CN101407772B - Method for cultivating photosynthetic bacteria - Google Patents
Method for cultivating photosynthetic bacteria Download PDFInfo
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- CN101407772B CN101407772B CN2008101374459A CN200810137445A CN101407772B CN 101407772 B CN101407772 B CN 101407772B CN 2008101374459 A CN2008101374459 A CN 2008101374459A CN 200810137445 A CN200810137445 A CN 200810137445A CN 101407772 B CN101407772 B CN 101407772B
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Abstract
The invention discloses a photosynthetic bacteria culture method, relating to bacteria culture methods. The method solves the problems of lower bacteria yield and high production cost in the existing photosynthetic bacteria cultivation. The photosynthetic bacteria culture method comprises following steps: a. a culture pool is positioned in a big-arch shelter, a pipeline mixer is arranged between the culture pool and a water pump, a culture medium is added into the pipeline mixer, and clean water is pumped into the culture pool by the water pump; and b. photosynthetic bacteria is inoculated ina culture liquid inside the culture pool for cultivation, thus finishing the cultivation of photosynthetic bacteria. The photosynthetic bacteria culture method adopts natural lighting and trace element oxygen supplying to cultivate photosynthetic bacteria, thus saving electricity charges by 70 percent and having low production cost as well as high photosynthetic bacteria yield.
Description
Technical field
The present invention relates to a kind of method for culturing bacteria.
Background technology
Photosynthetic bacterium is that a class can be carried out photosynthesis and the procaryotic general name of the special physiological monoid of non-oxygen-production belongs to the class in the hydrosphere microorganism, extensively is present in the natural various impounded body.Photosynthetic bacterial thallus is nontoxic, is rich in nutritive substance, protein content up to 60%～65%, contain physiologically active substances such as multiple vitamin B group, pantothenic acid, ubiquinone.Photosynthetic bacterium is widely used in aquaculture, plant husbandry, aspects such as sewage disposal.
At present, the cultivation of photosynthetic bacterium adopts the cultural method that adds the light source irradiation anaerobic condition, but exists bacterial growth slow, and bacteria concentration is low, OD
660=1.4 (optical density(OD) under the 660nm wavelength is 1.4), photosynthetic bacterium is produced effects low, and bacterial growth breeding need add light source, and the temperature control of cultivation pool also needs electrically heated intensification etc., and these all cause the photosynthetic bacterium production cost to increase.
Summary of the invention
The present invention seeks to for the cultivation that solves existing photosynthetic bacterium exists bacterium produce effects low and the high problem of production cost, and the cultural method of a kind of photosynthetic bacterium is provided.
The cultural method of photosynthetic bacterium is realized according to the following steps: a, the transparent cultivation pool of placement in the water white transparency booth, settle line mixer between transparent cultivation pool and the water pump, the RCVBN substratum is joined in the line mixer, with water pump clear water is pumped into transparent cultivation pool, extremely the nutrient solution degree of depth is 1.8～3.8m in the transparent cultivation pool; B, with OD
660=0.5～1.0 photosynthetic bacterium is inoculated in the nutrient solution of transparent cultivation pool, temperature be 20～35 ℃, dissolved oxygen concentration be under the condition of 0.2～1.0mg/L cultivating 1～3 day, promptly finish the cultivation of photosynthetic bacterium; Wherein the inoculum size of photosynthetic bacterium is 10%～30% (volume) among the step b.
Adopt natural lighting and micro-oxygen supply mode to cultivate photosynthetic bacterium among the present invention, can save 70% electricity charge expenditure, production cost is low, is applicable to the scale operation photosynthetic bacterium, and convenient management; Photosynthetic bacterium breeding among the present invention under the micro-oxygen supply state is quick, bacteria concentration height, OD
660=1.8, the photosynthetic bacterium height that produces effects; The water white transparency booth has the junk of isolation, guarantees that cultivation pool is in clean environment among the present invention, avoids the photosynthetic bacterium microbiological contamination in the cultivation pool, and the sunlight transmissivity is good, has insulation effect simultaneously; Transparent cultivation pool can make full use of natural lighting, and save energy has the good light transmittance energy, and tension, resistance to compression, antidetonation, intensity height.
Description of drawings
Fig. 1 is the growth curve collection of illustrative plates of photosynthetic bacterium in the embodiment six, and wherein ■ represents the growth curve of the photosynthetic bacterium that natural lighting and little oxygen condition are cultivated down, and ▲ expression adds the growth curve of the photosynthetic bacterium of cultivating under the light source irradiation anaerobic condition.
Embodiment
Technical solution of the present invention is not limited to following cited embodiment, also comprises the arbitrary combination between each embodiment.
Embodiment one: the cultural method of present embodiment photosynthetic bacterium is realized according to the following steps: a, the transparent cultivation pool of placement in the water white transparency booth, settle line mixer between transparent cultivation pool and the water pump, the RCVBN substratum is joined in the line mixer, with water pump clear water is pumped into transparent cultivation pool, extremely the nutrient solution degree of depth is 1.8～3.8m in the transparent cultivation pool; B, with OD
660=0.5～1.0 photosynthetic bacterium is inoculated in the nutrient solution of transparent cultivation pool, temperature be 20～35 ℃, dissolved oxygen concentration be under the condition of 0.2～1.0mg/L cultivating 1～3 day, promptly finish the cultivation of photosynthetic bacterium; Wherein the inoculum size of photosynthetic bacterium is 10%～30% (volume) among the step b.
The effect of water white transparency booth is to isolate junk in the present embodiment, guarantees that cultivation pool is in clean environment, avoids the photosynthetic bacterium microbiological contamination in the cultivation pool, and the sunlight transmissivity is good, has insulation effect simultaneously.
The standby power source of 40～100w incandescent light as rainy weather is installed above each cultivation pool in the water white transparency booth in the present embodiment.
Transparent cultivation pool adopts the water white transparency organic materials to make in the present embodiment, can make full use of natural lighting, has the good light transmittance energy, and tension, resistance to compression, antidetonation, intensity height.
Embodiment two: present embodiment and embodiment one are different be among the step a in the transparent cultivation pool nutrient solution degree of depth be 2.5m.Other step and parameter are identical with embodiment one.
In the present embodiment in the transparent cultivation pool of control the nutrient solution degree of depth help irradiate light and enter transparent cultivation pool bottom.
Embodiment three: present embodiment and embodiment one are different is that the inoculum size of photosynthetic bacterium among the step b is 20% (volume).Other step and parameter are identical with embodiment one.
Embodiment four: what present embodiment and embodiment one were different is with OD among the step b
660=0.7 photosynthetic bacterium is inoculated in the nutrient solution of transparent cultivation pool, temperature be 25 ℃, dissolved oxygen concentration be under the condition of 0.6mg/L cultivating 2.5 days, promptly finish the cultivation of photosynthetic bacterium.Other step and parameter are identical with embodiment one.
Cultured photosynthetic bacterium can directly be used after being emitted by rising pipe in the present embodiment, or by centrifugal, approach such as flocculation carry out the bacterium water sepn, makes and does the use of agent attitude.
Embodiment five: present embodiment and embodiment one are different is that the control of dissolved oxygen concentration among the step b adopts three kinds of micro-oxygen supply modes to realize, is respectively agitator and stirs oxygenation or aeration head aeration aerating; Wherein the mixing speed of agitator stirring oxygenation is 3～10r/min.Other step and parameter are identical with embodiment one.
Embodiment six: the cultural method of present embodiment photosynthetic bacterium is realized according to the following steps: a, the transparent cultivation pool of placement in the water white transparency booth, settle line mixer between transparent cultivation pool and the water pump, the RCVBN substratum is joined in the line mixer, with water pump clear water is pumped into transparent cultivation pool, extremely the nutrient solution degree of depth is 3m in the transparent cultivation pool; B, with OD
660=0.8 Rhodopseudomonas spheroides (Rhodobactersphaeroides) is inoculated in the nutrient solution of transparent cultivation pool, temperature be 25 ℃, dissolved oxygen concentration be under the condition of 0.8mg/L cultivating 2.5 days, promptly finish the cultivation of photosynthetic bacterium; Wherein the inoculum size of Rhodopseudomonas spheroides is 10% (volume) among the step b.
Photosynthetic bacterium grows under natural lighting and little oxygen condition soon in the present embodiment, by among Fig. 1 as can be seen, the photosynthetic bacterium growth speed of cultivating in the present embodiment is fast, bacteria concentration height, OD
660=1.8, than adding under the light source irradiation anaerobic condition photosynthetic bacterium of the cultivating height that produces effects.
Claims (2)
1. the cultural method of a photosynthetic bacterium, the cultural method that it is characterized in that photosynthetic bacterium is realized according to the following steps: a, the transparent cultivation pool of placement in the water white transparency booth, settle line mixer between transparent cultivation pool and the water pump, the RCVBN substratum is joined in the line mixer, with water pump clear water is pumped into transparent cultivation pool, extremely the nutrient solution degree of depth is 1.8～3.8m in the transparent cultivation pool; B, with OD
660=0.5～1.0 photosynthetic bacterium is inoculated in the nutrient solution of transparent cultivation pool, is that 20～35 ℃, dissolved oxygen concentration are to cultivate 1～3 day under little oxygen condition of 0.2～1.0mg/L in temperature, promptly finishes the cultivation of photosynthetic bacterium; Wherein, the cultivation of described photosynthetic bacterium is to cultivate under natural lighting, and photosynthetic bacterium is a Rhodopseudomonas spheroides among the step b, and inoculum size is 10～30% (volumes).
2. the cultural method of a kind of photosynthetic bacterium according to claim 1 is characterized in that the control of dissolved oxygen concentration among the step b adopts two kinds of micro-oxygen supply modes to realize, is respectively agitator and stirs oxygenation or aeration head aeration aerating; Wherein the mixing speed of agitator stirring oxygenation is 3～10r/min.
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| CN2008101374459A CN101407772B (en) | 2008-10-31 | 2008-10-31 | Method for cultivating photosynthetic bacteria |
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| CN2008101374459A CN101407772B (en) | 2008-10-31 | 2008-10-31 | Method for cultivating photosynthetic bacteria |
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| CN101407772B true CN101407772B (en) | 2011-02-02 |
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| CN102140429B (en) * | 2010-12-06 | 2012-12-26 | 青岛中仁药业有限公司 | Photosynthetic bacteria fermentation technology under natural ecological condition |
| CN108484237A (en) * | 2018-04-28 | 2018-09-04 | 沈阳师范大学 | A kind of preparation method and application of agricultural photosynthetic bacteria microorganism fertilizer |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2005033019A1 (en) * | 2003-09-30 | 2005-04-14 | O.K. Technologies, Llc | Autotrofic sulfur denitration chamber and calcium reactor |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2005033019A1 (en) * | 2003-09-30 | 2005-04-14 | O.K. Technologies, Llc | Autotrofic sulfur denitration chamber and calcium reactor |
Non-Patent Citations (2)
| Title |
|---|
| 杨绍斌.光合细菌大棚富集培养技术在养鱼中的应用.《淡水渔业》.2004,第34卷(第6期),37-38. * |
| 杨绍斌等.光合细菌简易富集培养技术及在北方养鱼中的作用.《水产养殖》.2005,第26卷(第1期),22-23. * |
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