CN209065917U - A kind of device screening rhizobium - Google Patents
A kind of device screening rhizobium Download PDFInfo
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- CN209065917U CN209065917U CN201821758900.2U CN201821758900U CN209065917U CN 209065917 U CN209065917 U CN 209065917U CN 201821758900 U CN201821758900 U CN 201821758900U CN 209065917 U CN209065917 U CN 209065917U
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- fixedly connected
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- rhizobium
- screen box
- secondary screening
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Abstract
The utility model discloses a kind of devices for screening rhizobium, are related to agriculture technical field of microbe application.The utility model includes constant-temperature table: constant-temperature table output face is fixedly connected with just screen box；First screen box inner surface slidably connects waste liquid drawer；Filter plate is fixedly connected between first screen box inner surface；Filter plate is located at right above waste liquid drawer；One surface of filter plate is fixedly connected with beaker frame；Beaker frame inner wall is connected with culture medium；Constant-temperature table two sides are fixedly connected to bracket；One surface of bracket is fixedly connected with secondary screening case；Secondary screening case inner surface offers air hole；One surface of secondary screening case is fixedly connected with several weighing sensors.The utility model can filter out drought-enduring rihizobium japonicum on the one hand by comparative experiments method and quantitative analysis method by the design of first screen box, secondary screening case, culture medium and soil moisture humidity sensor；On the other hand, by multiple contrast verification, it can be improved the accuracy of drought-enduring rhizobium screening.
Description
Technical field
The utility model belongs to agriculture technical field of microbe application, more particularly to a kind of device for screening rhizobium.
Background technique
Biological nitrogen fixation is one of important source of soybean nitrogen, in Soybean production, is filled by being inoculated with excellent rhizobium
Its nitrogen fixing capacity is waved in distribution, is provided more nitrogen nutrition for soybean growth, is reduced chemical nitrogen fertilizer application, improve soybean yields and
Quality is the effective way for realizing cost-saving synergistic；
However, the China as Original area of soybean, the rate of vaccination of rihizobium japonicum is far below the U.S., Brazil, Argentina etc.
Soybean main production country；In the northwest soybean producing region of arid, the application of rhizobium is less, the main reason is that drought impact rhizobium
Dross and fixed nitrogen；Rhizobium are active and its very sensitive to drought environment with legume symbiosis, on the one hand soil water shortage makes
The breeding of rhizobium is restricted, it is difficult to legume symbiosis dross；On the other hand the growth for also influencing Soybean Root Hairs, reduces
The chance that rhizobium are infected, and then influence the foundation of the Symbiotic association；Studies have shown that passing through root nodule under drought condition
The intracorporal glycine betaine of plant, superoxide dismutase equal size can be improved in the nodulation and nitrogen fixation of bacterium, to enhance the drought resisting of soybean
Ability；Therefore, drought-enduring rihizobium japonicum is screened, improving soybean nodulation nitrogen fixing capacity under drought condition is particularly important.
Utility model content
The purpose of this utility model is to provide a kind of devices for screening rhizobium, pass through first screen box, secondary screening case, the soil water
The design for dividing humidity sensor, weighing sensor, nursery seat and culture medium, solves and lacks a kind of special drought resisting root on the market
The problem of tumor bacterium screening plant.
In order to solve the above technical problems, the utility model is achieved through the following technical solutions:
The utility model is a kind of device for screening rhizobium, including constant-temperature table:
The constant-temperature table output face is fixedly connected with just screen box；The just screen box inner surface slidably connects waste liquid pumping
Drawer；Filter plate is fixedly connected between the just screen box inner surface；The filter plate is located at right above waste liquid drawer；One table of filter plate
Face is fixedly connected with beaker frame；The beaker frame inner wall is connected with culture medium；
The constant-temperature table two sides are fixedly connected to bracket；One surface of bracket is fixedly connected with secondary screening case；Institute
It states secondary screening case inner surface and offers air hole；One surface of secondary screening case is fixedly connected there are four weighing sensor；Described in four
One surface of weighing sensor is fixedly connected to nursery seat；It is wet that four one surfaces of nursery seat are fixedly connected to soil moisture
Spend sensor.
Further, plant growth lamp is fixedly connected at the top of the secondary screening case inner surface；The secondary screening case is close to just
One surface of screen box has been respectively fixedly connected with Temperature Humidity Sensor and ultraviolet disinfection lamp.
Further, one surface of constant-temperature table is fixedly connected with control panel；Fixation is set inside the constant-temperature table
It is equipped with compressor, motor and eccentric wheel；The constant-temperature table is rotational oscillation formula.
Further, adjacent two surface of the bracket is fixedly connected to glass panel；One surface of glass panel is set
It is equipped with chamber door.
Further, four weighing sensors are in the linear array distribution in one surface of secondary screening case.
Further, one surface of filter plate offers filter opening；Nine beaker frame inner walls are fixedly connected to buffering
Rubber mat.
The device that the use of the new type provides should use in dark situation.
The utility model has the following beneficial effects:
1, the utility model passes through first screen box, secondary screening case, culture medium, weighing sensor and soil moisture humidity sensor
Design can filter out drought-enduring rihizobium japonicum on the one hand by comparative experiments method and quantitative analysis method；On the other hand, pass through
Multiple contrast verification can be improved the accuracy of drought-enduring rhizobium screening.
2, the utility model makes the screening plant by constant-temperature table, greenhouse degree sensor, soil moisture humidity sensor
It can be accurately controlled plant growth environment, guarantee the uniformity of data in experimentation, pass through weighing sensor and the soil water
Divide humidity sensor, enable the device to real-time monitoring and control the variable data in experimentation, guarantees that experimental data is final
Accuracy.
3, on the one hand the utility model can guarantee the nothing in screening process by ultraviolet disinfection lamp and plant growth lamp
Collarium border；On the other hand it can promote plant growth；Quickly culture medium can be vibrated by filter plate, filter opening and waste liquid drawer
The liquid splashed in journey is collected.
Certainly, any product for implementing the utility model does not necessarily require achieving all the advantages described above at the same time.
Detailed description of the invention
In order to illustrate more clearly of the technical solution of the utility model embodiment, make required for being described below to embodiment
Attached drawing is briefly described, it should be apparent that, the drawings in the following description are merely some embodiments of the present invention,
For those of ordinary skill in the art, without creative efforts, it can also be obtained according to these attached drawings
Other attached drawings.
Fig. 1 is a kind of structural schematic diagram of device for screening rhizobium；
Fig. 2 is the positive structure diagram of Fig. 1；
Fig. 3 is the side structure schematic view of Fig. 1；
In attached drawing, parts list represented by the reference numerals are as follows:
1- constant-temperature table, the first screen box of 2-, 3- waste liquid drawer, 4- filter plate, 5- beaker frame, 6- culture medium, 7- bracket, 8- secondary screening
Case, 9- air hole, 10- weighing sensor, 11- nursery seat, 12- soil moisture humidity sensor, 13- plant growth lamp, 14- temperature
Humidity sensor, 15- ultraviolet disinfection lamp, 16- control panel, 17- glass panel, 18- filter opening.
Specific embodiment
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model
Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole
Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are without creative efforts
All other embodiment obtained, fall within the protection scope of the utility model.
Fig. 1-3 is please referred to, the utility model is a kind of device for screening rhizobium, including constant-temperature table 1:
1 output face of constant-temperature table is fixedly connected with just screen box 2；First 2 inner surface of screen box slidably connects waste liquid drawer 3；Just
Filter plate 4 is fixedly connected between 2 inner surface of screen box；Filter plate 4 is located at right above waste liquid drawer 3；4 one surface of filter plate is fixedly connected
There is beaker frame 5；5 inner wall of beaker frame is connected with culture medium 6；
1 two sides of constant-temperature table are fixedly connected to bracket 7；7 one surface of bracket is fixedly connected with secondary screening case 8；Secondary screening case 8
Inner surface offers air hole 9；8 one surface of secondary screening case is fixedly connected there are four weighing sensor 10；Four weighing sensors 10
One surface is fixedly connected to nursery seat 11；Four 11 1 surfaces of nursery seat are fixedly connected to soil moisture humidity sensor
12。
Wherein as Figure 2-3, plant growth lamp 13 is fixedly connected at the top of 8 inner surface of secondary screening case；Secondary screening case 8 is close
One surface of first screen box 2 has been respectively fixedly connected with Temperature Humidity Sensor 14 and ultraviolet disinfection lamp 15.
Wherein, 1 one surface of constant-temperature table is fixedly connected with control panel 16；Compression is fixedly installed inside constant-temperature table 1
Mechanical, electrical machine and eccentric wheel；Constant-temperature table 1 is rotational oscillation formula.
Wherein, adjacent two surface of bracket 7 is fixedly connected to glass panel 17；17 1 surface of glass panel is provided with case
Door.
Wherein, four weighing sensors 10 are in the linear array distribution in 8 one surface of secondary screening case.
Wherein, 4 one surface of filter plate offers filter opening 18；Nine 5 inner walls of beaker frame are fixedly connected to cushion rubber bumper.
One concrete application of the present embodiment are as follows: the Primary Screening Test of drought-enduring rihizobium japonicum is artificial using Macrogol 6000
Artificial drought conditions carry out, and Primary Screening Test carries out in first screen box 12, and four different Macrogol 6000s of test setting are horizontal
Culture medium 6, four corresponding Macrogol 6000 level concentrations of culture medium 6 are respectively 0%, 15%, 25% and 35%；Four
The corresponding flow of water of a culture medium 6 is respectively 0,0.278, -0.699, -1.309MPa, 3 repetitions；Strains tested is picked them separately
1 ring is inoculated into sterilized YMA liquid medium, is placed in constant-temperature table 1, and control panel 17 and Temperature Humidity Sensor are passed through
Thermostat temperature in 14 control constant-temperature tables 1 is 28 DEG C, motor speed is 200rmin oscillation, and inoculation liquid is made in four days in culture,
Adjustment OD value about 0.7 or so, OD is the abbreviation of optical density, indicates the optical density that detected material sponges；Draw 0.1mL inoculation liquid
It accesses in different Macrogol 6000 concentration YMA fluid nutrient mediums, 28 DEG C, the culture of 200rmin constant-temperature table 17 days, then
Sampling is mixed, its OD value is measured at 600mm, its growth and breeding situation is evaluated with the size of OD value；It is needed before measurement with corresponding dense
The YMA culture solution of degree Macrogol 6000 returns to zero to instrument；
Secondary screening test is carried out in nursery seat 11 and secondary screening case 8, by soil potting artificial drought conditions, i.e., according to soil
Earth water content controls coercive intensity, and four nursery seats 11 are handled respectively as four Soil Moisture Gradients: normal moisture: soil contains
Water is the 75%-80% of maxmun field capacity；Moderate water stress: soil moisture content is maxmun field capacity
60%-65%；Moderate water stress: soil moisture content is the 45%-50% of maxmun field capacity；Severe water stress: soil
Earth water content is the 30%-35% of maxmun field capacity.Each gradient sets 3 repetitions, repeats to plant in each nursery seat 11
3 plants of soybean seedling；Rhizobium are prepared into bacteria suspension, cultivates 3-4 days, is then adjusted to unified OD value with liquid YMA medium,
Make cell concentration up to 10 ° of mL.Every basin Rhizobium Inoculation liquid 2mL, not to be inoculated with as control；Daily at dusk using whole weighing method
Soil moisture content is controlled by weighing sensor 10 and soil moisture humidity sensor 12；It is harvested after 40 days, counts root nodule
Quantity measures the dry weight and total nitrogen content of aerial part plant；The model BT-47 of weighing sensor 10；Soil moisture humidity passes
The model RB-02S047A of sensor 12；The model DHT11 of Temperature Humidity Sensor 14.
In the description of this specification, the description of reference term " one embodiment ", " example ", " specific example " etc. means
Particular features, structures, materials, or characteristics described in conjunction with this embodiment or example are contained at least one of the utility model
In embodiment or example.In the present specification, schematic expression of the above terms be not necessarily referring to identical embodiment or
Example.Moreover, particular features, structures, materials, or characteristics described can be in any one or more embodiment or examples
In can be combined in any suitable manner.
The preferred embodiment in the utility model disclosed above is only intended to help to illustrate the utility model.Preferred embodiment is simultaneously
There is no the details that detailed descriptionthe is all, also not limiting the utility model is only the specific embodiment.Obviously, according to this theory
The content of bright book can make many modifications and variations.These embodiments are chosen and specifically described to this specification, is in order to preferably
The principles of the present invention and practical application are explained, so that skilled artisan be enable to better understand and utilize this
Utility model.The utility model is limited only by the claims and their full scope and equivalents.
Claims (6)
1. a kind of device for screening rhizobium, including constant-temperature table (1), it is characterised in that:
Constant-temperature table (1) output face is fixedly connected with just screen box (2)；Described just screen box (2) inner surface slidably connects useless
Liquid drawer (3)；Filter plate (4) are fixedly connected between described just screen box (2) inner surface；The filter plate (4) is located at waste liquid drawer (3)
Surface；(4) one surface of filter plate is fixedly connected with beaker frame (5)；Beaker frame (5) inner wall is connected with culture medium
(6)；
Constant-temperature table (1) two sides are fixedly connected to bracket (7)；(7) one surface of bracket is fixedly connected with secondary screening
Case (8)；Secondary screening case (8) inner surface offers air hole (9)；(8) one surface of secondary screening case is fixedly connected with several titles
It retransmits sensor (10)；Several (10) one surfaces of the weighing sensor are fixedly connected to nursery seat (11)；Several nursery
(11) one surfaces of seat are fixedly connected to soil moisture humidity sensor (12).
2. a kind of device for screening rhizobium according to claim 1, which is characterized in that secondary screening case (8) inner surface
Top be fixedly connected with plant growth lamp (13)；The secondary screening case (8) is respectively fixedly connected with close to a surface of first screen box (2)
There are Temperature Humidity Sensor (14) and ultraviolet disinfection lamp (15).
3. a kind of device for screening rhizobium according to claim 1, which is characterized in that (1) one table of constant-temperature table
Face is fixedly connected with control panel (16)；Compressor, motor and eccentric wheel are fixedly installed inside the constant-temperature table (1)；Institute
Stating constant-temperature table (1) is rotational oscillation formula.
4. a kind of device for screening rhizobium according to claim 1, which is characterized in that adjacent two table of the bracket (7)
Face is fixedly connected to glass panel (17)；(17) one surface of glass panel is provided with chamber door.
5. a kind of device for screening rhizobium according to claim 1, which is characterized in that several weighing sensors
(10) in (8) the one linear array distribution in surface of secondary screening case.
6. a kind of device for screening rhizobium according to claim 1, which is characterized in that open on (4) one surface of filter plate
Equipped with filter opening (18)；Several beaker frame (5) inner walls are fixedly connected to cushion rubber bumper.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821758900.2U CN209065917U (en) | 2018-10-29 | 2018-10-29 | A kind of device screening rhizobium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821758900.2U CN209065917U (en) | 2018-10-29 | 2018-10-29 | A kind of device screening rhizobium |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209065917U true CN209065917U (en) | 2019-07-05 |
Family
ID=67096569
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821758900.2U Active CN209065917U (en) | 2018-10-29 | 2018-10-29 | A kind of device screening rhizobium |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN209065917U (en) |
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2018
- 2018-10-29 CN CN201821758900.2U patent/CN209065917U/en active Active
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