BR112018007351A2 - métodos de seleção de células e levedura - Google Patents

métodos de seleção de células e levedura

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Publication number
BR112018007351A2
BR112018007351A2 BR112018007351A BR112018007351A BR112018007351A2 BR 112018007351 A2 BR112018007351 A2 BR 112018007351A2 BR 112018007351 A BR112018007351 A BR 112018007351A BR 112018007351 A BR112018007351 A BR 112018007351A BR 112018007351 A2 BR112018007351 A2 BR 112018007351A2
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Brazil
Prior art keywords
methods
cell
genome
selection methods
polynucleotide
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BR112018007351A
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English (en)
Inventor
L Frisch Ryan
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Du Pont
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Application filed by Du Pont filed Critical Du Pont
Publication of BR112018007351A2 publication Critical patent/BR112018007351A2/pt

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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/905—Stable introduction of foreign DNA into chromosome using homologous recombination in yeast
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    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
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    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites

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Abstract

são fornecidas composições e métodos de modificação de sequências de nucleotídeos no genoma de células. os métodos e composições empregam um polinucleotídeo guia, um modelo de modificação de polinucleotídeos protegido e cas endonuclease para modificar uma sequência de nucleotídeos e/ou aumentar a frequência de reparo dirigido homólogo. os métodos podem ser adicionalmente utilizados para reduzir a frequência de integração fora do local de qualquer modelo de modificação. a presente invenção também descreve métodos de seleção de células que compreendem um local alvo modificado no seu genoma e métodos de seleção de células que compreendem um polinucleotídeo de interesse inserido em um local alvo no seu genoma.
BR112018007351A 2015-10-12 2016-10-11 métodos de seleção de células e levedura BR112018007351A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562240140P 2015-10-12 2015-10-12
PCT/US2016/056404 WO2017066175A1 (en) 2015-10-12 2016-10-11 Protected dna templates for gene modification and increased homologous recombination in cells and methods of use

Publications (1)

Publication Number Publication Date
BR112018007351A2 true BR112018007351A2 (pt) 2018-10-23

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Application Number Title Priority Date Filing Date
BR112018007351A BR112018007351A2 (pt) 2015-10-12 2016-10-11 métodos de seleção de células e levedura

Country Status (9)

Country Link
US (1) US20180273979A1 (pt)
EP (2) EP4144844A1 (pt)
JP (1) JP7011590B2 (pt)
KR (1) KR102628801B1 (pt)
AU (1) AU2016338785B2 (pt)
BR (1) BR112018007351A2 (pt)
CA (1) CA2999050A1 (pt)
DK (1) DK3362560T3 (pt)
WO (1) WO2017066175A1 (pt)

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