CN108094424A - Kill the fumigation plant nanometer fat preparation method and application of air fungus infection virus - Google Patents
Kill the fumigation plant nanometer fat preparation method and application of air fungus infection virus Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/18—Vapour or smoke emitting compositions with delayed or sustained release
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/06—Oxygen or sulfur directly attached to a cycloaliphatic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aliphatically bound aldehyde or keto groups, or thio analogues thereof; Derivatives thereof, e.g. acetals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/06—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing keto or thioketo groups as part of a ring, e.g. cyclohexanone, quinone; Derivatives thereof, e.g. ketals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/16—Heavy metals; Compounds thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
Abstract
Kill the stifling plant nanometer fat preparation method and application of air fungus infection virus, the fumigation plant nanometer fat that raw material is configured to by Cortex Eucommiae fat, hydroxyl cyclopentadecanone, senlciphylline, high-purity L-borneol, nano silver colloidal sol, Moschus, cinnamic acid.Solves mould, Legionella, infectiousness germ, the originality of virus in all-pervasive stifling killing room air.Solves the originality for being killed in crowd using constant dynamic high-efficiency and infecting influenza, killing person to person's respiratory infectious.Realize man-machine same room, product is nontoxic, without side-effects, stifling plant nanometer fat, 1 30 nanoparticle suspension of sublimation gases reaches in air indoors kills harmful levels of pathogens, virus, the propagation of prevention disease, applied to uses such as air-conditioning, automobile, air purifier, high ferro, train, aircraft, steamer, container, room, school, You Eryuan, hospital, hotel, public places, have and have a extensive future.
Description
Technical field
The present invention relates to a kind of constant all-pervasive efficient killing air mould virosis bacterium plant of energy in crowd is new
The fumigation plant nanometer fat that material, especially plant extract sterilization the PO factors are formulated, it is net applied to air-conditioning, automobile, air
What change device, high ferro, train, aircraft, steamer, container, room, school, You Eryuan, hospital, hotel, public place etc. used kills
The fumigation plant nanometer fat preparation method and application for air fungus infection virus of going out.
Background technology
Domestic and international sterilised products ignore 18 kinds of mycotoxins and are detrimental to health in air.Mould is eucaryote
Body has cell wall structure, and compared with bacterium, volume, growth pattern, eucaryotic cell structure and the bacterium of mycotic spore differ,
Mould has stronger resistance, longer survival period.Mould is suitble to growth and breeding in the environment of warm moist, can cause each
Class article goes mouldy, and changes quality, appearance, quality or the internal structure of article, it is important to note that cannot killing of having of sterilised products
It goes out mould, while ignores mycotoxin and seriously endanger health, the mould allergy for causing human body is related to mycotic infection
Disease.
At present, domestic and international sterilised products species is more, most of to kill mould, nor extracted form natural plant sterilization because
Son is configured to sterilization material;Some sterilised products bactericidal effects are good but toxicity is also big, cannot be used in crowd, during the crowd is dense
It can not accomplish that continuation is sterilized；Some crystal volatilization bactericidal effects are good, have application on human skin knot to touch, and can stimulate skin allergy, cost
Height, complex production process, investment goods are more；Some need fever tablet heating fumigating is sterilized, and use is dangerous, without fumigating naturally
Volatilization function is unable to continuation sterilization, inconvenient for use, costly；The air such as ozone, chlorine, chlorosulfuric acid, bromomethane, formaldehyde kill
Also result in great injury while bacterium to human body, people must be from scene during sterilization, it is impossible to which continuation is sterilized in crowd, also
Irritant, corrosivity causes secondary pollution to environment；Some smog sterilization very trouble is unable to continuation sterilization, during sterilization
People must come out scene；Some toxicity is relatively small, but does not have the technical disadvantages such as bactericidal effect or effect unobvious at all.
In recent years, all-pervasive stifling plant nanometer fat air fumigate PO factor materials research become air killing it is mould
Bacterium, the new hot spot for killing infection through air venereal bacteria virus.The present invention fumigated killing fungus sterilization by the research plant factor of 13 years
It kills the virus, by thousands of experiments, change takes millions of members, and finally unexpected efficiently fumigating kills air mould, kills army
Group bacterium, killing infectiousness germ virus are succeeded in developing, and researcher has consulted domestic and international paper and patent, to there is presently no certainly
So stifling property plant nanometer fat, it is even to this day both at home and abroad or empty for killing the innovation and creation of air mould virus harmful bacteria
In vain.
The content of the invention
It is an object of the invention to：In view of the above problems, air mould can be killed and kill by providing one kind
The fumigation plant nanometer fat that the plant extract sterilization factors of air germ virus are formulated, in crowd using, can accomplish it is non-porous
Not entering to fumigate and kill mould, Legionella, infectiousness germ, virus in air, persistence leaves no residue object, has a wide range of application, and
And the fumigation plant nanometer fat preparation side of the killing air fungus infection virus of nontoxic, without side-effects, constant dynamic high-efficiency
Method and application.Kill the fumigation plant nanometer fat preparation method and application of air fungus infection virus, which is characterized in that by following
The material combination of weight proportion is made：It is 7-16 grams of Cortex Eucommiae fat, 1-6 grams of hydroxyl cyclopentadecanone, 2-11 grams of senlciphylline, high-purity
Spend 6-12 grams of L-Borneol, 0.1-6 grams of nano silver colloidal sol, 0.001-0.03 grams of Moschus, 1-11 grams of cinnamic acid.
It prepares the optimal of plant nanometer fat for killing mould virosis bacterium and selects raw material weight number meter ratio range, feature
It is, 10 grams of Cortex Eucommiae fat, 3 grams of senlciphylline, 8 grams of high-purity L-borneol, 3 grams of hydroxyl cyclopentadecanone, nano silver colloidal sol 2
Gram, 0.002 gram of Moschus, 8 grams of cinnamic acid.
The fumigation plant nanometer fat preparation method and application of the present invention for killing air fungus infection virus, first prepares
Raw material is as follows：
1st, Cortex Eucommiae fat preparation method:
1) seed of bark of eucommia 100g after drying is weighed, is crushed to 80 mesh, is then 9 according to the weight ratio of petroleum ether and seed of bark of eucommia:
1.5 ratio, dried seed of bark of eucommia is fitted into extraction reaction kettle, with liquid nitrogen controlled at -11 DEG C, adds in petroleum ether
1100ml is extracted, and extract is the semi-finished product eucommia ulmoides seed oil of sepia, and extract is conventional through greases such as decoloration, debrominates
Treatment process obtains faint yellow finished product eucommia ulmoides seed oil;
2) dregs of fat obtained after step 1) is extracted are dried, and the dried dregs of fat are put into extraction reaction kettle and add in ethanol water
Solution concentration is 85%, 1100ml, is extracted, and extract obtains after drying, eucommia ulmoides extract;
3) the cream drying that will be obtained after step 2) extraction, dried cream is put into extraction reaction kettle and to add in ethyl acetate molten
Agent 1000ml, 80 DEG C are extracted, and extract is brownish black thick liquid, after extract drying, obtains Cortex Eucommiae fat.
2nd, senlciphylline preparation method;
Climbing groundsel medicinal raw material 10kg is taken, is crushed to 60 mesh, adds in 85% ethanol solution refluxing extraction 5 times, merges extracting solution, adds
Enter the aluminium oxide color of the 2% of its volume, be obtained by filtration destainer, destainer is concentrated into medicinal extract, medicinal extract with 3% citric acid water
Solution dissolves, filtering, and HD-8 cation exchange resins on filtrate are first washed with deionized water to neutrality, then with 60L, pH11's
70% ethanol solution elutes, and eluent concentration adjusts pH9.5, lets cool crystallization, crystallization, crystallization methylene chloride reflux is obtained by filtration
Dissolving, filters, lets cool crystallization, filter out crystallizing and drying up to senlciphylline while hot;
3rd, high-purity L-borneol preparation method;
Step 1, natural crude product L-Borneol, L-Borneol content 63-67% are dried after centrifuge dripping；
Above-mentioned solid is dissolved in 2 times of 95% ethyl alcohol of amount by step 2；
Step 3, cool down and 0.03 times of amount (weight of crude product L-Borneol) metallic sodium is added portionwise into above-mentioned solution, after finishing
When 0 DEG C of reaction 3 is small；
Step 4 is poured into above-mentioned reaction solution in 5 times of amount (weight of crude product L-Borneol) water, when standing 1.5 is small, filters；
Step 5, gained filter cake is added in 2 times of amount (weight of crude product L-Borneol) petroleum ethers, is heated to 60 DEG C, is divided sub-cloud
Water phase, organic phase are cooled to -10 DEG C, a large amount of flat crystals occur.Filtering, dries, obtains high-purity L-borneol, content is
99.97%.
4th, hydroxyl cyclopentadecanone preparation method;
The first of 40ml dryings is added in into the four-neck flask of the 250ml dryings with stirring, thermometer, condenser pipe, dropping funel
Benzene, 1.60g is added in into flask, and 0.07mol metallic sodiums under nitrogen protection, are to slowly warm up to toluene boiling, quickly stir 5
Minute, sodium is broken into silvery white fine particles.Temperature is controlled at 90 DEG C, is added dropwise dissolved with 4.38g (0.01mol) tetrapropyl 15
The 40ml toluene solutions of carbon diamides, after 4 are added dropwise when small, when insulation reaction 3 is small.After reaction, the anhydrous second of 8mL is added dropwise
Alcohol decomposes remaining metallic sodium.Insoluble matter is filtered to remove, filtrate water is washed till neutrality, and vacuum distillation removes solvent, obtains hydroxyl ring
15 ketone.
5th, nano silver colloidal sol preparation method;
0.5g Chinese yam starch is taken to be scattered in 10ml deionized waters, is stirred at 80 DEG C to starch solution is gelatinized to obtain, added it to
20ml concentration is in the silver ammino solution of 1wt%, is uniformly mixed and obtains starch-silver ammonia composite solution, composite solution is placed in-
Freezing processing under the conditions of 20 DEG C makees pulverization process after its icing, obtains ice powder.
100 grams of crushing of aloe, filtering take liquid, are scattered in deionized water after removing the peel, and it is 10 wt to prepare 300ml concentration
The extract solution from aloe aqueous solution of %, potassium hydroxide solution adjust its pH to 12 and obtain alkaline extract solution from aloe；Above-mentioned ice powder is added
It is added in alkaline extract solution from aloe, 50 DEG C of stirring 400min obtain nano silver colloidal sol；
The fumigation plant nanometer fat preparation method and application of the present invention for killing air fungus infection virus, fumigates plant nanometer
The preparation method of fat, spy be, 10 grams of Cortex Eucommiae fat, 3 grams of senlciphylline, 8 grams of high-purity L-borneol, hydroxyl ring 15
3 grams of ketone, 2 grams of sodium rice silver sol, 0.005 gram of Moschus are incorporated in integral crushing, and 8 grams of heating of cinnamic acid are added in crushed material and are melted,
It is thoroughly mixed after melting, lysate flows into plastic casing, stifling plant nanometer fat is obtained, applied to air-conditioning, automobile, air purification
The uses such as device, high ferro, train, aircraft, steamer, container, room, school, You Eryuan, hospital, hotel, public place.
1st, stifling plant nanometer fat of the invention is to animal toxicity test;
For stifling plant nanometer fat through toxicology neasuring, small white mouse LD50 of passing through mouth is 5123.3 mgs/kg, and hMLS experiments belong to sun
Property, long term toxicity test proves no carcinogenic, teratogenesis, phenomena such as mutagenesis, thus it is nontoxic to people and animals.
2nd, plant nanometer fat acute toxicity test is fumigated:
Using the stifling plant nanometer fat of the present invention as object, the small white mouse of the identical health of 20 vital signs is taken to be tested, by 20
Small white mouse is divided into four groups, and one group of control group and three groups of test groups carry out feeding, by medicine to 15 small white mouses of test group respectively
In the feed of object incorporation small white mouse, 20 small white mouses are controlled with same life conditions, first group of 1 times for clinical day usual amounts
Dosage, second group be 2 multiple doses, three groups be 3 multiple doses, then observe each group, by 24 when small after observation, 10 little Bai
Mouse is still healthy, and activity, diet, hair, excreta are no abnormal, have no toxic reaction.
3rd, plant nanometer fat long term toxicity test is fumigated;
Using the stifling plant nanometer fat of the present invention as object, adult rat 50 is selected, is randomly divided into 5 groups, one group of control group and four
Group test group, every group 10, half male and half female.Test group each group dosage is respectively the 0.1 of LD50（500m/K parts of BW）、0.15
（750m/K parts of BW）、0.22（1100m/K parts of BW）、0.34（1700m/K parts of BW）, daily gavage once, continuous 20
My god.Then observe 7 days.Without death, each dosage group does not show dead without dose-response relationship, sheet testing result each group yet
Drug does not have obvious accumulative toxicity, and long-term use will not generate side effect.
4th, plant nanometer fat hypersensitive test is fumigated;
Using the stifling plant nanometer fat of the present invention as object, 30 healthy guinea pigs is taken to be tested, male and female half-and-half is gone before administration
The processing of back both sides unhairings, is randomly divided into five groups, every group 6, is respectively control group, the high 1 multiple dose 0.5mg of damaged skin, low
2 multiple dose 1mg groups of dosage, the high 1 multiple dose 0.5mg of intact skin, 2 multiple dose 1mg groups of low dosage.Respectively to four groups of progress below
After subcutaneous administration, once a day, continuous 7 days, each group situation is observed, as a result cavy all survives, and the skin of the cavy of each group
Skin does not occur any discomfort, and apparent exception does not occur for the searching for food of cavy, activity, breathing and weight etc..The results show that the medicine
Object does not have sensitization.Pharmacology pharmacodynamic experiment display, the medicine do not have any toxic action, sensitization and adverse reaction, face
Bed is safe to use.
5th, fumigate plant nanometer fat and kill staphylococcus albus examining report：
Staphylococcus albus 99.82% is killed as can be seen from the table.
6th, fumigate plant nanometer fat and kill staphylococcus aureus examining report：
Staphylococcus aureus 99.99% is killed as can be seen from the table.
7th, stifling plant nanometer fat is put into sky gas-purifying device killing mould experiment effect and experimental method, step are as follows：
1st, mould bacteria suspension is prepared：Normal saline solution is added in into mycotic culture object, scrapes mycotic spore in solution, by spore
Sub- suspension is moved into the triangular flask equipped with bead, and after gently shaking 1min, four layers of filtered through gauze remove mycelia；Spore suspension
Through 5000 r/min~6000 r/min, 20min is centrifuged, removes supernatant, adds in normal saline solution, then through 5000 r/
The r/min of min~6000 centrifuges 20min；It repeats the above steps, until under the microscope（400 times）It observes, it is sterile in suspension
Silk exists, that is, fresh mycotic spore suspension is prepared, and 0.01% Tween-80 is added in spore suspension, and concussion mixing is equal
It is even, bacterial concentration 107cfu/mL；
2nd, Special test cabin and blank cabin are detected using two identical cabins as air purification, is put into experimental cabin to be measured smoked
Plant nanometer fat air purifier is steamed, while opens the air-conditioning system in experimental cabin and blank cabin, adjusts experimental cabin and sky respectively
The temperature in white cabin is 25 DEG C, humidity 50%RH；Air-conditioning system out of my cabin is opened by the air-conditioning system being then shut off in two cabins, closed in space
System keeps the humiture in cabin to be controlled respectively at 20 ~ 30 DEG C, in the range of 40 ~ 70%RH, the operation item in experimental cabin and blank cabin
Part is consistent, and rate of ventilation is less than 0.05h-1；
3rd, 1mL is taken to discharge to the experimental cabin and blank respectively the mould bacteria suspension that 1 is prepared using aerosol generator
In cabin, fan stirring 5min is then opened, stands 10min；
4th, the initial sample of mould in experimental cabin and blank cabin is gathered respectively using six grades of sieve pore impacting type air microorganism samplers
This, sampling quantity 10L；Stifling plant nanometer fat air purifier in firing test cabin, blank cabin keep blank state, operation
After to the stipulated time six grades of sieve pore impacting type air microorganism samplers is utilized to gather the mould in experimental cabin and blank cabin respectively
Sample is remained, the sampling quantity of experimental cabin is 100L, and the sampling quantity in blank cabin is 20L；
5th, the mould sample by 4 acquisitions cultivates 48h, mould plate count in 26 DEG C of mold incubator, obtains experimental cabin respectively
In mould initial concentration and mould residual concentration be respectively 1.57 × 105cfu/m3,10 cfu/m3；Mould in blank cabin
Initial concentration and mould residual concentration are respectively 2.62 × 105cfu/m3,8.43 × 104cfu/m3, and stifling plant nanometer fat is put
It is 99.97% to enter air purifier to kill mould efficiency.
Killing fungus mechanism:
It is formed in stifling plant nanometer fat there are three types of molecule and kills mould;(1) calcium in vegetable butter, discharges propanoic acid molecules first,
The latter can form hypertonic environment outside fungal cell is dehydrated mould, and loses fertility, can also enter fungal cell, suppression
The activity of desmoenzyme processed, so as to press down mycostatic breeding.(2) the sour sodium in plant nanometer fat is by formic acid bioactive molecule
Fungal cell is pierced into, inhibit respiratory enzyme and hinders tricarboxylic acid cycle process, while the also permeability of block cell film, and is reached
Inhibit the effect of mould breeding.(3) ester in plant nanometer fat can penetrate fungal cell film, inhibit cell division, inhibit sugar
The activity of key enzyme in metabolism reaches mould proof killing mould effect so as to press down mycostatic respiration, mould with efficiently removing
Function of odor, and except musty effect is lasting.
Sterilization mechanism:
Stifling plant nanometer fat, containing plant factor, catalytic capability generates active oxygen around bacterium, can sterilize, at the same sterilize because
Son can be attached on bacterial cell membrane, rapidly to intracellular penetration, hindered bacterial growth synthesis, included various sterilization factors and activity
The factor has the strong effect for killing germ virus.
The advantageous effect of the stifling plant nanometer fat of the present invention：
1st, the crucial natural fumigation lawn plant nanometer fat for solving the factor synthesis of extraction plant sterilization, efficiently kills white grapes
Coccus, staphylococcus aureus, Legionella, kerekou pneumonia primary, typhoid fever virus, Escherichia coli, disease carrying germ virus, harmful bacteria
Killing rate is created up to the novel of 99.82-99.99%.
2nd, breach stifling plant nanometer fat and be put into air purifier, air-conditioning filter net etc., have except musty kills air
Middle mould efficiency is 99.97% new creation.
3rd, it is crucial solve it is all-pervasive it is stifling kill mould in room air, Legionella, infectiousness germ, virus it is only
It is invasive.
4th, it is crucial solve in crowd to kill using constant dynamic high-efficiency infect influenza, person to person's respiratory infectious
Originality.
5th, it is found that man-machine same room, the sublimation gases that product is nontoxic, without side-effects, stifling plant nanometer fat is unexpected
1-30 nanoparticle suspensions reach in air indoors to be killed mould, kills harmful levels of pathogens, the propagation of prevention disease and virus and intersect sense
Dye, reduces the new creation of Expectancy.
6th, the new invention is compared with domestic and international other indoor sterilization products, have prominent substantive distinguishing features and significantly into
Step, can generate good effect and benefit, have and have a extensive future.
Specific embodiment：
The invention will be further described for example below.
Embodiment 1：
Kill the stifling plant nanometer fat preparation method and application of air fungus infection virus, which is characterized in that by following weight
The material combination of proportioning is made：7-16 grams of Cortex Eucommiae fat, 1-6 grams of hydroxyl cyclopentadecanone, 2-11 grams of senlciphylline, high-purity are left
Revolve 6-12 grams of borneol, 0.1-6 grams of nano silver colloidal sol, 0.001-0.03 grams of Moschus, 1-11 grams of cinnamic acid.
It prepares the optimal of plant nanometer fat for killing mould virosis bacterium and selects raw material weight number meter ratio range, feature
It is, 10 grams of Cortex Eucommiae fat, 3 grams of senlciphylline, 8 grams of high-purity L-borneol, 3 grams of hydroxyl cyclopentadecanone, nano silver colloidal sol 2
Gram, 0.002 gram of Moschus, 8 grams of cinnamic acid.
The fumigation plant nanometer fat preparation method and application of the present invention for killing air fungus infection virus, first prepares
Raw material is as follows：
1st, Cortex Eucommiae fat preparation method:
1) seed of bark of eucommia 100g after drying is weighed, is crushed to 80 mesh, is then 9 according to the weight ratio of petroleum ether and seed of bark of eucommia:
1.5 ratio, dried seed of bark of eucommia is fitted into extraction reaction kettle, with liquid nitrogen controlled at -11 DEG C, adds in petroleum ether
1100ml is extracted, and extract is the semi-finished product eucommia ulmoides seed oil of sepia, and extract is conventional through greases such as decoloration, debrominates
Treatment process obtains faint yellow finished product eucommia ulmoides seed oil;
2) dregs of fat obtained after step 1) is extracted are dried, and the dried dregs of fat are put into extraction reaction kettle and add in ethanol water
Solution concentration is 85%, 1100ml, is extracted, and extract obtains after drying, eucommia ulmoides extract;
3) the cream drying that will be obtained after step 2) extraction, dried cream is put into extraction reaction kettle and to add in ethyl acetate molten
Agent 1000ml, 80 DEG C are extracted, and extract is brownish black thick liquid, after extract drying, obtains Cortex Eucommiae fat.
2nd, senlciphylline preparation method;
Climbing groundsel medicinal raw material 10kg is taken, is crushed to 60 mesh, adds in 85% ethanol solution refluxing extraction 5 times, merges extracting solution, adds
Enter the aluminium oxide color of the 2% of its volume, be obtained by filtration destainer, destainer is concentrated into medicinal extract, medicinal extract with 3% citric acid water
Solution dissolves, filtering, and HD-8 cation exchange resins on filtrate are first washed with deionized water to neutrality, then with 60L, pH11's
70% ethanol solution elutes, and eluent concentration adjusts pH9.5, lets cool crystallization, crystallization, crystallization methylene chloride reflux is obtained by filtration
Dissolving, filters, lets cool crystallization, filter out crystallizing and drying up to senlciphylline while hot;
3rd, high-purity L-borneol preparation method;
Step 1, natural crude product L-Borneol, L-Borneol content 63-67% are dried after centrifuge dripping；
Above-mentioned solid is dissolved in 2 times of 95% ethyl alcohol of amount by step 2；
Step 3, cool down and 0.03 times of amount (weight of crude product L-Borneol) metallic sodium is added portionwise into above-mentioned solution, after finishing
When 0 DEG C of reaction 3 is small；
Step 4 is poured into above-mentioned reaction solution in 5 times of amount (weight of crude product L-Borneol) water, when standing 1.5 is small, filters；
Step 5, gained filter cake is added in 2 times of amount (weight of crude product L-Borneol) petroleum ethers, is heated to 60 DEG C, is divided sub-cloud
Water phase, organic phase are cooled to -10 DEG C, a large amount of flat crystals occur.Filtering, dries, obtains high-purity L-borneol, content is
99.97%.
4th, hydroxyl cyclopentadecanone preparation method;
The first of 40ml dryings is added in into the four-neck flask of the 250ml dryings with stirring, thermometer, condenser pipe, dropping funel
Benzene, 1.60g is added in into flask, and 0.07mol metallic sodiums under nitrogen protection, are to slowly warm up to toluene boiling, quickly stir 5
Minute, sodium is broken into silvery white fine particles.Temperature is controlled at 90 DEG C, is added dropwise dissolved with 4.38g (0.01mol) tetrapropyl 15
The 40ml toluene solutions of carbon diamides, after 4 are added dropwise when small, when insulation reaction 3 is small.After reaction, the anhydrous second of 8mL is added dropwise
Alcohol decomposes remaining metallic sodium.Insoluble matter is filtered to remove, filtrate water is washed till neutrality, and vacuum distillation removes solvent, obtains hydroxyl ring
15 ketone.
5th, nano silver silver sol preparation method;
0.5g Chinese yam starch is taken to be scattered in 10ml deionized waters, is stirred at 80 DEG C to starch solution is gelatinized to obtain, added it to
20ml concentration is in the silver ammino solution of 1wt%, is uniformly mixed and obtains starch-silver ammonia composite solution, composite solution is placed in-
Freezing processing under the conditions of 20 DEG C makees pulverization process after its icing, obtains ice powder.
100 grams of crushing of aloe, filtering take liquid, are scattered in deionized water after removing the peel, and it is 10 wt to prepare 300ml concentration
The extract solution from aloe aqueous solution of %, potassium hydroxide solution adjust its pH to 12 and obtain alkaline extract solution from aloe；Above-mentioned ice powder is added
It is added in alkaline extract solution from aloe, 50 DEG C of stirring 400min obtain nano silver colloidal sol；
The fumigation plant nanometer fat preparation method and application of the present invention for killing air fungus infection virus, fumigates plant nanometer
The preparation method of fat, spy be, 10 grams of Cortex Eucommiae fat, 3 grams of senlciphylline, 8 grams of high-purity L-borneol, hydroxyl ring 15
3 grams of ketone, 2 grams of nano silver colloidal sol, 0.005 gram of Moschus are incorporated in integral crushing, and 8 grams of heating of cinnamic acid are added in crushed material and are melted,
It is thoroughly mixed after melting, lysate flows into plastic casing, stifling plant nanometer fat is obtained, applied to air-conditioning, automobile, air purification
The uses such as device, high ferro, train, aircraft, steamer, container, room, school, You Eryuan, hospital, hotel, public place.
Claims (4)
1. a kind of stifling plant nanometer fat preparation method for killing air fungus infection virus, which is characterized in that by following weight
The material combination of proportioning is made：7-16 grams of Cortex Eucommiae fat, 1-6 grams of hydroxyl cyclopentadecanone, 2-11 grams of senlciphylline, high-purity are left
Revolve 6-12 grams of borneol, 0.1-6 grams of nano silver colloidal sol, 0.001-0.03 grams of Moschus, 1-11 grams of cinnamic acid;
2. raw material weight number meter matches according to claim 1, which is characterized in that 10 grams of Cortex Eucommiae fat, senlciphylline
3 grams, 8 grams of high-purity L-borneol, 3 grams of hydroxyl cyclopentadecanone, 2 grams of nano silver colloidal sol, 0.002 gram of Moschus, 8 grams of cinnamic acid;
3. killing the stifling plant nanometer fat preparation method of air fungus infection virus according to claim 1, feature exists
In first preparing raw material：
(1) Cortex Eucommiae fat preparation method:
1st, seed of bark of eucommia 100g after drying is weighed, is crushed to 80 mesh, is then 9 according to the weight ratio of petroleum ether and seed of bark of eucommia:
1.5 ratio, dried seed of bark of eucommia is fitted into extraction reaction kettle, with liquid nitrogen controlled at -11 DEG C, adds in petroleum ether
1100ml is extracted, and extract is the semi-finished product eucommia ulmoides seed oil of sepia, and extract is conventional through greases such as decoloration, debrominates
Treatment process obtains faint yellow finished product eucommia ulmoides seed oil;
2nd, the dregs of fat obtained after step 1) is extracted are dried, and the dried dregs of fat are put into extraction reaction kettle and add in ethanol water
Solution concentration is 85%, 1100ml, is extracted, and extract obtains after drying, eucommia ulmoides extract;
3rd, the cream drying that will be obtained after step 2) extraction, dried cream is put into extraction reaction kettle and to add in ethyl acetate molten
Agent 1000ml, 80 DEG C are extracted, and extract is brownish black thick liquid, after extract drying, obtains Cortex Eucommiae fat;
(2) senlciphylline preparation method;
Climbing groundsel medicinal raw material 10kg is taken, is crushed to 60 mesh, adds in 85% ethanol solution refluxing extraction 5 times, merges extracting solution, adds
Enter the aluminium oxide color of the 2% of its volume, be obtained by filtration destainer, destainer is concentrated into medicinal extract, medicinal extract with 3% citric acid water
Solution dissolves, filtering, and HD-8 cation exchange resins on filtrate are first washed with deionized water to neutrality, then with 60L, pH11's
70% ethanol solution elutes, and eluent concentration adjusts pH9.5, lets cool crystallization, crystallization, crystallization methylene chloride reflux is obtained by filtration
Dissolving, filters, lets cool crystallization, filter out crystallizing and drying up to senlciphylline while hot;
(3) high-purity L-borneol preparation method;
Step 1, natural crude product L-Borneol, L-Borneol content 63-67% are dried after centrifuge dripping；
Above-mentioned solid is dissolved in 2 times of 95% ethyl alcohol of amount by step 2；
Step 3 cools down 0.03 times of amount (weight of crude product L-Borneol) metallic sodium is added portionwise into above-mentioned solution, after finishing
When 0 DEG C of reaction 3 is small；
Step 4 is poured into above-mentioned reaction solution in 5 times of amount (weight of crude product L-Borneol) water, when standing 1.5 is small, filters；
Step 5, gained filter cake are added in 2 times of amount (weight of crude product L-Borneol) petroleum ethers, are heated to 60 DEG C, are divided sub-cloud
Water phase, organic phase are cooled to -10 DEG C, a large amount of flat crystals occur, and filtering dries, obtains high-purity L-borneol, content is
99.97%;
(4) hydroxyl cyclopentadecanone preparation method;
The first of 40ml dryings is added in into the four-neck flask of the 250ml dryings with stirring, thermometer, condenser pipe, dropping funel
Benzene, 1.60g is added in into flask, and 0.07mol metallic sodiums under nitrogen protection, are to slowly warm up to toluene boiling, quickly stir 5
Minute, sodium is broken into silvery white fine particles, temperature is controlled at 90 DEG C, is added dropwise dissolved with 4.38g (0.01mol) tetrapropyl 15
After 4 are added dropwise when small, when insulation reaction 3 is small, after reaction, the anhydrous second of 8mL is added dropwise in the 40ml toluene solutions of carbon diamides
Alcohol decomposes remaining metallic sodium, is filtered to remove insoluble matter, and filtrate water is washed till neutrality, and vacuum distillation removes solvent, obtains hydroxyl ring
15 ketone;
(5) nano silver colloidal sol preparation method;
0.5g Chinese yam starch is taken to be scattered in 10ml deionized waters, is stirred at 80 DEG C to starch solution is gelatinized to obtain, added it to
20ml concentration is in the silver ammino solution of 1wt%, is uniformly mixed and obtains starch-silver ammonia composite solution, composite solution is placed in-
Freezing processing under the conditions of 20 DEG C makees pulverization process after its icing, obtains ice powder;
100 grams of crushing of aloe, filtering take liquid, are scattered in deionized water after removing the peel, and it is 10 wt %'s to prepare 300ml concentration
Extract solution from aloe aqueous solution, potassium hydroxide solution adjust its pH to 12 and obtain alkaline extract solution from aloe；Above-mentioned ice powder is added to
In alkaline extract solution from aloe, 50 DEG C of stirring 400min obtain nano silver colloidal sol；
4. killing the stifling plant nanometer fat preparation method of air fungus infection virus according to claim 1, spy is,
10 grams of Cortex Eucommiae fat, 3 grams of senlciphylline, 8 grams of high-purity L-borneol, 3 grams of hydroxyl cyclopentadecanone, 2 grams of nano silver colloidal sol, musk deer
Fragrant 0.005 gram is incorporated in integral crushing, and 8 grams of heating of cinnamic acid are added in crushed material and are melted, is thoroughly mixed, dissolves after melting
Liquid stream enters plastic casing, obtains stifling plant nanometer fat, applied to air-conditioning, automobile, air purifier, high ferro, train, aircraft, steamer,
The uses such as container, room, school, You Eryuan, hospital, hotel, public place.
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