CN205420364U - Little algae system of cultivateing, cavity formula photobioreactor - Google Patents
Little algae system of cultivateing, cavity formula photobioreactor Download PDFInfo
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- CN205420364U CN205420364U CN201521013602.7U CN201521013602U CN205420364U CN 205420364 U CN205420364 U CN 205420364U CN 201521013602 U CN201521013602 U CN 201521013602U CN 205420364 U CN205420364 U CN 205420364U
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Abstract
The utility model discloses a little algae system of cultivateing and cavity formula photobioreactor. The cultivation system is including holding the photobioreactor of culture solution with little algae, the reactor is the closed, the wall for the material of the seal that has cavity sandwich structure and/or wall for lightweight thermal insulation material, the reactor has ventilative structure, contains bicarbonate in the culture solution. Adopt the utility model discloses a little algae is cultivateed by the system, and the controlled reaction ware and the heat exchange between the water environment of locating well makes the cultivation temperature in the reactor be higher than the water temperature of locating to improving the growth rate of little algae, nevertheless being unlikely to the high temperature and leading to the death of algae a little of cultivateing, its cultivation method is simple, and is with low costs, cultivates efficiently, and it is applied to be suitable for the industrialization.
Description
Technical field
This utility model relates to technical field of microalga biology, is specifically related to microalgae culture system, cavity type bioreactor.
Background technology
In the last few years, autotrophy microalgae got growing concern for because of the multiformity of its function.Microalgae can be as a kind of novel biodiesel raw material.Compared with Semen sojae atricolor, Caulis Sacchari sinensis, fiber crops maple equal energy source crop, it has, and growth cycle is short, photosynthesis rate fast, fat content advantages of higher, has good DEVELOPMENT PROSPECT.Microalgae can efficiently fix the CO in air2, solve the environmental problem caused because of combustion of fossil fuel.Additionally, microalgae cell contains: the nutritional labeling of the high values such as protein, lipid, polysaccharide, carotenoid, pigment, it is food and the feedstuff of high-quality, is also the organic intermediate that in chemical industry, light industry and medical industry, purposes is the widest, it is also possible to be cosmetic material.
As other biology, temperature is the most important factor affecting micro algae growth.In traditional microalgae culture system, in long-time strong illumination can cause closed photo bioreactor, temperature can raise 10 DEG C～30 DEG C, and too high temperature can cause microalgae yield to decline, and even causes the death of microalgae.Therefore, efficient microalgae culture system needs extra temperature control system, as spray water is lowered the temperature, but which results in the high cost that microalgae produces.In order to avoid this situation, researcher is had to develop the microalgae culture system being soaked in water or swimming on the water surface, such as, the microalgae culture system being soaked in water of U.S. Solix company exploitation, the floatation type microalgae culture system of Lee et al. invention and the floatation type semipermeable membrane microalgae culture system etc. of NASA's invention.
But, above-mentioned floatation type microalgae culture system commercial applications has nevertheless suffered from limiting.First, although residing water body can reduce the temperature in reactor so that it is temperature is unlikely to rise to too high and cause microalgae cell dead, but also the temperature in reactor is reduced to the temperature almost identical with residing water body simultaneously.Spontaneous evaporation effect due to water body, its temperature is the lowest, such as, in the marine site of China, the Bohai Sea four seasons average sea surface temperature is respectively 2.8 DEG C, 12.7 DEG C, 23.5 DEG C and 13.5 DEG C, four season of the Huanghai Sea, average sea surface temperature was respectively 8.4 DEG C, 14.9 DEG C, 24.1,17.1 DEG C, the East Sea four seasons average sea surface temperature is respectively 16.8 DEG C, 21.7 DEG C, 28.0 DEG C and 23.0 DEG C, and the South Sea four seasons average sea surface temperature is respectively 25.1 DEG C, 28.2,29.1 DEG C and 27.1 DEG C.Too low temperature is unfavorable for the growth of microalgae, and the growth efficiency causing microalgae is low.Such as, super addicted to the salt bar algae speed of growth significantly different (table 1) at different temperatures.Therefore, the cultivation temperature in reactor is improved most important.
Table 1. surpasses the optical density cultivated at different temperatures 5 days addicted to salt bar algae
It addition, the supply of inorganic carbon source is most important for microdisk electrode, and how reactor for floatation type provides inorganic carbon source to be a difficult problem.As above-described floatation type Photoreactor, using closed system, and be bubbled into the compressed gas containing carbon dioxide wherein, this is difficulty with for large-scale culturing micro-algae in fact.This tubing needing to install a bubble aeration for each Photoreactor.In general, it is very big that the size of each floating reactor can not be done, and this reactor being accomplished by vast number carries out large-scale culture.In this case, the bubble aeration of the reactor of vast number needs extremely complex compressed gas pipeline, this considerably increases cost of manufacture and the construction cost of compressed gas pipeline of each reactor, can be greatly increased running cost simultaneously.And set up so complicated breather line at large-area water surface, it is bigger that it builds difficulty, makes and running cost is higher, and its by wave destroy very risky, so, in large-scale production, this is very difficult to realize.And if it is considered that utilize the reactor assembly opened wide, although eliminating bubble aeration system, and beneficially carbon dioxide is delivered in culture fluid from air, thus absorbed by microalgae, but open system is due to the effect of evaporation, and its temperature temperature difference original with water body is very few, it is impossible to formation greenhouse effect, therefore micro algae growth is slow, and efficiency is the lowest.
Utility model content
In view of not enough present in microdisk electrode device and microdisk electrode in prior art, one of the purpose of this utility model is to provide a kind of system for microdisk electrode, uses reasonably design, can Effective Regulation system temperature.
The technical solution of the utility model is, microalgae culture system, including the bioreactor that can accommodate culture fluid and microalgae；Described reactor is closed；The wall of described reactor be the material of the seal and/or wall with hollow sandwich structure be light heat insulation material；Described reactor has ventilating structure；Containing bicarbonate in described culture fluid.
" lightweight " of light heat insulation material described in the utility model refers to floatability on the water surface.
This utility model bioreactor uses light heat insulation material to make so that it is can swim on the water surface；When using non-light material, can have the wall of hollow sandwich structure to arrive floating effect by making.
In this utility model microalgae culture system, bicarbonate provides inorganic carbon source for microalgae so that it is grow in bioreactor.Oxygen produced by microalgae photosynthesis is discharged by the sealing structure (such as, have the diaphragm seal of permeable watertight characteristic) with permeable watertight, or is discharged by ventilation mouth.
The microalgae culture system that this utility model provides, is applied to the wavy water surface of tool opened wide, and described water body may be, but not limited to, ocean, lake, river, pond or reservoir.
nullMicroalgae described in the utility model is any eucaryon microalgae that can grow under the conditions of certain magnesium hydrogen salt concentration and cyanobacteria，Such as，Described microalgae can be selected from Dunaliella salina (Dunaliellasp.)、Chlorella (Chlorellasp.)、Haematocoocus Pluvialls (Hematococcussp)、Super addicted to salt bar algae (Euhalothecesp.)、Blue bar algae (Cyanothecesp.)、Spirulina (Spirulina.)、Micro-sheath algae (Microcoleussp.)、Cytoalgae (Synechocystissp.)、Isochrysis galbana (isochrysissp)、Little determine whip chrysophyceae (Prymnesiumsp)、The new chlorella of rich oil (Neochlorisoleoabundans)、Micro-plan ball algae (Nannochloropsis)、Or the microalgae of a kind of entitled Picochlorumsp of Latin.
The microalgae culture system that this utility model provides, its Photoreactor is while providing buoyancy, also can reduce the heat exchange between inside reactor and residing water body, form a floatation type greenhouse under sunlight irradiates, the microdisk electrode temperature of maintenance is higher than the water temperature residing for Photoreactor.
1) this culture systems is placed in wavy water surface, utilizes the energy of wave to realize the mixing of culture fluid in reactor, to meet the mass transfer requirement of micro algae growth.
2) in this culture systems, the wall of Photoreactor uses the seal with hollow sandwich structure to make, or use light heat insulation material to make, while buoyancy is provided, reduce the heat exchange between inside reactor and residing water body, forming a floatation type greenhouse under sunlight irradiates, the microdisk electrode temperature of maintenance is higher than the water temperature residing for Photoreactor.
3) reaching heat insulation effect, Photoreactor uses the inorganic carbon source needed for the design closed, micro algae growth to provide with the form of bicarbonate, to avoid utilizing the carbon dioxide in air or being passed through the heat loss that the gas containing carbon dioxide causes.
Another object of the present utility model is to provide a kind of cavity type bioreactor, and including cavity, cavity has cavity wall, and cavity wall includes cavity wall；Described cavity wall be the material of the seal and/or cavity wall with hollow sandwich structure be light heat insulation material；The top of described cavity is provided with feed inlet and outlet and ventilating structure, and described feed inlet and outlet and ventilating structure both pass through cavity wall and communicate with inside cavity.
According to the difference of cavity wall material, cavity type bioreactor can be rigidity or flexibility.
Feed inlet and outlet may be used without the sealing structure of permeable watertight.Preferably the membrane material of this permeable watertight is expanded PTFE.On the one hand it be possible to prevent water body to enter inside reactor, pollutes microalgae.On the other hand, can appear with the oxygen that photosynthesis produces, reduce the accumulation of dissolved oxygen.
Temperature is the key factor of micro algae growth, and temperature is too low, and micro algae growth is slow, and temperature is too high, the most easily causes the death of microalgae cell, therefore, controls microdisk electrode temperature most important.But it is unpractical for utilizing the means such as electrical heating or logical cooling water to control temperature.
For outdoor microalgae culture system, the dynamic equilibrium between the heat that the temperature in reactor is the heat inputted by sunlight and reactor outwardly spreads out of determines.Open water temperature is relatively low, can be reduced the temperature in culture systems by heat transfer, and the temperature identical with environment water is generally not the preferable temperature of micro algae growth, the too low growth being unfavorable for microalgae of temperature.
The cavity type bioreactor that this utility model provides, its cavity wall uses the seal with hollow sandwich structure, fills gas, such as air in interlayer.On the one hand, hollow sandwich structure, not only for the reactor floating offer buoyancy on the water surface, due also to the heat conductivity of air is the lowest, can play heat insulation effect；On the other hand, hollow sandwich structure also helps and avoids the evaporation due to culture fluid to form water smoke on cavity wall, it is ensured that the efficient entrance of light, improves sufficient light source for micro algae growth.
As another embodiment, the cavity wall of this reactor uses light heat insulation material to make, and this equally simultaneously works as insulation and provides the effect of buoyancy.Heat insulation effect can be controlled by the material and thickness thereof that adjust cavity wall.Such as, can use in the winter time the material of better heat preservation and the bigger reactor of cavity wall thickness to cultivate microalgae, to maintain the temperature difference bigger inside and outside reactor；And in summer, when water temperature is higher, the material that heat conductivity is bigger can be used, and reduce the thickness of reactor cavity wall, to maintain a less reactor internal-external temperature difference, prevent the too high injury to microalgae caused of temperature.
Therefore, the cavity type bioreactor reactor that this utility model provides, it is placed in the water body surface such as (sea, river surface, lake surface, pond, reservoir water body) in field, can be still that providing of microalgae is beneficial to temperature environment its growth, relatively stable.
The photosynthesis of microalgae can produce oxygen, if escaped in time in the oxygen not gas phase in reactor, can cause oxyty too high in culture fluid, and this can have a strong impact on the growth of microalgae cell, even results in death.Therefore, in the design of reactor of the present utility model, it is provided with ventilating structure, escapes with oxygen.
As preferred technical scheme, described ventilating structure includes the sealing structure of ventilation mouth and/or permeable watertight.
Ventilation mouth typically uses small-bore, it is preferable that its ventilation mouth is a diameter of less than 0.5cm.
The ventilating structure being preferably used is ventilative but fluid-tight diaphragm seal, so while the oxygen that effectively release photosynthesis produces, it is ensured that the sealing of whole reactor, is unlikely to cause the loss of culture fluid.Meanwhile, under outdoor environment, rainwater can be avoided to enter reactor.Further, since the sealing structure of permeable watertight maintains an enclosed environment, the pollutant such as dust in air and heavy metal particles also cannot be introduced into reactor.Antibacterial, fungus, the microorganism such as microalgae also cannot be introduced into, and wheel animalcule, artemia etc., with the biology etc. of microalgae of ingesting, all cannot enter inside reactor by this film, can be effectively prevented from these and pollute.
As preferred technical scheme, the material of described seal is transparent material, makes sunlight the most how to be input in reactor, provides light and heat for micro algae growth.
As preferred technical scheme, the material of described seal can be, but is not limited to PVC (polrvinyl chloride), PP (polypropylene), PE (polyethylene) or the plastic such as PA (polyamide), PMMA (polymethacrylates).
As preferred technical scheme, described insulation material is foam plastics.
As preferred technical scheme, described foam plastics can be, but is not limited to PVC (polrvinyl chloride), PP (polypropylene), PE (polyethylene) or PA (polyamide), PMMA (polymethacrylates) or other modified foam article.
As preferred technical scheme, described cavity wall is made up of upper cavity wall, lower cavity wall and side chamber wall, and described side chamber wall in the vertical direction is made up of at least two hollow sealing unit I, seals overlapping between described hollow sealing unit I.
Relative to single layer structure, this hollow sealing unit I seals the side wall construction being formed by stacking, has more preferable balanced capacity when wave stroke, contribute to stablizing of reactor.The cavity wall hollow sealing unit I forming reactor is preferably square cylinder structure.What is more important, the design of multilamellar absolute construction is possible to prevent leak gas because of certain absolute construction or rupture the sinking of the whole reactor caused.
As preferred technical scheme, the outer layer of described hollow sandwich structure is provided with inflation inlet, also referred to as valve, in order to fill gas, provides buoyancy for reactor.
As preferred technical scheme, described upper cavity wall in the horizontal direction, is made up of at least two hollow sealing unit II, is tightly connected between described hollow sealing unit II.This structure is conducive to maintaining 3D shape and the structure of reactor, is more beneficial for preventing the generation of water smoke, it is ensured that the entrance of light.
As preferred technical scheme, described upper cavity wall is made up of the thin film of the pvc material with anti-fog function, antifog while, strengthen incident illumination intensity.
As preferred technical scheme, the bottom of described cavity connects stable parts, and described parts of stablizing are rope or pipeline configuration；One end of described pipeline configuration connects with the inside of described cavity, and the other end is provided with detachable-type sealed structure.
When microalgae culture system provided by the utility model swims on the water surface, wave can be that the mixing of reactor provides power, relative to the agitating device of traditional electrical energy drive, the power that wave provides is the most free, and this can significantly decrease energy consumption and cost.But, while wave provides mixing power, it is also possible to cause toppling of reactor, therefore, take bottom to connect the design having stable parts, reactor stability on the water surface can be increased.
As the improved structure of rock-steady structure, the rope of certain length is installed in the bottom of cavity, and falls with weight, to increase the stability of reactor.
Improved structure as another kind of rock-steady structure, it is also possible to install the pipeline configuration of certain length in the bottom of reactor cavity, improves the Stabilization to reactor.One end of pipeline configuration connects with the inside cavity of described bioreactor, and the other end is provided with detachable-type sealed structure, as sealed lid.
Above-mentioned cavity type bioreactor collects microalgae, forms microalgae concentrated solution with described pipeline configuration sedimentation microalgae.After microdisk electrode terminates, the results of its gained cell are always the most extremely difficult problem.Such as, in open cell system, usual cell density, less than 1g/L, if realized the separation of microalgae cell by means that are centrifugal or that filter, needs substantial amounts of energy consumption and cost.When using reactor of the present utility model, the algae solution cultivated can enter in pipeline, and settles in this pipeline, finally forms microalgae concentrated solution bottom flexible pipe, and this can significantly facilitate the results process of microalgae, reduce energy consumption and cost.
The material of described pipeline configuration is not particularly limited to, and can use the materials such as rustless steel, PVC (polrvinyl chloride), PP (polypropylene), PE (polyethylene) or PA (polyamide).
As preferred technical scheme, described hollow sandwich structure is filled gas, such as air.
Above-described microalgae culture system or above-described cavity type bioreactor is used to cultivate microalgae, microalgae and the micro algae culturing liquid containing solubility bicarbonate are placed in described cavity type bioreactor, then this cavity type bioreactor is placed in wavy water surface, the energy of wave is utilized to realize the mixing of culture fluid in reactor for chief motivation, to meet reactor inner transmission matter requirement.
Microdisk electrode needs carbon source, current culture systems to normally bear against the gas being passed through in reactor containing carbon dioxide or the carbon dioxide utilized in air realizes.Being passed through the gas containing carbon dioxide is that a very effective carbon dioxide provides means, but, for large-scale microdisk electrode, for each Photoreactor, the unpractical of one carbon dioxide aerating system is installed.And utilize the carbon dioxide in air, it is necessary to use open by design, be beneficial to carbon dioxide from gas phase from the transmission of liquid phase.
For industrialization, large-scale closed culture systems, above two means are the most infeasible.For solving this problem, culture systems provided by the utility model utilizes bicarbonate to provide carbon source for micro algae growth.Described solubility bicarbonate is at least one in the solubility bicarbonates such as sodium bicarbonate, potassium bicarbonate, calcium bicarbonate, ammonium hydrogen carbonate.Toleration and the production of magnesium hydrogen salt concentration can be actually needed and select according to microalgae by solubility bicarbonate concentration in micro algae culturing liquid, and its concentration can be to saturated concentration from extremely low concentration.Described solubility bicarbonate concentration in micro algae culturing liquid is that 0.01mol/L is to its saturated concentration.
In the range of can tolerating, magnesium hydrogen salt concentration is the highest, and it supports that the possible density of microalgae cell growth is the highest.Such as, if the bicarbonate containing 0.1mol/L in micro-algae culture medium, if these bicarbonates would all be consumed, it is provided that the DIC of 1.2g/L.As a rule, containing the carbon of about 50% in the dry weight of microalgae biomass, therefore, the carbon of this 1.2g/L can be for the micro algae growth of about 2.4g/L in theory.And, a lot of microalgae can grow under the bicarbonate environment of higher concentration, such as, surpasses and can grow in saturated sodium bicarbonate addicted to salt bar algae, and this concentration is already above 1mol/L, and this can be that its growth provides the most sufficient carbon source.
When magnesium hydrogen salt concentration is higher, can select addicted to saline and alkaline microalgae, such as, Dunaliella salina (Dunaliellasp.), chlorella (Chlorellasp.), super addicted to salt bar algae (Euhalothecesp.), blue bar algae (Cyanothecesp.), spirulina (Spirulinasp.), micro-sheath algae (Microcoleussp.), cytoalgae (Synechocystissp.) etc., these algae have good toleration for the bicarbonate of high concentration.It should be noted that this utility model is applicable to the microalgae that any one can grow under certain magnesium hydrogen salt concentration.
The beneficial effects of the utility model:
This utility model provides microalgae culture system and cavity type bioreactor.
Microalgae culture system floatability of the present utility model in water surface, utilizes the energy of wave to realize the concussion of culture fluid mixing microalgae in system and cultivates.
Cavity type bioreactor of the present utility model, accept illumination and support micro algae growth, utilize illumination improve the reactor temperature design by reactor cavity wall and make the heat exchange controlling between reactor and residing water body environment simultaneously, make the cultivation temperature in reactor higher than residing water temperature, to improve the speed of growth of microalgae, but it is too high and cause the death of cultivated microalgae to be unlikely to temperature:
1, for avoid too much thermal loss, the cavity wall of this structure of reactor uses hollow sandwich design, and to reduce the heat exchange between wall and environment, the most this structure stabilizes it again and swims in water surface offer buoyancy.
2, this reactor uses the design closed, to reduce gas exchange and to evaporate the heat loss caused.
3, sidewall design becomes at least more than one hollow sealing unit and overlaps, and is provided with rock-steady structure bottom reactor cavity, to improve floatability and the stability of reactor.Particular, it is important that it is prevented from leaking gas because of certain absolute construction or rupturing the sinking of the whole reactor caused.
Utilize the pipeline configuration connected with cavity type reactor lower wall in this utility model, microalgae concentrated solution can be formed in the duct, significantly facilitate the collection of microalgae.
The microalgae culture system of this utility model offer, cavity type bioreactor, use bicarbonate as carbon source, on the one hand carbon source required for micro algae growth is provided, avoid bubble type to provide the aerating system brought of carbon source complicated and reactor that is that cause makes difficulty and high cost simultaneously, be also avoided that the high energy consumption problem of ventilation.
Accompanying drawing explanation
Fig. 1 cavity type bioreactor is used for microdisk electrode schematic diagram；
Fig. 2 cavity type bioreactor structural representation；
Fig. 3 rigidity cavity type bioreactor front view；
Fig. 4 rigidity cavity type bioreactor top view；
Fig. 5 flexibility cavity type bioreactor front view；
Fig. 6 flexibility cavity type bioreactor top view；
Fig. 7 wall material is the bioreactor front view of light heat insulation material；
Fig. 8 wall material is the bioreactor top view of light heat insulation material；
Surpassing addicted to salt bar algae growth curve of Fig. 9 rigidity cavity type bioreactor indoor cultivation；
The Dunaliella salina algae growth curve of Figure 10 rigidity cavity type bioreactor indoor cultivation；
Surpassing addicted to salt bar algae growth curve of Figure 11 rigidity cavity type bioreactor outside scenery；
Figure 12 water body and Flexible reactor (PVC material) interior culture-liquid temp change ((24) hour) of different polycrystalline substance；
Figure 13 water body and Flexible reactor (PVC material) interior culture-liquid temp change (12 days) of different polycrystalline substance；
Surpassing addicted to salt bar algae growth curve of Flexible reactor (PVC material) outside scenery of Figure 14 difference polycrystalline substance；
In figure, 1 cavity；2 cavity wall；Cavity wall on 21；22 times cavity wall；23 side chamber walls；24 hollow sealing unit I；25 hollow sealing unit II；3 hollow sandwich structures；4 inflation inlets；5 feed inlet and outlets；6 ventilation mouths；The sealing structure of 7 permeable watertights；8 stablize parts；9 detachable-type sealed lids；
A curve: reactor 1#, liquid level 5 centimetres；
B curve: reactor 1#, liquid level 10 centimetres；
C curve: reactor 2#, liquid level 5 centimetres；
A curve: the reactor at the bottom of double-layer inflatable；
B curve: the reactor at the bottom of monolayer；
C curve: water temperature.
Detailed description of the invention
Following non-limiting example can make those of ordinary skill in the art that this utility model is more fully understood, but limits this utility model never in any form.The description below is only the exemplary illustration to the scope that this application claims, and the utility model of the application can be made multiple change and modification according to disclosure of that by those skilled in the art, and it should also be as belonging among the scope that this application claims.
Embodiment 1 cavity type bioreactor
As shown in Figure 1 and Figure 2, this reactor includes cavity 1, and the cavity wall 2 of cavity 1 is the seal with hollow sandwich structure 3, and hollow sandwich structure 3 outer layer is provided with inflation inlet 4.Cavity wall 2 is made up of upper cavity wall 21, lower cavity wall 22 and side chamber wall 23 cavity 1.
The top of cavity 1 is respectively equipped with feed inlet and outlet 5 and ventilation mouth 6, feed inlet and outlet 5 and ventilation mouth 6 and both passes through that cavity wall 2 and cavity 1 are internal to be communicated.During use, put into the material for cultivating by feed inlet and outlet 5, such as microalgae and micro algae culturing liquid, and in the incubation of microalgae, also by this feed inlet and outlet, sampling, observe the growing state of microalgae.Feed inlet and outlet 5 and ventilation mouth 6 are provided with the sealing structure 7 of permeable watertight, and it has the membrane material of permeable watertight.On the one hand, the oxygen that this membrane material can produce through photosynthesis, on the other hand, make reactor be in the state of closing, to reduce gas exchange and to evaporate the heat loss caused, improve the temperature in reactor.
The bottom of cavity 1 connects stable parts 8, and stablizing parts 8 is pipeline configuration, and one end of pipeline configuration connects with the inside cavity of bioreactor, and the other end is provided with detachable-type sealed structure 9.In use, the part in the algae solution cultivated can enter in this pipeline, and cell settles in this pipeline, finally forms microalgae concentrated solution bottom flexible pipe, and this can significantly facilitate the results process of microalgae, reduce energy consumption and cost.
The material preferably clear material of cavity wall 2, can use silicate glass；Lucite；Plastics such as polrvinyl chloride (PVC), polypropylene (PP), polyethylene (PE) or polyamide (PA) etc..Cavity geometry can use the shapes such as flat, flat, pillar, tubular type, pocket type, wherein preferred quad flat structure, and this structure is beneficial to its stability and strengthens the mass transfer of culture fluid, and, be conducive to increasing its area accepting illumination.
Embodiment 2 rigidity cavity type bioreactor
As shown in Figure 3, Figure 4, this reactor includes cavity 1, and the cavity wall 2 of cavity 1 is formed by connecting through bonding by upper cavity wall 21, lower cavity wall 22 and side chamber wall 23 by cavity 1.Side chamber wall 23 and lower cavity wall 22 are the seal with hollow sandwich structure 3.The top of cavity 1 is respectively equipped with feed inlet and outlet 5 and ventilation mouth 6, feed inlet and outlet 5 and ventilation mouth 6 and both passes through that cavity wall 2 and cavity 1 are internal to be communicated.
During use, put into the material for cultivating by feed inlet and outlet 5, such as microalgae and micro algae culturing liquid, and in the incubation of microalgae, also by this feed inlet and outlet, sampling, observe the growing state of microalgae.Feed inlet and outlet 5 and ventilation mouth 6 are provided with the sealing structure 7 of permeable watertight, and it has the membrane material of permeable watertight.In incubation, on the one hand, the oxygen that this membrane material can produce through photosynthesis, on the other hand, make reactor be in the state of closing, to reduce gas exchange and to evaporate the heat loss caused, improve the temperature in reactor.
The rigidity cavity type bioreactor made in the present embodiment, it is shaped as square, and cavity wall 2 is the lucite of the high transparency with hollow sandwich structure 3, and thickness of glass is 1.0cm, and hollow sandwich thickness is 2.0cm.In this way, making bioreactor 1# and 2#, the wherein a length of 25.0cm of reactor 1#, width is 22.7cm, and height is 14.0cm, a length of 50.0cm of reactor 2#, and width is 50.0cm, and height is 12.0cm.
The flexible cavity type bioreactor of embodiment 3
As shown in Figure 5, Figure 6, this reactor includes cavity 1, and the cavity wall 2 of cavity 1 is made up of upper cavity wall 21, lower cavity wall 22 and side chamber wall 23 cavity 1.Cavity wall 2 is polrvinyl chloride (PVC) thin film that transparency is the highest.
Side chamber wall 23 is the seal with hollow sandwich structure 3, in the vertical direction, is made up of 3 hollow sealing unit I 24, seals overlapping between hollow sealing unit I 24, and each hollow sealing unit I is designed with for inflatable and deflatable inflation inlet 4.
Upper cavity wall 21 is the seal with hollow sandwich structure 3, in the horizontal direction, is made up of several hollow sealing unit II 25, is tightly connected between described hollow sealing unit II 25.The top of cavity 1 is respectively equipped with feed inlet and outlet 5 and ventilation mouth 6, feed inlet and outlet 5 and ventilation mouth 6 and both passes through that cavity wall 2 and cavity 1 are internal to be communicated.
Having made rectangular bioreactor 3# and 4# of two a size of 40 × 40 × 20 in the present embodiment, its difference is: the bottom of reactor 3# is monolayer, is sandwich bottom reactor 4#, during use, is filled with air, inflation thickness for 2.0cm.
Embodiment 4 wall material is the cavity type bioreactor of light heat insulation material
As shown in Figure 7, Figure 8, this reactor includes cavity 1, upper cavity wall 21, lower cavity wall 22 and side chamber wall 23.The top of cavity 1 is respectively equipped with feed inlet and outlet 5 and ventilation mouth 6, feed inlet and outlet 5 and ventilation mouth 6 and both passes through that cavity wall 21 and cavity 1 are internal to be communicated.Wherein descending cavity wall 22 and side chamber wall 23 to be the polyethylene foamed plastics of light thermal-insulation, thickness is 3.0 centimetres.Upper cavity wall 21 material, preferably has the polrvinyl chloride of anti-fog function, and thickness is 0.1 centimetre.The present embodiment has made the rectangular bioreactor 5# of a size of 40 × 40 × 20.
Embodiment 5 rigidity cavity type bioreactor indoor cultivation microalgae
Utilizing bioreactor 1# and 2# described in embodiment 2, super addicted to salt bar algae and Dunaliella salina at indoor cultivation, its culture medium prescription is as follows: wherein table 2 is for super addicted to salt bar algae culture medium, and table 3 is Dunaliella salina culture medium.
Table 2 surpasses addicted to salt bar algae culture medium
Table 3 Dunaliella salina culture medium
Above-mentioned culture medium prescription components in certain proportion is dissolved in distilled water, obtains micro algae culturing liquid.The super cultivation addicted to salt bar algae: add this micro algae culturing liquid in Photoreactor 1#, until reaching the liquid layer thickness of 10.0cm, choosing the super of exponential phase to inoculate with 10% rate of vaccination addicted to salt bar algae (EuhalotheceZM001), Initial seeding density is respectively 0.20g/L.Having wavy pool inner water temperature constant is 20 ± 1 DEG C, cultivates under indoors artificial light source, and light intensity is constant is 300 μm olE/m2/s。
The cultivation of Dunaliella salina: add this micro algae culturing liquid in Photoreactor 1#, until reaching the liquid layer thickness of 10.0cm, and choose the Dunaliella salina of exponential phase, with 10% rate of vaccination inoculation, it is 20 ± 1 DEG C that Initial seeding density has wavy pool inner water temperature constant for respectively 0.10g/L., cultivating under indoors artificial light source, light intensity is constant is 300 μm olE/m2/s。
Starting cultivation, every day, dry weight surveyed by timing sampling.Survey dry weight method as follows:
Accurately measuring algae solution 40ml, 10000rpm is centrifuged 10 minutes and collects frustule, and the sodium-chloride water solution 40mL measuring 10g/L cleans the frustule collected, and is repeated twice.The frustule finally collected adds in the sodium-chloride water solution of 5mL, dries to constant mass, weigh frustule dry weight with analytical precision balances, and calculate the dry weight of microalgae at 105 DEG C.
Result: as shown in Fig. 9, curve in 10, photoproduction sundries of the present utility model can be cultivated super addicted to salt bar algae and Dunaliella salina.Wherein, super addicted to salt bar algae when the 9th day, cell concentration is maximum, concentration C nowx=2.29g/L.And when the 4th day, its volume productivity was maximum, for 0.61g/L/d.It is 0.72g/L that Dunaliella salina obtained maximal cell concn when the 7th day.It is seen from the above data that the microalgae using bioreactor of the present utility model to cultivate under culture conditions can reach higher cell concentration.
Embodiment 6 rigidity cavity type bioreactor outside scenery microalgae
Utilizing bioreactor 1# and 2# described in embodiment 2, super addicted to salt bar algae at outside scenery, the preparation of its culture medium prescription and culture fluid is with embodiment 4.Any artificial treatment is not done in temperature and illumination, is completely dependent on natural conditions.
Starting cultivation, every day, dry weight surveyed by timing sampling.Survey dry weight method such as embodiment 2.
Result: as shown in Figure 11, bioreactor 1# and 2# of the present utility model, all can successfully cultivate super addicted to salt bar algae under outdoor conditions.The maximal cell concn position successively that wherein reactor 1 and 2 is cultivated, 1.47g/L, 1.28g/L (C and B in the most corresponding Figure 11).As shown in the A in Figure 11, its Cmax 0.95g/L.
Conclusion: it is seen from the above data that use the microalgae cultivated under bioreactor of the present utility model and condition of culture can reach higher cell concentration, and its energy consumption, cultivation, running cost are low.
The flexible cavity type bioreactor outside scenery microalgae of embodiment 7
Utilize bioreactor 3# and 4# described in embodiment 3, super addicted to salt bar algae at outside scenery.Floating conditions and reactor temperature measure: floated on by the reactor made in the outdoor wavy pond of tool, this micro algae culturing liquid is added in 3 and 4 in above-mentioned Photoreactor, and choose the EuhalotheceZM001 of exponential phase, Initial seeding density is 0.20g/L, in this case, inside reactor and the temperature of water body residing for it, light intensity are measured respectively in early 7 changes in the time period up to late 19 time.
As seen from Figure 12, in 7 .-13 time range, due to the irradiation of sunlight, culture-liquid temp is gradually increased, and reaches maximum when 13, is 29 DEG C.On the other hand, the water temperature in pond also can raise, and unlike in reactor, it to maximum temperature, is 23.9 DEG C when 15, and less than the culture-liquid temp of reactor, this is because specific heat of water is molten very big, therefore water temperature changes little.It should be noted that in the range of the experimental period of a day, all high than the temperature of residing water body in floatation type greenhouse reactor, and when the maximum temperature difference between them occurs in 13, be 5.7 DEG C, illustrate that bioreactor of the present utility model has preferable heat insulating ability.
As shown in figure 12, the temperature in monolayer reactor can be higher than coolant-temperature gage, but temperature difference is between-0.1 to 0.7 DEG C, the most great significant difference.Compared with the reactor bottom monolayer, there is the reactor bottom air blanketing, bigger temperature difference (5.7 DEG C) can be formed.Bottom has the reactor of inflatable structure and water temperature has bigger temperature contrast to hinder more greatly the transmission of heat due to the thermal resistance of the air layer of inflation, thus keeps higher temperature.It is noted herein that, as shown in figure 13, there is the reactor bottom air blanketing (daytime) in whole cultivation cycle and all maintain the temperature higher than the culture fluid in water body and monolayer reactor, illustrate that reactor of the present utility model has preferable heat insulation function, and more stable.And this temperature difference can be realized by the thickness of regulation air layer and the ratio accounting for whole floor space, can be realized the regulation and control of reactor temperature by the design of reactor bottom with this.But.In actual production, using which kind of reactor to reflect the algae kind depending on cultivating, different algae kinds has different suitable growth temperature ranges.
Surpassing addicted to salt bar algae selected by the present embodiment is a kind of microalgae with higher optimum growth temperature, and higher temperature is beneficial to its growth.Therefore, the reactor with heat insulation function can promote that it grows.As shown in figure 14, the final Biomass of microalgae of reactor 4# reaches 0.89g/L, and the final Biomass of reactor 3# only has 0.67g/L.It follows that the reactor of the present embodiment, microalgae can be cultivated in outdoor, and there is heat insulation function, and the growth of microalgae can be promoted.
Conclusion: it is seen from the above data that use the microalgae cultivated under the bioreactor of the present embodiment and condition of culture can reach higher cell concentration, its heat insulation effect is good, and cell can be maintained to grow faster.
Claims (21)
1. microalgae culture system, including the bioreactor that can accommodate culture fluid and microalgae；It is characterized in that,
Described reactor is closed；
The wall of described reactor be the material of the seal and/or wall with hollow sandwich structure (3) be light heat insulation material；
Described reactor has ventilating structure；
Containing bicarbonate in described culture fluid.
Microalgae culture system the most according to claim 1, it is characterised in that described ventilating structure includes the sealing structure (7) of ventilation mouth (6) and/or permeable watertight.
Microalgae culture system the most according to claim 1, it is characterised in that the material of described seal is transparent material.
Microalgae culture system the most according to claim 1, it is characterised in that the material of described seal is macromolecule polymer material.
Microalgae culture system the most according to claim 4, it is characterised in that described macromolecule polymer material is polrvinyl chloride, polypropylene, polyethylene, polymethacrylates or polyamide.
Microalgae culture system the most according to claim 1, it is characterised in that described light heat insulation material is foam plastics.
Microalgae culture system the most according to claim 6, it is characterised in that described foam plastics is polrvinyl chloride, polypropylene, polyethylene, polymethacrylates or polyamide.
Microalgae culture system the most according to claim 1, it is characterised in that fill gas in described hollow sandwich structure (3).
Microalgae culture system the most according to claim 1, it is characterised in that described system is applied to have wavy water surface, and described water body is ocean, lake, river, pond or reservoir.
10. cavity type bioreactor, it is characterised in that including that cavity (1), cavity (1) have cavity wall (2), cavity wall (2) includes cavity wall (21)；Described cavity wall (2) is for having the seal of hollow sandwich structure (3) and/or the material of cavity wall (2) is light heat insulation material；The top of described cavity (1) is provided with feed inlet and outlet (5) and ventilating structure, and described feed inlet and outlet (5) and ventilating structure both pass through cavity wall (21) and communicate with cavity (1) inside.
11. cavity type bioreactors according to claim 10, it is characterised in that described ventilating structure includes the sealing structure (7) of ventilation mouth (6) and/or permeable watertight.
12. cavity type bioreactors according to claim 10, it is characterised in that the material of described seal is transparent material.
13. cavity type bioreactors according to claim 10, it is characterised in that the material of described seal is macromolecule polymer material.
14. cavity type bioreactors according to claim 13, it is characterised in that described macromolecule polymer material is polrvinyl chloride, polypropylene, polyethylene, polymethacrylates or polyamide.
15. cavity type bioreactors according to claim 10, it is characterised in that described light heat insulation material is foam plastics.
16. cavity type bioreactors according to claim 15, it is characterised in that described foam plastics is polrvinyl chloride, polypropylene, polyethylene, polymethacrylates or polyamide.
17. cavity type bioreactors according to claim 10, it is characterized in that, described cavity wall (2) is made up of upper cavity wall (21), lower cavity wall (22) and side chamber wall (23), described side chamber wall (23) in the vertical direction, it is made up of at least two hollow sealing unit I (24), between described hollow sealing unit I (24), seals overlapping.
18. cavity type bioreactors according to claim 10, it is characterised in that the outer layer of hollow sandwich structure (3) is provided with inflation inlet (4).
19. cavity type bioreactors according to claim 17, it is characterized in that, described upper cavity wall (21) in the horizontal direction, is made up of at least two hollow sealing unit II (25), is tightly connected between described hollow sealing unit II (25).
20. cavity type bioreactors according to claim 10, it is characterised in that the bottom of described cavity (1) connects stable parts (8)；Described parts (8) of stablizing are rope, anchor or pipeline configuration；One end of described pipeline configuration connects with the inside of described cavity (1), and the other end is provided with detachable-type sealed structure (9).
21. cavity type bioreactors according to claim 10, it is characterised in that fill gas in described hollow sandwich structure (3).
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105331517A (en) * | 2015-12-08 | 2016-02-17 | 大连理工大学 | Microalgae culture system, cavity type photobioreactor and microalgae culture method |
| CN106770438A (en) * | 2016-11-21 | 2017-05-31 | 大连理工大学 | A kind of assay method of microalgae growth curve and application |
| CN107418875A (en) * | 2017-08-15 | 2017-12-01 | 大连理工大学 | Microdisk electrode shake platform and microalgae culture method |
| CN107475069A (en) * | 2017-08-15 | 2017-12-15 | 大连理工大学 | Floatation type microalgae culture system and microalgae culture method |
| CN113046229A (en) * | 2019-12-26 | 2021-06-29 | 云南爱尔发生物技术股份有限公司 | Cavity type photobioreactor in microalgae culture system |
| CN113174311A (en) * | 2021-03-16 | 2021-07-27 | 大连理工大学 | Floating photobioreactor system |
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2015
- 2015-12-08 CN CN201521013602.7U patent/CN205420364U/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105331517A (en) * | 2015-12-08 | 2016-02-17 | 大连理工大学 | Microalgae culture system, cavity type photobioreactor and microalgae culture method |
| CN106770438A (en) * | 2016-11-21 | 2017-05-31 | 大连理工大学 | A kind of assay method of microalgae growth curve and application |
| CN107418875A (en) * | 2017-08-15 | 2017-12-01 | 大连理工大学 | Microdisk electrode shake platform and microalgae culture method |
| CN107475069A (en) * | 2017-08-15 | 2017-12-15 | 大连理工大学 | Floatation type microalgae culture system and microalgae culture method |
| CN107418875B (en) * | 2017-08-15 | 2020-08-14 | 大连理工大学 | Shaking platform for microalgae culture and microalgae culture method |
| CN107475069B (en) * | 2017-08-15 | 2021-09-28 | 大连理工大学 | Floating type microalgae culture system and microalgae culture method |
| CN113046229A (en) * | 2019-12-26 | 2021-06-29 | 云南爱尔发生物技术股份有限公司 | Cavity type photobioreactor in microalgae culture system |
| CN113174311A (en) * | 2021-03-16 | 2021-07-27 | 大连理工大学 | Floating photobioreactor system |
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