CN202072704U - Air sterilization effect evaluation system - Google Patents

Abstract

The air disinfection effect evaluation system involves a closed system that simulates the generation and sampling of microbial aerosols. In order to solve the biological safety problem in the microbial aerosol experiment, and evaluate the air disinfection effect more effectively, the air disinfection effect evaluation system is characterized in that: the microbial aerosol generating equipment is connected to the airtight air-tight air filter equipped with the air filter through the pipeline. The sampling equipment forms a loop through the pipeline and the airtight cabinet. The entire system is biologically isolated from the surrounding environment. Moreover, the residual microbial aerosol after passing through the sampler will not be discharged to the external environment, but will flow back into the airtight cabinet. After the experiment, it can be disinfected in the cabinet. The system can be installed in the BSL-1 laboratory, and it can also be used in general laboratories if non-pathogenic microorganisms, especially defective non-pathogenic microorganisms, are used. In addition, the application of the utility model can produce a stable aerosol natural attenuation curve, so as to standardize and compare disinfection experiments.

Description

Air disinfection effect evaluation system

technical field

The utility model relates to a closed system for simulating the generation and sampling of microbial aerosols, which is used for evaluating the killing effect of air purification products on microorganisms in the air.

Background technique

In recent years, respiratory infectious diseases transmitted through the air have posed a serious threat to human health, and people have paid more and more attention to the air quality of their environment. Therefore, various air disinfection products have emerged as the times require, and there are other products for the purpose of air disinfection. New materials and new products are also being researched and developed. Therefore, an effective air disinfection effect evaluation method is extremely necessary for the research and development of new materials and products, and for the supervision and inspection of product quality.

The basic point of the air disinfection effect test is to compare the changes in the number of living microorganisms contained in a certain amount of aerosol samples before and after disinfection. For the detection of microorganisms in aerosols, due to the instability of aerosols, relatively difficult sampling and difficult protection, there is still a lack of a unified and convenient means and equipment for implementation. The traditional air microbial sampling method is mainly the natural sedimentation method. In recent years, there have been room tests using biological aerosol samplers after the artificial generation of microbial aerosols, which has improved the evaluation efficiency of air disinfection tests compared with the traditional natural sedimentation method. However, the room test is limited by the building structure such as the ventilation system, and the entry and exit of personnel cannot be avoided during the test process. The leakage of microbial aerosols is impossible to avoid, which is dangerous to the surrounding environment and personnel.

Utility model content

In order to solve the biological safety problem in the microbial aerosol experiment and evaluate the air disinfection effect more effectively, we established a new air disinfection effect evaluation system. The system organically integrates microbial aerosol generating equipment, air-tight cabinets for air disinfection products, and sampling equipment, and the entire system is biologically isolated from the surrounding environment. Moreover, the residual microbial aerosol after passing through the sampler will not be discharged to the external environment, but will flow back into the airtight cabinet. After the experiment, it can be disinfected in the cabinet. The system can be installed in the BSL-1 laboratory, and it can also be used in general laboratories if non-pathogenic microorganisms, especially defective non-pathogenic microorganisms, are used. In addition, it is difficult to control the error caused by the natural attenuation of the aerosol in the current air disinfection experiment using the artificial microbial aerosol. The controllability of the particle size and microbial concentration in the aerosol produced by the utility model is enhanced, and the application of the new model can make stable Aerosol natural attenuation curves for standardized comparison of disinfection experiments.

The air disinfection effect evaluation system provided by the utility model is characterized in that the microbial aerosol generating equipment is connected to an airtight cabinet provided with an air filter through a pipeline, and the sampling equipment forms a loop through the pipeline and the airtight cabinet.

According to GB13554-92 "High Efficiency Air Filter", the air filter installed on the airtight cabinet can block particles with a particle size of ≥0.1 μm inside and outside the cabinet (efficiency ≥99.999%), which includes viruses and bacteria, so it will not cause the leakage of microorganisms .

The technical scheme that the utility model solves its technical problem adopts is:

1. Organically integrate microbial aerosol generating equipment, air-tight cabinets for air disinfection products, and sampling equipment, and the entire system is biologically isolated from the surrounding environment;

2. Place the sampling pump of the microbial aerosol sampler in the airtight cabinet, so that the residual aerosol after sampling flows back into the airtight cabinet, and the sampling head is placed outside the cabinet to facilitate the sampling operation;

3. The natural attenuation curves of different kinds of microbial aerosol particles can be prepared respectively, and standardized comparison can be carried out.

Description of drawings

Figure 1 The overall schematic diagram of the air disinfection effect evaluation system

Among them, 1-microbial aerosol generating equipment; 2-airtight cabinet, 3-air filter, 4-sampling equipment.

Detailed ways

Figure 1 shows the integration of microbial aerosol generating equipment, air-tight cabinets for air disinfection products, and sampling equipment in the air disinfection effect evaluation system. The arrows indicate the flow direction of the aerosol.

1 After the system is installed, the air tightness test must be carried out first. After atomizing high-concentration specific microbial aerosols in the airtight cabinet, set at least 4 sampling points at 1m around the cabinet to collect air microbial samples.

2 Determine the conditions under which the bioaerosol generator produces inhalable aerosol particles.

3 According to the purpose of disinfection evaluation, select suitable varieties of non-pathogenic microorganisms (bacteria, fungi or phage) for cultivation. Take 1-10 μl of culture solution and dilute it to a suitable concentration of microbial aerosol generating solution (10ml), place the atomizing cup in the airtight cabinet, and close the airtight cabinet. Turn on the control button of the aerosol generator outside the cabinet and turn it off after 5 minutes.

4 Start the sampler outside the cabinet from the moment of atomization, and collect microbial aerosol samples in the cabinet at regular intervals (such as 30 minutes), each time lasting 30 seconds or 1 minute. According to the culture results after sampling, the natural attenuation curve of microbial aerosol particles within 1 h after atomization was made.

5 Pre-place the air disinfection product to be evaluated in the airtight cabinet. Microbial aerosol occurs, same as 3. Turn on the air sterilizer and sample at the same time, same as 4.

6 According to the culture results after sampling, the attenuation curve of microbial aerosol particles within 1 hour after atomization is made when the air sterilizer is turned on. Figure 2 shows that after the sterilizer has been used for a certain period of time, the number of microorganisms in the aerosol can be compared with the natural attenuation conditions for an intuitive comparison. It is also possible to calculate and compare the list of sampling results under the action of the natural attenuation and the air sterilizer to calculate the kill rate of the sterilizer for microorganisms in a certain action time, and the results obtained in this way correct the influence of the natural attenuation of microbial aerosols on the experimental results , as shown in Table 1, after deducting the influence of natural attenuation factors, the killing rates of the air purifier on the bacteria in the aerosol in the cabinet after 30, 60 and 90 minutes of operation were 81.50%, 99.48% and 100%, respectively.

Claims (1)

1. air sterilization effect evaluation system, it is characterized in that: microbial aerosol generation equipment is connected to the airtight cabinet that is provided with air filter by pipeline, and sample devices forms the loop by pipeline and airtight cabinet.

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