CN107488581A - A kind of photosynthetic bacteria production equipment - Google Patents
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Abstract
Description
技术领域technical field
本发明属于光合细菌的生产设备，特别涉及一种容积可调的调水专用光合细菌生产设备。The invention belongs to the production equipment of photosynthetic bacteria, in particular to a special photosynthetic bacteria production equipment for water adjustment with adjustable volume.
背景技术Background technique
光合细菌（photosynthetic bacteria,简称PSB）是一群能在厌氧光照或好氧黑暗条件下利用有机物作供氧体兼碳源，进行不放氧光合作用的细菌，是地球上最早出现的具有原始光能合成体系的原核生物，广泛分布于自然界的水田、湖沼、江河、海洋、活性污泥和土壤中，在腐败有机物浓度高的水域中更为常见。根据相关分类，光合细菌可分为6 个类群，27 个属。不产氧光合作用的红螺菌目分为紫细菌（purple bacteria）、绿细菌（Greenbacteria）和日光杆菌属（Heliobacteria）、红色杆菌属（Erybrobacter）。其中紫细菌中包含有红螺菌科（Rhodolspirillaceae）、着色菌科（Chromatiaceae）、外硫红螺菌科（Eceothiorhodospiraceae），包含16属49种。其中在生产上有意义的红螺菌科包括红螺菌属、红假单胞菌属和红微菌属。PSB 均为革兰氏阴性细菌，其形态多样，有球型、卵形、杆形、弧形、螺旋形、环形、半环形丝形；有单细胞和多细胞；也可随培养条件和生长阶段而改变，大部分单个存在。Photosynthetic bacteria (photosynthetic bacteria, referred to as PSB) is a group of bacteria that can use organic matter as oxygen donor and carbon source under anaerobic light or aerobic dark conditions to carry out oxygen-free photosynthesis. Prokaryotes capable of synthesizing the system are widely distributed in paddy fields, lakes, rivers, oceans, activated sludge and soil in nature, and are more common in waters with high concentrations of decaying organic matter. According to related classification, photosynthetic bacteria can be divided into 6 groups and 27 genera. Rhodospirillales that do not produce oxygen and photosynthesis are divided into purple bacteria, green bacteria, Heliobacteria, and Erybrobacter. Among them, purple bacteria include Rhodolspirillaceae, Chromatiaceae, and Eceothiorhodospiraceae, including 16 genera and 49 species. Among them, the Rhodospirillaceae which are meaningful in production include Rhodospirillum, Rhodopseudomonas and Rhodomicrobacterium. PSB are Gram-negative bacteria with various shapes, including spherical, oval, rod-shaped, arc-shaped, spiral, circular, and semi-circular filaments; unicellular and multicellular; they can also vary with culture conditions and growth Phases vary, most exist singly.
光合细菌营养成分十分丰富，PSB菌体含有65.45%蛋白质, 7.18%脂肪, 2.78%粗纤维, 20.31%可溶性糖, 4.28%灰分。PSB蛋白水解后其氨基酸含量丰富。PSB菌体含有丰富的B族维生素, 其中VB12含量为21 μg/g, 是酵母的200倍。PSB菌体还含有辅酶Q10 、类胡萝卜素, 其中辅酶Q10的含量分别为酵母、菠菜叶和玉米幼芽辅酶Q10的含量的13、94和82倍。PSB菌体光合色素由细菌叶绿素(Bch1)和类胡萝卜素(Carotenoids)组成, 现已发现的细菌叶绿素有a、b、c、d、e5种, 每种都有固定的光吸收波长;已发现类胡萝卜素有80多种,其中包括螺菌黄素、玫红品、球形烯、番茄红素、叶黄素等。PSB中不同的菌株所含的类胡萝卜素的种类不同, 研究发现从浑球红假单胞菌中提取的类胡萝素主要是β -胡萝卜素, 而从夹膜红假单胞菌中提取的主要是番茄红素。叶绿素和类胡萝卜素对养殖生物的健康生长，增强对疾病的抵抗力有很大的益处。辅酶Q4 是与生命活动有重大关系的生理活性物质，以上特点决定了PSB 可做为畜禽、鱼虾的饲料。水产养殖市场对P S B 活体菌种的需求缺口很大，为了满足水产养殖对PSB 活体菌种的需求，如何使PSB的研究走出实验室，并尽快地应用与生产实际，关键是实现规模生产。Photosynthetic bacteria are very rich in nutrients. PSB cells contain 65.45% protein, 7.18% fat, 2.78% crude fiber, 20.31% soluble sugar, and 4.28% ash. After PSB proteolysis, its amino acid content is abundant. PSB cells are rich in B vitamins, among which the content of VB12 is 21 μg/g, which is 200 times that of yeast. PSB cells also contain coenzyme Q10 and carotenoids, and the content of coenzyme Q10 is 13, 94 and 82 times that of yeast, spinach leaves and corn sprouts, respectively. The photosynthetic pigments of PSB bacteria are composed of bacteriochlorophyll (Bch1) and carotenoids (Carotenoids). There are 5 species of bacteriochlorophyll found, a, b, c, d, and e, each of which has a fixed light absorption wavelength; it has been found that There are more than 80 kinds of carotenoids, including spiroxanthin, rosepin, globene, lycopene, lutein and so on. Different strains of PSB contain different types of carotenoids. Studies have found that the carotenoids extracted from Rhodopseudomonas sphaeroides are mainly β-carotene, while the carotenoids extracted from Rhodopseudomonas capsularis Mainly lycopene. Chlorophyll and carotenoids are of great benefit to the healthy growth of farmed organisms and to enhance their resistance to disease. Coenzyme Q4 is a physiologically active substance that has a significant relationship with life activities. The above characteristics determine that PSB can be used as feed for livestock, poultry, fish and shrimp. The aquaculture market has a large demand gap for PSB live strains. In order to meet the demand for PSB live strains in aquaculture, the key is to realize the large-scale production of PSB research out of the laboratory and apply it to actual production as soon as possible.
目前，生产光合细菌多采用光生物反应器，成本造价较高，且每个装置容量有限，不能实时观察光合细菌的生长情况，如果要实现工厂规模连续生产，投资成本较高。因此，开发一种光合细菌规模化生产设备，解决现有技术中光合细菌生产设备容量不可调整、成本造价高、不能观察光合细菌生长情况等技术问题以实现高效、低成本的规模化光合细菌生产，是促进绿色水产养殖业的健康可持续发展的有效途径，对推动我国水产养殖产业的高效发展具有重要意义。At present, photobioreactors are mostly used for the production of photosynthetic bacteria, and the cost is high, and the capacity of each device is limited, and the growth of photosynthetic bacteria cannot be observed in real time. If continuous production on a factory scale is to be achieved, the investment cost is relatively high. Therefore, a large-scale production equipment for photosynthetic bacteria is developed to solve technical problems such as non-adjustable capacity of photosynthetic bacteria production equipment in the prior art, high cost, and inability to observe the growth of photosynthetic bacteria to achieve high-efficiency and low-cost large-scale production of photosynthetic bacteria , is an effective way to promote the healthy and sustainable development of the green aquaculture industry, and is of great significance to promote the efficient development of my country's aquaculture industry.
发明内容Contents of the invention
为了解决上述技术问题，本发明提供了一种光合细菌生产设备，所述生产设备具有：缸体、紫外消毒灭菌装置、照明装置和辅助支架，所述紫外消毒灭菌装置用于紫外消毒灭菌，所述照明装置用于提供光照，所述搅拌装置用于搅拌营养液，其特征在于，还具有搅拌装置，所述搅拌装置用于搅拌营养液。In order to solve the above technical problems, the present invention provides a photosynthetic bacteria production equipment, the production equipment has: cylinder body, ultraviolet disinfection and sterilization device, lighting device and auxiliary bracket, the ultraviolet disinfection and sterilization device is used for ultraviolet disinfection and sterilization bacteria, the lighting device is used to provide light, the stirring device is used to stir the nutrient solution, and it is characterized in that it also has a stirring device, and the stirring device is used to stir the nutrient solution.
作为本发明的又一优选技术方案，所述缸体是由外缸体和内置桶体组成的。As another preferred technical solution of the present invention, the cylinder body is composed of an outer cylinder body and an inner barrel body.
作为本发明的又一优选技术方案，所述外缸体由钢化玻璃粘接而成，易于光合细菌接受光照，且体积可以根据生产要求进行调整。As another preferred technical solution of the present invention, the outer cylinder body is made of tempered glass, which is easy for photosynthetic bacteria to receive light, and the volume can be adjusted according to production requirements.
作为本发明的又一优选技术方案，所述内置桶体为透明圆形，易于光合细菌接受光照，且体积可以根据生产要求进行调整。As another preferred technical solution of the present invention, the built-in barrel is transparent and circular, which is easy for photosynthetic bacteria to receive light, and the volume can be adjusted according to production requirements.
作为本发明的又一优选技术方案，所述外缸体与所述内置桶体间留有安装紫外消毒灭菌装置和照明装置的空间。As another preferred technical solution of the present invention, there is a space for installing ultraviolet disinfection and sterilization devices and lighting devices between the outer cylinder body and the inner barrel body.
作为本发明的又一优选技术方案，所述外缸体与所述内置桶体间安装有对所述内置桶体进行加热和保温的水循环系统。As another preferred technical solution of the present invention, a water circulation system for heating and keeping warm the built-in barrel is installed between the outer cylinder and the built-in barrel.
作为本发明的又一优选技术方案，所述紫外消毒灭菌装置按对角放置。As yet another preferred technical solution of the present invention, the ultraviolet disinfection and sterilization devices are placed diagonally.
作为本发明的又一优选技术方案，所述照明装置为可缠绕软质防水性LED灯，紧贴所述内置桶体螺旋状缠绕在桶体外壁上。As yet another preferred technical solution of the present invention, the illuminating device is a windable soft waterproof LED lamp, which is tightly attached to the built-in barrel body and wound spirally on the outer wall of the barrel.
作为本发明的又一优选技术方案，所述搅拌装置配有刮刷片。As another preferred technical solution of the present invention, the stirring device is equipped with a scraper blade.
上述生产工艺中的步骤和参数均是发明人在付出创造性劳动的基础上，所进行的优化选择，本发明易于工人操作、投资成本低、设备容积可调整、生产过程中杂菌污染率低。生产的成品光合细菌，菌种密度高，杂菌率低，质量好。The steps and parameters in the above-mentioned production process are optimized and selected by the inventors on the basis of creative work. The invention is easy for workers to operate, low in investment costs, adjustable in equipment volume, and low in bacteria contamination rate in the production process. The finished photosynthetic bacteria produced have high strain density, low miscellaneous bacteria rate and good quality.
本发明还提供了一种利用本发明的光合细菌生产设备得到的水产养殖光合细菌，其中，所述光合细菌的杂菌污染率低，产品质量得到了提高。The present invention also provides an aquaculture photosynthetic bacterium obtained by using the photosynthetic bacterium production equipment of the present invention, wherein the photosynthetic bacterium has a low miscellaneous bacteria pollution rate and the product quality is improved.
本发明的上述技术方案至少具有以下技术效果：The technical solution of the present invention has at least the following technical effects:
（1）采用粘接的缸体，实现了生产设备的容积可调，保证了生产设备的通用性，利于实现光合细菌生产的连续化和规模化。(1) The use of bonded cylinders realizes the adjustable volume of the production equipment, ensures the versatility of the production equipment, and facilitates the continuous and large-scale production of photosynthetic bacteria.
（2）双层桶体为透明材质，通过在双层桶体中间安装紫外灯，达到消毒灭菌的效果；安装软防水LED灯管，保证光合细菌生长具有充足的光照，有利于提高光合细菌的生产效率。(2) The double-layer barrel is made of transparent material. By installing an ultraviolet lamp in the middle of the double-layer barrel, it can achieve the effect of disinfection and sterilization; install a soft waterproof LED tube to ensure that the growth of photosynthetic bacteria has sufficient light, which is conducive to improving the growth of photosynthetic bacteria. production efficiency.
（3）双层桶体之间并且留有充足的空间，采用循环水加温，保证光合细菌处在适宜的温度下生长繁殖，实现了光合细菌生长环境的优化。(3) Sufficient space is left between the double-layer barrels, and circulating water is used for heating to ensure the growth and reproduction of photosynthetic bacteria at a suitable temperature, which realizes the optimization of the growth environment of photosynthetic bacteria.
（4）因为光合细菌的趋光性，会有部分光合细菌会附着在内置桶体上，如果不及时清理，会影响光照的透过率，本发明的搅拌装置为自行研发改造而成，且配有特制的刮刷片，便于对内置桶体进行清洗。(4) Due to the phototaxis of photosynthetic bacteria, some photosynthetic bacteria will adhere to the built-in barrel. If not cleaned in time, it will affect the light transmittance. The mixing device of the present invention is developed and modified by itself, and is equipped with The special scraper is convenient for cleaning the built-in barrel.
（5）易于工人操作、投资成本低、设备容积可调整、生产过程中杂菌污染率低。生产的成品光合细菌，菌种密度高，杂菌率低，质量好。(5) It is easy for workers to operate, low investment cost, adjustable equipment volume, and low bacteria contamination rate in the production process. The finished photosynthetic bacteria produced have high strain density, low miscellaneous bacteria rate and good quality.
附图说明Description of drawings
图1为本发明的生产设备简图。Fig. 1 is a schematic diagram of the production equipment of the present invention.
具体实施方式detailed description
下面结合附图和实施例对本发明的实施方式作进一步详细描述。以下实施例用于说明本发明，但不能用于限制本发明的范围。Embodiments of the present invention will be further described in detail below in conjunction with the accompanying drawings and examples. The following examples are used to illustrate the present invention, but not to limit the scope of the present invention.
此处所称的“一个实施例”或“实施例”是指可包含于本发明至少一个实现方式中的特定特征、结构或特性。在本说明书中不同地方出现的“在一个实施例中”并非均指同一个实施例，也不是单独的或选择性的与其他实施例互相排斥的实施例。Reference herein to "one embodiment" or "an embodiment" refers to a particular feature, structure or characteristic that can be included in at least one implementation of the present invention. "In one embodiment" appearing in different places in this specification does not all refer to the same embodiment, nor is it a separate or selective embodiment that is mutually exclusive with other embodiments.
如图1所示，本发明的生产设备具有：进料口1和出料口5，还具有透明的外缸体和内置桶体，在外缸体和内置桶体之间留有足够的空间，该空间足够设置低温节能日光灯2和紫外灯3，其中，所述紫外灯按对角放置，使内置通体内部接受充分紫外照射，达到消毒灭菌的效果；所述日光灯2为可缠绕软质防水性LED灯，紧贴内置桶体螺旋状缠绕在通体上，满足光合细菌生长的光照要求；所述空间内还设置有循环水系统，对内置桶体进行水循环加热保温，满足光合细菌生长的温度要求；所述外缸体由钢化玻璃粘接而成，易于光合细菌接受光照，且体积可以根据生产要求进行调整；所述内置桶体的体积也可以根据生产要求进行调整；本发明的生产设备还具有搅拌装置，所述搅拌装置为自行研发改造而成，且配有特制的刮刷片。As shown in Figure 1, the production equipment of the present invention has: feed inlet 1 and discharge outlet 5, also have transparent outer cylinder body and built-in barrel, leave enough space between outer cylinder body and built-in barrel, The space is enough to install low-temperature energy-saving fluorescent lamps 2 and ultraviolet lamps 3, wherein the ultraviolet lamps are placed diagonally so that the inside of the built-in whole body can receive sufficient ultraviolet radiation to achieve the effect of disinfection and sterilization; the fluorescent lamp 2 is a windable soft waterproof Permanent LED lights, which are tightly attached to the built-in barrel and spirally wound on the whole body, meet the lighting requirements for the growth of photosynthetic bacteria; the space is also equipped with a circulating water system, which performs water circulation heating and heat preservation on the built-in barrel to meet the growth temperature of photosynthetic bacteria Requirements; the outer cylinder body is made of tempered glass, which is easy for photosynthetic bacteria to accept light, and the volume can be adjusted according to production requirements; the volume of the built-in barrel body can also be adjusted according to production requirements; the production equipment of the present invention It also has a stirring device, which is self-developed and modified, and is equipped with a special scraping blade.
实施例1Example 1
一种调水专用光合细菌产品的生产设备，该设备体积约1m3，包括（1）外缸体为圆形，直经为1.2m，高度为1.2m；缸体由钢化玻璃粘接而成，钢化玻璃厚度10mm；（2）内置桶体为圆形，直径为1.08m，高度为1.2m；外缸体和内置桶体中间留有6cm的距离；（3）缸盖通过四个卡箍与缸体相连接，缸盖水平直径为1.2m，缸盖上设有进料口；（4）缸/桶底材质为钢化玻璃，通过玻璃胶与外缸体和内置桶体相连接，缸/桶底连接一个出液阀门，整个缸体放在高度为50cm的不锈钢架子上；（5）紫外灯，功率30W，长度105cm，4根，呈对角安置在外缸体和内置桶体中间，用玻璃胶固定；（6）软防水LED灯管，环保胶套，功率3/6W，长度8m，纯白色，1根，螺旋状缠绕在内置桶体上，用玻璃胶固定在内置桶体外壁；（7）搅拌装置，搅拌机功率2.22KW，固体上缸盖中心，搅拌棒竖直长度90cm，横向分为两层，长度分别为50cm、80cm，呈30°；两组特制刮刷片，固定在搅拌棒上。A production equipment for photosynthetic bacteria products specially used for water diversion. The volume of the equipment is about 1m 3 , including (1) the outer cylinder body is circular, with a diameter of 1.2m and a height of 1.2m; the cylinder body is made of tempered glass. , tempered glass thickness 10mm; (2) The built-in barrel is circular, with a diameter of 1.08m and a height of 1.2m; there is a distance of 6cm between the outer cylinder and the built-in barrel; (3) The cylinder head passes through four clamps It is connected with the cylinder body, the horizontal diameter of the cylinder head is 1.2m, and there is a material inlet on the cylinder head; (4) The bottom of the cylinder/barrel is made of tempered glass, which is connected with the outer cylinder body and the built-in cylinder body through glass glue. / The bottom of the barrel is connected to a liquid outlet valve, and the entire cylinder is placed on a stainless steel shelf with a height of 50cm; (5) UV lamps, power 30W, length 105cm, 4 pieces, placed diagonally between the outer cylinder and the built-in barrel, Fix with glass glue; (6) Soft waterproof LED lamp tube, environmental protection rubber sleeve, power 3/6W, length 8m, pure white, 1 piece, spirally wound on the built-in barrel, fixed on the outer wall of the built-in barrel with glass glue (7) Stirring device, the power of the mixer is 2.22KW, the center of the solid upper cylinder head, the vertical length of the stirring rod is 90cm, and the horizontal is divided into two layers, the lengths are 50cm and 80cm respectively, and the angle is 30°; two sets of special scraping blades, fixed on a stir bar.
高温季节，生产时无需进行水循环加热，直接将纯水、培养基从进料口投入内置桶内，开启搅拌，搅匀后，开启紫外灯，灭菌2h后，关闭紫外灯按生产配方比例投入光合细菌原种，开启搅拌，搅匀，开启LED灯管，进行培养，培养约一周后进行灌装；低温季节，生产时要开启水循环进行加热保温。In the high temperature season, there is no need for water circulation heating during production, and the pure water and culture medium are directly put into the built-in barrel from the feeding port, and the stirring is turned on. For the original species of photosynthetic bacteria, turn on the stirring, stir well, turn on the LED light tube, and carry out the cultivation. After about a week of cultivation, it will be filled; in the low temperature season, the water cycle should be turned on for heating and heat preservation during production.
采用本发明的生产设备后，至少有如下几点好处：（1）采用粘接的缸体，实现了生产设备的容积可调，保证了生产设备的通用性，利于实现光合细菌生产的连续化和规模化。After adopting the production equipment of the present invention, there are at least the following advantages: (1) the use of bonded cylinders realizes the adjustable volume of the production equipment, ensures the versatility of the production equipment, and is beneficial to the continuous production of photosynthetic bacteria and scale.
（2）双层桶体为透明材质，通过在双层桶体中间安装紫外灯，达到消毒灭菌的效果；安装软防水LED灯管，保证光合细菌生长具有充足的光照，有利于提高光合细菌的生产效率。(2) The double-layer barrel is made of transparent material. By installing an ultraviolet lamp in the middle of the double-layer barrel, it can achieve the effect of disinfection and sterilization; install a soft waterproof LED tube to ensure that the growth of photosynthetic bacteria has sufficient light, which is conducive to improving the growth of photosynthetic bacteria. production efficiency.
（3）双层桶体之间并且留有充足的空间，采用循环水加温，保证光合细菌处在适宜的温度下生长繁殖，实现了光合细菌生长环境的优化。(3) Sufficient space is left between the double-layer barrels, and circulating water is used for heating to ensure the growth and reproduction of photosynthetic bacteria at a suitable temperature, which realizes the optimization of the growth environment of photosynthetic bacteria.
（4）因为光合细菌的趋光性，会有部分光合细菌会附着在内置桶体上，如果不及时清理，会影响光照的透过率，本发明的搅拌装置为自行研发改造而成，且配有特制的刮刷片，便于对内置桶体进行清洗。(4) Due to the phototaxis of photosynthetic bacteria, some photosynthetic bacteria will adhere to the built-in barrel. If not cleaned in time, it will affect the light transmittance. The mixing device of the present invention is developed and modified by itself, and is equipped with The special scraper is convenient for cleaning the built-in barrel.
（5）易于工人操作、投资成本低、设备容积可调整、生产过程中杂菌污染率低。生产的成品光合细菌，菌种密度高，杂菌率低，质量好。(5) It is easy for workers to operate, low investment cost, adjustable equipment volume, and low bacteria contamination rate in the production process. The finished photosynthetic bacteria produced have high strain density, low miscellaneous bacteria rate and good quality.
尽管本发明的实施方案已公开如上，但其并不仅仅限于说明书和实施方式中所列运用，它完全可以被适用于本发明的领域，对于熟悉本领域的人员而言，可容易地实现另外的修改，因此在不背离权利要求及等同范围所限定的一般概念下，本发明并不限于特定的细节。Although the embodiment of the present invention has been disclosed as above, it is not limited to the use listed in the description and the implementation, it can be fully applied to the field of the present invention, and for those skilled in the art, it is easy to realize other Therefore, the invention is not limited to the specific details without departing from the general concept defined by the claims and their equivalents.
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