CN110129062A - A kind of algae competent cell soil conditioning agent producing process - Google Patents
A kind of algae competent cell soil conditioning agent producing process Download PDFInfo
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- CN110129062A CN110129062A CN201910516900.4A CN201910516900A CN110129062A CN 110129062 A CN110129062 A CN 110129062A CN 201910516900 A CN201910516900 A CN 201910516900A CN 110129062 A CN110129062 A CN 110129062A
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/14—Soil-conditioning materials or soil-stabilising materials containing organic compounds only
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2109/00—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE pH regulation
Abstract
The invention discloses a kind of algae competent cell soil conditioning agent producing process, the step of production technology, is as follows: the equipment in workshop, apparatus are carried out ultraviolet radiation for sterilizing and disinfecting；Then carry out carrying out the production of culture medium；Then to algae mother cell screening operation；Cell after screening is subjected to test tube cultivation, it will continue growing operation in the flask of cell inoculation to 100 milliliters after cultivating in previous step and be subject to light-illuminating, growing operation will be continued in the flask of the cell inoculation to 1,000 milliliters after cultivating in previous step and be subject to light-illuminating；Continue growing operation and supplemental oxygen in the flask of cell inoculation to 5,000 milliliters after cultivating in previous step and is subject to light-illuminating；The present invention, operation through the above steps can be quickly obtained a large amount of algae solution, improve production efficiency；And whole sterile working, pollution of the algae solution by bacterium is avoided, so that being guaranteed by the quality that algae solution configures gained conditioner.
Description
Technical field
The present invention relates to alga cells conditioner technical field, specially a kind of algae competent cell soil conditioner production
Technique.
Background technique
Chlorella Chlorella Vulgaris (being commonly called as green alga) Chlorophyta, eucaryote are a kind of spherical single-cell algaes
Class is one of life earliest on the earth, is a kind of efficient photosynthetic plant, a photoautotrophy growth and breeding.Chlorella
ChlofeUa Vulgaris is fresh water bead medicine, it is by sunlight, water and carbon dioxide, to divide out 4 every 24-28 hours
Solar energy is ceaselessly converted into the frond for containing multiple nutritional components, and rised in value by the vigorous fertility of a cell
In release a large amount of oxygen；Its vegetable protein content accounts for 50% or more, fat content 6-7%, amino acid composition also and
It is excellent.Containing several mineral materials and vitamin, cellulosic, folic acid, nucleic acid, key, magnesium, iron, zinc and several mineral materials vitamin
Deng balanced nutrition.Chlorella has the function of rehabilitating soil salinization of soil, and can degrade heavy metal pollution of soil, makes soil restoration
Normally.
Summary of the invention
The purpose of the present invention is to provide a kind of algae competent cell soil conditioning agent producing process, carry out to conventional apparatus
It improves, solves the problems in background technique.
To achieve the above object, the invention provides the following technical scheme: a kind of algae competent cell soil conditioner produces
Technique, it is characterised in that the step of production technology is as follows:
S1: the equipment in workshop, apparatus are subjected to ultraviolet radiation for sterilizing and disinfecting；
S2: the production of culture medium is carried out；
S3: to algae mother cell screening operation；
S4: the cell after screening in S3 step is subjected to test tube cultivation；
S5: growing operation will be continued in the flask of the cell inoculation to 100 milliliters after cultivating in S4 step and be subject to
Light-illuminating；
S6: growing operation will be continued in the flask of the cell inoculation to 1,000 milliliters after cultivating in S5 step and be subject to
Light-illuminating；
S7: growing operation will be continued in the flask of the cell inoculation to 5,000 milliliters after cultivating in S6 step and supplemented
Oxygen and it is subject to light-illuminating；
S8: growing operation will be continued in the flask of the cell inoculation to 20,000 milliliters after cultivating in S7 step and supplemented
Oxygen and it is subject to light-illuminating；
S9: growing operation will be continued in the reactor tank of the cell inoculation to eight hectoliters after cultivating in S8 step and mended
It is oxygenated, carbon dioxide and is subject to light-illuminating；
S10: algae solution sampling carries out the detection of Board Lot from S9 reactor tank；
S11: aforesaid operations are repeated, chlorella pyrenoidosa liquid, pit type ball algae solution, anabena liquid and small single qi algae solution is carried out
Individually preparation；
S12: chlorella pyrenoidosa liquid obtained by S12 step, pit type ball algae solution, anabena liquid and small single qi algae solution are pressed
It is even mixed according to ratio progress, sterile filling then is carried out to the algae solution after even mix.
Preferably, environment need to keep totally-enclosed, thermostatic control and germ-free condition in the workshop.
Preferably, environment temperature is maintained between 25 to 30 degrees centigrade in the workshop.
Preferably, when the S5 is inoculated with into S9 step the temperature of required water be maintained at 20 degrees Celsius to 28 it is Celsius
Between degree.
Preferably, the pH-value of required water is between six. five to eight when the S5 is inoculated with into S9 step.
Preferably, the light source of required light is electrodeless lamp source when the S5 is cultivated into S9 step, and illumination range is 2,000
Lux is between 5,000 luxs.
Preferably, the light source of required light is full spectrum Metal halogen lamp source, illumination range when the S5 is cultivated into S9 step
In 2,000 luxs between 5,000 luxs.
Compared with prior art, beneficial effects of the present invention are as follows:
One, the present invention is meeting list so that alga cells are able to carry out a large amount of breeding by the progress of S1 to S9 step
After the standard of bit quantity, that is, complete the cultivation of single algae solution；
Two, the present invention is by repeating above-mentioned steps chlorella pyrenoidosa liquid, pit type ball algae solution, fish raw meat are made respectively
Algae solution and small single qi algae solution, and the carry out of equal proportion is even mixed, and the cooperation of mixing algae solution can be realized, and finally carries out to mixing algae solution
Sterile filling, filling interior mixing algae solution can repair the salinization of soil of soil, and heavy metal pollution of soil of degrading makes soil
Restore normal.
Three, the operation of the present invention through the above steps, can be quickly obtained a large amount of algae solution, improve production efficiency；And
Whole sterile working avoids pollution of the algae solution by bacterium, guarantees the purity of algae solution, so that configuring gained conditioner by algae solution
Quality is guaranteed；
Detailed description of the invention
Fig. 1 is process flow chart of the invention.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
Referring to Fig. 1, the present invention provides a kind of technical solution: a kind of algae competent cell soil conditioning agent producing process,
It is characterized in that the step of production technology, is as follows:
S1: the equipment in workshop, apparatus are subjected to ultraviolet radiation for sterilizing and disinfecting, ultraviolet radiation for sterilizing and disinfecting is using appropriate
The ultraviolet light of wavelength can destroy the molecule of DNA (DNA) or RNA (ribonucleic acid) in microflora organisms cell
Structure causes growth cell death and (or) regenerative cell dead, achievees the effect that sterilizing.Ultraviolet Disinfection
It is to utilize the high efficiency of special designing, high intensity and long-life based on the basis of modern anti-epidemiology, medicine and photodynamics
UVC wave band ultraviolet lighting water jets, bacteriums various in water, virus, helminth, algae and other pathogens are directly killed,
Achieve the purpose that disinfection, avoid in the cultivation of cell, interfered by bacterium and normal growing operation can not be carried out, produces
Environment temperature is maintained between 25 to 30 degrees centigrade in workshop, is kept by temperature in zone of reasonableness, can make for
Good external condition is built in the breeding of cell, moreover it is possible to so that production operation personnel is kept good comfort level, so that product matter
Amount and working efficiency are guaranteed；
S2: carrying out the production of culture medium, and culture medium refers to supply microorganism, plant or animal (or tissue) growth and breeding
, the nutrient matrix as made of different nutriments formulated in combination.Generally all contain carbohydrate, nitrogen substance, inorganic salts
Several big substances such as (including microelement), vitamin and water.Culture medium had both been to provide cytotrophy and had promoted cell Proliferation
Basic substance and the living environment of cell growth and breeding, on culture medium, required alga cells carry out tentatively numerous
It grows；
S3: to algae mother cell screening operation, screening criteria is the size of cell dia, and preferably diameter range is three to five
The alga cells of micron；
S4: carrying out test tube cultivation for the cell after screening in S3 step, carbohydrate required for addition is grown in test tube, each
Kind minerals salt, inorganic matter and water, in test tube, the breeding of alga cells further progress；When alga cells unit in test tube
When quantity is greater than 1,000,000 every milliliter, invisible spectro alga cells are subjected to transfer and are seeded in bigger breeding space；
S5: growing operation will be continued in the flask of the cell inoculation to 100 milliliters after cultivating in S4 step and be subject to
Light-illuminating, in flask, the breeding of alga cells further progress is cooperated illumination, is grown using algae photoautotrophy, further
The supply of nutrition when improving breeding；S5 when being inoculated with into S9 step the temperature of required water be maintained at 20 degrees Celsius to 28 and take the photograph
Between family name's degree, by controlling water temperature between 20 degrees Celsius to 28 degrees Celsius, so that alga cells are able to maintain well
Activity so that breeding efficiently carried out；
S6: growing operation will be continued in the flask of the cell inoculation to 1,000 milliliters after cultivating in S5 step and be subject to
Light-illuminating, the breeding of alga cells further progress；
S7: growing operation will be continued in the flask of the cell inoculation to 5,000 milliliters after cultivating in S6 step and supplemented
Oxygen and it is subject to light-illuminating, the pH-value of S5 required water when being inoculated with into S9 step passes through control between six. five to eight
PH value provides a good living environment between six. five to eight, for alga cells；
S8: growing operation will be continued in the flask of the cell inoculation to 20,000 milliliters after cultivating in S7 step and supplemented
Oxygen and it is subject to light-illuminating, the light source of S5 required light when cultivating into S9 step is electrodeless lamp source, and illumination range is 2,000
Lux is between 5,000 luxs, is the technologies such as set electron, electromagnetism, vacuum in the world of one also known as " electromagnetic induction lamp "
The energy saving and environment friendly new light sources of forth generation have structure simple, electrodeless, special without filament, high photosynthetic efficiency, high-color rendering and long-life etc.
Point, so that algae is able to carry out photosynthesis, is realized autotrophy, provides enough nutrients for its breeding by the irradiation of light；
S9: growing operation will be continued in the reactor tank of the cell inoculation to eight hectoliters after cultivating in S8 step and mended
It is oxygenated, carbon dioxide and is subject to light-illuminating, alga cells is large number of in reactor tank, and when algae aerobic respiration is produced
When carbon dioxide needed for raw carbon dioxide can not meet its photosynthesis, guarantee its photosynthesis by increasing carbon dioxide
Be normally carried out；
S10: algae solution sampling carries out the detection of Board Lot from S9 reactor tank, and alga cells Board Lot is greater than when detection
2000000 every milliliter can be up to standard；
S11: aforesaid operations are repeated, chlorella pyrenoidosa liquid, pit type ball algae solution, anabena liquid and small single qi algae solution is carried out
Individually preparation；
S12: chlorella pyrenoidosa liquid obtained by S12 step, pit type ball algae solution, anabena liquid and small single qi algae solution are pressed
It is even mixed according to ratio progress, sterile filling then is carried out to the algae solution after even mix.
Referring to Fig. 1, the present invention provides another technical solution: a kind of algae competent cell soil conditioner production work
Skill, it is characterised in that the step of production technology is as follows:
S1: the equipment in workshop, apparatus are subjected to ultraviolet radiation for sterilizing and disinfecting, ultraviolet radiation for sterilizing and disinfecting is using appropriate
The ultraviolet light of wavelength can destroy the molecule of DNA (DNA) or RNA (ribonucleic acid) in microflora organisms cell
Structure causes growth cell death and (or) regenerative cell dead, achievees the effect that sterilizing.Ultraviolet Disinfection
It is to utilize the high efficiency of special designing, high intensity and long-life based on the basis of modern anti-epidemiology, medicine and photodynamics
UVC wave band ultraviolet lighting water jets, bacteriums various in water, virus, helminth, algae and other pathogens are directly killed,
Achieve the purpose that disinfection, avoid in the cultivation of cell, interfered by bacterium and normal growing operation can not be carried out, produces
Environment temperature is maintained between 25 to 30 degrees centigrade in workshop, is kept by temperature in zone of reasonableness, can make for
Good external condition is built in the breeding of cell, moreover it is possible to so that production operation personnel is kept good comfort level, so that product matter
Amount and working efficiency are guaranteed；
S2: carrying out the production of culture medium, and culture medium refers to supply microorganism, plant or animal (or tissue) growth and breeding
, the nutrient matrix as made of different nutriments formulated in combination.Generally all contain carbohydrate, nitrogen substance, inorganic salts
Several big substances such as (including microelement), vitamin and water.Culture medium had both been to provide cytotrophy and had promoted cell Proliferation
Basic substance and the living environment of cell growth and breeding, on culture medium, required alga cells carry out tentatively numerous
It grows；
S3: to algae mother cell screening operation, screening criteria is the size of cell dia, and preferably diameter range is three to five
The alga cells of micron；
S4: carrying out test tube cultivation for the cell after screening in S3 step, carbohydrate required for addition is grown in test tube, each
Kind minerals salt, inorganic matter and water, in test tube, the breeding of alga cells further progress；When alga cells unit in test tube
When quantity is greater than 1,000,000 every milliliter, invisible spectro alga cells are subjected to transfer and are seeded in bigger breeding space；
S5: growing operation will be continued in the flask of the cell inoculation to 100 milliliters after cultivating in S4 step and be subject to
Light-illuminating, in flask, the breeding of alga cells further progress is cooperated illumination, is grown using algae photoautotrophy, further
The supply of nutrition when improving breeding；S5 when being inoculated with into S9 step the temperature of required water be maintained at 20 degrees Celsius to 28 and take the photograph
Between family name's degree, by controlling water temperature between 20 degrees Celsius to 28 degrees Celsius, so that alga cells are able to maintain well
Activity so that breeding efficiently carried out；
S6: growing operation will be continued in the flask of the cell inoculation to 1,000 milliliters after cultivating in S5 step and be subject to
Light-illuminating, the breeding of alga cells further progress；
S7: growing operation will be continued in the flask of the cell inoculation to 5,000 milliliters after cultivating in S6 step and supplemented
Oxygen and it is subject to light-illuminating, the pH-value of S5 required water when being inoculated with into S9 step passes through control between six. five to eight
PH value provides a good living environment between six. five to eight, for alga cells；
S8: growing operation will be continued in the flask of the cell inoculation to 20,000 milliliters after cultivating in S7 step and supplemented
Oxygen and it is subject to light-illuminating, the light source of S5 required light when cultivating into S9 step is full spectrum Metal halogen lamp source, illumination range
In 2,000 luxs between 5,000 luxs, the light of the capable of emitting closest available light of Quan Guangpu Metal halogen lamp, because they
The light issued is composed entirely, that is, all light waves have.It is and least a portion of which includes the visible light of all colours
Ultraviolet light, so that algae is able to carry out photosynthesis, realizes autotrophy by the irradiation of light, provides enough support for its breeding
Point；
S9: growing operation will be continued in the reactor tank of the cell inoculation to eight hectoliters after cultivating in S8 step and mended
It is oxygenated, carbon dioxide and is subject to light-illuminating, alga cells is large number of in reactor tank, and when algae aerobic respiration is produced
When carbon dioxide needed for raw carbon dioxide can not meet its photosynthesis, guarantee its photosynthesis by increasing carbon dioxide
Be normally carried out；
S10: algae solution sampling carries out the detection of Board Lot from S9 reactor tank, and alga cells Board Lot is greater than when detection
2000000 every milliliter can be up to standard；
S11: aforesaid operations are repeated, chlorella pyrenoidosa liquid, pit type ball algae solution, anabena liquid and small single qi algae solution is carried out
Individually preparation；
S12: chlorella pyrenoidosa liquid obtained by S12 step, pit type ball algae solution, anabena liquid and small single qi algae solution are pressed
It is even mixed according to ratio progress, sterile filling then is carried out to the algae solution after even mix.
Working principle: the algae competent cell soil conditioning agent producing process in production, by S1 to S9 step into
Row, so that alga cells are able to carry out a large amount of breeding, after meeting the standard of Board Lot, that is, completes the training of single algae solution
It plants；Above-mentioned steps are repeated chlorella pyrenoidosa liquid, pit type ball algae solution, anabena liquid and small single qi algae solution are made respectively,
And the carry out of equal proportion is even mixed, and the cooperation of mixing algae solution can be realized, and finally carries out sterile filling to mixing algae solution, it is filling in
Mixing algae solution can repair the salinization of soil of soil, and heavy metal pollution of soil of degrading keeps soil restoration normal；By above-mentioned
The operation of step can be quickly obtained a large amount of algae solution, improve production efficiency；And whole sterile working, avoid algae solution by
The pollution of bacterium guarantees the purity of algae solution, so that being guaranteed by the quality that algae solution configures gained conditioner.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (7)
1. a kind of algae competent cell soil conditioning agent producing process, it is characterised in that the step of production technology is as follows:
S1: the equipment in workshop, apparatus are subjected to ultraviolet radiation for sterilizing and disinfecting；
S2: the production of culture medium is carried out；
S3: to algae mother cell screening operation；
S4: the cell after screening in S3 step is subjected to test tube cultivation；
S5: growing operation will be continued in the flask of the cell inoculation to 100 milliliters after cultivating in S4 step and is subject to light
Irradiation；
S6: growing operation will be continued in the flask of the cell inoculation to 1,000 milliliters after cultivating in S5 step and is subject to light
Irradiation；
S7: growing operation and supplemental oxygen will be continued in the flask of the cell inoculation to 5,000 milliliters after cultivating in S6 step
Be subject to light-illuminating；
S8: growing operation and supplemental oxygen will be continued in the flask of the cell inoculation to 20,000 milliliters after cultivating in S7 step
Be subject to light-illuminating；
S9: growing operation and delivery of supplemental oxygen will be continued in the reactor tank of the cell inoculation to eight hectoliters after cultivating in S8 step
Gas, carbon dioxide and it is subject to light-illuminating；
S10: algae solution sampling carries out the detection of Board Lot from S9 reactor tank；
S11: aforesaid operations are repeated, chlorella pyrenoidosa liquid, pit type ball algae solution, anabena liquid and small single qi algae solution is carried out individually
Preparation；
S12: to chlorella pyrenoidosa liquid obtained by S12 step, pit type ball algae solution, anabena liquid and small single qi algae solution according to than
Example progress is even mixed, then carries out sterile filling to the algae solution after even mix.
2. a kind of algae competent cell soil conditioning agent producing process according to claim 1, it is characterised in that: the life
Totally-enclosed, thermostatic control and germ-free condition need to be kept by producing environment in workshop.
3. a kind of algae competent cell soil conditioning agent producing process according to claim 1, it is characterised in that: the life
Environment temperature in workshop is produced to be maintained between 25 to 30 degrees centigrade.
4. a kind of algae competent cell soil conditioning agent producing process according to claim 1, it is characterised in that: the S5
The temperature of required water is maintained between 20 degrees Celsius to 28 degrees Celsius when being inoculated with into S9 step.
5. a kind of algae competent cell soil conditioning agent producing process according to claim 1, it is characterised in that: the S5
The pH-value of required water is between six. five to eight when being inoculated with into S9 step.
6. a kind of algae competent cell soil conditioning agent producing process according to claim 1, it is characterised in that: the S5
The light source of required light is electrodeless lamp source when cultivating into S9 step, illumination range 2,000 luxs to 5,000 luxs it
Between.
7. a kind of algae competent cell soil conditioning agent producing process according to claim 1, it is characterised in that: the S5
The light source of required light is full spectrum Metal halogen lamp source when cultivating into S9 step, and illumination range is in 2,000 luxs to five kiloluxs gram
Between this.
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ID=67577178
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110330983A (en) * | 2019-08-21 | 2019-10-15 | 阿尔格生命科学(江苏)有限公司 | A kind of active high-effect heavy metal degradation agent of algae |
| CN110476530A (en) * | 2019-08-21 | 2019-11-22 | 阿尔格生命科学(江苏)有限公司 | A kind of active high-effect salt affected soil Treatment process of algae |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110330983A (en) * | 2019-08-21 | 2019-10-15 | 阿尔格生命科学(江苏)有限公司 | A kind of active high-effect heavy metal degradation agent of algae |
| CN110476530A (en) * | 2019-08-21 | 2019-11-22 | 阿尔格生命科学(江苏)有限公司 | A kind of active high-effect salt affected soil Treatment process of algae |
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