CN104328031A - Surface growth type culture plate, culture unit and culture system and method - Google Patents
Surface growth type culture plate, culture unit and culture system and method Download PDFInfo
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Abstract
The invention provides a surface growth type culture plate with a membrane-coated porous plate structure. The culture plate comprises one layer of porous plate, wherein the porous plate is prepared from a rigid water absorption and water seepage material; the porous plate has a first pore diameter; each of the two sides of the porous plate is coated with one layer of ultra-micro-pore membrane; each ultra-micro-pore membrane has a second pore diameter; the second pore diameter is less than the first pore diameter; and the size of the second pore diameter of each ultra-micro-pore membrane can prevent bacteria or algae from entering and allows water and inorganic salt to seep. A culture unit comprises at least one culture plate, a solution supplying device and at least one culture solution recycling device, wherein the liquid supplying device is used for providing a culture solution to the culture plate; and the culture solution recycling device is arranged at the lower end of the culture plate. Furthermore, the invention relates to a surface growth type culture system containing the culture unit. According to the surface growth type culture plate with the membrane-coated porous plate structure, the problems of an existing immersion type microalgae culture photobioreactor system that the manufacturing and maintenance costs are expensive, the space utilization rate is low, the production efficiency is low and the energy consumption is high are solved.
Description
Technical field
The present invention relates to a kind of surface growth formula culture plate of the overlay film perforated plate construction for both culturing microalgae, cultivate unit, culture systems and method.
Background technology
Algae is that one can carry out photosynthetic aquatic planktonic algae, and micro-algae is a wherein individual little class unicellular algae to only examining under a microscope.Certain is the rich in proteins of algae own slightly, can as aquatic feed or animal and fowl fodder (as spirulina); Prior, certain slightly algae can synthesize secondary metabolite in a large number under given conditions, as grease, carotenoid, polysaccharide etc., these materials often have the biologically active substance of high economic worth, can be used in the fields such as functional food, foodstuff additive, pharmacy, bioenergy.Particularly, by micro-algae large scale culturing therefrom to extract microalgae grease, and then change into biofuel and be considered to one of most important approach solving energy shortage and carbon fixation and emission reduction.
Microdisk electrode has history decades, and current industrialization microdisk electrode is liquid submersion, using large water gaging as micro-algae life Supporting Media.Mainly comprise open cultivation pool and Closed photobioreactor (photobioreactor, PBR) two kinds of forms.The advantage of open cultivation pool is that the cost built and run is lower.But because the illuminating area/volume in open pond is smaller, fluid surface mixes poor with bottom, only have top layer frustule can accept more sufficient illumination, and pond floor cells is often difficult to receive abundant illumination; Secondly, it is more shallow that the operation depth of water is cultivated in open pond, generally only has 10-30 centimetre, and gas-liquid contact time when mending carbon that makes to ventilate is short, mends carbon efficiencies low, dissolved carbon dioxide (CO in nutrient solution
2) deficiency, photosynthesis is restricted; Another is that open intensification is slow, can not be raised to the best temperature of enzymic activity 25 DEG C at short notice, often miss the best moment that light utilizes; And temperature declines too slow naturally in Night Service formula pond, make the respiration that frustule still keeps vigorous, the energy expenditure that daytime stores is fallen, therefore makes metabolite content useful in frustule too low.Therefore the open pond cultured cells speed of growth is all lower with culture density, and it also takes up an area greatly in addition, is easily polluted.In contrast, PBR-as be adopt light transmissive material (as glass, synthetic glass, plastics film etc.) the thin thin structure made, because optical path is little, culture system illuminating area/volume ratio is comparatively large, so cell illumination is more abundant.Meanwhile, mend carbon gas and liquid contact time long, nutrient solution dissolving CO
2concentration is higher, and thus all comparatively open culture pond is high for vitro growth rates and culture density.But such PBR involves great expense usually, running cost is high, difficult in maintenance, be difficult to maximize, productive rate is 5 ~ 30g/m
2/ d, well below theoretical expectation values 100 ~ 200g/m
2/ d, do not reach the Theoretical Calculation target of industrialization, the efficiency of light energy utilization is low, and this micro-algae large scale culturing realizes the most important direct restraining factors of industrialization and still has much room for improvement.
In recent years, there is a kind of half dry type culture systems, and existing bibliographical information.But current half dry type culture systems is generally adopt filter paper, filter cloth, sponge, plastic foam, one or more in fabric (such as canvas) as substrate material.But such soft material possesses following shortcoming: (1) physical strength is low, need complicated support and the corollary apparatus such as fixing, the amplification on improper large size and spatial altitude, can only make short thin thin structure; (2) surface moisture is uneven, and micro algae growth is insecure, is easily subject to environmental influence and comes off; (3) not resistance toly to recycle, need often to change, increase cost; (4) easily drop the impurity such as fiber, and micro-algae of results is polluted; (5) water suction and water retention property poor, be unfavorable for that micro algae growth breeds the nutrient solution consumption requirement and energy cost increase increased gradually, (6) these flexible materialss are when as culture carrier, and its aspect such as bio-compatibility and chemical resistance all has certain limitation.Therefore this type of culture systems is still unsuitable for large-scale production at present.
In view of the above-mentioned shortcoming of prior art, the present invention adopts a kind of novel surface to grow formula and cultivates unit, culture systems and cultural method.
Summary of the invention
For overcoming above-mentioned shortcoming, the invention provides a kind of surface growth formula culture plate of overlay film perforated plate construction, it comprises:
One deck porous plate, this porous plate is made up of rigidity water suction seepage material, and this porous plate has the first aperture; And cover one deck ultra micro pore membrane respectively in these porous plate both sides, described ultra micro pore membrane has the second aperture, wherein the second aperture is less than the first aperture, and the size in the second aperture of described ultra micro pore membrane can prevent bacterium or algae from entering into inside, but permission water, inorganic salt ooze out.
Second aperture of described ultra micro pore membrane needs to determine according to the micro-algae kind that will cultivate, different micro-algae kinds, its particle diameter is different, second aperture of described ultra micro pore membrane need make the particle diameter being less than micro-algae, to prevent, algae kind is reverse moves to porous plate inside, and make algae kind cannot normal growth, the pollutents such as the bacterium with toxic action can also be avoided to enter to porous plate inside.Due to, the particle diameter of water molecules is about under normal circumstances
inorganic salt particle diameter is at below 2nm, and the particle diameter of micro-algae or bacterium is much larger than the particle diameter of water or inorganic salt molecule, minimum algae particle diameter is also more than 1 μm, thus there is the gap of 3 orders of magnitude between the two, then the range of choice in the second aperture of ultra micro pore membrane is also larger, water and minerals can freely ooze out in principle, and makes algae and bacterium can only rest on surface and requirement according to the invention.
In one embodiment, described porous plate is the hard porous plate be made up of substrate material and additive, and wherein, described additive comprises binding agent, and described substrate material is molecular sieve, glass sand, glass powder or green stone.
In one embodiment, described binding agent comprises aluminium stone.
In one embodiment, described binding agent also comprises sesbania powder and nitric acid.
In one embodiment, described molecular sieve has
aperture, be preferably
In one embodiment, described porous plate comprises molecular sieve and aluminium stone, and wherein said molecular sieve is 50-90 mass parts, and aluminium stone is 5-45 mass parts.
In one embodiment, described porous plate comprises molecular sieve, aluminium stone, sesbania powder and nitric acid, and wherein said molecular sieve is 60-80 mass parts, and aluminium stone is 15-35 mass parts, and sesbania powder is 1-4 mass parts, and nitric acid is 1-4 mass parts.
In one embodiment, described porous plate has first aperture of 3 ~ 40 μm.
In one embodiment, described ultra micro pore membrane formed by molecular sieve beta or molecular sieve ZSM-5.
In one embodiment, the ultra micro pore membrane covered outside described porous plate is prepared by the following method: with the preparation liquid containing molecular sieve beta or molecular sieve ZSM-5, by this porous plate at room temperature and atmospheric pressure by roller coating, spraying or immersion method process, form this ultra micro pore membrane.
In one embodiment, described ultra micro pore membrane has second aperture of 0.2 ~ 3 μm, and when this pore diameter range, inorganic salt and water molecules can permeate smoothly, and algae kind and bacterium all cannot enter into porous plate inside; More preferably, described second aperture is between 0.2 μm ~ 0.45 μm, can stop most polluted bacteria.
In one embodiment, the surface of described culture plate is roughness.
In addition, present invention also offers a kind of cultivation unit comprising above-mentioned culture plate, it comprises:
The culture plate of at least one any one scheme aforementioned, its surface is for attachment micro algae growth;
At least one liquid feed device, described liquid feed device is for providing the nutrient solution needed for micro algae growth to described culture plate, described nutrient solution is absorbed by described culture plate and infiltrates into the surface of this culture plate;
At least one nutrient solution retrieving arrangement, described culture plate lower end is located at by described nutrient solution retrieving arrangement, to be oozed out each culture plate lower end or not collected by the nutrient solution that culture plate absorbs.
In an embodiment of above-mentioned cultivation unit, described liquid feed device system covers at the top of described culture plate.
In an embodiment of above-mentioned cultivation unit, described liquid feed device is located at the top position, top of this culture plate with interval mode, and described liquid feed device provides nutrient solution to this culture plate in the mode of spray, water clock or seepage.
In an embodiment of above-mentioned cultivation unit, described cultivation unit also comprises at least one stationary installation, and this stationary installation is used for described culture plate to be fixed on a predetermined position in upright mode.
In addition, present invention also offers a kind of surface growth formula culture systems comprising above-mentioned cultivation unit, this culture systems comprises:
One by the array cultivated unit in any one embodiment above-mentioned and form, and
For providing the nutrient solution feedway of nutrient solution to the liquid feed device of the cultivation unit in described array;
Wherein, described nutrient solution feedway comprises cultivates liquid pool and cycle power device, and described cultivation liquid pool is for storing nutrient solution, and described cycle power device is used for the nutrient solution in described cultivation liquid pool to be delivered to respectively this liquid feed device.
In an embodiment of culture systems of the present invention, described cycle power device is a pump, and this pump is located at this cultivation liquid pool and is connected on the pipeline of respectively this liquid feed device.
In an embodiment of culture systems of the present invention, what described nutrient solution feedway also comprised a carbonic acid gas is mixed into device, described in be mixed into device and carbonic acid gas be mixed into in described nutrient solution cultivation pool.
In an embodiment of culture systems of the present invention, described cycle power device comprises a pressure-pot, an air compressing source of the gas, wherein this pressure-pot is connected with this cultivation pool, nutrient solution in this cultivation pool inputs in this pressure-pot temporary, this air compressing source of the gas is connected to this pressure-pot, for carrying out pressure adjustment to pressure-pot inside, this pressure-pot be connected to respectively this liquid feed device by pipeline, open this air compressing source of the gas to this pressure-pot supercharging, make the nutrient solution in pressure-pot be delivered to respectively this liquid feed device.
In an embodiment of culture systems of the present invention, this pressure-pot connects a liquidometer, the pipeline that this air compressing source of the gas is connected to this pressure-pot is provided with stopping valve, reducing valve, and the pipeline that this pressure-pot is connected to respectively this liquid feed device is provided with a tensimeter.
In an embodiment of culture systems of the present invention, this culture plate lower end is oozed out or is not collected by the nutrient solution that this culture plate absorbs and be back to cultivation liquid pool by pipeline by described nutrient solution retrieving arrangement.
In an embodiment of culture systems of the present invention, described culture plate contacts with the nutrient solution in nutrient solution retrieving arrangement.
In an embodiment of culture systems of the present invention, described cultivation unit is at least two, is provided with light supply apparatus between adjacent cultivation unit, and this light supply apparatus and described cultivation unit are arranged abreast; Or the parallel placement of described each cultivation unit forms array, and a light supply apparatus is located at the side of this array, with respectively this cultivation unit is vertical.
In an embodiment of culture systems of the present invention, described light source is natural light or source of artificial light, and described source of artificial light is two-sided light source or single surface light source.
Based on above-mentioned design, the present invention uses aforementioned surfaces growth formula culture systems to cultivate the method for micro-algae, comprises the following steps:
A) nutrient solution cultivated in liquid pool is delivered to respectively this liquid feed device by described cycle power device, described liquid feed device provides nutrient solution in the mode of spray, water clock or seepage to culture plate, thus makes the nutrient solution containing cultivating desired nutritional material infiltrate whole culture plate;
B) on described culture plate, micro-algae is inoculated;
C) ambient moisture and temperature are set, under the photon intensity of applicable micro algae growth, micro algae growth are bred.
Wherein, step a) in, also comprise and described culture plate will ooze out or be not recycled to the step of cultivating in liquid pool by the nutrient solution that culture plate absorbs, the nutrient solution composition in liquid pool is cultivated in monitoring simultaneously, supplements the nutrients in time to cultivation liquid pool.
Wherein, described photosynthetic microorganism comprises chlorella, spirulina, green alga, Isochrysis galbana, micro-plan ball algae, grid algae or blood cell algae.
In order to the surface growth formula of carrying out micro-algae is cultivated, porous plate inside above-mentioned culture plate has good water suction/permeability performance, therefore its nutrient solution absorbed can be laid in and provide supply for culture systems surface, its inner first aperture allows water molecules fully to circulate at material internal network, and due to the induced effect of water flow, nutrition small-molecule substance contained in water is made all to realize being uniformly distributed to surface by ultramicropore membrane diffusion, and micro-algae algae kind particle diameter is greater than the second aperture of ultra micro pore membrane, be not easy the inside oppositely infiltrated to porous plate, selected by the present invention, porous material has wetting ability again, its electric charge allows a large amount of micro-algae algae kind attachments (on the cliff of the growth such as similar liver moss in water).In addition, the skeletal density of this porous plate is lower, intensity and rigidity higher, relative to existing filter cloth, filter paper or canvas etc., there is following effect: (1) culture plate is certainly as rigidity, can realize in a straightforward manner self-supporting (only need simply to fixing bottom it just can uprightly), spatial altitude and size more easily realize, complicated support frame must be relied on without the need to such as canvas etc., system of can saving takes up space and cost, effective room for promotion utilising efficiency; (2) surface moisture is even, and micro algae growth is firm, is not easy be subject to environmental influence and come off; (3) tolerable environment factor impact, rotproofness is good, and repeating utilization factor is high, reduces costs; (4) not having the impurity such as fiber is mixed in the algae product of results; (5) water suction and water retention property good, be conducive to micro algae growth breeding increase gradually to nutrient solution consumption requirement.
Porous plate in the present invention and ultra micro pore membrane better be all take molecular sieve as matrix, molecular sieve is normally by TO
4the crystalline inorganic solid with microvoid structure that (T=Si, P, Al, Ge etc.) tetrahedron is formed, has different cages or cavernous structure (aperture is less than 2nm usually).Because molecular sieve can be inner to hole by the molecular adsorption less than its aperture, and the molecular repulsion larger than aperture outside its hole, play the effect of screening molecule, therefore molecular sieve of gaining the name.The practical use of zeolite molecular sieve is very widely, and such as it can be used as sorbent material, ion-exchanger, and especially can be used as petroleum cracking catalyzer, this is that people develop the power with good catalytic activity and selectivity zeolite molecular sieve.Certainly, along with people's deepening continuously to molecular sieve research, its range of application is also further expanded, and such as can be used as battery material, pharmaceutical carrier etc.These characteristics of zeolite molecular sieve depend on the feature of its mechanism and composition aspect, the multi-dimensional nature in such as duct, the size in hole, pore volume, cationic number and site, Si/Al ratio etc., can say that the performance of molecular sieve is the embodiment of the comprehensive action of this several factor.With general conventional solid adsorbent as compared with silica gel, gac, activated alumina etc., molecular sieve has two outstanding features in absorption property, and one is selective adsorption, and another is high-level efficiency absorption.
Due to the pore passage structure of component mainly wetting ability element and the multidimensional of molecular sieve, molecular sieve has good water absorption energy.Its suction type is mainly physical adsorption, and is mainly stored in the duct of molecular sieve by the water molecules adsorbed, and therefore before and after water suction, molecular screen material volume does not have considerable change.Meanwhile, molecular sieve also has fine performance in water molecules continus convergence, and this is that molecular sieve can make reason that is inner and the homogeneous humidity of surface maintenance in a long time.In addition, molecular sieve also has an important characteristic to be under the assistance of water molecules, can show good transmission performance to metal ion, and the ion of material internal is carried to surface continuously.These features determine the potentiality that molecular sieve has commercial application in semidrying microdisk electrode.
The surface growth formula culture systems water loss of overlay film perforated plate construction of the present invention is lower, microalgae cell growth is very fast, results are convenient and overall energy consumption is low, and concrete advantage is as follows:
What 1, the present invention adopted is surface growth formula microalgae culture system.After micro-algae is inoculated in the surface of ultra micro pore membrane by us, utilize liquid feed device to inject nutrient solution continuously to porous sheet material, nutrient solution reaches ultra micro pore membrane surface through porous plate inside, grow for frustule.Doing so avoids a large amount of uses of water in traditional bioreactor, decrease the energy consumption of power system; Decrease piping design; Decrease the floor space of unit yield of biomass; Meanwhile, by making micro algae growth concentration greatly improve, also simplify the techniques such as concentrated, centrifugal in results of traditional micro-algae later stage, filtration, improve production efficiency, cut operating costs;
2, in the present invention use light source to shine directly into microalgae cell, and do not need to pass through water body, therefore luminous energy decay is less, relatively traditional bioreactor, increase substantially the efficiency of light energy utilization, increased substantially micro algae growth speed and quality, this is key one step of micro-algae Biological Energy Industry simultaneously;
3, conventional tubular bioreactor needs to stir water, algae mixture, to ensure that micro-algae fully grows under the illumination condition of abundance, energy consumption and cost higher.And culture systems involved in the present invention does not need to avoid frustule sedimentation by stirring, realizing frustule and fully grow, thus decrease energy consumption, save cost;
4, relative to other bioreactors, semidrying bioreactor can change micro algae growth environment easily.Two processes of separating the growth (biomass accumulation) of micro-algae and the generation (as grease) of metabolite, because they are not identical to environmental requirement, Growth of Cells needs high nitrogen environment, and oil and fat accumulation process then needs the stressful environmental such as low nitrogen.Method conventional at present is just progressively converted into nitrogen stress induced environment when being and waiting until that in substratum system, original nitrogenous source is exhausted, and often needs more than 10 days; If growth closely plateau, thinking to enter the oil accumulation stage fast, can only be proceed in low nitrogen or nitrogen-free agar after first gathering frustule to carry out fat metabolic at present again, and this workload is very large, and energy consumption is high.And by culture systems of the present invention and cultural method, we can change growing environment at any time according to micro algae growth situation, to realize accumulation or the oil accumulation of micro algae biomass.
5, use surface growth formula culture systems of the present invention and cultural method, compared with existing surface growth formula culture systems and method, there is obvious advantage:
5a) in the present invention, its porous plate has strong water-absorbent, water-permeable, thus can for the cultivation of micro-algae, growth provide continuously moisture and other cultivate needed for nutritive ingredient.Have the inner several microporous pipelines be communicated with in porous plate, aperture is about 3 ~ 40 μm, and add that diffusion is good, described porous plate can absorb nutrient solution rapidly, and reach capacity state, carries out deposit for cultivating micro-algae on surface.When moisture evaporation reduces, can produce in duct and draw effect.Under the effect of drawing, nutrient solution can flow in the pipeline/network/hole of porous plate, nutrient solution rapid recharge can be made to ultra micro pore membrane surface, make the humidity on ultra micro pore membrane surface homogeneous saturated.By the motion of moisture, the nutritive substance small molecules in nutrient solution also can arrive Anywhere in company with water flow, and nutrient solution realizes from culture plate inner to culture plate surface translocation.Under above-mentioned natural mechanism, owing to being a spontaneous phenomenon, there is no energy consumption, thus can reduce feed flow energy consumption relative to prior art.Porous plate of the present invention and the better selection of ultra micro pore membrane are all matrix with molecular sieve, and because the specification of molecular sieve is homogeneous, the flowing that can realize liquid is quick and even;
The porous plate manufactured by molecular sieve and ultra micro pore membrane have dual porous, are describedly dual porously
1. self porousness, that is, molecular sieve itself has aperture, thus has strong absorptive and water-retentivity;
2. the porousness of manufactured Board or ultra micro pore membrane, namely, the molecular sieve porous plate made or ultra micro pore membrane also have the aperture except the aperture of molecular sieve own, this aperture is greater than the aperture of molecular sieve own, increase the contact area of hydrobiont on porous plate surface with water, and increase the mobility of water, thus be conducive to the nutrient solution being rich in nutritive ingredient to be carried to hydrobiont.The porous cooperation of the porousness of this molecular sieve and plate/ultra micro pore membrane substantially increases water-absorbent and the water-retentivity of porous plate, and adds nutrient solution is delivered to hydrobiological ability, is conducive to hydrobiont growth;
5b) culture plate of the present invention is rigidity, and its physical strength is high, in vertical direction self-supporting, only can need fix its bottom support simply, be convenient to the extension realized in size, can improve effective utilising efficiency of unit space;
5c) culture plate erosion resistance of the present invention is good, thus after harvesting or pollute after, can sterilizing agent be tolerated, thus ensure that its recycling is good; And
5d) culture plate cost of the present invention is low, the current relative maturity of molecular screen material process for machining and manufacturing, and the starting material that can select can from originally Yuing the waste residue of landfill disposal.
5e) the present invention is film covering type porous plate, wherein porous plate is made by the mode of compression moulding, first aperture of its porous plate is by regulating the sheets thus obtained pore diameter range of the adjustment such as dynamics of the grain diameter of pressed material, tackiness agent mixed volume and pressing, and the ultra micro pore membrane that porous plate outer surface covers has finer and close hole, the size in these fine and close holes is by the molecular sieve concentration of preparation liquid, the adjustment such as degree of scatter and grain diameter size, make its aperture can prevent algae kind and bacterium from entering porous plate inside, but do not affect water permeation; Each performs its own functions to make culture plate internal and external parts.
From above-mentioned advantage, this overcomes the technical bottleneck that microdisk electrode mass-producing runs into a great extent---and equipment maximizes and improves space availability ratio obstacle, makes micro-algae Biological Energy Industry have possibility.
6, culture plate of the present invention, by the porous plate of rigid material at normal temperatures and pressures, through the preparation liquid (form of similar coating) containing molecular sieve beta or molecular sieve ZSM-5 by roller coating, spraying or immersion method process, form one deck ultra micro pore membrane in the outside of porous plate.The aperture of described ultra micro pore membrane is less than the aperture of porous plate, and wherein can flow wherein and infiltrate into surface by nutrient solution easily in the aperture of porous plate; And described ultra micro pore membrane provides algae kind to adhere to, by this ultra micro pore membrane, the pollution substances such as bacterium can be reduced and enter into porous plate inside, the recycling of porous plate can not be affected; Also can play simultaneously and increase water permeation resistance, regulate the seepage discharge of culture plate surface water, make it reach the water yield of applicable algae growth, avoid nutrient solution to waste.
The extra benefit of another one is, not only can reduce the molecular sieve waste disposal cost making catalyzer, play Environmental Role, but also carried out environmental improvement at low cost.
In sum, the photo-bioreactor system manufacture and the maintenance cost that the invention solves existing immersion microdisk electrode are expensive, the problem that space availability ratio is low, production efficiency is low, energy consumption is high.In the present invention, not only culture systems is relatively cheap, and the utilising efficiency of micro-algae to luminous energy, carbon source and nutrition is high, and the formation speed of secondary metabolite is fast, significantly improves unit occupied area Biomass yield and secondary metabolite productive rate.
Accompanying drawing explanation
Fig. 1 is the schematic diagram cultivating unit.
Fig. 2 is the schematic diagram of the 1000a of surface growth formula culture systems.
Fig. 3 is the schematic diagram of surface growth formula culture systems 1000b.
Embodiment
It should be noted that the implication that in the present invention, " rigidity " refers to refers to the material relative to canvas, cloth or sponge with larger hardness, the feature of these materials all can realize self-supporting under the state of vertical direction without special bracing frame and erosion resistance is strong.
Below in conjunction with drawings and Examples, the invention will be further described.
The surface growth formula culture plate > of < overlay film perforated plate construction
The surface growth formula culture plate 1 of overlay film perforated plate construction of the present invention, be that the both sides of one deck porous plate cover one deck ultra micro pore membrane more respectively, and this porous plate is made up of the rigidity seepage material that absorbs water, this porous plate has the first aperture; Described ultra micro pore membrane has the second aperture, wherein the second aperture is less than the first aperture, the aperture of the plate had when this first aperture is and is formed by porous plate usually, and the large I in the second aperture makes water and minerals pass through smoothly, but algae grain and bacterium cannot enter infiltration ultra micro pore membrane.
Wherein, described culture plate is by following methods manufacture: will have
the substance A (as molecular sieve porous material) in aperture and adhesive substance B (as aluminium stone class auxiliary) with ratio of quality and the number of copies about 70 parts: 25 parts mixing, in the techniques well known process of room temperature and normal pressure, form the microballoon that granularity is about 50nm, then at 80-100 DEG C and normal pressure, bonding mixture is suppressed and forms hard porous plate, the better formation in its surface is as the male and fomale(M&F) of wave, and to increase its surface-area, described porous plate has first aperture of 3 ~ 40 μm.Then this porous plate is processed through following again: with the preparation liquid containing molecular sieve beta or molecular sieve ZSM-5, preparation liquid is for matrix with beta or ZSM-5 molecular sieve, take water as dispersion medium, add the coating that auxiliary agent is made, the method of making into of preparation liquid and the similar emulsion paint of form, by this porous plate at room temperature and atmospheric pressure by roller coating, spraying or immersion method process, form this ultra micro pore membrane, described ultra micro pore membrane has the second aperture of 0.2-3 μm or 0.2-2 μm, is more preferably second aperture of 0.2 μm-0.45 μm.
In general, the aperture of ultra micro pore membrane determines according to the algae kind of required cultivation, and algae kind particle diameter is less, then the second aperture of ultra micro pore membrane is also less, prevents algae kind from being entered into porous plate inside by suck-back, cannot normal growth breeding.Meanwhile, less compared to the aperture of porous plate inside in the aperture of the ultra micro pore membrane of the outer side of porous plate, make surface-moisture can not overflow and evaporation fast, reach effective water holding effect.
Wherein culture plate can also adopt following method manufacture: will have
the substance A (as molecular sieve porous material) in aperture and adhesive substance B (as aluminium stone class auxiliary) with ratio of quality and the number of copies about 70 parts: 25 parts mixing, adding addition of C (as sesbania and a small amount of additive of nitric acid) to said mixture carries out bonding, at 80-100 DEG C and normal pressure, bonding mixture is suppressed and forms hard porous plate, described porous plate has first aperture of 3 ~ 40 μm; Again through with the preparation liquid process containing molecular sieve beta or molecular sieve ZSM-5, cover the ultra micro pore membrane that one deck has the second aperture of 0.2-3 μm or 0.2-2 μm in porous plate two sides.Secondly the first pore size scope that the porous plate be pressed into has can be the amount of adhesive substance B mainly through changing the particle diameter of molecular sieve-4 A, the mode, pressure etc. of compacting realize the adjustment of the first pore size; In general, the median size of molecular sieve is larger, the amount of adhesive substance B is fewer, and pressure is less, and compression moulding porous plate first aperture obtained is larger.
Wherein, the material for the formation of porous plate can also select molecular sieve, glass sand, glass powder or green stone, and the adhesive substance used can select aluminium stone, and the mixture of aluminium stone, sesbania powder and nitric acid composition.Wherein molecular sieve has
aperture, be preferably
When described porous plate is made up of molecular sieve and aluminium stone, wherein said molecular sieve is 50-90 mass parts, and aluminium stone is 5-45 mass parts.When described porous plate is made up of molecular sieve and aluminium stone, sesbania powder and nitric acid, wherein said molecular sieve is 60-80 mass parts, and aluminium stone is 15-35 mass parts, and sesbania powder is 1-4 mass parts, and nitric acid is 1-4 mass parts.
The cultivation unit > of < culture plate composition
Fig. 1 shows cultivation unit 101 of the present invention, cultivates unit 101 and comprises culture plate 1, stationary installation 2, nutrient solution retrieving arrangement 3, liquid feed device 4.Described stationary installation 2 can comprise the assembly 21 and 22 that is relatively fixed, and forms the hole 23 can fixing culture plate 1 between fixation kit 21 and 22.Cultivate the array that unit 100 can comprise multiple culture plate 1, it forms larger plane culture plate assembly.Liquid feed device 4 is arranged on the top of culture plate 1, and liquid feed device 4 can have the outlet such as hole, gap, thus nutrient solution enters culture plate 1 with the form such as water clock, seepage.The top lateral margin of culture plate 1 is salable in liquid feed device 4.As replacement, liquid feed device 4 can be vertically spaced apart with culture plate 1, drops to culture plate 1 to make nutrient solution from liquid feed device 4.The lower end side of culture plate 1 is salable in nutrient solution retrieving arrangement 3, contacts with the nutrient solution in nutrient solution retrieving arrangement 3.
< culture systems embodiment 1>
Composition graphs 1 and Fig. 2 are described, Fig. 2 shows surface growth formula culture systems 1000a's of the present invention, surface growth formula culture systems 1000a can comprise multiple cultivation unit 101 as shown in Figure 1 and be arranged on and cultivates light supply apparatus 8 between unit 101, and provides the nutrient solution feedway 9 of nutrient solution for cultivating unit 101.
As described in Fig. 1 and 2, described stationary installation 2 comprises the assembly 21 and 22 that is relatively fixed, and forms the hole 23 can fixing culture plate 1 between fixation kit 21 and 22, and culture plate 1 can also upwards take out from hole 23.
Multiple cultivation unit 101 in Fig. 2 are set to be parallel to each other and are spaced a distance.Between every two cultivation unit 101, at least one light supply apparatus 8 is set.Light supply apparatus can be two-sided light source, irradiates the cultivation unit of both sides.Light supply apparatus 8 can be also single surface light source as required.
The surface growth formula culture systems 1000a of Fig. 2 comprises nutrient solution feedway 9, and this nutrient solution feedway 9 comprises cultivates liquid pool 6, recycle pump 7.
Nutrient solution retrieving arrangement 3 cultivates liquid pool 6 for being collected by the nutrient solution oozed out from porous plate 1 lower end or non-overlay film is absorbed by porous plate 1 and being back to by pipeline.When culture systems 1000a works, recycle pump 7 starts, by the nutrient solution cultivated in liquid pool 6 by Cemented filling to liquid feed device 4, nutrient solution is supplied culture plate 1 by liquid feed device 4, culture plate 1 is absorbed nutrient solution and is transported to the surface of culture plate 1 by the tiny aperture of its inside, be provided as the nutrition needed for micro algae growth, and culture plate 1 end contacts with the nutrient solution in nutrient solution retrieving arrangement 3 simultaneously, nutrient solution infiltrates the subregion of culture plate 1 lower end by wicking action, makes culture plate 1 be in leather hard.
Nutrient solution feedway 9 also can comprise carbonic acid gas and be mixed into device (not shown), for being mixed in nutrient solution by carbonic acid gas.
< culture systems embodiment 2>
Composition graphs 1 and Fig. 3 illustrate another culture systems 1000b of the present invention.Fig. 3 shows surface growth formula culture systems 1000b of the present invention.Similar with the embodiment shown in Fig. 2, culture systems 1000b also can comprise the cultivation unit 101 of one or more Fig. 1.Particularly, culture systems 1000b can comprise culture plate 1, stationary installation 2, nutrient solution retrieving arrangement 3, liquid feed device 4, cultivate liquid pool 6, pressure-pot 11, air compressing source of the gas 12, liquidometer 13, stopping valve 14, stopping valve 15, reducing valve 16, vent valve 17 and tensimeter 18.
The surface growth formula culture systems 1000b of Fig. 3 comprises nutrient solution circulation device 9b, optional employing air compressing mode in described nutrient solution circulation device 9b.This air compressing mode equipment therefor is this area other pressure pump replacement device optional, optional but be not limited to comprise pressure-pot 11, air compressing source of the gas 12, liquidometer 13 and mating valve, preferred stopping valve 14,15, reducing valve 16 etc.By by pressure-pot 11 lay in enough nutrient solutions condition under, valve 15 is cut in its upstream in circulation device 9b close, stopping valve 14 is opened, nutrient solution in pressure-pot 11 is made to be delivered to liquid feed device 4 in circulation device 9b by air compressing source of the gas 12, tensimeter 18 can be selected to monitor, liquid feed device 4 is with spray, the mode of water clock or seepage provides nutrient solution to culture plate 1, thus make the nutrient solution containing cultivating desired nutritional material infiltrate whole culture plate 1, nutrient solution retrieving arrangement 3 is positioned at the below of culture plate 1, do not flow to nutrient solution retrieving arrangement 3 by the nutrient solution that culture plate 1 absorbs, nutrient solution in nutrient solution retrieving arrangement 3 is flow to by back of pipeline and cultivates liquid pool 6.
the operation of hydrobiont surface growth formula culture systems
The surface growth formula culture systems 1000a of the embodiment of the present invention 1 can operate as follows:
-by nutrient solution feedway 9, liquid feed device 4 is had to comprise CO
2the nutrient solution of microvesicle, liquid feed device 4 provides nutrient solution in the mode of spray, water clock or seepage to culture plate 1;
-culture plate 1 is kept in touch with the nutrient solution in nutrient solution retrieving arrangement 3, thus make containing CO
2the nutrient solution of microvesicle infiltrates culture plate 1;
-in the micro-algae of the surface seeding of culture plate 1;
-utilize described light supply apparatus 8 to irradiate described culture plate 1, and the ambient moisture arranged around culture systems 1000a and temperature, temperature is generally 20-35 DEG C, and humidity, in the scope of 50-80%, makes micro algae growth.
The surface growth formula culture systems 1000b of the embodiment of the present invention 2 can operate as follows:
1. vent valve 17 is opened, and stopping valve 14 and stopping valve 15 are closed, and reducing valve 16 pressure is adjusted to 0MPa, and reducing valve 16 requires within the scope of 0-1.0MPa adjustable;
2. cultivating the nutrient solution adding certain volume amount in liquid pool 6;
3. open stopping valve 15, nutrient solution is from cultivation liquid pool 6 feed pressure tank 11, and liquid level is below the line entry that stopping valve 14 is connected;
4. close stopping valve 15 and vent valve 17, open stopping valve 14, the pressure of adjustment reducing valve 16, the nutrient solution in pressure-pot 11 can flow into liquid feed device 4 gradually, for culture plate 1 provide micro-algae grow required nutrient solution.Wherein, the pressure that the micro algae growth situation carried according to culture plate 1 and tensimeter 18 show, by the pressure adjusting of reducing valve 16 to suitable value;
5. the nutrient solution flowing through nutrient solution retrieving arrangement 3 turns back to be cultivated in liquid pool 6, carries out Real-Time Monitoring simultaneously, if composition does not reach fostering requirement, supplement corresponding nutrient solution composition in time to the composition cultivated in liquid pool 6;
6. be provided with the liquidometer 13 of low Level Detection in pressure-pot 11, after liquid level is lower than the liquid level set, opened by vent valve 17, stopping valve 15 is opened, and stopping valve 14 is closed, and nutrient solution is from cultivation liquid pool 6 feed pressure tank 11.The process of repeating step 3 to step 5, realizes algae non-interruptible supply nutrient solution micro-on culture plate 1;
7., after nutrient solution infiltrates whole culture plate 1, the surface of culture plate 1 is inoculated micro-algae;
8. utilize light supply apparatus 8 to irradiate culture plate 1, and the ambient moisture arranged around culture systems 100 and temperature, temperature is generally 20-35 DEG C, and humidity, in the scope of 50-80%, makes hydrobiont grow.
The system of the embodiment of the present invention 2, by tensimeter 18, reducing valve 16 and liquidometer 13, comparatively accurately can control feed rate and the speed of nutrient solution according to micro algae growth situation, realize the object precisely controlled.
Be below that cultivation unit of the present invention and prior art are compared, embodied to make this technique effect of the present invention further:
The preparation of embodiment 1 culture plate of the present invention and contrast
The preparation example of culture plate 1 of the present invention:
First, by with the A:B quality of 1:1 than compounding substances A and substance B, described substance A is molecular sieve ZSM-5, has about
aperture, mixture described in the temperature ranges of 80 to 100 DEG C and the process of normal pressure scope, cooling forming porous plate, the porous plate obtained has first aperture of about 20 μm.
Then, to the both side surface brushing preparation liquid C (by roller coating, spraying or immersion method process) of above-mentioned shaping porous plate, the quality of C is no more than 5% of above-mentioned shaping porous plate gross weight, the better material be chosen as containing molecular sieve ZSM-5 of C, under the temperature ranges of 80 to 100 DEG C and condition of normal pressure, C is formed on porous plate surface there is the ultra micro pore membrane that the second aperture is 0.9-1.1 μm.Obtain culture plate of the present invention 1..
Or, both side surface brushing preparation liquid C'(to above-mentioned shaping porous plate is coated with by roller, sprays or immersion method process), the quality of C' is no more than 5% of described mixture, the better material be chosen as containing molecular sieve beta of C', under the temperature ranges of 80 to 100 DEG C and condition of normal pressure, C' is formed on porous plate surface there is the ultra micro pore membrane that the second aperture is 1.9-2.2 μm.Obtain culture plate of the present invention 2..
Described culture plate size 1. and is 2. 1 meter * 2 meters * 3 centimetres.
Two groups of contrasts are set:
1) contrast canvas (32), size and described culture plate 1 quite, sandwich of layers is promising sponge sucks in water material.
2) without the contrast porous plate of overlay film, its preparation process is: first, by with the A:B quality of 1:1 than compounding substances A and substance B, described substance A is molecular sieve ZSM-5, has about
aperture, mixture described in the temperature ranges of 80 to 100 DEG C and the process of normal pressure scope, after cooling forming, gained porous plate is formed has first aperture of about 20 μm.The size of this contrast porous plate is suitable with described culture plate 1.
Embodiment 2 water-intake rate compares
Table 1. water-intake rate compares
From table 1, culture plate water-intake rate of the present invention is respectively apparently higher than contrast canvas and the contrast porous plate without overlay film.
Embodiment 3 culture experiment
3.1. nutrient solution composition
Consist of example with the nutrient solution that chlorella is cultivated, see the following form 2.
The nutrient solution composition that table 2 chlorella is cultivated
| Component | Usage quantity | Mother liquor |
| NaNO 3 | 1mL/L | 25g/100ml dH 2O |
| K 2HPO 4 | 1mL/L | 7.5g/100ml dH 2O |
| MgSO 4·7H 2O | 1mL/L | 7.5g/100ml dH 2O |
| CaCl 2·2H 2O | 1mL/L | 2.5g/100ml dH 2O |
| KH 2PO 4 | 1mL/L | 17.5g/100ml dH 2O |
| NaCl | 1mL/L | 2.5g/100ml dH 2O |
| Na 2CO 3 | 1mL/L | 0.2g/100ml dH 2O |
| FeCl 3·6H 2O | 1mL/L | 0.05g/100ml dH 2O |
| EDTA-Fe | 1mL/L | |
| Trace metal solutions | 1mL/L |
| Soil extract | 40mL/L |
The formula of table 3 trace metal solutions
| Component | Mother liquor |
| H 3BO 3 | 2.86g/100mL dH 2O |
| MnCl 2·4H 2O | 1.86g/100mL dH 2O |
| ZnSO 4·7H 2O | 0.22g/100mL dH 2O |
| Na 2MO 4·2H 2O | 0.39g/100mL dH 2O |
| CuSO 4·5H 2O | 0.08g/100mL dH 2O |
| Co(NO 3) 2·6H 2O | 0.05g/100mL dH 2O |
In addition, in nutrient solution, the preparation method of EDTA-Fe is: get 4.1mL concentrated hydrochloric acid distilled water diluting to 50mL, make 1N HCl; Take 0.93g EDTA-Na
2and be dissolved in 500mL distilled water, make 0.1N EDTA-Na
2solution; Take FeCl
36H
2o 0.90g is also dissolved in the above-mentioned 1N HCl of 10mL, then with 10ml 0.1N EDTA-Na
2solution mixes, and adds distilled water diluting to 1000mL.Soil extract liquid making method is: get the garden soil 200g not executing overfertilization and be placed in beaker or triangular flask, add distilled water 1000mL, bottleneck porous plug seals, boiling water heats 3 hours in a water bath, and cooling, precipitates 24 hours, this process carries out 3 times continuously, then filter, get supernatant liquor, save backup in 4 DEG C of refrigerators after sterilizing in high-pressure sterilizing pot.
According to the formula of as above table 2 and table 3, chlorella nutrient solution used is cultivated in preparation.Be stored in container stand-by.
3.2. the assembling of culture plate culture systems of the present invention and control group
According to the culture systems shown in Fig. 1-2, connect respectively and cultivate unit 101 and nutrient solution feedway 9, pipeline is connected to liquid feed device 4, nutrient solution retrieving arrangement 3 is connected to cultivates liquid pool 6, open pump 7, the nutrient solution cultivated in liquid pool 6 is delivered to each culture plate 1., in the liquid feed device 4 of culture plate 2. set by upper end, and liquid feed device 4 drips to the top lateral margin of porous plate 1 in the mode of drippage.
Again according to the culture systems shown in Fig. 1-2, what just 1. culture plate is wherein changed into same size contrasts porous plate, canvas without overlay film.
the operation of 3.3 culture systems
With reference to described in Fig. 2, surface growth formula of the present invention is cultivated unit 1000a and is used for cultivating micro-algae, can comprise the following steps:
The culture systems of the culture systems shown in-service chart 2 and control group;
-at culture plate 1., 2., contrast porous plate, canvas the micro-algae of surface seeding;
Utilize described light supply apparatus 8 to irradiate described culture plate and 1., 2., without overlay film contrast porous plate, canvas, and the envrionment temperature arranged around culture systems 1000a is generally 20-35 DEG C, humidity, in the scope of 50-80%, makes micro algae growth.
4. culture Comparative result
After above-mentioned culture systems cultivates 24,48 and 72 hours, respectively from the culture plate of the present invention of same homalographic 1. and 2., contrast canvas, contrast harvesting microalgae culture porous plate without overlay film, to weigh algae mud, after 70 DEG C of drying and dehydratings, take dry weight and carry out dry biomass weight ratio comparatively.
Implement the results contrast of the surface growth formula culture systems of the embodiment of the present invention 1:
From table 4, micro-algae dry biomass is all significantly higher than contrast canvas in the output of culture plate 1 of the present invention, contrasts porous plate apparently higher than without overlay film.And the slightly large culture plate in the aperture of ultra micro pore membrane 2., 1. its dry biomass heavily exceedes culture plate.
Claims (28)
1. a surface growth formula culture plate for overlay film perforated plate construction, it is characterized in that, this culture plate comprises:
One deck porous plate, this porous plate is made up of rigidity water suction seepage material, and this porous plate has the first aperture; And cover one deck ultra micro pore membrane respectively in these porous plate both sides, described ultra micro pore membrane has the second aperture, wherein the second aperture is less than the first aperture, and the size in the second aperture of described ultra micro pore membrane can prevent bacterium or algae from entering into inside, but allows water and minerals to ooze out.
2. the surface growth formula culture plate of overlay film perforated plate construction according to claim 1, it is characterized in that, described porous plate is the hard porous plate be made up of substrate material and additive, wherein, described additive comprises binding agent, and described substrate material is molecular sieve, glass sand, glass powder or green stone.
3. the surface growth formula culture plate of overlay film perforated plate construction according to claim 2, it is characterized in that, described binding agent comprises aluminium stone.
4. the surface growth formula culture plate of overlay film perforated plate construction according to claim 3, it is characterized in that, described binding agent also comprises sesbania powder and nitric acid.
5. the surface growth formula culture plate of overlay film perforated plate construction according to claim 2, it is characterized in that, described molecular sieve has
aperture.
6. the surface growth formula culture plate of overlay film perforated plate construction according to claim 2, is characterized in that, described porous plate comprises molecular sieve and aluminium stone, and wherein said molecular sieve is 50-90 mass parts, and aluminium stone is 5-45 mass parts.
7. overlay film perforated plate construction according to claim 4 surface growth formula culture plate, it is characterized in that, described porous plate comprises molecular sieve, aluminium stone, sesbania powder and nitric acid, wherein said molecular sieve is 60-80 mass parts, aluminium stone is 15-35 mass parts, sesbania powder is 1-4 mass parts, and nitric acid is 1-4 mass parts.
8. the surface growth formula culture plate of the overlay film perforated plate construction according to Claims 2 or 3 or 4, is characterized in that, described porous plate has first aperture of 3 ~ 40 μm.
9. the surface growth formula culture plate of the overlay film perforated plate construction according to Claims 2 or 3 or 4, is characterized in that, described ultra micro pore membrane formed by molecular sieve beta or molecular sieve ZSM-5.
10. the surface growth formula culture plate of overlay film perforated plate construction according to claim 9, it is characterized in that, the ultra micro pore membrane covered outside described porous plate obtains by the following method: with the preparation liquid containing molecular sieve beta or molecular sieve ZSM-5, by this porous plate at room temperature and atmospheric pressure by roller coating, spraying or immersion method process, form this ultra micro pore membrane.
The surface growth formula culture plate of 11. overlay film perforated plate constructions as described in claim 9 or 10, it is characterized in that, described ultra micro pore membrane has second aperture of 0.2 ~ 3 μm.
The surface growth formula culture plate of 12. overlay film perforated plate constructions as claimed in claim 1, it is characterized in that, the surface of described culture plate is roughness.
13. 1 kinds of surface growth formulas cultivate unit, and its feature comprises:
At least one culture plate as described in claim 1-12, its surface is for attachment micro algae growth;
At least one liquid feed device, described liquid feed device is for providing the nutrient solution needed for micro algae growth to described culture plate, described nutrient solution is absorbed by described culture plate and infiltrates into the surface of this culture plate;
At least one nutrient solution retrieving arrangement, described culture plate lower end is located at by described nutrient solution retrieving arrangement, to be oozed out each culture plate lower end or not collected by the nutrient solution that culture plate absorbs.
14. surface growth formulas according to claim 13 cultivate unit, and it is characterized in that, described liquid feed device system covers at the top of described culture plate.
15. surface growth formulas according to claim 13 cultivate unit, it is characterized in that, described liquid feed device is located at the top position, top of this culture plate with interval mode, and described liquid feed device provides nutrient solution to this culture plate in the mode of spray, water clock or seepage.
16. surface growth formulas according to claim 13 cultivate unit, and it is characterized in that, described cultivation unit also comprises at least one stationary installation, and this stationary installation is used for described culture plate to be fixed on a predetermined position in upright mode.
17. 1 kinds of surface growth formula culture systems, this culture systems comprises:
An array be made up of the cultivation unit described in any one of claim 13-16, and
For providing the nutrient solution feedway of nutrient solution to the liquid feed device of the cultivation unit in described array;
Wherein, described nutrient solution feedway comprises cultivates liquid pool and cycle power device, and described cultivation liquid pool is for storing nutrient solution, and described cycle power device is used for the nutrient solution in described cultivation liquid pool to be delivered to respectively this liquid feed device.
18. surface growth formula culture systems according to claim 17, is characterized in that, described cycle power device is a pump, and this pump is located at this cultivation liquid pool and is connected on the pipeline of respectively this liquid feed device.
19. surface growth formula culture systems according to claim 17 or 18, is characterized in that, what described nutrient solution feedway also comprised a carbonic acid gas is mixed into device, described in be mixed into device and carbonic acid gas be mixed into in described nutrient solution cultivation pool.
20. surface growth formula culture systems according to claim 17, it is characterized in that, described cycle power device comprises a pressure-pot, an air compressing source of the gas, wherein this pressure-pot is connected with this cultivation pool, nutrient solution in this cultivation pool inputs in this pressure-pot temporary, this air compressing source of the gas is connected to this pressure-pot, for carrying out pressure adjustment to pressure-pot inside, this pressure-pot be connected to respectively this liquid feed device by pipeline, open this air compressing source of the gas to this pressure-pot supercharging, make the nutrient solution in pressure-pot be delivered to respectively this liquid feed device.
21. surface growth formula culture systems according to claim 20, it is characterized in that, this pressure-pot connects a liquidometer, and the pipeline that this air compressing source of the gas is connected to this pressure-pot is provided with stopping valve, reducing valve, and the pipeline that this pressure-pot is connected to respectively this liquid feed device is provided with a tensimeter.
22. surface growth formula culture systems according to claim 17 or 18 or 20, is characterized in that, this culture plate lower end is oozed out or do not collected by the nutrient solution that this culture plate absorbs and be back to cultivation liquid pool by pipeline by described nutrient solution retrieving arrangement.
23. surface growth formula culture systems according to claim 22, it is characterized in that, described culture plate contacts with the nutrient solution in nutrient solution retrieving arrangement, makes the nutrient solution in nutrient solution retrieving arrangement be infiltrated the subregion of the bottom of this culture plate by wicking action.
24. surface growth formula culture systems according to claim 17 or 18 or 20, it is characterized in that, described cultivation unit is at least two, is provided with light supply apparatus between adjacent cultivation unit, and this light supply apparatus and described cultivation unit are arranged abreast; Or the parallel placement of described each cultivation unit forms array, and a light supply apparatus is located at the side of this array, with respectively this cultivation unit is vertical.
25. surface growth formula culture systems according to claim 17 or 18 or 20, it is characterized in that, described light source is natural light or source of artificial light, and described source of artificial light is two-sided light source or single surface light source.
26. 1 kinds of methods using the surface growth formula culture systems described in any one of claim 17-25 to cultivate micro-algae, comprise the following steps:
A) nutrient solution cultivated in liquid pool is delivered to respectively this liquid feed device by described cycle power device, described liquid feed device provides nutrient solution in the mode of spray, water clock or seepage to culture plate, thus makes the nutrient solution containing cultivating desired nutritional material infiltrate whole culture plate;
B) on described culture plate, micro-algae is inoculated;
C) ambient moisture and temperature are set, under the photon intensity of applicable micro algae growth, micro algae growth are bred.
The method of the micro-algae of 27. cultivation according to claim 26, it is characterized in that, step a) in, also comprise and will described culture plate ooze out or be not recycled to the step of cultivating in liquid pool by the nutrient solution that culture plate absorbs, the nutrient solution composition in liquid pool is cultivated in monitoring simultaneously, supplements the nutrients in time to cultivation liquid pool.
The method of the micro-algae of 28. cultivation according to claim 26, is characterized in that, described micro-algae algae kind comprises chlorella, spirulina, green alga, grid algae, Isochrysis galbana, micro-plan ball algae or blood cell algae.
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