SG11201900907YA - Adenosine nucleobase editors and uses thereof - Google Patents

Adenosine nucleobase editors and uses thereof

Info

Publication number
SG11201900907YA
SG11201900907YA SG11201900907YA SG11201900907YA SG11201900907YA SG 11201900907Y A SG11201900907Y A SG 11201900907YA SG 11201900907Y A SG11201900907Y A SG 11201900907YA SG 11201900907Y A SG11201900907Y A SG 11201900907YA SG 11201900907Y A SG11201900907Y A SG 11201900907YA
Authority
SG
Singapore
Prior art keywords
international
adenosine
ma
pct
publication
Prior art date
Application number
SG11201900907YA
Inventor
David Liu
Nicole Gaudelli
Original Assignee
Harvard College
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to US201662370684P priority Critical
Priority to US201762454035P priority
Priority to US201762473714P priority
Application filed by Harvard College filed Critical Harvard College
Priority to PCT/US2017/045381 priority patent/WO2018027078A1/en
Publication of SG11201900907YA publication Critical patent/SG11201900907YA/en

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102Mutagenizing nucleic acids
    • C12N15/1024In vivo mutagenesis using high mutation rate "mutator" host strains by inserting genetic material, e.g. encoding an error prone polymerase, disrupting a gene for mismatch repair
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22Ribonucleases RNAses, DNAses
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/78Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y305/00Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/04Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in cyclic amidines (3.5.4)
    • C12Y305/04004Adenosine deaminase (3.5.4.4)

Abstract

International Publication Date (19) World Intellectual Property Organization International Bureau 1111111111110110101011111011010111101 M HO I HIMEMOU (10) International Publication Number WO 2018/027078 A8 (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) CORRECTED VERSION 08 February 2018 (08.02.2018) WI PO I PCT co co N O N O co O 1-1 C (51) International Patent Classification: C12N 9/78 (2006.01) (21) International Application Number: PCT/US2017/045381 (22) International Filing Date: 03 August 2017 (03.08.2017) (25) Filing Language: English (26) Publication Language: English (30) Priority Data: 62/370,684 03 August 2016 (03.08.2016) US 62/454,035 02 February 2017 (02.02.2017) US 62/473,714 20 March 2017 (20.03.2017) US (71) Applicant: PRESIDENT AND FELLOWS OF HAR- VARD COLLEGE [US/US]; 17 Quincy Street, Cam- bridge, MD 02138 (US). (72) Inventors: LIU, David, R.; 3 Whitman Circle, Lexington, MA 02420 (US). GAUDELLI, Nicole; 44 Bartlett Avenue, Belmont, MA 02478 (US). (74) Agent: HEBERT, Alan, M.; Wold, Greenfield & Sacks, P.C., 600 Atlantic Avenue, Boston, MA 02210-22006 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JO, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: with international search report (Art. 21(3)) with sequence listing part of description (Rule 5.2(a)) (48) Date of publication of this corrected version: 06 December 2018 (06.12.2018) (15) Information about Correction: see Notice of 06 December 2018 (06.12.2018) (54) Title: ADENOSINE NUCLEOBASE EDITORS AND USES THEREOF (57) : The disclosure provides adenosine deaminases that are capable of deaminating adenosine in DNA. The disclosure also provides fusion proteins comprising a Cas9 (e.g., a Cas9 nickase) domain and adenosine deaminases that deaminate adenosine in DNA. In some embodiments, the fusion proteins further comprise a nuclear localization sequence (NLS), and/or an inhibitor of base repair, such as, a nuclease dead inosine specific nuclease (dISN).
SG11201900907YA 2016-08-03 2017-08-03 Adenosine nucleobase editors and uses thereof SG11201900907YA (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
US201662370684P true 2016-08-03 2016-08-03
US201762454035P true 2017-02-02 2017-02-02
US201762473714P true 2017-03-20 2017-03-20
PCT/US2017/045381 WO2018027078A1 (en) 2016-08-03 2017-08-03 Adenosine nucleobase editors and uses thereof

Publications (1)

Publication Number Publication Date
SG11201900907YA true SG11201900907YA (en) 2019-02-27

Family

ID=59714096

Family Applications (1)

Application Number Title Priority Date Filing Date
SG11201900907YA SG11201900907YA (en) 2016-08-03 2017-08-03 Adenosine nucleobase editors and uses thereof

Country Status (9)

Country Link
US (2) US10113163B2 (en)
EP (1) EP3494215A1 (en)
KR (1) KR20190034628A (en)
AU (1) AU2017306676A1 (en)
CA (1) CA3032699A1 (en)
GB (1) GB2568182A (en)
IL (1) IL264565D0 (en)
SG (1) SG11201900907YA (en)
WO (1) WO2018027078A1 (en)

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CA2853829A1 (en) 2011-07-22 2013-05-10 President And Fellows Of Harvard College Evaluation and improvement of nuclease cleavage specificity
SG11201900907YA (en) 2016-08-03 2019-02-27 Harvard College Adenosine nucleobase editors and uses thereof
WO2018208998A1 (en) * 2017-05-10 2018-11-15 The Regents Of The University Of California Directed editing of cellular rna via nuclear delivery of crispr/cas9
WO2018209320A1 (en) 2017-05-12 2018-11-15 President And Fellows Of Harvard College Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation
WO2019023680A1 (en) 2017-07-28 2019-01-31 President And Fellows Of Harvard College Methods and compositions for evolving base editors using phage-assisted continuous evolution (pace)
WO2019079347A1 (en) * 2017-10-16 2019-04-25 The Broad Institute, Inc. Uses of adenosine base editors

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