CN102344888B - Circulating type photobioreactor - Google Patents

Circulating type photobioreactor Download PDF

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Publication number
CN102344888B
CN102344888B CN201010248859.6A CN201010248859A CN102344888B CN 102344888 B CN102344888 B CN 102344888B CN 201010248859 A CN201010248859 A CN 201010248859A CN 102344888 B CN102344888 B CN 102344888B
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China
Prior art keywords
culture
cultivation
import
bioreactor
culture solution
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CN102344888A (en
Inventor
金光浩
姜憙奎
权宁一
金善钟
林希贞
金美廷
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Kailuosi Global Co.,Ltd.
Industry Academic Cooperation Foundation of Eulji University
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KAILUOSI GLOBAL CO Ltd
Industry Academic Cooperation Foundation of Eulji University
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02Photobioreactors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/18External loop; Means for reintroduction of fermented biomass or liquid percolate
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M31/00Means for providing, directing, scattering or concentrating light
    • C12M31/02Means for providing, directing, scattering or concentrating light located outside the reactor

Abstract

The present invention provides a circulating type photobioreactor. The circulating type photobioreactor comprises a first culture part, a second culture part and a pump part used for connecting the first culture part and the second culture part. The first culture part comprises a culture pot used for supplying a medium and a first light source which irradiates the inner culture pot and is coupled with the culture pot. The second culture part comprises a culture tube arranged outside the culture pot used for supplying the culture from the culture pot and a second light source which irradiates the inner culture tube and is coupled with the culture tube. The pump part is connected with the first culture part and the second culture part for circulating a culture solution between the two parts.

Description

Circulating bioreactor
Background of invention
1. invention field
Following specification sheets relates to culture apparatus, particularly circulating bioreactor.
2. the description of association area
At present, the crop of planting in world wide is used as the mankind's Major Foods source.But the output in every acre of soil is not too high, the energy efficiency of sun power is extremely low.On the contrary, it is favourable in water, can utilizing the micro-algae of photosynthesis of sun power, carbonic acid gas and a small amount of inorganic salt growth, because cultivate in the place that they can not be grown crop, the protein yield of every acre is made at least 20 times of object heights than routine, and various useful matteies and the rare crude substance that can not produce in microorganism, animal and plant can be provided.Specifically, because alga cells size is larger, can precipitate well, therefore separated they and therefrom to extract some materials be easy.In addition, they as main energy sources, therefore may utilize sun power with sun power efficiently.In addition,, because they use carbonic acid gas as carbon source, there is the photosynthesizer producing oxygen as by product, so they can alleviate air pollution.
Therefore, the micro-algae of photosynthesis that comprises Chlorella (Chlorella), Dunaliella (Dunaliella) and Spirullina (Spirulina) particularly micro-algae of Spirullina (hereinafter referred to as " spirulina ") to have become research popular, because they are larger than the micro-algae stature of other photosynthesis, easily in alkaline pollution environment, grow, can be applicable to food, medicine and other industry.Meanwhile, there is such as making a clear distinction between the four seasons, between season temperature and change the Temperate Region in China (as Korea S) of climate characteristic greatly sunshine, developing indoor cultivation technology rather than outdoor culture technique.
For example, Korean Patent discloses bactogen No. 235182, and this device comprises auto controlled heater, have the culture vessel of breather, have shelf for supporting the equipment of culture vessel, be coupled to the substratum supply pipe of the side of shelf, for the rubber hose of Cyclic culture solution with for the water pump of Cyclic culture solution.Korean Patent communique discloses for No. 2004-0019298 for cultivating the device of micro-algae, this device comprises the culture vessel that is made into bicylindrical type, it is comprised of interior cylinder (horizontal positioned) and outside cylinder, wherein at least outside cylinder comprises the transparent material of transmissive light, and gas feed is opened on the bottom of culture vessel.Korean Patent discloses the outdoor culture vessel of type water course for micro-algae for No. 679989, and wherein integrative installation technology is useful on the inoculation culture container of cultivating inoculum.But if cultivate spirulina with these devices, the spirulina of growth can excessively be attached at the surface of culture vessel.Therefore, culture efficiency step-down, and due to various microbiological contamination, can not obtain favourable cultured products.
In order to address the above problem, developed such method, it comprises culture vessel sealing, and prevents various microbiological contamination by filter the air of supply with strainer.For example, Korean Patent communique discloses outdoor large scale culturing device No. 2002-0057882, and this device comprises for filtering equipment, pneumatic pump, filter plant, conduit, strainer, needle-valve and the culture vessel with ultraviolet sterilization; Korean Patent communique discloses high-density culture device No. 2002-0083558, and this device is included in culture vessel, the pH sensor in this culture vessel and the divider on its top with lid, the shelf with luminescent lamp, pH controller, pneumatic pump and the CO2 culture tank of this culture vessel.
Summary of the invention
Although the various devices under application prior art may prevent various microbiological contamination, but being excessively attached at culture vessel internal surface, the micro-algae of photosynthesis cause the problem of culture efficiency decline to be not yet resolved so far.If problem is resolved, may obviously improve the culture efficiency of the micro-algae of photosynthesis.But, do not instruct the report of this solution.Therefore, thus the object of this invention is to provide and can prevent that the micro-algae of photosynthesis is attached at micro-algae that culture vessel internal surface improves culture efficiency and cultivates and use novel culture apparatus.
The present invention attempts to address the above problem and complete the present invention, and work as the micro-algae of photosynthesis while cultivating in a looping fashion by confirmation, the area that culture solution is exposed to light is maximized and the micro-algae of photosynthesis that grows can not be attached at culture vessel internal surface and confirm the present invention.
In one aspect, the invention provides circulating photosynthesis bio-reactor, this bio-reactor comprises:
First cultivates part, and this first cultivation part comprises the culture tank that wherein substratum is supplied, and is coupled to culture tank to irradiate the first light source of culture tank inside;
Second cultivates part, and this second is cultivated part and comprise that to be arranged on culture tank outside and by the culture tube from culture tank supply culture solution, and is coupled to culture tube to irradiate the secondary light source of culture tube inside; With
Pump part, this pump part is cultivated part with the first cultivation part and second and is connected, so that culture solution is cultivated between part and circulated in the first cultivation part and second.
In one embodiment, the first light source is arranged on culture tank inside, but is not limited to such setting.In one embodiment, secondary light source is coupled to the internal surface of culture tube or is arranged on culture tube outside along culture tube longitudinal direction, but is not limited to such setting.In another embodiment, culture tube preferably has parallel repeatedly folded form, comprises a plurality of secondary light sources that are coupled to culture tube outside along culture tube longitudinal direction, but is not limited to such setting.In a preferred embodiment, the internal diameter of culture tube is 3-30cm.In a preferred embodiment, internal diameter is 5-20cm.In the most preferred embodiment, internal diameter is 10-15cm.In a preferred embodiment, first cultivates at least one first culture solution import and at least one the first culture solution outlet that part also comprises that both are connected with culture tank and pump part, second cultivates part also comprises respectively and exporting with at least one the second culture solution import being connected with the second culture solution import and at least one second culture solution by pump part and the outlet of the first culture solution, but is not limited to such setting.In another preferred embodiment, first cultivates part also comprises for fresh culture being fed to fresh culture import and the final outlet for culture solution is discharged from culture tank of culture tank, but be not limited to such setting, and fresh culture import preferably has the shape of spraying ball, this spraying bag is drawn together bulb and the many tiny nozzles on this bulb, but is not limited to such setting.
In one embodiment, first cultivates part also comprises the agitator for mixed culture solution that is connected to culture tank inside, but is not limited to such setting.In another embodiment, first cultivates part also comprises that this pressure controlled valve is preferably check valve for controlling the pressure controlled valve of the pressure of culture tank, by the oxygen pressure producing in the micro-algae culturing process of photosynthesis, opens.
In one embodiment, first cultivates part also comprises that this temperature regulator is preferably the water jacket that is coupled to culture tank outside for controlling the temperature regulator of the temperature of culture tank, but is not limited to such setting.
In one embodiment, first cultivates part also comprises that one or more sensors that supply are coupled to the port of culture tank, but is not limited to such setting.In another embodiment, first cultivates part also comprises and is coupled to culture tank with by the gas feed in gas inject culture tank, but is not limited to such setting.
In one embodiment, second cultivates part also comprises oxygen escaper, this oxygen escaper comprises grounding sensor (earth sensor), the mass flow controller and the valve being connected with mass flow controller that are electrically connected to grounding sensor, but is not limited to such setting.
In a preferred embodiment, flow speed controller is coupled to pump part, the flow velocity with controlled circulation to the culture solution of the second cultivation part.In a preferred embodiment, flow speed controller is built in pump part.
In yet another aspect, the invention provides circulating photosynthesis bio-reactor, this bio-reactor comprises:
First cultivates part, and this first cultivation part comprises culture tank, the first substratum import, the first substratum outlet and the first light source that wherein substratum is supplied, and described import, outlet and light source are coupled to culture tank separately;
Second cultivates part, this second is cultivated part and comprises that with tubular form, to be arranged in culture tank outside and by the culture tube from culture tank supplied with medium, the second substratum import, the second substratum outlet and secondary light source, described import, outlet and light source are coupled to culture tube separately, wherein the first substratum outlet is connected with the second substratum import, the first substratum import is connected with the second substratum outlet, makes substratum to cultivate between part and to circulate in the first cultivation part and second.
Photosynthesis bio-reactor also can comprise that connecting first cultivates that part and second is cultivated part so that culture solution cultivates in the first cultivation part and second the pump part circulating between part.
In a preferred embodiment, pump part also comprises the flow speed controller of the pump that is electrically coupled to pump part.In a preferred embodiment, flow speed controller is built in pump part.In a preferred embodiment, the internal diameter of culture tube is 3-30cm.In a preferred embodiment, internal diameter is 5-20cm.In the most preferred embodiment, internal diameter is 10-15cm.
Circulating bioreactor of the present invention can prevent that the micro-algae of photosynthesis is attached at the internal surface of culture vessel, thereby can improve the culture efficiency of the micro-algae of photosynthesis.Therefore, can cultivate economically the micro-algae of photosynthesis.
In this manual, " substratum " means not the substratum for cultivating containing the biology (as micro-algae) of any work, and " culture solution " means the mixture of substratum and micro-algae to be cultivated.
Accompanying drawing summary
Accompanying drawing combines with this specification sheets exemplary of the present invention is described, and combines to set forth principle of the present invention with " detailed Description Of The Invention ".
Fig. 1 is the schematic diagram that shows bioreactor according to an embodiment of the invention.
Fig. 2 is the first cultivation sectional view partly that bioreactor of the present invention comprises.
Fig. 3 be explanation tubular type bioreactor comprise second cultivate the orthographic plan of an embodiment of part.
Fig. 4 is the schematic diagram that an embodiment of the oxygen escaper that is coupled to culture tube is described.
Fig. 5 is the orthographic plan that an embodiment of the cultivation part with two the first culture solution outlets is described.
Detailed Description Of The Invention
In following detailed Description Of The Invention, only in illustrational mode, show and describe some exemplary embodiment of the present invention.Those of skill in the art will recognize that the present invention can embody in many different forms, and should not be construed as limited to the embodiment providing herein.Also have, in the middle of the application's book, when certain parts be called as another parts " on " time, it can be directly on another parts, or can between be connected on another parts, have one or more parts between two parties between between them.In the middle of this specification sheets, same reference number represents same parts.
The inventor has carried out various experiments, to study the micro-algae of photosynthesis of cultivation, is attached at the reason on culture vessel internal surface.Results verification two reasons.First reason is thought according to the micro-algae propagation and the growth thereof that apply periodicity optical condition and dark condition.Another reason thinks that the micro-algae of photosynthesis can be coupled to the slow position of velocity of flow, because the mobile local restricted of culture solution, so sticking portion expands
The inventor attempts design can reduce the rate of propagation of the micro-algae of photosynthesis and guarantee culture solution suitable mobile culture vessel by only applying optical condition.But the inventor confirms, for the bioreactor with the culture tank type of agitator, also can not obtain suitably flowing of culture solution even if improve stirring velocity.
Therefore, the inventor attempts with additive method rather than only with agitator, the culture solution that contains the micro-algae of photosynthesis is flowed, confirmation is recycled to from culture tank the pipe that two ends are all coupled to culture tank by culture solution, with the method phase specific energy of only using agitator, culture solution is flowed well.Therefore, the inventor invents and has with the culture tube of light source and the bioreactor of pump, to address the above problem.The inventor can make culture solution flow with constant speed with the culture tube of described bioreactor and pump, and easily culture solution is applied to optical condition by being coupled to the light source of culture tube, provides sufficient light to cultivate the micro-algae of photosynthesis.
Referring to accompanying drawing, describe in detail according to the bioreactor of embodiment of the present invention.
Fig. 1 is the schematic diagram that shows bioreactor according to an embodiment of the invention.As shown in Figure 1, bioreactor can comprise the first cultivation part 100, pump part 200 and the second cultivation part 300.The micro-algae of photosynthesis is by being recycled to pump part 200 from the first cultivation part 100, from pump part 200, be recycled to the second cultivation part, then from the second cultivation, be partly recirculated to the first cultivation part 100 and cultivate, because the first cultivation part 100, pump part 200 and the second cultivation part 300 are interconnected.
Second cultivates part 300 can be spaced apart with the first cultivation part 100.First cultivates part 100 can provide with cylinder shape as shown in Figure 1, but is not limited to such setting.For example, the first shape variable shape of cultivating part 100 is various forms, as Polygons post.Second cultivates part 300 can provide with tubular form, can comprise the pipe made from different shape, etc.Pump part 200 is configured such that culture solution can circulation between the first cultivation part 100 and the second cultivation part 300.For example, pump part 200 can be arranged on the first cultivation part 100 and second and cultivate between part 300, can be equipped with the first culture solution of the first cultivation part 100 and export 132 the 3rd substratum imports 210 that are connected, and can be equipped with the 3rd culture solution outlet 230 being connected with pump 220 and the second second culture solution import 310 of cultivating part 300.Therefore, the culture solution flowing out from the first cultivation part 100 is transferred to the second cultivation part 300, and culture solution circulates as follows: first cultivates part 100 → pump part 200 → the second cultivates part 300 → the first cultivation parts 100.Pump part 200 can be by the flow speed controller controlled circulation with its electrical coupling in the flow velocity of the culture solution of the second cultivation part 300.In a preferred embodiment, flow speed controller is built in pump part 200.The flow velocity of controlling culture solution is important for successfully cultivating spirulina, because spirulina is cellulous spirrillum algae.If flow velocity is low, because hydrokinetics situation is inappropriate, spirulina can occur adherent, gaseous interchange and radiation situation variation.On the contrary, high flow rate can cause the loss of useful matter in spirulina.Therefore, flow velocity should suitably be controlled.Preferably by flow rate control between 1-50cm/s.More preferably, flow velocity is 10-40cm/s.In the most preferred embodiment, flow velocity is 20-30cm/s.
In a preferred embodiment, the internal diameter of culture tube 330 is 3-30cm.In a preferred embodiment, internal diameter is 5-20cm.In the most preferred embodiment, internal diameter is 10-15cm.The internal diameter of culture tube is important for cultivating spirulina.If internal diameter surpasses 30cm, because irradiation situation is inappropriate, productivity variation.On the contrary, if internal diameter is less than 5cm, be difficult to amplification culture volume.
Fig. 2 be bioreactor of the present invention comprise first cultivate the sectional view of part 100.As shown in Figure 2, the first cultivation part 100 can be equipped with cylindrical culture tank 101, inoculum import 110, gas feed 111, sensor port 120, the first culture solution import 131, the first culture solution outlet 132, final outlet 133, pressure controlled valve 112, fresh culture import 134, the first light source 140, agitator 150 and temperature regulator 160.
For example, top, gas feed 111 and the sensor port 120 that inoculum import 110 can be coupled to culture tank 101 can be coupled to the bottom of culture tank 101.But this layout provides as an embodiment, can be changed according to the change of the shape of culture tank 101.
Can provide inoculum import 110, so that various gas is injected to the inside of culture tank 101 as nitrogen and carbon dioxide gas mixture.Therefore,, by keep internal positive pressure in culture tank 101, may in the culturing process of the micro-algae of photosynthesis, prevent from deriving from the various microbiological contamination of outside atmosphere.Malleation can be the approximately 0.1-1.0kg/cm2f for the cellar culture of micro-algae, but is not limited to this scope.
Sensor port 120 can be equipped with various sensors, as pH sensor, CO 2sensor, dissolved oxygen sensor and temperature sensor.
Meanwhile, culture solution partly flows into the first cultivation part 100 by the first culture solution import 131 from the second cultivation, and flows out the first cultivation part 100 to pump part 200 by the first culture solution outlet 132.When cultivating end, final culture solution is discharged into the outside of the first cultivation part 100 by final outlet 133.
In addition, pressure controlled valve 112 is served as reasons and is cultivated the check valve that oxygen pressure that the micro-algae of photosynthesis produces carries out gate (gating), it can be configured to when internal pressure remains malleation by the outside emission gases of gate, but when internal pressure declines, is closed to stop emission gases.
Fresh culture import 134 is excellent type pipes, and its end is spherical, and the shape of the ball of can spraying provides, and has many tiny nozzles on the surface of bulb.Fresh culture can the form with micro-spraying be assigned in the first cultivation part 100 by spraying ball, so first effect of cultivating the foam producing in part 100 of eliminating is played in fresh culture import 134.In addition, fresh culture import 134 can be used for supplying detergent, to wash the inside of the first cultivation part 100, pump part 200 and the second cultivation part 300.
The first light source 140 is for carrying out the device of photosynthetic light for sending in the process of cultivating the micro-algae of photosynthesis, it can send the light of three kinds of wavelength or the light of five kinds of wavelength.For this reason, preferably exposure intensity and light-dark cycle is controlled automatically according to culture condition.For example, the first light source 140 can be arranged on the inside of culture tank 101.In another embodiment, if culture tank 100 comprise that some part of transparent material or culture tank 100 is equipped with can be by the transparent window of light, the first light source 140 can be arranged on the outside of culture tank 101.
Agitator 150 can be coupled to bottom in tank, and effect is mix the culture solution in first cultivation and remaining culture mixture in the first cultivation part 100 is mixed with the culture solution flowing into from the first culture solution import 131.Temperature regulator 160 can be coupled to the outside of the first cultivation part 100, plays the effect of controlling temperature.Temperature regulator can be water jacket, and it can be by making the water cycle of proper temperature control culture temperature by chuck, but be not limited to water jacket.In addition, also can assemble in addition view port, to check that by view port first cultivates the inside of part 100.
Fig. 3 be explanation tubular type bioreactor comprise second cultivate the orthographic plan of an embodiment of part 300.As shown in Figure 3, second cultivate the second culture solution outlet 340 that part 300 is equipped with and the 3rd culture solution of pump part 200 exports 230 the second culture solution imports 310 that are connected, culture tube 330 and is connected with the first culture solution import 131 of the first cultivation part 100.
Secondary light source 320 can be coupled to the local or whole of the second cultivation part 300.For example, secondary light source 320 can be coupled to the outside of culture tube 330, extending longitudinally along described outside.The second effect of cultivating the culture tube 330 of part 300 is that the culture solution that comprises the micro-algae of photosynthesis is circulated, and the light that provides the micro-algae of photosynthesis in circulation to receive from secondary light source 320 carries out photosynthetic environment.Therefore, culture tube 330 can consist of transparent material, to transmit the light sending from secondary light source 330.Culture tube 330 can integral body only consist of transparent material, or is only that the part that light passes through consists of transparent material.Or secondary light source 320 can be placed on the inside of culture tube 330.In this situation, secondary light source 310 is preferably coupled to internal surface and LED (photodiode) preferably, and culture tube 330 can consist of opaque material.In addition, such as pH sensor, CO 2the sensor of sensor, dissolved oxygen sensor, temperature sensor can be coupled to one or more sides of culture tube 330.In addition, culture tube 330 advantageous version are long narrow tubular form, so that be exposed to the area of light, maximize.In this situation, culture tube 330 advantageous version are pleated sheet structure, and secondary light source 320 can repeatedly provide between the pleated sheet structure of culture tube 330.
In one embodiment of the invention, the second culture tube 330 of cultivating part 300 can be configured to parallel repeatedly folded form, be included in straight part and curved part on a support, or this folded form can be piled into multilayered structure.Also have, as comprising in addition the power supply of secondary light source 320 for fixing this support of the shelf of secondary light source 320.In addition, the effect of secondary light source 320 is to emit beam in culturing process, to carry out photosynthesis for the micro-algae of photosynthesis.The wavelength sending from secondary light source 320 is preferably similar to three-wavelength or five wavelength of daylight, but is not limited to these wavelength, and preferably exposure intensity and light-dark cycle is controlled automatically according to culture condition.
Meanwhile, because culture tube 330 is deformed into the repeatedly pleated sheet structure that comprises straight part and curved part, the oxygen producing in cultivating the micro-algae process of photosynthesis can be accumulated in curved part and not be released.In order to force the oxygen of emitting accumulation, preferably culture tube is equipped with oxygen escaper in addition at curved part.When oxygen accumulation surpasses certain level, the oxygen of accumulation is discharged (referring to Fig. 4) by the automatic operation of oxygen escaper.
Fig. 4 is the schematic diagram that an embodiment of the oxygen escaper that is coupled to culture tube 330 is described.As shown in Figure 4, oxygen escaper comprises grounding sensor 351, the mass flow controller 352 that becomes to be electrically connected to grounding sensor, the valve 353 being connected with mass flow controller 352, and one end of grounding sensor 351 is immersed in culture solution to apply electric current.
If because the accumulation of oxygen in curved part formed gas blanket, grounding sensor 351 disengaging culture solution come out, so current interruptions.In interrupt procedure, the valve 353 being connected with mass flow controller 352 actuated, so discharge a certain amount of oxygen.When the discharge grounding sensor along with oxygen is immersed in culture solution again, electric current is applied in, and valve 353 stops.
In yet another aspect, tubular type bioreactor of the present invention can comprise that one is cultivated part and two or more pumps part and culture tube part.
Fig. 5 is the orthographic plan that an embodiment of part is cultivated in explanation, and this cultivation part has two pumps of two use partly provides 132, two of the first culture solution outlets of culture solution flow into the first culture solution import 131 of culture solution and cultivate part 100 from two culture tubes 330.As shown in Figure 5, use and comprise that one is cultivated part, two or more pumps partly and the tubular type bioreactor of culture tube part, cultivate part and can partly provide culture solution to two culture tubes, thereby cultivation can be more efficient.
Hereinafter with reference accompanying drawing is described operation and the effect of tubular type bioreactor in detail.
First, the first culture solution import 131, the first culture solution outlet 132 and the final outlet 133 of the first cultivation part 100 are closed, micro-algae algae kind inoculum is injected to culture tank 101 by inoculum import 110 or fresh culture import 134.Then from the first light source 140, apply light and cultivate for the first time, use temperature controller 160 also starts agitator 150 and maintains suitable temperature.With the various sensors that are coupled to the sensor port 120 of the first cultivation part 100, check after culture condition, at reasonable time, stop first cultivation.
Then, fresh culture is injected to culture tank 101 by fresh culture import 134, and start continuously agitator 150 so that the culture solution after cultivating is for the first time mixed with the fresh culture of injection.When injecting after culture tank 101 with the isopyknic fresh culture of first culture solution, open the first culture solution import 131 and the first culture solution outlet 132, the culture solution that makes to mix is transferred to the 3rd culture solution import 210 of pump part 200.If the culture solution of mixing is transferred to the 3rd culture solution import 210 of pump part 200, the pump 220 of pump part 200 starts, and the culture solution of mixing is transferred to the second culture solution import 310 of the second cultivation part 300 by the 3rd culture solution outlet 230.The mixed culture solution that is transferred to the second culture solution import 310 of the second cultivation part 300 is transferred to the first culture solution import 131 of culture tube 330, second culture solution outlet the 340 and first cultivation part 100 successively by pump 220, and circulation (first cultivation part → pump part → second cultivated partly → first and cultivate part) startup.If loop start, secondary light source 320 emits beam to irradiate culture tube 330, and the micro-algae of photosynthesis contained in culture solution carries out photosynthesis.The flow velocity of culture solution of circulation can be controlled with the flow speed controller that is electrically coupled to pump, with prevent the micro-algae of photosynthesis particularly spirulina occur to attach and make the photosynthesis of the micro-algae in circulation to maximize.Flow velocity can be controlled between 1-50cm/s.More preferably, flow velocity is 10-40cm/s.In the most preferred embodiment, flow velocity is 20-30cm/s, but is not limited to this scope.
In the Cyclic culture process of the micro-algae of photosynthesis, by culture tank 101 injecting carbon dioxides and the nitrogen mixture body to the first cultivation part 100 via gas feed 111, thereby apply about 0.1-1.0kg/cm to culture tank 101 2the malleation of f, can prevent from deriving from the various microbiological contamination of environment, and the pH of culture solution controls by adjusting the dividing potential drop of the carbonic acid gas comprising in mixed gas.
In addition, the oxygen that photosynthesis in the micro-algae culturing process of photosynthesis produces is after culture tank 101 places of the first cultivation part 100 separate from culture solution, be transferred to the top of culture tank 101, then oxygen outwards discharges by pressure controlled valve 112.In this situation, if the second culture tube 330 of cultivating part 200 is configured to be included in straight part on a support and the parallel repeatedly folded form of curved part, oxygen can be accumulated in the curved part of culture tube 330.Therefore, preferably by connect one or more oxygen escapers at curved part, remove the oxygen of accumulation in culture tube 330.
When the Cyclic culture of the micro-algae of photosynthesis stops, the final culture solution that comprises the micro-algae of photosynthesis by opening final outlet 133 results of the first cultivation part 100.After cultivating termination, the inventor has confirmed significant result, and the internal surface of culture tube 330 has 5% by the micro-algae of photosynthesis, to be covered at the most.Therefore, the inventor is by using the light source with culture tube assembling only to apply the rate of propagation that optical condition reduces the micro-algae of photosynthesis, and by making culture solution be partly recirculated to pump, be recycled to the second cultivation part and be partly recirculated to the first cultivation from the second cultivation from pump and partly make the mobility of culture solution maximize from the first cultivation, solved because the micro-algae of photosynthesis is attached at the problem that culture efficiency that the internal surface of culture vessel causes declines.
The micro-algae of photosynthesis can be Chlorella (Chlorella), Dunaliella (Gunaliella), Spirullina (Spirulina), but is not limited to them.More preferably, the micro-algae of photosynthesis is Spirullina.
Below in conjunction with some exemplary, the present invention has been described, but should understand, the present invention is not limited to disclosed embodiment, thinks that on the contrary the present invention covers the various modifications in the spirit and scope that are included in appended claims and equivalent thereof and is equal to arrangement.

Claims (13)

1. a bioreactor, described bioreactor comprises:
The first cultivation part, this first cultivation part comprises the culture tank that wherein substratum is supplied, and is coupled to culture tank to irradiate the first light source of culture tank inside, wherein this first cultivation partly comprises and is coupled to culture tank outside for controlling the water jacket of temperature;
Second cultivates part, this second is cultivated part and comprises that to be arranged on culture tank outside and by the culture tube from culture tank supply culture solution, with be coupled to culture tube to irradiate the secondary light source of culture tube inside, wherein this culture tube has and comprises parallel many folded form of straight part and curved part and comprise a plurality of secondary light sources that are coupled to culture tube outside along culture tube longitudinal direction, and this culture tube is equipped with oxygen escaper at curved part; With
Pump part, this pump part is cultivated part with the first cultivation part and second, and both are connected, so that culture solution partly and between the second cultivation part circulates in the first cultivation,
Wherein said pump comprise can be by the flow rate control of nutrient solution in culture tube the flow speed controller between 10-40cm/s.
2. the bioreactor of claim 1, wherein the first light source is arranged on culture tank inside.
3. the bioreactor of claim 1, wherein first cultivate that part also comprises and culture tank and pump part both at least one first culture solution import and at least one first culture solution outlet of being connected, second cultivates part also comprises respectively and exporting with at least one the second culture solution import being connected with the second culture solution import and at least one second culture solution by pump part and the outlet of the first culture solution.
4. the bioreactor of claim 1, wherein first cultivates part and also comprises for fresh culture being fed to fresh culture import and the final outlet for culture solution is discharged from culture tank of culture tank.
5. the bioreactor of claim 1, wherein first cultivates part and also comprises the agitator for mixed culture solution that is coupled to culture tank inside.
6. the bioreactor of claim 1, wherein first cultivates part and also comprises for controlling the pressure controlled valve of the pressure of culture tank.
7. the bioreactor of claim 1, wherein first cultivates part and also comprises one or more sensor ports that are coupled to culture tank.
8. the bioreactor of claim 1, wherein first cultivates part and also comprises and be coupled to culture tank with by the gas feed of gas inject culture tank.
9. the bioreactor of claim 1, wherein said oxygen escaper comprises grounding sensor, the mass flow controller and the valve being connected with mass flow controller that are electrically connected to grounding sensor.
10. the bioreactor of claim 1, the internal diameter of wherein said culture tube is 3-30cm.
11. 1 kinds of bioreactors, described bioreactor comprises:
First cultivates part, this the first cultivation part comprises culture tank, the first substratum import, the first substratum outlet and the first light source that wherein substratum is supplied, described import, outlet and light source are coupled to culture tank separately, and wherein this first cultivation partly comprises and is coupled to culture tank outside for controlling the water jacket of temperature;
Second cultivates part, this second is cultivated part and comprises that with tubular form, to be arranged in culture tank outside and from culture tube, the second substratum import, second substratum outlet and a plurality of secondary light source of culture tank supplied with medium, described import, outlet and light source are coupled to culture tube along culture tube longitudinal direction separately, wherein this culture tube has and comprises parallel many folded form of straight part and curved part and be equipped with oxygen escaper at curved part, and
Pump part, this pump part is cultivated part with the first cultivation part and second, and both are connected, so that culture solution is cultivated between part and circulated in the first cultivation part and second;
Wherein the first substratum outlet is connected with the second substratum import, and the first substratum import is connected with the second substratum outlet, makes substratum to cultivate between part and to circulate in the first cultivation part and second, and
Wherein said pump partly comprise can be by flow rate control the flow speed controller between 10-40cm/s.
The bioreactor of 12. claims 11, wherein said pump partly comprises:
The 3rd culture solution import between the first culture solution outlet and the second culture solution import; With
The 3rd culture solution outlet between the first culture solution import and the outlet of the second culture solution.
The bioreactor of 13. claims 11, the internal diameter of wherein said culture tube is 3-30cm.
CN201010248859.6A 2010-07-29 2010-07-29 Circulating type photobioreactor Expired - Fee Related CN102344888B (en)

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CN102344889A (en) * 2010-07-29 2012-02-08 凯洛斯环球有限公司 Method for cyclic culture of photosynthetic microalgae
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WO2002086053A1 (en) * 2001-04-19 2002-10-31 Bioprocess A/S Improvements relating to bioreactors
CN101575567A (en) * 2009-06-22 2009-11-11 北京科技大学 Method for culturing microalgae by illumination way and reactor thereof

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