CN104928152B - A kind of bioreactor - Google Patents
A kind of bioreactor Download PDFInfo
- Publication number
- CN104928152B CN104928152B CN201510357644.0A CN201510357644A CN104928152B CN 104928152 B CN104928152 B CN 104928152B CN 201510357644 A CN201510357644 A CN 201510357644A CN 104928152 B CN104928152 B CN 104928152B
- Authority
- CN
- China
- Prior art keywords
- bioreactor
- pipe
- feeder
- aeration tube
- smooth surface
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
Abstract
The invention discloses a kind of bioreactor, it is related to photo-biological cultural technique field, the cultured output of photo-biological can be improved.The bioreactor includes hollow reactor body, the reactor body has at least one smooth surface, the bioreactor also includes the aerator in the reactor body, and the aerator is used for the direction jet towards the smooth surface.Bioreactor in the present invention is used to cultivate photo-biological.
Description
Technical field
The present invention relates to photo-biological cultural technique field, more particularly to a kind of bioreactor.
Background technology
Photo-biological is that one kind can carry out photosynthetic photoautotroph, and photo-biological is converted light energy into by photosynthesis
Chemical energy so that photo-biological has in all many-sides such as bioenergy, biological sequestration, environmental protection, food, feed and medicine extensively should
With.At present, it is main photo-biological is largely cultivated using bioreactor, in breeding process, photo-biological is mixed into light
Cultivated in culturing liquid in bioreactor.
However, present inventor has found, and due to being influenceed by the density of photo-biological in culturing liquid, light penetration cultivation
Decay rapidly during liquid, the distance that light can be penetrated is only several millimeters so that on vertical smooth surface direction, only near smooth surface
Several millimeters of region is light area, outside be all dark space, can so cause part photo-biological in bioreactor for a long time at
Yu Guangqu or dark space, and the photosynthesis of photo-biological include two stages of light reaction and dark reaction, photo-biological circulate into
Row light reaction and dark reaction can complete photosynthesis, and photo-biological cannot be circulated carries out light reaction and dark reaction, is unfavorable for light
Biology carries out photosynthesis, is unfavorable for improving the cultured output of photo-biological.
The content of the invention
It is an object of the invention to provide a kind of bioreactor, the cultured output for improving photo-biological.
To reach above-mentioned purpose, the invention provides a kind of bioreactor, adopt the following technical scheme that：
A kind of bioreactor, including hollow reactor body, the reactor body have at least one light
Face, the bioreactor also includes the aerator in the reactor body, and the aerator is used for court
To the direction jet of smooth surface.
Include aerator in the bioreactor that the present invention is provided, the aerator is used for towards the side of smooth surface
To jet, so that culturing liquid can take exercises along the jet direction of aerator, make culturing liquid between dark space and light area
Flowing is circulated, and then drives the photo-biological in culturing liquid to be shifted between dark space and light area, i.e., adjusted by jet
, in light area and the cycle frequency of dark space, control photo-biological is in light area and dark space residence time so that in culturing liquid for section photo-biological
Photo-biological will not for a long time be in light area or dark space, and then enable that photo-biological is circulated and carry out light reaction and dark reaction, have
Photosynthesis is carried out beneficial to photo-biological, is conducive to improving the cultured output of photo-biological.
Brief description of the drawings
In order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, embodiment will be described below
Needed for the accompanying drawing to be used be briefly described, it should be apparent that, drawings in the following description are only more of the invention
Embodiment, for those of ordinary skill in the art, on the premise of not paying creative work, can also be attached according to these
Figure obtains other accompanying drawings.
Fig. 1 is the schematic diagram of the first bioreactor provided in an embodiment of the present invention；
Fig. 2 is the schematic diagram of feeder provided in an embodiment of the present invention；
Fig. 3 is the schematic diagram of aeration tube provided in an embodiment of the present invention；
Fig. 4 be attachment structure provided in an embodiment of the present invention and its with aeration tube and the annexation schematic diagram of gas outlet；
Fig. 5 is the schematic diagram of second bioreactor provided in an embodiment of the present invention；
Fig. 6 is the schematic diagram of the third bioreactor provided in an embodiment of the present invention.
Description of reference numerals：
1-reactor body；11-smooth surface；2-aerator；
21-feeder；211-air inlet；212-gas outlet；
22-aeration tube；221-pipe；222-end face；
223-the first passage；224-loop configuration；225-the second passage；
23-attachment structure.
Specific embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation is described, it is clear that described embodiment is a part of embodiment of the invention, rather than whole embodiments.Based on this hair
Embodiment in bright, the every other implementation that those of ordinary skill in the art are obtained under the premise of creative work is not made
Example, belongs to the scope of protection of the invention.
Embodiment one
A kind of bioreactor is the embodiment of the invention provides, as shown in figure 1, the bioreactor includes：It is hollow
Reactor body 1, reactor body 1 have at least one smooth surface 11, the bioreactor also include be located at reactor
Aerator 2 in body 1, the aerator 2 is used for the direction jet towards smooth surface 11.Wherein, aerator 2 sprays
Gas be preferably the mixed gas of air and carbon dioxide, in order to avoid the cultivation to photo-biological has a negative impact.The photo-biological
Reactor can be used for cultivating microalgae, such as Nannochloropsis oculata.
Include aerator in bioreactor provided in an embodiment of the present invention, the aerator is used for towards light
The direction jet in face, so that culturing liquid can take exercises along the jet direction of aerator, makes culturing liquid in dark space and light
Flowing is circulated between area, and then drives the photo-biological in culturing liquid to be shifted between dark space and light area, i.e., by spray
Gas adjusts photo-biological in light area and the cycle frequency of dark space, and control photo-biological is in light area and dark space residence time so that cultivation
Photo-biological in liquid will not for a long time be in light area or dark space, and then enable that photo-biological is circulated and carry out light reaction and dark anti-
Should, be conducive to photo-biological to carry out photosynthesis, be conducive to improving the cultured output of photo-biological.
The concrete structure of above-mentioned aerator 2 can have various, as long as it can be used to be sprayed to towards the direction of smooth surface 11
Gas.Exemplarily, as shown in figure 1, aerator 2 includes hollow feeder 21 and multiple aeration tubes 22, wherein, such as
Shown in Fig. 2, the feeder 21 is all connected with the one-to-one multiple gas outlet 212 of multiple aeration tubes 22, each aeration tube 22
On a gas outlet 212.The feeder 21 can also have at least one air inlet 211, exemplarily, the air admission hole
211 can be connected with air compressor.In photo-biological breeding process, the process of gas flowing is as follows：Carbon dioxide and air
Mixed gas feeder 21 is passed through by the air inlet 211 on feeder 21, then from the gas outlet 212 of feeder 21
Into aeration tube 22, finally sprayed from aeration tube 22, to cause aerator 2 to the direction jet towards smooth surface 11.
Further, since in the breeding process of photo-biological, when the intensity of the light of external light source irradiation changes, light
The distance of the culturing liquid that line can be penetrated can also change, and then cause the light area of culturing liquid and thickness (the Ji Guang areas of dark space
With dark space along the depth perpendicular to smooth surface direction) change.And photo-biological carry out light reaction have photon numbers it is how many will
Ask, usual photo-biological carries out a light reaction needs 8 photons.When intensity of illumination is big, photo-biological obtains light in the unit interval
The quantity of son is more, so photo-biological needs residence time short in light area；When intensity of illumination is weak, photo-biological is within the unit interval
The quantity of the photon of acquisition is few, so photo-biological needs residence time long in light area, light is carried out to absorb enough number of photons
Reaction.If the situation of the gas injection of aerator 2 can not change with intensity of illumination, can not adjust, control photo-biological
In the cycle frequency and residence time of light area and dark space, it is unfavorable for that photo-biological circulation carries out light reaction and dark reaction, is unfavorable for carrying
The cultured output of photo-biological high.
Therefore, in order to solve the above problems, the cultured output of photo-biological is improved, aeration tube 22 is provided in the embodiment of the present invention
Following three kinds of structures, wherein, when aeration tube 22 be the first structure when, the jet amount of aeration tube 22 is adjustable, when aeration tube 22
During for second structure, aeration tube 22 is scalable, so that aeration tube 22 is adjustable with the distance between smooth surface 11, works as aeration
When pipe 22 is the third structure, the distance between aeration tube 22 and smooth surface 11 and jet amount are adjustable.
The first structure, as shown in figure 3, each aeration tube 22 is to include N grades of pipe 221 being set with successively from inside to outside,
The two-stage pipe 221 of arbitrary neighborhood can be relatively rotated circumferentially in direction, and N is the positive integer more than or equal to 2.Specifically, the 1st grade of pipe
One end of son 221 is opening, and the other end is end face 222, and multiple first passages 223 are provided with end face 222；I-stage pipe
221 one end is opening, and the other end is end face 222, has multiple first passages 223 on end face 222 and is managed with the i-th -1 grade
The corresponding circular open of son, and the circular open on the end face 222 of i-stage pipe 221 diameter and the i-th -1 grade pipe 221
Diameter it is identical；And, the i-th -1 grade pipe 221 also includes the loop configuration 224 stretched out from one end of opening, loop configuration
224 external diameter is identical with the diameter of i-stage pipe 221, and multiple second passages 225 are provided with loop configuration 224, and second leads to
Stomata 225 is corresponded with the first passage 223 and size is identical, and i is the positive integer more than or equal to 2 and less than or equal to N.
Aeration tube 22 with the first structure in use, when between any two-stage pipe 221 of mutual sheathing
When circumferentially direction relatively rotates, the i-th -1 grade pipe 221 includes in loop configuration 224 the second passage 225 and i-stage
The position relationship between the first passage 223 on the end face 222 of pipe 221 will change, and the two can be completely superposed,
Now the jet amount of the end face 222 of i-stage pipe 221 is maximum, and the two can partially overlap, now the i-th -1 grade spray of pipe 221
Tolerance is placed in the middle, and the two can also complete misaligned, now the i-th -1 grade pipe 221 not jet.Therefore, when bioreactor
When aeration tube 22 has the first structure, the jet amount of aeration tube 22 is adjustable such that it is able to adjust stop of the photo-biological in light area
The length and photo-biological of time in light area and the cycle frequency of dark space, to adapt to the change of light intensity, to be conducive to photoproduction
Thing circulation carries out light reaction and dark reaction, and then improves the cultured output of photo-biological.
Second structure, as shown in figure 3, each aeration tube 22 is to include N grades of pipe 221 being set with successively from inside to outside,
The two-stage pipe 221 of arbitrary neighborhood can be relatively moved in axial direction, and N is the positive integer more than or equal to 2.Specifically, the 1st grade of pipe
One end of son 221 is opening, and the other end is end face 222, and multiple first passages 223 are provided with end face 222；I-stage pipe
221 one end be opening, the other end be end face 222, on end face have multiple first passages 223 and with the i-th -1 grade pipe
The diameter of the circular open on corresponding circular open, and the end face 222 of i-stage pipe 221 and the i-th -1 grade pipe 221
Diameter is identical, and i is the positive integer more than or equal to 2 and less than or equal to N.
With second aeration tube of structure 22 in use, when between any two-stage pipe 221 of mutual sheathing
When in axial direction relatively moving, you can between the end face 222 and smooth surface 11 of the pipes at different levels 221 in aeration tube 22 away from
From being adjusted, the compression and stretching of aeration tube 22 are realized, and then length of the photo-biological in the residence time in light area can be adjusted
And photo-biological is in light area and the cycle frequency of dark space, to adapt to the change of light intensity, carried out with being conducive to photo-biological to circulate
Light reaction and dark reaction, and then improve the cultured output of photo-biological.
Exemplarily, when light intensity gradually strengthens, should stretch above-mentioned aeration tube 22, the end face of each pipe 221
The distance between 222 and smooth surface 11 reduce, and to cause residence time of the photo-biological in light area shorter, photo-biological is in light area and secretly
The cycle frequency in area is larger, when light intensity gradually weakens, should shrink above-mentioned aeration tube 22, the end face of each pipe 221
The increase of the distance between 222 and smooth surface 11, to cause residence time of the photo-biological in light area more long, photo-biological is in light area and secretly
The cycle frequency in area is smaller, with realize varying strength light irradiate under, photo-biological can circulate carry out light reaction and secretly instead
Should, and then improve the cultured output of photo-biological.
The third structure, as shown in figure 3, on the basis of second structure, the two-stage pipe 221 of arbitrary neighborhood can also edge
Circumferential direction is relatively rotated；And, the i-th -1 grade pipe 221 also includes the loop configuration 224 stretched out from one end of opening, ring
The external diameter of shape structure 224 is identical with the diameter of i-stage pipe 221, and multiple second passages 225 are provided with loop configuration 224,
Second passage 225 is corresponded with the first passage 223 and size is identical, and i is more than or equal to 2 and just whole less than or equal to N
Number.
Aeration tube 22 with the third structure in use, the distance between aeration tube 22 and smooth surface 11 and
Jet amount is adjustable such that it is able to adjust photo-biological in the length and photo-biological of the residence time in light area in light area and dark space
Cycle frequency, to adapt to the change of light intensity, carries out light reaction and dark reaction, and then improve light to be conducive to photo-biological to circulate
Biological cultured output.Its specific regulative mode is described in detail in the first structure and second structure, herein
The description for no longer being repeated.
The maximum gauge of the N grades of pipe 221 referred in above-mentioned three kinds of structures is not more than 10 centimetres, is with 3~5 centimetres
Preferably, and every grade of length of pipe 221 is advisable with 3~5 centimetres.In actual application, with the increasing of bioreactor light path
Plus, the maximum gauge of pipes at different levels 221 is consequently increased, and the series of the pipe 221 that each aeration tube 22 includes is consequently increased,
But should ensure that aeration tube 22 after stretching to light path during maximum length no more than bioreactor.
Additionally, inventors herein have recognized that, when aerator 2 is along the direction jet perpendicular to light area and the boundary of dark space
When, flow effect of the culturing liquid between light area and dark space preferably, but when using natural light as external light source when, light with
Angle between smooth surface 11 also can be over time change and change so that the light area and dark space of culturing liquid become
Change, cause aerator 2 always along the direction jet perpendicular to light area and the boundary of dark space, and then culturing liquid cannot be caused in light
Between area and dark space to circulate effect undesirable.In order to solve the above problems, as shown in figure 4, being carried in the embodiment of the present invention
The bioreactor of confession can also include hollow attachment structure 23, and each aeration tube 22 is connected by an attachment structure 23
It is connected on a gas outlet 212, wherein, the axial direction of one end that attachment structure 23 is connected with aeration tube 22 and the normal of smooth surface 11
Angle between direction is adjustable, to cause the jet direction of its aeration tube 22 for connecting adjustable, and then enables aerator 2
Always along the direction jet perpendicular to light area and the boundary of dark space, to cause culturing liquid circulating between light area and dark space
Effect is preferable.
Realized for the ease of those skilled in the art, the present invention provides a kind of concrete structure of attachment structure 23, exemplary
Ground, as shown in figure 4, one end that attachment structure 23 has the tubular structure of fold, tubular structure for surface is connected to gas outlet 212
On, the other end of tubular structure is connected to the bottom of aeration tube 22.The fold of side is stretched on tubular structure, the pleat of its opposite side
When wrinkle is compressed, so that tubular structure bends such that it is able to the axial direction of the one end being connected with aeration tube 22 to tubular structure
And the angle between the normal direction of smooth surface 11 is adjusted.When aeration tube 22 includes N grades of pipe, the bottom of aeration tube 22
Refer to N grades of one end towards feeder 21 of pipe.Exemplarily, the two ends of tubular structure can be distinguished by screw
It is connected with gas outlet 212 and the bottom of aeration tube 22, now, the two ends and gas outlet 212 and the bottom of aeration tube 22 of tubular structure
All should be provided with screw, the two ends of certain tubular structure can also by bond etc. other modes respectively with gas outlet 212 and expose
The bottom of tracheae 22 connects, and the embodiment of the present invention is not defined to this.
It should be noted that the concrete structure of attachment structure 23 is not limited to this, those skilled in the art can be according to reality
Border needs selection.For example, attachment structure 23 can select the structure described in patent of the Authorization Notice No. for CN201696738U,
Because the structure has been carried out describing in detail in that patent, the embodiment of the present invention is no longer repeated.
Additionally, the need for being cultivated according to photo-biological in practice, bioreactor provided in an embodiment of the present invention can be
Box like structure or tubular structure.Exemplarily, as shown in figure 1, it is box like structure to work as bioreactor, and the photo-biological is anti-
When answering utensil and having two relative smooth surfaces 11, feeder 21 is tabular, and feeder 21 is located in bioreactor
Between, two sides of feeder 21 are respectively facing two smooth surfaces 11, and multiple is respectively provided with two sides of feeder 21
Gas outlet (not shown), is all connected with an aeration tube 22 on each gas outlet.As shown in figure 5, when bioreactor is
Box like structure, and the bioreactor have a smooth surface 11 when, feeder 21 be tabular, feeder 21 be located at light
The side relative with smooth surface 11 in bioreactor, feeder 21 towards having multiple outlets on the side of smooth surface 11
Mouth (not shown), is all connected with an aeration tube 22 on each gas outlet.As shown in fig. 6, when bioreactor is tubular
Structure, and tubular structure side be smooth surface 11 when, feeder 21 be rectangular structure or cylindrical structure, supply dress
Put 21 to be located on the axis of bioreactor, be respectively provided with multiple gas outlets on each side of feeder 21 and (do not show in figure
Go out), an aeration tube 22 is all connected with each gas outlet.
Embodiment two
For the ease of skilled artisan understands that the advantage of the bioreactor in the present invention, in the embodiment of the present invention
To carrying out the cultured output of Nannochloropsis oculata cultivation using the bioreactor in the present invention, and use prior art
In bioreactor carry out the cultured output of Nannochloropsis oculata cultivation and contrasted.
Contrast experiment one
The bioreactor as shown in Figure 1 provided using the present invention carries out Nannochloropsis oculata cultivation, the photoproduction
Thing reactor is box like structure, and the bioreactor has two relative smooth surfaces 11, and feeder 21 is tabular, is supplied
Device of air 21 is located at the centre of bioreactor, and two sides of feeder 21 are respectively facing two smooth surfaces 11, supplies
Multiple gas outlets 212 are respectively provided with two sides of device 21, an aeration tube 22 is all connected with each gas outlet 212.The light
The specification of bioreactor is 1 meter of (length) × 1 meter (height) × 0.2 meter (width), and the specification of feeder 21 is 1 meter of (length) × 0.8
Rice (height) × 0.02 meter (width).Using natural light as external light source, two smooth surfaces 11 of bioreactor court respectively
It is that 1 g/l of algae solution is cultivated to initial inoculation concentration using the bioreactor, and utilize air to east-west direction
Compressor is to the mixed gas that carbon dioxide and oxygen is passed through in feeder 21.
Breeding process is specific as follows：First, 8 points or so of the morning, the aeration tube 22 towards east is stretched, and stretches aeration tube 22
Its first order pipe distance towards the centimeters of smooth surface 1~3 in east is grown to, then rotates pipes at different levels 221, by pipes at different levels 221
Including loop configuration 224 on end face 222 of second passage 225 with next stage pipe 221 on the first passage 223
Registration is adjusted to 1/4；Secondly, at eight between ten one points, per every other hour, by towards the axial direction of each aeration tube 22 in east
And the angle between the normal direction of smooth surface 11 is increased up 15 degree, while by the first passage 223 and the second passage 225
Registration increase by 1/4；Again, since ten four points or so of afternoon, will be shunk towards the aeration tube 22 in east, and shorten aeration tube 22
To its first order pipe distance towards the centimeters of smooth surface 5~7 in east, while the aeration tube 22 being exposed to the west is stretched, make aeration tube 22
It is elongated to the centimeters of smooth surface 1~3 that its first order pipe distance is exposed to the west, then by the axial direction of each aeration tube 22 and smooth surface 11
Normal direction between angle be adjusted up being 45 degree, while make the first passage 223 and the second passage 225 completely weigh
Close；Finally, between ten four points to ten seven points, per every other hour, by the axial direction of each aeration tube 22 and the normal of smooth surface 11
Angle between direction reduces downwards 15 degree, while the registration of the first passage 223 and the second passage 225 is reduced into 1/4.
When carrying out Nannochloropsis oculata cultivation using the bioreactor of prior art, the area with above-mentioned breeding process
It is not only that in bioreactor without aerator.
The bioreactor as shown in Figure 1 provided using the present invention cultivates Nannochloropsis oculata continuous five days, eye
The yield per unit area that point intends Nannochloropsis oculata reaches 25.6 grams/m/day, and phototranstormation efficiency is 4.8%.And use existing skill
Continuous five days of the bioreactor cultivation Nannochloropsis oculata of art.The yield per unit area of Nannochloropsis oculata is 15.8
Gram/m/day, phototranstormation efficiency is 3.8%.By data above as can be seen that the optical-biological reaction provided using the present invention
Device is cultivated to Nannochloropsis oculata, substantially increases the cultured output of Nannochloropsis oculata.
Contrast experiment two
The bioreactor as shown in Figure 5 provided using the present invention carries out Nannochloropsis oculata cultivation, the photoproduction
Thing reactor is box like structure, and the bioreactor has a smooth surface 11, and feeder 21 is tabular, feeder
21 are located at sides relative with smooth surface 11 in bioreactor, feeder 21 towards having on the side of smooth surface 11
Multiple gas outlets 212, are all connected with an aeration tube 22 on each gas outlet 212.The specification of the bioreactor is 1 meter (length)
× 1 meter of (height) × 0.1 meter (width), the specification of feeder 21 is 1 meter of (length) × 0.8 meter (height) × 0.02 meter (width).Using certainly
Right light is used as external light source, and the smooth surface 11 of the bioreactor is dense to initial inoculation using the bioreactor towards south
The algae solution spent for 1 g/l is cultivated, and using air compressor to being passed through the mixed of carbon dioxide and oxygen in feeder 21
Close gas.
Breeding process is specific as follows：First, 8 points or so of the morning, aeration tube 22 is stretched, and aeration tube 22 is elongated to it
First order pipe is apart from the centimeters of smooth surface 1~3, then rotates pipes at different levels 221, the ring junction that pipes at different levels 221 are included
The second passage 225 on structure 224 is adjusted to 1/ with the first overlapping for passage 223 on the end face 222 of next stage pipe 221
4, and each aeration tube 22 adjusts eastwards, so that the angle between the normal direction of its axial direction and smooth surface 11 is 60 degree；Secondly,
At eight between ten one points, per every other hour, each aeration tube 22 is axially westwards adjusted 15 degree, while first is ventilated
The registration of the passage 225 of hole 223 and second increases by 1/4；Again, since ten four points or so of afternoon, by each aeration tube 22
Axially westwards adjust 15 degree, aeration tube 22 is shunk, aeration tube 22 is shortened to its first order pipe apart from smooth surface 3~5
Centimeters；Finally, between ten four points to ten seven points, per every other hour, aeration tube 22 is axially westwards adjusted 15 degree, while
The registration of the first passage 223 and the second passage 225 is reduced 1/4.
When carrying out Nannochloropsis oculata cultivation using the bioreactor of prior art, the area with above-mentioned breeding process
It is not only that in bioreactor without aerator.
The bioreactor as shown in Figure 4 provided using the present invention cultivates Nannochloropsis oculata continuous five days, eye
The yield per unit area that point intends Nannochloropsis oculata reaches 28.8 grams/m/day, and phototranstormation efficiency is 5.4%.And use existing skill
Continuous five days of the bioreactor cultivation Nannochloropsis oculata of art.The yield per unit area of Nannochloropsis oculata is 16.3
Gram/m/day, phototranstormation efficiency is 4.0%.By data above as can be seen that the optical-biological reaction provided using the present invention
Device is cultivated to Nannochloropsis oculata, substantially increases the cultured output of Nannochloropsis oculata.
From two above contrast experiment, the bioreactor provided using the present invention can greatly improve photo-biological
Cultured output.
The above, specific embodiment only of the invention, but protection scope of the present invention is not limited thereto, and it is any
Those familiar with the art the invention discloses technical scope in, change or replacement can be readily occurred in, should all contain
Cover within protection scope of the present invention.Therefore, protection scope of the present invention should be based on the protection scope of the described claims.
Claims (10)
1. a kind of bioreactor, including hollow reactor body, the reactor body has at least one smooth surface,
Characterized in that, the bioreactor also includes the aerator in the reactor body, the aerator
For to the direction jet towards the smooth surface, the aerator to include hollow feeder and multiple aeration tubes, institute
It is to include N grades of pipe being set with successively from inside to outside to state aeration tube, and N is the positive integer more than or equal to 2, the two-stage pipe of arbitrary neighborhood
Son can be relatively moved in axial direction and/or circumferential direction is relatively rotated；
Wherein, the 1st grade of one end of pipe is opening, and the other end is end face；One end of i-stage pipe is opening, and the other end is end
Face, multiple first passages are provided with per the end face of one-level pipe, and the i-th -1 grade pipe also includes stretching out from one end of opening
Loop configuration, the loop configuration is provided with multiple second passages, and i is the positive integer more than or equal to 2 and less than or equal to N.
2. bioreactor according to claim 1, it is characterised in that the feeder includes and multiple aeration tubes
The multiple gas outlets for connecting one to one.
3. bioreactor according to claim 2, it is characterised in that the two-stage pipe of arbitrary neighborhood can side vertically
To relative movement；
Wherein, i-stage pipe has the circle on the circular open corresponding with the i-th -1 grade pipe, and the end face of i-stage pipe
The diameter of opening is identical with the diameter of the i-th -1 grade pipe.
4. bioreactor according to claim 3, it is characterised in that the two-stage pipe of arbitrary neighborhood can circumferentially side
To relative rotation；And, the i-th -1 grade external diameter of the loop configuration of pipe is identical with the diameter of i-stage pipe, second passage
Corresponded with first passage and size is identical.
5. bioreactor according to claim 2, it is characterised in that the two-stage pipe of arbitrary neighborhood can circumferentially side
To relative rotation；
Wherein, i-stage pipe has the circle on the circular open corresponding with the i-th -1 grade pipe, and the end face of i-stage pipe
The diameter of opening is identical with the diameter of the i-th -1 grade pipe；
And, the i-th -1 grade external diameter of the loop configuration of pipe is identical with the diameter of i-stage pipe, is provided with the loop configuration
Multiple second passages, second passage is corresponded with first passage and size is identical.
6. bioreactor according to claim 2, it is characterised in that also including hollow attachment structure, each institute
State aeration tube to be connected on a gas outlet by an attachment structure, the attachment structure and the aeration tube
Angle between the normal direction of the axial and described smooth surface of one end of connection is adjustable.
7. bioreactor according to claim 6, it is characterised in that the attachment structure has fold for surface
Tubular structure, one end of the tubular structure is connected on the gas outlet, and the other end of the tubular structure is connected to described
Aeration bottom of the tube.
8. the bioreactor according to claim any one of 2-7, it is characterised in that the bioreactor is case
Shape structure, the bioreactor has two relative smooth surfaces, and the feeder is tabular, the feeder position
In the centre of the bioreactor, two sides of the feeder are respectively facing two smooth surfaces, the confession
Multiple gas outlets are respectively provided with two sides of device of air, an aeration tube is all connected with each described gas outlet.
9. the bioreactor according to claim any one of 2-7, it is characterised in that the bioreactor is case
Shape structure, the bioreactor has a smooth surface, and the feeder is tabular, and the feeder is located at described
The side relative with the smooth surface in bioreactor, has on the side of the direction smooth surface of the feeder
Multiple gas outlets, are all connected with an aeration tube on each described gas outlet.
10. the bioreactor according to claim any one of 2-7, it is characterised in that the bioreactor is
Tubular structure, the side of the tubular structure is smooth surface, and the feeder is rectangular structure or cylindrical structure, institute
Feeder is stated on the axis of the bioreactor, is respectively provided with described in multiple on each side of the feeder
Gas outlet, is all connected with an aeration tube on each described gas outlet.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510357644.0A CN104928152B (en) | 2015-06-24 | 2015-06-24 | A kind of bioreactor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510357644.0A CN104928152B (en) | 2015-06-24 | 2015-06-24 | A kind of bioreactor |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104928152A CN104928152A (en) | 2015-09-23 |
| CN104928152B true CN104928152B (en) | 2017-06-09 |
Family
ID=54115581
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510357644.0A Active CN104928152B (en) | 2015-06-24 | 2015-06-24 | A kind of bioreactor |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104928152B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU218510U1 (en) * | 2023-04-05 | 2023-05-29 | Борис Павлович Глушак | Photobioreactor |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2012191894A (en) * | 2011-03-17 | 2012-10-11 | Ihi Corp | Culture apparatus |
| CN103525686A (en) * | 2013-10-24 | 2014-01-22 | 重庆大学 | Composite reinforced microalgae photobioreactor based on hollow light pipes |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20090047722A1 (en) * | 2005-12-09 | 2009-02-19 | Bionavitas, Inc. | Systems, devices, and methods for biomass production |
-
2015
- 2015-06-24 CN CN201510357644.0A patent/CN104928152B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2012191894A (en) * | 2011-03-17 | 2012-10-11 | Ihi Corp | Culture apparatus |
| CN103525686A (en) * | 2013-10-24 | 2014-01-22 | 重庆大学 | Composite reinforced microalgae photobioreactor based on hollow light pipes |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU218510U1 (en) * | 2023-04-05 | 2023-05-29 | Борис Павлович Глушак | Photobioreactor |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104928152A (en) | 2015-09-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN201245657Y (en) | Photobioreactor for microalgae cultivation | |
| CN101402915B (en) | High-efficiency photobioreactor for cultivation of photosynthetic bacteria | |
| US20120021505A1 (en) | Circulatory photobioreactor | |
| CN106318853A (en) | Self-cleaned spiral airlift inner-loop photobioreactor | |
| US20100144019A1 (en) | Photobioreactor | |
| KR100818203B1 (en) | Split-column circulating photobioreactor and method for culturing photosynthetic microorganisms using the same | |
| KR100283026B1 (en) | Photobioreactor | |
| JP5657938B2 (en) | Circulating culture method for photosynthetic microalgae | |
| CN103773673A (en) | Cylindrical air-lift type high-efficient photobioreactor for microalgae culture and application thereof | |
| CN104152344A (en) | Photobioreactor used for culturing benthic microalgae and culturing method thereof | |
| CN202415535U (en) | Scale microalgae cultivation bioreactor | |
| CN104928152B (en) | A kind of bioreactor | |
| CN206665285U (en) | A kind of supporting pipeline formula bioreactor operates support | |
| KR20160031839A (en) | Photoreactive Apparatus and method for culturing microalgae | |
| JP5324532B2 (en) | Circulating photobioreactor | |
| CN208844056U (en) | A kind of tubular type bioreactor for cultivating microalgae | |
| CN104789631A (en) | Chlorella culture method capable of increasing lutein content and apparatus | |
| CN102660449A (en) | Sleeve-type photo-bioreactor | |
| CN102517212A (en) | Guide flow cylinder, cylindrical bioreactor using guide flow cylinder and method for arranging guide flow cylinder | |
| CN103131627A (en) | Light organism reaction device and application | |
| CN102344888B (en) | Circulating type photobioreactor | |
| CN209481643U (en) | One seeds algae bucket | |
| CN102864070A (en) | Adjustable photobioreactor for high-density culture of microalgae | |
| CN202865219U (en) | Adjustable micro-alga high-density culture photobioreactor | |
| CN206706102U (en) | A kind of novel photo-biological reactor |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |