CN101497473A - Aeration type photobioreactor and method of use thereof - Google Patents

Aeration type photobioreactor and method of use thereof Download PDF

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Publication number
CN101497473A
CN101497473A CNA2009100478987A CN200910047898A CN101497473A CN 101497473 A CN101497473 A CN 101497473A CN A2009100478987 A CNA2009100478987 A CN A2009100478987A CN 200910047898 A CN200910047898 A CN 200910047898A CN 101497473 A CN101497473 A CN 101497473A
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pipe
type
aeration
bioreactor
ring
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蔡志武
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SHANGHAI DAQI ENVIRONMENTAL PROTECTION ENGINEERING Co Ltd
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SHANGHAI DAQI ENVIRONMENTAL PROTECTION ENGINEERING Co Ltd
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
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Abstract

The invention relates to the field of microalgae cultivation engineering, in particular to an aeration type optical bioreactor for cultivating microalgae and a method for applying the same. The bioreactor comprises an annular wading pool, a liquid inlet main pipe, an annular liquid replenishing pipe, an atomization nozzle, a hot water pipe coil, an air inlet main pipe, an annular air inlet trunk pipe, an air distribution branch pipe and an aeration device. The bioreactor is characterized in that density difference between microbubbles generated by the aeration device through aeration and peripheral algae liquid promotes the floating of the microbubbles and the declining of the algae liquid; countless gas liquid generated by the annular wading pool circulates and flows up and down so that the upper layer and the lower layer of the algae liquid are mixed; the floating of the bubbles strengthens desorption of dissolved oxygen in the algae liquid; oxygen is taken to the liquid level by the bubbles and is released; and carbon dioxide gas is aerated and simultaneously dissolved in the algae liquid to provide a carbon source for the growth of the microalgae. The invention combines the advantages of an open type optical bioreactor and a closed type optical bioreactor to form a novel closed type optical bioreactor which can use the carbon dioxide gas as the carbon source and cultivate the microalgae in low cost and large scale.

Description

Aeration type bioreactor and application method thereof
[technical field]
The present invention relates to little algae culturing engineering field, specifically a kind of aeration type bioreactor and application method thereof of cultivating little algae.
[background technology]
Little algae can effectively utilize luminous energy, carbonic acid gas and inorganic salt, the biologically active substance of synthetic fat, protein, polysaccharide and multiple high added value.Along with the continuous intensification of the mankind to little algae understanding, develop and develop new and effective bioreactor and cultural method thereof, become an important component part of little algae biotechnology.
At present, little algae is cultivated and mainly contains open and closed two kinds of bioreactors.Open bioreactor mainly contains four types: on-swimmer's pool, circulatory pool, runway pond, pond.Wherein, the most typical, the most the most frequently used open pond culture systems is the runway pond reactor of Oswald (1969) design, and in fact such culture systems is exactly thousands of square metres of floor spaces, and the nutrient solution degree of depth is the shallow annular pond about 20 centimetres; With the natural light is light source and thermal source, relies on the rotation of paddle wheel (paddle wheel) or pivot arm (rotating arm), makes nutrient solution mix in the pond, circulate, and prevents the efficiency of light energy utilization of frond precipitation and raising frond cell.Though it is simple, with low cost, easy and simple to handle that open bioreactor makes up, there are shortcomings such as vulnerable to pollution, culture condition instability in it, can only be used for little algae cultivation that minorities such as spirulina, chlorella and salt algae can tolerate extreme environment.And the closed reactor culture condition is stable, but high-density culture is easily carried out in aseptic technique, has become developing direction from now on.Compare with open bioreactor, closed photo bioreactor has the following advantages: 1. pollution-free, can realize single kind, purebred cultivation; 2. culture condition is easy to control; 3. the culture density height is easily gathered in the crops; 4. the light autotrophy that is suitable for all little algaes is cultivated, and is particularly suitable for the production of little algae meta-bolites; 5. the ratio of higher illuminating area and volume of culture is arranged, and luminous energy and carbonic acid gas utilization ratio are higher.Therefore, development of closed photo bioreactor at home and abroad in recent years and development and use are very fast, and some has realized the high-density commercialization cultivation of little algae.At present closed photo bioreactor has: duct type, flat, column air lift type, stirred-tank fermenter, vertical Pig, floating bag film etc.
Existing closed photo bioreactor causes easily more or less that dissolved oxygen is accumulated, variety of issues such as under-supply, the flow blind angle of carbonic acid gas, equipment manufacturing cost costliness when the sticking wall of frond, high-density culture, except pipeline reactor, substantially all be in laboratory scale, volume is no more than 1 cubic meter.Though and the annual output that the duct type bioreactor is reported has at present reached up to a hundred tons of algae powder, the cost of this equipment and maintenance cost are very high, are difficult to popularize.
In fact, no matter which kind of design form bioreactor adopts, and develops the closed photo bioreactor of a kind of successful little algae of cultivation, all should follow following principle: (1) high illumination surface to volume ratio; (2) higher gas exchange efficiency; (3) Recycle design of Shi Heing; (4) improve route of transmission, distribution and the quality of light; (5) prevent the accumulation of harmful secondary metabolite; (6) more easily realize the optimal control of culture condition; (7) reduce manufacturing cost as far as possible.Can predict, the sort of cost is low, volume big, the more rational closed photo bioreactor of design should be the emphasis and the developing direction of exploitation.
What the carbon source that little algae is supplied with in culturing process was the most frequently used is sodium bicarbonate, this cost accounts for 40~70% of raw materials cost, if with the carbonic acid gas is that carbon source replaces sodium bicarbonate, its utilization of carbon source rate height, low price, can reduce cost significantly, the cost of carbon source can reduce about 90% in theory.Be that the bioreactor of carbon source must guarantee that carbon dioxide is disperseed well and absorbs with the carbon dioxide, and the oxygen that little algae produces is by desorb effectively, and the uniform and well blend of nutrient solution.
[summary of the invention]
The objective of the invention is to overcome defective and the deficiency that above-mentioned present technology exists, develop a kind of less investment, simple to operate, can make full use of solar energy, structure of reactor is succinct, practical, and can be carbon source with the carbon dioxide, carry out the aeration type bioreactor of large-scale culturing micro-algae.
The aeration type bioreactor comprises ring-type on-swimmer's pool 1, divider wall 2, liquid discharge pipe 3, blow-off pipe 4, band steel 5, agricultural film 6, skylight 7, euphotic cover plate 8, ring-type liquid supplementation pipe 9, atomizing nozzle 10, feed liquor house steward 11, ring-type air inlet main 12, the inlet manifold 13, gas distributionbranch pipe 14, aerating apparatus 15, hot-water coil pipe, it is characterized in that: reactor is a ring-type on-swimmer's pool 1, ring-type on-swimmer's pool middle part is provided with the divider wall 2 of one both ends open, aeration type bioreactor right part lower end is provided with liquid discharge pipe 3, the bottom of reactor is provided with blow-off pipe 4, aeration type bioreactor top is provided with passive band steel 5, the two ends of band steel and pool wall upper end are connected and fixed, band steel 5 is provided with agricultural film 6 and covers whole ring-type on-swimmer's pool 1, become closed photo bioreactor, be provided with skylight 7 on agricultural film 6, the skylight is provided with euphotic cover plate 8; Be provided with the ring-type liquid supplementation pipe 9 around divider wall 2 below the band steel 5, the bottom of liquid supplementation pipe is provided with atomizing nozzle 10, and ring-type liquid supplementation pipe 9 is connected with feed liquor house steward 11; The sidewall of the bottom of ring-type on-swimmer's pool 1, sidewall and divider wall 2 is provided with hot-water coil pipe; The middle and lower part of ring-type on-swimmer's pool 1 is provided with the parallel ring-type air inlet main 12 around divider wall 2, ring-type air inlet main is connected with inlet manifold 13, be connected with gas distributionbranch pipe 14 on the ring-type air inlet main 12, gas distributionbranch pipe is with parallel at the bottom of the pond, and vertical with pool wall, the end of gas distributionbranch pipe 14 is separately fixed on pool wall or the divider wall; Gas distributionbranch pipe 14 is provided with aerating apparatus 15.
Described aerating apparatus 15 is micropore aeration pipe or boring aeration pipe or rubber diaphragm micro-hole aerator or bell-jar corundum microporous aerator device or micropore disk aerator or micropore circle helmet aerator or rectangular plate shape aerator or duplex aerator or rotary mixing type aerator or turbulent flow type aerator or aeration flexible pipe or variable micro-hole aerator or OXAZUR air diffuser or single hole film air diffuser.
Described euphotic cover plate 8 adopts armourplate glass or poly (methyl methacrylate) plate or polycarbonate plate or is covered with the band steel frame of plastic sheeting for farm use.
Described hot-water coil pipe is atactic copolymerized polypropene PP-R pipe or crosslinked polyethylene PE-X pipe or refractory polyethylene PE-RT pipe or aluminum-plastic laminated tube XPAP or shock-resistant Co-polypropylene PP-C (B) pipe or polybutene PB pipe or copper pipe.
The application method of aeration type bioreactor is characterized in that inlet manifold 13 takes over the gas mixture of air filtering and carbon dioxide steel cylinder gas or carbonic acid gas industrial gaseous waste, and the dependence aeration is realized the gaseous interchange in little algae cultivation; The air flow line of aerating apparatus aeration and vertically upward the angle of direction be 90~160 the degree between, the micro-bubble that aeration produces and the density difference of algae liquid on every side, impel bubble floating and algae liquid to descend, producing numerous gas-liquid thus in the ring-type on-swimmer's pool circulates up and down, levels algae liquid is mixed, be that the gas-liquid density difference causes convection current to mix, and the come-up of bubble has been strengthened the desorb of dissolved oxygen in the algae liquid, oxygen is brought to liquid level by bubble and discharges, and carbon dioxide also is dissolved in behind aeration in the algae liquid simultaneously, for the growth of little algae provides carbon source; When the oxygen concentration that accumulates in the aeration type bioreactor reaches certain level, rising or translation light-passing board ventilate and dispel the heat, strengthen reactor inlet manifold's air input simultaneously, be used to accelerate to discharge the speed of oxygen, guarantee malleation small in the reactor simultaneously, prevent that microorganism and the dust in the outside air from entering reactor; After nutrient concentration descends in bioreactor,, in bioreactor, add nutritive medium equably by ring-type liquid supplementation pipe 9 and atomizing nozzle 10; In bioreactor,,, spray the pH value that basic solution improves nutrient solution equably by ring-type liquid supplementation pipe 9 and atomizing nozzle 10 owing to feed after great amount of carbon dioxide causes the pH value decline of nutrient solution; After culture-liquid temp dropped to the lowest temperature of little algae suitable growth in bioreactor, feeding hot water heated nutrient solution and is incubated in hot-water coil pipe.
Described CO 2 waste gas is the stack gas from power plant, Steel Plant, cement mill, ceramics factory, chemical plant, and the carbon dioxide process waste gas of biological fermentation factory.
Described little algae is freshwater microalgae or little algae of salty lake water or the little algae of seawater, and classification is chrysophyceae or green alga or diatom or blue-green algae or red algae, is preferably spirulina or grape algae or chlorella or Isochrysis galbana 3011 or pavlova viridis.The basic solution of described sprinkling is sodium hydroxide solution, the potassium hydroxide solution of lower concentration, the perhaps sodium carbonate solution of high density, solution of potassium carbonate.
Rely on aeration to realize the gaseous interchange of little algae in cultivating because the present invention cultivates the production equipment of little algae, saved huge paddle wheel or pivot arm, whole reactor can intactly cover with agricultural film 6 easily.The agricultural film is here selected for use and is helped photosynthetic film, for example can prevent from film to form droplet and the fogging-resistance film that reduces transmittance, and utilize optical principle to convert to light to the favourable light-converting film of little algae photosynthesis etc.The skylight is left at middle part at agricultural film 6, is stamped the euphotic cover plate 8 of band lifting or translating device on the skylight.In the summer of sweltering heat, can on agricultural film 6, cover one deck photomask again, prevent that illumination from crossing strong and suppress even damage the growth of little algae, reduce reactor temperature simultaneously.
This aeration type bioreactor is provided with pH electrode, dissolved oxygen electrode, temperature sensor, liquidometer, by transmitter signal is delivered in the computer to show.Simultaneously, all be provided with on the inlet manifold of reactor, feed liquor house steward, the liquid discharge pipe and manually reach automatic valve, euphotic cover plate on the reactor skylight also is provided with manually and raises automatically or translating device, after computer is handled input signal, according to the culture process of little algae, can regulate the aperture in each valve and skylight automatically.In addition, the detection of algae liquid concentration both can also can be installed the OD transmitter, automatically continuous detecting OD value from the skylight opening of reactor to its OD value of algae liquid sampling analysis in the algae liquid of inside reactor.
The present invention is in conjunction with open and closed photo bioreactor advantage separately, forming a kind of can be carbon source with the carbon dioxide, the novel closed-type bioreactor of low cost, large-scale culturing micro-algae, this will play a significant role in the industrialization process that advances both culturing microalgae.
[description of drawings]
Fig. 1 cultivates the structural representation of the aeration type bioreactor of little algae for the present invention.
Referring to Fig. 1,1 is the ring-type on-swimmer's pool, and 2 is divider wall, 3 is liquid discharge pipe, and 4 is blow-off pipe, and 5 is band steel, 6 is agricultural film, and 7 is the skylight, and 8 is euphotic cover plate, 9 is the ring-type liquid supplementation pipe, and 10 is atomizing nozzle, and 11 is the feed liquor house steward, 12 is ring-type air inlet main, 13 is the inlet manifold, and 14 is gas distributionbranch pipe, and 15 is aerating apparatus.
[embodiment]
The invention will be further described below in conjunction with drawings and Examples.Manufacturing technology of the present invention is to implement easily to this professional people.
As Fig. 1, the aeration type bioreactor of cultivating Isochrysis galbana 3011 is a ring-type on-swimmer's pool 1, and enclosure wall is built by laying bricks or stones by fragment of brick, and the surface is floating with cement, then lays composite geo-membrane at the bottom of the pond of ring-type on-swimmer's pool 1, in the pond with the divider wall 2 of brick one both ends open.The width in pond is 4 meters, and length is 100 meters, and the degree of depth is 60 centimetres, and divider wall 2 highly is 70 centimetres.Aeration type bioreactor right part lower end is provided with liquid discharge pipe 3, and the bottom is provided with blow-off pipe 4.Aeration type bioreactor top is provided with an arch band steel 5 that queues supporting role, and the two ends of band steel are fixed on the pool wall upper end.Adopt agricultural film 6 intactly to cover whole ring-type on-swimmer's pool 1 on the band steel, become closed photo bioreactor, be provided with skylight 7, be stamped euphotic cover plate 8 on the skylight at the middle part of agricultural film 6.Be fixed with the ring-type liquid supplementation pipe 9 around divider wall 2 below the band steel 5, the bottom of liquid supplementation pipe has many atomizing nozzles 10, and ring-type liquid supplementation pipe 9 is connected with feed liquor house steward 11.Be covered with hot-water coil pipe on the sidewall of the bottom of ring-type on-swimmer's pool 1, sidewall and divider wall 2, tubing is preferably the PP-R pipe.The middle and lower part of ring-type on-swimmer's pool 1 is fixed with 2 parallel ring-type air inlet mains 12 around divider wall 2, and ring-type air inlet main all is connected with inlet manifold 13, and inlet manifold 13 takes over the gas mixture of the carbonic acid gas stack gas of air filtering and near heat power plant.Be connected with several gas distributionbranch pipes 14 on the ring-type air inlet main 12, gas distributionbranch pipe is with parallel at the bottom of the pond, and vertical with pool wall, and the end of gas distributionbranch pipe is separately fixed on pool wall or the divider wall; Gas distributionbranch pipe 14 is provided with a plurality of silicon rubber micropore aeration pipes 15, and the air flow line of this tube aerator aeration is level forward.The come-up bubble that aeration produces mixes levels algae liquid, and the come-up of bubble has been strengthened the desorb of dissolved oxygen in the algae liquid, oxygen is brought to liquid level by bubble and discharges, and carbon dioxide also is dissolved in behind aeration in the algae liquid simultaneously, for the growth of little algae provides carbon source.After nutrient concentration descends in bioreactor,, in bioreactor, add nutritive medium equably by ring-type liquid supplementation pipe 9 and atomizing nozzle 10; Owing to feed after great amount of carbon dioxide causes the pH value decline of nutrient solution, by ring-type liquid supplementation pipe 9 and atomizing nozzle 10, the sodium hydroxide solution that sprays lower concentration equably improves the pH value of nutrient solution in bioreactor.After culture-liquid temp drops to 15 ℃ in bioreactor, in hot-water coil pipe, feed 50 ℃ of hot water nutrient solution is heated to 25 ℃ and insulation.
Whole aeration type bioreactor intactly covers with agricultural film 6, forms closed photo bioreactor.The plastic sheeting for farm use is here selected long-life polyvinyl chloride fogging-resistance film for use, prevents from the film to form droplet and reduces transmittance.Middle part at film has the skylight, is stamped the polycarbonate euphotic cover plate 8 of band lifting device on the skylight, and when the oxygen concentration that accumulates in the aeration type bioreactor reached certain level, the rising light-passing board ventilated and dispels the heat.In the summer of sweltering heat, can on agricultural film 6, cover one deck photomask again, prevent that illumination from crossing strong and suppress even damage the growth of little algae, reduce reactor temperature simultaneously.
PH electrode, dissolved oxygen electrode, temperature sensor, liquidometer etc. are housed in the aeration type bioreactor, by transmitter signal are delivered in the computer and shown.Simultaneously, on the inlet manifold of reactor, feed liquor house steward, the liquid discharge pipe hand sluice valve is housed all, the detection of algae liquid concentration can be from the skylight opening of reactor to its OD value of algae liquid sampling analysis.

Claims (7)

1, a kind of aeration type bioreactor comprises ring-type on-swimmer's pool (1), divider wall (2), liquid discharge pipe (3), blow-off pipe (4), band steel (5), agricultural film (6), skylight (7), euphotic cover plate (8), ring-type liquid supplementation pipe (9), atomizing nozzle (10), feed liquor house steward (11), ring-type air inlet main (12), inlet manifold (13), gas distributionbranch pipe (14), aerating apparatus (15), hot-water coil pipe, it is characterized in that: reactor is a ring-type on-swimmer's pool (1), ring-type on-swimmer's pool (1) middle part is provided with the divider wall (2) of one both ends open, aeration type bioreactor right part lower end is provided with liquid discharge pipe (3), the bottom of reactor is provided with blow-off pipe (4), aeration type bioreactor top is provided with band steel (5), the two ends of band steel are connected with the pool wall upper end, band steel (5) is provided with the agricultural film (6) that covers whole ring-type on-swimmer's pool (1), be provided with skylight (7) on agricultural film (6), skylight (7) are provided with euphotic cover plate (8); Be provided with the ring-type liquid supplementation pipe (9) around divider wall (2) below the band steel (5), the bottom of liquid supplementation pipe is provided with atomizing nozzle (10), and ring-type liquid supplementation pipe (9) is connected with feed liquor house steward (11); The sidewall of bottom, sidewall and the divider wall (2) of ring-type on-swimmer's pool (1) is provided with hot-water coil pipe; The middle and lower part of ring-type on-swimmer's pool (1) is provided with the parallel ring-type air inlet main (12) around divider wall (2), ring-type air inlet main (12) is connected with inlet manifold (13), ring-type air inlet main (12) is gone up and is connected with gas distributionbranch pipe (14), gas distributionbranch pipe (14) is with parallel at the bottom of the pond, and vertical with pool wall, the end of gas distributionbranch pipe (14) is separately fixed on pool wall or the divider wall; Gas distributionbranch pipe (14) is provided with aerating apparatus (15).
2, aeration type bioreactor according to claim 1 is characterized in that described aerating apparatus (15) is micropore aeration pipe or boring aeration pipe or rubber diaphragm micro-hole aerator or bell-jar corundum microporous aerator device or micropore disk aerator or micropore circle helmet aerator or rectangular plate shape aerator or duplex aerator or rotary mixing type aerator or turbulent flow type aerator or aeration flexible pipe or variable micro-hole aerator or OXAZUR air diffuser or single hole film air diffuser.
3, aeration type bioreactor according to claim 1 is characterized in that described agricultural film (6) employing helps photosynthetic fogging-resistance film or light-converting film.
4, aeration type bioreactor according to claim 1 is characterized in that described euphotic cover plate (8) adopts armourplate glass or poly (methyl methacrylate) plate or polycarbonate plate or is covered with the band steel frame of plastic sheeting for farm use.
5, aeration type bioreactor according to claim 1 is characterized in that described hot-water coil pipe is atactic copolymerized polypropene PP-R pipe or crosslinked polyethylene PE-X pipe or refractory polyethylene PE-RT pipe or aluminum-plastic laminated tube XPAP or shock-resistant Co-polypropylene PP-C (B) pipe or polybutene PB pipe or copper pipe.
6, a kind of application method of aeration type bioreactor is characterized in that inlet manifold (13) takes over the gas mixture of air filtering and carbon dioxide steel cylinder gas or carbonic acid gas industrial gaseous waste, and the dependence aeration is realized the gaseous interchange in little algae cultivation; The air flow line of aerating apparatus aeration and vertically upward the angle of direction be 90~160 the degree between, the micro-bubble that aeration produces and the density difference of algae liquid on every side, impel bubble floating and algae liquid to descend, producing numerous gas-liquid thus in the ring-type on-swimmer's pool circulates up and down, levels algae liquid is mixed, and the come-up of bubble has been strengthened the desorb of dissolved oxygen in the algae liquid, and oxygen is brought to liquid level by bubble and discharges, and carbon dioxide also is dissolved in the algae liquid behind aeration simultaneously; When the oxygen concentration that accumulates in the aeration type bioreactor reaches certain level, rising or translation euphotic cover plate (8) are used for ventilating and heat radiation, strengthen reactor inlet manifold's air input simultaneously, accelerate to discharge the speed of oxygen, guarantee malleation small in the reactor simultaneously; After nutrient concentration descends in bioreactor,, in bioreactor, add nutritive medium equably by ring-type liquid supplementation pipe (9) and atomizing nozzle (10); In bioreactor,,, spray the pH value that basic solution improves nutrient solution equably by ring-type liquid supplementation pipe (9) and atomizing nozzle (10) owing to feed after great amount of carbon dioxide causes the pH value decline of nutrient solution; After culture-liquid temp dropped to the lowest temperature of little algae suitable growth in bioreactor, feeding hot water heated nutrient solution and is incubated in hot-water coil pipe.
7, the application method of aeration type bioreactor according to claim 6 is characterized in that described basic solution is sodium hydroxide solution, the potassium hydroxide solution of lower concentration, the perhaps sodium carbonate solution of high density, solution of potassium carbonate.
CNA2009100478987A 2009-03-20 2009-03-20 Aeration type photobioreactor and method of use thereof Pending CN101497473A (en)

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CN101914430B (en) * 2010-07-12 2013-04-10 张建洲 Device and method for cultivating microalgae
CN101914430A (en) * 2010-07-12 2010-12-15 张建洲 Device and method for cultivating microalgae
CN102408983B (en) * 2010-09-21 2014-08-13 新奥科技发展有限公司 Bag-like photo-bioreactor
CN102408983A (en) * 2010-09-21 2012-04-11 新奥科技发展有限公司 Bag-like photo-bioreactor
CN102172473A (en) * 2010-12-13 2011-09-07 李京陆 Biomass high-temperature smoke treatment method and system
CN102172473B (en) * 2010-12-13 2013-05-29 李京陆 Biomass high-temperature smoke treatment method and system
CN102816700A (en) * 2012-08-15 2012-12-12 三峡大学 Over-water enclosure type floating island algae cultivation technology
CN102816700B (en) * 2012-08-15 2014-09-10 三峡大学 Over-water enclosure type floating island algae cultivation technology
CN103361137B (en) * 2013-07-12 2015-04-22 沈阳航空航天大学 Closed methane water body ecological cleaning system
CN103359877A (en) * 2013-07-12 2013-10-23 沈阳航空航天大学 High-concentration sewage multi-stage diatom ecological treatment system
CN103361137A (en) * 2013-07-12 2013-10-23 沈阳航空航天大学 Closed methane water body ecological cleaning system
CN103848498A (en) * 2014-04-02 2014-06-11 苏州市玄天环保科技有限公司 Atomizing aeration tank
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