CN212504872U - Tube-pool combined flash bioreactor system - Google Patents
Tube-pool combined flash bioreactor system Download PDFInfo
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- CN212504872U CN212504872U CN202021359971.2U CN202021359971U CN212504872U CN 212504872 U CN212504872 U CN 212504872U CN 202021359971 U CN202021359971 U CN 202021359971U CN 212504872 U CN212504872 U CN 212504872U
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Abstract
The utility model relates to a biomass energy utilization technology, aiming at providing a tube-pool combined flash bioreactor system. The system includes a raceway pond reactor for culturing microalgae; a plurality of horizontal transverse pipes made of light-transmitting materials are arranged above the runway pool reactor in parallel, and the length directions of the horizontal transverse pipes and the runway pool reactor are mutually vertical; the end parts of the adjacent horizontal transverse pipes are connected by a bent pipe to form a through horizontal transverse pipe reactor; the outlet end of the horizontal transverse tube reactor is sequentially connected with the raceway pond reactor and CO through a pipeline2The outlet end of the dissolver is sequentially connected with the centrifugal pump and the inlet end of the horizontal transverse tube reactor through pipelines; in CO2The dissolver is provided with a gas distributor which is connected to the CO through a pipeline2And (4) a gas source. The utility model discloses can improve the light zone and the dark space distribution on runway pond algae liquid surface, show the flash of light effect that strengthens little algae cell flow, improve the inside flash of light frequency of little algae cell motion in runway pond, be favorable to promoting little algae photosynthesis growth solid carbon.
Description
Technical Field
The utility model relates to a biomass energy utilization technology, in particular to a tube-pool combined flash bioreactor system.
Background
Microalgae has become flue gas CO due to high growth speed, high light energy utilization efficiency and high economic value of biomass2Leading edge of the fields of emission reduction and new energy developmentThe research focus. Microalgae is fully utilized to capture CO in flue gas discharged by coal-fired power plants, coal chemical plants, industrial furnaces and the like2The cost of raw materials for producing the microalgae is reduced, and meanwhile, the biomass rich in high-value active ingredients can be obtained. The runway pool reactor is used as the most successful commercial culture mode of the microalgae culture industry, and the equipment and process are simple to operate and easy to amplify, so that the runway pool reactor is widely applied at home and abroad.
The penetration distance of the sunlight in the algae liquid is very limited, and the microalgae cells at the bottom of the raceway pond are far away from the light receiving surface of the algae liquid, so that sufficient light energy is difficult to obtain for photosynthesis. However, when the sunlight of the external environment is too strong, the microalgae cells on the surface of the raceway pond algae liquid are exposed to strong light for a long time, so that the light energy is forced to be received more than the utilization amount of the cell photosynthesis system, and the cell photosynthesis efficiency is reduced. The researchers defined the reactor zones with light intensity below a certain threshold as dark zones, and vice versa as light zones. Research finds that microalgae needs to be rapidly changed between a light area and a dark area in a culture solution of a reactor continuously to obtain higher light energy utilization efficiency and cell growth speed. Therefore, the number of times of the transformation movement of the microalgae cells between the light area and the dark area in each second is called as the flash frequency, and the flash frequency of the movement of the microalgae cells can be effectively improved by improving the internal structure of the reactor and optimizing the distribution characteristics of the light area and the dark area. Yang et al set up the vortex flow field of vortex flow baffle production in the runway pond inside to strengthen little algae motion's flash frequency and improve growth rate, promote gas-liquid mixture mass transfer through reactor structure optimization and make little algae living beings output improve 22%. However, the flow disturbance baffle increases the flow resistance and reduces the flow speed of the microalgae liquid, so a novel reactor structure is needed to be developed to improve the flash frequency and promote the growth and carbon fixation of the microalgae.
SUMMERY OF THE UTILITY MODEL
The to-be-solved technical problem of the utility model is to overcome the not enough among the prior art, provide a tube-pool convolution flash of light bioreactor system.
In order to solve the technical problem, the utility model discloses a solution is:
provides a tube pool combined flashA bioreactor system comprising a raceway pond reactor for culturing microalgae; the device is characterized in that a plurality of horizontal transverse pipes made of light-transmitting materials are arranged above the raceway pond reactor in parallel, and the horizontal transverse pipes are vertical to the length direction of the raceway pond reactor; the end parts of the adjacent horizontal transverse pipes are connected by a bent pipe to form a through horizontal transverse pipe reactor; the outlet end of the horizontal transverse tube reactor is sequentially connected with the raceway pond reactor and CO through a pipeline2Dissolver, CO2The outlet end of the dissolver is sequentially connected with the centrifugal pump and the inlet end of the horizontal transverse tube reactor through pipelines; in CO2The dissolver is provided with a gas distributor which is connected to the CO through a pipeline2And (4) a gas source.
As an improvement, the horizontal transverse pipes are divided into 1-5 layers and are arranged in an overlapped mode along the vertical direction, and the number of the transverse pipes in each layer is 10-1000 rows; the pipe wall space between two adjacent pipes in the vertical direction is 1-5 times of the pipe diameter, and the pipe wall space between two adjacent pipes in the horizontal direction is 1-10 times of the pipe diameter.
As an improvement, the number of the runway pool reactors is 3-10; the runway pool reactors are arranged in a mode that the length directions are mutually parallel and are sequentially connected end to end; each raceway pond reactor is internally provided with a stirring paddle wheel and a membrane aerator.
As an improvement, the stirring paddle wheel is a cross paddle wheel made of stainless steel; the membrane aerator is made of rubber, the aperture is 0.8mm, and the porosity is 0.85; the membrane aerator is connected with CO through a pipeline2And (4) a gas source.
As an improvement, the diameter of the horizontal transverse pipe is 5 cm, and the minimum distance between the bottom of the horizontal transverse pipe and the bottom of the runway pool is 2 m.
As an improvement, the horizontal transverse pipe is a common glass pipe, a toughened glass pipe or a polymer plastic pipe with the light transmittance of more than 80%.
The method for realizing microalgae growth carbon sequestration by using the pipe-pool combined flash bioreactor system comprises the following steps:
(1) inoculating microalgae culture solution into CO2A dissolver for calculating microalgae culture with the whole water body of the systemThe inoculation mass ratio of the liquid is 5-10%; starting a centrifugal pump to enable the algae liquid to enter the upper horizontal cross-tube reactor for photosynthesis, and then enter the lower raceway pond reactor for continuous carbon fixation;
(2) CO in industrial flue gas2CO is simultaneously fed in2Gas distributor in dissolver, and membrane aerator in raceway pond reactor for forming NaHCO by carbonation reaction3To increase CO2Utilization efficiency; control of CO2The flow rate is controlled to be 0.02-0.1 vvm compared with the total water body of the system;
(3) the algae liquid circularly flows between the horizontal cross-tube reactor and the raceway pond reactor, and incident light is absorbed and utilized by the algae liquid in the two reactors; because the algae liquid can generate turbulence and waves when flowing in the horizontal cross-tube reactor, after incident light penetrates through the upper horizontal cross-tube reactor, light and shade alternate areas are formed on the surface of the runway pool reactor, so that a flash effect is formed to promote the growth of microalgae cells; and harvesting the biomass after the system runs for 3-5 days.
The environmental temperature of the whole system is controlled to be 10-38 ℃, and the illumination intensity of the incident light on the upper surface of the horizontal cross-tube reactor is 5000-. The microalgae used in the microalgae culture solution can be nannochloropsis, chlorella or spirulina. Industrial flue gas CO2Can be selected from flue gas discharged by coal-fired power plants, tail gas discharged by coal chemical plants or flue gas discharged by industrial furnaces and kilns, wherein CO is contained in the flue gas2The volume concentration is 10-99%.
Compared with the prior art, the beneficial effects of the utility model are that:
1. the tube-pool combined flash bioreactor system obviously improves the distribution of light areas and dark areas on the surface of the microalgae liquid in the raceway pool, obviously enhances the flash effect of microalgae cell flow, improves the flash frequency of movement of microalgae cells in the raceway pool by 12.7-34.2 percent, and is favorable for promoting the photosynthesis, growth and carbon fixation of microalgae.
2. Because the tube-pool combined photoreactor comprises the special structure of the upper horizontal tube and the lower raceway pool, the distribution of the projection area of incident light quanta in the reactor is improved, the microalgae circulating in the reactor is prevented from being exposed to excessive light for a long time, and the photoinhibition effect of strong light on microalgae cells is effectively relieved. Therefore, the photochemical efficiency (Fv/Fm) of a photosystem II (PS II) of the microalgae in the reactor is improved by 5.3-12.1%, the chlorophyll a content is improved by 11.6-21.1%, and the chlorophyll b content is improved by 6.3-11.2%, so that the microalgae can convert light energy into ATP more efficiently for synthesis of algae cytochrome and accumulation of biomass. Because the reactor system adopts a two-stage absorption mode for incident solar energy, and the incident solar energy is absorbed and utilized by the upper horizontal transverse pipe and the lower runway pool in sequence, the whole reactor system has high utilization rate of land resources and light energy, the growth rate of microalgae is improved by 21.5-36.9%, and the yield of microalgae biomass in unit floor area is improved by 24.8-41.2%.
Drawings
FIG. 1 is a perspective view of the tube-pool combined flash bioreactor system of the present invention.
Fig. 2 is a perspective view of the system from another perspective.
The reference numbers in the figures are: 1, a stirring paddle wheel; 2 a raceway pond reactor; 3, a membrane aerator; 4CO2A dissolver; 5, a gas distributor; 6 centrifugal pump; 7 a return pipe; 8, connecting pipes; 9 horizontal cross-tube reactor.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings and specific embodiments. The examples are intended to provide those skilled in the art with a more complete understanding of the present invention, and are not intended to limit the invention in any way.
As shown in fig. 1, above the length direction of the runway pool reactor 2, the horizontal transverse pipes are divided into 1-5 layers and are arranged in an overlapping manner along the vertical direction (i.e. the multiple layers of transverse pipes are arranged in an overlapping manner layer by layer in the vertical direction to form a row, so that the gaps of the transverse pipes in the horizontal direction are prevented from being shielded due to the disordered arrangement), and the number of the transverse pipes in each layer is 10-1000; the pipe wall space between two adjacent pipes in the vertical direction is 1-5 times of the pipe diameter, and the pipe wall space between two adjacent pipes in the horizontal direction is 1-10 times of the pipe diameter. 3-10 runway pool reactors 2 are arranged below the horizontal transverse pipe in the length direction, and the runway pool reactors 2 are arranged in a manner of being parallel to each other in the length direction and are sequentially connected end to end; each raceway pond reactor 2 is provided with a stirring paddle wheel 1 and a membrane aerator 3. The ends of the adjacent horizontal pipes are connected by a bent pipe to form a through horizontal pipe reactor 9.
Therefore, the tube-pool combined flash bioreactor system is formed by combining the upper horizontal cross-tube reactor 9 and the lower raceway-pool reactor 2, and the two reactors are in a cross shape. The outlet end of the horizontal cross-tube reactor 9 is sequentially connected with the runway pool reactor 2 and CO through pipelines2Dissolver 4, CO2The outlet end of the dissolver 4 is sequentially connected with the centrifugal pump 6 and the inlet end of the horizontal transverse tube reactor 9 through pipelines; in CO2 A gas distributor 5 is arranged in the dissolver 4, the gas distributor 5 is connected to the CO through a pipeline2And (4) a gas source.
The stirring paddle wheel 1 can be a cross paddle wheel made of stainless steel; the membrane aerator 3 is made of rubber, the aperture is 0.8mm, and the porosity is 0.85; the membrane aerator 3 is connected with CO through a pipeline2And (4) a gas source. The horizontal transverse tube can be a common glass tube, a toughened glass tube or a polymer plastic tube with the light transmittance of more than 80 percent. The pipe diameter of the horizontal transverse pipe can be selected to be 5 cm, and the minimum distance between the bottom of the horizontal transverse pipe and the bottom of the runway pool can be selected to be 2 m.
The method for realizing microalgae growth carbon sequestration by using the pipe-pool combined flash bioreactor system comprises the following steps:
(1) inoculating microalgae culture solution into CO2A dissolver, wherein the inoculation mass ratio of the microalgae culture solution is calculated by using the whole water body of the system to be 5-10%; starting a centrifugal pump to enable the algae liquid to enter the upper horizontal cross-tube reactor for photosynthesis, and then enter the lower raceway pond reactor for continuous carbon fixation;
(2) CO in industrial flue gas2CO is simultaneously fed in2Gas distributor in dissolver, and membrane aerator in raceway pond reactor for forming NaHCO by carbonation reaction3To increase CO2Utilization efficiency; control of CO2The flow rate is controlled to be 0.02-0.1 vvm compared with the total water body of the system;
(3) the algae liquid circularly flows between the horizontal cross-tube reactor and the raceway pond reactor, and incident light is absorbed and utilized by the algae liquid in the two reactors; because the algae liquid can generate turbulence and waves when flowing in the horizontal cross-tube reactor, after incident light penetrates through the upper horizontal cross-tube reactor, light and shade alternate areas are formed on the surface of the runway pool reactor, so that a flash effect is formed to promote the growth of microalgae cells; and harvesting the biomass after the system runs for 3-5 days.
The microalgae used in the microalgae culture solution can be selected from Nannochloropsis, Chlorella or Spirulina. Industrial flue gas CO2Can be selected from flue gas discharged by coal-fired power plants, tail gas discharged by coal chemical plants or flue gas discharged by industrial furnaces and kilns, wherein CO is contained in the flue gas2The volume concentration is 10-99%. The environmental temperature of the whole system is controlled to be 10-38 ℃, and the illumination intensity of the incident light on the upper surface of the horizontal cross-tube reactor is 5000-.
Example 1
The tube-pool combined flash bioreactor system is formed by combining an upper horizontal transverse tube with a lower runway pool, and the horizontal transverse tube and the runway pool are in a cross shape. 10 rows of horizontal transverse pipes are arranged above the length direction of the runway pool, and 3 runway pools are arranged below the horizontal transverse pipes in the length direction. The distance between the bottom of the horizontal transverse pipe and the bottom of the runway pool is 2 meters. The upper horizontal transverse pipe is vertically stacked to form a multi-layer transverse pipe structure, the pipe diameter of the upper horizontal transverse pipe is 5 cm, and the upper horizontal transverse pipe is made of light-transmitting pipes (namely high polymer pipes with light transmittance of more than 80%). The total number of layers of the horizontal transverse pipes is 1, and the pipe wall space between two adjacent pipes in the horizontal direction is 10 times of the pipe diameter. The upper horizontal cross-tube reactor equipment comprises CO2Dissolver, multiple groups of horizontal transverse pipes and algae liquid circulating system, wherein the dissolver is made of CO made of stainless steel material2The dissolver is a gas-liquid mixing device, and the gas distributor inside the dissolver is connected to CO through a pipeline2The air source is used for inoculating the algae liquid to the inlet of the horizontal tube; the algae liquid circulating system comprises a centrifugal pump and a connecting pipeline, and the horizontal transverse pipe and the CO are connected by the connecting pipeline2The dissolver is connected with the dissolver in a sealing way and is used for driving the algae liquid to be connected with CO in the horizontal transverse pipe2Circulating flow in the dissolver. The lower raceway pond reactor apparatus includesThe device comprises a runway pool, a stirring paddle wheel and a gas supply system, wherein the stirring paddle wheel is a cross paddle wheel made of stainless steel; the gas supply system is a rubber membrane aerator and a connecting pipeline thereof, the aperture of the aerator is 0.8mm, and the porosity is 0.85. When the incident sunlight vertically irradiates the reactor system, the light is sequentially absorbed and utilized by the upper horizontal transverse pipe and the lower raceway pond, and light and dark areas are formed in the flowing direction of the algae liquid on the surface of the raceway pond, so that a flash effect is formed when the microalgae cells in the raceway pond flow, and the growth of the microalgae cells is promoted.
The method for realizing microalgae growth carbon sequestration by using the tube-pool combined flash bioreactor system comprises the following steps: inoculating microalgae culture solution (microalgae is nannochloropsis oculata) to CO of tube-pool combined flash bioreactor system2In the dissolver, the inoculation mass ratio of the microalgae culture solution to the water body in the whole tube-pool combined reactor is 5%. Introducing CO into microalgae culture solution2And starting the centrifugal pump after the dissolver, pumping the algae solution into the upper-layer multi-group horizontal transverse tubular reactors for photosynthesis growth, and then sending the algae solution into the lower-layer raceway pond for photosynthesis growth. CO in industrial flue gas2(derived from coal-fired power plant exhaust fumes, CO2Volume concentration of 10%) by CO2A gas distributor inside the dissolver is sent into an upper horizontal transverse pipe and simultaneously sends industrial flue gas CO2Feeding the industrial flue gas into a runway pool through a membrane aerator to control the CO of the industrial flue gas2The aeration rate of the flow rate compared to the water in the entire tube-pool combined reactor was 0.02 vvm. The illumination intensity of incident sunlight is 5000Lux, and the ambient temperature is 10 ℃. The algae liquid circularly flows between the upper horizontal transverse pipe and the lower runway pool, and the biomass is harvested after the algae liquid grows for 3 days through photosynthesis.
Compared with the traditional simple raceway pond reactor (no horizontal pipe is arranged above the reactor), the tube-pond combined flash bioreactor system is adopted to improve the flash frequency of microalgae cell movement by 12.7%, the photochemical efficiency (Fv/Fm) of a photosystem II (PS II) by 5.3%, the contents of chlorophyll a and chlorophyll b in algae cells by 11.6% and 6.3%, respectively, the growth rate of microalgae by 21.5%, and the yield of microalgae biomass in unit floor area by 24.8%.
Example 2
The tube-pool combined flash bioreactor system is formed by combining an upper horizontal transverse tube with a lower runway pool, and the horizontal transverse tube and the runway pool are in a cross shape. 300 rows of horizontal transverse pipes are arranged above the length direction of the runway pool, and 6 runway pools are arranged below the horizontal transverse pipes in the length direction. The distance between the bottom of the horizontal transverse pipe and the bottom of the runway pool is 2 meters. The upper horizontal transverse pipe is vertically stacked to form a multi-layer transverse pipe structure, the pipe diameter of the upper horizontal transverse pipe is 5 cm, and the upper horizontal transverse pipe is made of light-transmitting pipes (namely common glass with light transmittance of more than 80%). The total number of layers of the horizontal transverse pipes is 2, the pipe wall distance between two adjacent pipes in the vertical direction is 1 time of the pipe diameter, and the pipe wall distance between two adjacent pipes in the horizontal direction is 6 times of the pipe diameter. The upper horizontal cross-tube reactor equipment comprises CO2Dissolver, multiple groups of horizontal transverse pipes and algae liquid circulating system, wherein the dissolver is made of CO made of stainless steel material2The dissolver is a gas-liquid mixing device, and the gas distributor inside the dissolver is connected to CO through a pipeline2The air source is used for inoculating the algae liquid to the inlet of the horizontal tube; the algae liquid circulating system comprises a centrifugal pump and a connecting pipeline, and the horizontal transverse pipe and the CO are connected by the connecting pipeline2The dissolver is connected with the dissolver in a sealing way and is used for driving the algae liquid to be connected with CO in the horizontal transverse pipe2Circulating flow in the dissolver. The lower-layer runway pool reactor equipment comprises a runway pool, a stirring paddle wheel and a gas supply system, wherein the stirring paddle wheel is a cross paddle wheel made of stainless steel; the gas supply system is a rubber membrane aerator and a connecting pipeline thereof, the aperture of the aerator is 0.8mm, and the porosity is 0.85. When the incident sunlight vertically irradiates the reactor system, the light is sequentially absorbed and utilized by the upper horizontal transverse pipe and the lower raceway pond, and light and dark areas are formed in the flowing direction of the algae liquid on the surface of the raceway pond, so that a flash effect is formed when the microalgae cells in the raceway pond flow, and the growth of the microalgae cells is promoted.
The method for realizing microalgae growth carbon sequestration by using the tube-pool combined flash bioreactor system comprises the following steps: inoculating microalgae culture solution (Chlorella) into CO of tube-pool combined flash bioreactor system2In a dissolver, culturing microalgaeThe inoculation mass ratio of the nutrient solution to the water body in the whole pipe-pool combined reactor is 7%. Introducing CO into microalgae culture solution2And starting the centrifugal pump after the dissolver, pumping the algae solution into the upper-layer multi-group horizontal transverse tubular reactors for photosynthesis growth, and then sending the algae solution into the lower-layer raceway pond for photosynthesis growth. CO in industrial flue gas2(derived from flue gas, CO, discharged from industrial furnaces230% by volume) through CO2A gas distributor inside the dissolver is sent into an upper horizontal transverse pipe and simultaneously sends industrial flue gas CO2Feeding the industrial flue gas into a runway pool through a membrane aerator to control the CO of the industrial flue gas2The aeration rate of the flow rate compared to the water in the entire tube-pool combined reactor was 0.04 vvm. The illumination intensity of incident sunlight is 30000Lux, and the ambient temperature is 25 ℃. The algae liquid circularly flows between the upper horizontal transverse pipe and the lower runway pool, and the biomass is harvested after 4 days of growth by photosynthesis.
Compared with the traditional simple raceway pond reactor (no horizontal pipe is arranged above the reactor), the tube-pond combined flash bioreactor system is adopted to improve the flash frequency of microalgae cell movement by 23.9%, the photochemical efficiency (Fv/Fm) of a photosystem II (PS II) by 8.6%, the contents of chlorophyll a and chlorophyll b in algae cells by 14.3% and 8.9%, respectively, the growth rate of microalgae by 29.2% and the yield of microalgae biomass in unit floor area by 32.7%.
Example 3
The tube-pool combined flash bioreactor system is formed by combining an upper horizontal transverse tube with a lower runway pool, and the horizontal transverse tube and the runway pool are in a cross shape. 1000 rows of horizontal transverse pipes are arranged above the length direction of the runway pool, and 10 runway pools are arranged below the horizontal transverse pipes in the length direction. The distance between the bottom of the horizontal transverse pipe and the bottom of the runway pool is 2 meters. The upper horizontal transverse pipe is vertically stacked to form a multi-layer transverse pipe structure, the pipe diameter of the upper horizontal transverse pipe is 5 cm, and the upper horizontal transverse pipe is made of light-transmitting pipes (namely tempered glass with light transmittance of more than 80%). The total number of layers of the horizontal transverse pipes is 5, the pipe wall distance between every two adjacent pipes in the vertical direction is 5 times of the pipe diameter, and the pipe wall distance between every two adjacent pipes in the horizontal direction is 1 time of the pipe diameter. The upper horizontal cross-tube reactor is provided withIs prepared from CO2Dissolver, multiple groups of horizontal transverse pipes and algae liquid circulating system, wherein the dissolver is made of CO made of stainless steel material2The dissolver is a gas-liquid mixing device, and the gas distributor inside the dissolver is connected to CO through a pipeline2The air source is used for inoculating the algae liquid to the inlet of the horizontal tube; the algae liquid circulating system comprises a centrifugal pump and a connecting pipeline, and the horizontal transverse pipe and the CO are connected by the connecting pipeline2The dissolver is connected with the dissolver in a sealing way and is used for driving the algae liquid to be connected with CO in the horizontal transverse pipe2Circulating flow in the dissolver. The lower-layer runway pool reactor equipment comprises a runway pool, a stirring paddle wheel and a gas supply system, wherein the stirring paddle wheel is a cross paddle wheel made of stainless steel; the gas supply system is a rubber membrane aerator and a connecting pipeline thereof, the aperture of the aerator is 0.8mm, and the porosity is 0.85. When the incident sunlight vertically irradiates the reactor system, the light is sequentially absorbed and utilized by the upper horizontal transverse pipe and the lower raceway pond, and light and dark areas are formed in the flowing direction of the algae liquid on the surface of the raceway pond, so that a flash effect is formed when the microalgae cells in the raceway pond flow, and the growth of the microalgae cells is promoted.
The method for realizing microalgae growth carbon sequestration by using the tube-pool combined flash bioreactor system comprises the following steps: inoculating microalgae culture solution (Spirulina as strain) into CO of tube-pool combined flash bioreactor system2In the dissolver, the inoculation mass ratio of the microalgae culture solution to the water body in the whole tube-pool combined reactor is 10%. Introducing CO into microalgae culture solution2And starting the centrifugal pump after the dissolver, pumping the algae solution into the upper-layer multi-group horizontal transverse tubular reactors for photosynthesis growth, and then sending the algae solution into the lower-layer raceway pond for photosynthesis growth. CO in industrial flue gas2(derived from flue gas, CO, discharged from coal chemical plants299% by volume) through CO2A gas distributor inside the dissolver is sent into an upper horizontal transverse pipe and simultaneously sends industrial flue gas CO2Feeding the industrial flue gas into a runway pool through a membrane aerator to control the CO of the industrial flue gas2The aeration rate of the flow rate compared to the water in the entire tube-pool combined reactor was 0.1 vvm. The illumination intensity of incident sunlight is 100000Lux, and the ambient temperature is 38 ℃. The algae liquid circulates between the upper horizontal pipe and the lower runway poolAnd (4) flowing, and harvesting the biomass after 5 days of growth by photosynthesis.
Compared with the traditional simple raceway pond reactor (no horizontal pipe is arranged above the reactor), the tube-pond combined flash bioreactor system is adopted to improve the flash frequency of microalgae cell movement by 34.2%, the photochemical efficiency (Fv/Fm) of a photosystem II (PS II) by 12.1%, the contents of algae cell chlorophyll a and chlorophyll b are respectively improved by 21.1% and 11.2%, the growth rate of microalgae is improved by 36.9%, and the yield of microalgae biomass in unit floor area is improved by 41.2%.
Finally, it should be noted that the above-mentioned embodiments illustrate only specific embodiments of the invention. Obviously, the present invention is not limited to the above embodiments, and many variations are possible. All modifications which can be derived or suggested by a person skilled in the art from the disclosure of the invention should be considered as within the scope of the invention.
Claims (6)
1. A tube-pool combined flash bioreactor system comprises a raceway pool reactor for culturing microalgae; the device is characterized in that a plurality of horizontal transverse pipes made of light-transmitting materials are arranged above the raceway pond reactor in parallel, and the horizontal transverse pipes are vertical to the length direction of the raceway pond reactor; the end parts of the adjacent horizontal transverse pipes are connected by a bent pipe to form a through horizontal transverse pipe reactor; the outlet end of the horizontal transverse tube reactor is sequentially connected with the raceway pond reactor and CO through a pipeline2Dissolver, CO2The outlet end of the dissolver is sequentially connected with the centrifugal pump and the inlet end of the horizontal transverse tube reactor through pipelines; in CO2The dissolver is provided with a gas distributor which is connected to the CO through a pipeline2And (4) a gas source.
2. The system according to claim 1, wherein the horizontal transverse pipes are divided into 1-5 layers and are arranged in an overlapped mode in the vertical direction, and the number of the transverse pipes in each layer is 10-1000 rows; the pipe wall space between two adjacent pipes in the vertical direction is 1-5 times of the pipe diameter, and the pipe wall space between two adjacent pipes in the horizontal direction is 1-10 times of the pipe diameter.
3. The system of claim 1, wherein the number of raceway pond reactors is 3 to 10; the runway pool reactors are arranged in a mode that the length directions are mutually parallel and are sequentially connected end to end; each raceway pond reactor is internally provided with a stirring paddle wheel and a membrane aerator.
4. The system of claim 3, wherein the paddle wheel is a cross paddle wheel of stainless steel; the membrane aerator is made of rubber, the aperture is 0.8mm, and the porosity is 0.85; the membrane aerator is connected with CO through a pipeline2And (4) a gas source.
5. The system of claim 1, wherein the diameter of the horizontal cross tube is 5 cm, and the minimum distance between the bottom of the horizontal cross tube and the bottom of the runway pool is 2 m.
6. The system of claim 1, wherein the horizontal cross tube is a common glass tube, a tempered glass tube or a polymer plastic tube having a light transmittance of 80% or more.
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