MX2018014993A - Uso de endonucleasa cpf1 para modificaciones de genoma de planta. - Google Patents
Uso de endonucleasa cpf1 para modificaciones de genoma de planta.Info
- Publication number
- MX2018014993A MX2018014993A MX2018014993A MX2018014993A MX2018014993A MX 2018014993 A MX2018014993 A MX 2018014993A MX 2018014993 A MX2018014993 A MX 2018014993A MX 2018014993 A MX2018014993 A MX 2018014993A MX 2018014993 A MX2018014993 A MX 2018014993A
- Authority
- MX
- Mexico
- Prior art keywords
- plant
- genome
- cpf1
- cpf1 endonuclease
- plant genome
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
Se proporcionan composiciones y métodos para la modificación gentotica de una secuencia diana en el genoma de una planta o célula vegetal, para la edición genonica de una secuencia de nucleótldos en el gen", de una planta o célula vegetal, y/o para Insertar o delecionar un polinucleótido de interés en o procedente del gano= da una planta. Loa métodos y composiciones emplean un sistema de polinucleótido de gulatandonuclease Cpfl para proporcionar un sistema eficaz para modificar o alterar sitios diana dentro del genes de una planta, célula vegetal o semilla. Además se proporcionan complejos de polinucleótidos de quia/endonucleasas Cpfl, polinucleotidos de quia, secuencias de endonucleaaa Cpfl optimizadas para plantas, construcciones de ADN reconbinante quo comprenden genes da endonuclossaa Cpfl optimizados para plantas, y combinaciones de los mismos.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201662349826P | 2016-06-14 | 2016-06-14 | |
US201762468013P | 2017-03-07 | 2017-03-07 | |
PCT/US2017/035175 WO2017218185A1 (en) | 2016-06-14 | 2017-05-31 | Use of cpf1 endonuclease for plant genome modifications |
Publications (1)
Publication Number | Publication Date |
---|---|
MX2018014993A true MX2018014993A (es) | 2019-09-06 |
Family
ID=59067902
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2018014993A MX2018014993A (es) | 2016-06-14 | 2017-05-31 | Uso de endonucleasa cpf1 para modificaciones de genoma de planta. |
Country Status (11)
Country | Link |
---|---|
US (1) | US20200332305A1 (es) |
EP (1) | EP3469077A1 (es) |
KR (1) | KR20190016970A (es) |
CN (1) | CN109312317A (es) |
AU (1) | AU2017286122A1 (es) |
BR (1) | BR112018076027A2 (es) |
CA (1) | CA3022854A1 (es) |
IL (1) | IL262795A (es) |
MX (1) | MX2018014993A (es) |
WO (1) | WO2017218185A1 (es) |
ZA (1) | ZA201807366B (es) |
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US9228207B2 (en) | 2013-09-06 | 2016-01-05 | President And Fellows Of Harvard College | Switchable gRNAs comprising aptamers |
US9526784B2 (en) | 2013-09-06 | 2016-12-27 | President And Fellows Of Harvard College | Delivery system for functional nucleases |
US9388430B2 (en) | 2013-09-06 | 2016-07-12 | President And Fellows Of Harvard College | Cas9-recombinase fusion proteins and uses thereof |
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CA2956224A1 (en) | 2014-07-30 | 2016-02-11 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
EP3553178A1 (en) | 2015-03-27 | 2019-10-16 | E. I. du Pont de Nemours and Company | Soybean u6 small nuclear rna gene promoters and their use in constitutive expression of small rna genes in plants |
US20190225955A1 (en) | 2015-10-23 | 2019-07-25 | President And Fellows Of Harvard College | Evolved cas9 proteins for gene editing |
WO2018027078A1 (en) | 2016-08-03 | 2018-02-08 | President And Fellows Of Harard College | Adenosine nucleobase editors and uses thereof |
AU2017308889B2 (en) | 2016-08-09 | 2023-11-09 | President And Fellows Of Harvard College | Programmable Cas9-recombinase fusion proteins and uses thereof |
US11542509B2 (en) | 2016-08-24 | 2023-01-03 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
KR20240007715A (ko) | 2016-10-14 | 2024-01-16 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 핵염기 에디터의 aav 전달 |
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CN110283840B (zh) * | 2019-04-11 | 2021-04-13 | 华中农业大学 | 陆地棉基因组的精确高效编辑方法 |
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2017
- 2017-05-31 CN CN201780037422.8A patent/CN109312317A/zh active Pending
- 2017-05-31 WO PCT/US2017/035175 patent/WO2017218185A1/en unknown
- 2017-05-31 US US16/305,260 patent/US20200332305A1/en not_active Abandoned
- 2017-05-31 KR KR1020187036654A patent/KR20190016970A/ko not_active Application Discontinuation
- 2017-05-31 CA CA3022854A patent/CA3022854A1/en not_active Abandoned
- 2017-05-31 MX MX2018014993A patent/MX2018014993A/es unknown
- 2017-05-31 EP EP17730618.0A patent/EP3469077A1/en active Pending
- 2017-05-31 BR BR112018076027-7A patent/BR112018076027A2/pt not_active Application Discontinuation
- 2017-05-31 AU AU2017286122A patent/AU2017286122A1/en not_active Abandoned
-
2018
- 2018-11-02 ZA ZA2018/07366A patent/ZA201807366B/en unknown
- 2018-11-05 IL IL262795A patent/IL262795A/en unknown
Also Published As
Publication number | Publication date |
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CA3022854A1 (en) | 2017-12-21 |
CN109312317A (zh) | 2019-02-05 |
ZA201807366B (en) | 2019-06-26 |
EP3469077A1 (en) | 2019-04-17 |
WO2017218185A1 (en) | 2017-12-21 |
US20200332305A1 (en) | 2020-10-22 |
KR20190016970A (ko) | 2019-02-19 |
BR112018076027A2 (pt) | 2019-03-26 |
IL262795A (en) | 2018-12-31 |
AU2017286122A1 (en) | 2018-11-22 |
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