CN206970581U - A kind of kit for being used to aid in CRISPR/cas9 gene knockouts - Google Patents
A kind of kit for being used to aid in CRISPR/cas9 gene knockouts Download PDFInfo
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- CN206970581U CN206970581U CN201720445661.4U CN201720445661U CN206970581U CN 206970581 U CN206970581 U CN 206970581U CN 201720445661 U CN201720445661 U CN 201720445661U CN 206970581 U CN206970581 U CN 206970581U
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- kit
- crispr
- aid
- gene knockouts
- cas9 gene
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Abstract
A kind of container is the utility model is related to, specifically a kind of kit for being used to aid in CRISPR/cas9 gene knockouts.The kit includes lid, box body, fixed seat, reagent bottle and pipette tip box.The fixed seat is fixed on kit bottom part down, its upper surface hole, is easy to fix container, and the pore quantity of fixed seat can be adjusted according to the number of Kit Contents.There is double-stranded RNA in the reagent bottle, during using CRISPR/cas9 system targeting knock out genes, the cotransfection double-stranded RNA, can effectively improve the non-homogeneous restructuring of target gene（NHEJ）Efficiency, pipette tip box have liquid transfer gun head, wherein containing without RNase liquid transfer gun head, 200 μ L-types number and 10 μ L-types number are respectively 10.
Description
Technical field
A kind of container is the utility model is related to, specifically a kind of kit technology of CRISPR/cas9 gene knockouts auxiliary
Field.
Background technology
It the utility model is related to a kind of kit for being used to aid in CRISPR/cas9 gene knockouts.Specifically, this practicality
Novel agent box, equipped with the double-stranded RNA for filtering out efficiently interference pten gene expressions.Struck using the targeting of CRISPR/cas9 systems
During except gene, the cotransfection double-stranded RNA, the non-homogeneous restructuring of target gene can be effectively improved（NHEJ）Efficiency.In multiple targets
Point checking, is significantly improved, this method cost is low, simple to operate, efficiency high.For CRISPR/cas9 technologies efficiency and
Using with good facilitation.
The content of the invention
The purpose of this utility model is to provide a kind of kit for being used to aid in CRISPR/cas9 gene knockouts.
The utility model is achieved in the following ways：
A kind of container is the utility model is related to, specifically a kind of kit for being used to aid in CRISPR/cas9 gene knockouts.
The kit includes lid, box body, fixed seat, reagent bottle and pipette tip box.The fixed seat is fixed under kit bottom
Side, its upper surface hole, is easy to fix container, according to Kit Contents number can adjust the hole number of fixed seat
Amount.The pipette tip box is fixed on box body fixed seat at bottom, equipped with liquid transfer gun head, wherein containing without RNase liquid transfer gun head, 200
μ L and each 10 of 10 μ L-type liquid transfer gun heads.The shape of above-mentioned box body is not particularly limited, and can be cuboid, square and circle
Cylinder etc., preferably cuboid.
Above-mentioned lid can be connected with box body and also be not connected to, and according to the characteristics of content and can be easy to the requirement picked and placeed
To design, the side connection of preferably described lid and box body, both it is easy to pick and place article, box will not be caused in transportation again
Lid comes off.
The size of the box body is not particularly limited with height, can be preferably according to how much adjustment of actual content
Long 12-18cm, wide 8-14cm, high 10-16cm.
Above-mentioned fixed seat is placed on the bottom of box body, and shape size matches with box body bottom.
The fixed seat is highly less than box body height, it is preferable that the height of fixed seat is no more than a quarter of box body.
Above-mentioned fixed seat upper surface is provided with the hole being coincide with the container bottom size and shape of preloaded, is held with facilitating
Device insertion is fixed.It can be arranged, be evenly distributed in fixed seat with identical spacing between hole, can be according to different size, height
Degree or the container of specification set the depth of hole, and the preferably depth of hole is no more than the half of liquid container height.
Above-mentioned pipette tip box, its size with height be not particularly limited, can according to how much adjustment of actual content,
Preferably long 8-10cm, wide 5-8cm, high 5-8cm
Above-mentioned pipette tip box, built with without RNase liquid transfer gun head, 200 μ L-types number are 10,10 μ L-types number are 10,
Clean level is that the detailed design of pipette tips box is with reference to patent 201720274182.0 without RNase rank.
Brief description of the drawings
Fig. 1 is the reagent cartridge configuration schematic diagram of one embodiment of utility model
Main Reference label declaration（1st, lid 2, box body 3, fixed seat 4, hole 5, reagent bottle 6, pipette tip box
7th, operational manual）.
Embodiment
Specific embodiment of the present utility model is described in detail below in conjunction with accompanying drawing.Those skilled in the art will
Understand, the following examples are merely to illustrate the utility model, and should not be regarded as limiting the scope of the utility model.
Embodiment one
There is provided it is a kind of can be used for auxiliary CRISPR/cas9 gene knockouts kit, the kit specifically include lid 1,
Box body 2, fixed seat 3, hole 4, reagent bottle 5, pipette tips box 6, operational manual 7, its embodiment is with reference to patent
201710155251.0。
Kit is cuboid in the present embodiment, long 18cm, wide 12cm, high 15cm.
Lid 1 is connected with box body 2 in a fixed manner.
Fixed seat 3 is positioned over the bottom of box body 2, and size shape matches with the bottom of box body 2, highly four for box body 2
/ mono-.Hole 4 is uniformly distributed in fixed seat 3, and depth is the half of the height of reagent bottle 5, shape and reagent bottle 5
Match, facilitate the insertion of reagent bottle 5 to fix.
There is a long 12cm upper surface of fixed seat 3, wide 5cm, high 10cm pipette tips box 6, with fixed seat 3 removably
Connection.
Reagent bottle 4, this kit are equipped with 1uM double-stranded RNAs built with two reagent bottles, reagent bottle one, are 3 double-strands
The 1 of RNA：1：1 mixing（With reference to patent 201710155251.0）, another reagent bottle be equipped with without RNase water 10ml, be used for
Dilute double-stranded RNA.
Claims (8)
- A kind of 1. kit for being used to aid in CRISPR/cas9 gene knockouts, it is characterised in that：Including lid, box body, fixation Seat, reagent bottle and pipette tip box, the fixed seat are fixed on kit bottom part down, its upper surface hole, are easy to fix Container, the pore quantity of fixed seat can be adjusted according to the number of Kit Contents.
- 2. a kind of kit for being used to aid in CRISPR/cas9 gene knockouts according to claim 1, its feature exist In：The box body is cuboid, square or cylinder.
- 3. a kind of kit for being used to aid in CRISPR/cas9 gene knockouts according to claim 1, its feature exist In：The lid can be connected with box body and also be not connected to.
- 4. a kind of kit for being used to aid in CRISPR/cas9 gene knockouts according to claim 1, its feature exist In：The side connection of the lid and box body.
- 5. a kind of kit for being used to aid in CRISPR/cas9 gene knockouts according to claim 1, its feature exist In：The reagent bottle is fixed in base hole, and its quantity is 2-10.
- 6. a kind of kit for being used to aid in CRISPR/cas9 gene knockouts according to claim 1, its feature exist In：The pipette tip box is equipped with liquid transfer gun head 20,200 μ L and each 10 of 10 μ L-type liquid transfer gun heads.
- 7. kit according to claim 6, clean level is without RNase rank.
- 8. kit according to claim 1, there is two reagent bottles in this kit, a reagent bottle equipped with 10ml without RNase water, another reagent bottle are equipped with 1uM siRNAs.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720445661.4U CN206970581U (en) | 2017-04-26 | 2017-04-26 | A kind of kit for being used to aid in CRISPR/cas9 gene knockouts |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720445661.4U CN206970581U (en) | 2017-04-26 | 2017-04-26 | A kind of kit for being used to aid in CRISPR/cas9 gene knockouts |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206970581U true CN206970581U (en) | 2018-02-06 |
Family
ID=61411485
Family Applications (1)
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| CN201720445661.4U Active CN206970581U (en) | 2017-04-26 | 2017-04-26 | A kind of kit for being used to aid in CRISPR/cas9 gene knockouts |
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Cited By (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10323236B2 (en) | 2011-07-22 | 2019-06-18 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
| US10465176B2 (en) | 2013-12-12 | 2019-11-05 | President And Fellows Of Harvard College | Cas variants for gene editing |
| US10508298B2 (en) | 2013-08-09 | 2019-12-17 | President And Fellows Of Harvard College | Methods for identifying a target site of a CAS9 nuclease |
| US10597679B2 (en) | 2013-09-06 | 2020-03-24 | President And Fellows Of Harvard College | Switchable Cas9 nucleases and uses thereof |
| US10682410B2 (en) | 2013-09-06 | 2020-06-16 | President And Fellows Of Harvard College | Delivery system for functional nucleases |
| US10704062B2 (en) | 2014-07-30 | 2020-07-07 | President And Fellows Of Harvard College | CAS9 proteins including ligand-dependent inteins |
| US10745677B2 (en) | 2016-12-23 | 2020-08-18 | President And Fellows Of Harvard College | Editing of CCR5 receptor gene to protect against HIV infection |
| US10858639B2 (en) | 2013-09-06 | 2020-12-08 | President And Fellows Of Harvard College | CAS9 variants and uses thereof |
| US10947530B2 (en) | 2016-08-03 | 2021-03-16 | President And Fellows Of Harvard College | Adenosine nucleobase editors and uses thereof |
| US11046948B2 (en) | 2013-08-22 | 2021-06-29 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
| US11214780B2 (en) | 2015-10-23 | 2022-01-04 | President And Fellows Of Harvard College | Nucleobase editors and uses thereof |
| US11268082B2 (en) | 2017-03-23 | 2022-03-08 | President And Fellows Of Harvard College | Nucleobase editors comprising nucleic acid programmable DNA binding proteins |
| US11306324B2 (en) | 2016-10-14 | 2022-04-19 | President And Fellows Of Harvard College | AAV delivery of nucleobase editors |
| US11319532B2 (en) | 2017-08-30 | 2022-05-03 | President And Fellows Of Harvard College | High efficiency base editors comprising Gam |
| US11447770B1 (en) | 2019-03-19 | 2022-09-20 | The Broad Institute, Inc. | Methods and compositions for prime editing nucleotide sequences |
| US11542496B2 (en) | 2017-03-10 | 2023-01-03 | President And Fellows Of Harvard College | Cytosine to guanine base editor |
| US11542509B2 (en) | 2016-08-24 | 2023-01-03 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| US11560566B2 (en) | 2017-05-12 | 2023-01-24 | President And Fellows Of Harvard College | Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation |
| US11661590B2 (en) | 2016-08-09 | 2023-05-30 | President And Fellows Of Harvard College | Programmable CAS9-recombinase fusion proteins and uses thereof |
| US11732274B2 (en) | 2017-07-28 | 2023-08-22 | President And Fellows Of Harvard College | Methods and compositions for evolving base editors using phage-assisted continuous evolution (PACE) |
| US11795443B2 (en) | 2017-10-16 | 2023-10-24 | The Broad Institute, Inc. | Uses of adenosine base editors |
| US11898179B2 (en) | 2017-03-09 | 2024-02-13 | President And Fellows Of Harvard College | Suppression of pain by gene editing |
| US11912985B2 (en) | 2020-05-08 | 2024-02-27 | The Broad Institute, Inc. | Methods and compositions for simultaneous editing of both strands of a target double-stranded nucleotide sequence |
-
2017
- 2017-04-26 CN CN201720445661.4U patent/CN206970581U/en active Active
Cited By (35)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10323236B2 (en) | 2011-07-22 | 2019-06-18 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
| US10954548B2 (en) | 2013-08-09 | 2021-03-23 | President And Fellows Of Harvard College | Nuclease profiling system |
| US11920181B2 (en) | 2013-08-09 | 2024-03-05 | President And Fellows Of Harvard College | Nuclease profiling system |
| US10508298B2 (en) | 2013-08-09 | 2019-12-17 | President And Fellows Of Harvard College | Methods for identifying a target site of a CAS9 nuclease |
| US11046948B2 (en) | 2013-08-22 | 2021-06-29 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
| US11299755B2 (en) | 2013-09-06 | 2022-04-12 | President And Fellows Of Harvard College | Switchable CAS9 nucleases and uses thereof |
| US10858639B2 (en) | 2013-09-06 | 2020-12-08 | President And Fellows Of Harvard College | CAS9 variants and uses thereof |
| US10912833B2 (en) | 2013-09-06 | 2021-02-09 | President And Fellows Of Harvard College | Delivery of negatively charged proteins using cationic lipids |
| US10682410B2 (en) | 2013-09-06 | 2020-06-16 | President And Fellows Of Harvard College | Delivery system for functional nucleases |
| US10597679B2 (en) | 2013-09-06 | 2020-03-24 | President And Fellows Of Harvard College | Switchable Cas9 nucleases and uses thereof |
| US10465176B2 (en) | 2013-12-12 | 2019-11-05 | President And Fellows Of Harvard College | Cas variants for gene editing |
| US11053481B2 (en) | 2013-12-12 | 2021-07-06 | President And Fellows Of Harvard College | Fusions of Cas9 domains and nucleic acid-editing domains |
| US11124782B2 (en) | 2013-12-12 | 2021-09-21 | President And Fellows Of Harvard College | Cas variants for gene editing |
| US11578343B2 (en) | 2014-07-30 | 2023-02-14 | President And Fellows Of Harvard College | CAS9 proteins including ligand-dependent inteins |
| US10704062B2 (en) | 2014-07-30 | 2020-07-07 | President And Fellows Of Harvard College | CAS9 proteins including ligand-dependent inteins |
| US11214780B2 (en) | 2015-10-23 | 2022-01-04 | President And Fellows Of Harvard College | Nucleobase editors and uses thereof |
| US11702651B2 (en) | 2016-08-03 | 2023-07-18 | President And Fellows Of Harvard College | Adenosine nucleobase editors and uses thereof |
| US10947530B2 (en) | 2016-08-03 | 2021-03-16 | President And Fellows Of Harvard College | Adenosine nucleobase editors and uses thereof |
| US11661590B2 (en) | 2016-08-09 | 2023-05-30 | President And Fellows Of Harvard College | Programmable CAS9-recombinase fusion proteins and uses thereof |
| US11542509B2 (en) | 2016-08-24 | 2023-01-03 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| US11306324B2 (en) | 2016-10-14 | 2022-04-19 | President And Fellows Of Harvard College | AAV delivery of nucleobase editors |
| US11820969B2 (en) | 2016-12-23 | 2023-11-21 | President And Fellows Of Harvard College | Editing of CCR2 receptor gene to protect against HIV infection |
| US10745677B2 (en) | 2016-12-23 | 2020-08-18 | President And Fellows Of Harvard College | Editing of CCR5 receptor gene to protect against HIV infection |
| US11898179B2 (en) | 2017-03-09 | 2024-02-13 | President And Fellows Of Harvard College | Suppression of pain by gene editing |
| US11542496B2 (en) | 2017-03-10 | 2023-01-03 | President And Fellows Of Harvard College | Cytosine to guanine base editor |
| US11268082B2 (en) | 2017-03-23 | 2022-03-08 | President And Fellows Of Harvard College | Nucleobase editors comprising nucleic acid programmable DNA binding proteins |
| US11560566B2 (en) | 2017-05-12 | 2023-01-24 | President And Fellows Of Harvard College | Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation |
| US11732274B2 (en) | 2017-07-28 | 2023-08-22 | President And Fellows Of Harvard College | Methods and compositions for evolving base editors using phage-assisted continuous evolution (PACE) |
| US11319532B2 (en) | 2017-08-30 | 2022-05-03 | President And Fellows Of Harvard College | High efficiency base editors comprising Gam |
| US11932884B2 (en) | 2017-08-30 | 2024-03-19 | President And Fellows Of Harvard College | High efficiency base editors comprising Gam |
| US11795443B2 (en) | 2017-10-16 | 2023-10-24 | The Broad Institute, Inc. | Uses of adenosine base editors |
| US11643652B2 (en) | 2019-03-19 | 2023-05-09 | The Broad Institute, Inc. | Methods and compositions for prime editing nucleotide sequences |
| US11795452B2 (en) | 2019-03-19 | 2023-10-24 | The Broad Institute, Inc. | Methods and compositions for prime editing nucleotide sequences |
| US11447770B1 (en) | 2019-03-19 | 2022-09-20 | The Broad Institute, Inc. | Methods and compositions for prime editing nucleotide sequences |
| US11912985B2 (en) | 2020-05-08 | 2024-02-27 | The Broad Institute, Inc. | Methods and compositions for simultaneous editing of both strands of a target double-stranded nucleotide sequence |
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