CN105316217B - Artificial light source both culturing microalgae equipment - Google Patents
Artificial light source both culturing microalgae equipment Download PDFInfo
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- CN105316217B CN105316217B CN201510189268.9A CN201510189268A CN105316217B CN 105316217 B CN105316217 B CN 105316217B CN 201510189268 A CN201510189268 A CN 201510189268A CN 105316217 B CN105316217 B CN 105316217B
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- light source
- culturing microalgae
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/04—Apparatus for enzymology or microbiology with gas introduction means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/36—Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/36—Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
- C12M1/38—Temperature-responsive control
Abstract
The invention discloses a kind of artificial light source both culturing microalgae equipments, comprising: reaction vessel, reaction vessel is equipped with cover board and reaction vessel accommodates the culture solution for micro algae growth；Charging/discharging device, microalgae are pumped into or are pumped out reaction vessel by charging/discharging device；Light emitting device, for light source needed for generating micro algae growth；Nutrition distribution device, nutrition needed for nutrition distribution device is used into reaction vessel provide micro algae growth；And gas distributor, gas needed for gas distributor is used into reaction vessel provide micro algae growth.It is also provided with infrared light supply in cultivation equipment of the invention, suitable manganese ion is provided in culture solution and is passed through suitable carbon dioxide, so that both culturing microalgae efficiency greatly promotes.And artificial light source both culturing microalgae equipment controllability of the invention is stronger, is advantageously implemented steady and sustained the factorial production.
Description
Technical field
The present invention relates to field of biotechnology, and in particular to artificial light source both culturing microalgae equipment.
Background technique
Microalgae biomass is the future thrust of biomass economy, has the characteristics that energy conversion efficiency is high, and common
Terrestrial crops are compared, and the yield of unit area can be higher by decades of times, so that industrial aquaculture can be realized.Micro algae growth it is basic
Principle is using microalgae photosynthesis, is the biomass of microalgae itself to secure carbon by carbon dioxide conversion, and lead to
Cross the biomass such as characteristic and function Synthetic Oil, protein, polysaccharide, the cellulose of microalgae itself.Using either physically or chemically
Utility in microalgae cell is transformed into extracellularly, then carries out refinement processing, to produce biodiesel, algal polysaccharides
Equal products.It as a result, in actual production, can be by the photosynthesis of algae, by the nutriment and dioxy in waste water and gas
Change carbon conversion is the product that bio-fuel, protein etc. have high utility value.It is substantially increased in oil price, food shortage problem
Outstanding day by day today, the industry have vast potential for future development.
To further increase the specific yield of microalgae biodiesel, realizing three dimensional-breeding, it is necessary to use dedicated microalgae
Cultivate Photoreactor.Although culturing microalgae photoreactor in the prior art compares raceway pond, multistage pond etc. are outdoor directly to support
It grows, cultivation density, yield per unit area are all significantly improved, but since micro algae growth and effective substance directional induction produce when institute
Light source is still natural light, and microalgae has uncertain and uncontrollability, with factory's metaplasia to the utilization of sunlight and insufficient
It produces stable, lasting requirement to run in the opposite direction completely, the auxiliary breeding function such as nutritional supplementation, carbon dioxide inflation is also not implemented in addition
Automation, makes it still have relatively large distance with practical industrial application.
Therefore, there is an urgent need in the art to a kind of technologies can overcome above-mentioned both culturing microalgae light reaction in the prior art at present
The drawbacks of poor controllability, the degree of automation of device be low, low efficiency.
Summary of the invention
The object of the present invention is to provide one kind can be optimal according to the cultivation demand of variety classes algae different phase proportion
The artificial light source both culturing microalgae of the elements such as light source, carbon dioxide, nutrition, temperature, flow velocity is equipped, to realize ultra high density microalgae
It is the stabilization of cultivation, controllable, meet industrialization demand.
In order to achieve the above objectives, the present invention provides a kind of artificial light source both culturing microalgae equipment, the artificial light source microalgaes
Cultivation is equipped
Reaction vessel, the reaction vessel is equipped with cover board and the reaction vessel accommodates the culture solution for micro algae growth；
Charging/discharging device, the charging/discharging device and the reaction vessel are tightly connected；
Light emitting device, the light emitting device are located at reaction vessel interior and when reactor work, the luminous dress
It sets and is at least partially or fully immersed in the culture solution, thus light needed for providing micro algae growth in reaction vessel, wherein
The light intensity for the light that the light emitting device is issued is uniform or generally uniform in the depth direction (Z-direction) of reaction vessel
's；
Nutrition distribution device, the nutrition distribution device can provide manganese ion into the culture solution；With
Feeder, gas needed for the feeder is used into the reaction vessel provide micro algae growth.
In a preferred embodiment, the manganese ion source in the culture solution comes from MnCl2·4H2O, and every liter of culture
The MnCl added in liquid2·4H2The quality of O is 5-50mg.
In a preferred embodiment, the light that at least three different wave lengths are emitted when the light emitting device works, including
Light and wavelength that light that wavelength is 600-680nm, wavelength are 420-500nm are the light of 700-780nm；And/or
The colour temperature for the complex light that the luminescence unit issues is 1000-20000K, preferably 1500-6000K.
In a preferred embodiment, the light of three different wave lengths include wavelength be 650-670nm light, wavelength be
The light and wavelength of 440-460nm is the light of 710-730nm.
In a preferred embodiment, the light emitting device includes light guide plate and luminescence unit, and the luminescence unit is embedded in institute
Light guide plate is stated, the light that the luminescence unit generates makes light guide plate Integral luminous through light guide plate conduction, and the light emitting device also wraps
Bracket is included, the bracket supports the light guide plate for fixed, and the bracket and the reaction vessel and/or the cover board are removable
It connects with unloading；And/or
In the light emitting device, luminescence unit LED, and the quantity of LED is 1-10000/ light guide plate；Preferably 10-
1000/ light guide plate.
In a preferred embodiment, the feeder can be passed through the mixing of carbon dioxide and air into the culture solution
Object, wherein carbon dioxide accounts for the 0.1-10% of mixed gas total volume, preferably accounts for 0.5-5%；And/or
It is 0.1-1MPa that carbon dioxide, which is passed through pressure,；And/or
It is 0.1-50nm that carbon dioxide, which releases aperture,.
In a preferred embodiment, it is additionally provided in the cultivation equipment for detecting and controlling titanium dioxide in the culture solution
The monitoring of carbon intake and automatic control system, wherein
It is carbonated when the monitoring and automatic control system detect the pH >=5.5 in the culture solution
Mixed gas is continually fed into the culture solution；And suspend as the PH in the culture solution < 5.5, in the culture solution logical
Enter carbon dioxide.
In a preferred embodiment, temperature control device is equipped with around the reaction vessel, the temperature control device is for maintaining
Liquid environment temperature in reaction vessel, which is in, to be suitble in the range of micro algae growth；And/or
The feeder includes that gas refinement distributor has one during being passed through gas into reaction vessel
The gas of constant-pressure is uniformly escaped carefully scatteredly by the aperture of the refining device, so that gas and nutriment be promoted to be scattered in liquid
In cultivating system.
In a preferred embodiment, it is additionally provided in the artificial light source both culturing microalgae equipment for monitoring in the culture solution
The water quality monitor of manganese ion or other ion concentrations, the intake of the water quality monitor are located at liquid level hereinafter, wherein
When the water quality monitor monitors the concentration of manganese ion or other ions in the culture solution lower than present count
When value, the water quality monitor can issue prompting, so that the nutrition distribution device carries out adding for manganese salt or other nutritive salt
Add.
In a preferred embodiment, the artificial light source both culturing microalgae equipment is suitable for chlorella, scenedesmus, haematococcus pluvialis
The cultivation of equal fresh water eukaryotic algae.
In a preferred embodiment, the inoculum density of the algae cultivated is 0.1g/L-2g/L (algae dry weight/culture solution
Volume), more preferably 0.2g/L-0.6g/L.
In a preferred example, horizontal direction (including X-axis and Y of the light intensity for the light that light emitting device is issued in reaction vessel
Axis direction) it is substantially uniform.
In a preferred example, described " uniform or substantially uniform " the light intensity D1 for referring to any depth in entire depth
The average intensity Dm of range meets following formula:
1.5≥D1/Dm≥0.7。
Preferably, 1.2 >=D1/Dm >=0.8；More preferably 1.1 >=D1/Dm >=0.9.
In a preferred example, temperature control device is equipped with around reaction vessel, the temperature control device is for maintaining in reaction vessel
Liquid environment temperature be in be suitble to micro algae growth in the range of.
In a preferred example, the cultivation temperature in the cultivation equipment is 10-30 DEG C, is more preferably 20-30 DEG C.
In a preferred example, which may include electric heating or steam-heating system, cold water cooling system etc., such as
Temperature control water pipe.
In a preferred example, artificial light source both culturing microalgae equipment is additionally provided with monitoring system, for monitoring the ginseng of liquid environment
Number, parameter are selected from: pH value, temperature and/or nutriment concentration.
In a preferred example, it is equipped with venthole on the cover board.
In a preferred example, it is equipped with muti-piece light guide plate in artificial light source both culturing microalgae equipment, preferably 30-1000 block,
More preferably 40-800 block, most preferably 50-500 block.
In a preferred example, light guide plate is made of the transparent organic material with anti-weak acid performance.
Detailed description of the invention
Fig. 1 is the three-dimensional cutaway view of artificial light source both culturing microalgae equipment according to an embodiment of the invention；
Fig. 2 is the perspective cross-sectional schematic diagram of artificial light source both culturing microalgae equipment according to an embodiment of the invention；
Fig. 3 is the main view schematic cross-sectional view of artificial light source both culturing microalgae equipment according to an embodiment of the invention；
Fig. 4 is the vertical view schematic cross-sectional view of artificial light source both culturing microalgae equipment according to an embodiment of the invention；
Fig. 5 is the three-dimensional signal of the light emitting device of artificial light source both culturing microalgae equipment according to an embodiment of the invention
Figure；
Fig. 6 a is the main view of the light emitting device of artificial light source both culturing microalgae equipment according to an embodiment of the invention；
Fig. 6 b is the side view of the light emitting device of artificial light source both culturing microalgae equipment according to an embodiment of the invention；
Fig. 6 c is the top view of the light emitting device of artificial light source both culturing microalgae equipment according to an embodiment of the invention；
Fig. 6 d is the enlarged drawing according to part B in Fig. 6 b；
Fig. 7a-7c is the simplification schematic top plan view of the artificial equipment for microalgae cultivation of other embodiments according to the present invention；And
Fig. 8 is the representative side section view of the artificial equipment for microalgae cultivation of other embodiments according to the present invention.
Specific embodiment
The present inventor after extensive and in-depth study, develops a kind of artificial light source both culturing microalgae equipment for the first time, passes through
The structures such as the light emitting device of special designing, reactor of the invention not only shortens incubation time, but also can significantly improve microalgae
Biomass density and total substance content, so as to prepare the better biomass material of quality and product.It is complete on this basis
At the present invention.
Presently preferred embodiments of the present invention is described in detail below with reference to attached drawing, it is of the invention to be clearer to understand
Objects, features and advantages.It should be understood that embodiment shown in the drawings does not limit the scope of the present invention, and only it is
Illustrate the connotation of technical solution of the present invention.
Bioreactor
It is raw that built-in light source bioreactor artificial light source both culturing microalgae equipment of the invention is suitable for chlorella, scenedesmus, rain
The high density industrialization of the fresh water eukaryotic algae such as haematococcus cultivates.
As depicted in figs. 1 and 2, the artificial light source both culturing microalgae equipment includes reaction vessel 1, light emitting device 2, input and output material
Device 3, cover board 5, gas distributor 6, nutrition distribution device, temperature control device 7 and monitoring and control system etc..Reaction vessel 1
It is equipped with cover board 5, reaction vessel 1 and charging/discharging device 3 are tightly connected, and charging/discharging device is equipped with valve and switch, is used for
The on or off of input and output material is controlled, it is steady needed for micro algae growth each stage for generating to be equipped with light emitting device 2 in reaction vessel 1
Determine light source, the bottom of reaction vessel 1 is equipped with gas distributor 6 for gas needed for being passed through micro algae growth each stage, nutrition cloth
Material device is equipped with temperature control device 7 (being illustrated as temperature control water pipe) for supplying nutrients into reaction vessel, around reaction vessel and uses
In keeping the temperature of solution in reaction vessel to breed in suitable microalgae quantity and within the temperature range of individual growth.
The lower part (such as from about 30%~90% or 50%~70% height) of the bioreactor can be embedded in underground.
The bioreactor may be provided at around power plant, and the waste water and gas generated using power generation is as the object of both culturing microalgae
Matter and energy source, have the function of environmental emission reduction.The reaction vessel 1 can be lighttight closed container, rainy days with
Night can also be carried out by the light emitting device of itself it is luminous, for used in micro algae growth.The cultivation equipment in be equipped with monitoring and
Automatic control system, for monitoring water temperature, pH value, CO2 concentration, TDS, absorbance, luminous intensity etc., and can be right as needed
Above-mentioned condition carries out automation control.
Fig. 3 is the main view schematic cross-sectional view of artificial light source both culturing microalgae equipment；As shown in figure 3, in the wall of reaction vessel
It is equipped with temperature control device 7, temperature control device 7 can be any suitable form, including electric heating or steam-heating system, cold water drop
Warm system, such as temperature control spacer etc..Preferably, temperature control device 7 is temperature control water pipe, the giving up with certain temperature of power plant discharge
Water can be used in circulation in temperature control water pipe to keep the temperature in bioreactor between 20 DEG C~30 DEG C.
It is equipped with venthole at the top of the cover board 5 of bioreactor, for discharging the oxygen of microalgae photosynthesis generation.
Fig. 4 is the vertical view schematic cross-sectional view of artificial light source both culturing microalgae equipment.As shown, being supported in artificial light source microalgae
The bottom for growing equipment is equipped with gas distributor 6, and gas distributor 6 can be rotary, slot type, tubular type or other any suitable shapes
Formula.In microalga cultivation process, carbon dioxide, air or other gases are passed through into reaction vessel by gas distributor.Compared with
Goodly, rotary gas distributor is used in this example, during being passed through gas, the rotation of gas distributor 6, which drives, to be passed through
Gas and bottom container in liquid disturbance, be uniformly distributed in the solution to be conducive to gas with nutriment.
In one or more positions of reaction vessel (such as on chamber wall, on the inside of container bottom, container top and cover board
Deng) installation nutrition distribution device.Nutrition distribution device provides different nutriment in the different phase of micro algae growth.
Other nutriments is passed through rate and CO in reaction vessel2Be passed through rate and can pass through CO at certain ratio2
On-line monitoring system can determine CO in solution2Meltage, while by monitor system monitoring solution pH value know solution
PH value.The CO that control system can control2With the rate that is passed through of nutriment, guarantee the meltage of nutriment in solution
And pH value is bred in suitable micro algae growth or in the range of oil-producing.
Reaction vessel of the invention can be used glass reinforced plastic and be made, and reaction vessel can have any suitable shape and
Size.Vessel surface is more smooth, and preferably, vessel surface has moveable closed cover board.
As shown in Fig. 7a-7c, which can be long annular stereo container.The total length of the container is 2m-500m,
Preferably 10m-200m；Container section depth-width ratio is 0.5:1-1:0.5；Height is 20cm-400m, is preferably 50cm-
200cm.Preferably, minimum point is located at center, both sides bottom gradient as shown in figure 8, low both sides are high among the bottom section of container
It is 5 ° -60 °, is more preferably 10 ° -30 °.
Section-variable of the container in middle section turns to closed cylinder, drum diameter 20cm-400m, more preferably for
50cm-200cm, the partial-length can be 20cm-100cm, more preferably can be 20cm-50cm.The different cross section shape of container
Conversion portion is in be smoothly connected.
Cultivation equipment of the invention further includes other feeders, such as gas source not shown in the figure, mixer, appendix
Deng.Gas source is mainly air and carbon dioxide in the present invention.Mixer is mainly used for mixing air and carbon dioxide, and mixer can
Using Venturi tube or static mixer.Blowing mouth material can be ceramic capillary or resin microcellular structure, micro-pore diameter 0.1-
10 microns, be more preferably 0.5-5 microns.Gas distributor is set to Cultivation container bottom, and the lower end distance of gas distributor is held
Bottom 0-5cm in device.As shown, reaction container bottom can be equipped with multiple gas distributors being equidistantly spaced from side by side.In use,
The supply gas pressure of gas distributor is 0.2-20Mpa, is more preferably 0.5-5Mpa.
Preferably, the nutrition distribution device in cultivation equipment of the invention can be for positioned at the perforated tube of upper vessel portion, battalion
Nutrient solution can be uniformly sprayed in the culture solution in container after pipeline is flow to above container through aperture.In addition, inventor is by real
It tests and probes into, discovery provides a certain amount of manganese ion in culture solution, can effectively improve culture efficiency.Therefore, of the invention
The nutrition distribution device of cultivation equipment manganese ion can be provided into culture solution.
Preferably, cultivation equipment of the invention is additionally provided with circulation propulsion device, circulation propulsion device can have propeller type
Or the propulsion blade of screw, flowing and circulation for water body in system.Propulsion blade revolving speed is 10-1000rpm, more preferably
For 20-400rpm.
In addition, cultivation equipment of the invention can also integrate the prison of the online water quality for detecting manganese ion concentration in culture solution
Instrument is surveyed, so that the manganese ion concentration controlled in culture solution reaches the range most beneficial for both culturing microalgae.The water of the water quality monitor
Sample intake is located at liquid level hereinafter, can periodically sample automatically.Pass through microfiltration system, mistake before water sample is automatically fed into detector
Filtering precision is T grades.When monitoring manganese ion content lower than 5mg/L (or numerical value of other settings), system automatically turns on nutrition
Salt addition reminds administrative staff to carry out nutritive salt feed supplement.
The pH range of culture solution in cultivation equipment of the invention is 5-10, is more preferably 6-8.Cultivation equipment of the invention
PH on-line monitoring sensor can also be integrated and be passed through carbon dioxide in culture solution for measuring the pH value in culture solution to control
Concentration.When pH is higher than 6.5 (or numerical value of other settings) in culture solution, system automatically turns on carbon dioxide valve, Xiang Chi
In be passed through carbon dioxide gas；When ph is lower than 6.5 (or numerical value of other settings), system closes carbon dioxide valve, ventilation
Device Xiang Chizhong is passed through air or directly stops being passed through gas, thus the intake of effectively regulation of carbon dioxide, to obtain
The best carbon dioxide conditions of micro-algae propagation and growth.
Light emitting device
Fig. 5 is the stereoscopic schematic diagram of light emitting device 2.The setting of light emitting device so that bioreactor can without sunlight or its
The normal operations such as breeding production are carried out in the case where his external light source.As described in Figure 5, the light emitting device is sent out by bracket 9, LED
Light unit group 10 and light guide plate 11 are constituted, and light guide plate 11 is fixed by bracket 9, and the top of bracket 9 is fixed on cover board 5, and LED shines
Unit group 10 is located at the top of light guide plate 11, and light guide plate 11 or whole protrude under liquid level.In bioreactor of the invention,
Equipped with multiple light emitting devices 2, each light guide plate can be installed individually or dismantle.
Light guide plate can be made of transparent organic material (such as acrylic), and have anti-weak acid performance.LED luminescence unit group
It is directly contacted at the top of light guide plate with light guide plate, the light that LED luminescence unit group issues can pass through light guide plate and conduct and make leaded light
Plate Integral luminous.Preferably, light guide plate is placed in parallel, the distance between two boards be can be that 5cm-50cm is more preferably
10cm-20cm.Luminescent screen illumination, which can according to need, to be adjusted.
The top of the bracket 9 of light emitting device 2 and cover board 5 are detachably connected, consequently facilitating the dismounting of light emitting device, replacement
Or it reinstalls；The suspension of light emitting device 2 is in the reaction vessel without the bottom of contact container, because without influencing container bottom gas
The rotation of distributor.It should be understood that the bracket of light emitting device can also be in any other suitable manner in any suitable position
It is fixed with reaction vessel.
Fig. 6 a-6c is main view, side view and the top view of the light emitting device of artificial light source both culturing microalgae equipment respectively；Figure
6d is the enlarged drawing of part B in Fig. 6 b.
LED luminescence unit group 10 includes LED luminescence unit small one by one, and light caused by luminescence unit can be compound
Color or monochromatic light, monochromatic LED emission wavelength range are 350-900nm, and complex light reference color temperature is 1500-20000K.Individually
The quantity of LED luminescence unit on light guide plate can be 1-10000.
Light source is LED light source
Light emitting device can emit the light of different wave length when working.The light of the different wave length includes: wavelength are as follows: 600-
The light A of 680nm, more preferably 650-670nm；Wavelength is 420-500nm, the light B of more preferably 440-460nm；700-780nm, more
The light C (infrared light) of good ground 710-730nm.
Inventor pass through Experimental Research deep for a long time, find the issued different wave length of light emitting device light proportion with
When lower range, the both culturing microalgae benefit in cultivation equipment can reach higher level.Inventor is found through experiments that, same illumination
In the case where power, after the near infrared light for supplementing specific wavelength, microalgae output increased 10-25% can be made:
A:B=10:1~2:1；
A:C=20:1~3:1.
In addition, the intensity of illumination of light emitting device is adjustable, when illumination bright dark alternating (luminous power variation).Preferably,
The 0%-80% of luminous power is more preferably 0%-60% when luminous power is light when half-light.Preferably, half-light irradiation time
For the 10%-300% more preferably 20%-100% of bright light exposure time
The light intensity for the light that wherein light emitting device is issued is uniform or big in the depth direction (Z-direction) of reaction vessel
It causes uniform；The light intensity for the light that light emitting device is issued is substantially equal in the horizontal direction (including X-axis and Y direction) of reaction vessel
It is even." uniform or substantially uniform " refers to the light intensity D1 of any depth and in the case where the average intensity Dm of entire depth range meets
Formula:
1.5≥D1/Dm≥0.7。
Preferably, 1.2 >=D1/Dm >=0.8；More preferably 1.1 >=D1/Dm >=0.9.
In fact, LED luminescence unit group 10 can be located at light guide plate 11 bottom or embedded in luminescent screen other are suitable
Position, as long as guaranteeing that the light that it is issued can conduct in monolith light guide plate so that monolith light guide plate is luminous.
Preferably, the electric energy that the energy source of LED luminescence unit group generates for solar energy tabula rasa absorption solar energy.So
Since, though bioreactor of the invention does not utilize solar energy directly, pass through solar energy tabula rasa for unstable solar energy
It collects for generating electricity, the electricity generated is steadily supplied to the luminescence unit group of bioreactor to send out it constantly
Light guarantees stabilization, the duration of both culturing microalgae.
Produce cultivating system
The quantity breeding of microalgae and individual weight gain stage can carry out in the same bioreactor, but need with growth
Progress, replace light emitting device, change the nutriment type and rate that are passed through, process is complex.
In order to realize efficient large-scale farming, multiple bioreactors can be used and be connected in series or in parallel large-scale life
Produce cultivating system.Serial or parallel connection between bioreactor is connected by feed inlet and outlet, can complete biology by pumping system
Charging and discharging between reactor.
In view of the quantity variation in microalga cultivation process, the demand of light source and nutriment is also become constantly
Change, the concatenated cultivating system of multiple bioreactors can be used, for example, being completed using the concatenated system of three bioreactors micro-
Whole growth courses of algae production:
Suitable algae is put into first order bioreactor, breeding increase to certain amount, by feed inlet and outlet into
Enter second level bioreactor and carry out further growth breeding, then enters back into third level bioreactor, no longer breed, it is only main
Carry out individual growth, and less breeding.
Emission wavelength is provided in the first order and second level bioreactor to be suitable only for being conducive to shining for micro-algae propagation
Device, and nutrition distribution device is passed through the nitrogen oxides such as ammonium phosphate, potassium dihydrogen phosphate or dipotassium hydrogen phosphate work into reaction vessel
For nitrogen phosphorus source, while nutritive salt necessary to other micro algae growths such as add iron, zinc, gas distributor are passed through into reaction vessel
Nitrogen oxides and CO2, luminous intensity and nutriment delivery rate are compared with first order bioreactor in the bioreactor of the second level
Greatly, to adapt to the growth demand of microalgae that breeding increases；Emission wavelength is provided in third level bioreactor to be suitable only for
Conducive to the light emitting device of microalgae individual growth, PH and temperature etc. are adjusted to the condition of suitable microalgae individual growth, gas point simultaneously
Cloth device is passed through CO2 into reaction vessel.
Bioreactor not at the same level can select different sizes, such as the size of first order bioreactor according to demand
Two-stage is smaller after relatively.
It, can be to first order bioreactor after microalgae in first order bioreactor is expelled to second level bioreactor
It is passed through new algae, while carrying out new round cultivation.In order to improve the purity of algae, can be discharged from second level bioreactor
Algae of the part as first order bioreactor is filtered out in microalgae.
Cultural method
The present invention provides a kind of methods for cultivating microalgae, and the method comprising the steps of:
1. providing artificial light source both culturing microalgae equipment of the invention；
2. algae is invested in artificial light source both culturing microalgae equipment, provides into reactor and supported needed for microalgae existence
Material (including nitrogen source, phosphorus source, inorganic salts (such as manganese, iron, zinc, magnesium etc.) and carbon dioxide or air are provided, it opens light emitting device and produces
Light needed for raw micro algae growth.
It is suitable for the invention microorganism to be not particularly limited, as long as it is grown using light source.It is representative micro-
Biology includes (but being not limited to): Chlorophyta, Cyanophyta, Chrysophyta and rhodophyta microalgae.A kind of preferred microorganism is bead
Algae (Chlorellales) fresh water species.It is raw red that cultivation equipment and breeding technique of the invention is particularly suitable for chlorella, scenedesmus, rain
The cultivation of the fresh water eukaryotic algae such as ball algae.
Nutritional ingredient
The inoculum density of institute's cultivating microalgae is that (microalgae is dry by 0.1g/L-2g/L in the reaction vessel of cultivation equipment of the invention
Weight/nutrient solution volume), more preferably 0.2g/L-0.6g/L.Nutritional ingredient provided by nutrition distribution device and feeder
Supply should be determined according to being inoculated with and raising concentration, should properly increase nutrient concentration when being inoculated with and raising concentration improves,
And the consumption of holding and extra-nutrition salt in the breeding process.
Cultivation equipment of the invention can be passed through carbon dioxide as carbon source.It is passed through preferably, carbon dioxide mixes with air,
Wherein carbon dioxide accounts for the 0.1-10% of mixed gas total volume, preferably 0.5-5%；It is 0.1- that carbon dioxide, which is passed through pressure,
1MPa；It is 0.1-50nm more preferably 1-20nm that carbon dioxide, which releases aperture,.
The pH range of culture solution in cultivation equipment of the invention is 5-10, is more preferably 6-8.In order to preferably control training
Carbon dioxide is passed through two in nutrient solution, in a preferred embodiment, as the pH in culture solution >=5.5, may be flowed into containing two
The mixed gas of carbonoxide；And as PH < 5.5, ventilation can be suspended.
The one or more of following nitrogenous compound may be selected in nitrogen source in culture solution, and nitrogenous in various every liter of culture solution
The intake of compound approximately as:
CO(NH2)2, 500-1500mg/L；
NaNO3, 1000-3000mg/L；
NH4HCO3, 1000-3000mg/L；
NH4NO3, 500-1500mg/L.
The one or more of following phosphorus-containing compound may be selected in phosphorus source in culture solution, and phosphorous in various every liter of culture solution
The intake of compound approximately as:
K2HPO4, 20-80mg/L；
KH2PO4, 20-80mg/L.
Inventor's in-depth study by long-term, when suitable manganese ion is added in discovery in culture solution, breeding efficiency
It greatly promotes.For example, the MnCl2 that concentration is 5-50mg/L (weight of additive/nutrient solution volume) can be added in culture solution
4H2O.Preferably, usually the culture medium BG11 Medium Proportion that uses of algae at cultivating system in MnCl24H2O concentration be
1.86mg/L。
Other than above-mentioned carbon source, nitrogen source, phosphorus source, manganese ion, other following nutritive salt, and its are usually added in culture solution
His concentration range of nutritive salt is as follows:
MgSO4·7H2O, 70-150mg/L；
CaCl2·2H2O, 30-80mg/L；
Lemon, 5-10mg/L；
Ironic citrate, 5-50mg/L；
H3BO3, 1.5-5mg/L；
ZnSO4·7H2O, 0.2-0.5mg/L；
Na2MoO4·2H2O, 0.2-0.8mg/L；
CuSO4·5H2O, 0.05-0.3mg/L；
Co(NO3)2·6H2O 0.03-0.2mg/L。
Preparation method
The present invention provides a kind of method for preparing microalgae biomass, this method uses the microalgae prepared by preceding method
Algae solution is processed as raw material, thus be made microalgae finished product, a kind of typical process comprising steps of
Microalgae is collected using the method that filtering, filters pressing or bubble suspend, low temperature drying can be obtained micro- after then crushing
Algae finished product.
Embodiment 1
Micro-algae propagation is carried out in microlamp's experimental box, selected algae is chlorella pyrenoidosa, and inoculum density is
0.3g/L (dry weight).Artificial light source is three-color LED light source, and wavelength is respectively the color of 650 ± 5nm, 440 ± 5nm, 720 ± 10nm, three
Light source proportion is 10:3:2, and light most dark place light intensity is about 3000lx, and bright dark alt time is 10 hours (bright): 2 hours (dark),
Half-light power is about the 40% of light power.It is passed through carbon dioxide, pressure 0.4Mpa, the aperture for the ceramic membrane pressure tube released
For 10nm, it is 2%-3% that carbon dioxide, which mixes with air accounting, and Continuous aeration to PH=6.5 or so stops, and reduces carbon dioxide
Accounting is mixed with air, until the section PH remains between 6.5-6.8.Cultivation temperature remains 27 degree ± 1 degree.
The nutrition selection of culture solution in the present embodiment are as follows:
CO(NH2)2, 800mg/L；
NaNO3, 1200mg/L；
KH2PO4, 50mg/L；
MgSO4·7H2O, 100mg/L；
CaCl2·2H2O, 40mg/L；
Citric acid, 6mg/L；
Ferric citrate, 25mg/L；
H3BO3, 3mg/L；
MnCl2·4H2O, 12mg/L；
ZnSO4·7H2O, 0.3mg/L；
Na2MoO4·2H2O, 0.4mg/L；
CuSO4·5H2O, 0.1mg/L；
Co(NO3)2·6H2O, 0.06mg/L.
Daily monitoring ion concentration, and appropriate feed supplement holding nutrition more than three times.
It after cultivation 7 days, collects, filter, weigh after drying, obtaining final biomass concentration is 1.07g/L.
Comparative example 1:
Using same method, but MnCl2·4H2O concentration becomes 1.8mg/L, after cultivation 7 days, collects, filters, claims after drying
Weight, obtaining final biomass concentration is 0.71g/L.
Comparative example 2:
Using same method, but MnCl24H2O is not added in culture medium, after cultivation 7 days, collect, filter, claim after drying
Weight, obtaining final biomass concentration is 0.42g/L, and yellowish, appearance float dead algae.
Comparative example 3:
Using same method, but MnCl24H2O concentration becomes 100mg/L, after cultivation 7 days, collects, filters, after drying
Weighing, obtaining final biomass concentration is 0.52g/L, occurs floating dead algae.
Comparative example 4:
Using same method, but artificial light source is dichromatism LED light source, and wavelength is respectively 650 ± 5nm, 440 ± 5nm, dichromatism
Light source proportion is 10:3, after cultivation 7 days, collects, filters, weighs after drying, obtaining final biomass concentration is 0.82g/L.
Comparative example 5:
Using same method, but it is not passed through carbon dioxide, is only passed through air, after cultivation 7 days, collected, filters, claims after drying
Weight, obtaining final biomass concentration is 0.79g/L.
Comparative example 6:
Using same method, but obstructed any gas, frond settle, and can not be cultivated.
Conclusion
By the experimental result of above embodiment and comparative example it can be seen that
1. MnCl in embodiment 12·4H2The concentration of O is 12mg/L, and the final biomass concentration that cultivation obtains after 7 days is
1.07g/L, and in comparative example 1-3 or reduce or increase MnCl2·4H2The concentration of O, discovery cultivation obtain final after 7 days
Biomass concentration is all reduced compared with embodiment 1, and occurs the dead algae of floating in some comparative examples, it can thus be seen that training
The growth that suitable manganese ion is conducive to cultivated algae in cultivation equipment really is added in nutrient solution；
2. use proportion for the three-color LED light source of 10:3:2 in embodiment 1, and it is infrared using not emitting in comparative example 4
The dichromatism LED light source of light, other condition of culture under the same conditions, the cultivation achievement of embodiment 1 is substantially better than comparative example 4,
It can thus be seen that a certain proportion of infrared light supply is conducive to the growth of cultivated algae in cultivation equipment；
3. the carbon dioxide of certain mixing accounting and the mixed gas of air are passed through in embodiment 1, and it is obstructed in comparative example 5
Enter carbon dioxide, be not passed through any gas in comparative example 6, can be seen that the dioxy for being passed through appropriate mixed proportion from cultivation achievement
Change carbon and air, the growth and breeding to algae are very necessary.
All references mentioned in the present invention is incorporated herein by reference, independent just as each document
It is incorporated as with reference to such.In addition, it should also be understood that, after reading the above teachings of the present invention, those skilled in the art can
To make various changes or modifications to the present invention, such equivalent forms equally fall within model defined by the application the appended claims
It encloses.
Claims (18)
1. a kind of artificial light source both culturing microalgae equipment, which is characterized in that the artificial light source both culturing microalgae, which is equipped, includes:
Reaction vessel, the reaction vessel is equipped with cover board and the reaction vessel accommodates the culture solution for micro algae growth；
Charging/discharging device, the charging/discharging device and the reaction vessel are tightly connected；
Light emitting device, the light emitting device are located at reaction vessel interior and when reactor work, the light emitting device portion
Divide or be all immersed in the culture solution, thus light needed for providing micro algae growth in reaction vessel, wherein described shine
The light intensity for the light that device is issued is uniform or substantially uniform in the depth direction of reaction vessel；Wherein, described " uniform
Or substantially uniform " refer to the light intensity D1 of any depth and meet following formula in the average intensity Dm of entire depth range:
1.5≥D1/Dm≥0.7；
Nutrition distribution device, the nutrition distribution device can provide manganese ion into the culture solution；With
Feeder, the feeder includes gas refinement distributor, during being passed through gas into reaction vessel, band
There is the gas of certain pressure uniformly to escape carefully scatteredly by the aperture of the refining device, so that gas be promoted to be scattered in nutriment
In liquid cultivating system；
The gas is the mixture of carbon dioxide and air, and wherein carbon dioxide accounts for the 0.1- of mixed gas total volume
10%；
It is 0.1-1MPa that carbon dioxide, which is passed through pressure,；And/or
It is 0.1-50nm that carbon dioxide, which releases aperture,；
Also, the light emitting device emits the light of at least three different wave lengths when working, be 600-680nm's including wavelength
Light, the light that wavelength is 420-500nm and light that wavelength is 700-780nm.
2. artificial light source both culturing microalgae equipment as described in claim 1, which is characterized in that the manganese ion source in the culture solution
From MnCl2·4H2O, and the MnCl added in every liter of culture solution2·4H2The quality of O is 5-50mg.
3. artificial light source both culturing microalgae equipment as described in claim 1, which is characterized in that
The colour temperature for the complex light that the luminescence unit issues is 1000-20000K.
4. artificial light source both culturing microalgae equipment as claimed in claim 3, which is characterized in that the light packet of three different wave lengths
Include the light that light, wavelength that wavelength is 650-670nm are 440-460nm and the light that wavelength is 710-730nm.
5. artificial light source both culturing microalgae equipment as described in claim 1, which is characterized in that the light emitting device includes light guide plate
And luminescence unit, the luminescence unit are embedded in the light guide plate, the light that the luminescence unit generates makes to lead through light guide plate conduction
Tabula rasa Integral luminous, the light emitting device further include bracket, and the bracket supports the light guide plate for fixed, the bracket with
The reaction vessel and/or the cover board are detachably connected；And/or
In the light emitting device, luminescence unit LED, and the quantity of LED is 1-10000/ light guide plate.
6. artificial light source both culturing microalgae equipment as described in claim 1, which is characterized in that be additionally provided with use in the cultivation equipment
In detecting and controlling the monitoring of carbon dioxide intake and automatic control system in the culture solution, wherein
When the monitoring and automatic control system detect the pH >=5.5 in the culture solution, carbonated mixing
Gas is continually fed into the culture solution；And as the PH in the culture solution < 5.5, pause is passed through two in the culture solution
Carbonoxide.
7. artificial light source both culturing microalgae equipment as described in claim 1, which is characterized in that the artificial light source both culturing microalgae dress
The water quality monitor for monitoring manganese ion concentration in the culture solution, the intake position of the water quality monitor are additionally provided in standby
In liquid level hereinafter, wherein
When the water quality monitor monitors the concentration of the manganese ion in the culture solution lower than default value, the water quality prison
Prompting can be issued by surveying instrument, so that the nutrition distribution device carries out the addition of manganese salt.
8. artificial light source both culturing microalgae equipment as described in claim 1, which is characterized in that the artificial light source both culturing microalgae dress
The standby cultivation for being suitable for fresh water eukaryotic algae.
9. artificial light source both culturing microalgae equipment as claimed in claim 8, which is characterized in that the inoculum density of the algae cultivated
For 0.1g/L-2g/L (algae dry weight/nutrient solution volume).
10. artificial light source both culturing microalgae equipment as described in claim 1, which is characterized in that the light emitting device is issued
Light light intensity it is substantially uniform in the horizontal direction of reaction vessel.
11. artificial light source both culturing microalgae equipment as described in claim 1, which is characterized in that described " uniform or generally uniform
" refer to the light intensity D1 of any depth and meet following formula in the average intensity Dm of entire depth range:
1.2≥D1/Dm≥0.8。
12. artificial light source both culturing microalgae equipment as described in claim 1, which is characterized in that be equipped with temperature around reaction vessel
Device is controlled, the temperature control device is used to maintain the liquid environment temperature in reaction vessel to be in the range for being suitble to micro algae growth
It is interior.
13. artificial light source both culturing microalgae equipment as claimed in claim 12, which is characterized in that the cultivation in the cultivation equipment
Temperature is 10-30 DEG C.
14. artificial light source both culturing microalgae as claimed in claim 12 equipment, which is characterized in that the temperature control device may include
Electric heating or steam-heating system, cold water cooling system.
15. artificial light source both culturing microalgae equipment as described in claim 1, which is characterized in that the artificial light source both culturing microalgae
Equipment is additionally provided with monitoring system, and for monitoring the parameter of liquid environment, parameter is selected from: pH value, temperature and/or nutriment concentration.
16. artificial light source both culturing microalgae equipment as described in claim 1, which is characterized in that the cover board is equipped with outlet
Hole.
17. artificial light source both culturing microalgae equipment as described in claim 1, which is characterized in that the artificial light source both culturing microalgae
30-1000 block light guide plate is equipped in equipment.
18. artificial light source both culturing microalgae equipment as claimed in claim 5, which is characterized in that light guide plate is by with anti-faintly acid
The transparent organic material of energy is made.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510189268.9A CN105316217B (en) | 2015-04-17 | 2015-04-17 | Artificial light source both culturing microalgae equipment |
| PCT/CN2016/079498 WO2016165659A1 (en) | 2015-04-17 | 2016-04-15 | Artificially lighted microalgae farming apparatus |
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| CN201510189268.9A CN105316217B (en) | 2015-04-17 | 2015-04-17 | Artificial light source both culturing microalgae equipment |
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| CN105316217B true CN105316217B (en) | 2019-05-03 |
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| CN105316217B (en) * | 2015-04-17 | 2019-05-03 | 上海希明生物科技有限公司 | Artificial light source both culturing microalgae equipment |
| CN105316235A (en) * | 2015-04-17 | 2016-02-10 | 上海希明生物科技有限公司 | Freshwater eukaryoticmicroalgae culture method |
| CN105733930A (en) * | 2016-05-05 | 2016-07-06 | 中国科学院广州能源研究所 | Rotating disc type photobioreactor for microalgae large-scale culture |
| CN107227252A (en) * | 2017-07-03 | 2017-10-03 | 深圳市华明佰利科技有限公司 | A kind of vertical LED luminous energy biological respinse kettle of totally-enclosed |
| CN112739813A (en) * | 2018-05-03 | 2021-04-30 | 瓦克萨科技有限公司 | Apparatus and method for storing live microalgae |
| CN114438070A (en) * | 2022-02-15 | 2022-05-06 | 广东海纳川生物科技股份有限公司 | Suspended solid microorganism, preparation method thereof, culture pond and application |
| CN114438071A (en) * | 2022-02-15 | 2022-05-06 | 广东海纳川生物科技股份有限公司 | Suspended solid microalgae, preparation method thereof, culture pond and application |
| CN114703051B (en) * | 2022-03-11 | 2024-03-29 | 湖南康大生物药品有限责任公司 | Diatom biological culture system and culture method |
| GB2621138A (en) * | 2022-08-02 | 2024-02-07 | Future By Insects Ltd | Insect production process using carbon capture |
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| CN104195187B (en) * | 2014-08-01 | 2018-02-16 | 润科生物工程（福建）有限公司 | The inverse perfusion of sedimentation releases coupling and prepares method and its special cell settlement groove rich in DHA grease |
| CN105316217B (en) * | 2015-04-17 | 2019-05-03 | 上海希明生物科技有限公司 | Artificial light source both culturing microalgae equipment |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1810978A (en) * | 2005-01-27 | 2006-08-02 | 深圳市东西方生物技术有限公司 | Process of culturing micro algae to produce isotope glucose |
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