CN105779271A - Stepped drop type microalgae photoreactor - Google Patents

Stepped drop type microalgae photoreactor Download PDF

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Publication number
CN105779271A
CN105779271A CN201610333929.5A CN201610333929A CN105779271A CN 105779271 A CN105779271 A CN 105779271A CN 201610333929 A CN201610333929 A CN 201610333929A CN 105779271 A CN105779271 A CN 105779271A
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algae
declines
photoreactor
algae solution
ladder
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孙旭阳
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02Photobioreactors
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/22Transparent or translucent parts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/58Reaction vessels connected in series or in parallel
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/18External loop; Means for reintroduction of fermented biomass or liquid percolate
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M31/00Means for providing, directing, scattering or concentrating light
    • C12M31/08Means for providing, directing, scattering or concentrating light by conducting or reflecting elements located inside the reactor or in its structure
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/14Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes

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Abstract

The invention relates to the field of microalgae biotechnology and particularly relates to a stepped drop type microalgae photoreactor. The stepped drop type microalgae photoreactor comprises multiple step systems and a ditch system, wherein each step system comprises multiple steps arranged in a drop hierarchical manner; an overflow port is formed between every two adjacent upper and lower steps; a baffle for blocking is arranged at the two ends of each step respectively; the ditch system comprises an algae liquid collection device and an algae liquid conveying power device; the algae liquid collection device and the algae liquid conveying power device are communicated through a pipeline; a flow outlet is formed in the bottom step; the algae liquid collection device is arranged at the flow outlet; and the algae liquid in the algae liquid collection device is circulated into the top step through the algae liquid conveying power device.

Description

A kind of step therein declines algae Photoreactor
Technical field
The present invention relates to technical field of microalga biology, a kind of step therein declines algae Photoreactor specifically.
Background technology
Microalgae is the organism that photosynthetic efficiency is the highest, the multiple high added value material such as rich in proteins, polyunsaturated fatty acid and carotenoid, and microalgae has absorbed nitrogen/phosphorus simultaneously, and accumulates the ability of oils and fats in incubation.Therefore, microalgae has a wide range of applications in all many-sides such as food, bait, feedstuff, environmental protection and bioenergies, and particularly in recent years in bioenergy, microalgae is considered as raw material prepared by the bio-fuels such as the biodiesel of most application prospect;Additionally, microalgae carbon sequestration and microalgae process also very huge rich in the potentiality of nitrogen/phosphorus waste water.
Bioreactor is the core apparatus that microalgae light autotrophy is cultivated.The bioreactor being presently used for the cultivation of microalgae light autotrophy has open type and closed two kinds.
The advantages such as open type microalgae culture system (raceway pond and circle pond) also exists simple in construction, it is easy, easy to operate to build and operating cost is low.But, open type pond also exists more shortcoming, as slow in frustule growth, algae cell density relatively low (usual algae cell density < 1.0g/L), being subject to the impact of external environmental condition, condition of culture is difficult to control to, it is easy to be subject to the pollution of the harmful organisms such as protozoacide.Meanwhile, relatively low algae cell density can cause higher cost of gathering.Compared with open type microalgae culture system, closed photo bioreactor has condition of culture and stablizes controlled, frustule growth rate is fast and algae cell density advantages of higher, but closed photo bioreactor also exists investment, runs and the problem such as maintenance cost height and amplification difficulty so that they are difficult to large-scale application at present and cultivate in the light autotrophy of non-high added value microalgae (energy microalgae).Therefore, closed photo bioreactor is currently mainly applied to the algal species cultivation of microalgae and the cultivation of high added value microalgae (such as Haematocoocus Pluvialls).
The light path of bioreactor and unit volume illuminating area are the key factors affecting reactor performance.It is said that in general, light path is more short and the illuminating area of unit volume algae solution is more big, the performance of bioreactor is more excellent, and the growth rate of frustule is more fast, and algae cell density is more high.At present, in open type pond, the algae solution degree of depth is generally about 15 ~ 30cm, and can fully accept the region of illumination only on the top layer of algae solution, only about several centimetres, most frustule is in dark space (the minimum light intensity that light intensity is required lower than frustule growth), illumination cannot be accepted fully, cause that frustule cannot be carried out photosynthesis and fast-growth effectively.Closed photo bioreactor, such as tubular type bioreactor, the light path of pillar bioreactor and flat plate photobioreactor is typically in about 5cm ~ 20cm.Compared with open type culture systems, the light path of closed photo bioreactor is relatively small, bigger than illuminating area.Although, frustule can obtain higher density in closed photo bioreactor, but the equipment investment of enclosed bioreactor is very big, and power consumption rate is high, it is difficult to scale is applied to the cultivation of general low value microalgae.Meanwhile, under higher algae cell density, light attenuation degree in algae solution increases, and is generally only capable of penetrating algae solution layer about 1-2cm.Therefore, the light path of closed photo bioreactor is still excessive, and under higher algae cell density, major part frustule is subject to light restriction still in dark space.
Light receiving surface in order to increase microalgae culture system amasss, and improves the luminous energy that single algae cell obtains, and some researcheres are taken measures on customs clearance and microalgae culture system has been transformed.
In order to make full use of spatial light photograph to a greater degree, improve the frustule realize micro-algae large-scale, high density and high efficiency to the utilization ratio of luminous energy, low cost and cultivate.Therefore, this area need nonetheless remain for a kind of simple, low cost and has the bigger Novel microalgae culture system than illuminating area.
Summary of the invention
The present invention is for providing a kind of simple in construction, reducing algae solution thickness in microalgae light autotrophy incubation, increase algae solution light-receiving area, make frustule energy fully receiving space illumination, algae solution transmitting power efficiency is high, it is possible to achieve the step therein that the low cost of microalgae, high density and high efficiency are cultivated declines algae Photoreactor.
The present invention is achieved through the following technical solutions:
A kind of step therein declines algae Photoreactor, including several ladder system and sewerage, each ladder system includes several ladders being drop height type layering and arranging, it is respectively equipped with overfall between adjacent upper and lower two ladders of each two, the two ends of each ladder are respectively provided with baffle plate and stop, described sewerage includes algae solution collection device and algae solution power delivery unit, described algae solution collection device is connected by pipeline with algae solution power delivery unit, the ladder of bottom is provided with flow export, described algae solution collection device is located at described flow export place, by algae solution power delivery unit, the algae solution in algae solution collection device is circulated to the ladder of the top.
The ladder of the top is provided with inflow entrance, and between the inflow entrance of top, several middle overfalls and lower section flow export are adjacent between two, left and right is crisscross arranged mutually.
It is provided with clear layer on the microalgae liquid level of described ladder, between clear layer and ladder, forms cavity;Or the step therein algae Photoreactor that declines is provided externally with clear layer.
The inner surface of described ladder is provided with reflector layer, reflective for microalgae absorption to increase.
It is provided with filter element, to realize effectively concentrating or gathering microalgae in described algae solution collection device.
Described algae solution power delivery unit include algae solution collecting tank, pump, for the pipeline connected and intelligent regulating system, described algae solution collecting tank is located at described flow export place, described pump is connected by pipeline with algae solution collecting tank, and described intelligent regulating system electrically connects with described pump.
The described step therein algae Photoreactor that declines also includes aerating system, and described aerating system includes air pump and breather, and described breather stretches in ladder system, and described air pump electrically connects with described intelligent regulating system;Described algae solution power delivery unit also includes storage tank, and the algae solution that described algae solution collecting tank is collected flows in storage tank, is conveyed upwards in the ladder of the top by pump by the algae solution in storage tank, and described breather stretches in described storage tank.
On described pipeline and breather is equipped with valve.
The liquid level of microalgae is 1-30cm.
The liquid level of microalgae is 3-10cm.
Described overfall place is provided with plate, and described plate moves up and down or move left and right the size adjustment realizing overfall, and then regulates algae solution height and uninterrupted, and described plate adopts and manually or automatically regulates.
Described step therein declines the drop angle of algae Photoreactor between 10 °-75 °.
Described step therein declines the drop angle of algae Photoreactor between 25 °-60 °.
The present invention is had the benefit that
In the present invention, the described step therein algae Photoreactor that declines includes several ladder system and sewerage, each ladder system includes several ladders being drop height type layering and arranging, algae solution flow to following ladder from ladder above successively, again through sewerage by algae solution circulation to the ladder of the superiors, circulate successively.Described ladder can be horizontally disposed with or be obliquely installed, can arrange according to the illumination rule of local latitudes different on the earth to realize maximum light radiation, the height particularly controlling algae solution face is consistent with effective light path, and manually or automatically can regulate according to intensity of illumination in the daytime so that the luminous energy that the frustule of cultivation receives reaches optimum level;Mechanical lifting and pumping make the effective power efficiency that algae solution is transported be much higher than open type and original closed culture systems plus automatic overflow, most of algae solution that original culture systems power transports is in dark space, effective algae solution two generally only has 10%-20%, open type is often below 5%, and closed culture systems algae solution is bigger with the contact area of wall, cause that frictional resistance increases, kinetic equation loss is serious, and the conveying effective percentage of the present invention is up to 50%-90%, and magnanimity lifting resistance is little, efficiency is high.The step therein algae reactor that declines that the present invention adopts can adopt cheap structural member and wallboard, such as plastics, iron and steel, cement, timber, glass etc., construction cost is low, pumping efficiency is high and then brings operating cost low, the natural land system such as hillside fields, terraced fields and condition can also be used, greatly save construction cost.
Accompanying drawing explanation
Below in conjunction with accompanying drawing, the present invention is described in further detail.
Fig. 1 is that step therein of the present invention declines the structural representation of algae Photoreactor first embodiment.
Fig. 2 is that step therein of the present invention declines the structural representation of algae Photoreactor the second embodiment.
Fig. 3 is that step therein of the present invention declines the structural representation of algae Photoreactor the 3rd embodiment.
The label that in figure, component names is corresponding is as follows:
1, ladder;2, overfall;3, baffle plate;4, pipeline;5, flow export;6, inflow entrance;7, filter element;8, algae solution collecting tank;9, pump;10, intelligent regulating system;11, air pump;12, breather;13, storage tank;14, valve;15, plate;16, clear layer.
Detailed description of the invention
Below in conjunction with drawings and Examples, the present invention is described in further detail:
Embodiment one:
Decline the embodiment of algae Photoreactor as step therein of the present invention, as schematically shown in Figure 1, including three ladder system and sewerage, each ladder system includes four ladders 1 that layering is arranged in drop height type, it is respectively equipped with overfall 2 between adjacent upper and lower two ladders of each two, the two ends of each ladder 1 are respectively provided with baffle plate 3 and stop, described sewerage includes algae solution collection device and algae solution power delivery unit, described algae solution collection device is connected by pipeline 4 with algae solution power delivery unit, the ladder of bottom is provided with flow export 5, described algae solution collection device is located at described flow export 5 place, by algae solution power delivery unit, the algae solution in algae solution collection device is circulated to the ladder 1 of the top.
In the present embodiment, the ladder 1 of the top is provided with inflow entrance 6, and between the inflow entrance 6 of top, three middle overfalls 2 and lower section flow export 5 are adjacent between two, left and right is crisscross arranged mutually.So it is crisscross arranged, it is possible to effectively make algae solution flow in ladder 1, and flows to smoothly in next ladder 1 or algae solution collection device.
In the present embodiment, the inner surface of described ladder 1 is provided with reflector layer (not shown), reflective for microalgae absorption to increase.
In the present embodiment, in described algae solution collection device, it is provided with filter element 7, to realize effectively concentrating or gathering microalgae.
In the present embodiment, described algae solution power delivery unit include algae solution collecting tank 8, pump 9, for the pipeline 4 connected and intelligent regulating system 10, described algae solution collecting tank 8 is located at described flow export 5 place, described pump 9 is connected by pipeline 4 with algae solution collecting tank 8, and described intelligent regulating system 10 electrically connects with described pump 9.
In the present embodiment, the described step therein algae Photoreactor that declines also includes aerating system, and described aerating system includes air pump 11 and breather 12, and described breather 12 stretches in ladder system, and described air pump 11 electrically connects with described intelligent regulating system 10;Described algae solution power delivery unit also includes storage tank 13, and the algae solution that described algae solution collecting tank 8 is collected flows in storage tank 13, is conveyed upwards in the ladder 1 of the top by pump 9 by the algae solution in storage tank 13, and described breather 12 stretches in described storage tank 13.Timing supplements fresh culture medium or water in storage tank 13, to make up the water yield of evaporation on daytime.When frustule reaches certain density, it is in when harvest stages, stops supplementing fresh water in microalgae culture system, utilize the irradiation of sunlight, rely on the evaporation of moisture that frustule is concentrated, to realize gathering of frustule.
In the present embodiment, on described pipeline 4 and breather 12 is equipped with valve 14.Manually control valve 14 is to regulate algae solution flow, maybe can also automatically control pump 9 and valve 14 to regulate algae solution flow by intelligent regulating system 10 according to intensity of illumination, temperature, frustule concentration.So can realizing according to many factors complex optimums such as illumination condition, algae solution layer thickness, temperature and water temperature, frustule concentration, frustule growth stages, reaching maximum frustule Biomass increases.
In the present embodiment, the liquid level of microalgae is 5cm.
In the present embodiment, described overfall 2 place is provided with plate 15, and described plate 15 moves left and right the size adjustment realizing overfall, and then regulates algae solution height and uninterrupted, and described plate 15 adopts manual adjustment.
In the present embodiment, the decline drop angle of algae Photoreactor of described step therein is 30 °.
Certainly, the described step therein algae Photoreactor that declines can also include the ladder system of one or two or several rows or some row or array arrangement, then passes through sewerage and each ladder system is connected;Each ladder system can also include the ladder that layering is arranged in drop height type of any number between three to 50;Described plate can also move up and down the size adjustment realizing overfall;Described plate can also adopt the mode being automatically adjusted.So, also all within scope, no longer repeat one by one here.
Embodiment two:
Decline the embodiment of algae Photoreactor as step therein of the present invention, as in figure 2 it is shown, in the present embodiment, the step therein algae Photoreactor that declines is provided externally with clear layer 16.The whole clear layer 16 algae Photoreactor that whole step therein declined wraps up.
In the present embodiment, the liquid level of microalgae is 30cm.
In the present embodiment, the decline drop angle of algae Photoreactor of described step therein is 10 °.
Certainly, the inner bottom surface of described ladder can also arrange the longitudinal or horizontal and vertical current-limiting components being crisscross arranged.
In the present embodiment, all the other structures and beneficial effect are all consistent with embodiment one, no longer repeat one by one here.
Embodiment three:
Decline the embodiment of algae Photoreactor as step therein of the present invention, as it is shown on figure 3, in the present embodiment, be provided with clear layer 16 on the microalgae liquid level of described ladder 1, forms cavity between clear layer 16 and ladder 1.Clear layer 16 is possible not only to form closed system so that the cultivation of microalgae, reduce external environment, such as dust storm, rainwater, the adverse effect that frustule is cultivated by assorted algae etc., reduces evaporation of water amount, and can form the bottom of cavity at ladder 1 and clear layer 16 and pass into the mixing gas of pure CO2 or CO2 and air, the gas floated and the algae solution of flow down form reverse flow in cavity body, significantly increase gas-liquid mass transfer and strengthen the mixing of algae solution.
In the present embodiment, the liquid level of microalgae is 1cm.
In the present embodiment, the decline drop angle of algae Photoreactor of described step therein is 75 °.
In the present embodiment, all the other structures and beneficial effect are all consistent with embodiment one, no longer repeat one by one here.
In the present invention, described algae solution power delivery unit can consider the modes such as positive displacement promotes, such as waterwheel, can reduce the pump destruction to frustule wall.So, also all within scope, no longer repeat one by one here.

Claims (13)

1. a step therein declines algae Photoreactor, it is characterized in that including several ladder system and sewerage, each ladder system includes several ladders being drop height type layering and arranging, it is respectively equipped with overfall between adjacent upper and lower two ladders of each two, the two ends of each ladder are respectively provided with baffle plate and stop, described sewerage includes algae solution collection device and algae solution power delivery unit, described algae solution collection device is connected by pipeline with algae solution power delivery unit, the ladder of bottom is provided with flow export, described algae solution collection device is located at described flow export place, by algae solution power delivery unit, the algae solution in algae solution collection device is circulated to the ladder of the top.
2. step therein as claimed in claim 1 declines algae Photoreactor, it is characterised in that the ladder of the top is provided with inflow entrance, and between the inflow entrance of top, several middle overfalls and lower section flow export are adjacent between two, left and right is crisscross arranged mutually.
3. step therein as claimed in claim 1 declines algae Photoreactor, it is characterised in that is provided with clear layer on the microalgae liquid level of described ladder, forms cavity between clear layer and ladder;Or the step therein algae Photoreactor that declines is provided externally with clear layer.
4. step therein as claimed in claim 1 declines algae Photoreactor, it is characterised in that the inner surface of described ladder is provided with reflector layer, reflective absorbs for microalgae to increase.
5. step therein as claimed in claim 1 declines algae Photoreactor, it is characterised in that be provided with filter element in described algae solution collection device, to realize effectively concentrating or gathering microalgae.
6. step therein as claimed in claim 1 declines algae Photoreactor, it is characterized in that described algae solution collection device includes algae solution collecting tank, described algae solution power delivery unit includes pump, for the pipeline connected and intelligent regulating system, described algae solution collecting tank is located at described flow export place, described pump is connected by pipeline with algae solution collecting tank, and described intelligent regulating system electrically connects with described pump.
7. step therein as claimed in claim 6 declines algae Photoreactor, it is characterized in that the described step therein algae Photoreactor that declines also includes aerating system, described aerating system includes air pump and breather, and described breather stretches in ladder system, and described air pump electrically connects with described intelligent regulating system;Described algae solution power delivery unit also includes storage tank, and the algae solution that described algae solution collecting tank is collected flows in storage tank, is conveyed upwards in the ladder of the top by pump by the algae solution in storage tank, and described breather stretches in described storage tank.
8. step therein as claimed in claim 8 declines algae Photoreactor, it is characterised in that on described pipeline and be equipped with valve on breather.
9. step therein as claimed in claim 1 declines algae Photoreactor, it is characterised in that the liquid level of microalgae is 1-30cm.
10. step therein as claimed in claim 9 declines algae Photoreactor, it is characterised in that the liquid level of microalgae is 3-10cm.
The algae Photoreactor 11. step therein as claimed in claim 1 declines, it is characterized in that described overfall place is provided with plate, described plate moves up and down or moves left and right the size adjustment realizing overfall, and then regulates algae solution height and uninterrupted, and described plate adopts and manually or automatically regulates.
The algae Photoreactor 12. step therein as claimed in claim 1 declines, it is characterised in that described step therein declines the drop angle of algae Photoreactor between 10 °-75 °.
The algae Photoreactor 13. step therein as claimed in claim 12 declines, it is characterised in that described step therein declines the drop angle of algae Photoreactor between 25 °-60 °.
CN201610333929.5A 2016-05-19 2016-05-19 Stepped drop type microalgae photoreactor Pending CN105779271A (en)

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CN111500462A (en) * 2020-04-13 2020-08-07 青岛旭能生物工程有限责任公司 Method for industrial culture of nannochloropsis
CN112375652A (en) * 2020-11-17 2021-02-19 广西小藻农业科技有限公司 Light biological reaction device and system
GB2599342A (en) * 2020-07-31 2022-04-06 Narasimhamurthy Prakashkumar Bioreactor for cell culture

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Publication number Priority date Publication date Assignee Title
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GB2599342A (en) * 2020-07-31 2022-04-06 Narasimhamurthy Prakashkumar Bioreactor for cell culture
CN112375652A (en) * 2020-11-17 2021-02-19 广西小藻农业科技有限公司 Light biological reaction device and system

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Application publication date: 20160720