CN103555563A - Continuous and automatic harvesting type microalgae mixed breeding device - Google Patents
Continuous and automatic harvesting type microalgae mixed breeding device Download PDFInfo
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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Abstract
The invention relates to a continuous and automatic harvesting type microalgae mixed breeding device which is characterized by comprising a vertical breeding barrel (1) which is made from a transparent material, wherein a flotation machine (4) which vertically rotates and is adjustable in rotary speed is arranged in the center, and the bottom of the flotation machine (4) is communicated with a negative inspiratory pressure bubble generator (6); a liquid inlet (3) is arranged on an upper end cover of the breeding barrel (1); a foam outlet (5) is arranged on the side face at the upper part of the breeding barrel (1); a circle of light sources (2) is arranged around the breeding barrel (1). According to the device provided by the invention, the construction cost of the closed microalgae breeding device is greatly lowered, the transparency of the breeding liquid is enhanced, the extent of full contact between the microalgae and nutrient liquor is further increased, and the growth of the microalgae is promoted. The stability of the dissolved oxygen of the nutrient liquor is improved. The device is convenient to harvest, low in cost, and low in energy consumption. The space is saved, the yield is improved, and breeding and adjustment are easy to control.
Description
Technical field
The present invention relates to a kind of microalgae culturing device adopting, specifically, is a kind of micro-algae mixed breed of type device of automatically gathering continuously.
Background technology
Micro-algae (microalgae) is the unicellular algae existing with individual, chain or group form of a large class microcosmic, and size is not from several microns to hundreds of micron etc.Micro-algae is of a great variety, nearly 200000～800000 kinds according to estimates, wherein has the kind more than 35000 that has recording.According to whether needing the external world to provide organism to support the family in breeding process, micro-algae can be divided into the micro-algae of autotrophy and heterotrophic microalgae, raise together with (hold concurrently and support) micro-algae.From the sixties in 20th century, start to carry out the extensive both culturing microalgae of commercialization abroad, people can realize micro-algae at multiple fields such as protective foods, medical material, beauty treatment, feeds and commercially produce so far.
Current micro-algae large-scale commercial cultivation mainly contains two kinds of modes: open microalgae cultivation system and sealed microalgae cultivating system.Open cultivation system is exactly that the outdoor natural sunlight that utilizes carries out both culturing microalgae, has the advantages such as expansion scale ratio is easier to, cost is lower.But open cultivation system is easily subject to the impact of external environment, as intensity of illumination, light application time, temperature and weather; Also be easily subject to the pollution of other algae kinds, bacterium and pathogenic microorganism.Sealed microalgae cultivating system refers to and utilizes substratum in airtight container, to carry out both culturing microalgae.Sealed microalgae cultivation apparatus can be divided into fermentor tank, culture bag, plate smooth biochemical reactor and cast light biochemical reactor.In fact, the closed reactor adopting in algae industrial production mostly at present is tubular type bioreactor, and such reactor has two portions to form, and main body is the body that communicates with each other and be arranged in parallel, by the good transparent plastics of light transmission or glass, made, frond is accepted illumination ramp at this.Another part is column gas barrier, at this, passes into CO2, adds fresh medium and discharges the O that in circulating system, algal grown produces
2.In order to reduce as far as possible the damage of shearing force to frond, between two portions, conventionally rely on air lift pump to promote nutrient solution circulation.Sealing cultivating system can improve output 60%～300%, and productive rate is higher, applicable various algae kinds, and micro-algae stay in grade, later separation purifying cost also can reduce, and this system is difficult for by living contaminants.But this system device cost is higher, is difficult to expand cultivation scale.Therefore find a kind of high-density, both culturing microalgae method cheaply, very important to promoting both culturing microalgae industrialized development.
Micro-algae is a CB long-pending little (1-30 μ m), density low (close with nutrient solution density), and surface is with the unicellular phototroph of negative charge.But, due to the characteristic of its cell, gather more difficult, cost is high, micro-algae cost of gathering accounts for the 20%-30% of whole industry chain (supply chain).The recovery process of micro algae biomass is a limiting factor of production process.Concentration of algae in normal production is relatively low, be about 0.1g/L-1.0g/L, and frustule is very little, fragile, is vulnerable to damage and breaks.Therefore,, filtration centrifugal with conventional power and natural sedimentation method can not be collected frond effectively.
Summary of the invention
The technical issues that need to address of the present invention are:
(1) sealed microalgae cultivating system is as high in construction costs such as bioreactors at present, inhibited to both culturing microalgae industry.
(2), along with cell density increases, the perviousness variation of light in bioreactor, affects frond growth.
(3) in nutrient solution, the unstable of dissolved oxygen level can suppress frond growth.
(4) in algae liquid, the density of micro-algae is low, causes the cost of gathering high.
(5) to be applied to the centrifuging energy consumption that the micro-algae of industrialization gathers on a large scale too high present stage.And method of enrichment is only applicable compared with macro-algae, as spirulina, and concentration ratio is lower, still needs thereafter centrifugal treating.
For solving the problems of the technologies described above, the present invention takes following technical scheme:
Automatically the micro-algae mixed breed of the type of a gathering device, comprises vertical breeding barrel 1, and described breeding barrel is that light-transmitting materials ，Qi center is provided with vertical rotation and the adjustable air floatation machine 4 of rotating speed, and described air floatation machine 4 bottoms are communicated with the bubble generator 6 of negative inspiratory pressure formula; The upper end cover of described breeding barrel 1 is provided with liquid adding hole 3; Described breeding barrel 1 upper side is provided with row's foam mouth 5; Described breeding barrel 1 is provided with a circle light source 2 around.
When the technical program is implemented, by liquid adding hole 3, the nutritive medium preparing and algae kind are added in breeding barrel 1, outside bucket, surrounding is provided with the cultivation that light source 2 is micro-algae luminous energy is provided, air floatation machine 4 is arranged on the cover plate of breeding barrel 1, air floatation machine 4 drives bubble generator 6 rotations to produce negative pressure, bubble generator 6 is air-breathing from the hollow pipeline of air floatation machine 4, make to drive bubble generator 6 to produce bubble, in the cultivation stage, adopt and produce a small amount of bubble compared with the slow speed of revolution, these bubbles while floating, play two in water body aspect effect: 1. stir water body, algae is rolled in bucket, contact nutritive medium and illumination uniformly when strengthening light transmission, 2. supplement oxygen and the carbonic acid gas consuming due to algal grown in water body, guarantee carrying out smoothly of algae culture.After a culture-cycle finishes, air floatation machine 4 high speed rotating, bubble generator 6 produces a large amount of micro-bubbles, by the most of algae band water-outlet body in water, in water surface, form foam layer, because breeding barrel internal gas pressure is greater than outside, the foam that is therefore rich in micro-algae is discharged from row's foam mouth 5, can use container to collect easily.After micro-algae is collected and finishes, stay algae in water body can be naturally as algae kind to start the next culture-cycle, only need supplement as required the nutritive medium of consumption.
Further, described breeding barrel 1 is cylindric.
Further, the altitude range of described light source 2 is a little more than the height of liquid level in breeding barrel.
Further, described row's foam mouth 5 is above described light source 2.
Further, the bottom surface of described breeding barrel 1 is dome shape.
A kind of method of using above-mentioned micro-algae mixed breed device to carry out both culturing microalgae and gather, comprise cultivation stage and harvest stages, the cultivation stage: by liquid adding hole 3, the nutritive medium preparing and algae kind are added in breeding barrel 1, open light source 2, air floatation machine 4, and air floatation machine 4 is adjusted to compared with the slow speed of revolution; Harvest stages: air floatation machine 4 is adjusted to higher rotation speed, and the foam that is rich in algae of the row's of collection foam mouth 5 discharges, the algae of staying in water body starts the next culture-cycle as algae kind.
Beneficial effect of the present invention is:
1) greatly reduce the equipment construction cost of sealed microalgae cultivation, for both culturing microalgae industry, there is promoter action.
2) by revolving bubble agitation, strengthened the transparence of cultivation liquid, also increased the degree that micro-algae fully contacts with nutritive medium, promoted the growth of frond.
3) revolving bubble has improved the stability of nutrient solution dissolved oxygen.
4) gather conveniently, cost is low.
5) gather and process without centrifuging, consume energy low.
6) saved space, improved output, and cultivated to regulate easily and control.
Accompanying drawing explanation
Fig. 1 is automatically gather the continuously front perspective view of the micro-algae mixed breed of type device of the present invention.
Fig. 2 is automatically gather the continuously vertical view of the micro-algae mixed breed of type device of the present invention.
In figure, 1. breeding barrel, 2. light source, 3. liquid adding hole, 4. air floatation machine, 5. row's foam mouth, 6. bubble generator.
Embodiment
Below in conjunction with the drawings and specific embodiments, the present invention is further described.
Referring to Fig. 1-2, automatically the micro-algae mixed breed of the type of a gathering device, comprises vertical breeding barrel 1, and described breeding barrel is light-transmitting materials, its center is provided with vertical rotation and the adjustable air floatation machine 4 of rotating speed, and described air floatation machine 4 bottoms are communicated with the bubble generator 6 of negative inspiratory pressure formula; The upper end cover of described breeding barrel 1 is provided with liquid adding hole 3; Described breeding barrel 1 upper side is provided with row's foam mouth 5; Described breeding barrel 1 is provided with a circle light source 2 around.
Referring to Fig. 1-2, described breeding barrel 1 is cylindric, and the rotation mixed effect of bubbles entrain algae and nutrient solution is better.
Referring to Fig. 1, the altitude range of described light source 2, a little more than the height of liquid level in breeding barrel, makes algae obtain sufficient illumination, promotes its growth.
Referring to Fig. 1, described row's foam mouth 5 is above described light source 2, and light source is without gap, and it is more reasonable to arrange.
Referring to Fig. 1, the bottom surface of described breeding barrel 1 is dome shape, makes breeding barrel bottom without dead angle, and inner nutrient solution and algae distribution of movement are more even.
A kind of method of using above-mentioned micro-algae mixed breed device to carry out both culturing microalgae and gather, comprise cultivation stage and harvest stages, the cultivation stage: by liquid adding hole 3, the nutritive medium preparing and algae kind are added in breeding barrel 1, open light source 2, air floatation machine 4, and air floatation machine 4 is adjusted to compared with the slow speed of revolution; Harvest stages: air floatation machine 4 is adjusted to higher rotation speed, and the foam that is rich in algae of the row's of collection foam mouth 5 discharges, the algae of staying in water body starts the next culture-cycle as algae kind.
When the present embodiment is specifically implemented, by liquid adding hole 3, the nutritive medium preparing and algae kind are added in breeding barrel 1, outside bucket, surrounding is provided with the cultivation that light source 2 is micro-algae luminous energy is provided, air floatation machine 4 is arranged on the cover plate of breeding barrel 1, air floatation machine 4 drives bubble generator 6 rotations to produce negative pressure, bubble generator 6 is air-breathing from the hollow pipeline of air floatation machine 4, make to drive bubble generator 6 to produce bubble, in the cultivation stage, adopt and produce a small amount of bubble compared with the slow speed of revolution, these bubbles while floating, play two in water body aspect effect: 1. stir water body, algae is rolled in bucket, contact nutritive medium and illumination uniformly when strengthening light transmission, 2. supplement oxygen and the carbonic acid gas consuming due to algal grown in water body, guarantee carrying out smoothly of algae culture.After a culture-cycle finishes, air floatation machine 4 high speed rotating, bubble generator 6 produces a large amount of micro-bubbles, by the most of algae band water-outlet body in water, in water surface, form foam layer, because breeding barrel internal gas pressure is greater than outside, the foam that is therefore rich in micro-algae is discharged from row's foam mouth 5, can use container to collect easily.After micro-algae is collected and finishes, stay algae in water body can be naturally as algae kind to start the next culture-cycle, only need supplement as required the nutritive medium of consumption.
Micro-algae mixed culture can be utilized organism, carbon source, carries out photosynthesis simultaneously.In micro-algae mixed cultivation process, photoautotrophy and chemoheterotrophy are synchronous and relatively independent processes.Illumination is all influential to two pathways metabolisms, but influence degree is different.Chlorella mixed culture technique is cultivated and is compared with light autotrophy training mode with heterotrophism, is the generating mode of a kind of more efficiently production biomass and lipid accumulation, and carbon is effectively recycled in mixed cultivation process, thereby has improved formation efficiency.
Air supporting is to utilize particle or the hydrophobicity of the tensio-active agent of being combined with Particle Phase; make it to be adsorbed on the bubble of artificial generation, and more than rising to liquid level with bubble, form foam layer; again by foam layer and liquid separation, thus the enrichment of realize target material or recovery.The key of By Bubble-floating Method is the generation of microbubble, the present embodiment adopts machinery to become bubble method, by mechanical force, drive bubble generator high speed rotating, in water, form negative pressure automatically air-breathing, and gas is cut into micro-bubble enters into water, thereby the micro-algae enrichment in water is formed to spumescence and discharges breeding barrel, to reach the object of the micro-algae of efficient collection.
The present invention can save space, improves output, avoids the pollution of assorted algae and bacterium, and culture condition easily controls, and is the development trend of micro-algae large-scale industrialized production.It is easy to control that heterotrophism cultivation has culture condition, and in frond, fat content is high; In heterotrophism cultivation, grease accounts for 55% of dry cell weight, far above the fat content 14% of autotrophy; Not climate and illumination effect, frond biomass is large; Can add in batches and improve culture systems with perseverance training method by stream, carry out metabolic regulation.
Claims (6)
1. the micro-algae mixed breed of the type device of automatically gathering continuously, is characterized in that:
Comprise vertical breeding barrel (1), described breeding barrel is that light-transmitting materials ，Qi center is provided with vertical rotation and the adjustable air floatation machine (4) of rotating speed, and described air floatation machine (4) bottom is communicated with the bubble generator (6) of negative inspiratory pressure formula;
The upper end cover of described breeding barrel (1) is provided with liquid adding hole (3);
Described breeding barrel (1) upper side is provided with row's foam mouth (5);
Described breeding barrel (1) is provided with a circle light source (2) around.
2. the micro-algae mixed breed of the type device of automatically gathering continuously as claimed in claim 1, is characterized in that: described breeding barrel (1) is cylindric.
3. the micro-algae mixed breed of the type device of automatically gathering continuously as claimed in claim 1, is characterized in that: the altitude range of described light source (2) is a little more than the height of liquid level in breeding barrel.
4. the micro-algae mixed breed of the type device of automatically gathering continuously as claimed in claim 1, is characterized in that: described row's foam mouth (5) is in the top of described light source (2).
5. the micro-algae mixed breed of the type device of automatically gathering continuously as claimed in claim 2, is characterized in that: the bottom surface of described breeding barrel (1) is dome shape.
6. right to use requires the method that the micro-algae mixed breed device described in 1 carries out both culturing microalgae and gathers, and comprises cultivation stage and harvest stages, it is characterized in that:
The cultivation stage: by liquid adding hole (3), the nutritive medium preparing and algae kind are added in breeding barrel (1), open light source (2), air floatation machine (4), and air floatation machine (4) is adjusted to compared with the slow speed of revolution;
Harvest stages: air floatation machine (4) is adjusted to higher rotation speed, and the foam that is rich in algae of the row's of collection foam mouth (5) discharge, the algae of staying in water body starts the next culture-cycle as algae kind.
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| CN201310567020.2A CN103555563A (en) | 2013-11-14 | 2013-11-14 | Continuous and automatic harvesting type microalgae mixed breeding device |
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| CN201310567020.2A CN103555563A (en) | 2013-11-14 | 2013-11-14 | Continuous and automatic harvesting type microalgae mixed breeding device |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105062868A (en) * | 2015-07-16 | 2015-11-18 | 中国海洋大学 | Apparatus for continuously float-collecting algal cells in raceway pond by microbubbles |
| CN108865651A (en) * | 2018-06-05 | 2018-11-23 | 李盈贤 | A kind of sealing cultivation algae culturing device and its assemble method |
| CN113136315A (en) * | 2021-05-14 | 2021-07-20 | 衢州学院 | Microalgae population culture device and microalgae culture method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1105001A (en) * | 1993-12-28 | 1995-07-12 | 佐藤进 | Gas injection apparatus |
| CN2748464Y (en) * | 2004-05-31 | 2005-12-28 | 中国科学院过程工程研究所 | Air-lift type photo-bioreactor |
| CN101161061A (en) * | 2007-09-07 | 2008-04-16 | 中国水产科学研究院渔业机械仪器研究所 | A system for aquiculture using recirculated water based on decline algae |
-
2013
- 2013-11-14 CN CN201310567020.2A patent/CN103555563A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1105001A (en) * | 1993-12-28 | 1995-07-12 | 佐藤进 | Gas injection apparatus |
| CN2748464Y (en) * | 2004-05-31 | 2005-12-28 | 中国科学院过程工程研究所 | Air-lift type photo-bioreactor |
| CN101161061A (en) * | 2007-09-07 | 2008-04-16 | 中国水产科学研究院渔业机械仪器研究所 | A system for aquiculture using recirculated water based on decline algae |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105062868A (en) * | 2015-07-16 | 2015-11-18 | 中国海洋大学 | Apparatus for continuously float-collecting algal cells in raceway pond by microbubbles |
| CN105062868B (en) * | 2015-07-16 | 2017-07-07 | 中国海洋大学 | A kind of utilization microbubble continues the device that raceway pond frustule is collected in air supporting |
| CN108865651A (en) * | 2018-06-05 | 2018-11-23 | 李盈贤 | A kind of sealing cultivation algae culturing device and its assemble method |
| CN113136315A (en) * | 2021-05-14 | 2021-07-20 | 衢州学院 | Microalgae population culture device and microalgae culture method |
| CN113136315B (en) * | 2021-05-14 | 2023-06-30 | 衢州学院 | Microalgae population culture device |
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Application publication date: 20140205 |