BR112017000621A2 - método para melhorar um traço agronômico de uma planta, método para melhorar a produtividade de grãos ou a eficiência de uso de nitrogênio de uma planta de milho ou de cultura, método para editar, substituir ou eliminar uma sequência reguladora, método para inserir um promotor ou elemento do promotor no genoma de uma célula vegetal e método para fornecer um perfil de expressão adicional para um polinucleotídeo endógeno de uma célula vegetal - Google Patents
método para melhorar um traço agronômico de uma planta, método para melhorar a produtividade de grãos ou a eficiência de uso de nitrogênio de uma planta de milho ou de cultura, método para editar, substituir ou eliminar uma sequência reguladora, método para inserir um promotor ou elemento do promotor no genoma de uma célula vegetal e método para fornecer um perfil de expressão adicional para um polinucleotídeo endógeno de uma célula vegetalInfo
- Publication number
- BR112017000621A2 BR112017000621A2 BR112017000621A BR112017000621A BR112017000621A2 BR 112017000621 A2 BR112017000621 A2 BR 112017000621A2 BR 112017000621 A BR112017000621 A BR 112017000621A BR 112017000621 A BR112017000621 A BR 112017000621A BR 112017000621 A2 BR112017000621 A2 BR 112017000621A2
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- Prior art keywords
- plant
- plant cell
- improving
- promoter
- genome
- Prior art date
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- 238000000034 method Methods 0.000 title abstract 9
- 230000009418 agronomic effect Effects 0.000 title abstract 3
- 241000196324 Embryophyta Species 0.000 title 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 title 2
- 240000008042 Zea mays Species 0.000 title 1
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 title 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 title 1
- 235000013339 cereals Nutrition 0.000 title 1
- 244000038559 crop plants Species 0.000 title 1
- 235000009973 maize Nutrition 0.000 title 1
- 229910052757 nitrogen Inorganic materials 0.000 title 1
- 102000040430 polynucleotide Human genes 0.000 title 1
- 108091033319 polynucleotide Proteins 0.000 title 1
- 239000002157 polynucleotide Substances 0.000 title 1
- 230000001105 regulatory effect Effects 0.000 title 1
- 239000000203 mixture Substances 0.000 abstract 3
- 102000004533 Endonucleases Human genes 0.000 abstract 2
- 108010042407 Endonucleases Proteins 0.000 abstract 2
- 108020005004 Guide RNA Proteins 0.000 abstract 1
- 238000009402 cross-breeding Methods 0.000 abstract 1
- 238000012986 modification Methods 0.000 abstract 1
- 230000004048 modification Effects 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
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- C12N15/8249—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving ethylene biosynthesis, senescence or fruit development, e.g. modified tomato ripening, cut flower shelf-life
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- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
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- C12N15/09—Recombinant DNA-technology
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- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
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- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
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- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
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Abstract
composições e métodos são fornecidos para a modificação de traços agronômicos de uma sequência alvo no genoma de uma planta ou célula vegetal. os métodos e composições empregam um sistema de rna guia/endonuclease cas para fornecer um sistema eficaz para modificar ou alterar sítios alvo dentro de uma região genômica de uma planta, célula vegetal ou semente, para fornecer o aprimoramento em um traço agronômico desejado como seca, produtividade e tolerância ao estresse. métodos de cruzamento para selecionar plantas utilizando um sistema de dois componentes, de rna guia e endonuclease cas, também são revelados. composições e métodos são apresentados, também, para editar uma sequência de nucleotídeos no genoma de uma célula.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
BR122023024818-0A BR122023024818A2 (pt) | 2014-07-11 | 2015-07-13 | Rna guia, polinucleotídeo e complexo de ribonucleoproteínas |
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201462023239P | 2014-07-11 | 2014-07-11 | |
US62/023,239 | 2014-07-11 | ||
PCT/US2015/040143 WO2016007948A1 (en) | 2014-07-11 | 2015-07-13 | Agronomic trait modification using guide rna/cas endonuclease systems and methods of use |
Publications (2)
Publication Number | Publication Date |
---|---|
BR112017000621A2 true BR112017000621A2 (pt) | 2018-01-23 |
BR112017000621B1 BR112017000621B1 (pt) | 2024-03-12 |
Family
ID=55065013
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR112017000621-9A BR112017000621B1 (pt) | 2014-07-11 | 2015-07-13 | Método para melhorar um traço agronômico de uma planta de milho ou de soja |
BR122023024818-0A BR122023024818A2 (pt) | 2014-07-11 | 2015-07-13 | Rna guia, polinucleotídeo e complexo de ribonucleoproteínas |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
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BR122023024818-0A BR122023024818A2 (pt) | 2014-07-11 | 2015-07-13 | Rna guia, polinucleotídeo e complexo de ribonucleoproteínas |
Country Status (5)
Country | Link |
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US (2) | US20170183677A1 (pt) |
CN (1) | CN106795524A (pt) |
BR (2) | BR112017000621B1 (pt) |
CA (1) | CA2954686A1 (pt) |
WO (1) | WO2016007948A1 (pt) |
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UA119135C2 (uk) * | 2012-09-07 | 2019-05-10 | ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі | Спосіб отримання трансгенної рослини |
US20150291967A1 (en) * | 2012-10-31 | 2015-10-15 | Luc Mathis | Coupling herbicide resistance with targeted insertion of transgenes in plants |
EP3825401A1 (en) * | 2012-12-12 | 2021-05-26 | The Broad Institute, Inc. | Crispr-cas component systems, methods and compositions for sequence manipulation |
MX2015007550A (es) * | 2012-12-12 | 2017-02-02 | Broad Inst Inc | Suministro, modificación y optimización de sistemas, métodos y composiciones para la manipulación de secuencias y aplicaciones terapéuticas. |
CN103667338B (zh) * | 2013-11-28 | 2016-01-27 | 中国科学院遗传与发育生物学研究所 | 一种玉米基因组定点改造方法 |
CA2937429A1 (en) * | 2014-01-21 | 2015-07-30 | Caixia Gao | Modified plants |
-
2015
- 2015-07-13 CN CN201580049070.9A patent/CN106795524A/zh active Pending
- 2015-07-13 WO PCT/US2015/040143 patent/WO2016007948A1/en active Application Filing
- 2015-07-13 CA CA2954686A patent/CA2954686A1/en active Pending
- 2015-07-13 US US15/323,772 patent/US20170183677A1/en not_active Abandoned
- 2015-07-13 BR BR112017000621-9A patent/BR112017000621B1/pt active IP Right Grant
- 2015-07-13 BR BR122023024818-0A patent/BR122023024818A2/pt not_active Application Discontinuation
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2022
- 2022-03-25 US US17/656,594 patent/US20220364107A1/en active Pending
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BR112017000621B1 (pt) | 2024-03-12 |
US20170183677A1 (en) | 2017-06-29 |
CA2954686A1 (en) | 2016-01-14 |
WO2016007948A1 (en) | 2016-01-14 |
US20220364107A1 (en) | 2022-11-17 |
CN106795524A (zh) | 2017-05-31 |
BR122023024818A2 (pt) | 2023-12-26 |
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