CN103525686B - Composite reinforced microalgae photobioreactor based on hollow light pipes - Google Patents
Composite reinforced microalgae photobioreactor based on hollow light pipes Download PDFInfo
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Abstract
The invention discloses a composite reinforced microalgae photobioreactor based on hollow light pipes. The photobioreactor comprises a reactor roof, stainless steel coiled pipes, the hollow light pipes, a gas distributor, a reactor bottom plate, a reactor main body, a gas-liquid separation device and an online pH, temperature and DO (dissolved oxygen) monitoring device, wherein the stainless steel coiled pipes are arranged at left and right inside the reactor main body, respectively; the gas distributor is an annular pipe penetrating through the bottom in the reactor main body, and a plurality of air outlets are formed in the gas distributor inside the reactor main body; a plurality of hollow light pipes are uniformly arranged in the reactor main body; the gas-liquid separation device is arranged on the reactor roof; the online pH, temperature and DO monitoring device is arranged inside the reactor main body. The reactor has the main advantage of coupling reinforcing of light transfer and CO2 mass transfer and reinforcing of a microalgae cell photo-biochemical reaction together, so that the comprehensive performance of the microalgae photobioreactor can be improved.
Description
Technical field
The present invention relates to a kind of bioreactor for photosynthetic microdisk electrode, particularly relate to a kind of complex intensifying based on hollow light guiding pipe and to decline algae optical biological reactor.
Background technology
Global warming and fossil energy shortage are two hang-ups that the mankind faced in 21st century.Global warming mainly causes due to a large amount of discharges of greenhouse gases, greenhouse gases are of a great variety and different types of greenhouse gases are different to the contribution of Greenhouse effect, carbonic acid gas is considered to topmost greenhouse gases, it is maximum to the contribution of Greenhouse effect, and the discharge therefore reducing carbonic acid gas effectively can alleviate the trend of global warming.The shortage of fossil energy is mainly due to caused to consuming excessively of fossil energy in human social development process, and the Devoting Major Efforts To Developing of renewable energy source effectively can alleviate the restrictive function of fossil energy shortage to human social development.Photosynthetic micro-algae both may be used for the discharge that high-performance bio stabilizing carbon dioxide reduces greenhouse gases, simultaneously ripe microalgae biomass may be used for again a difficult problem for production biofuel reply fossil energy shortage, now become the focus of international community's research, start the upsurge that one studies micro-algae.
Micro-algae is that a class not only can photoautotrophy but also can the microorganism of heterotrophism; it is of a great variety; widely distributed; wherein the micro-algae of photosynthetic autotrophs has the advantages such as photosynthetic rate is high, strong, the ripe microalgae biomass of fast growth, adaptive capacity to environment is of many uses; can be used for efficient stabilizing carbon dioxide and reduce greenhouse gas emission; simultaneously; ripe microalgae biomass both can be used for production biofuel alleviating energy crisis; can be used for again producing the high value added products such as medicine, makeup, healthcare products, there is the good energy and economic worth.
Bioreactor is the product institute that photosynthetic micro-algae carries out photosynthesis stabilizing carbon dioxide synthesis of organic substance.Efficient bioreactor is conducive to the high-density, the large scale culturing that realize micro-algae.The bioreactor that present stage is used for microdisk electrode is mainly divided into open and closed two kinds, wherein Race-way photobioreactor mainly refers to raceway pond bioreactor, it has, and laid down cost is low, simple operation and other advantages, but its culture condition is wayward, microalgae biomass yields poorly, algae kind vulnerable to pollution, the cultivation that chlorella, spirulina and salt algae etc. can tolerate the algae of extreme environment can only be used for.Closed photo bioreactor overcomes the above-mentioned shortcoming of Race-way photobioreactor, have culture condition controlled, be applicable to the advantages such as all algae culture, the high-density that can realize microalgae cell and large scale culturing, but the shortcomings such as cost is high, complicated operation that it has, but consider the utility value that micro-algae is good, the focus that closed photo bioreactor will be research all the time.Therefore, a kind of closed photo bioreactor that is efficient, low cost is designed most important for the large-scale application realizing micro-algae.
Luminous energy and carbon source are the two large principal elements affecting photosynthetic autotrophs micro algae growth, and therefore, efficient microalgae photobiological reactor should be able to provide good luminous energy supply and carbon source supply.
First, luminous energy is that micro-algae carries out photosynthesis institute essential condition, but for the growth of micro-algae, light intensity can produce photoinhibition too by force, light intensity is too weak, can produce light restriction phenomenon, light restriction and Xanthophyll cycle are all unfavorable for the growth of micro-algae, and the light distribution therefore in bioreactor should avoid the generation of light restriction and photoinhibition as far as possible.On the other hand, along with the growth of microalgae cell, in reactor, micro algae biomass concentration rises, along optical propagation direction in algae liquid, light intensity will exponentially rule decay, make the region light intensity away from light source place in reactor very weak, the demand of micro algae growth to luminous energy can not be met, therefore, efficient bioreactor should be able to provide larger light application ratio surface-area, region light intensity away from light source place in reactor is too weak, to such an extent as to the demand of micro algae growth to light can not be met, efficient microalgae photobiological reactor should be able to import light into the region that in reactor, optical attenuation is serious, improve the efficiency of light energy utilization.
Secondly, for most of bioreactor, carbonic acid gas is the carbon source of micro algae growth, carbonic acid gas mainly to enter in the micro-algae suspension in reactor with the form of bubble via the gas distributor in reactor, the size of bubble will affect total gas liquid interfacial area, and then affects mass transfer effect.Bubble is less, and its specific surface area is larger, and gas-liquid mass transfer is better.On the other hand, when in the process that bubble floats after reactor bottom produces in micro-algae suspension, easily there is coalescence phenomenon in the bubble of consecutive position, and make bubble volume become large, specific surface area reduces, and makes gas-liquid mass transfer deleterious.Therefore, efficient bioreactor should have good gas distributor and produce evenly tiny bubble, certain measure also should be taked to avoid or reduce the coalescence phenomenon in bubble floating process simultaneously, and then strengthening gas-liquid mass transfer.
Moreover, there are some researches show, when the intermittent alternating motion of microalgae cell is in reactor when light intensity region (light district) and light weak-strong test (dark space), its growth velocity and optical energy utilization efficiency can be greatly improved, people claim this effect for " sparkle effect ", namely, when frustule shuttles between the light district and dark space of bioreactor, the microalgae cell having accepted illumination just can be made to enter dark space in time and to carry out dark reaction, simultaneously, make the microalgae cell completing dark reaction get back to light district and again accept illumination, the photon entered in reactor so just can be made to be fully utilized.Therefore, when designing high-efficiency photobioreactor, can by modes such as the structures of altering reactor, make frustule alternating motion Yu Guangqu and dark space, the motion of frustule meets certain light/dark loop cycle, realizes the object that " sparkle effect " significantly improves microalgae biomass productive rate and the efficiency of light energy utilization.
Good disturbance is also vital for the growth of micro-algae, its advantage be mainly reflected in following some: 1. good disturbance can strengthen gas-liquid mass transfer.The mass transfer of strengthening carbon source from gas phase zone to liquid phase region on the one hand, growth for frustule provides sufficient carbon source, reinforcing mass transfer is conducive to realizing discharging dissolved oxygen in time from microalgae cell suspension on the other hand, avoids the too high restraining effect to algal grown of oxyty; 2. good disturbance can reduce the intercellular deposition with microalgae cell of mutually blocking, and is conducive to ensureing that each frustule enjoys identical intensity of illumination and nutritive substance condition; 3. good disturbance can avoid the thermal stratification in microalgae cell suspension; 4. good disturbance can make frustule replace the light intensity region of back and forth movement in reactor and light weak-strong test, is conducive to utilizing light/dark Alternate Phenomenon strengthening light biochemical reaction, improves micro algae growth speed and the efficiency of light energy utilization.
To sum up, the design of efficient microalgae photobiological reactor can be considered from following three angles: the transmission 1. strengthening light, increases light application ratio surface-area, improves the efficiency of light energy utilization; 2. reinforcing mass transfer, the growth for frustule provides sufficient carbon source, and discharges the oxygen of photosynthesis generation in time; 3. light biochemical reaction is strengthened, for the light biochemical reaction in microalgae cell provides optimal conditions.
Therefore, if above-mentioned three strengthening means can be coupled, the over-all properties improving microalgae photobiological reactor will be conducive to.
Summary of the invention
For above shortcomings in prior art, the invention provides a kind of strengthen light transmission, reinforcing mass transfer and strengthening light biochemical reaction the complex intensifying based on hollow light guiding pipe to decline algae optical biological reactor.
In order to solve the problems of the technologies described above, present invention employs following technical scheme:
Complex intensifying based on hollow light guiding pipe declines an algae optical biological reactor, comprises reactor top board, stainless steel serpentine tube, hollow light guiding pipe, gas distributor, fluorescent light, reactor base plate, reactor body, gas-liquid separation device and pH, temperature, DO on-Line Monitor Device;
Described reactor top board, reactor body and reactor base plate are made by light transmissive material;
Described reactor body is the rectangular box of upper and lower both ends open, and described reactor top board covers the upper port at reactor body and is sealed and matched with upper port, and described reactor base plate is arranged on the lower port of reactor body and is sealed and matched with lower port; The top side wall of described reactor body is provided with opening for feed, and sidewall bottom is provided with discharge port, and the sidewall of described reactor body is also provided with thief hole;
The left and right sides in described reactor body arranges stainless steel serpentine tube respectively, and the two-port of described stainless steel serpentine tube all stretches out reactor top board, and wherein Single port is constant temperature circulating water inlet, and another port is constant temperature circulating water out;
Described gas distributor is a ring pipe passing reactor body inner bottom part, a part for this ring pipe is positioned at reactor body, the remainder of ring pipe is positioned at the outside of reactor body, the sidewall of ring pipe and reactor body is sealed and matched, and described ring pipe is positioned at that the part of reactor body is uniform arranges multiple production well; The outside that described ring pipe is positioned at reactor body is provided with CO
2gas inlet;
Described reactor body is uniform arranges multiple hollow light guiding pipe, described hollow light guiding pipe is horizontally disposed with, the one end open of hollow light guiding pipe, the other end is blind end, the opening end of hollow light guiding pipe to be arranged on the front side wall in reactor body and to embed front side wall, cylindrical and the front side wall of the opening end of hollow light guiding pipe are sealed and matched, and the blind end of hollow light guiding pipe to be arranged on the rear wall in reactor body and to be sealed and matched with rear wall; The opening end of described hollow light guiding pipe is all coated with total reflection film to the inwall of distance opening end 3cm and on the inwall of blind end; Described fluorescent light is arranged on the outside of the front side wall of reactor body;
Described gas-liquid separation device is arranged on reactor top board, and the import of gas-liquid separation device is communicated with in reactor body by pipeline, gas-liquid separation device has a reactor venting port; Described pH, temperature, DO on-Line Monitor Device are arranged in reactor body.
As a preferred embodiment of the present invention, described reactor top board is sealed and matched by the upper port of silica gel pad and reactor body.
As another kind of preferred version of the present invention, described reactor base plate is sealed and matched by the lower port of silica gel pad and reactor body.
As a modification of the present invention scheme, the aperture of the production well on described gas distributor is 1mm, and the distance between adjacent two production wells is 15mm.
The invention has the beneficial effects as follows: this reactor, is mainly reflected in the strengthening of light transmission, CO by the Combination of Methods of multiple enhanced reactor performance together
2the strengthening of mass transfer and the strengthening of microalgae cell light biochemical reaction are coupled, but not adopt single mode to strengthen, and improve the over-all properties of microalgae photobiological reactor.
Accompanying drawing explanation
Fig. 1 is that a kind of complex intensifying based on hollow light guiding pipe declines the structural representation of algae optical biological reactor;
Fig. 2 is be flat bioreactor local section structural representation bottom hollow light guiding pipe;
Fig. 3 is be hemispheric bioreactor local section structural representation bottom hollow light guiding pipe;
The structural representation of the microalgae photobiological reactor of Fig. 4 to be hollow light guiding pipe arrangement mode be fork row;
The structural representation of Fig. 5 to be hollow light guiding pipe arrangement mode be microalgae photobiological reactor of in-line arrangement.
In accompanying drawing: 1-reactor top board; 2-silica gel pad; 3-stainless steel serpentine tube; 4-hollow light guiding pipe; 5-gas distributor; 6-reactor base plate; 7-CO
2gas inlet; 8-discharge port; 9-total reflection film; 10-thief hole; 11-reactor body; 12-opening for feed; 13-bolt; 14-pH, temperature, DO on-Line Monitor Device; 15-reactor venting port; 16-gas-liquid separation device; 17-constant temperature circulating water out; 18-constant temperature circulating water inlet; 19-fluorescent light; 20-incident beam; 21-air; 22-reactor body rear wall; 23-microalgae cell suspension; 24 — Guang ∕ dark areas line of delimitation; 25-reactor body front side wall; 26-region two; 27-region one.
Embodiment
Below in conjunction with the drawings and specific embodiments, the present invention is described in further detail.
As shown in Figure 1, complex intensifying based on hollow light guiding pipe declines an algae optical biological reactor, comprises reactor top board 1, stainless steel serpentine tube 3, hollow light guiding pipe 4, gas distributor 5, reactor base plate 6, reactor body 11, fluorescent light 19, gas-liquid separation device 16 and pH, temperature, DO on-Line Monitor Device 14.
Reactor top board 1, reactor body 11 and reactor base plate 6 are made by light transmissive material, form as adopted synthetic glass or other materials processing with excellent light transmission.Reactor body 11 is the rectangular box of upper and lower both ends open, reactor top board 1 is covered the upper port at reactor body 11 and is connected by bolt 13, silica gel pad 2 is set between reactor top board 1 and the upper port of reactor body 11, by being sealed and matched between this silica gel pad 2 realization response device top board 1 and upper port of reactor body 11.Reactor base plate 6 is arranged on the lower port of reactor body 11 and is connected by bolt 13, silica gel pad 2 is set between reactor base plate 6 and the lower port of reactor body 11, by being sealed and matched between this silica gel pad 2 realization response device base plate 6 and lower port of reactor body 11.All be lined with silica gel pad 2 between reactor top board 1 and reactor body 11 and between reactor base plate 6 and reactor body 11, mainly prevent the leakage of algae liquid.The top side wall of reactor body 11 is provided with opening for feed 12, sidewall bottom is provided with discharge port 8, opening for feed 12 for adding certain density microalgae cell suspension during initial incubation in reactor body 11, the collection to microalgae cell when discharge port 8 is in stationary phase for microalgae cell growth.The sidewall of reactor body 11 is also provided with thief hole 10, and thief hole 10 carries out microalgae cell biomass, chlorophyll content, fat content and composition, the isoparametric measurement of protein content for regularly sampling from the microalgae cell suspension in reactor body 11.
The left and right sides in reactor body 11 arranges stainless steel serpentine tube 3 respectively, and the two-port of stainless steel serpentine tube 3 all stretches out reactor top board 1, and wherein Single port is constant temperature circulating water inlet 18, and another port is constant temperature circulating water out 17.Stainless steel serpentine tube 3 internal circulation flow the circulation constant temperature water of homo(io)thermism, and the microalgae cell suspension in the recirculated water of homo(io)thermism and reactor body 11 carries out heat exchange, for maintaining the constant of microalgae cell suspension temperature.
Gas distributor 5 is a ring pipe passing reactor body 11 inner bottom part, a part for this ring pipe is positioned at reactor body 11, the remainder of ring pipe is positioned at the outside of reactor body 11, the sidewall of ring pipe and reactor body 11 is sealed and matched, and ring pipe is positioned at that the part of reactor body 11 is uniform arranges multiple production well; The outside that ring pipe is positioned at reactor body 11 is provided with CO
2gas inlet 7.The middle position of gas distributor 5 between reactor body 11 front-rear side walls, gas distributor 5 is made by materials such as PU pipe, pvc pipe, stainless steel tubes, production well is uniformly distributed along pipe axial direction due, and aperture is 1mm, and the width between centers between adjacent holes is 15mm.In order to ensure that gas distributor internal gas pressure is axially evenly distributed along pipe, adopting the mode of gas distributor 5 two ends air inlet simultaneously to realize intraductal atmospheric pressure and being axially uniformly distributed along pipe, make the Air Bubble Size via gas distributor generation more even.
Reactor body 11 is uniform arranges multiple hollow light guiding pipe 4, hollow light guiding pipe 4 is horizontally disposed with, the one end open of hollow light guiding pipe 4, the other end is blind end, the opening end of hollow light guiding pipe 4 to be arranged on the front side wall in reactor body 11 and to embed front side wall, cylindrical and the front side wall of the opening end of hollow light guiding pipe 4 are sealed and matched, and the blind end of hollow light guiding pipe 4 to be arranged on the rear wall in reactor body 11 and to be sealed and matched with rear wall.The opening end of hollow light guiding pipe 4 is all coated with total reflection film 9 to the inwall of distance opening end 3cm and on the inwall of blind end, and fluorescent light 19 is arranged on the outside of the front side wall of reactor body 11.Inside is coated with the hollow light guiding pipe 4 of total reflection film 9 can by silica tube, the materials processing that plexi-glass tubular etc. have excellent light transmission forms, flat (as shown in Figure 2) or semi-spherical shape (as shown in Figure 3) can be made in the bottom of hollow light guiding pipe 4, the entrance (i.e. the opening end of hollow light guiding pipe 4) of hollow light guiding pipe 4 and bottom (blind end of hollow light guiding pipe 4) are coated with the total reflection film 9 of high-reflectivity, the external world injects the light of hollow light guiding pipe 4 under the effect of total reflection film 9, the region (i.e. light restricted areas 26) serious by optical attenuation in the postmedian of hollow light guiding pipe 4 and frustule suspension is penetrated, for the growth of microalgae cell, as Fig. 2, shown in 3, region I 27 is the region near incident light source, light intensity can meet the region of the photosynthetic growth of micro-algae, region II 26 is the region away from incident light source, optical attenuation critical regions, and light intensity is lower, i.e. light restricted area.Hollow light guiding pipe 4 is arranged between the front-rear side walls of reactor body 11, perpendicular with the front-rear side walls of reactor body 11, and the arrangement mode of hollow light guiding pipe 4 can be divided into in-line arrangement and fork row two kinds, as shown in Figure 4,5.
The effect of this hollow light guiding pipe 4 be mainly reflected in following some: one, for the incident light in the external world is imported the region that in reactor body 11, optical attenuation is serious, increase light application ratio surface-area, improve the efficiency of light energy utilization; Two, the existence of hollow light guiding pipe 4 can flow field in altering reactor main body 11, the mixing in enhanced reactor, is conducive to reinforcing mass transfer, the mixed effect optimized in reactor; Three, the arranged direction due to hollow light guiding pipe 4 is perpendicular with the direction of motion of the bubble floating process produced via gas distributor 5, hollow light guiding pipe 4 can smash the bubble of floating to a certain extent, increase the specific surface area of bubble, reduce the generation of coalescence phenomenon in bubble floating process; Four, because hollow light guiding pipe 4 imports light into the serious region of light intensity attenuation in reactor, the light intensity of hollow light guiding pipe 4 bottom periphery is made to want large compared with the light intensity of its peripheral region, the frustule moved up and down like this will alternating motion in the different region of light intensity, namely the motion of frustule meets certain light/dark cycle, is conducive to improving microalgae biomass productive rate by " sparkle effect ".
Gas-liquid separation device 16 is arranged on reactor top board 1, the import of gas-liquid separation device 16 is communicated with in reactor body 11 by pipeline, gas-liquid separation device 16 has a reactor venting port 15, gas-liquid separation device 16 is for the separation of gas-liquid two-phase, for getting rid of the oxygen produced in frustule suspension, undissolved carbonic acid gas and other undissolved gas, the moisture carried in gas recovery, reduces scattering and disappearing of moisture.PH, temperature, DO on-Line Monitor Device 14 are arranged in reactor body 11, and pH, temperature, DO on-Line Monitor Device 14 are for pH value, temperature, the DO changing conditions of microalgae cell suspension in real time on-line monitoring reactor.
The principle of work of this reactor: first the microalgae cell suspension of certain starting point concentration is placed in microalgae photobiological reactor, there is the CO of certain volume concentration
2gas is (as CO
2volumetric concentration is air and the CO of 5%
2mixed gas) by CO
2gas inlet 7 passes into gas distributor 5, is rich in CO
2gas mixture enter microalgae cell suspension via the production well that diameter equally distributed on gas distributor 5 is 1mm with the form of small bubbles, small bubbles are moved to the top of reactor body 11 under the effect of buoyancy by the bottom of reactor body 11 subsequently, in the process floated, because hollow light guiding pipe 4 axial direction due inserted in bubble motion direction and reactor body 11 is perpendicular, some bubbles are smashed by hollow light guiding pipe 4 in the process floated, become the small bubbles that volume is less, these bubbles finally depart from from microalgae cell suspension, air is entered via reactor venting port 15, be rich in CO
2small bubbles float process in, CO
2reach liquid phase region by gas phase zone, and finally reach in frustule for growth.Light enters microalgae cell suspension by the front side wall of reactor body 11, for the growth of microalgae cell provides luminous energy, due to light intensity exponentially rule decay in algae liquid, make the region light intensity decreasing away from front side wall, the hollow light guiding pipe 4 of total reflection film 9 is coated with owing to adding inside in reactor, the entrance of hollow light guiding pipe 4 and bottom are coated with the total reflection film of high-reflectivity, and the decrement of light when propagating in atmosphere is more much lower than the decrement in algae liquid, stronger light is made to penetrate away from the region (light restricted area) of front side wall from the afterbody and reactor of hollow light guiding pipe 4 like this, achieve the target of luminous energy lead-in light decay critical regions.The introducing of hollow light guiding pipe 4, serves the effect of smashing bubble enhancing mixed on the one hand; On the other hand, import light into the region that optical attenuation is serious, add light application ratio surface-area, improve the efficiency of light energy utilization.
Use this complex intensifying based on hollow light guiding pipe decline algae optical biological reactor time, first sterilizing 30min is carried out by formaldehyde solution by inside reactor and gas distributor tube, then the distilled water after autoclaving is utilized to clean reactor, during inoculation, the microalgae cell suspension of initial low concentrations (if frustule biomass concentration is 0.05g/L) is pumped in reactor by opening for feed 12.Two stainless steel serpentine tubes 3 are distributed in the region of close reactor body about 11 sidewall in reactor body 11, temperature is that the thermostat(t)ed water of 25 DEG C enters stainless steel serpentine tube 3 with certain flow velocity by constant temperature circulating water inlet 18 under the driving of peristaltic pump, after flowed out by constant temperature circulating water out 17, enter constant temperature water bath, complete circulating of thermostat(t)ed water, thermostat(t)ed water is for maintaining the constant of microalgae cell suspension temperature, and the temperature keeping microalgae cell suspension is 25 DEG C.Light source arrangement mode, adopts one-sided lighting, and the fluorescent tube bulb of 4 8W is parallel to the front of reactor body front side wall, for the photosynthesis of micro-algae provides light source.Ingress to the distance 3cm position, ingress of hollow light guiding pipe 4 and the flat or hemispherical bottom of hollow light guiding pipe 4 are all coated with the total reflection film 9 of high-reflectivity, and ambient light can be imported the serious region of optical attenuation in reactor by such hollow light guiding pipe 4 one aspect.Increase illuminating surface to amass, improve the efficiency of light energy utilization; On the other hand, the arranged direction of open tube is perpendicular to CO
2the direction of air bubble buoyancy motion, opens and smashes bubble, reduces the mutual coalescence between bubble, increases flow-disturbing, the effect of strengthening gas-liquid mass transfer.The parameter such as biomass, chlorophyll, fat content detecting microalgae cell is regularly sampled from thief hole 10, the growing state of monitoring frustule, when frustule grows to stationary phase, frustule suspension is released from discharge port 8, be used after collection, as the production etc. for the producing of biofuel, high value added product.
What finally illustrate is, above embodiment is only in order to illustrate technical scheme of the present invention and unrestricted, although with reference to preferred embodiment to invention has been detailed description, those of ordinary skill in the art is to be understood that, can modify to technical scheme of the present invention or equivalent replacement, and not departing from aim and the scope of technical solution of the present invention, it all should be encompassed in the middle of right of the present invention.
Claims (4)
1. to decline an algae optical biological reactor based on the complex intensifying of hollow light guiding pipe, it is characterized in that: comprise reactor top board (1), stainless steel serpentine tube (3), hollow light guiding pipe (4), gas distributor (5), reactor base plate (6), reactor body (11), fluorescent light (19), gas-liquid separation device (16) and pH, temperature, DO on-Line Monitor Device (14);
Described reactor top board (1), reactor body (11) and reactor base plate (6) are made by light transmissive material;
The rectangular box that described reactor body (11) is upper and lower both ends open, described reactor top board (1) is covered the upper port at reactor body (11) and is sealed and matched with upper port, and described reactor base plate (6) is arranged on the lower port of reactor body (11) and is sealed and matched with lower port; The top side wall of described reactor body (11) is provided with opening for feed (12), and sidewall bottom is provided with discharge port (8), the sidewall of described reactor body (11) is also provided with thief hole (10);
The left and right sides in described reactor body (11) arranges stainless steel serpentine tube (3) respectively, the two-port of described stainless steel serpentine tube (3) all stretches out reactor top board (1), wherein Single port is constant temperature circulating water inlet (18), and another port is constant temperature circulating water out (17);
Described gas distributor (5) is a ring pipe passing reactor body (11) inner bottom part, a part for this ring pipe is positioned at reactor body (11), the remainder of ring pipe is positioned at the outside of reactor body (11), the sidewall of ring pipe and reactor body (11) is sealed and matched, and described ring pipe is positioned at that the part of reactor body (11) is uniform arranges multiple production well; The outside that described ring pipe is positioned at reactor body (11) is provided with CO
2gas inlet (7);
Described reactor body (11) is uniform arranges multiple hollow light guiding pipe (4), described hollow light guiding pipe (4) is horizontally disposed with, the one end open of hollow light guiding pipe (4), the other end is blind end, the opening end of hollow light guiding pipe (4) to be arranged on the front side wall in reactor body (11) and to embed front side wall, cylindrical and the front side wall of the opening end of hollow light guiding pipe (4) are sealed and matched, and the blind end of hollow light guiding pipe (4) to be arranged on the rear wall in reactor body (11) and to be sealed and matched with rear wall; The opening end of described hollow light guiding pipe (4) is all coated with total reflection film (9) to the inwall of distance opening end 3cm and on the inwall of blind end; Described fluorescent light (19) is arranged on the outside of the front side wall of reactor body (11);
Described gas-liquid separation device (16) is arranged on reactor top board (1), and the import of gas-liquid separation device (16) is communicated with in reactor body (11) by pipeline, gas-liquid separation device (16) has a reactor venting port (15); Described pH, temperature, DO on-Line Monitor Device (14) are arranged in reactor body (11).
2. a kind of complex intensifying based on hollow light guiding pipe according to claim 1 declines algae optical biological reactor, it is characterized in that: described reactor top board (1) is sealed and matched by the upper port of silica gel pad (2) with reactor body (11).
3. a kind of complex intensifying based on hollow light guiding pipe according to claim 1 declines algae optical biological reactor, it is characterized in that: described reactor base plate (6) is sealed and matched by the lower port of silica gel pad (2) with reactor body (11).
4. a kind of complex intensifying based on hollow light guiding pipe any one of claims 1 to 3 described in claim declines algae optical biological reactor, it is characterized in that: the aperture of the production well on described gas distributor (5) is 1mm, the distance between adjacent two production wells is 15mm.
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| CN104017726A (en) * | 2014-06-30 | 2014-09-03 | 张玟籍 | Built-in light source bioreactor and microalgae culture method |
| CN104312901A (en) * | 2014-10-20 | 2015-01-28 | 杭州鑫伟低碳技术研发有限公司 | Microalgae cultivation module and large-scale microalgae factory cultivation system |
| CN105039134B (en) * | 2015-05-28 | 2017-08-25 | 连衡会投资有限公司 | Recycle stream dynamic formula photo-bioreactor system |
| CN104928152B (en) * | 2015-06-24 | 2017-06-09 | 新奥科技发展有限公司 | A kind of bioreactor |
| CN108485936B (en) * | 2018-05-15 | 2024-03-29 | 中国科学技术大学 | Microalgae experimental device |
| CN112280652A (en) * | 2020-10-27 | 2021-01-29 | 南京师范大学镇江创新发展研究院 | Flowing type microalgae photobioreactor based on light energy gradient utilization |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101709262A (en) * | 2009-12-10 | 2010-05-19 | 中国科学院广州能源研究所 | Solar spectral photosynthetic bioreactor system for culturing microalgae in high density |
| CN102382754A (en) * | 2010-09-03 | 2012-03-21 | 中国科学院过程工程研究所 | Optical fiber photobioreactor for making full use of 'flash effect' of microalgae |
| CN103205361A (en) * | 2013-04-22 | 2013-07-17 | 青岛中仁药业有限公司 | Aerosol microalgae illuminating reaction device |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101709262A (en) * | 2009-12-10 | 2010-05-19 | 中国科学院广州能源研究所 | Solar spectral photosynthetic bioreactor system for culturing microalgae in high density |
| CN102382754A (en) * | 2010-09-03 | 2012-03-21 | 中国科学院过程工程研究所 | Optical fiber photobioreactor for making full use of 'flash effect' of microalgae |
| CN103205361A (en) * | 2013-04-22 | 2013-07-17 | 青岛中仁药业有限公司 | Aerosol microalgae illuminating reaction device |
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