CN104109631A - Microalgae culture reactor - Google Patents

Microalgae culture reactor Download PDF

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Publication number
CN104109631A
CN104109631A CN201410339234.9A CN201410339234A CN104109631A CN 104109631 A CN104109631 A CN 104109631A CN 201410339234 A CN201410339234 A CN 201410339234A CN 104109631 A CN104109631 A CN 104109631A
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incubator
gas
micro
algae
culture
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CN201410339234.9A
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Chinese (zh)
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徐健
魏力
路延笃
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Qingdao Institute of Bioenergy and Bioprocess Technology of CAS
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Qingdao Institute of Bioenergy and Bioprocess Technology of CAS
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Priority to CN201410339234.9A priority Critical patent/CN104109631A/en
Publication of CN104109631A publication Critical patent/CN104109631A/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02—Photobioreactors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/22—Transparent or translucent parts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/58—Reaction vessels connected in series or in parallel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C12M37/02—Filters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Clinical Laboratory Science (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention relates to the technical field of biology, particularly a microalgae culture reactor capable of setting different concentration CO2 culture environments, which comprises a culture box, an ultraviolet lamp tube, a gas inlet pipe and a gas outlet pipe, wherein the box body of the culture box is a transparent box body; the lower side of the front end of culture box is provided with a gas inlet; the upper side of the back end of the culture box is provided with a gas outlet; the gas inlet pipe and gas outlet pipe respectively connected with the gas inlet and gas outlet of the culture box are respectively provided with a gas flowmeter and a valve; a CO2 gas analyzer for detecting carbon dioxide concentration at the gas inlet and gas outlet is arranged outside the culture box; the gas inlet and gas outlet of the culture box are respectively provided with a gas filter unit; and a humidification system is arranged in the culture box. A plurality of culture box can be arranged in parallel. By setting different concentration CO2 culture environments to screen the wild type microalgae or corresponding mutants, the complete reactor is simple in structure and convenient to operate.

Description

A kind of micro-algae is cultivated reactor
Technical field
The present invention relates to biological technical field, specifically one can arrange different concns CO 2micro-algae of culture environment is cultivated reactor.
Background technology
Micro-algae is a kind of low plant plankton such as grade spreading all over the world, and is distributed widely in the environment such as ocean, freshwater lake and light salt brine, is the important component part of primary productivity in the water globe ecosystem.Annual by the fixing CO of micro-algae photosynthesis 2account for global CO 2the more than 40% of fixed amount plays very important effect in Conversion of energy and carbon circulation, and the photosynthetic productivity of micro-algae can reach 50g/m 2/ d, is equivalent to the 10-50 of Forests carbon ability doubly, and theoretically, cultivating 1 ton of micro-algae can reducing emission of carbon dioxide approximately 1.83 tons, therefore at all reduction of discharging CO 2technology in, utilizing the photosynthesis of micro-algae to subtract carbon is tool sustainability and green friendly approach.In the time of cultivating microalgae, the residual sulphur of gas, residual nitrogen can become sulphur source and the nitrogenous source of micro algae growth, and other required nutrition of micro algae growth also can provide with nutrition waste water, reaches the object of purifying liquid waste.Therefore, develop micro-algae in China and reduce discharging CO 2technology, can reach simultaneously and " reduce CO 2discharge, substitute fossil energy, purifying exhaust air and sewage " three objects, there is wide industry and environmental applications prospect.
But micro-algae of varying environment has different fixation of C O 2ability, this be mainly because micro-algae can be grown in different CO 2under concentration environment, we need to screen and can tolerate high CO in actual applications 2micro-algae of concentration, normally 15%～20%CO 2concentration, because CO in stack gas 2concentration is the highest can reach 20%.In addition, most micro-algae has all formed a CO in long-term evolutionary process 2concentrating mechanism goes to assist to improve solid carbon ability, but this mechanism is only in air level or lower than the CO of air level 2under concentration, start, therefore in order to study CO 2concentrating mechanism, we just need to be at low CO 2under concentration, cultivate micro-algae.Under laboratory condition, in order to simulate micro-algae at high CO 2or low CO 2cultivation screening under concentration, just needs special CO 2cultivate reactor, make micro-algae in specific CO 2under concentration, grow.
At present, use more CO 2bioreactor is mainly used for the CO of plant or animal cell culture research 2incubator, wherein for the CO of animal cell culture 2incubator is not with light source conventionally, and for plant illumination CO 2incubator is generally difficult to ensure the sterile state of culturing room.In addition no matter be the CO cultivating for plant or for animal, 2incubator, their something in commons are that cost is relatively high, take up room larger, there is very high running cost, it is easy to cause the pollution of bacterium, mould etc. in actual mechanical process in addition, therefore in the correlative study of micro-algae, applies lessly, is also subject to the restriction of above factors.
Summary of the invention
The object of the present invention is to provide a kind of micro-algae to cultivate reactor, can effectively control gas flow and gas concentration lwevel, to reach, different concns CO is set 2the culture environment screening micro-algae of wild-type or corresponding mutant object, whole apparatus structure is simple, convenient operation.
The object of the invention is to be achieved through the following technical solutions:
A kind of micro-algae is cultivated reactor, comprise incubator, ultraviolet lamp tube, admission passage and outlet pipe, the admission passage being wherein connected with source of the gas is connected with the inlet mouth on incubator, outlet pipe is connected with the air outlet on incubator, be arranged at described incubator for the ultraviolet lamp tube before use incubator being carried out disinfection, on described admission passage and outlet pipe, be equipped with gas meter and valve for keeping gas concentration equilibrium in incubator.
Described incubator outside is provided with the CO that regulates or close gas meter by detecting described inlet mouth and air outlet place gas concentration lwevel 2gas analyzer.
The inlet mouth of described incubator and air outlet place are separately installed with gas-filtering device.
Described incubator front end is provided with the opening for picking and placeing the culture apparatuses such as solid plate, porous culture plate or triangular flask, and described opening is by blind flange hermetically closing.
Described ultraviolet lamp tube is arranged on described blind flange.
In described incubator, be provided with humidification system.
Described humidification system is the water pond that fills sterile pure water in.
The casing of described incubator is transparent casing, and described inlet mouth is arranged at the front end downside of described incubator, and described air outlet is arranged at the rear end upside of described incubator.
Multiple incubators are arranged in parallel by threeway is installed on admission passage.
Advantage of the present invention and positively effect are:
1, the present invention is respectively equipped with gas meter on admission passage and outlet pipe, can control as required adjustment gas concentration lwevel, and then by different concns CO is set 2the culture environment screening micro-algae of wild-type or corresponding mutant, whole apparatus structure is simple, convenient operation, and the present invention adopts the incubator of closed cultivate and screen micro-algae, has avoided the pollution of miscellaneous bacteria in air etc.
2, incubator of the present invention adopts resin material, the transparence can ensure illumination time, simultaneously, be convenient to observe algae and drop on the growing state in whole culturing process, and in incubator, be provided with ultraviolet lamp tube, be conducive to use before use uv sterilisation, avoid the pollution such as bacterium, mould in culturing process.
3, the present invention is provided with CO in incubator outside 2gas concentration analyzer is measured the gas concentration lwevel of incubator air inlet/outlet, can effectively control the gas concentration lwevel of adjusting in incubator.Pass through CO 2gas concentration analyzer is measured the CO of incubator air outlet 2concentration, needs CO when reaching 2when concentration, the gas meter that also can simultaneously close inlet mouth and air outlet maintains box house CO 2gas concentration, according to the growth cycle of different microalgae (or growth for time), carries out the displacement of 2-3 gas every day, can save in a large number like this CO 2the usage quantity of gas.
4, the present invention is provided with gas filtration bactericidal device in inlet mouth and the air outlet of incubator, the miscellaneous bacteria being used in filtering gas, avoid micro-algae in culturing process to be subject to the pollution of miscellaneous bacteria etc., and the gas filtration bactericidal device that is arranged at incubator air outlet can also prevent gas suck-back.
5, the present invention is provided with inlet mouth and air outlet along incubator diagonal, and this kind of decoration form is conducive to gas discharges after the inner abundant diffusion of incubator again, makes CO in incubator 2concentration is as far as possible consistent, has avoided the inhomogenous situation of gas concentration in reactive system.
6, the present invention is placed with humidification system in incubator, and the present invention adopts passive control wet method, in incubator, places a water pond, and interior Sheng sterile pure water relies on the spontaneous evaporation of water, makes relative humidity in casing reach 85%-95%.So also can prevent from screening for solid plate time, the solid agar on flat board is dried up prematurely.
7, the present invention realizes multiple incubators and is arranged in parallel by threeway is installed on admission passage, for Large-scale Screening.
Brief description of the drawings
Fig. 1 is structural representation of the present invention.
Wherein, 1 is gas meter; 2 is valve; 3 is blind flange; 4 is ultraviolet lamp switch; 5 is inlet mouth; 6 is air outlet; 7 is incubator; 8 is ultraviolet lamp tube; 9 is admission passage; 10 is outlet pipe.
Embodiment
Below in conjunction with accompanying drawing, the invention will be further described.
As shown in Figure 1, the present invention includes incubator 7, ultraviolet lamp tube 8, admission passage 9 and outlet pipe 10, wherein be provided with inlet mouth 5 at the front end downside of incubator 7, in the rear end of incubator 7, upside is provided with air outlet 6, admission passage 9 is connected with the inlet mouth 5 on incubator 7, outlet pipe 10 is connected with the air outlet 6 on incubator 7, described inlet mouth 5 and air outlet 6 are arranged at respectively the front end downside of incubator 7 and rear end upside and are conducive to gas and fully after diffusion, discharge incubator 7 is inner again, make the interior gas concentration of incubator 7 even.Ultraviolet lamp tube 8 is arranged in described incubator 7, for before use incubator 7 being carried out to uv sterilisation, prevents the pollution such as bacterium, mould, and described ultraviolet lamp tube 8 is controlled open and close by the ultraviolet lamp switch 4 that is arranged on incubator 7 outsides.Be provided with the opening for picking and placeing the culture apparatuses such as solid plate, porous plate or triangular flask at incubator 7 front ends, described opening is by blind flange 3 hermetically closings, ultraviolet lamp tube 8 is arranged on described blind flange 3, and when blind flange 3 is opened, ultraviolet lamp tube 8 can move with described blind flange 3.Described incubator 7 is made up of light transmissive material, and in the present embodiment, described incubator 7 is made up of transparent resin material, and transparence can reach 90%.
On admission passage 9 and outlet pipe 10, be respectively equipped with gas meter 1 and the pilot piping keying of controlling gas flow rate and the valve 2 of adjusting gas flow, in the present embodiment, the model of described gas meter 1 is red flag LZB-10, manufacturer is Changzhou double wave instrument company limited, is provided with for detection of the inlet mouth 5 of described incubator 7 and the CO of air outlet 6 place's gas concentration lwevels in incubator 7 outsides 2gas analyzer, in the time that the gas concentration lwevel in incubator 7 does not meet the demands, described CO 2gas analyzer can send Signal Regulation gas meter 1, and the gas concentration lwevel that prevents gas concentration lwevel in incubator 7 and pass into gas has big difference, and ensures that micro-algae cultivates under default gas concentration lwevel, as the air outlet of described incubator 76 CO of place 2when concentration reaches the concentration needing, described CO 2gas analyzer also can close simultaneously on admission passage 9 and outlet pipe 10 gas meter 1 carry out the CO of maintain case 7 inside 2gas concentration, then, according to the growth cycle of different microalgae (or growth for time), carries out the displacement of 2～3 gases every day, can save in a large number like this CO 2the usage quantity of gas.In the present embodiment, described CO 2the model of gas analyzer is C150 type CO 2concentration analyzer, manufacturer is Geotech company of Britain.
Inlet mouth 5 and 6 places, air outlet at incubator 7 are respectively equipped with gas-filtering device, wherein be arranged on the gas-filtering device at inlet mouth 5 places for filtration sterilization, the gas-filtering device that is arranged on 6 places, air outlet is filtering simultaneously also for preventing that extraneous gas suck-back from entering incubator 7, in the present embodiment, the gas-filtering device that is arranged on inlet mouth 5 places of incubator 7 is millipore filtration, utilize filtering with microporous membrane gas for techniques well known, the gas-filtering device that is arranged on 6 places, air outlet of incubator 7 is air filter, described air filter model is Sartorius Midisart200017805, manufacturer is German Sartorius/ Sai Duolisi company.
Source of the gas communicates with the inside of incubator 7 by admission passage 9, described source of the gas is air pump (for pass into pressurized air in incubator 7) or gas cylinder (for passing in advance and configure gas by different ratios in incubator 7), and the flow of gas and flow velocity can regulate by the gas meter 1 on pipeline and valve 2.The present invention also can arrange independent pure CO 2gas cylinder pipeline and pneumatic line, be connected by a threeway above-mentioned two gas pipings with a gas buffer bottle, gas buffer bottle is connected with incubator 7 by pipeline again, at described pure CO 2gas meter adjustments of gas flow velocity is set respectively on gas cylinder pipeline and pneumatic line, and gas buffer bottle is for premixed gas.
In incubator 7, be provided with humidification system, because except ensureing in incubator 7 CO accurately 2outside content, stable and accurate humidity is also an important indicator of desirable physiological environment, and the present invention adopts passive control wet method, at the water pond of one of the interior placement of incubator 7, interior Sheng sterile pure water, relies on the spontaneous evaporation of water, makes relative humidity in casing reach 85%-95%.So also can prevent from screening for solid plate time, the solid agar on flat board is dried up prematurely.The present embodiment is in capacity 1L water pond of the interior placement of incubator 7, interior Sheng 0.5L sterile pure water.
Principle of work of the present invention is:
The present invention before use, can first carry out disinfection by built-in ultraviolet lamp tube 8, sterilize after 3 hours, close ultraviolet lamp tube 8, then open blind flange 3, put into coated in advance solid plate or liquid culture porous plate (24 holes or 96 holes), put into simultaneously the water pond of sterilizing pure water is housed, then close blind flange 3 tighten the screws.Source of the gas is by admission passage 9 input airs and carbon dioxide mix gas, Ventilation Rate is measured by the gas meter 1 on admission passage 9, and by valve 2 adjust fluxes on admission passage 9, gas enters in incubator 7 after the gas-filtering device at inlet mouth 5 places filters, simultaneously valve 2 and the gas meter 1 on outlet pipe 10 also carried out corresponding adjusting, to ensure the constant and steady air current of the interior gas concentration lwevel of incubator 7.
Incubator 7 outsides are provided with CO 2concentration analyzer, for measuring respectively the gas concentration of carbonic acid gas of inlet and outlet incubator 7, also can measure O in incubator 2concentration and relative humidity, and by measuring the concentration of gas port 6 carbonic acid gas, regulate the gas meter 1 on admission passage 9 and outlet pipe 10, to ensure that micro-algae cultivates under default gas concentration lwevel, and ensures that relative humidity is constant.
Fall when grow to enough large algaes at micro-algae solid plate, naked eyes are visible, or when on liquid culture 24 orifice plates, (or in triangular flask) naked eyes can obviously be seen green, first close gas source valve, then close the valve 2 on admission passage 9, then open blind flange 3, take out solid plate sample or porous plate liquid culture sample, select interested algae and drop into row enlarged culturing, carry out the research of other biological aspect.
The present invention can also realize multiple incubators 7 and is arranged in parallel by threeway is installed on admission passage 9, for Large-scale Screening.

Claims (9)

1. a micro-algae is cultivated reactor, it is characterized in that: comprise incubator (7), ultraviolet lamp tube (8), admission passage (9) and outlet pipe (10), the admission passage (9) being wherein connected with source of the gas is connected with the inlet mouth (5) on incubator (7), outlet pipe (10) is connected with the air outlet (6) on incubator (7), be arranged at described incubator (7) for the ultraviolet lamp tube (8) before use incubator (7) being carried out disinfection, on described admission passage (9) and outlet pipe (10), be equipped with gas meter (1) and valve (2) for keeping gas concentration equilibrium in incubator (7).
2. micro-algae according to claim 1 is cultivated reactor, it is characterized in that: described incubator (7) outside is provided with the CO that locates gas concentration lwevel and regulate or close gas meter (1) by detecting described inlet mouth (5) and air outlet (6) 2gas analyzer.
3. micro-algae according to claim 1 and 2 is cultivated reactor, it is characterized in that: gas-filtering device is located to be separately installed with in inlet mouth (5) and air outlet (6) of described incubator (7).
4. micro-algae according to claim 1 is cultivated reactor, it is characterized in that: described incubator (7) front end is provided with the opening for picking and placeing the culture apparatuses such as solid plate, porous culture plate or triangular flask, described opening is by blind flange (3) hermetically closing.
5. micro-algae according to claim 4 is cultivated reactor, it is characterized in that: described ultraviolet lamp tube (8) is arranged on described blind flange (3).
6. micro-algae according to claim 1 is cultivated reactor, it is characterized in that: described incubator is provided with humidification system in (7).
7. micro-algae according to claim 7 is cultivated reactor, it is characterized in that: described humidification system is the water pond that fills sterile pure water in.
8. micro-algae according to claim 1 is cultivated reactor, it is characterized in that: the casing of described incubator (7) is transparent casing, described inlet mouth (5) is arranged at the front end downside of described incubator (7), and described air outlet (6) are arranged at the rear end upside of described incubator (7).
9. micro-algae according to claim 1 is cultivated reactor, it is characterized in that: multiple incubators (7) are by being arranged in parallel in the upper installation of admission passage (9) threeway.
CN201410339234.9A 2014-07-16 2014-07-16 Microalgae culture reactor Pending CN104109631A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106119106A (en) * 2016-06-29 2016-11-16 哈尔滨华藻生物科技开发有限公司 Device is cleaned in a kind of spirulina sterilization
CN106706909A (en) * 2016-12-26 2017-05-24 中国科学院水生生物研究所 Method and device applied to assessment of carbon dioxide tolerance of microalgae
CN113265317A (en) * 2021-05-31 2021-08-17 浙江省海洋水产养殖研究所 Microalgae culture equipment
CN116463197A (en) * 2023-06-19 2023-07-21 愚公生态科技股份有限公司 Carbon fixation photobioreactor and application thereof

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2394915Y (en) * 1999-08-02 2000-09-06 中国科学院化工冶金研究所 Bubble tower type light biological reactor
CN1635107A (en) * 2004-11-11 2005-07-06 上海交通大学 Large-scale seaweed cell or tissue reactor pre-culturing system
CN1680539A (en) * 2005-01-26 2005-10-12 湖北师范学院 Pneumatically liftable photo-biologic reactor for high density culture of algae
CN102533528A (en) * 2012-01-12 2012-07-04 天津大学 Sealed continuous culture experimental apparatus based on simulation of microalgae amplification culture
CN102703326A (en) * 2012-02-13 2012-10-03 青岛理工大学 Microalga strain with high CO2 tolerance and high fixation rate and breeding method thereof
CN202595126U (en) * 2012-05-22 2012-12-12 中国水产科学研究院东海水产研究所 Primary culture device of microalgae
CN203187688U (en) * 2013-04-24 2013-09-11 重庆大学 Algae cultivation box
CN203238266U (en) * 2013-05-13 2013-10-16 宁夏大学 Photobioreactor device for culturing microalgae in high density
CN103773673A (en) * 2014-02-25 2014-05-07 中国科学院水生生物研究所 Cylindrical air-lift type high-efficient photobioreactor for microalgae culture and application thereof

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2394915Y (en) * 1999-08-02 2000-09-06 中国科学院化工冶金研究所 Bubble tower type light biological reactor
CN1635107A (en) * 2004-11-11 2005-07-06 上海交通大学 Large-scale seaweed cell or tissue reactor pre-culturing system
CN1680539A (en) * 2005-01-26 2005-10-12 湖北师范学院 Pneumatically liftable photo-biologic reactor for high density culture of algae
CN102533528A (en) * 2012-01-12 2012-07-04 天津大学 Sealed continuous culture experimental apparatus based on simulation of microalgae amplification culture
CN102703326A (en) * 2012-02-13 2012-10-03 青岛理工大学 Microalga strain with high CO2 tolerance and high fixation rate and breeding method thereof
CN202595126U (en) * 2012-05-22 2012-12-12 中国水产科学研究院东海水产研究所 Primary culture device of microalgae
CN203187688U (en) * 2013-04-24 2013-09-11 重庆大学 Algae cultivation box
CN203238266U (en) * 2013-05-13 2013-10-16 宁夏大学 Photobioreactor device for culturing microalgae in high density
CN103773673A (en) * 2014-02-25 2014-05-07 中国科学院水生生物研究所 Cylindrical air-lift type high-efficient photobioreactor for microalgae culture and application thereof

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106119106A (en) * 2016-06-29 2016-11-16 哈尔滨华藻生物科技开发有限公司 Device is cleaned in a kind of spirulina sterilization
CN106706909A (en) * 2016-12-26 2017-05-24 中国科学院水生生物研究所 Method and device applied to assessment of carbon dioxide tolerance of microalgae
CN106706909B (en) * 2016-12-26 2019-04-19 中国科学院水生生物研究所 A kind of method and device applied to assessment microalgae carbon dioxide tolerance
CN113265317A (en) * 2021-05-31 2021-08-17 浙江省海洋水产养殖研究所 Microalgae culture equipment
CN116463197A (en) * 2023-06-19 2023-07-21 愚公生态科技股份有限公司 Carbon fixation photobioreactor and application thereof
CN116463197B (en) * 2023-06-19 2024-02-02 愚公生态科技股份有限公司 Carbon fixation photobioreactor and application thereof

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Application publication date: 20141022