guided nickase editing
WO2022175878A1 (en) * 2021-02-20 2022-08-25 Kcat Enzymatic Private Limited Insilico guided crispr-cas driven enzyme engineering framework
CA3211121A1 (en) 2021-02-25 2022-09-01 Pairwise Plants Services, Inc. Methods and compositions for modifying root architecture in plants
EP4301852A1 (en) 2021-03-02 2024-01-10 BRAIN Biotech AG Novel crispr-cas nucleases from metagenomes
JP2024509853A (ja) 2021-03-03 2024-03-05 ジュノー セラピューティクス インコーポレイテッド Ｔ細胞療法およびｄｇｋ阻害剤の組合せ
WO2022192391A1 (en) * 2021-03-09 2022-09-15 Arbor Biotechnologies, Inc. Compositions comprising a variant polypeptide and uses thereof
CN115427561A (zh) 2021-03-09 2022-12-02 辉大(上海)生物科技有限公司 工程化CRISPR/Cas13系统及其用途
CN113005147A (zh) * 2021-03-11 2021-06-22 中山大学附属第一医院 一种usp8突变的小鼠动物模型的构建方法及其应用
CA3212439A1 (en) 2021-03-19 2022-09-22 Michelle SIMPSON-ABELSON Methods for tumor infiltrating lymphocyte (til) expansion related to cd39/cd69 selection and gene knockout in tils
CA3173950A1 (en) 2021-03-22 2022-09-22 Tyson D. BOWEN Dna modifyng enzymes and active fragments and variants thereof and methods of use
JP2024513087A (ja) 2021-04-07 2024-03-21 アストラゼネカ・アクチエボラーグ 部位特異的改変のための組成物及び方法
WO2022217086A1 (en) 2021-04-09 2022-10-13 Vor Biopharma Inc. Photocleavable guide rnas and methods of use thereof
CA3215435A1 (en) 2021-04-16 2022-10-20 Giuseppe Ciaramella Genetic modification of hepatocytes
BR112023021665A2 (pt) 2021-04-19 2023-12-19 Iovance Biotherapeutics Inc Método para tratar um câncer, e, composição
KR102428773B1 (ko) * 2021-04-21 2022-08-05 (주)지플러스생명과학 분리형 crRNA 및 이를 이용한 CRISPR-Cas 시스템
WO2022226316A1 (en) 2021-04-22 2022-10-27 Precision Biosciences, Inc. Compositions and methods for generating male sterile plants
JP2024518413A (ja) 2021-05-06 2024-05-01 アーティザン ディヴェロップメント ラブス インコーポレイテッド 修飾ヌクレアーゼ
WO2022245754A1 (en) 2021-05-17 2022-11-24 Iovance Biotherapeutics, Inc. Pd-1 gene-edited tumor infiltrating lymphocytes and uses of same in immunotherapy
WO2022243437A1 (en) 2021-05-19 2022-11-24 KWS SAAT SE & Co. KGaA Sample preparation with oppositely oriented guide polynucleotides
CN113287569B (zh) * 2021-05-27 2023-04-07 四川康城生物科技有限公司 一种免疫低下动物模型的构建方法
US20220389418A1 (en) 2021-06-01 2022-12-08 Helmholtz-Institut Für Rna-Basierte Nfektionsforschung (Hzi) Rna-guided cas nucleases and uses thereof in diagnostics and therapy
CA3222023A1 (en) 2021-06-01 2022-12-08 Arbor Biotechnologies, Inc. Gene editing systems comprising a crispr nuclease and uses thereof
WO2022256448A2 (en) 2021-06-01 2022-12-08 Artisan Development Labs, Inc. Compositions and methods for targeting, editing, or modifying genes
CN113064220A (zh) * 2021-06-03 2021-07-02 四川九通智路科技有限公司 基于非线性自回归神经网络的能见度测量系统及测量方法
CA3173953A1 (en) 2021-06-11 2023-12-10 Tyson D. BOWEN Rna polymerase iii promoters and methods of use
US20220403475A1 (en) 2021-06-14 2022-12-22 Pairwise Plants Services, Inc. Reporter constructs, compositions comprising the same, and methods of use thereof
WO2022266271A1 (en) 2021-06-17 2022-12-22 Pairwise Plants Services, Inc. Modification of growth regulating factor family transcription factors in soybean
WO2022266439A2 (en) * 2021-06-17 2022-12-22 The Board Of Trustees Of The University Of Illinois Crispr cascade
WO2022266538A2 (en) 2021-06-18 2022-12-22 Artisan Development Labs, Inc. Compositions and methods for targeting, editing or modifying human genes
UY39827A (es) 2021-06-24 2023-01-31 Pairwise Plants Services Inc Modificación de genes de ubiquitina ligasa e3 hect para mejorar los rasgos de rendimiento
CN117794358A (zh) 2021-07-01 2024-03-29 成对植物服务股份有限公司 用于增强根系发育的方法和组合物
EP4368713A1 (en) * 2021-07-05 2024-05-15 Genkore Inc. Cleavage-inactive cas12f1, cleavage-inactive cas12f1-based fusion protein, crispr gene-editing system comprising same, and preparation method and use thereof
CN117987554A (zh) * 2021-07-06 2024-05-07 苏州淦江生物技术有限公司 一种基于Cas蛋白的检测方法
WO2023283585A2 (en) 2021-07-06 2023-01-12 Vor Biopharma Inc. Inhibitor oligonucleotides and methods of use thereof
EP4367242A2 (en) 2021-07-07 2024-05-15 Omega Therapeutics, Inc. Compositions and methods for modulating secreted frizzled receptor protein 1 (sfrp1) gene expression
CN113383747B (zh) * 2021-07-09 2022-12-27 广西贺州农贝贝农牧科技有限公司 一种产蛋后期老龄鸡强制换羽的工艺方法
WO2023283636A1 (en) * 2021-07-09 2023-01-12 Acrigen Biosciences Compositions and methods for nucleic acid modifications
WO2023287669A2 (en) 2021-07-12 2023-01-19 Labsimply, Inc. Nuclease cascade assay
WO2023004074A2 (en) 2021-07-22 2023-01-26 Iovance Biotherapeutics, Inc. Method for cryopreservation of solid tumor fragments
CA3226942A1 (en) 2021-07-28 2023-02-02 Iovance Biotherapeutics, Inc. Treatment of cancer patients with tumor infiltrating lymphocyte therapies in combination with kras inhibitors
CA3221517A1 (en) 2021-07-30 2023-02-02 Monika KLOIBER-MAITZ Plants with improved digestibility and marker haplotypes
AU2022324093A1 (en) 2021-08-02 2024-02-08 Vor Biopharma Inc. Compositions and methods for gene modification
CA3229056A1 (en) 2021-08-12 2023-02-16 Pairwise Plants Services, Inc. Modification of brassinosteroid receptor genes to improve yield traits
WO2023020938A1 (en) 2021-08-16 2023-02-23 Nunhems B.V. Lettuce plant having delayed bolting
CA3229224A1 (en) 2021-08-17 2023-02-23 Pairwise Plants Services, Inc. Methods and compositions for modifying cytokinin receptor histidine kinase genes in plants
WO2023028444A1 (en) * 2021-08-27 2023-03-02 Mammoth Biosciences, Inc. Effector proteins and methods of use
WO2023034731A1 (en) 2021-08-30 2023-03-09 Pairwise Plants Services, Inc. Modification of ubiquitin binding peptidase genes in plants for yield trait improvement
CN113584077B (zh) * 2021-08-31 2023-09-15 西南大学 沉默桑实杯盘菌关键致病基因CsGPA1的沉默载体及其应用和方法
AR126938A1 (es) 2021-09-02 2023-11-29 Pairwise Plants Services Inc Métodos y composiciones para mejorar la arquitectura de las plantas y los rasgos de rendimiento
US20230266293A1 (en) 2021-09-21 2023-08-24 Pairwise Plants Services, Inc. Color-based and/or visual methods for identifying the presence of a transgene and compositions and constructs relating to the same
WO2023049720A1 (en) 2021-09-21 2023-03-30 Pairwise Plants Services, Inc. Methods and compositions for reducing pod shatter in canola
WO2023049926A2 (en) 2021-09-27 2023-03-30 Vor Biopharma Inc. Fusion polypeptides for genetic editing and methods of use thereof
WO2023052508A2 (en) 2021-09-30 2023-04-06 Astrazeneca Ab Use of inhibitors to increase efficiency of crispr/cas insertions
US20230108968A1 (en) 2021-10-04 2023-04-06 Pairwise Plants Services, Inc. Methods for improving floret fertility and seed yield
CA3234455A1 (en) 2021-10-07 2023-04-13 Pairwise Plants Services, Inc. Methods for improving floret fertility and seed yield
WO2023064872A1 (en) 2021-10-14 2023-04-20 Precision Biosciences, Inc. Combinations of anti-bcma car t cells and gamma secretase inhibitors
EP4166670A1 (en) 2021-10-18 2023-04-19 KWS SAAT SE & Co. KGaA Plant-tag-based weeding control
WO2023070003A1 (en) 2021-10-19 2023-04-27 Precision Biosciences, Inc. Gene editing methods for treating alpha-1 antitrypsin (aat) deficiency
CA3235824A1 (en) 2021-10-27 2023-05-04 Frederick G. Vogt Systems and methods for coordinating manufacturing of cells for patient-specific immunotherapy
WO2023077053A2 (en) 2021-10-28 2023-05-04 Regeneron Pharmaceuticals, Inc. Crispr/cas-related methods and compositions for knocking out c5
WO2023077148A1 (en) 2021-11-01 2023-05-04 Tome Biosciences, Inc. Single construct platform for simultaneous delivery of gene editing machinery and nucleic acid cargo
WO2023081900A1 (en) 2021-11-08 2023-05-11 Juno Therapeutics, Inc. Engineered t cells expressing a recombinant t cell receptor (tcr) and related systems and methods
WO2023086803A1 (en) 2021-11-10 2023-05-19 Iovance Biotherapeutics, Inc. Methods of expansion treatment utilizing cd8 tumor infiltrating lymphocytes
WO2023091910A1 (en) 2021-11-16 2023-05-25 Precision Biosciences, Inc. Methods for cancer immunotherapy
WO2023097316A1 (en) * 2021-11-29 2023-06-01 Editas Medicine, Inc. Engineered crispr/cas12a effector proteins, and uses thereof
CN114404429B (zh) * 2021-11-30 2023-06-30 重庆医科大学附属第二医院 一种纳米银修饰的单宁酸-铁网络的载药纳米复合物及其制备方法和逆转肿瘤耐药应用
WO2023108047A1 (en) 2021-12-08 2023-06-15 Regeneron Pharmaceuticals, Inc. Mutant myocilin disease model and uses thereof
AR127904A1 (es) 2021-12-09 2024-03-06 Pairwise Plants Services Inc Métodos para mejorar la fertilidad de floretes y el rendimiento de semillas
WO2023114090A2 (en) * 2021-12-13 2023-06-22 Labsimply, Inc. Signal boost cascade assay
US11820983B2 (en) 2021-12-13 2023-11-21 Labsimply, Inc. Tuning cascade assay kinetics via molecular design
WO2023114750A1 (en) 2021-12-13 2023-06-22 Pairwise Plants Services, Inc. Model editing systems and methods relating to the same
WO2023119135A1 (en) 2021-12-21 2023-06-29 Benson Hill, Inc. Compositions and methods for modifying genomes
WO2023122764A1 (en) 2021-12-22 2023-06-29 Tome Biosciences, Inc. Co-delivery of a gene editor construct and a donor template
WO2023122800A1 (en) 2021-12-23 2023-06-29 University Of Massachusetts Therapeutic treatment for fragile x-associated disorder
WO2023133440A1 (en) 2022-01-06 2023-07-13 Pairwise Plants Services, Inc. Methods and compositions for trichome removal
WO2023135524A1 (en) * 2022-01-12 2023-07-20 Genecker Co., Ltd. Cas9 proteins with enhanced specificity and uses thereof
WO2023139557A1 (en) 2022-01-24 2023-07-27 LifeEDIT Therapeutics, Inc. Rna-guided nucleases and active fragments and variants thereof and methods of use
WO2023147488A1 (en) 2022-01-28 2023-08-03 Iovance Biotherapeutics, Inc. Cytokine associated tumor infiltrating lymphocytes compositions and methods
AR128372A1 (es) 2022-01-31 2024-04-24 Pairwise Plants Services Inc Supresión de la respuesta de evitación de la sombra en las plantas
WO2023150623A2 (en) 2022-02-02 2023-08-10 Regeneron Pharmaceuticals, Inc. Anti-tfr:gaa and anti-cd63:gaa insertion for treatment of pompe disease
WO2023154887A1 (en) 2022-02-11 2023-08-17 Northeast Agricultural University Methods and compositions for increasing protein and/or oil content and modifying oil profile in a plant
WO2023156585A1 (en) * 2022-02-18 2023-08-24 Pulmobiotics, S.L. Systems, tools, and methods for engineering bacteria
US20230383271A1 (en) 2022-02-28 2023-11-30 Pairwise Plants Services, Inc. Engineered proteins and methods of use thereof
WO2023167882A1 (en) 2022-03-01 2023-09-07 Artisan Development Labs, Inc. Composition and methods for transgene insertion
WO2023168217A1 (en) 2022-03-02 2023-09-07 Pairwise Plants Services, Inc. Modification of brassinosteroid receptor genes to improve yield traits
CN114271237B (zh) * 2022-03-07 2022-05-27 南京农业大学 一种长短绒自别雌雄鸽的培育方法
WO2023192838A1 (en) 2022-03-31 2023-10-05 Pairwise Plants Services, Inc. Early flowering rosaceae plants with improved characteristics
CN114592023B (zh) * 2022-03-31 2023-03-24 杭州优玛达生物科技有限公司 一种细胞裂解自组装多肽复合物、自组装方法、自组装多肽制剂及应用
WO2023196877A1 (en) 2022-04-06 2023-10-12 Iovance Biotherapeutics, Inc. Treatment of nsclc patients with tumor infiltrating lymphocyte therapies
US20230357789A1 (en) 2022-04-07 2023-11-09 Pairwise Plants Services, Inc. Methods and compositions for improving resistance to fusarium head blight
WO2023201369A1 (en) 2022-04-15 2023-10-19 Iovance Biotherapeutics, Inc. Til expansion processes using specific cytokine combinations and/or akti treatment
WO2023205744A1 (en) 2022-04-20 2023-10-26 Tome Biosciences, Inc. Programmable gene insertion compositions
US20230383305A1 (en) 2022-04-21 2023-11-30 Pairwise Plants Services, Inc. Methods and compositions for improving yield traits
WO2023212677A2 (en) 2022-04-29 2023-11-02 Regeneron Pharmaceuticals, Inc. Identification of tissue-specific extragenic safe harbors for gene therapy approaches
US20230348922A1 (en) 2022-05-02 2023-11-02 Pairwise Plants Services, Inc. Methods and compositions for enhancing yield and disease resistance
WO2023215831A1 (en) 2022-05-04 2023-11-09 Tome Biosciences, Inc. Guide rna compositions for programmable gene insertion
WO2023215809A1 (en) 2022-05-05 2023-11-09 Pairwise Plants Services, Inc. Methods and compositions for modifying root architecture and/or improving plant yield traits
WO2023220603A1 (en) 2022-05-09 2023-11-16 Regeneron Pharmaceuticals, Inc. Vectors and methods for in vivo antibody production
WO2023220608A1 (en) 2022-05-10 2023-11-16 Iovance Biotherapeutics, Inc. Treatment of cancer patients with tumor infiltrating lymphocyte therapies in combination with an il-15r agonist
WO2023225410A2 (en) 2022-05-20 2023-11-23 Artisan Development Labs, Inc. Systems and methods for assessing risk of genome editing events
WO2023225670A2 (en) 2022-05-20 2023-11-23 Tome Biosciences, Inc. Ex vivo programmable gene insertion
WO2023230498A1 (en) 2022-05-25 2023-11-30 Massachusetts Institute Of Technology Systems, methods, and compositions for rna-guided rna-targeting crispr effectors with cas7-11 variants
CN114934031B (zh) * 2022-05-25 2023-08-01 广州瑞风生物科技有限公司 新型Cas效应蛋白、基因编辑系统及用途
WO2023230566A2 (en) 2022-05-25 2023-11-30 Flagship Pioneering Innovations Vii, Llc Compositions and methods for modulating cytokines
WO2023230570A2 (en) 2022-05-25 2023-11-30 Flagship Pioneering Innovations Vii, Llc Compositions and methods for modulating genetic drivers
WO2023230573A2 (en) 2022-05-25 2023-11-30 Flagship Pioneering Innovations Vii, Llc Compositions and methods for modulation of immune responses
WO2023230578A2 (en) 2022-05-25 2023-11-30 Flagship Pioneering Innovations Vii, Llc Compositions and methods for modulating circulating factors
WO2023230549A2 (en) 2022-05-25 2023-11-30 Flagship Pioneering Innovations Vii, Llc Compositions and methods for modulation of tumor suppressors and oncogenes
WO2023235725A2 (en) 2022-05-31 2023-12-07 Regeneron Pharmaceuticals, Inc. Crispr-based therapeutics for c9orf72 repeat expansion disease
WO2023235726A2 (en) 2022-05-31 2023-12-07 Regeneron Pharmaceuticals, Inc. Crispr interference therapeutics for c9orf72 repeat expansion disease
WO2024006679A1 (en) 2022-06-27 2024-01-04 Pairwise Plants Services, Inc. Methods and compositions for modifying shade avoidance in plants
US20240000031A1 (en) 2022-06-29 2024-01-04 Pairwise Plants Services, Inc. Methods and compositions for controlling meristem size for crop improvement
WO2024006792A1 (en) 2022-06-29 2024-01-04 Pairwise Plants Services, Inc. Methods and compositions for controlling meristem size for crop improvement
EP4299733A1 (en) 2022-06-30 2024-01-03 Inari Agriculture Technology, Inc. Compositions, systems, and methods for genome editing
WO2024005864A1 (en) 2022-06-30 2024-01-04 Inari Agriculture Technology, Inc. Compositions, systems, and methods for genome editing
EP4299739A1 (en) 2022-06-30 2024-01-03 Inari Agriculture Technology, Inc. Compositions, systems, and methods for genome editing
WO2024005863A1 (en) 2022-06-30 2024-01-04 Inari Agriculture Technology, Inc. Compositions, systems, and methods for genome editing
WO2024020587A2 (en) 2022-07-22 2024-01-25 Tome Biosciences, Inc. Pleiopluripotent stem cell programmable gene insertion
WO2024026474A1 (en) 2022-07-29 2024-02-01 Regeneron Pharmaceuticals, Inc. Compositions and methods for transferrin receptor (tfr)-mediated delivery to the brain and muscle
WO2024030984A1 (en) 2022-08-04 2024-02-08 Pairwise Plants Services, Inc. Methods and compositions for improving yield traits
WO2024036240A1 (en) 2022-08-11 2024-02-15 Pairwise Plants Services, Inc. Methods and compositions for controlling meristem size for crop improvement
WO2024033901A1 (en) 2022-08-12 2024-02-15 LifeEDIT Therapeutics, Inc. Rna-guided nucleases and active fragments and variants thereof and methods of use
WO2024038168A1 (en) 2022-08-19 2024-02-22 UCB Biopharma SRL Novel rna-guided nucleases and nucleic acid targeting systems comprising such
WO2024042489A1 (en) 2022-08-25 2024-02-29 LifeEDIT Therapeutics, Inc. Chemical modification of guide rnas with locked nucleic acid for rna guided nuclease-mediated gene editing
US20240100192A1 (en) 2022-08-25 2024-03-28 Massachusetts Institute Of Technology Programmable rna writing using crispr effectors and trans-splicing templates
WO2024042165A2 (en) 2022-08-26 2024-02-29 UCB Biopharma SRL Novel rna-guided nucleases and nucleic acid targeting systems comprising such rna-guided nucleases
WO2024042168A1 (en) 2022-08-26 2024-02-29 UCB Biopharma SRL Novel rna-guided nucleases and nucleic acid targeting systems comprising such rna-guided nucleases
WO2024042199A1 (en) 2022-08-26 2024-02-29 KWS SAAT SE & Co. KGaA Use of paired genes in hybrid breeding
WO2024050548A2 (en) * 2022-09-02 2024-03-07 Hunterian Medicine Llc Compact promoters for targeting hypoxia induced genes
WO2024047562A1 (en) 2022-09-02 2024-03-07 Janssen Biotech, Inc. Materials and processes for bioengineering cellular hypoimmunogenicity
US20240090466A1 (en) 2022-09-08 2024-03-21 Pairwise Plants Services, Inc. Methods and compositions for improving yield characteristics in plants
WO2024054944A1 (en) 2022-09-08 2024-03-14 Juno Therapeutics, Inc. Combination of a t cell therapy and continuous or intermittent dgk inhibitor dosing
WO2024062138A1 (en) 2022-09-23 2024-03-28 Mnemo Therapeutics Immune cells comprising a modified suv39h1 gene
WO2024073606A1 (en) 2022-09-28 2024-04-04 Regeneron Pharmaceuticals, Inc. Antibody resistant modified receptors to enhance cell-based therapies
WO2024073751A1 (en) 2022-09-29 2024-04-04 Vor Biopharma Inc. Methods and compositions for gene modification and enrichment
WO2024076473A1 (en) * 2022-10-02 2024-04-11 Vedabio, Inc. Dimerization screening assays
WO2024083579A1 (en) 2022-10-20 2024-04-25 Basf Se Regulatory nucleic acid molecules for enhancing gene expression in plants
CN116355878B (zh) * 2023-02-28 2024-04-26 华中农业大学 新型TnpB编程性核酸酶及其应用
CN116751763B (zh) * 2023-05-08 2024-02-13 珠海舒桐医疗科技有限公司 一种Cpf1蛋白、V型基因编辑系统及应用
CN116671491B (zh) * 2023-07-04 2024-01-23 四川省畜牧科学研究院 一种南江黄羊的种羊选育方法
CN116790559B (zh) * 2023-07-07 2023-11-28 山东舜丰生物科技有限公司 一种融合HNH结构域的V型Cas酶及其应用
CN117286039B (zh) * 2023-11-27 2024-03-12 中国科学院天津工业生物技术研究所 一种菌丝形态优化的里氏木霉菌株及其应用

Family Cites Families (150)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1506509A (en) 1975-12-19 1978-04-05 Nissan Motor Apparatus for mounting instruments on the instrument panel of a motor vehicle
US4217344A (en) 1976-06-23 1980-08-12 L'oreal Compositions containing aqueous dispersions of lipid spheres
US4235871A (en) 1978-02-24 1980-11-25 Papahadjopoulos Demetrios P Method of encapsulating biologically active materials in lipid vesicles
US4186183A (en) 1978-03-29 1980-01-29 The United States Of America As Represented By The Secretary Of The Army Liposome carriers in chemotherapy of leishmaniasis
US4261975A (en) 1979-09-19 1981-04-14 Merck & Co., Inc. Viral liposome particle
US4485054A (en) 1982-10-04 1984-11-27 Lipoderm Pharmaceuticals Limited Method of encapsulating biologically active materials in multilamellar lipid vesicles (MLV)
US4501728A (en) 1983-01-06 1985-02-26 Technology Unlimited, Inc. Masking of liposomes from RES recognition
US5049386A (en) 1985-01-07 1991-09-17 Syntex (U.S.A.) Inc. N-ω,(ω-1)-dialkyloxy)- and N-(ω,(ω-1)-dialkenyloxy)Alk-1-YL-N,N,N-tetrasubstituted ammonium lipids and uses therefor
US4897355A (en) 1985-01-07 1990-01-30 Syntex (U.S.A.) Inc. N[ω,(ω-1)-dialkyloxy]- and N-[ω,(ω-1)-dialkenyloxy]-alk-1-yl-N,N,N-tetrasubstituted ammonium lipids and uses therefor
US4946787A (en) 1985-01-07 1990-08-07 Syntex (U.S.A.) Inc. N-(ω,(ω-1)-dialkyloxy)- and N-(ω,(ω-1)-dialkenyloxy)-alk-1-yl-N,N,N-tetrasubstituted ammonium lipids and uses therefor
US4797368A (en) 1985-03-15 1989-01-10 The United States Of America As Represented By The Department Of Health And Human Services Adeno-associated virus as eukaryotic expression vector
US4774085A (en) 1985-07-09 1988-09-27 501 Board of Regents, Univ. of Texas Pharmaceutical administration systems containing a mixture of immunomodulators
DE122007000007I2 (de) 1986-04-09 2010-12-30 Genzyme Corp Genetisch transformierte Tiere, die ein gewünschtes Protein in Milch absondern
US4837028A (en) 1986-12-24 1989-06-06 Liposome Technology, Inc. Liposomes with enhanced circulation time
US4873316A (en) 1987-06-23 1989-10-10 Biogen, Inc. Isolation of exogenous recombinant proteins from the milk of transgenic mammals
US5703055A (en) 1989-03-21 1997-12-30 Wisconsin Alumni Research Foundation Generation of antibodies through lipid mediated DNA delivery
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5264618A (en) 1990-04-19 1993-11-23 Vical, Inc. Cationic lipids for intracellular delivery of biologically active molecules
WO1991017424A1 (en) 1990-05-03 1991-11-14 Vical, Inc. Intracellular delivery of biologically active substances by means of self-assembling lipid complexes
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5266317A (en) 1990-10-04 1993-11-30 University Of Georgia Research Foundation, Inc. Insect-specific paralytic neurotoxin genes for use in biological insect control: methods and compositions
US5173414A (en) 1990-10-30 1992-12-22 Applied Immune Sciences, Inc. Production of recombinant adeno-associated virus vectors
GB9104617D0 (en) 1991-03-05 1991-04-17 Nickerson Int Seed Pest control
GB9114259D0 (en) 1991-07-02 1991-08-21 Ici Plc Plant derived enzyme and dna sequences
GB9115909D0 (en) 1991-07-23 1991-09-04 Nickerson Int Seed Recombinant dna
DK39692D0 (da) 1992-03-25 1992-03-25 Danisco Biologisk materiale
US5587308A (en) 1992-06-02 1996-12-24 The United States Of America As Represented By The Department Of Health & Human Services Modified adeno-associated virus vector capable of expression from a novel promoter
BR9306802A (pt) 1992-07-27 1998-12-08 Pioneer Hi Bred Int Processo independente de genótipos para produção de planta de soja transgénica e processo de regeneração de plantas de soja a partir de nodos cotiledonais
US5593972A (en) 1993-01-26 1997-01-14 The Wistar Institute Genetic immunization
US5814618A (en) 1993-06-14 1998-09-29 Basf Aktiengesellschaft Methods for regulating gene expression
US5789156A (en) 1993-06-14 1998-08-04 Basf Ag Tetracycline-regulated transcriptional inhibitors
US5543158A (en) 1993-07-23 1996-08-06 Massachusetts Institute Of Technology Biodegradable injectable nanoparticles
US6007845A (en) 1994-07-22 1999-12-28 Massachusetts Institute Of Technology Nanoparticles and microparticles of non-linear hydrophilic-hydrophobic multiblock copolymers
US5994627A (en) 1995-03-31 1999-11-30 Common Wealth Scientific And Industrial Research Organisation Genetic sequences conferring nematode resistance in plants and uses therefor
WO1996039154A1 (en) 1995-06-06 1996-12-12 Isis Pharmaceuticals, Inc. Oligonucleotides having phosphorothioate linkages of high chiral purity
US5985662A (en) 1995-07-13 1999-11-16 Isis Pharmaceuticals Inc. Antisense inhibition of hepatitis B virus replication
AR006928A1 (es) 1996-05-01 1999-09-29 Pioneer Hi Bred Int Una molecula de adn aislada que codifica una proteina fluorescente verde como marcador rastreable para la transformacion de plantas, un metodo para laproduccion de plantas transgenicas, un vector de expresion, una planta transgenica y celulas de dichas plantas.
US5985309A (en) 1996-05-24 1999-11-16 Massachusetts Institute Of Technology Preparation of particles for inhalation
US5855913A (en) 1997-01-16 1999-01-05 Massachusetts Instite Of Technology Particles incorporating surfactants for pulmonary drug delivery
US5846946A (en) 1996-06-14 1998-12-08 Pasteur Merieux Serums Et Vaccins Compositions and methods for administering Borrelia DNA
GB9907461D0 (en) 1999-03-31 1999-05-26 King S College London Neurite regeneration
US7022896B1 (en) 1997-04-04 2006-04-04 Board Of Regents Of University Of Nebraska Methods and materials for making and using transgenic dicamba-degrading organisms
GB9720465D0 (en) 1997-09-25 1997-11-26 Oxford Biomedica Ltd Dual-virus vectors
CA2307016A1 (en) 1997-10-24 1999-05-06 Life Technologies, Inc. Recombinational cloning using nucleic acids having recombination sites
US6750059B1 (en) 1998-07-16 2004-06-15 Whatman, Inc. Archiving of vectors
US6534261B1 (en) 1999-01-12 2003-03-18 Sangamo Biosciences, Inc. Regulation of endogenous gene expression in cells using zinc finger proteins
US7868149B2 (en) 1999-07-20 2011-01-11 Monsanto Technology Llc Plant genome sequence and uses thereof
US6603061B1 (en) 1999-07-29 2003-08-05 Monsanto Company Agrobacterium-mediated plant transformation method
GB0024550D0 (da) 2000-10-06 2000-11-22 Oxford Biomedica Ltd
WO2003045304A2 (en) 2001-09-26 2003-06-05 Mayo Foundation For Medical Education And Research Mutable vaccines
GB0125216D0 (en) 2001-10-19 2001-12-12 Univ Strathclyde Dendrimers for use in targeted delivery
US20090100536A1 (en) 2001-12-04 2009-04-16 Monsanto Company Transgenic plants with enhanced agronomic traits
JP2005512598A (ja) 2001-12-21 2005-05-12 オックスフォード バイオメディカ （ユーケー） リミテッド Ｅｉａｖなどのレンチウイルス発現ベクターを使用するトランスジェニック生物の作製方法
ATE485031T1 (de) 2002-06-28 2010-11-15 Protiva Biotherapeutics Inc Verfahren und vorrichtung zur herstellung von liposomen
GB0220467D0 (en) 2002-09-03 2002-10-09 Oxford Biomedica Ltd Composition
US20040142476A1 (en) 2002-11-01 2004-07-22 New England Biolabs, Inc. Organellar targeting of RNA and its use in the interruption of environmental gene flow
EP2567693B1 (en) 2003-07-16 2015-10-21 Protiva Biotherapeutics Inc. Lipid encapsulated interfering RNA
NZ592917A (en) 2003-09-15 2012-12-21 Protiva Biotherapeutics Inc Stable polyethyleneglycol (PEG) dialkyloxypropyl (DAA) lipid conjugates
GB0325379D0 (en) 2003-10-30 2003-12-03 Oxford Biomedica Ltd Vectors
US7919277B2 (en) * 2004-04-28 2011-04-05 Danisco A/S Detection and typing of bacterial strains
US7745651B2 (en) 2004-06-07 2010-06-29 Protiva Biotherapeutics, Inc. Cationic lipids and methods of use
US7799565B2 (en) 2004-06-07 2010-09-21 Protiva Biotherapeutics, Inc. Lipid encapsulated interfering RNA
JP4772045B2 (ja) 2004-07-16 2011-09-14 アメリカ合衆国 Ｃｍｖ／ｒ核酸コンストラクトを含むａｉｄｓに対するワクチン
GB0422877D0 (en) 2004-10-14 2004-11-17 Univ Glasgow Bioactive polymers
WO2007048046A2 (en) 2005-10-20 2007-04-26 Protiva Biotherapeutics, Inc. Sirna silencing of filovirus gene expression
EP1950294B1 (en) 2005-10-28 2012-01-18 Mitsubishi Tanabe Pharma Corporation Novel cell penetrating peptide
US8101741B2 (en) 2005-11-02 2012-01-24 Protiva Biotherapeutics, Inc. Modified siRNA molecules and uses thereof
GB0526211D0 (en) 2005-12-22 2006-02-01 Oxford Biomedica Ltd Viral vectors
US7915399B2 (en) 2006-06-09 2011-03-29 Protiva Biotherapeutics, Inc. Modified siRNA molecules and uses thereof
JP2008078613A (ja) 2006-08-24 2008-04-03 Rohm Co Ltd 窒化物半導体の製造方法及び窒化物半導体素子
US20100099858A1 (en) 2006-09-28 2010-04-22 Mirkin Chad A Maximizing Oligonucleotide Loading on Gold Nanoparticle
US8642295B2 (en) 2007-01-11 2014-02-04 Erasmus University Medical Center Circular chromosome conformation capture (4C)
US7838729B2 (en) 2007-02-26 2010-11-23 Monsanto Technology Llc Chloroplast transit peptides for efficient targeting of DMO and uses thereof
WO2008149176A1 (en) 2007-06-06 2008-12-11 Cellectis Meganuclease variants cleaving a dna target sequence from the mouse rosa26 locus and uses thereof
US8349554B2 (en) 2007-08-14 2013-01-08 Fred Hutchinson Cancer Research Center Needle array assembly and method for delivering therapeutic agents
WO2009082607A2 (en) 2007-12-04 2009-07-02 Alnylam Pharmaceuticals, Inc. Targeting lipids
CN101970051A (zh) 2007-12-31 2011-02-09 纳诺科尔治疗公司 用于治疗心力衰竭的rna干扰
WO2009127060A1 (en) 2008-04-15 2009-10-22 Protiva Biotherapeutics, Inc. Novel lipid formulations for nucleic acid delivery
FR2930264B1 (fr) * 2008-04-18 2013-02-22 Gervais Danone Sa Nouvelle souche de lactobacillus paracasei subsp. paracasei dotee de proprietes antimicrobiennes et immunomodulatrices.
JP2011523557A (ja) 2008-06-04 2011-08-18 メディカル リサーチ カウンシル ペプチド
US8999945B2 (en) 2008-06-30 2015-04-07 Silenseed Ltd Methods, compositions and systems for local delivery of drugs
WO2010004594A1 (en) 2008-07-08 2010-01-14 S.I.F.I. Societa' Industria Farmaceutica Italiana S.P.A. Ophthalmic compositions for treating pathologies of the posterior segment of the eye
US20100076057A1 (en) 2008-09-23 2010-03-25 Northwestern University TARGET DNA INTERFERENCE WITH crRNA
MX2011004859A (es) 2008-11-07 2011-08-03 Massachusetts Inst Technology Lipidoides de aminoalcohol y usos de los mismos.
GB2465749B (en) 2008-11-25 2013-05-08 Algentech Sas Plant cell transformation method
EP2427577A4 (en) 2009-05-04 2013-10-23 Hutchinson Fred Cancer Res PSEUDOTYPED RETROVIRAL VECTORS WITH COCAL VESICULOVIRUS SHELL
CA2767127A1 (en) 2009-07-01 2011-01-06 Protiva Biotherapeutics, Inc. Novel lipid formulations for delivery of therapeutic agents to solid tumors
CA2767129C (en) 2009-07-01 2015-01-06 Protiva Biotherapeutics, Inc. Compositions and methods for silencing apolipoprotein b
US8927807B2 (en) 2009-09-03 2015-01-06 The Regents Of The University Of California Nitrate-responsive promoter
US8889394B2 (en) 2009-09-07 2014-11-18 Empire Technology Development Llc Multiple domain proteins
SG181904A1 (en) 2009-12-23 2012-07-30 Novartis Ag Lipids, lipid compositions, and methods of using them
MX2012009178A (es) 2010-02-24 2012-11-30 Arrowhead Res Corp Composiciones para liberacion dirigida de arnsi.
CA2796600C (en) 2010-04-26 2019-08-13 Sangamo Biosciences, Inc. Genome editing of a rosa locus using zinc-finger nucleases
US8372951B2 (en) 2010-05-14 2013-02-12 National Tsing Hua University Cell penetrating peptides for intracellular delivery
CN102946907A (zh) 2010-05-28 2013-02-27 牛津生物医学（英国）有限公司 慢病毒载体向脑的递送
US9193827B2 (en) 2010-08-26 2015-11-24 Massachusetts Institute Of Technology Poly(beta-amino alcohols), their preparation, and uses thereof
EP2630156B1 (en) * 2010-10-20 2018-08-22 DuPont Nutrition Biosciences ApS Lactococcus crispr-cas sequences
US9405700B2 (en) 2010-11-04 2016-08-02 Sonics, Inc. Methods and apparatus for virtualization in an integrated circuit
EP2691443B1 (en) 2011-03-28 2021-02-17 Massachusetts Institute of Technology Conjugated lipomers and uses thereof
AU2012236099A1 (en) 2011-03-31 2013-10-03 Moderna Therapeutics, Inc. Delivery and formulation of engineered nucleic acids
US20120295960A1 (en) 2011-05-20 2012-11-22 Oxford Biomedica (Uk) Ltd. Treatment regimen for parkinson's disease
EP2760286B1 (en) 2011-09-26 2016-09-07 Sumitomo Chemical Company Limited Plant disease control composition and its use
EP2791160B1 (en) 2011-12-16 2022-03-02 ModernaTX, Inc. Modified mrna compositions
US20130185823A1 (en) 2012-01-16 2013-07-18 Academia Sinica Mesoporous silica nanoparticle-mediated delivery of dna into arabidopsis root
MX2014001965A (es) 2012-04-18 2014-03-31 Arrowhead Res Corp Polimeros de poli(acrilato) para suministro de acido nucleico in vivo.
LT3401400T (lt) 2012-05-25 2019-06-10 The Regents Of The University Of California Būdai ir kompozicijos, skirtos rnr molekulės nukreipiamai tikslinės dnr modifikacijai ir rnr molekulės nukreipiamam transkripcijos moduliavimui
US8614194B1 (en) 2012-07-25 2013-12-24 Kaohsiung Medical University Anionic cell penetrating peptide and its use for intracellular delivery
PT3494997T (pt) 2012-07-25 2019-12-05 Massachusetts Inst Technology Proteínas de ligação a adn indutíveis e ferramentas de perturbação do genoma e aplicações destas
EP4234696A3 (en) 2012-12-12 2023-09-06 The Broad Institute Inc. Crispr-cas component systems, methods and compositions for sequence manipulation
PT2921557T (pt) 2012-12-12 2016-10-19 Massachusetts Inst Technology Engenharia de sistemas, métodos e composições guia otimizadas para a manipulação de sequências
WO2014093694A1 (en) * 2012-12-12 2014-06-19 The Broad Institute, Inc. Crispr-cas nickase systems, methods and compositions for sequence manipulation in eukaryotes
DK2898075T3 (da) 2012-12-12 2016-06-27 Broad Inst Inc Konstruktion og optimering af forbedrede systemer, fremgangsmåder og enzymsammensætninger til sekvensmanipulation
ES2658401T3 (es) 2012-12-12 2018-03-09 The Broad Institute, Inc. Suministro, modificación y optimización de sistemas, métodos y composiciones para la manipulación de secuencias y aplicaciones terapéuticas
US8697359B1 (en) * 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products
EP2931899A1 (en) 2012-12-12 2015-10-21 The Broad Institute, Inc. Functional genomics using crispr-cas systems, compositions, methods, knock out libraries and applications thereof
CA3081054A1 (en) 2012-12-17 2014-06-26 President And Fellows Of Harvard College Rna-guided human genome engineering
WO2014118272A1 (en) 2013-01-30 2014-08-07 Santaris Pharma A/S Antimir-122 oligonucleotide carbohydrate conjugates
KR101780885B1 (ko) 2013-03-14 2017-10-11 카리부 바이오사이언시스 인코포레이티드 핵산-표적화 핵산의 조성물 및 방법
JP6670743B2 (ja) 2013-05-29 2020-03-25 セレクティスＣｅｌｌｅｃｔｉｓ Ｉｉ型ｃｒｉｓｐｒシステムにおける新規のコンパクトなｃａｓ９足場
US20140356956A1 (en) * 2013-06-04 2014-12-04 President And Fellows Of Harvard College RNA-Guided Transcriptional Regulation
AU2014274939B2 (en) * 2013-06-04 2020-03-19 President And Fellows Of Harvard College RNA-guideded transcriptional regulation
EP3417880A1 (en) 2013-06-05 2018-12-26 Duke University Rna-guided gene editing and gene regulation
WO2014204725A1 (en) 2013-06-17 2014-12-24 The Broad Institute Inc. Optimized crispr-cas double nickase systems, methods and compositions for sequence manipulation
ES2777217T3 (es) 2013-06-17 2020-08-04 Broad Inst Inc Suministro, modificación y optimización de sistemas de guía en tándem, métodos y composiciones para la manipulación de secuencias
US11306328B2 (en) 2013-07-26 2022-04-19 President And Fellows Of Harvard College Genome engineering
US9228207B2 (en) 2013-09-06 2016-01-05 President And Fellows Of Harvard College Switchable gRNAs comprising aptamers
ES2844174T3 (es) 2013-09-18 2021-07-21 Kymab Ltd Métodos, células y organismos
LT3066201T (lt) 2013-11-07 2018-08-10 Editas Medicine, Inc. Su crispr susiję būdai ir kompozicijos su valdančiomis grnr
EP3071695A2 (en) 2013-11-18 2016-09-28 Crispr Therapeutics AG Crispr-cas system materials and methods
WO2015089354A1 (en) 2013-12-12 2015-06-18 The Broad Institute Inc. Compositions and methods of use of crispr-cas systems in nucleotide repeat disorders
WO2015089486A2 (en) 2013-12-12 2015-06-18 The Broad Institute Inc. Systems, methods and compositions for sequence manipulation with optimized functional crispr-cas systems
MX2016007327A (es) * 2013-12-12 2017-03-06 Broad Inst Inc Suministro, uso y aplicaciones terapeuticas de sistemas y composiciones crispr-cas para dirigirlos a trastornos y enfermedades usando componentes para suministro de particulas.
WO2015089473A1 (en) 2013-12-12 2015-06-18 The Broad Institute Inc. Engineering of systems, methods and optimized guide compositions with new architectures for sequence manipulation
WO2015089364A1 (en) 2013-12-12 2015-06-18 The Broad Institute Inc. Crystal structure of a crispr-cas system, and uses thereof
WO2015086795A1 (en) 2013-12-13 2015-06-18 Cellectis Cas9 nuclease platform for microalgae genome engineering
WO2015109752A1 (en) 2014-01-21 2015-07-30 The Institute Of Genetics And Developmental Biology Chinese Academy Of Sciences Modified plants
JP5937635B2 (ja) 2014-03-28 2016-06-22 ファナック株式会社 電磁接触器の溶着検出機能を有するモータ駆動装置
CN104017821B (zh) * 2014-05-16 2016-07-06 安徽省农业科学院水稻研究所 定向编辑颖壳颜色决定基因OsCHI创制褐壳水稻材料的方法
WO2016049258A2 (en) 2014-09-25 2016-03-31 The Broad Institute Inc. Functional screening with optimized functional crispr-cas systems
WO2016094872A1 (en) 2014-12-12 2016-06-16 The Broad Institute Inc. Dead guides for crispr transcription factors
GB201506509D0 (en) 2015-04-16 2015-06-03 Univ Wageningen Nuclease-mediated genome editing
US20160362667A1 (en) 2015-06-10 2016-12-15 Caribou Biosciences, Inc. CRISPR-Cas Compositions and Methods
US9790490B2 (en) 2015-06-18 2017-10-17 The Broad Institute Inc. CRISPR enzymes and systems
CA3012631A1 (en) * 2015-06-18 2016-12-22 The Broad Institute Inc. Novel crispr enzymes and systems
WO2016205749A1 (en) * 2015-06-18 2016-12-22 The Broad Institute Inc. Novel crispr enzymes and systems
CA2998287A1 (en) 2015-09-24 2017-04-20 Crispr Therapeutics Ag Novel family of rna-programmable endonucleases and their uses in genome editing and other applications
US20190233814A1 (en) 2015-12-18 2019-08-01 The Broad Institute, Inc. Novel crispr enzymes and systems
CN105602993A (zh) * 2016-01-19 2016-05-25 上海赛墨生物技术有限公司 线粒体靶向的基因编辑系统及方法
AU2017257274B2 (en) 2016-04-19 2023-07-13 Massachusetts Institute Of Technology Novel CRISPR enzymes and systems
WO2021133977A1 (en) 2019-12-23 2021-07-01 The Broad Institute, Inc. Programmable dna nuclease-associated ligase and methods of use thereof

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