CN2394915Y - Bubble tower type light biological reactor - Google Patents
Bubble tower type light biological reactor Download PDFInfo
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- CN2394915Y CN2394915Y CN 99217362 CN99217362U CN2394915Y CN 2394915 Y CN2394915 Y CN 2394915Y CN 99217362 CN99217362 CN 99217362 CN 99217362 U CN99217362 U CN 99217362U CN 2394915 Y CN2394915 Y CN 2394915Y
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M31/00—Means for providing, directing, scattering or concentrating light
- C12M31/02—Means for providing, directing, scattering or concentrating light located outside the reactor
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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Abstract
The utility model relates to a bubble tower type light biological reactor, which belongs to the technical field of micro algae culturing light biological reactors. A bottom plate of the reactor is provided with a platform, guard posts and light sources are arranged around the platform, and the light sources are connected with a time controller; a tank body of the reactor is arranged on the platform, the bottom of the tank body is provided with a temperature control interlayer, and an air inlet pipe and a carbon dioxide gas inlet pipe respectively enter an air distributing container; a mix air outlet pipe extends out from the air distributing container and is connected with a filter, and is connected with an air distributor which is arranged on the bottom of the tank body. The utility model has the higher light receiving area and volume ratio, and can not generate the oxygen accumulation. The utility model has the characteristics of effective disinfection, clear fluid mechanics behaviours, etc.
Description
The utility model belongs to little algae and cultivates bioreactor technical field, the particularly tower bioreactor of bubbling.
Present little algae is cultivated reactor and divides two kinds of open type and closeds, and its various reactor design are mainly pursued higher light-receiving area/volume ratio and eliminated problem such as oxygen accumulation.Closed bioreactor kind is more, mainly contain several forms such as tubular type, flat and pillar, and use comparatively extensive with tubular reactor, compare with the open type reactor, closed has and is easy to guarantee that gnotobasis, culture condition are easy to control, can realize advantage such as high-cell-density cultivation.But they often also have the deficiency of oneself, most of reactors since material and reasons in structure can not thoroughly sterilize, strict for aseptic condition, be not suitable for as the little algae mixotrophism culturing process that adds organic substrates; Quite most reactor inner fluid mechanical behaviors are clear inadequately; The perhaps biotechnology of producing: outdoor large scale culturing systematic review " using the phycology magazine " 5:593-604 (Chaumont D (1993) Biotechnology of algal biomass production:a review ofsystems for outdoor mass culture.J.Appl.Phycol.5:593-604) as Chaumont D (1993) algae biomass; With Tredici MR, Materassi R (1992) is from open pond to vertical caulking groove plate: experience " use phycology magazine " 4:221-231 (the Tredici MR of Italy in the exploitation of phototroph microorganism large scale culturing reactor, Materassi R (1992) From open ponds to vertical alveolar panels:the Italian experience in the development ofreactors for the mass cultivation of phototrophic microorganisms.J.Appl.Phycol.4:221-231) pointed a lot of temperature of reactor controls are not ideal enough, culturing process algae liquid often has temperature rising phenomenon, has had a strong impact on the growth of frustule.From Javanmardian M, the high-density light autotrophy of Palsson BO (1991) algae is cultivated: a kind of design of novel photo-bioreactor system, make and operation " biotechnology and biotechnology " 38:1182-1189 (Javanmardian M, Palsson BO (1991) High-densityphotoautotrophic algal cultures:design, constrution, and operation of a novelphotobioreactor system.Biotechnol.Bioeng.38:1182-1189.) though can find out that also some reactor technical indicator is preferable, big and photosynthesis rate is higher as light-receiving area/volume ratio, but complex structure, the cost of manufacture height; Some reactor is bigger for the pursuit area/volume ratio takes up room, fitting operation inconvenience.
The purpose of this utility model is to provide a kind of small-sized bubbling tower bioreactor, it has higher area/volume ratio and is not producing on the basis of oxygen accumulation, reactor can thoroughly be sterilized, and reactor inner fluid mechanical behavior is clear, has the growing environment of good frustule; And device structure is simple, and fitting operation is convenient, and reactor takes up room little, and equipment cost is low.
The technical solution of the utility model is to realize like this.Comprise bioreactor tank body, light source and CO
2Air distributing device is characterized in that: a platform 20 is arranged on reactor base plate 19, and symmetry is erect around platform 20 guard post 21 and light source 22, and light source 22 all links to each other with time controller 23 by lead; Bubbling column reactor tank body 1 is located on the platform 20, and the tank body top is provided with inoculation mouth 7, thermometer mouth 8, electrode ports 9, air outlet 5 and thief hole 6; One temperature control interlayer 3 is arranged at tank body 1 bottom, and the both sides of temperature control interlayer 3 have circulating water intake 10 and circulating water outlet 11 respectively; Air intake duct 13 links to each other with an end of gas meter 15, and the gas piping that the other end of gas meter 15 connects stretches in the distribution container 12; CO
2Inlet pipe 14 links to each other with an end of gas meter 16, and the gas piping that the other end of gas meter 16 connects stretches in the distribution container 12; Gas mixture escape pipe 17 stretches out from distribution container 12, links to each other with an end of strainer 18, and the other end of strainer 18 links to each other with inlet pipe 4, and inlet pipe 4 links to each other with the glass sand core gas distributor 2 that is positioned at tank base central authorities.
Wherein, inoculation mouthful 7 plug central authorities are a thermometer mouth 8, and thermometer can be placed in one.Electrode ports 9 is inserted with pH electrode or DO electrode.The reactor height is 1.5～4.0 with diameter ratio, and gas distributor and reactor diameter ratio are 0 25～0.50, and reactor light-receiving area and volume ratio are 30～60m
-1Light source adopts commercially available different capacity daylight type electricity-saving lamp, controls automatically by the different steps setting intensity of illumination that time controller is cultivated according to little algae.
The utility model bioreactor is by bubbling tower type reactor, light source and CO
2Gas distributing system three parts are formed, and three parts can freely separate and make up.The Lights section comprises light source and time controller, and light source is looped around around the reactor, is fixed in above the mounting, and the quantity of light source and position can freely change, but light intensity so free adjustment, and the light intensity cycle can be by the further control of time controller do.CO
2Gas distributing system can pass through air and CO
2The different set of flow makes the air that feeds reactor be rich in the CO of different concns
2, satisfy the demand of little algae to carbon source.Reactor is that material is made with the hard glass, can utilize easy glass processing technology blowing to form, and need not use hardware except that the thermometer intubate.The needs of sterilization have been considered in the geometrical shape of reactor and volume design, can put into common laboratory and carry out the steam moist heat sterilization with small-sized Autoclave, have avoided the not thorough phenomenon of bringing with the chemical process sterilization.Reactor adopts cylindric bubble tower structure, and gas enters tank body from the top, and processing, arrangement and operation are all very convenient, and gas distributor is the disc glass sand core, is positioned at bottom center.Reactor internal components is less, and bubble is evenly distributed, and gas-liquid mixed and mass transfer effect are good, and whole culture system shearing action is lower, helps the growth of microalgae cell, and the hydromeehanics behavior in the reactor has the essential characteristic of bubble tower.Because the homodisperse of gas and effectively separates the phenomenon that the difficult generation of culturing process oxygen accumulates.Reactor bottom has been done a temperature control interlayer, the turnover that utilizes thermostatical circulating water is carried out temperature control to reactor, has avoided wall chuck heat preserving method to the considerable influence that is subjected to light in the reactor and bigger difficulty of processing.Because heat exchange area is bigger, stable temperature control has been eliminated recurrent culture-liquid temp rising phenomenon in little algae cultivation, has overcome the sufficient inadequately weakness of mode temperature controls such as electrically heated rod and recirculated water intubate.Culturing process sampling is taken out by the thief hole at top (claiming discharge gate when the discharging) by differential pressure action, when reactor during as the preposition seed culture device of little algae amplification culture, can easily algae hydraulic pressure be gone into the next stage bioreactor by thief hole simultaneously.The whole reactor system architecture is simple, and handling ease is with low cost.Because reactor bottom does not have port, this reactor can place on arbitrary table top, and is flexible and convenient to use.
The tower bioreactor of small-sized bubbling of the present utility model is carrying out little algae when cultivating, because reactor can thoroughly sterilize, though culture condition gentleness (pH is shown as neutrality) also can keep cultivating the pollution-free state of whole process; The reactor fluid movement shows very strong regularity, and shearing action is less, and when cultivating the filamentous algae cell, microscopically is observed algal filament all to exist than long status; Because reactor bottom has bigger heat exchange area, temperature control is very stable, and temperature variation generally is no more than set(ting)value+1 ℃, has overcome temperature fluctuation having a strong impact on the frustule growth.Satisfy conditions such as the big and difficult generation oxygen of light-receiving area/volume ratio accumulation simultaneously owing to reactor, this reactor adapted is carried out the cultivation of various thread little algaes and unicellular little algae, and all can reach higher cell density.Simultaneously, this reactor is in operation, and each links such as sterilization, inoculation, sampling, cleaning and dismounting are all very convenient.
Below in conjunction with drawings and Examples the technical solution of the utility model is further described.
Fig. 1. the utility model tank body and CO
2The structural representation of distribution part;
Fig. 2. the structural representation of the utility model the Lights section;
Fig. 3. the structural representation after the utility model assembling.
Number in the figure:
1. tank body 2. gas distributors 3. temperature control interlayers 4. inlet pipe
5. air outlet 6. thief holes or discharge gate 7. are inoculated mouthful 8. thermometer mouths
9. electrode ports 10. circulating water intakes 11. circulating water outlets 12. distribution containers
13. air intake duct 14.CO
2Inlet pipe 15. gas meters 16. gas meters
17. gas mixture escape pipe 18. strainers 19. reactor base plates 20. platforms
21. guard post 22. light sources 23. time controllers
Embodiment 1.
Little algae of cultivating is a thread fixed nitrogen blue-green algae anabena 7120 (Anabaena sp.PCC7120), shakes liquid cultivation of bottle kind and reactor cultivation and all adopts blue-green algae to cultivate general BG-11 substratum (pH7.5).One cumulative volume is the tower bioreactor of the bubbling of 1.8L, its working volume is 1.5L, height for reactor is 200mm, one platform 20 is arranged on reactor base plate 19, the diameter that is located in tank body 1 thereon is 120mm, the symmetry setting has guard post 21 and light source 22 around 19 the platform 20 on base plate, and reactor light source 22 is provided by 4 18W daylight type electricity-saving lamps, and it all links to each other with time controller 23 by lead; Being provided with inoculation mouth 7 at the tank body top, is a stainless steel intubate in its plug central authorities, and it is that thermometer mouth 8, one thermometers are placed in one, and electrode ports 9 is inserted a DO electrode, also is provided with air outlet 5 and thief hole 6 at the tank body top; One temperature control interlayer 3 is arranged at tank body 1 bottom, and its height is 20mm, and the both sides of temperature control interlayer 3 have circulating water intake 10 and circulating water outlet 11 respectively; Air intake duct 13 links to each other with an end of gas meter 15, and the gas piping that the other end of gas meter 15 connects stretches in the distribution container 12; CO
2Inlet pipe 14 links to each other with an end of gas meter 16, and the gas piping that the other end of gas meter 16 connects stretches in the distribution container 12; Gas mixture escape pipe 17 stretches out from distribution container 12, link to each other with an end of strainer 18, the other end of strainer 18 links to each other with inlet pipe 4, and inlet pipe 4 links to each other with the glass sand core gas distributor 2 that is positioned at tank base central authorities, its diameter is 40mm, and the glass sand core mean pore size is 80 μ m; It is hard glass B40 that reactor is made material.
The substratum that reactor is cultivated usefulness joins in the reactor tank body 1 by inoculation mouth 7, and substratum is put into the Autoclave sterilization with tank body, and kind liquid is cultivated and carried out on rotary shaking table, and shaking speed is 130rpm, and temperature is 32 ℃, and light intensity is 160 μ Em
-2s
-1, cultivate after 6 days, on Bechtop, cross the inoculation mouthful tower bioreactor of 7 access bubblings in the flame bypass, inoculum size is 20%.The reactor culture condition is: 32 ℃ of temperature, air flow (air) 0.178vvm, surperficial largest light intensity 624 μ Em
-2s
-1, minimum intensity of light 110 μ Em
-2s
-1Reactor is cultivated cell growth in 10 days and is reached stationary phase, and maximum cell density is 0.89g/L, and the logarithmic phase specific cell growth rate is 0.30d
-1It is all longer that microscopically is observed algal filament, and cellular form is full, does not have living contaminants.Cultivation finishes the back and discharges nutrient solution by inoculation mouth 7, discharge gate 6 etc., and cleans.
Claims (4)
1. the tower bioreactor of bubbling comprises bioreactor tank body, light source and CO
2Air distributing device is characterized in that: a platform (20) is arranged on reactor base plate (19), in platform (20) symmetry setting on every side guard post (21) and light source (22) are arranged, light source (22) all links to each other with time controller (23) by lead; Bubbling column reactor tank body (1) is located on the platform (20), and the tank body top is provided with inoculation mouthful (7), thermometer mouth (8), electrode ports (9), air outlet (5) and thief hole (6); One temperature control interlayer (3) is arranged at tank body (1) bottom, and the both sides of temperature control interlayer (3) have circulating water intake (10) and circulating water outlet (11) respectively; Air intake duct (13) links to each other with an end of gas meter (15), and the gas piping that the other end of gas meter (15) connects stretches in the distribution container (12); CO
2Inlet pipe (14) links to each other with an end of gas meter (16), and the gas piping that the other end of gas meter (16) connects stretches in the distribution container (12); Gas mixture escape pipe (17) stretches out from distribution container (12), links to each other with an end of strainer (18), and the other end of strainer (18) links to each other with inlet pipe (4), and inlet pipe (4) links to each other with the glass sand core gas distributor (2) that is positioned at tank base central authorities.
2. the tower bioreactor of bubbling as claimed in claim 1 is characterized in that described inoculation mouthful (7) plug central authorities are a thermometer mouth (8).
3. the tower bioreactor of bubbling as claimed in claim 1 is characterized in that described electrode ports (9) is inserted with pH electrode or DO electrode.
4. the tower bioreactor of bubbling as claimed in claim 1 is characterized in that described reactor height is 1.5～4.0 with diameter ratio, and gas distributor and reactor diameter ratio are 0.25～0.50, and reactor light-receiving area and volume ratio are 30～60m
-1
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 99217362 CN2394915Y (en) | 1999-08-02 | 1999-08-02 | Bubble tower type light biological reactor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 99217362 CN2394915Y (en) | 1999-08-02 | 1999-08-02 | Bubble tower type light biological reactor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2394915Y true CN2394915Y (en) | 2000-09-06 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 99217362 Expired - Fee Related CN2394915Y (en) | 1999-08-02 | 1999-08-02 | Bubble tower type light biological reactor |
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| Country | Link |
|---|---|
| CN (1) | CN2394915Y (en) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101942383A (en) * | 2010-08-05 | 2011-01-12 | 南京工业大学 | Photo-bioreactor |
| CN101643699B (en) * | 2009-06-22 | 2012-07-04 | 新奥科技发展有限公司 | System for cultivating microalgae with waste water |
| CN102577922A (en) * | 2011-12-30 | 2012-07-18 | 北京工业大学 | Submerged plant and algae coculture equipment |
| CN101700469B (en) * | 2009-12-02 | 2012-08-08 | 天津大学 | Apparatus and method of bubble column three-phase bio-reactor for purifying toluene waste gas |
| CN102757894A (en) * | 2012-07-31 | 2012-10-31 | 济南大学 | Inverted conical microporous bubbling liquid-phase submerged fermentation device |
| CN102824882A (en) * | 2012-07-31 | 2012-12-19 | 济南大学 | Circular inverted cone type bubbling stirred tank reactor |
| CN104109631A (en) * | 2014-07-16 | 2014-10-22 | 中国科学院青岛生物能源与过程研究所 | Microalgae culture reactor |
| CN105143430A (en) * | 2013-04-22 | 2015-12-09 | 费尔曼塔格公司 | Reactor with integrated illumination |
| CN114210283A (en) * | 2022-01-25 | 2022-03-22 | 上海交通大学 | Photoreactor |
-
1999
- 1999-08-02 CN CN 99217362 patent/CN2394915Y/en not_active Expired - Fee Related
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101643699B (en) * | 2009-06-22 | 2012-07-04 | 新奥科技发展有限公司 | System for cultivating microalgae with waste water |
| CN101700469B (en) * | 2009-12-02 | 2012-08-08 | 天津大学 | Apparatus and method of bubble column three-phase bio-reactor for purifying toluene waste gas |
| CN101942383A (en) * | 2010-08-05 | 2011-01-12 | 南京工业大学 | Photo-bioreactor |
| CN101942383B (en) * | 2010-08-05 | 2013-04-10 | 南京工业大学 | Photo-bioreactor |
| CN102577922A (en) * | 2011-12-30 | 2012-07-18 | 北京工业大学 | Submerged plant and algae coculture equipment |
| CN102757894A (en) * | 2012-07-31 | 2012-10-31 | 济南大学 | Inverted conical microporous bubbling liquid-phase submerged fermentation device |
| CN102824882A (en) * | 2012-07-31 | 2012-12-19 | 济南大学 | Circular inverted cone type bubbling stirred tank reactor |
| CN105143430A (en) * | 2013-04-22 | 2015-12-09 | 费尔曼塔格公司 | Reactor with integrated illumination |
| CN104109631A (en) * | 2014-07-16 | 2014-10-22 | 中国科学院青岛生物能源与过程研究所 | Microalgae culture reactor |
| CN114210283A (en) * | 2022-01-25 | 2022-03-22 | 上海交通大学 | Photoreactor |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |