CN101497473A - Aeration type photobioreactor and method of use thereof - Google Patents

Aeration type photobioreactor and method of use thereof Download PDF

Info

Publication number
CN101497473A
CN101497473A CN 200910047898 CN200910047898A CN101497473A CN 101497473 A CN101497473 A CN 101497473A CN 200910047898 CN200910047898 CN 200910047898 CN 200910047898 A CN200910047898 A CN 200910047898A CN 101497473 A CN101497473 A CN 101497473A
Authority
CN
China
Prior art keywords
aeration
annular
photobioreactor
liquid
pipe
Prior art date
Application number
CN 200910047898
Other languages
Chinese (zh)
Inventor
蔡志武
Original Assignee
上海大祺环保工程有限公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 上海大祺环保工程有限公司 filed Critical 上海大祺环保工程有限公司
Priority to CN 200910047898 priority Critical patent/CN101497473A/en
Publication of CN101497473A publication Critical patent/CN101497473A/en

Links

Classifications

    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W10/00Technologies for wastewater treatment
    • Y02W10/10Biological treatment of water, waste water, or sewage
    • Y02W10/15Aerobic processes

Abstract

The invention relates to the field of microalgae cultivation engineering, in particular to an aeration type optical bioreactor for cultivating microalgae and a method for applying the same. The bioreactor comprises an annular wading pool, a liquid inlet main pipe, an annular liquid replenishing pipe, an atomization nozzle, a hot water pipe coil, an air inlet main pipe, an annular air inlet trunk pipe, an air distribution branch pipe and an aeration device. The bioreactor is characterized in that density difference between microbubbles generated by the aeration device through aeration and peripheral algae liquid promotes the floating of the microbubbles and the declining of the algae liquid; countless gas liquid generated by the annular wading pool circulates and flows up and down so that the upper layer and the lower layer of the algae liquid are mixed; the floating of the bubbles strengthens desorption of dissolved oxygen in the algae liquid; oxygen is taken to the liquid level by the bubbles and is released; and carbon dioxide gas is aerated and simultaneously dissolved in the algae liquid to provide a carbon source for the growth of the microalgae. The invention combines the advantages of an open type optical bioreactor and a closed type optical bioreactor to form a novel closed type optical bioreactor which can use the carbon dioxide gas as the carbon source and cultivate the microalgae in low cost and large scale.

Description

曝气式光生物反应器及其应用方法 Aeration photobioreactor and application method

[技术领域] [Technical Field]

本发明涉及微藻培养工程领域,具体地说是一种培养微藻的曝气式光生物反应器及其应用方法。 The present invention relates to microalgae culture engineering, specifically aeration photobioreactor and its application method for culturing microalgae. [背景技术] [Background technique]

微藻能有效利用光能、二氧化碳和无机盐,合成脂肪、蛋白质、多糖以及多种高附加值的生物活性物质。 Microalgae effective use of solar energy, carbon dioxide and inorganic salts, synthetic fats, proteins, polysaccharides, and a variety of biologically active substances of high added value. 随着人类对微藻认识的不断加深,开发和研制新型高效光生物反应器及其培养方法,已成为微藻生物工程技术的一个重要组成部分。 As human beings continue to deepen the understanding of microalgae, research and development of new and efficient photobioreactor and its culture method, it has become an important part of microalgae bio-engineering technology.

目前,微藻培养主要有开放式和封闭式两种光生物反应器。 Currently, microalgae culture mainly two kinds of open and closed photobioreactor. 开放式光生物反应器主要有四种类型:浅水池、循环池、跑道池、池塘。 Open photobioreactor There are four main types: shallow pool, circulation tank, raceway pond, the pond. 其中,最 One of the most

典型、最常用的开放池培养系统是Oswald (1969)设计的跑道池反应器, 该类培养系统实际上就是占地面积上千平方米,培养液深度为20厘米左右的环形浅池;以自然光为光源和热源,依靠桨轮(paddle wheel)或者旋转臂(rotating arm)的转动,使培养液在池内混合、循环,防止藻体沉淀并提高藻体细胞的光能利用率。 Typically, the most commonly used cell culture system is open Oswald (1969) of the raceway pond reactor design, such culture systems actually covers thousands of square meters, the culture was about 20 cm depth of annular shallow pool; natural light of light and heat, the paddle wheel relying (paddle wheel) or the rotary arm (rotating arm) is rotated, the culture was mixed in the pool, circulation and prevent precipitation of algae and improve energy efficiency algae cells. 开放式光生物反应器虽然构建简单、成本低廉、操作简便,但其存在易受污染、培养条件不稳定等缺点,只能用于螺旋藻、小球藻及盐藻等少数能耐受极端环境的微藻培养。 While constructing open photobioreactors simple, low cost, easy to operate, but its presence susceptible to contamination, culture conditions and other shortcomings is unstable, can only be used Spirulina, Chlorella and Dunaliella can withstand extreme environments and a few the microalgae culture. 而封闭式反应器培养条件稳定,可无菌操作,易进行高密度培养,己成为今后的发展方向。 Closed Reactor conditions and stable culture, aseptic operation can be easily carried out high density culture, has become the future development direction. 与开放式光生物反应器相比,封闭式光生物反应器具有以下优点:① 无污染,能实现单种、纯种培养;②培养条件易于控制;③培养密度高,易收获;④适合于所有微藻的光自养培养,尤其适合于微藻代谢产物的生产;⑤有较高的光照面积与培养体积之比,光能和二氧化碳利用率较高。 Compared with open photobioreactor, a closed photobioreactor has the following advantages: ① pollution, to achieve a single, pure culture; ② culture conditions easy to control; ③ high density culture, and easy harvesting; ④ adapted to All light autotrophic culture of microalgae, particularly suitable for the production of microalgae metabolites; ⑤ higher illumination area to volume ratio of the culture, high energy efficiency, and carbon dioxide. 因此,近年来在国内外封闭式光生物反应器的研制和开发利用较快,有些己实现了微藻的高密度商业化培养。 Therefore, in recent years in the development and exploitation of domestic and foreign closed photobioreactor faster, some had to achieve a high density of commercial cultivation of microalgae. 目前封闭式光生物反应器有:管道式、平板式、柱状气升式、搅拌式发酵罐、立式吊袋、浮式薄膜袋等。 Currently there is a closed photobioreactor: pipeline, plate, columnar airlift, stirred fermentor, vertical punching bags, bags floating film.

现有的封闭式光生物反应器或多或少易造成溶氧蓄积、藻体粘壁、高密度培养时二氧化碳供应不足、流动死角、设备造价昂贵等各种问题,除了管道式反应器外,基本都处于实验室规模,容积不超过1个立方米。 Existing closed photobioreactor or less easily lead to accumulation of dissolved oxygen, algae sticky wall, inadequate supply of carbon dioxide is high density culture, the flow dead, expensive equipment and other problems, in addition to the pipe reactor, basically at the laboratory scale, capacity not more than one cubic meter. 而管道式光生物反应器目前报道的年产虽己达上百吨藻粉,但该设备的造价及维修成本很高,很难普及。 The annual output of pipeline photobioreactor reported so far though has reached hundreds of tons of algae powder, but the high cost and maintenance cost of the equipment, it is difficult to spread.

实际上,无论光生物反应器采用何种设计形式,开发一种成功的培养微藻的封闭式光生物反应器,均应遵循下列原则:(l)高光照表面积与体积之比;(2)较高的气体交换效率;(3)适合的循环方式;(4)改善光的传播途径、分配和质量;(5)防止有害次生代谢产物的积累;(6)较易实现培养条件的优化控制;(7)尽量降低制造成本。 In fact, no matter what kind of optical design forms bioreactor employed to develop a successful culturing microalgae closed photobioreactor, should the following principles: (l) a high surface area to volume ratio of illumination; (2) higher gas exchange efficiency; (3) a suitable cyclic manner; and (4) improve light transmission, distribution, and quality; (5) preventing the harmful accumulation of secondary metabolites; (6) easier to achieve optimization of culture conditions control; (7) as far as possible to reduce manufacturing costs. 可以预见,那种成本低、容积大、 设计更为合理的封闭式光生物反应器应是开发的重点和发展方向。 It is foreseeable that the kind of low cost, high volume, more rational design of a closed photobioreactor development should be the focus and direction.

微藻在培养过程中所供给的碳源最常用的为碳酸氢钠,该成本占原料成本的40〜70%,若以二氧化碳为碳源代替碳酸氢钠,其碳源利用率高,价格便宜,可以大幅度降低成本,理论上碳源的成本可降低90%左右。 Microalgae carbon source supplied during cultivation of the most commonly used is sodium bicarbonate, the cost accounting 40~70% of the cost of raw materials, if the carbon dioxide as the carbon source in place of sodium bicarbonate, its high carbon utilization, cheap , can significantly reduce costs, in theory, can reduce the cost of carbon is about 90%. 以二氧化碳气体为碳源的光生物反应器必须保证二氧化碳气体被良好地分散与吸收,而微藻产生的氧气被有效地解吸,以及培养液的均布与良好混合。 Carbon dioxide gas as a carbon source photobioreactor must ensure that the carbon dioxide gas is well dispersed and absorbed, while oxygen is produced by microalgae desorbed efficiently, and the uniform well-mixed culture liquid. [发明内容]本发明的目的在于克服上述现在技术存在的缺陷及不足,开发一种投资少、操作简单,能充分利用太阳光能,反应器结构简洁、实用,并能以二氧化碳气体为碳源,进行大规模培养微藻的曝气式光生物反应器。 [Disclosure of Invention] The present invention is now to overcome the above defects and deficiencies exist in the art to develop a small investment, easy to operate, can make full use of solar energy, the reactor structure is simple, practical, and capable of a carbon dioxide gas as a carbon source , massive aeration photobioreactor for culturing microalgae.

曝气式光生物反应器包括环状浅水池1、隔离墙2、排液管3、排污管4、扁钢5、农用薄膜6、天窗7、透光盖板8、环状补液管9、雾化喷嘴10、进液总管11、环状进气干管12、进气总管13、布气支管14、曝气装置15、热水盘管,其特征在于:反应器为一环状浅水池1,环状浅水池中部设有一道两端开放的隔离墙2,曝气式光生物反应器右部下端设有排液管3,反应器的底端设有排污管4,曝气式光生物反应器顶端设有起支撑作用的扁钢5,扁钢的两端与池壁上端连接固定,扁钢5上设有农用薄膜6覆盖整个环状浅水池1,成为封闭式光生物反应器,在农用薄膜6上设有天窗7,天窗上设有透光盖板8;扁钢5下面设有围绕隔离墙2的环状补液管9, 补液管的下部设有雾化喷嘴10,环状补液管9与进液总管ll相连通;环状浅水池1的底部、侧壁以及隔离墙2的侧壁上设有热水盘管;环状浅 Aeration photobioreactor comprises an annular shallow pool 1, the wall 2, the drain tube 3, sewage pipes 4, 5 flat, agricultural films 6, 7 skylights, light-transmissive cover plate 8, the annular fluid pipe 9, atomizing nozzle 10, inlet manifold 11, an annular intake main pipe 12, an intake manifold 13, air distribution manifold 14, the aeration device 15, a hot water coil, which is characterized in that: an annular reactor is shallow pool 1, is provided with a central annular shallow pool wall 2 open at both ends, the lower end of the photobioreactor aeration right portion 3 is provided with liquid discharge pipe, the bottom end of the reactor 4 is provided with outfall, light aeration the bioreactor is provided to the top play a supportive role flat 5, and an upper end connected and fixed to the wall of the flat ends, with an agricultural film 6 covers the entire upper annular flat shallow pool 1 5, be closed photobioreactor in the agricultural film 6 is provided with skylights 7, 8 provided on the light-transmitting cover skylight; flat bar 5 is provided below the annular wall surrounding the infusion tube 2 9, the lower portion of the tube is provided with a fluid atomizing nozzle 10, ring shaped tube 9 and the fluid inlet manifold communicating ll; a hot water coil is provided on a side wall of the bottom annular shallow pool 1, the wall 2 and the side wall; a shallow annular 池1 的中下部设有平行的围绕隔离墙2的环状进气干管12,环状进气干管与进气总管13相连通,环状进气干管12上与布气支管14连接,布气支管与池底平行,并与池壁垂直,布气支管14的端部分别固定在池壁或隔离墙上; 布气支管14上设有曝气装置15。 1 is provided with a lower portion of the tank is connected to the gas distribution manifold 14 on the annular wall 12 surrounding two parallel intake main pipe 12, and the annular intake main pipe 13 communicates the intake manifold, the intake annular trunk , gas distribution manifold and parallel to the bottom, and the wall perpendicular to the end portions of the gas distribution manifold 14 are fixed on the wall or the wall; aeration apparatus 15 is provided with a gas distribution manifold 14.

所述的曝气装置15为微孔曝气管或穿孔曝气管或橡胶膜片微孔曝气器或钟罩式刚玉微孔曝气器或微孔圆盘曝气器或微孔圆盔曝气器或长条板形曝气器或双螺旋曝气器或旋混式曝气器或散流式曝气器或曝气软管或可变微孔曝气器或OXAZUR空气扩散器或单孔膜空气扩散器。 The aeration means 15 is a microporous or perforated aeration tube aeration tubes or rubber diaphragm porous aerator or aeration microporous bell corundum disc or microporous diffusers circular helmet or microporous aerator or aeration elongated plate-shaped or twin screw mixing aerator or aeration or spin diffuser aerator or aeration hoses or variable porous diffusers or diffuser or air OXAZUR membrane air diffuser hole.

7所述的透光盖板8采用钢化玻璃板或有机玻璃板或聚碳酸酯板或覆有农膜的扁钢框。 The light transmitting cover 7, 8 tempered glass plate or organic glass plate or a polycarbonate plate or flat steel frame covered with plastic sheeting.

所述的热水盘管为无规共聚聚丙烯PP-R管或交联聚乙烯PE-X管或耐高温聚乙烯PE-RT管或铝塑复合管XPAP或耐冲击共聚聚丙烯PP-C(B)管或聚丁烯PB管或铜管。 The hot water coil is PP-R polypropylene random copolymer or cross-linked polyethylene tube PE-X or refractory polyethylene tube PE-RT pipes or plastic or composite pipe XPAP impact polypropylene copolymer PP-C (B) or polybutylene pipe PB pipe or brass.

曝气式光生物反应器的应用方法,其特征在于进气总管13接过滤空气与二氧化碳钢瓶气或二氧化碳工业废气的混合气,依靠曝气实现微藻培养中的气体交换;曝气装置曝气的气流方向与垂直向上方向的夹角为90〜160 度之间,曝气产生的微小气泡与周围藻液的密度差,促使气泡上浮以及藻液下降,由此环状浅水池内产生无数个气液上下循环流动,使上下层藻液进行混合,即气液密度差导致对流混合,而气泡的上浮强化了藻液中溶氧的解吸,氧气被气泡带至液面而释放,并且二氧化碳气体经曝气后也同时溶解在藻液中,为微藻的生长提供碳源;当曝气式光生物反应器中积累的氧气浓度达到一定水平时,升高或平移透光板进行通风及散热,同时加大反应器进气总管的进气量,用于加快排出氧气的速度,同时保证反应器中微小的正压,防止外界空气中的微 Application of the method aeration photobioreactor, characterized in that the intake manifold 13 connected to the filtered air with carbon dioxide or a mixed gas of carbon dioxide gas cylinder industrial gas, relying on aeration to achieve gas exchange microalgae culture; aerator aeration airflow direction perpendicular angle upward direction between 90~160 degrees, the density of fine bubbles and the surrounding liquid algae aeration difference generated bubble causes liquid drops floating algae and thereby produce numerous cyclic shallow gas tanks circulating fluid down the upper and lower alginate solution are mixed, i.e., the density difference leads to convective mixing liquid, the bubble floats reinforced alginate dissolved oxygen desorption liquid, with bubbles of oxygen is released to the liquid surface, and the carbon dioxide gas through the after aeration also dissolved in the alginate solution, providing a carbon source for the growth of microalgae; when aeration photobioreactor accumulated oxygen concentration reaches a certain level, the light-transmitting plate or translating increased ventilation and heat dissipation, the reactor while increasing the intake air amount of the intake manifold for discharging oxygen speed, while maintaining a slight positive pressure in the reactor, to prevent outside air micro 物及灰尘进入反应器;在光生物反应器中营养盐浓度下降后,通过环状补液管9及雾化喷嘴10,均匀地向光生物反应器中补加营养液;在光生物反应器中由于通入大量二氧化碳导致培养液的pH值下降后,通过环状补液管9及雾化喷嘴10,均匀地喷洒碱性溶液来提高培养液的pH值;在光生物反应器中培养液温度下降至微藻适宜生长的温度下限后,在热水盘管中通入热水对培养液进行加热及保温。 Material and dust into the reactor; in the photobioreactor after nutrient concentration decreased, the tube 9 and through the annular fluid atomizing nozzle 10, the light is uniformly bioreactor supplemented nutrient solution; in the photobioreactor in Since a large amount of carbon dioxide into the culture medium results in reduction of the pH, the pipe 9 and through the annular fluid atomizing nozzle 10, an alkaline solution is sprayed uniformly to raise the pH of the culture medium; medium temperature drop in the photobioreactor the lower limit to the temperature suitable for the growth of microalgae, the hot water in the hot water was passed through the coil of the heating medium and incubated.

所述的二氧化碳废气为来自电厂、钢铁厂、水泥厂、陶瓷厂、化工厂的烟道气,以及生物发酵厂的二氧化碳工艺废气。 The carbon dioxide is flue gas from power plants, steel mills, cement, ceramics, chemical plants flue gas, carbon dioxide plants and fermentation process emissions. 所述的微藻为淡水微藻或咸湖水微藻或海水微藻,类别为金藻或绿藻 The fresh water microalgae microalgae or microalgae salty lake or sea algae, golden algae or green algae category

或硅藻或蓝藻或红藻,优选为螺旋藻或葡萄藻或小球藻或球等鞭金藻3011 或绿色巴夫藻。 Or blue-green algae or red algae or diatoms, preferably spirulina or chlorella algae or grapes such as ball or Isochrysis or Pavlova 3011. 所述的喷洒的碱性溶液为低浓度的氢氧化钠溶液、氢氧化钾溶液,或者高浓度的碳酸钠溶液、碳酸钾溶液。 The alkaline solution is sprayed sodium carbonate solution having a low concentration of sodium hydroxide solution, potassium hydroxide solution, or a high concentration of potassium carbonate solution.

由于本发明培养微藻的生产装置依靠曝气实现微藻培养中的气体交换,省去了庞大的桨轮或旋转臂,整个反应器能够方便地用农用薄膜6完整地覆盖。 Since the present invention is the production of microalgae culture device relies implemented microalgae culture aeration gas exchange, eliminating the need for a large paddle wheel or rotating arms, the whole reactor Agricultural film 6 can be easily covered completely with. 这里的农用薄膜选用有利于光合作用的薄膜,例如能防止在薄膜上形成雾滴而降低透光率的无滴薄膜,以及利用光学原理将光转换成对微藻光合作用有利的转光膜等。 Here agricultural film selection favor photosynthesis film, for example, a thin film can be prevented without dripping droplets formed on the film to reduce light transmittance, the use of optics and converting light microalgae pair advantageous conversion film like photosynthesis . 在农用薄膜6的中部留有天窗,天窗上盖有带升降或平移装置的透光盖板8。 Agricultural film 6 in the middle of the left skylight, sunroof or pan with lift covered with a light-transmitting cover means 8. 在炎热的夏天,可在农用薄膜6上再覆盖一层遮光膜,防止光照过强而抑制甚至损害微藻的生长,同时降低反应器内温度。 In hot summer may be further coated on the light-shielding film agricultural film layer 6, light is too strong to prevent damage or even suppress the growth of algae, while reducing the temperature in the reactor.

本曝气式光生物反应器设有pH电极、溶氧电极、温度传感器、液位计,通过变送器把信号送至计算机中进行显示。 This aeration photobioreactor with pH electrodes, DO electrodes, temperature sensors, liquid level, the signal sent to the computer is displayed by the transmitter. 同时,反应器的进气总管、进液总管、排液管上都设有手动及自动阀门,反应器天窗上的透光盖板也设有手动及自动升高或平移装置,在计算机对输入信号进行处理后, 根据微藻的培养工艺,可自动调节各阀门及天窗的开度。 At the same time, it is provided with an intake manifold of the reactor, the inlet manifold, the drain tube manual and automatic valves, a light-transmitting cover on the reactor sunroof with manual and automatic pan raising means or in a computer input after the signals are processed, the process according to culture microalgae, can automatically adjust the degree of opening of each valve and skylights. 另外,藻液浓度的检测既可以从反应器的天窗口对藻液取样分析其OD值,也可以在反应器内部的藻液中安装OD传感器,自动连续检测OD值。 Further, the algae concentration detection may be analyzed OD value was sampled from algae reactor day window may be mounted in the sensor OD algae solution inside the reactor, the automatic and continuous detection OD.

本发明结合开放式与封闭式光生物反应器各自优点,形成一种能以二氧化碳气体为碳源,低成本、大规模培养微藻的新型封闭式光生物反应器,这将在推进微藻养殖的产业化过程中发挥重要作用。 The present invention combines the open vs. closed photobioreactor respective advantages, formation of a new closed photobioreactor capable of one kind of carbon dioxide gas as a carbon source, a low-cost, large-scale cultivation of microalgae, which will promote the culture of microalgae the process of industrialization plays an important role. [附图说明]图1为本发明培养微藻的曝气式光生物反应器的结构示意图。 [BRIEF DESCRIPTION OF DRAWINGS] FIG 1 is a schematic structural aeration photobioreactor for culturing microalgae of the present invention.

参见图l, l为环状浅水池,2为隔离墙,3为排液管,4为排污管,5 为扁钢,6为农用薄膜,7为天窗,8为透光盖板,9为环状补液管,10为雾化喷嘴,ll为进液总管,12为环状进气干管,13为进气总管,14为布气支管,15为曝气装置。 Referring to Figure l, l is an annular shallow pool, the wall 2, 3 of the drainage pipes, sewage pipes 4, 5 is flat, agricultural film 6, 7 for the skylight, the light-transmitting plate 8, 9 annular fluid tube 10 for the atomizing nozzle, ll to the inlet manifold, the intake 12 of the annular main pipe, the intake manifold 13, 14 is a gas distribution manifold 15 of the aeration device. [具体实施方式] [Detailed ways]

以下结合附图和实施例对本发明作进一步说明。 The present invention will be further described in conjunction with the accompanying drawings and embodiments. 本发明的制造技术对本专业的人来说是容易实施的。 Fabrication techniques of the present invention is a professional person easy to implement.

如图1,培养球等鞭金藻3011的曝气式光生物反应器为环状浅水池1,围墙由砖块砌筑,表面用水泥抹平,环状浅水池1的池底则铺设复合土工膜,池中用砖砌一道两端开放的隔离墙2。 1, Isochrysis algae culture aeration photobioreactor annular shallow pool 3011 is 1, the masonry brick walls, cement smooth surface, a bottom annular shallow pool is laid composite geomembrane, open at both ends of the pool with a brick in the wall 2. 池的宽度为4米,长度为100 米,深度为60厘米,隔离墙2高度为70厘米。 Cell width of 4 m, a length of 100 meters and a depth of 60 cm wall height of 70 cm 2. 曝气式光生物反应器右部下端设有排液管3,底部设有排污管4。 Lower aeration photobioreactor right portion 3 is provided with discharge pipes, sewage pipes is provided with a bottom 4. 曝气式光生物反应器顶部设有一排起支撑作用的拱形扁钢5,扁钢的两端固定在池壁上端。 Top aeration photobioreactor with a row of arcuate flat play a supportive role 5, fixed to the upper ends of the wall flat. 扁钢上采用农用薄膜6完整地覆盖整个环状浅水池1,成为封闭式光生物反应器,在农用薄膜6的中部设有天窗7,天窗上盖有透光盖板8。 Agricultural film 6 completely covers the entire annular shallow pool 1, a closed photobioreactor be employed on flat, skylights 7 of 6 in the central agricultural film, the light-transmitting plate 8 covered with skylights. 扁钢5下面固定有围绕隔离墙2的环状补液管9,补液管的下部开有许多雾化喷嘴10,环状补液管9 与进液总管11相连通。 Below about 5 fixed to flat annular wall 9 of the second fluid tube, a lower tube opening many fluid atomizing nozzle 10, an annular fluid pipe 9 through the inlet manifold 11 is connected. 环状浅水池1的底部、侧壁以及隔离墙2的侧壁上铺有热水盘管,管材优选为PP-R管。 Annular bottom shallow pool 1, the side walls and the wall 2 of the side wall plated with a hot water coil, preferably a pipe PP-R pipe. 环状浅水池1的中下部固定有2根平行的围绕隔离墙2的环状进气干管12,环状进气干管均与进气总管13相连通,进气总管13接过滤空气与附近热电厂的二氧化碳烟道气的混合气。 1 the lower annular shallow pool about two fixed parallel to the annular wall 2 of the intake main pipe 12, an annular intake main pipe 13 are connected to the intake manifold through the intake manifold 13 is attached to the air filter a mixed gas of carbon dioxide in the vicinity of thermal power plant flue gas. 环状进气干管12上与数根布气支管14连接,布气支管与池底平行,并与池壁垂直,布气支管的端部分别固定在池壁或隔离墙上;布气支管14上设有多个硅橡胶微孔曝气管15,该管式曝气器曝气的气流方向均为水平向前。 Dry annular intake pipe connected to the number of the air distribution manifold 14 is 12, and the gas distribution manifold parallel to the bottom, and the wall with vertical air distribution manifold end portions respectively fixed on the wall or the wall; air distribution manifold a plurality of porous silicone rubber 15 on the aeration tube 14, the gas flow direction of the tubular aerator aeration is horizontal forward. 曝气产生的上浮气泡使上下层藻液进行混合,而气泡的上浮强化了藻液中溶氧的解吸,氧气被气泡带至液面而释放,并且二氧化碳气体经曝气后也同时溶解在藻液中,为微藻的生长提供碳源。 Floating bubble aeration generated by the upper lower alginate solution were mixed, and bubbles floating algae solution strengthened desorption of dissolved oxygen, the oxygen level is to release the blister strip, and after carbon dioxide gas is also dissolved in the aeration alginate solution, providing a carbon source for the growth of microalgae. 在光生物反应器中营养盐浓度下降后,通过环状补液管9及雾化喷嘴10,均匀地向光生物反应器中补加营养液;在光生物反应器中由于通入大量二氧化碳导致培养液的pH值下降后,通过环状补液管9及雾化喷嘴10,均匀地喷洒低浓度的氢氧化钠溶液来提高培养液的pH值。 In the photobioreactor after nutrient concentration decreased, the tube 9 and through the annular fluid atomizing nozzle 10, the light is uniformly bioreactor supplemented nutrient solution; in the photobioreactor lot of carbon dioxide due to lead into the culture after the pH of the solution is lowered, the pipe 9 and through the annular fluid atomizing nozzle 10, sprayed uniformly low concentrations of sodium hydroxide solution to raise the pH of the culture medium. 在光生物反应器中培养液温度下降至15'C后, 在热水盘管中通入5(TC热水把培养液加热至25'C并保温。 Cultured in a photobioreactor in the liquid temperature decreased to 15'C, the hot water passed through the coil 5 (TC hot water to the culture and incubation was heated to 25'C.

整个曝气式光生物反应器用农用薄膜6完整地覆盖,形成封闭式光生物反应器。 Entire light aeration bioreactor was complete covering agricultural film 6, forming a closed photobioreactor. 这里的农膜选用长寿命的聚氯乙烯无滴薄膜,防止薄膜上形成雾滴而降低透光率。 Here the choice of plastic sheeting long life drip-free PVC film, light transmittance is reduced to prevent the droplets formed on the film. 在薄膜的中部开有天窗,天窗上盖有带升降装置的聚碳酸酯透光盖板8,当曝气式光生物反应器中积累的氧气浓度达到一定水平时,升高透光板进行通风及散热。 In the middle of the film to open sunroof, skylight covered with translucent polycarbonate lifting device with the cover 8, when the aeration photobioreactor accumulated oxygen concentration reaches a certain level, the light-transmitting plate is increased ventilation and heat dissipation. 在炎热的夏天,可在农用薄膜6上再覆盖一层遮光膜,防止光照过强而抑制甚至损害微藻的生长,同时降低反应器内温度。 In hot summer may be further coated on the light-shielding film agricultural film layer 6, light is too strong to prevent damage or even suppress the growth of algae, while reducing the temperature in the reactor.

曝气式光生物反应器中装有pH电极、溶氧电极、温度传感器、液位计等,通过变送器把信号送至计算机中进行显示。 Light aeration bioreactor with a pH electrode, oxygen electrode, temperature sensor, level meter, etc., the signal sent to the computer is displayed by the transmitter. 同时,反应器的进气总管、进液总管、排液管上都装有手动闸阀,藻液浓度的检测可以从反应器的天窗口对藻液取样分析其OD值。 Meanwhile, the intake manifold of the reactor, the inlet manifold, the liquid discharge tubes are equipped with manual valve, algae concentration detection window from the day the reactor was sampled and analyzed for algal OD values.

ii ii

Claims (7)

1、一种曝气式光生物反应器包括环状浅水池(1)、隔离墙(2)、排液管(3)、排污管(4)、扁钢(5)、农用薄膜(6)、天窗(7)、透光盖板(8)、环状补液管(9)、雾化喷嘴(10)、进液总管(11)、环状进气干管(12)、进气总管(13)、布气支管(14)、曝气装置(15)、热水盘管,其特征在于:反应器为一环状浅水池(1),环状浅水池(1)中部设有一道两端开放的隔离墙(2),曝气式光生物反应器右部下端设有排液管(3),反应器的底端设有排污管(4),曝气式光生物反应器顶端设有扁钢(5),扁钢的两端与池壁上端连接,扁钢(5)上设有覆盖整个环状浅水池(1)的农用薄膜(6),在农用薄膜(6)上设有天窗(7),天窗(7)上设有透光盖板(8);扁钢(5)下面设有围绕隔离墙(2)的环状补液管(9),补液管的下部设有雾化喷嘴(10),环状补液管(9)与进液总管(11)相连通;环状浅水池(1)的底部、侧壁以及隔离墙(2)的侧壁上 An aeration photobioreactor comprises an annular shallow pool (1), the wall (2), the drain tube (3), sewer (4), flat bar (5), agricultural films (6) skylight (7), the translucent cover plate (8), an annular fluid tube (9), the atomizing nozzle (10), inlet manifold (11), an annular air intake trunk (12), an intake manifold ( 13), gas distribution manifold (14), aeration means (15), a hot water coil, which is characterized in that: an annular reactor is shallow pool (1), an annular shallow pool (1) is provided with a central two the open end wall (2), the lower end of the photobioreactor aeration unit is provided with a right drain pipe (3), the bottom end of the reactor is provided with outfall (4), aeration is provided photobioreactor top there are flat (5), with the upper end of the wall is connected to both ends of the flat steel, provided with an annular shallow pool covering the entire (1) agricultural film (6) on the flat bar (5), disposed on the agricultural film (6) sunroof (7), it is provided with the sunroof cover lens (8) (7); flat bar (5) provided below the annular fluid tube (9) surrounding the wall (2), the lower portion of the fluid pipe is provided atomizing nozzle (10), an annular fluid tube (9) and inlet manifold (11) communicating; upper annular sidewall bottom shallow pool (1), the side walls and the wall (2) 有热水盘管;环状浅水池(1)的中下部设有平行的围绕隔离墙(2)的环状进气干管(12),环状进气干管(12)与进气总管(13)相连通,环状进气干管(12)上与布气支管(14)连接,布气支管(14)与池底平行,并与池壁垂直,布气支管(14)的端部分别固定在池壁或隔离墙上;布气支管(14)上设有曝气装置(15)。 Hot water coil; cyclic shallow pool (1) is provided parallel to the lower annular surrounding wall (2) intake trunk (12), an annular intake trunk (12) and the intake manifold (13) communicating the annular intake main pipe (12) connected to the gas distribution manifold (14), gas distribution manifold (14) with a bottom parallel with the wall perpendicular to the gas distribution manifold (14) of the end fixed on the wall portions or wall; aeration is provided with means (15) on the gas distribution manifold (14).
2、根据权利要求1所述的曝气式光生物反应器,其特征在于所述的曝气装置(15)为微孔曝气管或穿孔曝气管或橡胶膜片微孔曝气器或钟罩式刚玉微孔曝气器或微孔圆盘曝气器或微孔圆盔曝气器或长条板形曝气器或双螺旋曝气器或旋混式曝气器或散流式曝气器或曝气软管或可变微孔曝气器或OXAZUR空气扩散器或单孔膜空气扩散器。 2. The aeration photobioreactor according to claim 1, wherein said aeration means (15) is a porous or perforated aeration tube aeration tubes or rubber diaphragm or porous aerator bell corundum porous or microporous aerator disk aerators circular helmet or microporous diffusers or aerators elongated plate-shaped or twin screw mixing aerator or aeration or spin diffuser formula aerator or aeration hoses or variable porous diffusers or diffuser or an air hole OXAZUR membrane air diffuser.
3、 根据权利要求1所述的曝气式光生物反应器,其特征在于所述的农用薄膜(6)采用有利于光合作用的无滴薄膜或转光膜。 3. The aeration photobioreactor according to claim 1, wherein said agricultural film (6) The no-drip facilitate photosynthesis film or film transfer.
4、 根据权利要求1所述的曝气式光生物反应器,其特征在于所述的透光盖板(8)采用钢化玻璃板或有机玻璃板或聚碳酸酯板或覆有农膜的扁钢框。 4. The aeration photobioreactor according to claim 1, wherein said light-transmitting cover (8) using the glass plate or organic glass plate or a polycarbonate plate or covered with a flat plastic sheeting steel frame.
5、 根据权利要求1所述的曝气式光生物反应器,其特征在于所述的热水盘管为无规共聚聚丙烯PP-R管或交联聚乙烯PE-X管或耐高温聚乙烯PE-RT管或铝塑复合管XPAP或耐冲击共聚聚丙烯PP-C(B)管或聚丁烯PB管或铜管。 5. The aeration photobioreactor according to claim 1, wherein said coil is a hot water PP-R polypropylene random copolymer or cross-linked polyethylene tube PE-X or refractory tube poly ethylene PE-RT pipes or plastic composite pipe XPAP or impact polypropylene copolymer PP-C (B) or polybutylene pipe PB pipe or brass.
6、 一种曝气式光生物反应器的应用方法,其特征在于进气总管(13) 接过滤空气与二氧化碳钢瓶气或二氧化碳工业废气的混合气,依靠曝气实现微藻培养中的气体交换;曝气装置曝气的气流方向与垂直向上方向的夹角为90〜160度之间,曝气产生的微小气泡与周围藻液的密度差,促使气泡上浮以及藻液下降,由此环状浅水池内产生无数个气液上下循环流动,使上下层藻液混合,而气泡的上浮强化了藻液中溶氧的解吸,氧气被气泡带至液面而释放,并且二氧化碳气体经曝气后也同时溶解在藻液中;当曝气式光生物反应器中积累的氧气浓度达到一定水平时,升高或平移透光盖板(8)用于通风及散热,同时加大反应器进气总管的进气量,加快排出氧气的速度,同时保证反应器中微小的正压;在光生物反应器中营养盐浓度下降后,通过环状补液管(9)及雾化喷嘴 6, an application method of an optical aeration bioreactor, characterized in that the intake manifold (13) connected to the filtered air with carbon dioxide or a mixed gas of carbon dioxide gas cylinder industrial gas, relying on aeration to achieve gas exchange in the culture microalgae ; aeration means the aeration air flow direction an angle to the vertical upward direction is between 90~160 degrees, the density of fine bubbles and the surrounding liquid algae aeration difference produced, and causes the bubbles floating algae was decreased, whereby the cyclic generating numerous shallow pool vertically circulating liquid, mixing the lower algae solution, and floating bubbles reinforced alginate dissolved oxygen desorption liquid, with bubbles of oxygen is released to the liquid surface, and also carbon dioxide gas after aeration while alginate dissolved in the liquid; when aeration photobioreactor accumulated oxygen concentration reaches a certain level, elevated or translating cover lens (8) for ventilation and cooling, the reactor while increasing the intake manifold intake air amount, the discharge speed of the oxygen gas, while maintaining a slight positive pressure in the reactor; the decrease in nutrient concentration in the photobioreactor, through an annular fluid tube (9) and an atomizing nozzle (10),均匀地向光生物反应器中补加营养液;在光生物反应器中由于通入大量二氧化碳导致培养液的pH值下降后,通过环状补液管(9)及雾化喷嘴(10),均匀地喷洒碱性溶液来提高培养液的pH值;在光生物反应器中培养液温度下降至微藻适宜生长的温度下限后,在热水盘管中通入热水对培养液进行加热及保温。 (10), uniformly to the photobioreactor supplemented with nutrient solution; in the photobioreactor due quantities of carbon dioxide into the culture medium results in reduction of the pH, through an annular fluid tube (9) and atomizing nozzle ( 10), uniformly spraying an alkaline solution to raise the pH of the culture medium; culture solution after the temperature dropped to a lower limit temperature suitable for the growth of algae in the photobioreactor, the hot water coil in the hot water into the culture solution heating and insulation.
7、根据权利要求6所述的曝气式光生物反应器的应用方法,其特征在于所述的碱性溶液为低浓度的氢氧化钠溶液、氢氧化钾溶液,或者高浓度的碳酸钠溶液、碳酸钾溶液。 7. The method of aeration applications 6 photobioreactor as claimed in claim, wherein said alkaline solution is a low concentration of sodium hydroxide solution, potassium hydroxide solution, or a high concentration solution of sodium carbonate , potassium carbonate solution.
CN 200910047898 2009-03-20 2009-03-20 Aeration type photobioreactor and method of use thereof CN101497473A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 200910047898 CN101497473A (en) 2009-03-20 2009-03-20 Aeration type photobioreactor and method of use thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 200910047898 CN101497473A (en) 2009-03-20 2009-03-20 Aeration type photobioreactor and method of use thereof

Publications (1)

Publication Number Publication Date
CN101497473A true CN101497473A (en) 2009-08-05

Family

ID=40944777

Family Applications (1)

Application Number Title Priority Date Filing Date
CN 200910047898 CN101497473A (en) 2009-03-20 2009-03-20 Aeration type photobioreactor and method of use thereof

Country Status (1)

Country Link
CN (1) CN101497473A (en)

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101914430A (en) * 2010-07-12 2010-12-15 张建洲 Device and method for cultivating microalgae
CN102172473A (en) * 2010-12-13 2011-09-07 李京陆 Biomass high-temperature smoke treatment method and system
CN102408983A (en) * 2010-09-21 2012-04-11 新奥科技发展有限公司 Bag-like photo-bioreactor
CN102816700A (en) * 2012-08-15 2012-12-12 三峡大学 Over-water enclosure type floating island algae cultivation technology
CN103096708A (en) * 2010-05-20 2013-05-08 通用原子公司 Microalgae growth pond design
CN103359877A (en) * 2013-07-12 2013-10-23 沈阳航空航天大学 High-concentration sewage multi-stage diatom ecological treatment system
CN103361137A (en) * 2013-07-12 2013-10-23 沈阳航空航天大学 Closed methane water body ecological cleaning system
CN103848498A (en) * 2014-04-02 2014-06-11 苏州市玄天环保科技有限公司 Atomizing aeration tank
CN104762212A (en) * 2015-04-16 2015-07-08 青岛华盛绿能农业科技有限公司 Method for culturing haematococcus pluvialis by photovoltaic greenhouse
CN106281970A (en) * 2016-09-22 2017-01-04 东莞市联洲知识产权运营管理有限公司 A kind of open microdisk electrode pond
CN106675992A (en) * 2017-01-17 2017-05-17 杭州元点生物科技有限公司 Circular stirring system capable of controlling microalgae culture
TWI586607B (en) * 2016-07-06 2017-06-11 顥鎮企業有限公司 A water distribution device
CN107905566A (en) * 2017-12-06 2018-04-13 山西农业大学 Microalgae green energy conservation intelligent bus station

Cited By (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103096708A (en) * 2010-05-20 2013-05-08 通用原子公司 Microalgae growth pond design
CN101914430A (en) * 2010-07-12 2010-12-15 张建洲 Device and method for cultivating microalgae
CN101914430B (en) * 2010-07-12 2013-04-10 张建洲 Device and method for cultivating microalgae
CN102408983A (en) * 2010-09-21 2012-04-11 新奥科技发展有限公司 Bag-like photo-bioreactor
CN102408983B (en) * 2010-09-21 2014-08-13 新奥科技发展有限公司 Bag-like photo-bioreactor
CN102172473B (en) * 2010-12-13 2013-05-29 李京陆 Biomass high-temperature smoke treatment method and system
CN102172473A (en) * 2010-12-13 2011-09-07 李京陆 Biomass high-temperature smoke treatment method and system
CN102816700A (en) * 2012-08-15 2012-12-12 三峡大学 Over-water enclosure type floating island algae cultivation technology
CN102816700B (en) * 2012-08-15 2014-09-10 三峡大学 Over-water enclosure type floating island algae cultivation technology
CN103361137B (en) * 2013-07-12 2015-04-22 沈阳航空航天大学 Closed methane water body ecological cleaning system
CN103359877A (en) * 2013-07-12 2013-10-23 沈阳航空航天大学 High-concentration sewage multi-stage diatom ecological treatment system
CN103361137A (en) * 2013-07-12 2013-10-23 沈阳航空航天大学 Closed methane water body ecological cleaning system
CN103848498A (en) * 2014-04-02 2014-06-11 苏州市玄天环保科技有限公司 Atomizing aeration tank
CN104762212A (en) * 2015-04-16 2015-07-08 青岛华盛绿能农业科技有限公司 Method for culturing haematococcus pluvialis by photovoltaic greenhouse
TWI586607B (en) * 2016-07-06 2017-06-11 顥鎮企業有限公司 A water distribution device
CN106281970A (en) * 2016-09-22 2017-01-04 东莞市联洲知识产权运营管理有限公司 A kind of open microdisk electrode pond
CN106675992A (en) * 2017-01-17 2017-05-17 杭州元点生物科技有限公司 Circular stirring system capable of controlling microalgae culture
CN107905566A (en) * 2017-12-06 2018-04-13 山西农业大学 Microalgae green energy conservation intelligent bus station

Similar Documents

Publication Publication Date Title
US7056725B1 (en) Vegetable alga and microbe photosynthetic reaction system and method for the same
CN201176435Y (en) Two-row strong rotating solar methane tank
US8241634B2 (en) Carbon supply device for cultivating micro-algae in large scale and its application method and use
US8877486B2 (en) Photobioreactor for carbon dioxide mitigation in wastewater treatment
de Ortega et al. Production of Chlorella biomass in different types of flat bioreactors in temperate zones
CN100587054C (en) Novel multi-section flat type photo-bioreactor
CN101575567A (en) Method for culturing microalgae by illumination way and reactor thereof
CN1317379C (en) Pipeline photobioreactor for scale culture of microalgae
CN1201826A (en) Spirulina light illuminating tower-tray optic bio-reactor culturing system and its controlling method
CN101870950B (en) Device for culturing microalgae
JP3111873U (en) Plant algae / microorganism photosynthesis reactor
JP3111873U6 (en) Plant algae / microorganism photosynthesis reactor
CN102443535B (en) Solid fermentation bioreactor
CN1475558A (en) Photosynthesis bioreactor system for industrial production of micro algae
CN2234443Y (en) Spirulina photosynthesis reactor
CN101597567B (en) Photobioreactor
Tredici et al. Cultivation of Spirulina (Arthrospira) platensis in flat plate reactors
US9260685B2 (en) System and plant for cultivation of aquatic organisms
CN101555455B (en) Culture/harvesting method of microalgae and system
CN1064403C (en) Miniature aglae producing system of pipe air lift magnetic treatment optical biological reactor and monitoring method
CN101659922B (en) Closed raceway pond microalgae culture system
CN101735948B (en) Method and device for cultivating microalgae
Grima et al. Biomass and icosapentaenoic acid productivities from an outdoor batch culture of Phaeodactylum tricornutum UTEX 640 in an airlift tubular photobioreactor
CN101280271A (en) Production unit for microalgae industrialization and method for producing microalgae
CN101050419B (en) Production apparatus for culturing tiny alga and production method

Legal Events

Date Code Title Description
C06 Publication
C10 Request of examination as to substance
C12 Rejection of an application for a patent