CN107488581A - A kind of photosynthetic bacteria production equipment - Google Patents
A kind of photosynthetic bacteria production equipment Download PDFInfo
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- CN107488581A CN107488581A CN201710960303.1A CN201710960303A CN107488581A CN 107488581 A CN107488581 A CN 107488581A CN 201710960303 A CN201710960303 A CN 201710960303A CN 107488581 A CN107488581 A CN 107488581A
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- photosynthetic bacteria
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/20—Material Coatings
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/22—Transparent or translucent parts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M31/00—Means for providing, directing, scattering or concentrating light
- C12M31/10—Means for providing, directing, scattering or concentrating light by light emitting elements located inside the reactor, e.g. LED or OLED
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
Abstract
A kind of photosynthetic bacteria production equipment of disclosure of the invention.Outer cylinder body is made by using the safety glass of bonding, and transparent built-in staving, it is easy to observe the growing state of photosynthetic bacteria, and volume can adjust as requested, also include ultraviolet disinfection bactericidal unit, reach the effect of thorough disinfection, improved the production environment of photosynthetic bacteria, improve the product quality of aquaculture photosynthetic bacteria.
Description
Technical field
The invention belongs to the production equipment of photosynthetic bacteria, more particularly to a kind of special photosynthetic bacteria life of the water transfer of adjustable volume
Produce equipment.
Background technology
Photosynthetic bacteria（Photosynthetic bacteria, abbreviation PSB）It is a group energy in anaerobism illumination or aerobic dark
Under the conditions of using organic matter make oxygen donator and carbon source, do not put the photosynthetic bacterium of oxygen, be the tool occurred earliest on the earth
Have the prokaryotes of original luminous energy synthetic system, be distributed widely in the paddy field of nature, lakes and marhshes, rivers, ocean, activated sludge and
It is more common in the high waters of spoilage organism concentration in soil.According to relevant classification, photosynthetic bacteria can be divided into 6 classes
Group, 27 category.The photosynthetic Rhodospirillales of non-oxygen-production is divided into purple bacteria（purple bacteria）, green bacterium
（Greenbacteria）With day Photorhabdus（Heliobacteria）, bacillus rubidus category（Erybrobacter）.It is wherein purple thin
Include Rhodospirillaceae in bacterium（Rhodolspirillaceae）, Chromatiaceae（Chromatiaceae）, outer sulphur Rhodospirillaceae
（Eceothiorhodospiraceae）, include 16 49 kinds of category.Significant Rhodospirillaceae includes red spirillum wherein in production
Category, Rhodopseudomonas and Rhodomicrobium.PSB is gramnegative bacterium, its form of diverse, have ball-type, avette, rod,
Arc, spiral shape, annular, semi-circular silk shape；There are unicellular and many cells；Also can change with condition of culture and growth phase,
Most of single presence.
Photosynthetic bacteria nutritional ingredient very abundant, PSB thalline contain 65.45% protein, and 7.18% fat, 2.78% is thick
Fiber, 20.31% soluble sugar, 4.28% ash content.Its amino acid content enriches after PSB proteolysis.PSB thalline contain abundant
B family vitamin, wherein VB12 contents are 21 μ g/g, are 200 times of yeast.PSB thalline are also containing Co-Q10, class Hu
Radish element, the content of wherein Co-Q10 is respectively the 13 of the content of yeast, the leaf of spinach and Western corn Co-Q10,94 and 82
Times.PSB thalline photosynthetic pigments are made up of bacteriochlorophyll (Bch1) and carotenoid (Carotenoids), it has now been found that
Bacteriochlorophyll has a, b, c, d, e5 kind, every kind of to have fixed light absorption wavelength;It has been found that carotenoids are known as kind more than 80,
Including spirilloxanthin, rhodopin, spheroidene, lycopene, lutein etc..Carotenoids in PSB contained by different bacterial strains
The species of element is different, and research finds that the class Hu Luosu extracted from Rhodobacter sphaeroidcs is mainly beta carotene, and from
The mainly lycopene extracted in folder film red pseudomonas.Chlorophylls and Carotenoids increase to the healthy growth of aquaculture organism
There is very big benefit to the resistance of disease by force.CoQ4 is the physiological activator for having significant relationship with vital movement, the above
Feature determines that PSB can be as livestock and poultry, the feed of fishes and shrimps.Aquaculture market to the demand gaps of P S B live body strains very
Greatly, in order to meet the needs of aquaculture is to PSB live body strains, PSB research how is made to walk out laboratory, and as soon as possible should
With with produce reality, it is important to realize large-scale production.
At present, production photosynthetic bacteria uses bioreactor more, and cost is higher, and each installed capacity is limited,
The growing state of Real Time Observation photosynthetic bacteria is unable to, if to realize plant layout continuous production, cost of investment is higher.Therefore,
Develop a kind of photosynthetic bacteria large-scale production equipment, solve photosynthetic bacteria production equipment capacity in the prior art can not adjust, into
This cost is high, can not observe the technical problems such as photosynthetic bacterium growth situation to realize efficient, inexpensive scale photosynthetic bacteria
Production, it is the effective way for the sustainable health development for promoting green culture fishery, to promoting China's aquaculture industry
High-efficient development is significant.
The content of the invention
In order to solve the above-mentioned technical problem, the invention provides a kind of photosynthetic bacteria production equipment, the production equipment tool
Have：Cylinder body, ultraviolet disinfection bactericidal unit, lighting device and auxiliary stand, the ultraviolet disinfection bactericidal unit are used for ultraviolet disinfection
Sterilizing, the lighting device are used to provide illumination, and the agitating device is used to stir nutrient solution, it is characterised in that also has and stirs
Device is mixed, the agitating device is used to stir nutrient solution.
As the another optimal technical scheme of the present invention, the cylinder body is made up of outer cylinder body and built-in staving.
As the another optimal technical scheme of the present invention, the outer cylinder body is formed by safety glass bonding, is easy to photosynthetic thin
Bacterium receives illumination, and volume can be adjusted according to production requirement.
As the another optimal technical scheme of the present invention, the built-in staving is transparent circular, is easy to photosynthetic bacteria receiving
Illumination, and volume can be adjusted according to production requirement.
As the another optimal technical scheme of the present invention, left between the outer cylinder body and the built-in staving and ultraviolet disappear is installed
The space of malicious bactericidal unit and lighting device.
As the another optimal technical scheme of the present invention, it is provided between the outer cylinder body and the built-in staving to described interior
Put the water circulation system that staving is heated and is incubated.
As the another optimal technical scheme of the present invention, the ultraviolet disinfection bactericidal unit is placed as diagonal.
As the another optimal technical scheme of the present invention, the lighting device is close to for that can wind soft water proofing property LED
The built-in staving spiral wound is on bucket outer wall.
As the another optimal technical scheme of the present invention, the agitating device, which is furnished with, scrapes brushing piece.
Step and parameter in above-mentioned production technology be inventor on the basis of creative work is paid, carried out
Optimum choice, the present invention is easy to operation, cost of investment is low, volume of equipment is adjustable, bacterial contamination rate is low in production process.
The finished product photosynthetic bacteria of production, inoculum density is high, and miscellaneous bacteria rate is low, and quality is good.
Present invention also offers a kind of obtained aquaculture photosynthetic bacteria of photosynthetic bacteria production equipment using the present invention,
Wherein, the bacterial contamination rate of the photosynthetic bacteria is low, and product quality is improved.
The above-mentioned technical proposal of the present invention at least has following technique effect：
（1）Using the cylinder body of bonding, the adjustable volume of production equipment is realized, the versatility of production equipment is ensure that, beneficial to reality
The serialization and scale of existing photosynthetic bacteria production.
（2）Two-layer barrel body is transparent material, by installing uviol lamp among two-layer barrel body, reaches the effect of sterilization
Fruit；Soft Water proof LED lamp tube is installed, ensures that photosynthetic bacterium growth has sufficient illumination, is advantageous to improve the production of photosynthetic bacteria
Efficiency.
（3）Between two-layer barrel body and the space of abundance is left, is heated using recirculated water, ensures that photosynthetic bacteria is in suitably
At a temperature of growth and breeding, realize the optimization of photosynthetic bacterium growth environment.
（4）Because the phototaxis of photosynthetic bacteria, having part photosynthetic bacteria can be attached on built-in staving, if not in time
Cleaning, the transmitance of illumination can be influenceed, agitating device of the invention forms for voluntarily research and development transformation, and is furnished with special wiper
Piece, it is easy to clean built-in staving.
（5）It is easy to operation, cost of investment is low, volume of equipment is adjustable, bacterial contamination rate is low in production process.Production
Finished product photosynthetic bacteria, inoculum density is high, and miscellaneous bacteria rate is low, and quality is good.
Brief description of the drawings
Fig. 1 is the production equipment sketch of the present invention.
Embodiment
Embodiments of the present invention are described in further detail with reference to the accompanying drawings and examples.Following examples are used for
Illustrate the present invention, but cannot be used for limiting the scope of the present invention.
" one embodiment " or " embodiment " referred to herein refers to may be included at least one implementation of the present invention
Special characteristic, structure or characteristic." in one embodiment " that different places occur in this manual not refers both to same
Individual embodiment, nor the single or selective embodiment mutually exclusive with other embodiment.
As shown in figure 1, the production equipment of the present invention has：Charging aperture 1 and discharging opening 5, also with transparent outer cylinder body and
Built-in staving, leaves enough spaces between outer cylinder body and built-in staving, and the space sets the He of low-temperature energy-saving fluorescent lamp 2 enough
Uviol lamp 3, wherein, the uviol lamp is placed as diagonal, is made to receive abundant ultraviolet irradiation inside built-in entire body, is reached sterilization
Effect；The fluorescent lamp 2 is close to built-in staving spiral wound in entire body, met for that can wind soft water proofing property LED
The illumination requirement of photosynthetic bacterium growth；Circulation is additionally provided with the space, water circulation heating is carried out to built-in staving
Insulation, meet the temperature requirement of photosynthetic bacterium growth；The outer cylinder body is formed by safety glass bonding, is easy to photosynthetic bacteria receiving
Illumination, and volume can be adjusted according to production requirement；The volume of the built-in staving can also be carried out according to production requirement
Adjustment；The production equipment of the present invention also has agitating device, and the agitating device forms for voluntarily research and development transformation, and equipped with special
Scrape brushing piece.
Embodiment 1
A kind of production equipment of the special photosynthetic bacteria product of water transfer, the equipment volume about 1m3, including（1）Outer cylinder body is circle, directly
Through being highly 1.2m for 1.2m；Cylinder body is formed by safety glass bonding, safety glass thickness 10mm；（2）Built-in staving is circle
Shape, a diameter of 1.08m, it is highly 1.2m；6cm distance is left among outer cylinder body and built-in staving；（3）Cylinder cap passes through four cards
Hoop is connected with cylinder body, and cylinder cap horizontal diameter is 1.2m, and cylinder cap is provided with charging aperture；（4）Cylinder/bucket bottom materials are safety glass,
It is connected by glass cement with outer cylinder body and built-in staving, cylinder/bucket bottom connects a liquid valve door, and whole cylinder body is placed on height and is
In 50cm stainless steel shelf；（5）Uviol lamp, power 30W, length 105cm, 4, in being diagonally placed in outer cylinder body and built-in bucket
Among body, fixed with glass cement；（6）Soft Water proof LED lamp tube, environmentally friendly gum cover, power 3/6W, length 8m, pure white, 1, spiral
Shape is wrapped on built-in staving, and built-in bucket outer wall is fixed on glass cement；（7）Agitating device, acc power 2.22KW is stirred, Gu
Body upper cylinder cover center, stirring rod vertical length 90cm, is laterally divided into two layers, and length is respectively 50cm, 80cm, in 30 °；Two groups special
System scrapes brushing piece, is fixed in stirring rod.
High temperature season, water circulation heating need not be carried out during production, directly by pure water, culture medium from charging aperture put into built in bucket
It is interior, open and stir, after stirring evenly, open uviol lamp, after the 2h that sterilizes, close uviol lamp and put into photosynthetic bacteria original in factory formula ratio
Kind, stirring is opened, is stirred evenly, LED lamp tube is opened, is cultivated, culture carries out filling after about one week；Low temperature season, will during production
Open water circulation and carry out heating and thermal insulation.
After production equipment using the present invention, at least following points benefit：（1）Using the cylinder body of bonding, life is realized
The adjustable volume of equipment is produced, ensure that the versatility of production equipment, beneficial to the serialization and scale for realizing photosynthetic bacteria production.
（2）Two-layer barrel body is transparent material, by installing uviol lamp among two-layer barrel body, reaches the effect of sterilization
Fruit；Soft Water proof LED lamp tube is installed, ensures that photosynthetic bacterium growth has sufficient illumination, is advantageous to improve the production of photosynthetic bacteria
Efficiency.
（3）Between two-layer barrel body and the space of abundance is left, is heated using recirculated water, ensures that photosynthetic bacteria is in suitably
At a temperature of growth and breeding, realize the optimization of photosynthetic bacterium growth environment.
（4）Because the phototaxis of photosynthetic bacteria, having part photosynthetic bacteria can be attached on built-in staving, if not in time
Cleaning, the transmitance of illumination can be influenceed, agitating device of the invention forms for voluntarily research and development transformation, and is furnished with special wiper
Piece, it is easy to clean built-in staving.
（5）It is easy to operation, cost of investment is low, volume of equipment is adjustable, bacterial contamination rate is low in production process.Production
Finished product photosynthetic bacteria, inoculum density is high, and miscellaneous bacteria rate is low, and quality is good.
Although embodiment of the present invention is disclosed as above, it is not restricted in specification and embodiment listed
With it can be applied to the field of the invention completely, for those skilled in the art, be easily achieved in addition
Modification, therefore under the universal limited without departing substantially from claim and equivalency range, the present invention is not limited to specifically
Details.
Claims (10)
1. a kind of photosynthetic bacteria production equipment, the production equipment include cylinder body, ultraviolet disinfection bactericidal unit, lighting device and
Auxiliary stand, the ultraviolet disinfection bactericidal unit sterilize for ultraviolet disinfection, and the lighting device is used to provide illumination, its feature
It is, also is used to stir nutrient solution with agitating device, the agitating device.
2. a kind of photosynthetic bacteria production equipment as claimed in claim 1, it is characterised in that the cylinder body is by outer cylinder body and interior
Put staving composition.
3. a kind of photosynthetic bacteria production equipment as claimed in claim 2, it is characterised in that the outer cylinder body is glued by safety glass
Connect and form, be easy to photosynthetic bacteria and receive illumination, and volume can be adjusted according to production requirement.
4. a kind of photosynthetic bacteria production equipment as claimed in claim 2 or claim 3, it is characterised in that the built-in staving is transparent
Circle, it is easy to photosynthetic bacteria and receives illumination, and volume can be adjusted according to production requirement.
A kind of 5. photosynthetic bacteria production equipment as claimed in claim 2 or claim 3, it is characterised in that the outer cylinder body with it is described interior
Put the space that installation ultraviolet disinfection bactericidal unit and lighting device are left between staving.
A kind of 6. photosynthetic bacteria production equipment as claimed in claim 2 or claim 3, it is characterised in that the outer cylinder body with it is described interior
Put and the water circulation system that the built-in staving is heated and is incubated is installed between staving.
7. a kind of photosynthetic bacteria production equipment as claimed in claim 1 or 2, it is characterised in that it is characterized in that described ultraviolet
Disinfector is placed as diagonal.
8. a kind of photosynthetic bacteria production equipment as claimed in claim 2 or claim 3, it is characterised in that the lighting device is to twine
Around soft water proofing property LED, it is close to the built-in staving spiral wound on cylinder.
9. a kind of photosynthetic bacteria production equipment as claimed in claim 1 or 2, it is characterised in that the agitating device is furnished with wiper
Piece.
10. a kind of aquaculture photosynthetic bacteria, it is characterised in that using photosynthetic thin as claimed in any one of claims 1-9 wherein
Bacterium production equipment is prepared.
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| CN201710960303.1A CN107488581A (en) | 2017-10-16 | 2017-10-16 | A kind of photosynthetic bacteria production equipment |
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| CN201710960303.1A CN107488581A (en) | 2017-10-16 | 2017-10-16 | A kind of photosynthetic bacteria production equipment |
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| JPH11243946A (en) * | 1998-02-27 | 1999-09-14 | Matsumoto Biseibutsu Kenkyusho:Kk | Mass-culture of photosynthetic bacteria |
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| CN201915083U (en) * | 2010-09-13 | 2011-08-03 | 江苏邦德生物科技发展有限公司 | Efficient culturing device for photosynthetic bacteria |
| CN202246648U (en) * | 2011-09-23 | 2012-05-30 | 农业部规划设计研究院 | Rotary sealing light distribution device suitable for cylindrical micro algae reactor |
| CN103756869A (en) * | 2013-12-31 | 2014-04-30 | 江苏元洲生物工程有限公司 | Adherent cell removing device of illuminating fermentation tank |
| CN107142233A (en) * | 2017-07-11 | 2017-09-08 | 江苏苏港和顺生物科技有限公司 | A kind of aquaculture photosynthetic bacteria and its production technology |
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2017
- 2017-10-16 CN CN201710960303.1A patent/CN107488581A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH11243946A (en) * | 1998-02-27 | 1999-09-14 | Matsumoto Biseibutsu Kenkyusho:Kk | Mass-culture of photosynthetic bacteria |
| CN201605272U (en) * | 2010-03-01 | 2010-10-13 | 湖南本质生物科技有限公司 | Photosynthetic bacterium cultivating pot |
| CN201915083U (en) * | 2010-09-13 | 2011-08-03 | 江苏邦德生物科技发展有限公司 | Efficient culturing device for photosynthetic bacteria |
| CN202246648U (en) * | 2011-09-23 | 2012-05-30 | 农业部规划设计研究院 | Rotary sealing light distribution device suitable for cylindrical micro algae reactor |
| CN103756869A (en) * | 2013-12-31 | 2014-04-30 | 江苏元洲生物工程有限公司 | Adherent cell removing device of illuminating fermentation tank |
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Application publication date: 20171219 |