CN111704990B - Tube-pool combined flash bioreactor system and microalgae growth carbon fixation method thereof - Google Patents
Tube-pool combined flash bioreactor system and microalgae growth carbon fixation method thereof Download PDFInfo
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Abstract
The invention relates to biomass energy utilization technology, and aims to provide a tube-pool combined flash bioreactor system and a microalgae growth carbon fixation method thereof. The system comprises a raceway pond reactor for culturing microalgae; a plurality of horizontal transverse pipes made of light-transmitting materials are arranged above the horizontal transverse pipes in parallel, and the horizontal transverse pipes are mutually perpendicular to the length direction of the runway pool reactor; the end parts of the adjacent horizontal transverse pipes are connected by bent pipes to form a through horizontal transverse pipe reactor; the outlet end of the horizontal tube reactor is sequentially connected with the runway pool reactor and the CO through pipelines 2 The outlet end of the dissolver is sequentially connected with the centrifugal pump and the inlet end of the horizontal cross tube reactor through a pipeline; in CO 2 The dissolver is provided with a gas distributor which is connected to the CO through a pipeline 2 And (5) an air source. The invention can improve the distribution of the light area and the dark area on the surface of the algae liquid in the runway pool, obviously strengthen the flash effect of the flowing microalgae cells, improve the flash frequency of the movement of the microalgae cells in the runway pool, and is beneficial to promoting the photosynthesis growth of the microalgae to fix carbon.
Description
Technical Field
The invention relates to biomass energy utilization technology, in particular to a tube-pool combined flash bioreactor system and a microalgae growth carbon fixation method thereof.
Background
The microalgae has high growth speed, high light energy utilization efficiency and high economic value of biomass, so the microalgae becomes flue gas CO 2 Leading edge research hotspots in the fields of emission reduction and new energy development. The microalgae is fully utilized to capture CO in the exhaust smoke of coal-fired power plants, coal chemical plants, industrial furnaces and kilns and the like 2 The biomass rich in high-value active ingredients can be obtained while the cost of microalgae production raw materials is reduced. The runway pool reactor is used as the most successful commercial cultivation mode of microalgae cultivation industry, and the equipment and the process are simple to operate and easy to amplify, so that the runway pool reactor is widely applied at home and abroad.
The penetration distance of solar rays in the algae liquid is very limited, and microalgae cells at the bottom of the runway pool are far away from the light receiving surface of the algae liquid, so that sufficient light energy is difficult to obtain for photosynthesis. However, when the sunlight of the external environment is too strong, the microalgae cells on the surface of the runway pool algae liquid are exposed to strong light for a long time, so that the light energy is forced to be received to exceed the utilization amount of a cell photosynthetic system, and the cell photosynthetic efficiency is reduced. Researchers define the region of the reactor where the light intensity is below a certain threshold as the dark region and vice versa. The research shows that microalgae need to be continuously and rapidly changed between a light area and a dark area in a reactor culture solution so as to obtain higher light energy utilization efficiency and cell growth speed. Therefore, the conversion movement times of the microalgae cells between the light area and the dark area in each second time are called as the flash frequency, and the flash frequency of the movement of the microalgae cells can be effectively improved by improving the internal structure of the reactor and optimizing the distribution characteristics of the light area and the dark area. The Yang et al set up vortex baffle in the race pool inside and produced vortex flow field to strengthen the flash frequency of little algae motion and improve growth rate, promote the gas-liquid mixing mass transfer through reactor structure optimization and make little algae biomass yield improve 22%. However, the turbulent flow baffle plate increases the flow resistance and reduces the flow speed of the algae liquid, so that a novel reactor structure is necessary to be developed to improve the flash frequency and promote the growth of microalgae to fix carbon.
Disclosure of Invention
The invention aims to solve the technical problem of overcoming the defects in the prior art and providing a tube-pool combined flash bioreactor system and a microalgae growth carbon fixation method thereof.
In order to solve the technical problems, the invention adopts the following solutions:
providing a tube-pool combined flash bioreactor system comprising a raceway pool reactor for cultivating microalgae; the method is characterized in that a plurality of horizontal transverse pipes made of light-transmitting materials are arranged above the runway pool reactor in parallel, and the horizontal transverse pipes are mutually perpendicular to the length direction of the runway pool reactor; the end parts of the adjacent horizontal transverse pipes are connected by bent pipes to form a through horizontal transverse pipe reactor; the outlet end of the horizontal tube reactor is sequentially connected with the runway pool reactor and the CO through pipelines 2 Dissolver, CO 2 The outlet end of the dissolver is sequentially connected with the centrifugal pump and the inlet end of the horizontal cross tube reactor through a pipeline; in CO 2 The dissolver is provided with a gas distributor which is connected to the CO through a pipeline 2 And (5) an air source.
In the invention, the horizontal transverse pipes are divided into 1-5 layers and are overlapped in the vertical direction, and the number of the transverse pipes in each layer is 10-1000 columns; the pipe wall spacing of two adjacent pipes in the vertical direction is 1-5 times of the pipe diameter, and the pipe wall spacing of two adjacent pipes in the horizontal direction is 1-10 times of the pipe diameter.
In the invention, the number of the runway pool reactors is 3-10; the runway pool reactors are arranged in a manner that the length directions are parallel to each other and are connected with each other end to end in sequence; each runway pool reactor is internally provided with a stirring paddle wheel and a membrane aerator.
In the invention, the stirring paddle wheel is a cross paddle wheel made of stainless steel; the membrane aerator is a rubber material product, the aperture is 0.8mm, and the porosity is 0.85; the membrane aerator is connected with CO through a pipeline 2 And (5) an air source.
In the invention, the pipe diameter of the horizontal transverse pipe is 5 cm, and the minimum distance between the bottom of the horizontal transverse pipe and the bottom of the runway pool is 2 meters.
In the invention, the horizontal transverse tube is a common glass tube, a toughened glass tube or a high polymer plastic tube with the light transmittance of more than 80 percent.
The invention further provides a method for realizing carbon fixation of microalgae growth by utilizing the tube-pond combined flash bioreactor system, which comprises the following steps:
(1) Inoculating microalgae culture solution into CO 2 The dissolver calculates the inoculation mass ratio of the microalgae culture solution to be 5-10% by using the whole water body of the system; starting a centrifugal pump to enable algae liquid to enter the horizontal tube reactor at the upper layer for photosynthesis, and then enter the runway pool reactor at the lower layer for continuous growth and carbon fixation;
(2) CO from industrial flue gas 2 Simultaneously feeding CO 2 Gas distributor in dissolver and diaphragm aerator in runway pool reactor, forming NaHCO by carbonation reaction 3 To increase CO 2 The utilization efficiency; controlling CO 2 The flow rate is controlled to be 0.02-0.1 vvm compared with the ventilation rate of the whole water body of the system;
(3) The algae liquid circularly flows between the horizontal tube reactor and the runway pool reactor, and incident light is absorbed and utilized by the algae liquid in the two reactors; because the algae liquid can generate turbulence and waves when flowing in the horizontal tube reactor, after the incident light rays penetrate through the horizontal tube reactor on the upper layer, a region with alternate brightness is formed on the surface of the runway pool reactor, so that a flashing effect is formed to promote the growth of microalgae cells; after 3-5 days of system operation, biomass is harvested.
In the invention, the ambient temperature of the whole system is controlled to be 10-38 ℃, and the illumination intensity of incident light on the upper surface of the horizontal tube reactor is 5000-100000Lux.
In the invention, the microalgae culture solution is prepared from microalgae such as nannochloropsis, chlorella or spirulina.
In the invention, the industrial flue gas CO 2 Refers to the exhaust gas of coal-fired power plants, the tail gas of coal chemical plants or the exhaust gas of industrial furnaces, wherein CO 2 The volume concentration is 10-99%.
Compared with the prior art, the invention has the beneficial effects that:
1. the tube-pool combined flash bioreactor system obviously improves the light area and dark area distribution on the surface of the algae liquid in the runway pool, obviously strengthens the flash effect of the flowing of microalgae cells, improves the flash frequency of the movement of the microalgae cells in the runway pool by 12.7-34.2%, and is beneficial to promoting the photosynthesis growth of the microalgae to fix carbon.
2. Because the pipe-pond combined type photoreactor comprises the special structures of the upper layer transverse pipe and the lower layer runway pond, the distribution of the projection area of incident light quanta in the reactor is improved, microalgae circulating in the reactor are prevented from being exposed to excessive light for a long time, and the light inhibition effect of the strong light on microalgae cells is effectively relieved. Therefore, the photochemical efficiency (Fv/Fm) of the optical system II (PS II) of the microalgae in the reactor is improved by 5.3 to 12.1 percent, the chlorophyll a content is improved by 11.6 to 21.1 percent, and the chlorophyll b content is improved by 6.3 to 11.2 percent, so that the microalgae can more efficiently convert light energy into ATP for synthesis of algae cytochrome and accumulation of biomass. Because the reactor system adopts a two-stage absorption mode for incident solar energy, and the upper horizontal transverse pipe and the lower runway pool are used for absorbing and utilizing the incident solar energy in sequence, the whole reactor system has high utilization rate of land resources and light energy, so that the growth rate of microalgae is improved by 21.5-36.9%, and the yield of microalgae biomass in unit occupied area is improved by 24.8-41.2%.
Drawings
FIG. 1 is a perspective view of a tube and tank combined flash bioreactor system according to the present invention.
Fig. 2 is a perspective view of a system from another perspective.
The reference numerals in the figures are: 1, a stirring paddle wheel; 2 a runway pool reactor; 3, a membrane aerator; 4CO 2 A dissolver; 5 a gas distributor; 6, a centrifugal pump; 7, a return pipe; 8, connecting pipes; 9 horizontal tube reactors.
Detailed Description
The invention is described in further detail below with reference to the drawings and the detailed description. The examples will allow a person skilled in the art to more fully understand the invention and are not intended to limit it in any way.
As shown in fig. 1, above the length direction of the raceway pond reactor 2, the horizontal transverse pipes are divided into 1-5 layers and are overlapped in the vertical direction (namely, the multi-layer transverse pipes are overlapped layer by layer in the vertical direction to form a row, so that gaps of the transverse pipes in the horizontal direction of each row are prevented from being blocked due to staggered arrangement), and the number of the transverse pipes in each layer is 10-1000 rows; the pipe wall spacing of two adjacent pipes in the vertical direction is 1-5 times of the pipe diameter, and the pipe wall spacing of two adjacent pipes in the horizontal direction is 1-10 times of the pipe diameter. 3-10 runway pool reactors 2 are arranged below the horizontal transverse pipe in the length direction, and the runway pool reactors 2 are arranged in a manner that the length directions are parallel to each other and are connected with each other end to end in sequence; each runway pool reactor 2 is internally provided with a stirring paddle wheel 1 and a membrane aerator 3. The end parts of the adjacent horizontal transverse pipes are connected by bent pipes to form a through horizontal transverse pipe reactor 9.
Therefore, the tube-pool combined flash bioreactor system is formed by combining an upper horizontal tube reactor 9 and a lower runway pool reactor 2, which are crossed. The outlet end of the horizontal tube reactor 9 is sequentially connected with the runway pool reactor 2 and CO through pipelines 2 Dissolver 4, CO 2 The outlet end of the dissolver 4 is sequentially connected with the centrifugal pump 6 and the inlet end of the horizontal cross tube reactor 9 through pipelines; in CO 2 A gas distributor 5 is arranged in the dissolver 4, and the gas distributor 5 is connected to the CO through a pipeline 2 And (5) an air source.
The stirring paddle wheel 1 is a cross paddle wheel made of stainless steel; the membrane aerator 3 is a rubber material product, the aperture is 0.8mm, and the porosity is 0.85; the membrane aerator 3 is connected with CO through a pipeline 2 And (5) an air source. The horizontal transverse tube can be a common glass tube, a toughened glass tube or a high polymer plastic tube with the light transmittance of more than 80 percent. The pipe diameter of the horizontal transverse pipe can be 5 cm, and the minimum distance between the bottom of the horizontal transverse pipe and the bottom of the runway pool can be 2 m.
The method for realizing carbon fixation of microalgae growth by utilizing the tube-pond combined flash bioreactor system comprises the following steps:
(1) Inoculating microalgae culture solution into CO 2 The dissolver calculates the inoculation mass ratio of the microalgae culture solution to be 5-10% by using the whole water body of the system; starting a centrifugal pump to enable algae liquid to enter the horizontal tube reactor at the upper layer for photosynthesis, and then enter the runway pool reactor at the lower layer for continuous growth and carbon fixation;
(2) CO from industrial flue gas 2 Simultaneously feeding CO 2 Gas distributor in dissolver and diaphragm aerator in runway pool reactor, forming NaHCO by carbonation reaction 3 To increase CO 2 The utilization efficiency; controlling CO 2 The flow rate is controlled to be 0.02-0.1 vvm compared with the ventilation rate of the whole water body of the system;
(3) The algae liquid circularly flows between the horizontal tube reactor and the runway pool reactor, and incident light is absorbed and utilized by the algae liquid in the two reactors; because the algae liquid can generate turbulence and waves when flowing in the horizontal tube reactor, after the incident light rays penetrate through the horizontal tube reactor on the upper layer, a region with alternate brightness is formed on the surface of the runway pool reactor, so that a flashing effect is formed to promote the growth of microalgae cells; after 3-5 days of system operation, biomass is harvested.
The microalgae culture solution can be selected from Chlorella, chlorella or Spirulina. Industrial flue gas CO 2 The flue gas discharged by a coal-fired power plant, the tail gas of a coal chemical plant or the flue gas discharged by an industrial furnace can be selected, wherein CO is contained in the flue gas 2 The volume concentration is 10-99%. The ambient temperature of the whole system is controlled to be 10-38 ℃, and the illumination intensity of incident light on the upper surface of the horizontal tube reactor is 5000-100000Lux.
Example 1
The tube-pool combined flash bioreactor system consists of upper horizontal tube and upper horizontal tubeThe lower layer runway pool is combined, and the horizontal cross pipe and the runway pool are crossed. 10 rows of horizontal transverse pipes are arranged above the length direction of the runway pool, and 3 runway pools are arranged below the length direction of the horizontal transverse pipes. The distance between the bottom of the horizontal transverse pipe and the bottom of the runway pool is 2 meters. The upper layer horizontal tube adopts a multilayer horizontal tube structure formed by vertically stacking, the tube diameters of the upper layer horizontal tube are all 5 cm, and the upper layer horizontal tube is made of light-transmitting tubes (namely high polymer tubes with light transmittance of more than 80%). The total number of layers of the horizontal transverse pipes is 1, and the pipe wall interval between two adjacent pipes in the horizontal direction is 10 times of the pipe diameter. The upper horizontal cross tube reactor equipment comprises CO 2 Dissolver, multi-group horizontal transverse pipe and algae liquid circulation system, wherein CO made of stainless steel material 2 The dissolver is a gas-liquid mixing device, and the internal gas distributor is connected to the CO through a pipeline 2 The device is characterized in that an algae liquid is inoculated to an inlet of a horizontal transverse tube; the algae liquid circulation system comprises a centrifugal pump and a connecting pipeline, which connects a horizontal pipe with CO 2 The dissolver is connected in a sealing way and is used for driving the algae liquid to be connected with CO in a horizontal transverse pipe 2 Circulation in the dissolver. The lower layer runway pool reactor equipment comprises a runway pool, a stirring paddle wheel and a gas supply system, wherein the stirring paddle wheel is a cross paddle wheel made of stainless steel; the gas supply system is a rubber membrane aerator and a connecting pipeline thereof, the aperture of the aerator is 0.8mm, and the porosity is 0.85. When the incident sunlight vertically irradiates the reactor system, the light is absorbed and utilized by the upper horizontal transverse pipe and the lower runway pool in sequence, and meanwhile, a region with alternate brightness is formed on the surface of the runway pool in the flowing direction of the algae liquid, so that a flash effect is formed when the microalgae cells in the runway pool flow, and the growth of the microalgae cells is promoted.
The method for realizing growth and carbon fixation of microalgae by utilizing a tube-pond combined flash bioreactor system comprises the following steps: inoculating microalgae culture solution (the microalgae species are nannochloropsis) into CO of a tube-pool combined flash bioreactor system 2 In the dissolver, the inoculation mass ratio of the microalgae culture solution to the water body in the whole tube-pond combined reactor is 5%. Introducing CO into the microalgae culture solution 2 After the dissolver is started, a centrifugal pump is started, algae liquid is pumped into a plurality of groups of horizontal tube reactors at the upper layer to carry out photosynthesis growth,then the plant is sent into a lower runway pool for photosynthesis growth. CO from industrial flue gas 2 (from flue gas discharged from coal-fired Power plant, CO) 2 10% by volume of CO 2 The gas distributor in the dissolver is sent into the upper horizontal cross pipe and simultaneously the industrial flue gas CO 2 Feeding the waste gas into a runway pool through a membrane aerator to control CO of industrial flue gas 2 The aeration rate of the flow compared to the water in the whole tube-pool combined reactor was 0.02vvm. The illumination intensity of the incident sunlight is 5000Lux, and the ambient temperature is 10 ℃. The algae liquid circularly flows between the upper horizontal transverse pipe and the lower runway pool, and biomass is harvested after photosynthesis growth is carried out for 3 days.
Compared with the traditional simple runway pool reactor (a horizontal transverse pipe is not arranged above the runway pool reactor), the pipe pool combined type flashing bioreactor system is adopted to improve the flashing frequency of the movement of microalgae cells by 12.7%, the photochemical efficiency (Fv/Fm) of a light system II (PS II) is improved by 5.3%, the chlorophyll a and chlorophyll b contents of the microalgae cells are respectively improved by 11.6% and 6.3%, the growth rate of the microalgae is improved by 21.5%, and the yield of the microalgae biomass of unit occupied area is improved by 24.8%.
Example 2
The tube-pool combined flash bioreactor system is formed by combining an upper horizontal cross tube and a lower runway pool, and the horizontal cross tube and the runway pool are crossed. 300 rows of horizontal transverse pipes are arranged above the length direction of the runway pool, and 6 runway pools are arranged below the length direction of the horizontal transverse pipes. The distance between the bottom of the horizontal transverse pipe and the bottom of the runway pool is 2 meters. The upper horizontal tube adopts a multi-layer horizontal tube structure formed by vertically stacking, the tube diameters of the upper horizontal tube are all 5 cm, and the upper horizontal tube is made of light-transmitting tubes (namely common glass with light transmittance of more than 80%). The total number of layers of the horizontal transverse pipes is 2, the pipe wall distance between two adjacent pipes in the vertical direction is 1 time of the pipe diameter, and the pipe wall distance between two adjacent pipes in the horizontal direction is 6 times of the pipe diameter. The upper horizontal cross tube reactor equipment comprises CO 2 Dissolver, multi-group horizontal transverse pipe and algae liquid circulation system, wherein CO made of stainless steel material 2 The dissolver is a gas-liquid mixing device, and the internal gas distributor is connected to the CO through a pipeline 2 A gas source, theThe device is to inoculate algae liquid to the inlet of the horizontal transverse tube; the algae liquid circulation system comprises a centrifugal pump and a connecting pipeline, which connects a horizontal pipe with CO 2 The dissolver is connected in a sealing way and is used for driving the algae liquid to be connected with CO in a horizontal transverse pipe 2 Circulation in the dissolver. The lower layer runway pool reactor equipment comprises a runway pool, a stirring paddle wheel and a gas supply system, wherein the stirring paddle wheel is a cross paddle wheel made of stainless steel; the gas supply system is a rubber membrane aerator and a connecting pipeline thereof, the aperture of the aerator is 0.8mm, and the porosity is 0.85. When the incident sunlight vertically irradiates the reactor system, the light is absorbed and utilized by the upper horizontal transverse pipe and the lower runway pool in sequence, and meanwhile, a region with alternate brightness is formed on the surface of the runway pool in the flowing direction of the algae liquid, so that a flash effect is formed when the microalgae cells in the runway pool flow, and the growth of the microalgae cells is promoted.
The method for realizing growth and carbon fixation of microalgae by utilizing a tube-pond combined flash bioreactor system comprises the following steps: inoculating microalgae culture solution (Chlorella as algae species) into CO of tube-pond combined flash bioreactor system 2 In the dissolver, the inoculation mass ratio of the microalgae culture solution to the water body in the whole tube-pond combined reactor is 7%. Introducing CO into the microalgae culture solution 2 And after the dissolver is started, a centrifugal pump is started, the algae liquid is pumped into the upper layer multi-group horizontal tube type reactor for photosynthesis growth, and then the algae liquid is sent into the lower layer runway pool for photosynthesis growth. CO from industrial flue gas 2 (from the exhaust flue gas of an industrial kiln, CO) 2 30% by volume of CO 2 The gas distributor in the dissolver is sent into the upper horizontal cross pipe and simultaneously the industrial flue gas CO 2 Feeding the waste gas into a runway pool through a membrane aerator to control CO of industrial flue gas 2 The aeration rate of the flow compared to the water in the whole tube-pool combined reactor was 0.04vvm. The intensity of the incident sunlight was 30000Lux and the ambient temperature was 25 ℃. The algae liquid circularly flows between the upper horizontal transverse pipe and the lower runway pool, and biomass is harvested after photosynthesis growth is carried out for 4 days.
Compared with the traditional simple runway pool reactor (a horizontal transverse pipe is not arranged above the runway pool reactor), the pipe pool combined type flashing bioreactor system is adopted to improve the flashing frequency of the movement of microalgae cells by 23.9%, the photochemical efficiency (Fv/Fm) of a light system II (PS II) by 8.6%, the chlorophyll a and chlorophyll b contents of the microalgae cells by 14.3% and 8.9%, the growth rate of the microalgae by 29.2% and the yield of the microalgae biomass of unit occupied area by 32.7%.
Example 3
The tube-pool combined flash bioreactor system is formed by combining an upper horizontal cross tube and a lower runway pool, and the horizontal cross tube and the runway pool are crossed. 1000 rows of horizontal transverse pipes are arranged above the length direction of the runway pool, and 10 runway pools are arranged below the length direction of the horizontal transverse pipes. The distance between the bottom of the horizontal transverse pipe and the bottom of the runway pool is 2 meters. The upper horizontal tube adopts a multi-layer horizontal tube structure formed by vertically stacking, the tube diameters of the upper horizontal tube are all 5 cm, and the upper horizontal tube is made of light-transmitting tubes (namely toughened glass with light transmittance of more than 80%). The total number of layers of the horizontal transverse pipes is 5, the pipe wall distance between two adjacent pipes in the vertical direction is 5 times of the pipe diameter, and the pipe wall distance between two adjacent pipes in the horizontal direction is 1 time of the pipe diameter. The upper horizontal cross tube reactor equipment comprises CO 2 Dissolver, multi-group horizontal transverse pipe and algae liquid circulation system, wherein CO made of stainless steel material 2 The dissolver is a gas-liquid mixing device, and the internal gas distributor is connected to the CO through a pipeline 2 The device is characterized in that an algae liquid is inoculated to an inlet of a horizontal transverse tube; the algae liquid circulation system comprises a centrifugal pump and a connecting pipeline, which connects a horizontal pipe with CO 2 The dissolver is connected in a sealing way and is used for driving the algae liquid to be connected with CO in a horizontal transverse pipe 2 Circulation in the dissolver. The lower layer runway pool reactor equipment comprises a runway pool, a stirring paddle wheel and a gas supply system, wherein the stirring paddle wheel is a cross paddle wheel made of stainless steel; the gas supply system is a rubber membrane aerator and a connecting pipeline thereof, the aperture of the aerator is 0.8mm, and the porosity is 0.85. When the incident sunlight vertically irradiates the reactor system, the light is absorbed and utilized by the upper horizontal transverse pipe and the lower runway pool in turn, and meanwhile, a region with alternate brightness is formed on the surface of the runway pool in the flowing direction of the algae liquid, so that a flash effect for promoting the growth of the microalgae cells when the microalgae cells in the runway pool flow is formed。
The method for realizing growth and carbon fixation of microalgae by utilizing a tube-pond combined flash bioreactor system comprises the following steps: inoculating microalgae culture solution (spirulina is the algae) into CO of tube-pond combined flash bioreactor system 2 In the dissolver, the inoculation mass ratio of the microalgae culture solution to the water body in the whole tube-pond combined reactor is 10%. Introducing CO into the microalgae culture solution 2 And after the dissolver is started, a centrifugal pump is started, the algae liquid is pumped into the upper layer multi-group horizontal tube type reactor for photosynthesis growth, and then the algae liquid is sent into the lower layer runway pool for photosynthesis growth. CO from industrial flue gas 2 (from flue gas discharged from coal chemical plant, CO) 2 99% by volume of CO 2 The gas distributor in the dissolver is sent into the upper horizontal cross pipe and simultaneously the industrial flue gas CO 2 Feeding the waste gas into a runway pool through a membrane aerator to control CO of industrial flue gas 2 The aeration rate of the flow compared to the water in the whole tube-pool combined reactor was 0.1vvm. The illumination intensity of the incident sunlight was 100000Lux and the ambient temperature was 38 ℃. The algae liquid circularly flows between the upper horizontal transverse pipe and the lower runway pool, and biomass is harvested after photosynthesis growth is carried out for 5 days.
Compared with the traditional simple runway pool reactor (a horizontal transverse pipe is not arranged above the runway pool reactor), the pipe pool combined type flashing bioreactor system is adopted to improve the flashing frequency of the movement of microalgae cells by 34.2%, the photochemical efficiency (Fv/Fm) of a light system II (PS II) is improved by 12.1%, the chlorophyll a and chlorophyll b contents of the microalgae cells are respectively improved by 21.1% and 11.2%, the growth rate of the microalgae is improved by 36.9%, and the yield of the microalgae biomass of unit occupied area is improved by 41.2%.
Finally, it should be noted that the above list is only specific embodiments of the present invention. Obviously, the invention is not limited to the above embodiments, but many variations are possible. All modifications directly derived or suggested to one skilled in the art from the present disclosure should be considered as being within the scope of the present invention.
Claims (9)
1. A tube-pool combined flash bioreactor system,comprises a runway pool reactor for culturing microalgae; the method is characterized in that a plurality of horizontal transverse pipes made of light-transmitting materials are arranged above the runway pool reactor in parallel, and the horizontal transverse pipes are mutually perpendicular to the length direction of the runway pool reactor; the end parts of the adjacent horizontal transverse pipes are connected by bent pipes to form a through horizontal transverse pipe reactor; the outlet end of the horizontal tube reactor is sequentially connected with the runway pool reactor and the CO through pipelines 2 Dissolver, CO 2 The outlet end of the dissolver is sequentially connected with the centrifugal pump and the inlet end of the horizontal cross tube reactor through a pipeline; in CO 2 The dissolver is provided with a gas distributor which is connected to the CO through a pipeline 2 A gas source;
the horizontal transverse pipes are overlapped in the vertical direction, at least 2 layers are arranged, the number of the horizontal transverse pipes in each layer is 10-1000 columns, and the number of the horizontal transverse pipes in each layer is not more than 5 layers; the horizontal pipes of the multiple layers are overlapped layer by layer in the vertical direction to form a row, so that the gaps of the horizontal pipes of each row in the horizontal direction are prevented from being blocked due to the staggered arrangement; the pipe wall distance between two adjacent pipes in the vertical direction is 1-5 times of the pipe diameter, and the pipe wall distance between two adjacent pipes in the horizontal direction is 1-10 times of the pipe diameter.
2. The system of claim 1, wherein the number of raceway pond reactors is 3-10; the runway pool reactors are arranged in a manner that the length directions are parallel to each other and are connected with each other end to end in sequence; each runway pool reactor is internally provided with a stirring paddle wheel and a membrane aerator.
3. The system of claim 2, wherein the agitator paddle wheel is a stainless steel cross paddle wheel; the membrane aerator is a rubber material product, the aperture is 0.8mm, and the porosity is 0.85; the membrane aerator is connected with CO through a pipeline 2 And (5) an air source.
4. The system of claim 1, wherein the horizontal cross pipe has a pipe diameter of 5 cm and a minimum distance between the bottom of the horizontal cross pipe and the bottom of the raceway pond is 2 meters.
5. The system of claim 1, wherein the horizontal cross tube is a plain glass tube, a tempered glass tube, or a polymeric plastic tube having a light transmittance of 80% or more.
6. A method for achieving growth and carbon sequestration of microalgae using the tube pond integrated flash bioreactor system of claim 1, comprising the steps of:
(1) Inoculating microalgae culture solution into CO 2 The dissolver calculates the inoculation mass ratio of the microalgae culture solution to be 5-10% by using the whole water body of the system; starting a centrifugal pump to enable algae liquid to enter the horizontal tube reactor at the upper layer for photosynthesis, and then enter the runway pool reactor at the lower layer for continuous growth and carbon fixation;
(2) CO from industrial flue gas 2 Simultaneously feeding CO 2 Gas distributor in dissolver and diaphragm aerator in runway pool reactor, forming NaHCO by carbonation reaction 3 To increase CO 2 The utilization efficiency; controlling CO 2 The flow rate is controlled to be 0.02-0.1 vvm compared with the ventilation rate of the whole water body of the system;
(3) The algae liquid circularly flows between the horizontal tube reactor and the runway pool reactor, and incident light is absorbed and utilized by the algae liquid in the two reactors; because the algae liquid can generate turbulence and waves when flowing in the horizontal tube reactor, after the incident light rays penetrate through the horizontal tube reactor on the upper layer, a region with alternate brightness is formed on the surface of the runway pool reactor, so that a flashing effect is formed to promote the growth of microalgae cells; after 3-5 days of system operation, biomass is harvested.
7. The method of claim 6, wherein the ambient temperature of the whole system is controlled to be 10-38 ℃, and the illumination intensity of the incident light on the upper surface of the horizontal tube reactor is 5000-100000Lux.
8. The method according to claim 6, wherein the microalgae species used in the microalgae culture solution is nannochloropsis, chlorella or spirulina.
9. The method of claim 6, wherein the industrial flue gas CO 2 Refers to the exhaust gas of coal-fired power plants, the tail gas of coal chemical plants or the exhaust gas of industrial furnaces, wherein CO 2 The volume concentration is 10-99%.
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