CN105136505A - Method for testing mould removing effect of air purification product - Google Patents
Method for testing mould removing effect of air purification product Download PDFInfo
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- CN105136505A CN105136505A CN201510496450.9A CN201510496450A CN105136505A CN 105136505 A CN105136505 A CN 105136505A CN 201510496450 A CN201510496450 A CN 201510496450A CN 105136505 A CN105136505 A CN 105136505A
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Abstract
The invention discloses a method for testing a mould removing effect of an air purification product. The method comprises the following steps of S1, preparing a mould suspension; S2, adjusting the temperature and the humidity of a testing chamber and a blank chamber, wherein the operation conditions of the testing chamber and the blank chamber are same; S3, equivalently the mold suspension which is prepared in the step S1 into the testing chamber and the blank chamber in a manner of aerosol; S4, respectively collecting initial mould samples in the testing chamber and the blank chamber, starting the air purification product in the testing chamber, keeping the blank chamber in a standing state, respectively residual mould samples in the testing chamber and the blank chamber after a certain time period; and S5, performing culturing and counting for calculating the mould removing effect of the air purification product. The method has advantages of high safety, high reliability, simple operation, low cost and high data stability. The method of the invention can be used for testing the mould removing effect of the air purification product and facilitates air purification product manufacturers, import and export trading and laboratory testing. Furthermore the method fills a technological gap in domestic related testing technology.
Description
Technical field
The invention belongs to analytical test field, particularly relate to the detection method that a kind of air clearing product removes mould effect.
Background technology
Air clearing product is that one effectively can remove indoor air pollutants, and the equipment of the environment that purifies the air of a room, pollutant mainly comprises microorganism, particle and chemical organic vapor.Along with the deterioration of air ambient and people are to the raising of breathing healthy attention rate, rapidly, development presents the trend such as functional diversities, regional characteristics in air clearing product development.
Developing corresponding product purifying property detection technique and method with product technology, there is hysteresis quality in its development.In microbial cleaning Performance Detection, relevant examination criteria and method have " disinfection technology standard " (2002), GB21551.3-2010, Cleaning Principle is by discharging staphylococcus albus in Special test cabin, detect the concentration change before and after purified product purification, and the concentration change detected in standing same time, as blank, calculates the bacterial inactivation rate of air clearing product.
The technique study that mould purifying property is detected is ignored in current micro organism purifying effect detection method.Mould is most eukaryotes, has cell wall structure, and with pre biooxidation ratio, the volume of mycotic spore, growth pattern, eucaryotic cell structure are all not identical with bacterium, and mould has stronger resistance, longer survival period.Mould is adapted at growth and breeding in the environment of southern warm moist, going mouldy of all kinds of article can be caused, change the quality of article, outward appearance, quality or inner structure, the health of serious harm simultaneously, cause the mould allergy of human body and the relevant disease of mycotic infection.
Increasing air clearing product has introduced mould purification function, but through consulting the technique study report and patented claim that all do not find detect mould purifying property.The disappearance of method has hindered development and the popularization of product technology, provide the space of false propaganda also to illegal businessman simultaneously, the very disruptive orderly competition in air clearing product market.
Summary of the invention
In order to fill up the disappearance to mould Assessment of cleaning efficiency method in air clearing product purifying property detection method, the invention provides the detection method that a kind of air clearing product removes mould effect.The method can solve the method problem of air clearing product to mould Assessment of cleaning efficiency, provide the preparation method of mould bacteria suspension, bacteria suspension good stability, be conducive to aerocolloidal generation and diffusion, the biological safety of use warranty test in experimental cabin and blank cabin and the leakproofness of environment, this method introduces blank natural decay rate when calculating purification efficiency, fully ensures objectivity and the authenticity of test result.
The present invention is achieved by the following technical programs.
Air clearing product removes a detection method for mould effect, comprises the steps:
S1. mould bacteria suspension is prepared: prepare fresh mycotic spore suspension, in suspension, add the spreading agent that massfraction is 0.01 ~ 2%;
S2. detect Special test cabin and blank cabin using two identical cabins as purification of air, put into air clearing product to be measured in experimental cabin, regulate the humiture in experimental cabin and blank cabin, experimental cabin is consistent with the service condition in blank cabin;
S3. the mould bacteria suspension prepared by S1 is released in described experimental cabin and blank cabin with aerocolloidal form equivalent, and then fan stirs 5min, leaves standstill 10min;
S4. the initial sample of mould respectively in acquisition test cabin and blank cabin, the air clearing product in starting characteristics test cabin, blank cabin keeps blank state, and the mould after moving to the stipulated time respectively in acquisition test cabin and blank cabin remains sample;
S5. the mould sample that S4 gathers is cultivated, counted, obtain the mould initial concentration in experimental cabin and blank cabin and mould residual concentration respectively, and calculate the mould clean-up effect of described air clearing product.
The present invention is directed to the detection of mould, 0.01 ~ 2% spreading agent is added in the process preparing mycotic spore suspension, inventor finds that the mould bacteria suspension prepared by above-mentioned steps effectively can remove mycelium, form homogeneous, that suspension good, good dispersion degree, spore not easily condense bacteria suspension, be beneficial to the aerocolloidal generation of spore, the mould gasoloid particle diameter of generation be more homogeneous, good stability; The spreading agent added facilitates the homogeneity of gasoloid bacteria containing amount, make two cabins in an initial condition the character of mould and content consistent, the aerocolloidal suspension of mould and stability in cabin can be ensured in test process, provide good basis for detecting air clearing product removal mould effect.
Inventor also optimizes the stirring after release mould gasoloid and time of repose, because stirring and time of repose directly affects the aerocolloidal settleability of mould and aerocolloidal initial concentration, so these two parameters need strict control.Stir and time of repose if extend, aerocolloidal settleability will be increased, reduce aerocolloidal initial concentration, then not reach the requirement of testing conditions.
Preferably, spreading agent described in S1 is one or more in lauryl sodium sulfate, Tween-80 or Tween-20.
Preferably, mould described in S1 is single culture, belongs to the one in aspergillus, Penicillium or interlink spore genus.
Preferably, the rate of ventilation in S2 ~ S4 experimental cabin and blank cabin operational process is less than 0.05h
-1.
Preferably, the mold concentrations scope having left standstill rear experimental cabin and blank cabin described in S3 is 10
4~ 10
6cfu/m
3.
Preferably, the initial specimen sample amount of mould described in S4 is 1 ~ 100L.
Preferably, mould described in S4 remains sample sampling quantity scope is 10 ~ 500L.Preferably, the mould of experimental cabin described in S4 remains sample sampling quantity and is greater than blank cabin.
Preferably, in described S2 ~ S4 process, the temperature in experimental cabin and blank cabin is 20 ~ 30 DEG C, and humidity is 40 ~ 70%RH.
Preferably, the volume in described experimental cabin and blank cabin is 3 ~ 60m
3.Preferably, the volume in described experimental cabin and blank cabin be 3,10,30,60m
3.
Preferably, S5 calculates the computing method of the mould clean-up effect of described air clearing product and is:
Mould purification efficiency=
Wherein, the computing method of natural decay rate are:
Natural decay rate=
Preferably, the humiture in experimental cabin and blank cabin is regulated to be completed by air-conditioning system in cabin described in S2; After in temperature and humidity regulation to specialized range, in subsequent step, the control of humiture is completed by wall heat transfer out of my cabin, and in cabin, air-conditioning system is in closed condition.
Preferably, S2 is by after in temperature and humidity regulation to specialized range, and air-conditioning system in closing chamber, closed in space, opens air-conditioning system out of my cabin, keeps humiture in cabin in specialized range.
Preferably, cultivate described in S5 as sample is cultivated 48h at 25 ~ 30 DEG C.
Compared with prior art, beneficial effect of the present invention is: (1) bacteria suspension suspension is good, the mould gasoloid good stability of generation.(2) biological safety is good, uses purification of air to detect dedicated bay and effectively can prevent the aerocolloidal leakage of mould, while ensureing bio-safety, improve the reliability detecting data.(3) two cabins in experimental cabin and blank cabin operate, and guarantee to test the consistance with blank test condition, testing process is more scientific and reasonable.(4) calculating of purification efficiency, introduce natural decay rate, conclusion is more objective, true.(5) specification reasonable in design of testing process, safe and reliable, simple to operate, with low cost, data stabilization, the mould clean-up effect of air clearing product can be objectively responded, for air clearing product manufacturing enterprise, foreign trade and testing laboratory provide convenience, fill up the technological gap in domestic correlation detection technology field.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in further details, but embodiment does not limit in any form the present invention.Unless stated otherwise, the present invention adopts reagent, method and apparatus are the art conventional reagent, method and apparatus.
embodiment 1
The present embodiment provides a kind of air clearing product to remove the detection method of mould effect, comprises the steps:
S1. prepare mould bacteria suspension: in mycotic culture thing, add normal saline solution, scrape mycotic spore in solution, spore suspension is moved into and is equipped with in the triangular flask of beaded glass, gently after jolting 1min, four layers of filtered through gauze removing mycelia; Spore suspension is through 5000r/min ~ 6000r/min, and centrifugal 20min, removes supernatant, add normal saline solution, then through 5000r/min ~ 6000r/min, centrifugal 20min; Repeat above-mentioned steps, until (400 times) are observed under the microscope, exist without mycelia in suspension, namely prepare fresh mycotic spore suspension, in spore suspension, add 0.01% Tween-80, concussion mixes, and bacterial concentration is 10
7cfu/mL;
S2. Special test cabin and blank cabin is detected using two identical cabins as purification of air, air clearing product to be measured is put in experimental cabin, open the air-conditioning system in experimental cabin and blank cabin, the temperature regulating experimental cabin and blank cabin is respectively 25 DEG C, and humidity is 50%RH simultaneously; Then close the air-conditioning system in two cabins, closed in space, open air-conditioning system out of my cabin, keep the humiture in cabin to control at 20 ~ 30 DEG C respectively, in the scope of 40 ~ 70%RH, experimental cabin is consistent with the service condition in blank cabin, and rate of ventilation is less than 0.05h
-1;
S3. the mould bacteria suspension utilizing aerosol generator to be prepared by S1 is got 1mL respectively and is released in described experimental cabin and blank cabin, then opens fan and stirs 5min, leave standstill 10min;
S4. utilize the initial sample of mould in six grades of sieve aperture impacting type air microorganism samplers difference acquisition test cabins and blank cabin, sampling quantity is 10L; Air clearing product in starting characteristics test cabin, blank cabin keeps blank state, utilize the mould of six grades of sieve aperture impacting type air microorganism samplers respectively in acquisition test cabin and blank cabin to remain sample after moving to the stipulated time, the sampling quantity of experimental cabin is 100L, and the sampling quantity in blank cabin is 20L;
S5. the mould sample that S4 gathers is cultivated 48h, mould plate count in the mold incubator of 26 DEG C, respectively mould initial concentration in experimental cabin and mould residual concentration are respectively 1.57 × 10
5cfu/m
3, 10cfu/m
3; Mould initial concentration in blank cabin and mould residual concentration are respectively 2.62 × 10
5cfu/m
3, 8.43 × 10
4cfu/m
3, according to the changing value of the mold concentrations in blank cabin, calculating natural decay rate is 67.82%.
According to changing value and the natural decay rate of mold concentrations in experimental cabin, the mould purification efficiency calculating air clearing product is 99.98%.
embodiment 2
The present embodiment provides a kind of air clearing product to remove the detection method of mould effect, comprises the steps:
S1. prepare mould bacteria suspension: in mycotic culture thing, add normal saline solution, scrape mycotic spore in solution, spore suspension is moved into and is equipped with in the triangular flask of beaded glass, gently after jolting 1min, four layers of filtered through gauze removing mycelia; Spore suspension is through 5000r/min ~ 6000r/min, and centrifugal 20min, removes supernatant, add normal saline solution, then through 5000r/min ~ 6000r/min, centrifugal 20min; Repeat above-mentioned steps, until (400 times) are observed under the microscope, exist without mycelia in suspension, namely prepare fresh mycotic spore suspension, in spore suspension, add 2% lauryl sodium sulfate, concussion mixes, and bacterial concentration is 10
7cfu/mL;
S2. Special test cabin and blank cabin is detected using two identical cabins as purification of air, air clearing product to be measured is put in experimental cabin, open the air-conditioning system in experimental cabin and blank cabin, the temperature regulating experimental cabin and blank cabin is respectively 20 DEG C, and humidity is 70%RH simultaneously; Then close the air-conditioning system in two cabins, closed in space, open air-conditioning system out of my cabin, keep the humiture in cabin to control at 20 ~ 30 DEG C respectively, in the scope of 40 ~ 70%RH, experimental cabin is consistent with the service condition in blank cabin, and rate of ventilation is less than 0.05h
-1;
S3. the mould bacteria suspension utilizing aerosol generator to be prepared by S1 is got 1mL respectively and is released in described experimental cabin and blank cabin, then opens fan and stirs 5min, leave standstill 10min;
S4. utilize the initial sample of mould in six grades of sieve aperture impacting type air microorganism samplers difference acquisition test cabins and blank cabin, sampling quantity is 50L; Air clearing product in starting characteristics test cabin, blank cabin keeps blank state, utilize the mould of six grades of sieve aperture impacting type air microorganism samplers respectively in acquisition test cabin and blank cabin to remain sample after moving to the stipulated time, the sampling quantity of experimental cabin is 200L, and the sampling quantity in blank cabin is 50L;
S5. the mould sample that S4 gathers is cultivated 48h, mould plate count in the mold incubator of 26 DEG C, respectively mould initial concentration in experimental cabin and mould residual concentration are respectively 1.59 × 10
5cfu/m
3, 20cfu/m
3; Mould initial concentration in blank cabin and mould residual concentration are respectively 2.63 × 10
5cfu/m
3, 8.46 × 10
4cfu/m
3, according to the changing value of the mold concentrations in blank cabin, calculating natural decay rate is 67.83%.
According to changing value and the natural decay rate of mold concentrations in experimental cabin, the mould purification efficiency calculating air clearing product is 99.96%.
comparative example 1
The detection method that a kind of air clearing product that this comparative example provides removes mould effect is basic identical with embodiment 1, and difference is, the spreading agent added in this comparative example S1 is the Tween 80 of 5%.Mould initial concentration in experimental cabin and blank cabin is respectively 2.12 × 10
3cfu/m
3, 1.45 × 10
3cfu/m
3.Mould initial concentration in Laboratory Module after having purified and blank cabin is respectively < 5cfu/m
3, 580cfu/m
3.According to changing value and the natural decay rate of mold concentrations in experimental cabin, calculate the mould purification efficiency of air clearing product for being greater than 98.07%.
Due to, the excessive concentration of spreading agent, the viscosity of bacteria suspension is excessive, is unfavorable for the release of liquid aersol, causes the mould initial concentration in experimental cabin and blank cabin lower, Laboratory Module mould residual concentration after having purified is too low, in the collected specimens of 100L, can't detect mould and remain, therefore, be a scope according to the result that computing formula obtains, instead of the numerical value determined.
comparative example 2
The detection method that a kind of air clearing product that this comparative example provides removes mould effect is basic identical with embodiment 1, and difference is, in this comparative example S3, mixing time is 15min, leaves standstill 25min.Mould initial concentration in experimental cabin and blank cabin is respectively 1.41 × 10
3cfu/m
3, 1.25 × 10
3cfu/m
3.Mould initial concentration in Laboratory Module after having purified and blank cabin is respectively < 5cfu/m
3, 500cfu/m
3.According to changing value and the natural decay rate of mold concentrations in experimental cabin, calculate the mould purification efficiency of air clearing product for being greater than 98.07%.
Due to mixing time and time of repose long, gasoloid has had major part stirring gas shock to cabin body inwall or has been settled down on ground before detection starts, cause the mould initial concentration in experimental cabin and blank cabin lower, Laboratory Module mould residual concentration after having purified is too low, in the collected specimens of 100L, can't detect mould and remain, therefore, be a scope according to the result that computing formula obtains, instead of the numerical value determined.
Claims (9)
1. air clearing product removes a detection method for mould effect, it is characterized in that, comprises the steps:
S1. mould bacteria suspension is prepared: prepare fresh mycotic spore suspension, in suspension, add the spreading agent that massfraction is 0.01%-2%;
S2. detect Special test cabin and blank cabin using two identical cabins as purification of air, put into air clearing product to be measured in experimental cabin, regulate the humiture in experimental cabin and blank cabin, experimental cabin is consistent with the service condition in blank cabin;
S3. the mould bacteria suspension prepared by S1 is released in described experimental cabin and blank cabin with aerocolloidal form equivalent, and then fan stirs 5min, leaves standstill 10min;
S4. the initial sample of mould respectively in acquisition test cabin and blank cabin, the air clearing product in starting characteristics test cabin, blank cabin keeps blank state, and the mould after moving to the stipulated time respectively in acquisition test cabin and blank cabin remains sample;
S5. the mould sample that S4 gathers is cultivated, counted, obtain the mould initial concentration in experimental cabin and blank cabin and mould residual concentration respectively, and calculate the mould clean-up effect of described air clearing product.
2. detection method according to claim 1, is characterized in that, spreading agent described in S1 is one or more in lauryl sodium sulfate, Tween-80 or Tween-20.
3. detection method according to claim 1, is characterized in that, mould described in S1 is single culture, belongs to the one in aspergillus, Penicillium or interlink spore genus.
4. detection method according to claim 1, is characterized in that, the rate of ventilation in described S2 ~ S4 in experimental cabin and blank cabin operational process is less than 0.05h
-1.
5. detection method according to claim 1, is characterized in that, the mold concentrations scope having left standstill rear experimental cabin and blank cabin described in S3 is 10
4~ 10
6cfu/m
3.
6. detection method according to claim 1, is characterized in that, the initial specimen sample amount of mould described in S4 is 1 ~ 100L.
7. detection method according to claim 1, is characterized in that, it is 10 ~ 500L that mould described in S4 remains sample sampling quantity scope.
8. detection method according to claim 1, is characterized in that, in described S2 ~ S4 process, the temperature in experimental cabin and blank cabin is 20 ~ 30 DEG C, and humidity is 40 ~ 70%RH.
9. detection method according to claim 1, is characterized in that, the volume in described experimental cabin and blank cabin is 3 ~ 60m
3.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107764987A (en) * | 2017-09-05 | 2018-03-06 | 广州市微生物研究所 | A kind of air purifier removes the detection method of house dust mite antigen effect |
| CN108094424A (en) * | 2017-12-30 | 2018-06-01 | 赣州市特可环保技术有限公司 | Kill the fumigation plant nanometer fat preparation method and application of air fungus infection virus |
| CN108676779A (en) * | 2018-04-25 | 2018-10-19 | 广州市微生物研究所 | A method of detection air clearing product purifies air pnagus medius ability |
| CN108896337A (en) * | 2018-08-06 | 2018-11-27 | 中国家用电器研究院 | It is a kind of for testing the test macro and test method of new blower degerming performance |
| CN113430247A (en) * | 2021-06-24 | 2021-09-24 | 福建中烟工业有限责任公司 | Method for evaluating mildew-proof effect of tobacco leaves |
| CN114752648A (en) * | 2022-05-25 | 2022-07-15 | 广州市微生物研究所有限公司 | Test method for monitoring propagation microorganisms of indoor humidifying device |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107764987A (en) * | 2017-09-05 | 2018-03-06 | 广州市微生物研究所 | A kind of air purifier removes the detection method of house dust mite antigen effect |
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| CN108676779A (en) * | 2018-04-25 | 2018-10-19 | 广州市微生物研究所 | A method of detection air clearing product purifies air pnagus medius ability |
| CN108896337A (en) * | 2018-08-06 | 2018-11-27 | 中国家用电器研究院 | It is a kind of for testing the test macro and test method of new blower degerming performance |
| CN113430247A (en) * | 2021-06-24 | 2021-09-24 | 福建中烟工业有限责任公司 | Method for evaluating mildew-proof effect of tobacco leaves |
| CN114752648A (en) * | 2022-05-25 | 2022-07-15 | 广州市微生物研究所有限公司 | Test method for monitoring propagation microorganisms of indoor humidifying device |
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Application publication date: 20151209 |
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