CN109874674A - Partition type plant tissue culture bottle - Google Patents

Partition type plant tissue culture bottle Download PDF

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Publication number
CN109874674A
CN109874674A CN201910274514.9A CN201910274514A CN109874674A CN 109874674 A CN109874674 A CN 109874674A CN 201910274514 A CN201910274514 A CN 201910274514A CN 109874674 A CN109874674 A CN 109874674A
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CN
China
Prior art keywords
isolation board
plant tissue
board
installation axle
culture bottle
Prior art date
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Pending
Application number
CN201910274514.9A
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Chinese (zh)
Inventor
张志勇
陈莹
江雪波
祝漂
李玲
曾燕羚
唐天明
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Neijiang Normal University
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Neijiang Normal University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Neijiang Normal University filed Critical Neijiang Normal University
Priority to CN201910274514.9A priority Critical patent/CN109874674A/en
Publication of CN109874674A publication Critical patent/CN109874674A/en
Pending legal-status Critical Current

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Abstract

The invention discloses partition type plant tissue culture bottles, relate generally to culture bottle field.Including basic module and functional module;The basic module includes the body and bottle cap being used in combination, and the body is that section is circular straight cylinder;The functional module includes installation axle, isolation board, mirror board, the installation axle is detachably mounted to the center inside body, the width and body inside radius of the isolation board are adapted, the inside of the isolation board is removably rotatablely connected with installation axle, the outside of the isolation board is in contact with body inner wall, mirror board is removably installed, the surface of the mirror board is equipped with the mirror surface with amplification factor, and the amplification factor is 2~8 times on the two sides side of the isolation board.The beneficial effects of the present invention are: it can carry out isolation culture to multiple lesser plant tissues, greatly save the use of culture bottle, and the culture resource saved including equipment, space, energy consumption etc. at double.

Description

Partition type plant tissue culture bottle
Technical field
The present invention relates to culture bottle fields, specifically partition type plant tissue culture bottle.
Background technique
Culture bottle is often used when plant tissue is cultivated in laboratory.It is typically necessary and sterile training is carried out to plant It supports.And in order to be worth with statistics, the individual amount of culture is often very more, could have directive significance in data.
Due to very small after many Plant Tissue Breeding, and culture bottle is to consider general and be convenient to clean, specification It is generally more unified.Therefore when largely cultivating individual lesser plant tissue, the culture bottle of unified specification is still used, not only directly The a large amount of uses for causing culture bottle are connect, a large amount of occupancy of culture space can also consume culture resource excessively --- such as shaking table, perseverance Warm device, illumination apparatus etc..The treating capacity of these equipment is all related with placed volume, the occupancy of such mass propgation bottle, Excessive culture resource will necessarily be consumed, and since the culture bottle of single treatment is limited, same incubation is caused to need to be grouped It repeatedly carries out, the amount of labour is very big, and efficiency is very low.
Summary of the invention
The purpose of the present invention is to provide partition type plant tissue culture bottle, it can to multiple lesser plant tissues into Row isolation culture, greatlys save the use of culture bottle, and the culture money saved including equipment, space, energy consumption etc. at double Source.
The present invention to achieve the above object, is achieved through the following technical solutions:
Partition type plant tissue culture bottle, including basic module and functional module;
The basic module includes the body and bottle cap being used in combination, and the body is that section is circular straight cylinder;
The functional module includes installation axle, isolation board, mirror board, and the installation axle is detachably mounted to inside body Center, the width of the isolation board and body inside radius be adapted, and the inside of the isolation board removably turns with installation axle Dynamic connection, the outside of the isolation board is in contact with body inner wall, is removably equipped on the two sides side of the isolation board Mirror board, the surface of the mirror board are equipped with the mirror surface with amplification factor, and the amplification factor is 2~8 times.
The interior bottom surface center of the body is equipped with inserting column, and the bottom end of the inserting column and installation axle is threadedly engaged.
The bottom surface center of the body is equipped with inserting column, and the bottom surface center of the bottle cap is equipped with socket, the bottom of the installation axle End and inserting column activity grafting, the top of the installation axle and socket activity grafting.
The inboard of the isolation board is equipped with c-type axle sleeve, the c-type axle sleeve and installation axle transition fit.
The surface of the installation axle is axially arranged with the stupefied muscle extended vertically along it, and the inner peripheral surface of the c-type axle sleeve is equipped with The stupefied slot being adapted with stupefied muscle.
It is relatively isolated the difference of plate height according to the c-type axle sleeve, sets different model for isolation board.
The functional module further includes marking plate, and the side of the marking plate is equipped with the first slotting axis and second in parallel Axis is inserted, in the same plane, the second slotting wheelbase is vertical from the plane where marking plate for the first slotting axis and marking plate Distance is 0.5~1.5cm, and the top side of the isolation board is equipped with and can be inserted and rotated and be matched by the first slotting axis or the second slotting axis activity The annular element of conjunction.
The thickness of the mirror board top side is greater than the thickness of its bottom side, its mirror after being mounted on the mirror board on isolation board Face is inclined surface.
The prior art is compared, the beneficial effects of the present invention are:
1) specific aim of Plant Tissue Breeding is greatly enhanced, improves the observation in culture to plant tissue, improve experiment Quality and effect.
2) laboratory consumptive material and space resources are reduced.It can by the management to widgets and flexibly use using the present apparatus To realize the culture to different characteristics plant tissue.It, can at double by subregion when in face of the plant tissue compared with plantlet Culture bottle quantity is reduced, significantly saves occupied space resources, device resource, energy etc., it may be said that be greatly saved The consumptive material in laboratory, energy consumption, significantly reduce toxigenic capacity.
3) have versatility, be equipped with this culture bottle, just do not have to be equipped with other culture bottles, it can be in various situations Plant tissue it is general, not only be easy to use but also facilitate management.
Detailed description of the invention
Attached drawing 1 is that each component of the embodiment of the present invention 1 splits schematic diagram.
Attached drawing 2 is the isolation board schematic diagram of the embodiment of the present invention 1.
Attached drawing 3 is the use state diagram of the embodiment of the present invention 2.
Attached drawing 4 is the marking plate partial enlarged view (the first slotting axis insertion annular element) of the embodiment of the present invention 2.
Attached drawing 5 is the marking plate partial enlarged view (the second slotting axis insertion annular element) of the embodiment of the present invention 2.
Label shown in attached drawing:
1, body;2, bottle cap;3, inserting column;4, socket;5, installation axle;6, isolation board;7, mirror board;8, fastener;9, screw thread Slot;10, stupefied muscle;11, c-type axle sleeve;12, stupefied slot;13, tooth socket;14, arc-like sheet;15, marking plate;16, the first slotting axis;17, second Insert axis;18, annular element;19, baffle;20, low water.
Specific embodiment
Present invention will be further explained below with reference to specific examples.It should be understood that these embodiments are merely to illustrate the present invention Rather than it limits the scope of the invention.In addition, it should also be understood that, after reading the content taught by the present invention, those skilled in the art Member can make various changes or modifications the present invention, and such equivalent forms equally fall within range defined herein.
Embodiment 1: partition type plant tissue culture bottle.Partition subregion training is carried out mainly for the lesser plant tissue of plant It supports, and reinforces observing effect, after investment is widely applied, the indoor space utilization of experiment, planning management acquisition will significantly be changed Into, and greatly save the consumption to material.There is promotional value very much
In this example, under the original intention based on subregion, the popularity of this culture bottle application, avoids reality in order to better improve If testing room is being equipped with this culture bottle, must individually prepare routine culture product for big strain plant, thus this example sufficiently draw close it is logical With property, optional function is set by sectoring function, whole design realizes different functions by the mutual cooperation of component, corresponding Different culture demands.Specific structure is as follows:
It mainly include two modules --- basic module and functional module.
As its name suggests, basic module is for realizing culture bottle basic function --- the module of culture plant tissue function, The mainly specific structure of bottle body.In order to cooperate the mentality of designing of fanned partition, the culture bottle structure of circular body 1 is selected. And functional module is can to cooperate culture demand, alternative is superimposed upon the other assemblies of the enterprising enforcement of basic module.At this It is as follows to the concrete structure design of above-mentioned two module in example:
1) basic module
Including body 1 and bottle cap 2.The body 1 and bottle cap 2 are transparent plastics material.The body 1 is cylinder, institute The top and the realization of bottle cap 2 for stating body 1 are threadedly engaged.It can be completely embedded, guarantee sealed environment when culture in bottle.
Cylindrical cavity is set for the convenience of description, defining in the body 1, the bottom surface central location of the inner cavity is upward Vertical is equipped with inserting column 3, and the inserting column 3 is used to cooperate the associated component of functional module to realize that dismounting uses.It is inserted using outstanding Column 3, and not applicable recess slot realizes cooperation, mainly in view of to hold culture solution in culture bottle, and cultivates plant tissue, So this one small slot, is difficult to accomplish thoroughly to clean and sterilize, influences Reusability, also make troubles to maintenance.It is described to insert Column 3 is equipped with external screw thread.
2) functional module
The functional module includes the installation axle 5 being used in combination and isolation board 6, and be mounted on isolation board 6 can The mirror board 7 torn open, and the fastener 8 of fixed mirror board 7.
The bottom end of the installation axle 5 is equipped with the thread groove 9 matched with inserting column 3, and the thread groove 9 is matched with 3 screw thread of inserting column It closes, realizes that installation axle 5 is fixed relative to the installation in bottom of bottle center, it is convenient to disassembly, it is fixed reliable.
A plurality of stupefied muscle 10 is circumferentially distributed in the installation axle 5, the stupefied muscle 10 extends along the length direction of installation axle 5, Anti-skidding effect is played, is mainly used for cooperating with isolation board 6, improves the fixed effect of isolation board 6.
The isolation board 6 is the rectangle transparent plastic thin that width and 1 radius of body are adapted, the isolation board 6 with The adjacent side of installation axle 5 is defined as inside, and the isolation board 6 side adjacent with 1 inner wall of body is defined as outside.It is described every Inside from plate 6 is equipped with c-type axle sleeve 11, and the c-type axle sleeve 11 excessively cooperates with installation axle 5, and the radian of the c-type axle sleeve 11 is 250 degree.There is stupefied slot 12 along its radian directional spreding in the c-type axle sleeve 11, the stupefied slot 12 is used in combination with stupefied muscle 10.
By the structure of above-mentioned isolation board 6, the c-type axle sleeve 11 can removably cover in installation axle 5, and with It can be circumferentially rotated along installation axle 5 in the case of power, the rotational angle so as to adjust isolation board 6 with respect to installation axle 5.It realizes, Inner cavity is divided into two effects for arriving multiple fan-shaped regions.
The bottom of the isolation board 6 is equipped with the tooth socket 13 extended along its length, width of the tooth socket 13 along isolation board 6 Direction is spent with distribution, and the circulation of Easy-used bottle bottom culture substrate guarantees in different area of isolation the culture environment of bottom of bottle and support Slitting part is identical.
The outboard side edge of the isolation board 6 is provided along its length the arc-like sheet 14 being adapted with 1 inner wall radian of body, The arc-like sheet 14 is used to cooperate with the inner wall of body 1, and spacer is facilitated preferably to rotate and be isolated.
The height that the isolation board 6 can be relatively isolated plate 6 according to c-type axle sleeve 11 is different, is divided into 6 (c-type of I isolation board Axle sleeve 11 is on top), No. II isolation board 6 (1/4~1/2 position of the c-type axle sleeve 11 on top), No. III (the c-type axle sleeve of isolation board 6 11 lower part 1/2~1/4 position), be divided into No. IV isolation board 6 (c-type axle sleeve 11 is in bottom end), so as at most in a bottle Four spacers are set in body 1, body 1 is divided into four identical or different fan-shaped regions.
The isolation board 6 is transparent configuration, facilitates observation, is avoided because observing dead angle caused by isolation.Meanwhile laboratory Culture for plant tissue, due to being usually present observation problem in the lesser situation of plant.Namely for plant tissue Surface shape is difficult to see, and plant tissue can not be taken out bottle observation by the limitation based on culture, while also cannot By some common tools.Carry out very hang-up to record paper.Therefore the present apparatus is based on before to small plant Plant Tissue Breeding It puts, is also made that corresponding design particular for this problem, groove is set on the two sides side of isolation board 6, it is described recessed Slot is half through slot of top surface opening, facilitates installation.For placing mirror board 7 in the groove, the mirror board 7 is that thickness exists The metal plate of 0.8mm, surface setting have the mirror surface of enlarging function (curved surface is slightly handled), not according to amplification factor Together, 2 times, 5 times are set, and the mirror board 7 of two kinds of specifications is planted simultaneously when needing the shape to plant tissue clearly to be observed Object tissue is also smaller (leaf, root etc. are smaller, such as moss etc.), so that it may selective to open mirror board 7 from the top surface of groove Mouthful insertion bottom, the bottom of the groove are equipped with baffle 19, and the baffle 19 can realize limit and solid to the bottom side of mirror board 7 Fixed, the two sides of the groove are equipped with low water 20, and the fastener 8 is the frame section of rectangle, and the both ends of the fastener 8 are inserted into falling In slot 20, so that the mirror board 7 of two sides be waled.As shown in the picture.
Entire functional module, the design including installation axle 5, mirror board 7 and fastener 8 is detachable widgets, especially Facilitate laboratory room managing and use, laboratory is according to the needs of cultivated plant, when culture common size plant tissue, just only Using basic module, it is no different with common culture bottle use.When the plant tissue cultivated is smaller, so that it may according to feelings Condition uses required amount of spacer, so that body 1 is divided into 2,3,4 partial sector areas.When plant tissue very little, it is difficult to When observation, mirror board 7 is further increased, the convenient enlarging function by mirror surface observes the minutia of plant.
The present apparatus is particularly applicable in Plant Tissue Breeding, has advantage following prominent:
1) specific aim of Plant Tissue Breeding is greatly enhanced, improves the observation in culture to plant tissue, improve experiment Quality and effect.
2) laboratory consumptive material and space resources are reduced.It can by the management to widgets and flexibly use using the present apparatus To realize the culture to different characteristics plant tissue.It, can at double by subregion when in face of the plant tissue compared with plantlet Culture bottle quantity is reduced, significantly saves occupied space resources, device resource, energy etc., it may be said that be greatly saved The consumptive material in laboratory, energy consumption, significantly reduce toxigenic capacity.
3) have versatility, be equipped with this culture bottle, just do not have to be equipped with other culture bottles, it can be in various situations Plant tissue it is general, not only be easy to use but also facilitate management.
Embodiment 2: partition type plant tissue culture bottle.
Mainly there is different structures in terms of following two relative to 1 example of embodiment:
1) basic module is different from the fit structure of installation axle 5: this exemplary described inserting column 3 is polished rod structure, without spiral shell Line cooperates the socket 4 of 2 bottom surface of bottle cap, can be good at playing functional module fixed effect, and without screw thread convenient for cleaning. Stud position is corresponded on the bottom surface of the bottle cap 2 and is equipped with socket 4, and 3 position of inserting column is corresponded on the bottom surface of the socket 4 equipped with slotting Slot, so that installation axle 5 is fixed in upper and lower ends, it is same reliable and stable.And easy to process and cleaning.
2) mark function is increased:
The functional module further includes the component for label --- and marking plate 15, the marking plate 15 are plastic assembly, The side of the marking plate 15 be equipped with the first slotting axis 16 and the second slotting axis 17, the first slotting axis 16 it is adjacent with marking plate 15 one Side is parallel, and in the same plane with marking plate 15, and the second slotting axis 17 is parallel with the first slotting axis 16, and the second slotting axis 17 apart from 15 place plane of marking plate be 0.8cm.
The top side of the isolation board 6 is equipped with the annular element 18 with the first slotting 17 activity grafting of axis of slotting axis 16 or the second, works as institute When stating the insertion of the first slotting axis 16, marking plate 15 is open and flat as shown in the picture, and people is facilitated clearly to observe corresponding fan from top The plant tissue information in shape region;When the described second slotting axis 17 is inserted into annular element 18, marking plate 15 can be fallen naturally For vertical state, the plant tissue information of fan-shaped region where facilitating people corresponding from 1 side of body, and sector will not be blocked The upper space in region.
By above structure, the plant tissue information in each fan-shaped region can be identified.Facilitate researcher clear Chu, the corresponding informance in time, easily observing plant tissue in corresponding region.Simultaneously, it is contemplated that different plant tissues pair Whether the requirement of condition of culture is different, and need when top is observed, and different slotting axis is arranged, and realizes label The flattening of plate 15 or marking plate 15 vertical two states are installed and used, more flexible practical.
3) bottom side of the mirror board 7 is thin, top side is thick, and thickness difference is 1~2mm, so as to tilt down mirror surface, Shorter plant tissue observation is cooperated to use.

Claims (8)

1. partition type plant tissue culture bottle, which is characterized in that including basic module and functional module;
The basic module includes the body and bottle cap being used in combination, and the body is that section is circular straight cylinder;
The functional module includes installation axle, isolation board, mirror board, during the installation axle is detachably mounted to inside body Centre, the width of the isolation board and body inside radius are adapted, the dismountable company of rotation of the inside of the isolation board and installation axle It connects, the outside of the isolation board is in contact with body inner wall, is removably equipped with mirror surface on the two sides side of the isolation board Plate, the surface of the mirror board are equipped with the mirror surface with amplification factor, and the amplification factor is 2~8 times.
2. partition type plant tissue culture bottle according to claim 1, which is characterized in that the interior bottom surface center of the body Equipped with inserting column, the bottom end of the inserting column and installation axle is threadedly engaged.
3. partition type plant tissue culture bottle according to claim 1, which is characterized in that the bottom surface center of the body is equipped with Inserting column, the bottom surface center of the bottle cap are equipped with socket, the bottom end of the installation axle and inserting column activity grafting, the top of the installation axle End and socket activity grafting.
4. partition type plant tissue culture bottle according to claim 1, which is characterized in that the inboard of the isolation board is equipped with C Profile shaft set, the c-type axle sleeve and installation axle transition fit.
5. partition type plant tissue culture bottle according to claim 4, which is characterized in that the surface of the installation axle is along its axis To the stupefied muscle extended vertically is equipped with, the inner peripheral surface of the c-type axle sleeve is equipped with the stupefied slot being adapted with stupefied muscle.
6. partition type plant tissue culture bottle according to claim 4, which is characterized in that according to the c-type axle sleeve it is opposite every Isolation board is set different model by difference from plate height.
7. partition type plant tissue culture bottle according to claim 1, which is characterized in that the functional module further includes label The side of plate, the marking plate is equipped with the first slotting axis and the second slotting axis in parallel, and the first slotting axis and marking plate are same In one plane, vertical range of the second slotting wheelbase from the plane where marking plate is 0.5~1.5cm, the isolation board Top side is equipped with the annular element that cooperation can be inserted and rotated by the first slotting axis or the second slotting axis activity.
8. partition type plant tissue culture bottle according to claim 1, which is characterized in that the thickness of the mirror board top side is big Thickness in its bottom side, its mirror surface inclined surface after being mounted on the mirror board on isolation board.
CN201910274514.9A 2019-04-08 2019-04-08 Partition type plant tissue culture bottle Pending CN109874674A (en)

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Application Number Priority Date Filing Date Title
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021047096A1 (en) * 2019-09-11 2021-03-18 陈俊林 Anti-contamination mycobacterium tuberculosis culture tube

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CN209931157U (en) * 2019-04-08 2020-01-14 内江师范学院 Partition type plant tissue culture bottle
CN211002904U (en) * 2019-11-27 2020-07-14 攀枝花学院 Adjustable classification garbage can
CN215684010U (en) * 2021-09-03 2022-02-01 漳州绿林生物科技有限公司 Partition type plant tissue culture bottle
CN216499435U (en) * 2021-05-17 2022-05-13 南京昂科利医学检验实验室有限公司 Reagent bottle with detachable division board

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CN203538920U (en) * 2013-11-08 2014-04-16 陈治颖 Dressing mirror
CN104186231A (en) * 2014-08-01 2014-12-10 湖州盛基金属制品有限公司 Outdoor planting box
CN107946950A (en) * 2017-12-06 2018-04-20 江苏艮德电力设备有限公司 A kind of collapsible double-radiation function switchgear
CN208626225U (en) * 2018-03-08 2019-03-22 重庆和喜锦鸿医药包装有限公司 A kind of Multifunctional pharmaceutical bottle
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WO2021047096A1 (en) * 2019-09-11 2021-03-18 陈俊林 Anti-contamination mycobacterium tuberculosis culture tube

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