CN208191731U - Japanese Premna tissue culture flasks - Google Patents

Japanese Premna tissue culture flasks Download PDF

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Publication number
CN208191731U
CN208191731U CN201820406607.3U CN201820406607U CN208191731U CN 208191731 U CN208191731 U CN 208191731U CN 201820406607 U CN201820406607 U CN 201820406607U CN 208191731 U CN208191731 U CN 208191731U
Authority
CN
China
Prior art keywords
lid
japanese premna
transparent sleeve
tissue culture
culture flasks
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201820406607.3U
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Chinese (zh)
Inventor
陶阿丽
曹殿洁
杨春燕
白娟
李胜男
倪天明
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Anhui Xinhua University
Original Assignee
Anhui Xinhua University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Anhui Xinhua University filed Critical Anhui Xinhua University
Priority to CN201820406607.3U priority Critical patent/CN208191731U/en
Application granted granted Critical
Publication of CN208191731U publication Critical patent/CN208191731U/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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Abstract

The utility model relates to a kind of Japanese Premna tissue culture flasks, including the culture bottle for containing culture solution, the bottleneck of the culture bottle is removable to be provided with transparent sleeve, the tube port position of the transparent sleeve is provided with lid, multiple air holes are provided on the lid, breathable films are provided in the air hole, the edge of the lid and the nozzle of transparent sleeve constitute sealing cooperation, Japanese Premna tissue is placed in culture bottle, the bottle lid of culture bottle is set into transparent sleeve, light transmission sleeve upper cover sets lid, air hole is set on lid, breathable films are set in air hole, the film does not hinder normal air to pass through, miscellaneous bacteria can be also isolated in outside culture bottle, so as to effectively improve the success rate of Japanese Premna tissue cultivating.

Description

Japanese Premna tissue culture flasks
Technical field
The utility model relates to test apparatus technical fields, and in particular to a kind of Japanese Premna tissue culture flasks.
Background technique
Japanese Premna is distributed in China Yangtze river basin and areas to the south, and Japanese Premna itself has high medical value and food With value, premna microphylla itself is direct-edible, can also smash filtering to pieces and be fabricated to bavin leaf tofu, and not only nutritive value is high, and And mouthfeel is pleasant, cunning is tender.
To obtain ideal Japanese Premna culture data, Japanese Premna, which needs to be placed on, carries out isolation cultivation in culture vessel, training In feeding process, need individually to propose the tissue of Japanese Premna, and trained in the culture solution being placed in culture vessel It educates, existing culture vessel is mostly open bottle structure, constant temperature cultivation is carried out although can be placed on according to actual needs in incubator, However be difficult in the external fungi involvement culture solution of control, Japanese Premna tissue leads to Japanese Premna tissue often due to fungi turns over sets It rots, and then causes the cultivation success rate of Japanese Premna not high.
Utility model content
The purpose of this utility model is: a kind of Japanese Premna tissue culture flasks are provided, can effectively inhibit external fungi into Enter in culture bottle, improves the success rate of Japanese Premna tissue cultivating.
In order to achieve the above purposes, the technical solution adopted by the utility model is:
Japanese Premna tissue culture flasks, including the culture bottle for containing culture solution, the bottleneck of the culture bottle is removable to be set It is equipped with transparent sleeve, the tube port position of the transparent sleeve is provided with lid, and multiple air holes are provided on the lid, described Breathable films are provided in stomata, the edge of the lid and the nozzle of transparent sleeve constitute sealing cooperation.
The utility model is also equipped with following characteristics:
The nozzle inner wall of the transparent sleeve is provided with sealing ring, and the edge of the lid and the upper ring surface of sealing ring support It leans on.
The nozzle inner wall of the transparent sleeve is additionally provided with stop collar, and the stop collar is located at arrangement, institute above sealing ring It states and is arranged jagged on stop collar, the edge of the lid is provided with clamping joint.
The internal diameter of the sealing ring is less than the race diameter of stop collar.
The lid is provided centrally with rib, and the rib is in cross, and the edge of the rib is provided with annulus, institute The area of absence stated between rib and annulus constitutes air hole.
The bottleneck of the culture bottle is provided with thread segment and is threadedly coupled with one end of transparent sleeve composition.
The bottom of bottle of the culture bottle is provided with cross shelf.
The outer wall of the transparent sleeve is provided with ring positioning ring.
Compared with the prior art, the beneficial effects of the utility model are embodied in: Japanese Premna tissue is placed in culture bottle, The bottle lid of culture bottle is set into transparent sleeve, light transmission sleeve upper cover sets lid, and air hole, setting in air hole are arranged on lid Breathable films, the film do not hinder normal air to pass through, and also miscellaneous bacteria can be isolated in outside culture bottle, so as to effectively improve The success rate of Japanese Premna tissue cultivating.
Detailed description of the invention
Fig. 1 is the cross-sectional view of Japanese Premna tissue culture flasks;
Fig. 2 is the lid cross-sectional view of Japanese Premna tissue culture flasks;
Fig. 3 is the top view of Japanese Premna tissue culture flasks;
Fig. 4 is the top view of transparent sleeve;
Fig. 5 is the top view of culture bottle.
Specific embodiment
In conjunction with Fig. 1 to Fig. 5, the utility model is further described:
Japanese Premna tissue culture flasks, including the culture bottle 10 for containing culture solution, the bottleneck of the culture bottle 10 is removable Formula is provided with transparent sleeve 20, and the tube port position of the transparent sleeve 20 is provided with lid 30, is provided on the lid 30 multiple Air hole 31 is provided with breathable films 32, the edge of the lid 30 and the nozzle structure of transparent sleeve 20 in the air hole 31 Cooperate at sealing;
Referring to figs. 1 and 2, Japanese Premna tissue is placed in culture bottle 10, the bottle lid of culture bottle 10 is set Bright sleeve 20,20 upper cover of light transmission sleeve set lid 30, and air hole 31 is arranged on lid 30, breathable films are arranged in air hole 31 32, which does not hinder normal air to pass through, and can also be isolated in miscellaneous bacteria outside culture bottle 10, so as to effectively improve beans The success rate of rotten bavin tissue cultivating;
Above-mentioned transparent sleeve 20 may be provided on incubator or culturing rack, for cultivate the culture bottle 10 of Japanese Premna with Detachable connection is constituted between transparent sleeve 20, facilitates pick-and-place, and transparent sleeve 20 can effectively extend the growth of Japanese Premna Space;
Breathable films common in the market can be selected in breathable films 32, preferably selection transparent membrane, do not influence normal illumination.
As a preferred embodiment of the present invention, as shown in connection with fig. 1, have to improve lid 30 with what transparent sleeve 20 sealed Effect property, the nozzle inner wall of the transparent sleeve 20 are provided with sealing ring 21, the edge of the lid 30 and the upper ring of sealing ring 21 Face against.
It is further preferable that as shown in connection with fig. 4, the nozzle inner wall of the transparent sleeve 20 is additionally provided with stop collar 22, described Stop collar 22, which is located above sealing ring 21, to be arranged, jagged 221 is arranged on the stop collar 22, the edge setting of the lid 30 There is clamping joint 33;
When practical operation, it is aligned the clamping joint 33 of lid 30 with notch 221, then makes the edge of lid 30 Holding between stop collar 22 and sealing ring 21, and the edge of sealing ring 21 and lid 30 against, to realize sealing, barrier Miscellaneous bacteria.
It is further preferable that the internal diameter of the sealing ring 21 is less than the race diameter of stop collar 22.
As shown in connection with fig. 3, for convenience of lid 30 is disassembled from transparent sleeve 20, the center of the lid 30 is arranged Have a rib 34, the rib 34 is in cross, and the edge of the rib 34 is provided with annulus 35, the rib 34 and annulus 35 it Between area of absence constitute air hole 31.
Preferably, the bottleneck of the culture bottle 10 is provided with thread segment and is threadedly coupled with one end of transparent sleeve 20 composition.
As shown in connection with fig. 5, the bottom of bottle of the culture bottle 10 is provided with cross shelf 11;
Above-mentioned cross shelf 11 constitutes the culture chamber for individually cultivating Japanese Premna tissue, improves and cultivates efficiency.
For convenience of the location and installation realized to transparent sleeve 20, the outer wall of the transparent sleeve 20 is provided with ring positioning ring 23。
It is obvious to a person skilled in the art that the present invention is not limited to the details of the above exemplary embodiments, and And without departing substantially from the spirit or essential attributes of the utility model, it can realize that this is practical new in other specific forms Type.Therefore, in all respects, the present embodiments are to be considered as illustrative and not restrictive, this is practical new The range of type is indicated by the appended claims rather than the foregoing description, it is intended that containing for the equivalent requirements of the claims will be fallen in All changes in justice and range are embraced therein.It should not treat any reference in the claims as limiting Related claim.
In addition, it should be understood that although this specification is described in terms of embodiments, but not each embodiment is only wrapped Containing an independent technical solution, this description of the specification is merely for the sake of clarity, and those skilled in the art should It considers the specification as a whole, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art The other embodiments being understood that.

Claims (8)

1. Japanese Premna tissue culture flasks, it is characterised in that: including the culture bottle (10) for containing culture solution, the culture bottle (10) bottleneck is removable to be provided with transparent sleeve (20), and the tube port position of the transparent sleeve (20) is provided with lid (30), institute It states and is provided on lid (30) multiple air holes (31), be provided with breathable films (32), the lid in the air hole (31) (30) nozzle at edge and transparent sleeve (20) constitutes sealing cooperation.
2. Japanese Premna tissue culture flasks according to claim 1, it is characterised in that: in the nozzle of the transparent sleeve (20) Wall is provided with sealing ring (21), the upper ring surfaces of the edge of the lid (30) and sealing ring (21) against.
3. Japanese Premna tissue culture flasks according to claim 2, it is characterised in that: in the nozzle of the transparent sleeve (20) Wall is additionally provided with stop collar (22), and the stop collar (22) is located at arrangement above sealing ring (21), is set on the stop collar (22) It is equipped with notch (221), the edge of the lid (30) is provided with clamping joint (33).
4. Japanese Premna tissue culture flasks according to claim 3, it is characterised in that: the internal diameter of the sealing ring (21) is less than The race diameter of stop collar (22).
5. Japanese Premna tissue culture flasks according to claim 4, it is characterised in that: lid (30) are provided centrally with Rib (34), the rib (34) are in cross, and the edge of the rib (34) is provided with annulus (35), the rib (34) with Area of absence between annulus (35) constitutes air hole (31).
6. Japanese Premna tissue culture flasks according to claim 1, it is characterised in that: the bottleneck of the culture bottle (10) is arranged There is thread segment to constitute with one end of transparent sleeve (20) to be threadedly coupled.
7. Japanese Premna tissue culture flasks according to claim 6, it is characterised in that: the bottom of bottle of the culture bottle (10) is arranged There are cross shelf (11).
8. Japanese Premna tissue culture flasks according to claim 7, it is characterised in that: the outer wall of the transparent sleeve (20) is set It is equipped with ring positioning ring (23).
CN201820406607.3U 2018-03-23 2018-03-23 Japanese Premna tissue culture flasks Expired - Fee Related CN208191731U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201820406607.3U CN208191731U (en) 2018-03-23 2018-03-23 Japanese Premna tissue culture flasks

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201820406607.3U CN208191731U (en) 2018-03-23 2018-03-23 Japanese Premna tissue culture flasks

Publications (1)

Publication Number Publication Date
CN208191731U true CN208191731U (en) 2018-12-07

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ID=64527600

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201820406607.3U Expired - Fee Related CN208191731U (en) 2018-03-23 2018-03-23 Japanese Premna tissue culture flasks

Country Status (1)

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CN (1) CN208191731U (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109874674A (en) * 2019-04-08 2019-06-14 内江师范学院 Partition type plant tissue culture bottle
CN111387056A (en) * 2020-04-02 2020-07-10 贵州大学 Method for establishing tissue culture system for premna hirta

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109874674A (en) * 2019-04-08 2019-06-14 内江师范学院 Partition type plant tissue culture bottle
CN111387056A (en) * 2020-04-02 2020-07-10 贵州大学 Method for establishing tissue culture system for premna hirta
CN111387056B (en) * 2020-04-02 2021-10-22 贵州大学 Method for establishing tissue culture system for premna hirta

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GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20181207

Termination date: 20200323

CF01 Termination of patent right due to non-payment of annual fee