CN201605272U - Photosynthetic bacterium cultivating pot - Google Patents

Photosynthetic bacterium cultivating pot Download PDF

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Publication number
CN201605272U
CN201605272U CN 201029092045 CN201029092045U CN201605272U CN 201605272 U CN201605272 U CN 201605272U CN 201029092045 CN201029092045 CN 201029092045 CN 201029092045 U CN201029092045 U CN 201029092045U CN 201605272 U CN201605272 U CN 201605272U
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CN
China
Prior art keywords
tank
pot
photosynthetic bacterium
bacterium culture
interlayer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN 201029092045
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Chinese (zh)
Inventor
杨建设
姜仕
马文跃
杨喜军
杨策军
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
HUNAN HUINONG TECHNOLOGY CO., LTD.
Original Assignee
Hunan Benzhi Biotechnology Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hunan Benzhi Biotechnology Co Ltd filed Critical Hunan Benzhi Biotechnology Co Ltd
Priority to CN 201029092045 priority Critical patent/CN201605272U/en
Application granted granted Critical
Publication of CN201605272U publication Critical patent/CN201605272U/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02Photobioreactors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/34Internal compartments or partitions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02Stirrer or mobile mixing elements
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature

Abstract

The utility model discloses a photosynthetic bacterium cultivating pot. The pot body adopts the interlayer structure; a cooling or heating medium inlet and a cooling or heating medium outlet are formed on the interlayer; a temperature regulation control device is arranged on the pot body; one to ten lighting lamp tubes with wavelengths of 450 to 550 microns are uniformly distributed on the inner cavity of the pot body; an air inlet pipe extending into the bottom of the inner cavity of the pot body from the top of the pot body is arranged on the pot body; and a stirring device is mounted at the bottom of the pot body. The utility model can meet the requirement for energy of light of photosynthetic bacterium, can control the temperature of culture solution in the pot within the optimum bacterium cultivating temperature, create conditions for bacterium purifying and cultivating since air outside the pot is prevented from entering, can be applicable to mass production, and have a scientific and reasonable structure, and can promote microbiological products good for human to be widely developed and applied.

Description

The photosynthetic bacterium culture tank
Technical field
The utility model relates to a kind of photosynthetic bacterium culture tank, particularly a kind of light culture tank that is applicable to multiple photosynthetic bacterium mixed culture.
Background technology
Photosynthetic bacterium (English name: Photosynthetic Bacteria Abbr.name:PSB) be occur on the earth the earliest, occurring in nature ubiquity, prokaryotic organism with original luminous energy synthetic system, it is the general name of can under the anaerobism illumination condition, not put the oxygen photosynthetic bacteria, be the Gram-negative bacteria that a class does not form the gemma ability, being a class carries out photosynthetic microorganism as the energy, the organism that can utilize occurring in nature under anaerobism illumination or aerobic dark condition, sulfide, ammonia etc. as the hydrogen donor carbon source of holding concurrently with light.Photosynthetic bacterium can absorb the 450-550 mum wavelength light of suitable growth, and suitable water temperature is 15 ℃-40 ℃, and optimum water temperature is 28 ℃-36 ℃.In aquaculture, toxic substances such as the nitrite in the water body of can degrading, sulfide, realize serving as bait, purify water, preventing disease, as functions such as fodder additivess.Agricultural planting is rich soil and the main strength that promotes animal and plant growth, and foliage-spray promotes photosynthesis.Photosynthetic bacterium adaptability is strong, can restrain oneself the organic waste water of high depth and stronger decomposition and inversion ability, poisonous substances such as phenol, cyanogen are had necessarily stand and characteristics such as capacity of decomposition, its many characteristics make it have huge using value in nuisanceless aquaculture and agricultural planting.
Present Research shows both at home and abroad, and the photosynthetic bacterium training method mainly contains dynamically and leave standstill 2 kinds of modes at present, and culture apparatus has open and airtight 2 types.The report that the dynamic cultured continuously photosynthetic bacterium of closed bioreactor of adopting printing opacity is abroad arranged, but domestic glass jar and the plastic tank of adopting leaves standstill airtight cultivation more under illumination.Dynamic airtight cultivation is generally carried out in the reactor of printing opacity, can access sufficient illumination in order to keep culture, makes the photosynthetic bacterium liquid culture flow through the Glass tubing of spiral printing opacity by pump.Generally being divided into level Four for the scale production technology that leaves standstill sealing cultivation photosynthetic bacterium and cultivating, is respectively the switching and the purifying of one-level test tube kind; The secondary Erlenmeyer flask is cultivated, 1~5 liter of volume of culture; Three grades of enlarged culturing, 25~50 liters of volume of culture; Level Four enlarged culturing, volume of culture are 100~500 liters.Though it is a variety of that the production method of photosynthetic bacterium has, adopt glass jar and plastic tank to leave standstill airtight small dimension training method under illumination but be limited to up to the present, how the large scale culturing photosynthetic bacterium is the restriction photosynthetic bacterium is cultivated and the bottleneck problem of using.
The utility model content
The purpose of this utility model is to overcome the deficiencies in the prior art, and one kind of multiple photosynthetic bacterium mixed culture jars are provided, and to realize being applied to large-scale production, promotion develops widely human beneficial microorganism product and uses.
Technical scheme of the present utility model is: described tank body is an interlayer, cold and hot matchmaker's import and outlet are housed on interlayer, on tank body, be provided with the temperature regulation control device, the illuminating lamp tube that 1-10 wavelength is 450~550 μ m is distributed on tank inner chamber, on tank body, be provided with the inlet pipe that extends to the tank inner chamber bottom from the tank body top, whipping appts be housed in tank base.
Described fluorescent tube is 6 fluorescent lamps or incandescent light, and 6 fluorescent tubes are contained in 6 clear lamp sockets,, axial array circumferentially even along tank body.
The respiratory organ interface that stops jar outer air to enter is equipped with on described tank body top.
The volume of described tank body is 1000~5000 liters.
Be equipped with 1-4 on the described tank wall and observe visor.
Described tank body top and/or sidepiece are provided with access hole.
The utility model is by satisfying the needs of photosynthetic bacterium to luminous energy to the control of fluorescent tube quantity and intensity; And remain in the best microbial culture temperature by the temperature of nutrient solution in the temperature regulation control device controlling tank; Be provided with the respiratory organ interface at the tank body top and can stop jar outer air admission, for the condition that provides of cultivating is provided bacterium, tank base the nutriment that whipping appts adds in can be with culturing process is housed or sink to bottom the part thalline stir; Tank body is provided with access opening, for maintenance, cleaning and sterilization are provided convenience, the tank body cubic capacity is 1000~5000 liters, applicable in large-scale production, and scientific structure, rationally, facility investment is not high, energy consumption is low, can promote human beneficial microorganism product is developed widely and uses.
Description of drawings
Fig. 1 is embodiment 1 an overall structure synoptic diagram;
Fig. 2 is the vertical view of Fig. 1.
Embodiment
The utility model is described in further detail below in conjunction with the drawings and specific embodiments.
Embodiment 1:
With reference to accompanying drawing: the tank body 3 of present embodiment is that a useful volume is 2000 liters a stainless steel tank body, tank body 3 is provided with the clear lamp socket 4,4.1,4.2,4.3,4.4,4.5 that extends to tank body 3 inner chambers and amounts to six, transparent socket adopts glass to make, and, axial array circumferentially even along tank body 2.Many incandescent light or fluorescent tube that photosynthetic bacterium can absorb the 450-550 mum wavelength of suitable growth are installed, thereby control fluorescent tube quantity control intensity of illumination can satisfy the needs of photosynthetic bacterium luminous energy in every transparent socket.
Packed access opening 10, visor 7 are set on tank body 3 tops, advance steam and water inlet rinse mouth 13, opening for feed 14 and respiratory organ interface 1, whipping appts 8 and discharge port 9 are installed in the inside of tank body 3.Be provided with inlet mouth 2 on tank body 3 tops, feed suitable purify air or carbonic acid gas to the aperture on jar bottom annular pipe extrudes, can provide condition for the aerobic of photosynthetic bacterium or anaerobism demand by inlet pipe 2.Tank body top is provided with Packed access opening 10 and respiratory organ 1 simultaneously, and for maintenance, cleaning and sterilization are provided convenience, respiratory organ can stop a jar outer air to immerse, for the condition that provides of cultivating is provided bacterium.
In the culturing process, add the bottom that all can be deposited on tank body 3 of mixing of nutriment, whipping appts 8 is installed in the inside of tank body 3 with the part thalline, can head it off.At tank body 3 tops, sidewall a plurality of visors 7 are installed, can multi-facetedly observe the situation of jar interior bacterium and nutrient solution.
On tank body 2 sidewalls, temperature probe 11 is installed, simultaneously on the bottom of tank body 2 and sidewall, interlayer 5 is housed, cold and hot matchmaker's import 12 and outlet 6 also are installed on chuck.Adopting water is that cold and hot matchmaker controls hot and cold water-suppling electromagnetic valve switch by temperature probe 11 by temperature controller, thus the temperature of nutrient solution in the controlling tank.Jar interior culture-liquid temp remains in the best microbial culture desired temperature by the automatic control of temperature controller in the training period.

Claims (6)

1. photosynthetic bacterium culture tank, it is characterized in that, described tank body is an interlayer, cold and hot matchmaker's import and outlet are housed on interlayer, on tank body, be provided with the temperature regulation control device, the illuminating lamp tube that 1-10 wavelength is 450~550 μ m is distributed on tank inner chamber, is provided with the inlet pipe that extends to the tank inner chamber bottom from the tank body top on tank body, in tank base whipping appts is housed.
2. photosynthetic bacterium culture tank according to claim 1 is characterized in that, described fluorescent tube is 6 fluorescent lamps or incandescent light, and 6 fluorescent tubes are contained in 6 clear lamp sockets,, axial array circumferentially even along tank body.
3. photosynthetic bacterium culture tank according to claim 1 is characterized in that, the respiratory organ interface that stops jar outer air to enter is equipped with on described tank body top.
4. photosynthetic bacterium culture tank according to claim 1 is characterized in that, the volume of described tank body is 1000~5000 liters.
5. photosynthetic bacterium culture tank according to claim 1 is characterized in that, is equipped with 1-4 on the described tank wall and observes visor.
6. photosynthetic bacterium culture tank according to claim 1 is characterized in that described tank body top or sidepiece are provided with access hole.
CN 201029092045 2010-03-01 2010-03-01 Photosynthetic bacterium cultivating pot Expired - Fee Related CN201605272U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 201029092045 CN201605272U (en) 2010-03-01 2010-03-01 Photosynthetic bacterium cultivating pot

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 201029092045 CN201605272U (en) 2010-03-01 2010-03-01 Photosynthetic bacterium cultivating pot

Publications (1)

Publication Number Publication Date
CN201605272U true CN201605272U (en) 2010-10-13

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Country Status (1)

Country Link
CN (1) CN201605272U (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102973961A (en) * 2012-11-22 2013-03-20 华侨大学 Ozone sterilization method for photobioreactor for culturing photosynthetic bacteria in large scale
CN102994424A (en) * 2012-11-22 2013-03-27 华侨大学 Method for efficiently and continuously collecting super-concentrated photosynthetic bacteria thalli
CN109400241A (en) * 2019-01-09 2019-03-01 苏州寰宝农业科技有限公司 A kind of cultivation bucket and its use the method for preparing organic selenium fertilizer

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102973961A (en) * 2012-11-22 2013-03-20 华侨大学 Ozone sterilization method for photobioreactor for culturing photosynthetic bacteria in large scale
CN102994424A (en) * 2012-11-22 2013-03-27 华侨大学 Method for efficiently and continuously collecting super-concentrated photosynthetic bacteria thalli
CN102994424B (en) * 2012-11-22 2015-08-05 华侨大学 A kind of high-efficiency and continuous collects the method for hyperconcentration photosynthetic bacterial thallus
CN109400241A (en) * 2019-01-09 2019-03-01 苏州寰宝农业科技有限公司 A kind of cultivation bucket and its use the method for preparing organic selenium fertilizer

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C14 Grant of patent or utility model
C41 Transfer of patent application or patent right or utility model
ASS Succession or assignment of patent right

Owner name: HUNAN HUINONG TECHNOLOGY CO., LTD.

Free format text: FORMER OWNER: HUNAN BENZHI BIOTECHNOLOGY CO., LTD.

Effective date: 20140916

TR01 Transfer of patent right

Effective date of registration: 20140916

Address after: 410005 No. 80 middle Huang Xing Road, Furong district, Hunan, Changsha

Patentee after: HUNAN HUINONG TECHNOLOGY CO., LTD.

Address before: 410005 No. 80 middle Huang Xing Road, Hunan, Changsha

Patentee before: Hunan Benzhi Biotechnology Co., Ltd.

CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20101013

Termination date: 20190301

CF01 Termination of patent right due to non-payment of annual fee