CN104152344A - Photobioreactor used for culturing benthic microalgae and culturing method thereof - Google Patents
Photobioreactor used for culturing benthic microalgae and culturing method thereof Download PDFInfo
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Abstract
The invention discloses a photobioreactor used for culturing benthic microalgae and a culturing method thereof. The photobioreactor comprises a reactor and a liquid inlet/outlet pipeline, wherein in the reactor, the conditions such as a culture solution, lighting, pH and temperature can be adjusted, LED light sources are adopted, and carbon dioxide is continuously supplemented, so that microalgae can be subjected to long-time illumination during the suspension process to be greatly promoted to perform photosynthesis, so that various single benthic microalgae species can be cultured. According to the invention, the pollution from other external algae and protozoa is avoided by virtue of a stable culturing environment and a closed culturing system; the equipment structure is simple, the luminous energy utilization ratio is high, the culture and harvest are simple and convenient, and the large-scale industrial production can be implemented.
Description
Technical field
The present invention relates to the end micro-algae technical field of dwelling, particularly relate to a kind of for cultivating the end dwell bioreactor and the cultural method thereof of micro-algae.
Background technology
Micro-algae that dwells is of a great variety at the end, in its frustule, generally contain abundant biologically active substance, mineral substance and trace element, as vitamin A, vitamins C, vitamin-E, vitamin B12, thiamine, riboflavin, pyridoxol vitamin H, inositol, folic acid, calcium pantothenate and nicotinic acid etc., the unsaturated fatty acids such as high yield DHA (docosahexenoic acid), EPA (timnodonic acid) in micro-algae that dwells at the bottom of part.Along with the end dwell micro-algae research further deeply, it,, in modern food, medicine and high-grade fishery products are cultivated, more and more occupies an important position.
Micro-algae is dwelt under physical environment in the end, and general distribution is grown in water bottom, accepts natural lighting deficiency, the efficiency of light energy utilization is low, water body CO
2content is few, makes its photosynthetic ability very limited, and therefore its speed of growth is extremely slow.Micro-algae that dwells is applied to seed rearing stage of aquaculture in early days at the end, traditional cultural method is to utilize open cement pit, its production efficiency is low, operation inconvenience, cost of labor are high, in culturing process very easily by assorted algae and the aquatic biological pollution of external source, this also becomes the end greatest problem that the single algae kind of micro-algae high-density culture faces of dwelling.Because early stage people are few to the dwell commercial value understanding of micro-algae of the end, micro-algae that dwells of traditional end is cultivated and is mainly used in aquatic products shellfish culture and the breeding of growing seedlings in early days, and it applies the restriction with regional and seasonal feature, thereby output is less.The culture device using in conventional art is also very simple, has plastics bag, cement pit, plastic bucket.The end dwell micro-algae in culturing process often deposition in water bottom, even by stirring or put into gas stone, also can only make micro-algae that dwells of the local end suspend, also can there is dead angle.The series of problems that traditional way exists, has limited the end highdensity batch production of the single algae kind of micro-algae of dwelling and has produced.Raw material lack the serious application of micro-algae resource in fields such as medicine, food of dwelling of the abundant end that restricted.
Summary of the invention
The object of this invention is to provide a kind ofly for cultivating the end dwell bioreactor and the cultural method thereof of micro-algae, can be used for producing micro-algae that dwells of the different ends, carry out mass-producing ground and produce continuously to realize factory, make up the deficiencies in the prior art.
For realizing the technical scheme that object of the present invention adopts be: a kind of for cultivating the dwell bioreactor of micro-algae of the end, it is characterized in that comprising reactor and liquid in-out pipeline; Described reactor comprises the middle cylinder of at least one hollow structure, and the top bolt of described middle cylinder is connected with top cover, and its bottom end seal has bottom; Described top cover has top cover venting hole and an inlet opening; Described liquid in-out pipeline comprises feed liquor main road, and described feed liquor main road is connected with the feed liquor branch road that quantity is consistent with described middle cylinder quantity, and described feed liquor branch road is connected with the inlet opening on described top cover, is provided with feed liquor branch road valve on described feed liquor branch road; The circle centre position of described bottom is provided with a fluid hole, and described fluid hole is connected with fluid branch road; Described fluid branch road is connected with fluid main road, and described fluid main road is connected to algae liquid holding tank; Described bottom is provided with multiple air inlet ports at the periphery of described fluid hole; Below described air inlet port, be connected with admission passage; In the middle of described, the arranged outside of cylinder has LED fluorescent tube, and described LED fluorescent tube is fixedly connected with the outer wall of described middle cylinder by support; Described LED fluorescent tube is connected with LED power rearrangeable switch; In the middle of described, the inside of cylinder is provided with pH detecting electrode and temperature sensor.
Further, described bottom is provided with the bend pipe of giving vent to anger on described air inlet port, described in the bend pipe of giving vent to anger be communicated with described admission passage.
Preferably, described in the bend pipe of giving vent to anger formed by vertical portion and bend, the angle that vertical portion and bend form is 45 °.
Preferably, the angle that the wire of the projection of described bend on described bottom and described vertical portion and described fluid hole forms is 45 °.
Apply above-mentioned bioreactor and cultivate the dwell method of micro-algae of the end, it is characterized in that comprising the following steps:
(1) steam sterilizing
Before bioreactor operation, need to carry out high-temperature steam sterilization; First close top cover venting hole, fluid branch road valve, admission passage, open feed liquor branch road valve, fluid branch road valve; High-temperature steam is entered by fluid main road, then is discharged by feed liquor main road;
(2) dress liquid
Close fluid branch road valve, open top cover venting hole and with absorbent cotton sealing, will after the mixed gas filtration of carbonic acid gas and air, be passed into the inside of middle cylinder by admission passage; Subsequently elementary algae seedling solution is flowed into by feed liquor main road, then cylinder in the middle of elementary algae seedling solution being injected by feed liquor branch road respectively; After elementary algae seedling solution feed liquor completes, injection nutrient solution uses the same method;
(3) illumination
After intermediolateral column body dress liquid, check whether feed liquor branch road valve closes completely; Open LED power rearrangeable switch, intermediolateral column body is carried out to continuous light; Monitor by pH detecting electrode and temperature sensor, in maintenance culturing process, pH scope is between 7.5～10.5, and temperature is controlled between 25～40 DEG C;
(4) cultivate
Incubation time is 5～10 days, when micro-algae number reaches 2*10
5when individual/milliliter is above, gather; In recovery process, keep admission passage to continue air inlet, open fluid branch road valve, the algae liquid in the middle of making in cylinder enters algae liquid holding tank by fluid main road;
(5) continuity is cultivated
In the time that the residue algae liquid of each middle cylinder is 20～30L, close fluid branch road valve, open feed liquor branch road valve, inject nutrient solution by feed liquor branch road respectively by feed liquor main road, again carry out step (2) and (3), cultivate to realize continuity; Continuity incubation times is controlled at 3～5 times, while again cultivation, need to carry out the steam sterilizing operation of step (1).
Preferably, in described step (2), the volume ratio of the mixed gas of carbonic acid gas and air is V
carbonic acid gas: V
air=2%～5%.
Preferably, the liquid measure that in described step (2), each middle cylinder injects elementary algae seedling solution is 20～30L.
Preferably, the liquid measure that in described step (2), each middle cylinder injects nutrient solution is 80～90L.
Preferably, the liquid measure that in described step (5), each middle cylinder injects nutrient solution is 80～90L.
Compared with prior art, the invention has the beneficial effects as follows:
(1) present device is simple in structure, easy to operate, the efficiency of light energy utilization is high, cultivate and gather easyly, can realize large-scale industrialized production;
(2), by the end microalgae photobiological reactor of dwelling, can micro-algae that dwells of the different ends be realized differentiation and produced, to nutritive salt required in culturing process, CO
2supply, pH environment rationally regulate, and strictly control outside contamination source, realize the end dwell the single algae kind of micro-algae high-density batch production produce;
(3) the present invention can pass through the condition such as nutrient solution, illumination, pH, temperature and regulates, and realizes and cultivates the algae kind of dwelling of the different single ends; Stable culture environment and the culture systems of sealing have been avoided extraneous assorted algae and protozoic pollution; Airlift aeration device has been avoided the dwell sedimentation phenomenon of micro-algae of the end, is coordinating constantly supplementing of LED light source and carbonic acid gas, makes micro-algae time length in the process suspending accept illumination, greatly promotes it to carry out photosynthesis.
Brief description of the drawings
Figure 1 shows that the structural representation of bioreactor of the present invention;
Figure 2 shows that bottom arranges the perspective view of four bend pipes of giving vent to anger;
Figure 3 shows that the plane structure chart of the bend pipe of giving vent to anger in Fig. 2;
Figure 4 shows that the vertical view of Fig. 2.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in further detail.Should be appreciated that specific embodiment described herein, only in order to explain the present invention, is not intended to limit the present invention.
Embodiment:
Be illustrated in figure 1 a kind ofly for cultivating the dwell bioreactor of micro-algae of the end, it is characterized in that comprising reactor and liquid in-out pipeline; Described reactor comprises the middle cylinder 12 of at least one hollow structure; Shown in Fig. 1, be cylinder 12 in the middle of four, in the suitability for industrialized production stage, according to production-scale needs, the quantity of cylinder 12 in the middle of can adding or reduce; In the middle of described, the internal diameter of cylinder 12 is 350mm, and wall thickness 10mm, is highly 1500mm, adopts transparent acrylic material to make, and the top bolt of middle cylinder 12 is connected with top cover 8, and its bottom is welded with bottom 10; The edge average mark of described top cover 8 is furnished with the hole of six 10mm diameters, for being bolted with middle cylinder 12; Described top cover 8 has top cover venting hole 9; Top cover venting hole 9 adopts internal diameter 10mm, external diameter 2mm, long 30mm acrylic open tube, is welded on top cover 8, plays scavenging action.
The diameter of described bottom 10 is 400mm, and wall thickness is 10mm, adopts acrylic material, is evenly distributed the bolt connecting hole 22 of six 10mm diameters at the edge of bottom 10, and bolt connecting hole 22 is for being bolted with supporting platform 20.
Preferably, described bottom 10 is filled with silicagel pad 13 with described supporting platform 20 is middle, and the thickness of silicagel pad 13 is 10mm, and its size is identical with bottom 10, carries out borehole so that bolt passes in the position of described bolt connecting hole 22 correspondences.
Described liquid in-out pipeline comprises feed liquor main road 1, described feed liquor main road 1 is connected with the feed liquor branch road 3 that quantity is consistent with described middle cylinder quantity, and described feed liquor branch road 3 is connected (on described feed liquor branch road 3, being provided with feed liquor branch road valve 2) with the inlet opening on described top cover 8; The circle centre position of described bottom 10 is provided with the fluid hole 4 that diameter is 55mm, and described fluid hole 4 is connected with fluid branch road 5; Described fluid branch road 5 is connected with fluid main road 7, and described fluid main road 7 is connected to algae liquid holding tank 21; Wherein, it is the PVC water shoot of 40mm that fluid branch road 5 adopts internal diameter, is provided with fluid branch road valve 6 on fluid branch road 5, and it is the PVC water shoot of 50mm that fluid main road 7 adopts internal diameter.
The periphery of described fluid hole 4 is provided with multiple air inlet ports 23; The lower end of described air inlet port 23 is connected with admission passage 11; The specification of admission passage 11 is internal diameter 10mm, external diameter 2mm, long 30mm, and material is acrylic.
In the middle of described, a side of cylinder 12 is provided with LED fluorescent tube 15, the diameter 30mm of LED fluorescent tube 15, long 1200mm, power 26W, described LED fluorescent tube 15 is fixedly connected with described middle cylinder 12 with lower bracket 16 by upper bracket 14, upper bracket 14 and lower bracket 16 all adopt acrylic material to make, by being welded and fixed with the outer wall of middle cylinder 12; On described supporting platform 20, be provided with the LED power rearrangeable switch 17 being connected with LED fluorescent tube 15.
In the middle of described, the inside of cylinder 12 is provided with pH detecting electrode 18 and temperature sensor 19; Bioreactor of the present invention is in the time of operation, and pH detecting electrode 18 and temperature sensor 19 need to submerge in nutrient solution completely, so that collect related data.
Further, as shown in Figure 2, the upper end of described air inlet port 23 is provided with the bend pipe 24 of giving vent to anger, described in the bend pipe 24 of giving vent to anger be communicated with described admission passage 11.
Preferably, as shown in Figure 3, described in the bend pipe 24 of giving vent to anger formed by vertical portion 241 and bend 242, the angle b that vertical portion 241 and bend 242 form is 45 °.
Preferably, as shown in Figure 4, projection and the described vertical portion 241 of described bend 242 on described bottom 10 is 45 ° with the angle a that the wire of described fluid hole 4 forms.
Above-mentioned known, gas that described admission passage 11 passes into is discharged through the bend pipe 24 of giving vent to anger, and because bottom 10 be circular, the air-flow that four bend pipes 24 of giving vent to anger form can carry out without dead angle impact bottom 10, and the interior formation eddy current of middle cylinder 12; In the middle of being deposited in, micro-algae that dwells is followed eddy current and rises at the end of cylinder 12 bottoms, make the end dwell micro-algae can be suspended in the middle of in nutrient solution in cylinder 12, under kinestate, accept the irradiation of LED light source, make Airlift aeration effect better.
Apply above-mentioned bioreactor and cultivate the dwell method of micro-algae of the end, it is characterized in that comprising the following steps:
(1) steam sterilizing
Before bioreactor operation, need to carry out high-temperature steam sterilization; First close top cover venting hole 9, fluid branch road valve 6, admission passage 11, open feed liquor branch road valve 2, fluid branch road valve 6.High-temperature steam is entered by fluid main road 7, is discharged by feed liquor main road 1, and the process of steam sterilizing continues one hour, to realize the sterilization in bioreactor.
(2) dress liquid
Close fluid branch road valve 6, open top cover venting hole 9 and seal with absorbent cotton, after being filtered, the mixed gas of carbonic acid gas and air enters bioreactor by admission passage 11, realize Airlift aeration function, make the end dwell micro-algae in culturing process, constantly rolling and sedimentation can not occur in chamber; Now elementary algae seedling solution is flowed into by feed liquor main road 1, then cylinder 12 in the middle of elementary algae seedling solution being injected by feed liquor branch road 3 respectively; After middle cylinder 12 feed liquors complete, re-injected the nutrient solution preparing by feed liquor branch road 3 by feed liquor main road 1;
(3) illumination
After intermediolateral column body 12 fills liquid, check whether feed liquor branch road valve 2 closes completely; Open LED power rearrangeable switch 17, realize continuous light, ensure photosynthesis stable carrying out smoothly; By pH detecting electrode 18 and temperature sensor 19 Real-Time Monitorings, in maintenance culturing process, pH scope is between 7.5～10.5, and temperature is controlled between 25～40 DEG C; PH value can be regulated by carbonic acid gas intake, and temperature can be regulated by room conditioning.
(4) cultivate
Incubation time is 5～10 days, when micro-algae number reaches 2*10
5when individual/milliliter is above, gather; In recovery process, keep admission passage 11 to continue air inlet, open fluid branch road valve 6, make the algae liquid in culture chamber evenly enter algae liquid holding tank 21 by fluid main road 7.
(5) continuity is cultivated
In the time that the remaining algae liquid of each middle cylinder 12 is 20～30L, close fluid branch road valve 6, open feed liquor branch road valve 2, inject by feed liquor branch road 3 nutrient solution preparing respectively by feed liquor main road 1, realize continuity and cultivate; Continuity incubation times is controlled at 3～5 times, while again cultivation, need to carry out to bioreactor the steam sterilizing operation of step (1).
Preferably, in described step (2), the volume ratio of the mixed gas of carbonic acid gas and air is V
carbonic acid gas: V
air=2%～5%, its object is to make up the deficiency of carbonic acid gas in culturing process.
Preferably, the liquid measure that in described step (2), each middle cylinder 12 injects elementary algae seedling solution is 20～30L.
Preferably, the liquid measure that in described step (2), each middle cylinder 12 injects nutrient solution is 80～90L.
Preferably, the liquid measure that in described step (5), each middle cylinder 12 injects nutrient solution is 80～90L
Inject in above-mentioned steps (2) and step (5) for cultivating the dwell nutrient solution of micro-algae of the end, the concentration range of each component is respectively: NaNO
3: 17.0～13.0g/L, K
2hPO
4: 4.0～1.0g/L, MgSO
47H
2o:4.0～2.0g/L, CaC
122H
2o:2.0～1.0g/L, citric acid: 0.5～0.1g/L, ferric ammonium citrate: 0.5～0.1g/L, EDTANa
2: 0.1～0.3g/L, Na
2cO
3: 2.0～0.5g/L, H
3bO
3: 3.5～1.5g/L, MnC
124H
2o:3.0～1.0g/L, ZnSO
47H
2o:0.8～0.1g/L, Na
2moO
42H
2o:0.8～0.1g/L, CuSO
45H
2o:0.2～0.05g/L, Co (NO
3)
26H
2o:0.1～0.02g/L.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (9)
1. for cultivating the dwell bioreactor of micro-algae of the end, it is characterized in that comprising reactor and liquid in-out pipeline; Described reactor comprises the middle cylinder of at least one hollow structure, and the top bolt of described middle cylinder is connected with top cover, and its bottom end seal has bottom; Described top cover has top cover venting hole and an inlet opening; Described liquid in-out pipeline comprises feed liquor main road, and described feed liquor main road is connected with the feed liquor branch road that quantity is consistent with described middle cylinder quantity, and described feed liquor branch road is connected with the inlet opening on described top cover, is provided with feed liquor branch road valve on described feed liquor branch road; The circle centre position of described bottom is provided with a fluid hole, and described fluid hole is connected with fluid branch road; Described fluid branch road is connected with fluid main road, and described fluid main road is connected to algae liquid holding tank; Described bottom is provided with multiple air inlet ports at the periphery of described fluid hole; Below described air inlet port, be connected with admission passage; In the middle of described, the arranged outside of cylinder has LED fluorescent tube, and described LED fluorescent tube is fixedly connected with the outer wall of described middle cylinder by support; Described LED fluorescent tube is connected with LED power rearrangeable switch; In the middle of described, the inside of cylinder is provided with pH detecting electrode and temperature sensor.
2. according to claim 1ly a kind ofly it is characterized in that for cultivating the dwell bioreactor of micro-algae of the end described bottom is provided with the bend pipe of giving vent to anger on described air inlet port, described in the bend pipe of giving vent to anger be communicated with described admission passage.
3. according to claim 1 a kind of for cultivating the dwell bioreactor of micro-algae of the end, the bend pipe of giving vent to anger described in it is characterized in that is made up of vertical portion and bend, and the angle that vertical portion and bend form is 45 °.
4. according to claim 1 a kind of for cultivating the dwell bioreactor of micro-algae of the end, it is characterized in that the angle that the wire of the projection of described bend on described bottom and described vertical portion and described fluid hole forms is 45 °.
5. application rights requires the described bioreactor of one of 1-4 to cultivate the dwell method of micro-algae of the end, it is characterized in that comprising the following steps:
(1) steam sterilizing
Before bioreactor operation, need to carry out high-temperature steam sterilization; First close top cover venting hole, fluid branch road valve, admission passage, open feed liquor branch road valve, fluid branch road valve; High-temperature steam is entered by fluid main road, then is discharged by feed liquor main road;
(2) dress liquid
Close fluid branch road valve, open top cover venting hole and with absorbent cotton sealing, will after the mixed gas filtration of carbonic acid gas and air, be passed into the inside of middle cylinder by admission passage; Subsequently elementary algae seedling solution is flowed into by feed liquor main road, then cylinder in the middle of elementary algae seedling solution being injected by feed liquor branch road respectively; After elementary algae seedling solution feed liquor completes, injection nutrient solution uses the same method;
(3) illumination
After intermediolateral column body dress liquid, check whether feed liquor branch road valve closes completely; Open LED power rearrangeable switch, intermediolateral column body is carried out to continuous light; Monitor by pH detecting electrode and temperature sensor, in maintenance culturing process, pH scope is between 7.5～10.5, and temperature is controlled between 25～40 DEG C;
(4) cultivate
Incubation time is 5～10 days, when micro-algae number reaches 2*10
5when individual/milliliter is above, gather; In recovery process, keep admission passage to continue air inlet, open fluid branch road valve, the algae liquid in the middle of making in cylinder enters algae liquid holding tank by fluid main road;
(5) continuity is cultivated
In the time that the residue algae liquid of each middle cylinder is 20～30L, close fluid branch road valve, open feed liquor branch road valve, inject nutrient solution by feed liquor branch road respectively by feed liquor main road, again carry out step (2) and (3), cultivate to realize continuity; Continuity incubation times is controlled at 3～5 times, while again cultivation, need to carry out the steam sterilizing operation of step (1).
6. bioreactor according to claim 5 is cultivated the dwell method of micro-algae of the end, it is characterized in that the volume ratio of the mixed gas of carbonic acid gas and air in described step (2) is V
carbonic acid gas: V
air=2%～5%.
7. bioreactor according to claim 5 is cultivated the dwell method of micro-algae of the end, it is characterized in that each in described step (2) in the middle of the cylinder liquid measure of injecting elementary algae seedling solution be 20～30L.
8. bioreactor according to claim 5 is cultivated the dwell method of micro-algae of the end, it is characterized in that each in described step (2) in the middle of the cylinder liquid measure of injecting nutrient solution be 80～90L.
9. bioreactor according to claim 5 is cultivated the dwell method of micro-algae of the end, it is characterized in that each in described step (5) in the middle of the cylinder liquid measure of injecting nutrient solution be 80～90L.
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