CN104764639A - Soil microorganism fumigation apparatus and application thereof - Google Patents
Soil microorganism fumigation apparatus and application thereof Download PDFInfo
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- CN104764639A CN104764639A CN201510120199.6A CN201510120199A CN104764639A CN 104764639 A CN104764639 A CN 104764639A CN 201510120199 A CN201510120199 A CN 201510120199A CN 104764639 A CN104764639 A CN 104764639A
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Abstract
The invention discloses a soil microorganism fumigation apparatus and an application thereof. A fumigation apparatus body is covered by a shading outer cover and includes a fumigation sealed chamber, a fumigation soil sample cup body apparatus, a temperature control assembly and a fumigation heating assembly. The fumigation apparatus body is communicated with a vacuum pump through a gas pipeline to form the system apparatus. Compared with conventional methods and devices, the soil microorganism fumigation apparatus is ensured in air impermeability; the heating and heat-insulating apparatus can ensure a temperature controlling requirement in fumigation cultivation, so that an operation of placing the fumigation apparatus in a emperature-constant cultivation box is omitted; and the shading outer cover can ensure a dark-light cultivation condition, so that an operation of placing the fumigation apparatus in a dark box is omitted, thereby reducing labor intensity and cost, improving the efficiency of the fumigation cultivation of soil microorganisms and simplifying a cultivation process. By means of proper selection of the number of the fumigation heating assemblies, through an active fumigation process, the contact area of soil to chloroform is fully increased and steam flux is increased, so that the efficiency of the fumigation is increased and the time of the fumigation is reduced.
Description
Technical field
The invention belongs to soil microorganism detection technique field, concretely, the present invention relates to the technical field of Soil Microorganism carbon, nitrogen analysis, more specifically, the present invention relates to a kind of technical field of soil microbe fumigating device.
Background technology
Existing soil microbe fumigating device mostly is glass material, a ceramic wafer with holes is placed with in the middle part of base, can take out, on be stamped piston (valve), for controlling the opening of container, during use, soil is loaded small beaker, put into exsiccator, vaseline airtight container is smeared at the exsiccator place of combining between the higher and lower levels, and opens upper valve connection vacuum pump and bleeds, closure piston and vacuum pump after chloroform boiling.
Known through retrieval prior art Patent Literature, denomination of invention is that (patent No. is ZL201110360143X to soil microbe fumigating device, patent publication No. is CN 102507286 B) and a kind of soil microbe fumigating device (patent No. is ZL 2011204488406, patent announcement number is CN 202362179 U) a kind of soil microbe fumigating device is disclosed, adopt the chloroform vapor in the container of the base of accommodating cavity can only could arrive gas outlet by pedotheque along with draft, the external world is discharged to by gas piping, a container is provided with in the base of accommodating cavity described in this device, with how many enforcements of fumigating soil sample can not fumigate fully, also the stifling time cannot be shortened, make efficiency stifling not high, simultaneously, fumigate in research and production actual soil microorganism, need after bleeding device to be moved in temperature control, lucifuge equipment and cultivate, consume human and material resources resource, the container environment providing equipment to hold stifling material does not have the water of uniform temperature, have impact on the initial temperature requirement that chloroform fumigates process, these are all the deficiencies in the prior art, need badly and are improved.
Summary of the invention
For prior art present situation and Problems existing, a kind of soil microbe fumigating device provided by the invention, its lid is connected with cavity to be fixed by clamp web member, ensure that the impermeability of device for fumigation; Heating constant-temperature equipment ensure that stifling temperature control requirement of cultivating, and eliminates the operation of in the past device for fumigation being put into constant incubator; Light-proof outer cover ensure that half-light condition of culture, eliminates operation device for fumigation moved into camera bellows in the past and cultivate, decreases manpower and materials, improve soil microorganism and fumigate culture efficiency, simplify cultivation process, be with a wide range of applications.
It is adopt following technical scheme to realize that the present invention solves its technical matters:
A kind of soil microbe fumigating device provided by the invention covers on stifling agent set by light-proof outer cover, stifling agent set comprises stifling seal chamber, fumigating soil sample cup body device, temperature-controlling component, stifling heating component composition, and stifling agent set to be connected with vacuum pump composition system and device by gas piping.Compare with equipment with classic method, ensure that the impermeability of device for fumigation; Heating constant-temperature equipment ensure that stifling temperature control requirement of cultivating, and eliminates the operation of in the past device for fumigation being put into constant incubator; Light-proof outer cover ensure that half-light condition of culture, eliminates operation device for fumigation moved into camera bellows in the past and cultivate, decreases manpower and materials, improve soil microorganism and fumigate culture efficiency, simplify cultivation process; The quantity of heating component is fumigated in good time selection, adopts initiatively fumigation technology, increases the contact area of soil and chloroform fully, add vapor flux, thus can improve stifling efficiency and shorten fumigation time.
Soil microbe fumigating device provided by the invention, in stifling agent set, stifling seal chamber is made up of lid, stifling cavity and piston; Lid matches by pressing with stifling cavity, then fixes with clamp web member; The tops of lid arranges piston, and tops is provided with gas piping, is connected with the vacuum pump that the external world is arranged by gas piping, and vacuum pump is used for vacuumizing stifling seal chamber; The vacuum tightness of piston for controlling that gas piping is communicated with extraneous, in closedown and stifling seal chamber; The inwall of stifling cavity is provided with projection, every one deck projection is uniformly distributed, projection shelves dividing plate, dividing plate is provided with and is evenly arranged and equal-sized circular hole, dividing plate is placed with fumigating soil sample cup body device, fumigating soil sample cup body device and stifling cavity and dividing plate form samples-soil and fumigate environment; Be provided with stifling heating component in the bottom of stifling cavity, this stifling heating component is the collar shape heating arrangement of built-in stifling heating component cavity volume, and stifling heating component cavity volume is used for holding stifling material, and common stifling material adopts chloroform.
In soil microbe fumigating device provided by the invention, there is the hot water of uniform temperature in the cavity of stifling heating component, for heating or humid control the stifling material in stifling heating component cavity volume.
In soil microbe fumigating device provided by the invention, lid by with stifling cavity junction can adopt smear vaseline after press and match, then clamp fixing with clamp web member, also coordinate by alternate manners such as transition fit.
In soil microbe fumigating device provided by the invention, the inwall of stifling cavity is provided with multi-layer bumps, every one deck projection is uniformly distributed, and between each layer projection, the radial preferential angle adopted of being separated by is 30 degree, ensure that and dividing plate is conveniently placed with fumigating soil sample cup body device and shelves dividing plate.
In soil microbe fumigating device provided by the invention, stifling cavity inner wall is provided with temperature regulating device, is connected with power supply, for controlling stifling cavity inner temperature.
In soil microbe fumigating device provided by the invention, fumigation chamber is external establishes a taper light-proof outer cover, light-proof outer cover adopts detachable or adopts type push form, adopt light-proof outer cover to be convenient to controlled light and observe stifling state of matter, ensure that half-light condition of culture, eliminate operation device for fumigation moved into camera bellows in the past and cultivate.
In soil microbe fumigating device provided by the invention, in above-mentioned soil microbe fumigating device, described gas piping can be provided with tensimeter and corresponding individual event valve, be respectively used to control gas piping and be connected with the vacuum pump in the external world and close and vacuum tightness in stifling seal chamber.
Soil microbe fumigating device provided by the invention, adopt initiatively fumigation technology, namely the chloroform vapor of the built-in stifling heating component cavity volume of the stifling heating component of the base of accommodating cavity can only could arrive gas outlet by pedotheque along with draft, is discharged to the external world by gas piping; Compare with classic method the contact area increasing soil and chloroform with equipment, add vapor flux, thus stifling efficiency can be improved and shorten fumigation time.
Simultaneously, the invention provides the application of above-mentioned soil microbe fumigating device in soil microorganism detects, fumigation technology initiatively can be adopted, first stifling material is added in built-in in stifling heating component stifling heating component cavity volume, modal stifling material adopts chloroform, then pedotheque is loaded in fumigating soil sample cup body device, compress lid, with clamp web member, lid is connected with stifling cavity, by piston, gas piping is connected with vacuum pump, open vacuum pump to bleed, after stifling material seethes with excitement or burns, gas is full of the pedotheque in stifling seal chamber and fumigating soil sample cup body device, stifling material boiling or burning be closure piston and vacuum pump after 5 minutes, switch on power, open temperature regulating device, regulate temperature, then the upper light-proof outer cover of cover, carry out 24 hours stifling cultivations to process, after end, take off light-proof outer cover, close temperature regulating device and power supply, this device for fumigation gas piping is connected with vacuum pump, opens piston and vacuum pump, repeatedly bleed until material stifling in soil is taken out to the greatest extent.
According to actual needs, above-mentioned soil microbe fumigating device can be made further optimization and/or improvements and is further described:
In the present invention, in above-mentioned soil microbe fumigating device, described dividing plate can be stainless steel mesh screen, ceramic material or tempered glass, is convenient to the boiling situation of observing chloroform in stifling process.
In the present invention, the air hole arranged in stifling cavity median septum is for circular, and be uniformly distributed, circular hole hole number and size all can adjust according to actual needs.
In the present invention, in above-mentioned soil microbe fumigating device, the material of described stifling seal chamber, fumigating soil sample cup body device and stifling heating component lid and accommodating cavity can be stainless steel or teflon, also may be selected to be other corrosion-resistant material.
In the present invention, the projection of stifling cavity inner wall can place two-layer or multilayer, experimentally can need to place multiple dividing plate, realize making full use of of chamber intracorporeal space.
In the present invention, light-proof outer cover material can select PU to remove from office material.
The beneficial effect that the present invention reaches:
(1) in soil microbe fumigating device provided by the invention, lid by with stifling cavity junction adopt smear vaseline after press other transition fit such as matching, adopt clamp web member to fix mode coordinate, ensure that the impermeability of device for fumigation.
(2) in soil microbe fumigating device provided by the invention, the hot water of uniform temperature is held in the cavity volume of the built-in stifling heating component cavity volume periphery of stifling heating component, by the temperature control action of temperature regulating device, ensure that stifling temperature control requirement of cultivating, eliminate the operation of in the past device for fumigation being put into constant incubator, improve the efficiency that soil microorganism is stifling.
(3) in soil microbe fumigating device provided by the invention, by the detachable of stifling agent set peripheral hardware one taper or adopt type push form light-proof outer cover, can advantageously controlled light intensity and be convenient to observe stifling state of matter, ensure that half-light condition of culture, eliminate operation device for fumigation moved into camera bellows in the past and cultivate.
(4) be suitable in soil microbe fumigating device provided by the invention, can according to the number of fumigating soil sample, the quantity of heating component is fumigated in good time selection, adopt initiatively fumigation technology, ensure that the chloroform vapor of the built-in stifling heating component cavity volume of the stifling heating component of the base of accommodating cavity evenly can arrive gas outlet along with draft by pedotheque, be discharged to the external world by gas piping; Compare with classic method the contact area increasing soil and chloroform fully with equipment, add vapor flux, thus stifling efficiency can be improved and shorten fumigation time.
Accompanying drawing explanation
Fig. 1 is shown as a kind of one-piece construction schematic diagram of soil microbe fumigating device.
Fig. 2 is shown as a kind of protruding vertical view of soil microbe fumigating device.
Fig. 3 is shown as a kind of stifling heating component schematic diagram of soil microbe fumigating device.
Fig. 4 is shown as a kind of stifling heating component cut-open view of soil microbe fumigating device.
In Fig. 1-4: 1-lid, 2-piston, 3-fumigate cavity, 4-gas piping, 5-dividing plate, 6-circular hole, 7-fumigate heating component, 8-lower floor projection, 9-temperature regulating device, 10-clamp web member, 11-vacuum pump, 12-light-proof outer cover, 13-power supply, 14-fumigating soil sample cup body device (14), 15-middle level are protruding, the protruding 17-in 16-upper strata fumigates heating component cavity volume, 18-hot water, 19-R, between each layer projection, radial direction is separated by 30 degree.
Embodiment
Below, 1-4 and embodiment, be described in further detail the present invention by reference to the accompanying drawings.
In the present invention, for convenience of description, a kind of description of relative position relation of soil microbe fumigating device is that the Butut mode of 1-4 is with reference to the accompanying drawings described, as: the position relationship of upper and lower, left and right etc. determines according to the Butut direction of Figure of description.
See accompanying drawing 1-4, a kind of soil microbe fumigating device provided by the invention covers on stifling agent set by light-proof outer cover (12), stifling agent set comprises stifling seal chamber, fumigating soil sample cup body device (14), temperature-controlling component (9), stifling heating component (7) composition, and stifling agent set to be connected with vacuum pump (11) composition system and device by gas piping (4).
Soil microbe fumigating device provided by the invention, in stifling agent set, stifling seal chamber is made up of lid (1), stifling cavity (3) and piston (2); Lid (1) matches by pressing with stifling cavity (3), then uses clamp web member (10) to fix; The tops of lid (1) arranges piston (2), and tops is provided with gas piping (4), and be connected with the vacuum pump (11) that the external world is arranged by gas piping (4), vacuum pump (11) is for vacuumizing stifling seal chamber; The vacuum tightness of piston (2) for controlling that gas piping (4) is communicated with extraneous, in closedown and stifling seal chamber; The inwall of stifling cavity (3) is provided with projection (8,15,16), every one deck projection (8,15,16) is uniformly distributed, protruding (8,15,16) shelve dividing plate (5), dividing plate (5) is provided with and is evenly arranged and equal-sized circular hole (6), dividing plate (5) is placed with fumigating soil sample cup body device (14), fumigating soil sample cup body device (14) forms samples-soil with stifling cavity (3) and dividing plate (5) and fumigates environment; Stifling heating component (7) is provided with in the bottom of stifling cavity (3), this stifling heating component (7) is the collar shape heating arrangement of built-in stifling heating component cavity volume (17), stifling heating component cavity volume (17) is for holding stifling material, and common stifling material adopts chloroform.
In soil microbe fumigating device provided by the invention, there is the hot water (18) of uniform temperature in the cavity of stifling heating component (7), for heating or humid control the stifling material in stifling heating component cavity volume (17).
In soil microbe fumigating device provided by the invention, lid (1) by with stifling cavity (3) junction can adopt smear vaseline after press and match, then use clamp web member (10) to clamp fixing, also coordinate by alternate manners such as transition fit.
In soil microbe fumigating device provided by the invention, the inwall of stifling cavity (3) is provided with multi-layer bumps (8,15,16), every one deck projection (8,15,16) is uniformly distributed, and between each layer projection (8,15,16), the radial preferential angle R adopted of being separated by is 30 degree (19).
In soil microbe fumigating device provided by the invention, stifling cavity (3) inwall is provided with temperature regulating device (9), is connected, for controlling stifling cavity (3) interior temperature with power supply (13).
In soil microbe fumigating device provided by the invention, stifling cavity (3) peripheral hardware one taper light-proof outer cover (12), light-proof outer cover (12) adopts detachable or adopts type push form, adopt light-proof outer cover to be convenient to controlled light and observe stifling state of matter, ensure that half-light condition of culture, eliminate operation device for fumigation moved into camera bellows in the past and cultivate.
In soil microbe fumigating device provided by the invention, in above-mentioned soil microbe fumigating device, described gas piping (4) can be provided with tensimeter and corresponding individual event valve, be respectively used to control gas piping (4) and be connected with the vacuum pump (11) in the external world and close and vacuum tightness in stifling seal chamber.
Soil microbe fumigating device provided by the invention, adopt initiatively fumigation technology, namely the chloroform vapor of the built-in stifling heating component cavity volume (17) of the stifling heating component (7) of the base of accommodating cavity can only could arrive gas outlet by pedotheque along with draft, is discharged to the external world by gas piping; Compare with classic method the contact area increasing soil and chloroform with equipment, add vapor flux, thus stifling efficiency can be improved and shorten fumigation time.
Simultaneously, the invention provides the application of above-mentioned soil microbe fumigating device in soil microorganism detects, adopt fumigation technology initiatively, first stifling material is added in built-in in stifling heating component (7) stifling heating component cavity volume (17), modal stifling material adopts chloroform, then pedotheque is loaded in fumigating soil sample cup body device (14), compress lid (1), with clamp web member (10), lid (1) is connected with stifling cavity (3), by piston (2), gas piping (4) is connected with vacuum pump (11), open vacuum pump (11) to bleed, after stifling material seethes with excitement or burns, gas is full of the pedotheque in stifling seal chamber and fumigating soil sample cup body device (14), stifling material boiling or burning be closure piston (2) and vacuum pump (11) after 5 minutes, switch on power (13), open temperature regulating device (9), regulate temperature, then the upper light-proof outer cover (12) of cover, carry out 24 hours stifling cultivations to process, after end, take off light-proof outer cover (12), close temperature regulating device (9) and power supply (13), this device for fumigation gas piping (4) is connected with vacuum pump (11), open piston (2) and vacuum pump (11), repeatedly bleed until material stifling in soil is taken out to the greatest extent.
Simultaneously, the hot water (18) of uniform temperature is held in the cavity volume of built-in stifling heating component cavity volume (17) periphery of stifling heating component (7), by the temperature control action of temperature regulating device, ensure that stifling temperature control requirement of cultivating, eliminate the operation of in the past device for fumigation being put into constant incubator, improve the efficiency that soil microorganism is stifling.
According to actual needs, above-mentioned soil microbe fumigating device can be made further optimization and/or improvements and is further described:
In the present invention, in above-mentioned soil microbe fumigating device, described dividing plate (5) can be stainless steel mesh screen, ceramic material or tempered glass, is convenient to the boiling situation of observing chloroform in stifling process.
In the present invention, the air hole arranged in stifling cavity (3) median septum (5) is circular, and be uniformly distributed, circular hole (6) hole number and size all can adjust according to actual needs.
In the present invention, in above-mentioned soil microbe fumigating device, the material of described stifling seal chamber, fumigating soil sample cup body device (14) and stifling heating component (7) lid and accommodating cavity can be stainless steel or teflon, also may be selected to be other corrosion-resistant material.
In the present invention, the projection (8,15,16) of stifling cavity (3) inwall can place two-layer or multilayer, experimentally can need to place multiple dividing plate (5), realize making full use of of chamber intracorporeal space.
In the present invention, light-proof outer cover (12) material can select PU to remove from office material.
As mentioned above; the present invention can be realized preferably; the above embodiments are only be described the preferred embodiment of the present invention; not scope of the present invention is limited; under not departing from the present invention and designing the prerequisite of spirit; the various distortion that those of ordinary skill in the art make technical scheme of the present invention and improvement, all should fall in protection domain that the present invention determines.
Claims (10)
1. a soil microbe fumigating device, cover on stifling agent set by light-proof outer cover, stifling agent set to be connected with vacuum pump composition system and device by gas piping, it is characterized in that, stifling agent set comprises stifling seal chamber, fumigating soil sample cup body device, temperature-controlling component, stifling heating component composition.
2. soil microbe fumigating device as claimed in claim 1, is characterized in that, in described stifling agent set, stifling seal chamber is made up of lid, stifling cavity and piston; Lid matches by pressing with stifling cavity, then fixes with clamp web member; The tops of lid arranges piston, and tops is provided with gas piping, is connected with the vacuum pump that the external world is arranged by gas piping, and vacuum pump is used for vacuumizing stifling seal chamber; The vacuum tightness of piston for controlling that gas piping is communicated with extraneous, in closedown and stifling seal chamber; Be provided with stifling heating component in the bottom of stifling cavity, this stifling heating component is the collar shape heating arrangement of built-in stifling heating component cavity volume.
3. soil microbe fumigating device as claimed in claim 1, is characterized in that, described lid by with stifling cavity junction can adopt smear vaseline after press and match, then clamp fixing with clamp web member.
4. soil microbe fumigating device as claimed in claim 1, it is characterized in that, the inwall of described stifling cavity is provided with multi-layer bumps, and every one deck projection is uniformly distributed, and between each layer projection, radial direction is separated by and is preferentially adopted 30 degree.
5. soil microbe fumigating device as claimed in claim 1, it is characterized in that, described stifling cavity inner wall is provided with temperature regulating device, is connected with power supply, for controlling stifling cavity inner temperature.
6. soil microbe fumigating device as claimed in claim 1, it is characterized in that, described fumigation chamber is external establishes a taper light-proof outer cover, and light-proof outer cover adopts detachable or adopts type push form.
7. soil microbe fumigating device as claimed in claim 1, is characterized in that, in described soil microbe fumigating device, described gas piping can be provided with tensimeter and corresponding individual event valve.
8. soil microbe fumigating device as claimed in claim 1, is characterized in that, the air hole arranged in described stifling cavity median septum is for circular, and be uniformly distributed, circular hole hole number and size all can adjust according to actual needs.
9. soil microbe fumigating device as claimed in claim 1, it is characterized in that, the projection of described stifling cavity inner wall can place two-layer or multilayer, experimentally can need to place multiple dividing plate, realize making full use of of chamber intracorporeal space.
10. soil microbe fumigating device as claimed in claim 1, it is characterized in that, the inwall of described stifling cavity is provided with projection, every one deck projection is uniformly distributed, projection shelves dividing plate, dividing plate is provided with and is evenly arranged and equal-sized circular hole, dividing plate is placed with fumigating soil sample cup body device.
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| CN201510120199.6A CN104764639B (en) | 2015-03-19 | 2015-03-19 | A kind of soil microbe fumigating device and its application |
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| CN201510120199.6A CN104764639B (en) | 2015-03-19 | 2015-03-19 | A kind of soil microbe fumigating device and its application |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105277404A (en) * | 2015-10-08 | 2016-01-27 | 浙江省农业科学院 | Chloroform fumigation device for measuring soil microbial biomass carbon nitrogen and fumigation method |
| CN111751185A (en) * | 2020-07-07 | 2020-10-09 | 盐城工学院 | Soil microorganism fumigation device |
| CN112567916A (en) * | 2021-01-07 | 2021-03-30 | 郑祥瑞 | Reagent method fumigation system for soil improvement |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2043501U (en) * | 1989-01-20 | 1989-08-30 | 涂文林 | Apparatus for treating diseases with steam generated by boiling medicinal herbs |
| KR0181712B1 (en) * | 1990-05-26 | 1999-03-20 | 호르스트 슐츠 | Cooking pot with improved locking structure |
| CN102493429B (en) * | 2011-11-14 | 2014-12-17 | 曹传松 | Curing protection method of archaeological excavation site |
| CN102507286B (en) * | 2011-11-14 | 2014-06-25 | 中国科学院植物研究所 | Soil microbe fumigating device |
| CN103808544B (en) * | 2014-02-19 | 2016-03-16 | 中国科学院植物研究所 | A kind of easily extensible soil microbe fumigating device |
| CN204612995U (en) * | 2015-03-19 | 2015-09-02 | 新疆农业科学院土壤肥料与农业节水研究所 | A kind of soil microbe fumigating device |
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2015
- 2015-03-19 CN CN201510120199.6A patent/CN104764639B/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105277404A (en) * | 2015-10-08 | 2016-01-27 | 浙江省农业科学院 | Chloroform fumigation device for measuring soil microbial biomass carbon nitrogen and fumigation method |
| CN111751185A (en) * | 2020-07-07 | 2020-10-09 | 盐城工学院 | Soil microorganism fumigation device |
| CN112567916A (en) * | 2021-01-07 | 2021-03-30 | 郑祥瑞 | Reagent method fumigation system for soil improvement |
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