CN204298406U - A kind of photo-biological reactor realizing continuity and cultivate - Google Patents
A kind of photo-biological reactor realizing continuity and cultivate Download PDFInfo
- Publication number
- CN204298406U CN204298406U CN201420435048.0U CN201420435048U CN204298406U CN 204298406 U CN204298406 U CN 204298406U CN 201420435048 U CN201420435048 U CN 201420435048U CN 204298406 U CN204298406 U CN 204298406U
- Authority
- CN
- China
- Prior art keywords
- intermediate cylinder
- feed liquor
- branch road
- top cover
- photo
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The utility model discloses a kind of photo-biological reactor realizing continuity and cultivate, it comprises reactor and liquid in-out pipeline; Described reactor comprises the intermediate cylinder of at least one hollow structure, and the top bolt of described intermediate cylinder is connected with top cover, and its bottom end seal has bottom; Described top cover has top cover venting hole and an inlet opening; The arranged outside of described intermediate cylinder has LED lamp tube, and described LED lamp tube is fixedly connected with by the outer wall of support with described intermediate cylinder; Described LED lamp tube is connected with LED power adjustable switch; The inside of described intermediate cylinder is provided with pH detecting electrode and temperature sensor.The utility model device structure is simple, and the efficiency of light energy utilization is high, cultivates and gathers easy, can realize large-scale industrialized production.
Description
Technical field
The utility model relates to the end and to dwell micro-algae technical field, particularly relates to a kind of photo-biological reactor realizing continuity and cultivate.
Background technology
Micro-algae that dwells is of a great variety at the end, generally containing abundant biologically active substance, mineral substance and trace element in its frustule, as vitamin A, vitamins C, vitamin-E, vitamin B12, thiamine, riboflavin, pyridoxol vitamin H, inositol, folic acid, calcium pantothenate and nicotinic acid etc., the unsaturated fatty acids such as high yield DHA (docosahexenoic acid), EPA (timnodonic acid) in micro-algae that dwells at the bottom of part.Along with the end dwell micro-algae research further deeply, it, in modern food, medicine and high-grade fishery products are cultivated, more and more occupies an important position.
Micro-algae is dwelt under physical environment in the end, and general distribution growth, in water bottom, accepts natural lighting deficiency, the efficiency of light energy utilization is low, water body CO
2content is few, and make its photosynthetic ability very limited, therefore its speed of growth is extremely slow.Micro-algae that dwells is applied to seed rearing stage of aquaculture in early days at the end, traditional cultural method utilizes open cement pit, its production efficiency is low, operation inconvenience, cost of labor are high, in culturing process very easily by assorted algae and the aquatic biological pollution of external source, this also becomes the end and to dwell the greatest problem that micro-algae single algae kind high-density culture faces.Because early stage people are few to the dwell commercial value understanding of micro-algae of the end, microdisk electrode of dwelling of traditional end is mainly used in aquatic products shellfish culture and early stage nursery breeding, and it applies the restriction with regional and seasonal feature, and thus output is less.The culture device used in conventional art is also very simple, has plastics bag, cement pit, plastic bucket.The end dwell micro-algae in culturing process frequent deposition in water bottom, even if by stirring or put into gas stone, also can only make local the end dwell micro-algae suspend, also can there is dead angle.The series of problems that traditional way exists, limiting the end dwells the highdensity factorial praluction of micro-algae single algae kind.The lacking of raw material, seriously constrains the abundant end and to dwell the application of micro-algae resource in fields such as medicine, food.
Utility model content
The purpose of this utility model is to provide a kind of photo-biological reactor realizing continuity and cultivate, and can be used for producing the different ends to dwell micro-algae, carries out mass-producing ground continuous seepage, make up the deficiencies in the prior art to realize factory.
The technical scheme adopted for realizing the purpose of this utility model is: a kind of photo-biological reactor realizing continuity and cultivate, is characterized in that comprising reactor and liquid in-out pipeline; Described reactor comprises the intermediate cylinder of at least one hollow structure, and the top bolt of described intermediate cylinder is connected with top cover, and its bottom end seal has bottom; Described top cover has top cover venting hole and an inlet opening; Described liquid in-out pipeline comprises feed liquor main road, and described feed liquor main road is connected with the quantity feed liquor branch road consistent with described intermediate cylinder quantity, and described feed liquor branch road is connected with the inlet opening on described top cover, described feed liquor branch road is provided with feed liquor branch road valve; The circle centre position of described bottom is provided with a fluid hole, and described fluid hole is connected with fluid branch road; Described fluid branch road is connected with fluid main road, and described fluid main road is connected to algae liquid holding tank; Described bottom is provided with multiple air inlet port at the periphery of described fluid hole; Admission passage is connected with below described air inlet port; The arranged outside of described intermediate cylinder has LED lamp tube, and described LED lamp tube is fixedly connected with by the outer wall of support with described intermediate cylinder; Described LED lamp tube is connected with LED power adjustable switch; The inside of described intermediate cylinder is provided with pH detecting electrode and temperature sensor.
Further, described bottom is provided with bend pipe of giving vent to anger on described air inlet port, described in bend pipe of giving vent to anger be communicated with described admission passage.
Preferably, described in bend pipe of giving vent to anger be made up of vertical portion and bend, the angle that vertical portion and bend are formed is 45 °.
Preferably, the angle that the wire of the projection of described bend on described bottom and described vertical portion and described fluid hole is formed is 45 °.
Apply above-mentioned photo-biological reactor to cultivate the end and to dwell the method for micro-algae, it is characterized in that comprising the following steps:
(1) steam sterilizing
Before photo-biological reactor runs, need to carry out high-temperature steam sterilization; First close top cover venting hole, fluid branch road valve, admission passage, open feed liquor branch road valve, fluid branch road valve; High-temperature steam is entered by fluid main road, then is discharged by feed liquor main road;
(2) liquid is filled
Close fluid branch road valve, open top cover venting hole and with absorbent cotton sealing, after being filtered by the mixed gas of carbonic acid gas and air, passed into the inside of intermediate cylinder by admission passage; Subsequently elementary algae seedling solution is flowed into by feed liquor main road, then respectively by feed liquor branch road, elementary algae seedling solution is injected intermediate cylinder; After elementary algae seedling solution feed liquor completes, use the same method injection nutrient solution;
(3) illumination
After intermediate cylinder dress liquid, check whether feed liquor branch road valve closes completely; Open LED power adjustable switch, continuous light is carried out to intermediate cylinder; Monitored by pH detecting electrode and temperature sensor, in maintenance culturing process, pH scope is between 7.5 ~ 10.5, and temperature controls between 25 ~ 40 DEG C;
(4) cultivate
Incubation time is 5 ~ 10 days, when micro-algae number reaches 2*10
5time more than individual/milliliter, gather; In recovery process, keep admission passage to continue air inlet, open fluid branch road valve, make the algae liquid in intermediate cylinder enter algae liquid holding tank by fluid main road;
(5) continuity is cultivated
When the residue algae liquid of each intermediate cylinder is 20 ~ 30L, close fluid branch road valve, open feed liquor branch road valve, inject nutrient solution by feed liquor main road respectively by feed liquor branch road, again carry out step (2) and (3), cultivate to realize continuity; Continuity incubation times controls at 3 ~ 5 times, when again cultivating, needs the steam sterilization process carrying out step (1).
Preferably, in described step (2), the volume ratio of the mixed gas of carbonic acid gas and air is V
carbonic acid gas: V
air=2% ~ 5%.
Preferably, the liquid measure that in described step (2), each intermediate cylinder injects elementary algae seedling solution is 20 ~ 30L.
Preferably, in described step (2), the liquid measure of each intermediate cylinder injection nutrient solution is 80 ~ 90L.
Preferably, in described step (5), the liquid measure of each intermediate cylinder injection nutrient solution is 80 ~ 90L.
Compared with prior art, the beneficial effects of the utility model are:
(1) the utility model device structure is simple, easy to operate, the efficiency of light energy utilization is high, cultivate and gather easy, can realize large-scale industrialized production;
(2) to be dwelt microalgae photobiological reaction unit the end of by, micro-algae that can dwell to the different ends realizes differentiation and produces, to nutritive salt, CO required in culturing process
2reasonable adjusting is carried out in supply, pH environment, strict controls outside contamination source, realizes the end to dwell the high-density factorial praluction of the single algae kind of micro-algae;
(3) the utility model can pass through the condition such as nutrient solution, illumination, pH, temperature and regulates, and realizes cultivating the different single ends to dwell algae kind; Stable culture environment and the culture systems closed avoid extraneous assorted algae and protozoic pollution; Airlift aeration device avoids the end and to dwell the sedimentation phenomenon of micro-algae, is combined with constantly supplementing of LED light source and carbonic acid gas, makes micro-algae time length in the process suspended accept illumination, greatly promote that it carries out photosynthesis.
Accompanying drawing explanation
Figure 1 shows that the structural representation of the utility model photo-biological reactor;
Figure 2 shows that bottom arranges the perspective view of four bend pipes of giving vent to anger;
Figure 3 shows that in Fig. 2 the plane structure chart of bend pipe of giving vent to anger;
Figure 4 shows that the vertical view of Fig. 2.
Embodiment
Below in conjunction with specific embodiment, the utility model is described in further detail.Should be appreciated that specific embodiment described herein only in order to explain the utility model, and be not used in restriction the utility model.
Embodiment:
Be illustrated in figure 1 a kind of photo-biological reactor realizing continuity and cultivate, it is characterized in that comprising reactor and liquid in-out pipeline; Described reactor comprises the intermediate cylinder 12 of at least one hollow structure; Be four intermediate cylinder 12 shown in Fig. 1, in the suitability for industrialized production stage, according to production-scale needs, can add or reduce the quantity of intermediate cylinder 12; The internal diameter of described intermediate cylinder 12 is 350mm, and wall thickness 10mm is highly 1500mm, and adopt transparent acrylic material to make, the top bolt of intermediate cylinder 12 is connected with top cover 8, and its bottom is welded with bottom 10; The edge average mark of described top cover 8 is furnished with the hole of six 10mm diameters, for being bolted with intermediate cylinder 12; Described top cover 8 has top cover venting hole 9; Top cover venting hole 9 adopts internal diameter 10mm, external diameter 2mm, long 30mm acrylic open tube, is welded on top cover 8, plays scavenging action.
The diameter of described bottom 10 is 400mm, and wall thickness is 10mm, and adopt acrylic material, be evenly distributed the bolt connecting hole 22 of six 10mm diameters at the edge of bottom 10, bolt connecting hole 22 is for being bolted with supporting platform 20.
Preferably, described bottom 10 has silicagel pad 13 with described supporting platform 20 central filler, and the thickness of silicagel pad 13 is 10mm, and its size is identical with bottom 10, carries out borehole so that bolt passes in the position of described bolt connecting hole 22 correspondence.
Described liquid in-out pipeline comprises feed liquor main road 1, described feed liquor main road 1 is connected with the quantity feed liquor branch road 3 consistent with described intermediate cylinder quantity, and described feed liquor branch road 3 is connected (described feed liquor branch road 3 being provided with feed liquor branch road valve 2) with the inlet opening on described top cover 8; The circle centre position of described bottom 10 is provided with the fluid hole 4 that diameter is 55mm, and described fluid hole 4 is connected with fluid branch road 5; Described fluid branch road 5 is connected with fluid main road 7, and described fluid main road 7 is connected to algae liquid holding tank 21; Wherein, fluid branch road 5 adopts internal diameter to be the PVC drain pipe of 40mm, fluid branch road 5 is provided with fluid branch road valve 6, and fluid main road 7 adopts internal diameter to be the PVC drain pipe of 50mm.
The periphery of described fluid hole 4 is provided with multiple air inlet port 23; The lower end of described air inlet port 23 is connected with admission passage 11; The specification of admission passage 11 is internal diameter 10mm, external diameter 2mm, long 30mm, and material is acrylic.
The side of described intermediate cylinder 12 is provided with LED lamp tube 15, the diameter 30mm of LED lamp tube 15, long 1200mm, power 26W, described LED lamp tube 15 is fixedly connected with described intermediate cylinder 12 with lower bracket 16 by upper bracket 14, upper bracket 14 and lower bracket 16 all adopt acrylic material to make, by being welded and fixed with the outer wall of intermediate cylinder 12; Described supporting platform 20 is provided with the LED power adjustable switch 17 be connected with LED lamp tube 15.
The inside of described intermediate cylinder 12 is provided with pH detecting electrode 18 and temperature sensor 19; Operationally, pH detecting electrode 18 and temperature sensor 19 need to submerge in nutrient solution completely the utility model photo-biological reactor, so that collect related data.
Further, as shown in Figure 2, the upper end of described air inlet port 23 is provided with bend pipe 24 of giving vent to anger, described in bend pipe 24 of giving vent to anger be communicated with described admission passage 11.
Preferably, as shown in Figure 3, described in bend pipe 24 of giving vent to anger be made up of vertical portion 241 and bend 242, the angle b that vertical portion 241 and bend 242 are formed is 45 °.
Preferably, as shown in Figure 4, the angle a that the projection of described bend 242 on described bottom 10 and described vertical portion 241 and the wire of described fluid hole 4 are formed is 45 °.
Above-mentioned known, gas that described admission passage 11 passes into is discharged through bend pipe 24 of giving vent to anger, and because bottom 10 be circular, the air-flow that four bend pipes 24 of giving vent to anger are formed can carry out impacting without dead angle to bottom 10, and the interior formation eddy current of intermediate cylinder 12; Micro-algae that dwells is followed eddy current and rises at the end be deposited in bottom intermediate cylinder 12, in dwelt in end nutrient solution that micro-algae is suspended in intermediate cylinder 12, accepts the irradiation of LED light source, make Airlift aeration effect better under kinestate.
Apply above-mentioned photo-biological reactor to cultivate the end and to dwell the method for micro-algae, it is characterized in that comprising the following steps:
(1) steam sterilizing
Before photo-biological reactor runs, need to carry out high-temperature steam sterilization; First close top cover venting hole 9, fluid branch road valve 6, admission passage 11, open feed liquor branch road valve 2, fluid branch road valve 6.High-temperature steam is entered by fluid main road 7, is discharged by feed liquor main road 1, the process lasts of steam sterilizing one hour, to realize the sterilization in photo-biological reactor.
(2) liquid is filled
Close fluid branch road valve 6, open top cover venting hole 9 and seal with absorbent cotton, photo-biological reactor is entered by admission passage 11 after being filtered by the mixed gas of carbonic acid gas and air, realize Airlift aeration function, namely make the end dwell micro-algae in culturing process, constantly roll in chamber and sedimentation can not occur; Now elementary algae seedling solution is flowed into by feed liquor main road 1, then respectively by feed liquor branch road 3, elementary algae seedling solution is injected intermediate cylinder 12; After intermediate cylinder 12 feed liquor completes, re-injected the nutrient solution prepared by feed liquor branch road 3 by feed liquor main road 1;
(3) illumination
After intermediate cylinder 12 fills liquid, check whether feed liquor branch road valve 2 closes completely; Open LED power adjustable switch 17, realize continuous light, ensure that photosynthesis is stable smoothly and carry out; By pH detecting electrode 18 and temperature sensor 19 Real-Time Monitoring, in maintenance culturing process, pH scope is between 7.5 ~ 10.5, and temperature controls between 25 ~ 40 DEG C; PH value can be regulated by carbonic acid gas intake, and temperature can be regulated by room conditioning.
(4) cultivate
Incubation time is 5 ~ 10 days, when micro-algae number reaches 2*10
5time more than individual/milliliter, gather; In recovery process, keep admission passage 11 to continue air inlet, open fluid branch road valve 6, make the algae liquid in culture chamber evenly enter algae liquid holding tank 21 by fluid main road 7.
(5) continuity is cultivated
When the remaining algae liquid of each intermediate cylinder 12 is 20 ~ 30L, closes fluid branch road valve 6, open feed liquor branch road valve 2, injected the nutrient solution prepared by feed liquor main road 1 respectively by feed liquor branch road 3, realize continuity and cultivate; Continuity incubation times controls at 3 ~ 5 times, when again cultivating, needs steam sterilizing operation photo-biological reactor being carried out to step (1).
Preferably, in described step (2), the volume ratio of the mixed gas of carbonic acid gas and air is V
carbonic acid gas: V
air=2% ~ 5%, its object is to make up the deficiency of carbonic acid gas in culturing process.
Preferably, the liquid measure that in described step (2), each intermediate cylinder 12 injects elementary algae seedling solution is 20 ~ 30L.
Preferably, the liquid measure that in described step (2), each intermediate cylinder 12 injects nutrient solution is 80 ~ 90L.
Preferably, the liquid measure that in described step (5), each intermediate cylinder 12 injects nutrient solution is 80 ~ 90L
That injects in above-mentioned steps (2) and step (5) to dwell the nutrient solution of micro-algae for cultivating the end, and the concentration range of each component is respectively: NaNO
3: 17.0 ~ 13.0g/L, K
2hPO
4: 4.0 ~ 1.0g/L, MgSO
47H
2o:4.0 ~ 2.0g/L, CaC
l22H
2o:2.0 ~ 1.0g/L, citric acid: 0.5 ~ 0.1g/L, ferric ammonium citrate: 0.5 ~ 0.1g/L, EDTANa
2: 0.1 ~ 0.3g/L, Na
2cO
3: 2.0 ~ 0.5g/L, H
3bO
3: 3.5 ~ 1.5g/L, MnC
l24H
2o:3.0 ~ 1.0g/L, ZnSO
47H
2o:0.8 ~ 0.1g/L, Na
2moO
42H
2o:0.8 ~ 0.1g/L, CuSO
45H
2o:0.2 ~ 0.05g/L, Co (NO
3)
26H
2o:0.1 ~ 0.02g/L.
The above is only preferred implementation of the present utility model; it should be noted that; for those skilled in the art; under the prerequisite not departing from the utility model principle; can also make some improvements and modifications, these improvements and modifications also should be considered as protection domain of the present utility model.
Claims (4)
1. can realize the photo-biological reactor that continuity is cultivated, it is characterized in that comprising reactor and liquid in-out pipeline; Described reactor comprises the intermediate cylinder of at least one hollow structure, and the top bolt of described intermediate cylinder is connected with top cover, and its bottom end seal has bottom; Described top cover has top cover venting hole and an inlet opening; Described liquid in-out pipeline comprises feed liquor main road, and described feed liquor main road is connected with the quantity feed liquor branch road consistent with described intermediate cylinder quantity, and described feed liquor branch road is connected with the inlet opening on described top cover, described feed liquor branch road is provided with feed liquor branch road valve; The circle centre position of described bottom is provided with a fluid hole, and described fluid hole is connected with fluid branch road; Described fluid branch road is connected with fluid main road, and described fluid main road is connected to algae liquid holding tank; Described bottom is provided with multiple air inlet port at the periphery of described fluid hole; Admission passage is connected with below described air inlet port; The arranged outside of described intermediate cylinder has LED lamp tube, and described LED lamp tube is fixedly connected with by the outer wall of support with described intermediate cylinder; Described LED lamp tube is connected with LED power adjustable switch; The inside of described intermediate cylinder is provided with pH detecting electrode and temperature sensor.
2. a kind of photo-biological reactor realizing continuity and cultivate according to claim 1, is characterized in that described bottom is provided with bend pipe of giving vent to anger on described air inlet port, described in bend pipe of giving vent to anger be communicated with described admission passage.
3. a kind of photo-biological reactor realizing continuity and cultivate according to claim 1, bend pipe of giving vent to anger described in it is characterized in that is made up of vertical portion and bend, and the angle that vertical portion and bend are formed is 45 °.
4. a kind of photo-biological reactor realizing continuity and cultivate according to claim 1, is characterized in that the angle that the wire of the projection of described bend on described bottom and described vertical portion and described fluid hole is formed is 45 °.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420435048.0U CN204298406U (en) | 2014-07-31 | 2014-07-31 | A kind of photo-biological reactor realizing continuity and cultivate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420435048.0U CN204298406U (en) | 2014-07-31 | 2014-07-31 | A kind of photo-biological reactor realizing continuity and cultivate |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204298406U true CN204298406U (en) | 2015-04-29 |
Family
ID=53103963
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420435048.0U Active CN204298406U (en) | 2014-07-31 | 2014-07-31 | A kind of photo-biological reactor realizing continuity and cultivate |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN204298406U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104152344A (en) * | 2014-07-31 | 2014-11-19 | 天津爱微生物科技有限公司 | Photobioreactor used for culturing benthic microalgae and culturing method thereof |
-
2014
- 2014-07-31 CN CN201420435048.0U patent/CN204298406U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104152344A (en) * | 2014-07-31 | 2014-11-19 | 天津爱微生物科技有限公司 | Photobioreactor used for culturing benthic microalgae and culturing method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104152344A (en) | Photobioreactor used for culturing benthic microalgae and culturing method thereof | |
| CN103583350B (en) | Indoor round-the-clock full-automatic no-soil cultivation incubator | |
| CN109136082A (en) | A kind of membrane bioreactor and helotism system of microalgae and methane-oxidizing bacteria symbiosis reinforcing methane producing bio-oil | |
| CN103865793A (en) | Nostoc alga superficial layer culture system and culture method | |
| CN107189930A (en) | Indoor microalgae culture system and its cultural method | |
| CN104521726B (en) | Grading breeding device for breeding through biogas slurry and breeding method of grading breeding device | |
| CN104770304B (en) | Plant bioreactor and its application method | |
| CN204022814U (en) | A kind of full-automatic algatron | |
| CN102533522B (en) | Full-plastic airtight modularized airlift light biological reactor | |
| CN201729830U (en) | Sealed pipeline culturing device for bait microalgae | |
| CN202415535U (en) | Scale microalgae cultivation bioreactor | |
| CN203462044U (en) | Cylindrical photobioreactor for bait microalgae cultivation | |
| CN101906380B (en) | Seal type pipeline culture device of bait microalgae and microalgae pipeline culture method | |
| CN204298406U (en) | A kind of photo-biological reactor realizing continuity and cultivate | |
| CN105838585A (en) | Device and method for cultivating diatom | |
| CN109456885A (en) | A kind of automatically cleaning fluidized bed bioreactor | |
| CN104480178A (en) | Method for rapidly accumulating astaxanthin by forcing haematococcus pluvialis | |
| CN205774490U (en) | Diatom culture apparatus | |
| CN207355081U (en) | A kind of culturing frame for being used to prepare selenium-rich agaric | |
| CN206751785U (en) | A kind of diatom cultivates plate-type reactor | |
| CN206220589U (en) | Fish and vegetable symbiotic Intelligent culture system | |
| CN106244426A (en) | High density Large-scale microalgae culture apparatus | |
| CN106635768B (en) | Biological microalgae photosynthetic reactor and its application method | |
| CN206706102U (en) | A kind of novel photo-biological reactor | |
| CN102358885A (en) | Production method of cylindrotheca clostetium |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |