CN104328029A - Surface growth type photosynthetic microorganism culture plate having core-shell structure, culture unit, system and culture method - Google Patents
Surface growth type photosynthetic microorganism culture plate having core-shell structure, culture unit, system and culture method Download PDFInfo
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- CN104328029A CN104328029A CN201410599353.8A CN201410599353A CN104328029A CN 104328029 A CN104328029 A CN 104328029A CN 201410599353 A CN201410599353 A CN 201410599353A CN 104328029 A CN104328029 A CN 104328029A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M31/00—Means for providing, directing, scattering or concentrating light
- C12M31/10—Means for providing, directing, scattering or concentrating light by light emitting elements located inside the reactor, e.g. LED or OLED
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
Abstract
The invention discloses a surface growth type photosynthetic microorganism culture plate having a core-shell structure, which comprises a shell plate arranged outside and a core part arranged in the shell plate, wherein the shell plate is of a porous plate-shaped structure made from a rigid water seepage material; the filling core is made from a water retention and water seepage material; and the filling core is arranged in the shell plate. According to the core-shell type composite culture plate disclosed by the invention, photosynthetic microorganisms are attached to the surface, a nutrient solution for supporting growth of the photosynthetic microorganisms is retained in the filling core, and the nutrient solution gradually permeates to the surface of the shell plate from the filling core and then is supplied for sustainable reproduction of the photosynthetic microorganisms. In addition, the invention further discloses a culture unit comprising the core-shell type composite culture plate, a culture system, a culture method and application thereof.
Description
Technical field
The surface growth formula photosynthetic microorganism culture plate that the present invention relates to nucleocapsid structure and the cultivation unit comprising this culture plate, surface growth formula culture systems, method and application thereof.
Background technology
Photosynthetic microorganism is a class is unique with light or the microorganism of main energy sources and growth and breeding, and comprise micro-algae, cyanobacteria etc. and can carry out photosynthetic microorganism containing chlorophyll, microorganism has important biomolecule utility value.According to current bibliographical information, algae rich in proteins, can as aquatic feed or animal and fowl fodder (as spirulina); Prior, certain slightly algae can synthesize secondary metabolite in a large number under given conditions, as grease, carotenoid, polysaccharide etc., these materials have the biologically active substance of high economic worth often, can be used in the fields such as functional food, foodstuff additive, pharmacy, bioenergy.Particularly extract microalgae grease by micro-algae large scale culturing, and then conversion production biofuel is considered to one of most important approach solving bioenergy production and carbon fixation and emission reduction.
Microdisk electrode has history decades, and current industrialization microdisk electrode is liquid submersion, using large water gaging as micro-algae life Supporting Media.Mainly comprise open cultivation pool and Closed photobioreactor (photobioreactor, PBR) two kinds of forms.The advantage of open cultivation pool is that the cost built and run is lower.But because the illuminating area/volume in open pond is smaller, fluid surface mixes poor with bottom, only have top layer frustule can accept more sufficient illumination, and pond floor cells is often difficult to receive abundant illumination; Secondly, it is more shallow that the operation depth of water is cultivated in open pond, generally only has 10-30 centimetre, and gas-liquid contact time when mending carbon that makes to ventilate is short, mends carbon efficiencies low, dissolved carbon dioxide (CO in nutrient solution
2) deficiency, photosynthesis is restricted; Another is that open intensification is slow, can not be raised to the best temperature of enzymic activity 25 DEG C at short notice, often miss the best moment that light utilizes; And temperature declines too slow naturally in Night Service formula pond, make the respiration that frustule still keeps vigorous, the energy expenditure that daytime stores is fallen, therefore makes metabolite content useful in frustule too low.Therefore the open pond cultured cells speed of growth is all lower with culturing cell density, and it also takes up an area greatly in addition.In contrast, PBR is generally the thin thin structure adopting light transmissive material (as glass, synthetic glass, plastics film etc.) to make, because optical path is little, culture system illuminating area/volume ratio is comparatively large, so cell illumination is more abundant.Meanwhile, mend carbon gas and liquid contact time long, nutrient solution dissolving (X) 2 concentration are higher, and thus all comparatively open culture pond is high for vitro growth rates and culture density.But such PBR involves great expense usually, running cost is high, difficult in maintenance, be difficult to maximize, productive rate is at 5 ~ 30g/m
2/ d, well below theoretical expectation values 100 ~ 200g/m
2/ d, does not reach the Theoretical Calculation target of industrialization, and low this of the efficiency of light energy utilization makes micro-algae large scale culturing realize the most important direct restraining factors of industrialization still to have much room for improvement.
In recent years, there is a kind of half dry type culture systems, existing patent report, all adopt one or more in filter paper, filter cloth, sponge, porous plastics, fabric (such as canvas) as substrate material, but such soft material possesses following shortcoming: 1) physical strength is low, need complicated support, amplification on improper large size and spatial altitude, short thin thin structure can only be made, 2) surface moisture is uneven, and micro algae growth is insecure; And 3) not resistance toly to recycle, need often to change, increase cost, 4) easily drop the impurity such as fiber; In addition, 5) these flexible materialss are when as culture plate surfacing, and its aspect such as bio-compatibility and chemical resistance all has certain limitation.6) when adopting these materials as surface growth formula bioreactor, owing to there is difference of altitude at vertical direction, make the hydrostaticpressure near below comparatively large, seep water very fast and produce ponding, making micro-algae algae kind be easy to be rinsed (cannot adhere to equably and grow).Therefore this type of culture systems does not enter the possibility of huge construction program production at present.
In view of the above-mentioned shortcoming of prior art, the present invention intends adopting a kind of surface growth formula photosynthetic microorganism culture plate of novel nucleocapsid structure, culture systems and cultural method.
Summary of the invention
For overcoming above-mentioned shortcoming, the invention provides a kind of surface growth formula photosynthetic microorganism culture plate of nucleocapsid structure, it comprises:
Be located at outside coverboard, this coverboard of this coverboard is the many micropores platy structure be made up of rigid porous material;
Filled core, be filled in this coverboard inner, described filled core is water conservation water-permeable material.
According to such scheme, described coverboard is a kind ofly have the network-like micro-channel structure be communicated with from the inside to the outside, and this special construction enables this coverboard absorb water, seep water, and produces natural syphonic effect; Reduce the energy consumption that nutrient solution medium transmits.
In one embodiment, in described coverboard, form at least one conduit, in described conduit, fill described filled core.
In one embodiment, described conduit comprises multiple, parallel and longitudinally run through upper and lower two end faces of described coverboard, has a floor between every two conduits, by forming region between two conduits every.
In one embodiment, described floor is one-body molded by this coverboard, or is, after other fluid-tight materials make, be installed in this coverboard.
In one embodiment, described coverboard is chosen as one or more in following material: permeable ceramic plate cement plate, potter's clay fire plate, blue bricks plate, common brick plate and watt material plate.
In one embodiment, described filled core is chosen as one or more in following material: molecular sieve, non-woven fabrics, highly dense sponge, cotton, hydroscopic high-molecular resin and urethane water-retaining agent.
In one embodiment, the form of described filled core is strip, bulk, Powdered, particulate state, cotton-shaped or shred.
In one embodiment, the horizontal section of described conduit is circle, trilateral, rectangle or ellipse.
In one embodiment, described conduit is along the vertical section perpendicular to the surface, front and back two of described coverboard, be from top to bottom run-in index, or gradually from top to bottom reduce or step type reduces, conduit inwall is from top to bottom increased gradually apart from the both side surface of this coverboard.
In one embodiment, described molecular sieve has
aperture.
In one embodiment, described molecular sieve is beta molecular sieve or ZSM-5 molecular sieve.
In one embodiment, the surface of described coverboard is roughness.
In one embodiment, described each coverboard can build folded splicing and combining, and described each coverboard inside is provided with the designated lane directly passed through for liquid.
In addition, present invention also offers a kind of cultivation unit comprising the surface growth formula photosynthetic microorganism culture plate of above-mentioned nucleocapsid structure, it comprises:
The surface growth formula photosynthetic microorganism culture plate of the nucleocapsid structure of at least one any one scheme aforementioned, its surface is for photosynthetic microorganism apposition growth;
At least one liquid feed device, described liquid feed device is for providing the nutrient solution needed for photosynthetic microorganism growth to the surface growth formula photosynthetic microorganism culture plate of described nucleocapsid structure, described nutrient solution is absorbed by the surface growth formula photosynthetic microorganism culture plate of described nucleocapsid structure and infiltrates into the surface of the surface growth formula photosynthetic microorganism culture plate of this nucleocapsid structure;
At least one nutrient solution retrieving arrangement, the surface growth formula photosynthetic microorganism culture plate lower end of described nucleocapsid structure is located at by described nutrient solution retrieving arrangement, to be oozed out the surface growth formula photosynthetic microorganism culture plate lower end of each nucleocapsid structure or not collected by the nutrient solution that the surface growth formula photosynthetic microorganism culture plate of nucleocapsid structure absorbs.
In an embodiment of above-mentioned cultivation unit, described liquid feed device system covers at the top of the surface growth formula photosynthetic microorganism culture plate of described nucleocapsid structure.
In an embodiment of above-mentioned cultivation unit, described liquid feed device is located at the top position, top of the surface growth formula photosynthetic microorganism culture plate of this nucleocapsid structure with interval mode, described liquid feed device provides nutrient solution to the surface growth formula photosynthetic microorganism culture plate of this nucleocapsid structure in the mode of spray, water clock or seepage.
In an embodiment of above-mentioned cultivation unit, described cultivation unit also comprises at least one stationary installation, and this stationary installation is used for the surface growth formula photosynthetic microorganism culture plate of described nucleocapsid structure to be fixed on a predetermined position in upright mode.
In addition, present invention also offers a kind of surface growth formula culture systems comprising above-mentioned cultivation unit, this culture systems comprises:
One by the array cultivated unit in any one embodiment above-mentioned and form, and
For providing the nutrient solution feedway of nutrient solution to the liquid feed device of the cultivation unit in described array;
Wherein, described nutrient solution feedway comprises cultivates liquid pool and cycle power device, and described cultivation liquid pool is for storing nutrient solution, and described cycle power device is used for the nutrient solution in described cultivation liquid pool to be delivered to respectively this liquid feed device.
In an embodiment of culture systems of the present invention, described cycle power device is a pump, and this pump is located at this cultivation liquid pool and is connected on the pipeline of respectively this liquid feed device.
In an embodiment of culture systems of the present invention, what described nutrient solution feedway also comprised a carbonic acid gas is mixed into device, described in be mixed into device just carbonic acid gas be mixed into in described nutrient solution cultivation pool.
In an embodiment of culture systems of the present invention, described cycle power device comprises a pressure-pot, an air compressing source of the gas, wherein this pressure-pot is connected with this cultivation pool, nutrient solution in this cultivation pool inputs in this pressure-pot temporary, this air compressing source of the gas is connected to this pressure-pot, for realizing regulating to pressure-pot internal pressure, this pressure-pot be connected to respectively this liquid feed device by pipeline, open this air compressing source of the gas to this pressure-pot supercharging, make the nutrient solution in pressure-pot be delivered to respectively this liquid feed device.
In an embodiment of culture systems of the present invention, this pressure-pot connects a liquidometer, the pipeline that this air compressing source of the gas is connected to this pressure-pot is provided with stopping valve, reducing valve, and the pipeline that this pressure-pot is connected to respectively this liquid feed device is provided with a tensimeter.
In an embodiment of culture systems of the present invention, the surface growth formula photosynthetic microorganism culture plate lower end of this nucleocapsid structure is oozed out or is not collected by the nutrient solution that the surface growth formula photosynthetic microorganism culture plate of this nucleocapsid structure absorbs and be back to cultivation liquid pool by pipeline by described nutrient solution retrieving arrangement.
In an embodiment of culture systems of the present invention, the surface growth formula photosynthetic microorganism culture plate of described nucleocapsid structure contacts with the nutrient solution in nutrient solution retrieving arrangement.
In an embodiment of culture systems of the present invention, described cultivation unit is at least two, is provided with light supply apparatus between adjacent cultivation unit, and this light supply apparatus and described cultivation unit are arranged abreast; Or the parallel placement of described each cultivation unit forms array, and a light supply apparatus is located at the side of this array, with respectively this cultivation unit is vertical.
In an embodiment of culture systems of the present invention, described light source is natural light or source of artificial light, and described source of artificial light is two-sided light source or single surface light source.
Based on above-mentioned design, the present invention uses aforementioned surfaces to grow the method for formula culture systems cultivation photosynthetic microorganism, comprises the following steps:
A) nutrient solution cultivated in liquid pool is delivered to respectively this liquid feed device by described cycle power device, described liquid feed device provides nutrient solution in the mode of spray, water clock or seepage to the surface growth formula photosynthetic microorganism culture plate of nucleocapsid structure, thus makes the surface growth formula photosynthetic microorganism culture plate infiltrating whole nucleocapsid structure containing the nutrient solution cultivating desired nutritional material;
B) on the surface growth formula photosynthetic microorganism culture plate of described nucleocapsid structure, photosynthetic microorganism is inoculated;
C) ambient moisture and temperature are set, under the photon intensity that applicable photosynthetic microorganism grows, make photosynthetic microorganism carry out photosynthesis, growth and breeding.
Wherein, step a) in, also comprise and the surface growth formula photosynthetic microorganism culture plate by described nucleocapsid structure oozes out or is not recycled to the step of cultivating in liquid pool by the nutrient solution that the surface growth formula photosynthetic microorganism culture plate of nucleocapsid structure absorbs, the nutrient solution composition in liquid pool is cultivated in monitoring simultaneously, supplements the nutrients in time to cultivation liquid pool.
Wherein, described photosynthetic microorganism comprises chlorella, spirulina, green alga, Isochrysis galbana, micro-plan ball algae, grid algae or blood cell algae.
In order to the surface growth formula of carrying out photosynthetic microorganism is cultivated, the present invention adopts the surface growth formula photosynthetic microorganism culture plate of new type structure of hud, it comprises the coverboard having water suction water permeability material and make, with the filled core there is water absorbing and retaining properties material making, this coverboard is microporous materials plate, there is good water suction/water permeability, therefore the nutrient solution of filling core and holding can be drawn, when coverboard surface is due to moisture transpiration, form inside and outside hydraulic pressure difference, through syphonic effect, the nutrient solution in filled core can be absorbed rapidly, and fill the nutrient solution that in core, liquid can absorb liquid feed device and supply rapidly, thus constantly nutrient solution is supplied to the surface of this coverboard, reach capacity state.
In addition, wherein coverboard inside forms several conduit running through the upper and lower two ends of this coverboard, and these conduits are parallel and extend along the upper and lower two ends of coverboard, has a floor between every two conduits, by forming region between two conduits every.The existence of these floors, filled core is divided into several, the hydrostatic pressure reduction brought in continuous print water route is produced to avoid a monoblock filled core inside, reduce the too fast equivalance water problems of coverboard lower external sweat, allow a large amount of photosynthetic microorganisms, as the attachment of algae kind (on the cliff of the growth such as similar liver moss in water), the present invention can solve that the infiltration speed of culture plate in the vertical direction is different, reactor surface ponding washes away the technical problems such as algae kind.
In order to solve the problem in vertical direction hydrostaticpressure further, wherein respectively this conduit can to reduce or step type reduces in gradual along perpendicular to the section on the direction of two sides before and after this coverboard, distance between making outside the arrival of the conduit inwall near this coverboard lower end coverboard increases gradually, the coverboard lower surface infiltration come with the hydrostaticpressure difference band of offsetting part is fast, micro-algae is washed away the problem come off.
Another is at effect, the surface growth formula photosynthetic microorganism culture plate of nucleocapsid structure of the present invention, the form of the filled core of its inside can be unrestricted, because this coverboard has defined one by one independently conduit, therefore this filled core can be Powdered, cotton-shaped, the form such as strip, bulk, particulate state, and filled core use for a long time aging after, the water absorbing and retaining properties filled core that can more renew easily, avoids the problem such as aging release toxin or lock water poor performance; Therefore, its maintenance cost of surface growth formula photosynthetic microorganism culture plate of nucleocapsid structure of the present invention is low.
In addition, frame strength and the rigidity of the surface growth formula photosynthetic microorganism culture plate of nucleocapsid structure of the present invention are higher, relative to existing filter cloth, filter paper or canvas etc., there is following effect: the surface growth formula photosynthetic microorganism culture plate of (1) nucleocapsid structure is certainly as rigidity, can realize in a straightforward manner self-supporting (only need simply to fixing bottom it just can be upright, or adopt lifting or mount), spatial altitude and size more easily realize, must rely on complicated support frame without the need to such as canvas etc., system of can saving takes up space and cost; (2) coverboard surface moisture is even, and micro algae growth is firm, is not easy be subject to environmental influence and come off; (3) coverboard tolerable environment factor impact, rotproofness is good, and repeating utilization factor is high, reduces costs; (4) not having the impurity such as fiber is mixed in the algae product of results; (5) water suction and water retention property good, be conducive to micro algae growth breeding increase gradually to nutrient solution consumption requirement.Coverboard structure of the present invention is better is also be matrix with molecular sieve, and molecular sieve is normally by TO
4the crystalline inorganic solid with microvoid structure that (T=Si, P, Al, Ge etc.) tetrahedron is formed, has different cages or cavernous structure (aperture is less than 2nm usually).Because molecular sieve can be inner to hole by the molecular adsorption less than its aperture, and the molecular repulsion larger than aperture outside its hole, play the effect of screening molecule, therefore molecular sieve of gaining the name.The practical use of zeolite molecular sieve is very widely, and such as it can be used as sorbent material, ion-exchanger, and especially can be used as petroleum cracking catalyzer, this is that people develop the power with good catalytic activity and selectivity zeolite molecular sieve.Certainly, along with people's deepening continuously to molecular sieve research, its range of application is also further expanded, and such as can be used as battery material, pharmaceutical carrier etc.These characteristics of zeolite molecular sieve depend on the feature of its mechanism and composition aspect, the multi-dimensional nature in such as duct, the size in hole, pore volume, cationic number and site, Si/Al ratio etc., can say that the performance of molecular sieve is the embodiment of the comprehensive action of this several factor.With general conventional solid adsorbent as compared with silica gel, gac, activated alumina etc., molecular sieve has two outstanding features in absorption property, and one is selective adsorption, and another is high-level efficiency absorption.Due to the pore passage structure of component mainly wetting ability element and the multidimensional of molecular sieve, molecular sieve has good water absorption energy.Its suction type is mainly physical adsorption, and is mainly stored in the duct of molecular sieve by the water molecules adsorbed, and therefore before and after water suction, molecular screen material volume does not have considerable change.Meanwhile, molecular sieve also has fine performance in water molecules continus convergence, and this is that molecular sieve can make reason that is inner and the homogeneous humidity of surface maintenance in a long time.In addition, molecular sieve also has an important characteristic to be under the assistance of water molecules, can show good transmission performance to metal ion, and the ion of material internal is carried to surface continuously.These features determine the potentiality that molecular sieve has commercial application in semidrying microdisk electrode.Surface growth formula culture systems water loss provided by the invention is lower, microalgae cell growth is very fast, results are convenient and overall energy consumption is low, concrete advantage is as follows: what the present invention adopted is surface growth formula culture systems, after micro-algae algae kind can being inoculated in the surface of coverboard, nutrient solution feedway is utilized to inject nutrient solution continuously in the conduit on coverboard top, nutrient solution is filled wicking harvesting storage, after filled core absorbs the state that reaches capacity, the nutrient solution supplemented again will slowly ooze out from filled core, and the surface of coverboard is oozed out into through the coverboard of microporosity matter, make the surface of coverboard all the time in moisture state, environment needed for micro algae growth and nutrition can be provided.Doing so avoids a large amount of uses of water in traditional bioreactor, decrease the energy consumption of power system; Decrease piping design; Culture plate can erect arrangement in array-like, decreases the floor space of unit yield of biomass; Meanwhile, by making micro algae growth concentration greatly improve, also simplify the techniques such as dehydration in traditional micro-algae later stage results is concentrated, centrifugal, filtration, improve production efficiency, cut operating costs;
2, in the present invention use light source to shine directly into microalgae cell, and do not need to pass through water body, therefore luminous energy decay is less, relatively traditional bioreactor, increase substantially the efficiency of light energy utilization, increased substantially micro algae growth speed and quality, this is key one step of micro-algae Biological Energy Industry simultaneously;
3, conventional tubular bioreactor needs to stir water, algae mixture, to ensure that micro-algae fully grows under the illumination condition of abundance, energy consumption and cost higher.And culture systems involved in the present invention does not need to avoid frustule sedimentation by stirring, reducing energy consumption, cost-saving;
4, relative to other bioreactors, the culture plate of surface growth formula nucleocapsid structure of the present invention can change micro algae growth environment easily.Two processes of separating the growth (biomass accumulation) of micro-algae and the generation (as grease) of metabolite, because they are not identical to environmental requirement, Growth of Cells needs high nitrogen environment, and oil and fat accumulation process then needs the stressful environmental such as low nitrogen.Method conventional at present is just progressively converted into nitrogen stress induced environment when being and waiting until that in substratum system, original nitrogenous source is exhausted, and often needs more than 10 days; If growth closely plateau, thinking to enter the oil accumulation stage fast, can only be proceed in low nitrogen or nitrogen-free agar after first gathering frustule to carry out fat metabolic at present again, and this workload is very large, and energy consumption is high.And by culture systems of the present invention and cultural method, we can change growing environment at any time according to micro algae growth situation, to realize accumulation or the oil accumulation of micro algae biomass.
5, use surface growth formula culture systems of the present invention and cultural method, compared with existing surface growth formula culture systems and method, there is obvious advantage:
5a) coverboard of the present invention has strong water suction water-permeable, filled core has strong water-absorbent, lock water-based energy, do not need to use the water delivery of pump high-power ground, trickle continuously, just can keep enough moisture, thus can for the cultivation of micro-algae, growth provide continuously moisture and other cultivate needed for nutritive ingredient, save energy consumption.The microporosity that coverboard in the present invention has, add that diffusion is good, described coverboard can absorb nutrient solution rapidly, and reach capacity state, carries out deposit for cultivating micro-algae on surface.When moisture evaporates, due to the surface tension effects of fluid surface in culture systems surface void, can produce in aperture and draw effect.Under the effect of drawing, nutrient solution can flow in pipeline/network/hole, the hole of coverboard, nutrient solution rapid recharge can be made to coverboard surface, make the humidity on coverboard surface homogeneous saturated.By the motion of moisture, the nutritive substance small molecules in molten nutrient solution also can arrive Anywhere in company with water flow, and nutrient solution realizes from culture plate inner to culture plate surface translocation.Under above-mentioned natural mechanism, owing to being a spontaneous phenomenon, thus can reduce feed flow energy consumption relative to prior art.
The better selection of coverboard material of the culture plate of nucleocapsid structure of the present invention take molecular sieve as matrix, and because the specification of molecular sieve is homogeneous, the flowing that can realize liquid is quick and even.
The coverboard manufactured by molecular sieve has dual microporosity, and described dual microporosity is 1. molecular sieve microporosity, that is, molecular sieve itself has aperture, thus has strong absorptive and water-retentivity; 2. plate microporosity, there is the network-like pipeline be communicated with from the inside to the outside, the microporosity coverboard of the molecular sieve namely made also has the aperture except the aperture of molecular sieve own, this aperture is greater than the aperture of molecular sieve own, increase the contact area of hydrobiont (as micro-algae) on coverboard surface with water, and be increased in the mobility of coverboard surface water, thus be conducive to the nutrient solution being rich in nutritive ingredient to be carried to hydrobiont.The cooperation of the microporosity of this molecular sieve and the microporosity of coverboard substantially increases water-absorbent and the water-retentivity of coverboard, and adds nutrient solution is delivered to hydrobiological ability, is conducive to hydrobiont growth;
5b) coverboard of the present invention is rigidity, and its physical strength is high, in vertical direction self-supporting, only can need fix its bottom support simply, be convenient to the extension realized in size;
5c) coverboard erosion resistance of the present invention is good, thus after harvesting or pollute after, can sterilizing agent be tolerated, thus ensure that its recycling is good; And
5d) coverboard cost of the present invention is low, the current relative maturity of molecular screen material process for machining and manufacturing, and the starting material that can select can from originally Yuing the waste residue of landfill disposal.
5e) coverboard inside of the present invention forms several conduit, holds filled core respectively, a monoblock filled core can be avoided easily to form continuous water route and there is the hydrostaticpressure problem of short transverse, avoids producing near coverboard bottom the too fast and ponding problem that seeps water; In addition due to shaping conduit, then the filled core can being convenient to any form fills, and according to water conservation situation, can substitute new filled core material.
From above-mentioned advantage, this overcomes the technical bottleneck that microdisk electrode mass-producing runs into a great extent---and equipment maximizes and improves space availability ratio obstacle, makes micro-algae Biological Energy Industry have possibility.
The extra benefit of another one is, not only can reduce the molecular sieve waste disposal cost making catalyzer, play Environmental Role, but also carried out environmental improvement at low cost.
In sum, the photo-bioreactor system manufacture and the maintenance cost that the invention solves existing immersion microdisk electrode are expensive, the problem that space availability ratio is low, production efficiency is low, energy consumption is high.In the present invention, not only culture systems is relatively cheap, and the utilising efficiency of micro-algae to luminous energy, carbon source and nutrition is high, and the formation speed of secondary metabolite is fast, significantly improves unit occupied area Biomass yield and secondary metabolite productive rate.
Accompanying drawing explanation
Fig. 1 a-Fig. 1 e is the culture plate structural representation of this invention different IPs shell structure.
Fig. 2 is the structural representation of the cultivation unit of nucleocapsid structure of the present invention.
Fig. 3 is the schematic diagram of surface growth formula culture systems 1000a.
Fig. 4 is the schematic diagram of surface growth formula culture systems 1000b.
Embodiment
Below in conjunction with drawings and Examples, the invention will be further described; It should be noted that the implication that in the present invention, " rigidity " refers to refers to the material relative to canvas, cloth or sponge with larger hardness, include but not limited to the rigid of these building operation such as " permeable ceramic plate, cement plate, potter's clay fire plate, blue bricks plate, common brick plate and watt material plate ", the feature of these materials all can realize self-supporting under the state of vertical direction without special bracing frame and erosion resistance is strong.
The surface growth formula photosynthetic microorganism culture plate > of < nucleocapsid structure
The surface growth formula photosynthetic microorganism culture plate 1 of surface growth formula nucleocapsid structure of the present invention, as shown in Figure 1a, comprise two parts, namely be positioned at and be located at outside coverboard 11, this coverboard 11 is the many micropores platy structures be made up of rigid porous material, inside has the network-like microminiature tube pore structure of connection, the conduit 111 of several vertical direction is formed in this coverboard 11 inside, preferably, these conduits can be parallel to each other, run through the upper surface 112 of this coverboard 11, lower surface 113 (also can not run through); Several filled cores 12, for being filled in this each conduit 111, filled core 12 is made up of the material of absorb water, lock water and water permeability, such as, molecular sieve, non-woven fabrics, highly dense sponge, cotton, hydroscopic high-molecular resin and urethane water-retaining agent can be selected, and be not limited to these materials.Wherein, the magnitude setting of conduit 111 does not limit, but the evenness of wettability in order to the surface of coverboard 11, the better interval that each conduit 111 is distributed is less than 5cm.Wherein, the form of filled core 12 can be any form, and such as filled core 12 is the strip shown in Fig. 1 a or bulk, certainly can be only Powdered, any form conveniently inserted among conduit 111 such as particulate state, cotton-shaped or shred.
Described coverboard 11, between two conduits 111 formed a floor, its material can be identical with the material of coverboard 11, and one-body molded go out these floors and conduit 111, other material also can be adopted to make floor separately after, then install enter.The effect of said structure is, owing to separating between conduit 111, shell significantly reduces the probability that in a monoblock filled core, water column and hydrostaticpressure are formed, thus avoid seepage rate because of highly different and distinguish to some extent and coverboard 11 surface area water, make photosynthetic microorganism, as the phenomenon that algae kind cannot effectively be adhered to.
The culture plate 1 of above-mentioned core-shell type, its special coverboard adds filled core structure, not only can the double dominant of the filled core of " porous performance " and stronger " lock water and water retention property " that has of the external monolayer plate of comprehensive rigidity, solves prior art problem; Can also alleviate hydrostaticpressure, in reduce orifice, the formation in continuous water route, prevents surface area water, is more conducive to micro-algae apposition growth, promotes the product algae amount of culture plate unit surface simultaneously.
Wherein, coverboard 11 may be selected to be the existing building board of one or more in following material, as: cement plate, potter's clay fire plate, blue bricks plate, common brick plate and watt material plate; Simultaneously the substrate material of some microporosity and additive can be adopted to be made into the many microwell plates of a kind of hard, adopt additive to comprise binding agent, substrate material is molecular sieve, glass sand or green stone etc.; Additive is wherein aluminium stone, or the mixture of aluminium stone, sesbania powder and nitric acid.When adopting molecular sieve to make this coverboard 11, the better selection of molecular sieve has
the molecular sieve in aperture, such as molecular sieve beta molecular sieve or ZSM-5 molecular sieve.In one embodiment, the many microwell plates of described hard comprise molecular sieve and aluminium stone, and wherein said molecular sieve is 50-90 mass parts, and aluminium stone is 5-45 mass parts.In another embodiment, described coverboard comprises molecular sieve, aluminium stone, sesbania powder and nitric acid, and wherein said molecular sieve is 60-80 mass parts, and aluminium stone is 15-35 mass parts, and sesbania powder is 1-4 mass parts, and nitric acid is 1-4 mass parts.
Further, coverboard 11 surface, in roughness, to increase its surface-area, can allow more micro-algae attached to it, make to grow more micro-algae in unit volume.
Further, wherein the shape of the conduit 111 of this coverboard 11 is unrestricted.
Shown in Fig. 1 b, it is the distortion of Fig. 1 a, the horizontal section of Fig. 1 a conduit 111 is rectangle, the horizontal section of each conduit 111 of coverboard 11 is circular in Figure 1b, in addition, also trilateral or ellipse etc. can be set to, and filled core 12 can be respectively Powdered, cotton-shaped, shred or particulate state (not painting).
Shown in Fig. 1 a, each conduit 111 in its coverboard 11 is vertical to cross section along the surface, front and back two perpendicular to described coverboard, is uniform width, and filled core 12 is the strip of rule.In figure 1 c, can see, conduit 111 along perpendicular to coverboard 11 front and rear surfaces vertical to cross section for from top to bottom reducing for gradual, in Fig. 1 d, conduit 111 is vertically from top to bottom for staged reduces to cross section, distance between the front and back both side surface of conduit 111 inwall and this coverboard 11 is from top to bottom increased gradually, such structure design, it is the creepage distance by increasing below coverboard 11, to increase infiltration resistance, can the short transverse hydrostaticpressure infiltration that causes in counteracting portion be too fast to a certain extent, coverboard 11 surface-area water problem.
Shown in Fig. 1 e, described culture plate 1 can stacked base high be spliced into more large-area culture plate combination; So owing to short transverse there being polylith culture plate 1 overlapping, if then nutrient solution down seepage from the upper surface 112 of the culture plate 1 of the superiors, ooze the upper surface to culture plate 1 bottom gradually, culture plate 1 outside surface of the superiors will be caused to seep water very large, and orlop culture plate 1 outside surface can be just more dry, and the nutrient solution transmission speed on undermost culture plate 1 is comparatively slow, affects the speed of growth of algae on coverboard 11.
In order to address this problem, when four pieces of culture plates 1 as shown in fig. le stack together, the designated lane 114 being provided with and being specifically designed to nutrient solution flowing is run through in the coverboard 11 of each culture plate 1, for hollow tubular structure, those passages 114 are not filled core 12 when nutrient solution can be made to flow through each culture plate 1 and absorb, but directly by these passages 114, arrive adjacent lower one deck culture plate 1 upper surface 112; By the setting of these designated lanes 114, leaching rate and the seepage discharge of nutrient solution on each layer culture plate 1 in the combination of described culture plate can be balanced, make the growing state of each culture plate 1 outside surface algae in combination more meet expection, more balanced; Wherein, in passage 114, some water pipes (pipe) can also be set, be provided with highly dense sponge strip or the sliver 115 that absorbs water in the position that each culture plate 1 is connected with culture plate 1, make the water distribution of oozing out in passage 114 even.
In the present invention, the surface growth formula photosynthetic microorganism culture plate of nucleocapsid structure is by following methods manufacture (calling preparation method 1 in the following text): will have
the substance A (as molecular sieve microporous materials) in aperture and adhesive substance B (as aluminium stone class auxiliary) with ratio of quality and the number of copies about 70 parts: 25 parts mixing, adding addition of C (as sesbania and a small amount of additive of nitric acid) to said mixture carries out bonding, at 80-100 DEG C and normal pressure, bonding mixture is suppressed in a mold and forms the microporous platy structure of hard (technique of similar brickmaking), it is 3 ~ 40 μm that described many micropores coverboard has numerous aperture, be preferably the microporous pipeline structure (microporous pipeline formed between particle after molecular sieve pressing) of 3-10 μm, form several conduit 111 in the inside of this coverboard simultaneously, then, will have
the substance A in aperture (as molecular sieve porous material, simply can be processed into strip, also can not process) be closely filled in conduit 111 under normal temperature condition, form the surface growth formula photosynthetic microorganism culture plate 1 of nucleocapsid structure of the present invention.
In the present invention, the surface growth formula photosynthetic microorganism culture plate of nucleocapsid structure is by following methods manufacture (calling preparation method 2 in the following text): in the permeable ceramic plate with conduit 111, closely fills and has
the molecular sieve in aperture (can molecular sieve ZSM-5 or molecular sieve beta or the mixture of the two), forms the surface growth formula photosynthetic microorganism culture plate 1 of nucleocapsid structure of the present invention.
The cultivation unit > of the surface growth formula photosynthetic microorganism culture plate composition of < nucleocapsid structure
Fig. 2 shows cultivation unit 101 of the present invention, cultivates surface growth formula photosynthetic microorganism culture plate 1, stationary installation 2, nutrient solution retrieving arrangement 3, liquid feed device 4 that unit 101 comprises nucleocapsid structure.Described stationary installation 2 can comprise the assembly 21 and 22 that is relatively fixed, and forms the hole 23 can fixing culture plate 1 between fixation kit 21 and 22.Cultivate the array that unit 100 can comprise multiple culture plate 1, it forms larger plane culture plate assembly.Liquid feed device 4 is arranged on the top of culture plate 1, and liquid feed device 4 has the outlet such as hole, gap, thus the surface growth formula photosynthetic microorganism culture plate 1 making nutrient solution enter nucleocapsid structure with the form such as water clock, seepage is inner, is filled core 12 and absorbs and keep.The top lateral margin of culture plate 1 is salable in liquid feed device 4.As replacement, liquid feed device 4 can be vertically spaced apart with culture plate 1, drops to culture plate 1 to make nutrient solution from liquid feed device 4.The lower end side of culture plate 1 is salable in nutrient solution retrieving arrangement 3, contacts with the nutrient solution in nutrient solution retrieving arrangement 3, enables the nutrient solution in nutrient solution retrieving arrangement 3 infiltrate the bottom of this culture plate 1.
< culture systems embodiment 1>
Composition graphs 2 and Fig. 3 are described, Fig. 3 shows surface growth formula culture systems 1000a's of the present invention, surface growth formula culture systems 1000a can comprise multiple cultivation unit 101 as shown in Figure 2 and be arranged on and cultivates light supply apparatus 8 between unit 101, and provides the nutrient solution feedway 9 of nutrient solution for cultivating unit 101.
As shown in Figures 2 and 3, described stationary installation 2 comprises the assembly 21 and 22 that is relatively fixed, and forms the hole 23 can fixing culture plate 1 between fixation kit 21 and 22, and culture plate 1 can also upwards take out from hole 23.
Multiple cultivation unit 101 in Fig. 3 are set to be parallel to each other and are spaced a distance.Between every two cultivation unit 101, at least one light supply apparatus 8 is set.Light supply apparatus can be two-sided light source, irradiates the cultivation unit of both sides.Light supply apparatus 8 can be also single surface light source as required.
The surface growth formula culture systems 1000a of Fig. 2 comprises nutrient solution feedway 9, and this nutrient solution feedway 9 comprises cultivates liquid pool 6, recycle pump 7.
Nutrient solution retrieving arrangement 3 is for cultivating liquid pool 6 by oozing out from nucleocapsid structure culture plate 1 lower end or not collected by the nutrient solution that culture plate 1 absorbs and be back to by pipeline.When culture systems 1000a works, recycle pump 7 starts, by the nutrient solution cultivated in liquid pool 6 by Cemented filling to liquid feed device 4, nutrient solution is supplied nucleocapsid structure culture plate 1 by liquid feed device 4, nucleocapsid structure culture plate 1 is absorbed nutrient solution and is transported to the surface of nucleocapsid structure culture plate 1 by the tiny aperture of its inside, be provided as the nutrition needed for micro algae growth, and nucleocapsid structure culture plate 1 end contacts with the nutrient solution in nutrient solution retrieving arrangement 3 simultaneously, nutrient solution infiltrates the subregion of nucleocapsid structure culture plate 1 lower end by wicking action, nucleocapsid structure culture plate 1 is made to be in leather hard.
Nutrient solution feedway 9 also can comprise carbonic acid gas and be mixed into device (not shown), for being mixed in nutrient solution by carbonic acid gas.
< culture systems embodiment 2>
Composition graphs 2 and Fig. 4 illustrate another culture systems 1000b of the present invention.Fig. 4 shows surface growth formula culture systems 1000b of the present invention.Similar with the embodiment shown in Fig. 3, culture systems 1000b also can comprise the cultivation unit 101 of one or more Fig. 2.Particularly, culture systems 1000b can comprise nucleocapsid structure culture plate 1, stationary installation 2, nutrient solution retrieving arrangement 3, liquid feed device 4, cultivate liquid pool 6, pressure-pot 11, air compressing source of the gas 12, liquidometer 13, stopping valve 14, stopping valve 15, reducing valve 16, vent valve 17 and tensimeter 18.
The surface growth formula culture systems 1000b of Fig. 4 comprises nutrient solution circulation device 9b, optional employing air compressing mode in described nutrient solution circulation device 9b.This air compressing mode equipment therefor is this area other pressure pump replacement device optional, optional but be not limited to comprise pressure-pot 11, air compressing source of the gas 12, liquidometer 13 and mating valve, preferred stopping valve 14,15, reducing valve 16 etc.By by pressure-pot 11 lay in enough nutrient solutions condition under, valve 15 is cut in its upstream in circulation device 9b close, stopping valve 14 is opened, nutrient solution in pressure-pot 11 is made to be delivered to liquid feed device 4 in circulation device 9b by air compressing source of the gas 12, tensimeter 18 can be selected to monitor, liquid feed device 4 is with spray, the mode of water clock or seepage provides nutrient solution to nucleocapsid structure culture plate 1, thus make the nutrient solution containing cultivating desired nutritional material infiltrate whole nucleocapsid structure culture plate 1, nutrient solution retrieving arrangement 3 is positioned at the below of nucleocapsid structure culture plate 1, do not flow to nutrient solution retrieving arrangement 3 by the nutrient solution that nucleocapsid structure culture plate 1 absorbs, nutrient solution in nutrient solution retrieving arrangement 3 is flow to by back of pipeline and cultivates liquid pool 6.
the operation of hydrobiont surface growth formula culture systems
The surface growth formula culture systems 1000a of the embodiment of the present invention 1 can operate as follows:
-by nutrient solution feedway 9, liquid feed device 4 is had to comprise CO
2the nutrient solution of microvesicle, liquid feed device 4 provides nutrient solution in the mode of spray, water clock or seepage to nucleocapsid structure culture plate 1;
-nucleocapsid structure culture plate 1 is kept in touch with the nutrient solution in nutrient solution retrieving arrangement 3, thus make containing CO
2the nutrient solution of microvesicle infiltrates nucleocapsid structure culture plate 1;
-in the micro-algae of the surface seeding of nucleocapsid structure culture plate 1;
-utilize described light supply apparatus 8 to irradiate described nucleocapsid structure culture plate 1, and the ambient moisture arranged around culture systems 1000a and temperature, temperature is generally 20-35 DEG C, and humidity, in the scope of 50-80%, makes micro algae growth.
The surface growth formula culture systems 1000b of the embodiment of the present invention 2 can operate as follows:
1. vent valve 17 is opened, and stopping valve 14 and stopping valve 15 are closed, and reducing valve 16 pressure is adjusted to 0MPa, and reducing valve 16 requires within the scope of 0-1.0MPa adjustable;
2. cultivating the nutrient solution adding certain volume amount in liquid pool 6;
3. open stopping valve 15, nutrient solution is from cultivation liquid pool 6 feed pressure tank 11, and liquid level is below the line entry that stopping valve 14 is connected;
4. close stopping valve 15 and vent valve 17, open stopping valve 14, the pressure of adjustment reducing valve 16, the nutrient solution in pressure-pot 11 can flow into liquid feed device 4 gradually, for nucleocapsid structure culture plate 1 provide micro-algae grow required nutrient solution.Wherein, the pressure that the micro algae growth situation carried according to nucleocapsid structure culture plate 1 and tensimeter 18 show, by the pressure adjusting of reducing valve 16 to suitable value;
5. the nutrient solution flowing through nutrient solution retrieving arrangement 3 turns back to be cultivated in liquid pool 6, carries out Real-Time Monitoring simultaneously, if composition does not reach fostering requirement, supplement corresponding nutrient solution composition in time to the composition cultivated in liquid pool 6;
6. be provided with the liquidometer 13 of low Level Detection in pressure-pot 11, after liquid level is lower than the liquid level set, opened by vent valve 17, stopping valve 15 is opened, and stopping valve 14 is closed, and nutrient solution is from cultivation liquid pool 6 feed pressure tank 11.The process of repeating step 3 to step 5, realizes algae non-interruptible supply nutrient solution micro-on nucleocapsid structure culture plate 1;
7., after nutrient solution infiltrates whole nucleocapsid structure culture plate 1, the surface of nucleocapsid structure culture plate 1 is inoculated micro-algae;
8. utilize light supply apparatus 8 to irradiate nucleocapsid structure culture plate 1, and the ambient moisture arranged around culture systems 100 and temperature, temperature is generally 20-35 DEG C, and humidity, in the scope of 50-80%, makes hydrobiont grow.
The system of the embodiment of the present invention 2, by tensimeter 18, reducing valve 16 and liquidometer 13, comparatively accurately can control feed rate and the speed of nutrient solution according to micro algae growth situation, realize the object precisely controlled.
Be below that cultivation unit of the present invention and prior art are compared, embodied to make this technique effect of the present invention further:
The preparation of embodiment 1 nucleocapsid structure culture plate of the present invention and contrast
The preparation example of nucleocapsid structure culture plate 1 of the present invention:
Nucleocapsid structure culture plate of the present invention is obtained 1. according to aforementioned preparation process 1;
According to preparation method 2, prepare nucleocapsid structure culture plate of the present invention 2. with molecular sieve ZSM-5.
Described nucleocapsid structure culture plate size 1. and is 2. 1 meter * 2 meters * 3 centimetres.
3 groups of contrasts are set:
1st group: contrast canvas (32), size and described nucleocapsid structure culture plate 1 quite, sandwich of layers is promising sponge sucks in water material.
2nd group: according to aforesaid preparation method 1, make a porous plate with molecular sieve and aluminium stone etc., but do not form conduit 111, obtain control board 1.;
3rd group: 2. the blocks that the molecular sieve ZSM-5 of clamping one monoblock forms in the middle of two pieces of permeable ceramic plates, obtain control board.
1. and 2. suitable the size of above-mentioned 3 groups of control boards and the surface growth formula photosynthetic microorganism culture plate of described nucleocapsid structure.
Embodiment 2 water-intake rate compares
Table 1. water-intake rate compares
| Object | Dry weight (g) | Weight (g) after water suction | Water-intake rate (%) |
| Nucleocapsid structure culture plate 1. | 95 | 227 | 139 |
| Nucleocapsid structure culture plate 2. | 102 | 239 | 135 |
| Contrast canvas | 130 | 236 | 81.5 |
| Control board 1. | 110 | 245 | 123 |
| Control board 2. | 100 | 236 | 136 |
From table 1,1. and 2. water-intake rate is respectively apparently higher than contrast canvas and control board 1. for nucleocapsid structure culture plate of the present invention, with control board water-intake rate 2. quite or a little more than control board 2., may be due to control board structure 2. in the middle of the moisture of the molecular sieve water conservation layer reason of easily oozing out from bottom.
The infiltration of embodiment 3 culture surface and algae kind adhesion property compare
With identical water supply rate to aforesaid nucleocapsid structure culture plate 1., 2., contrast canvas, control board 1., control board upper end side 2. drips, and continues after 5 minutes, observe or to feel under each culture plate surface by feel less than 1/5 position infiltration ponding situation;
Algae kind is sprayed or is coated on the surface of dry each culture plate, with identical water supply rate to aforesaid nucleocapsid structure culture plate 1., 2., contrast canvas, control board 1., control board upper end side 2. drips, continue after 5 minutes, observe the adhesion condition of the position algae kind of under each culture plate surface less than 1/5 again, be compared to following table (table 2):
The aqueous comparison of table 2.
Embodiment 4 culture experiment
4.1. nutrient solution composition
Consist of example with the nutrient solution that chlorella is cultivated, see the following form 2.
The nutrient solution composition that table 3 chlorella is cultivated
| Component | Usage quantity | Mother liquor |
| NaNO 3 | 1m L/L | 25g/100ml dH 2O |
| K 2HPO 4 | 1m L/L | 7.5g/100ml dH 2O |
| MgSO 4·7H 2O | 1m L/L | 7.5g/100ml dH 2O |
| CaCl 2·2H 2O | 1m L/L | 2.5g/100ml dH 2O |
| KH 2PO 4 | 1m L/L | 17.5g/100ml dH 2O |
| NaCl | 1m L/L | 2.5g/100ml dH 2O |
| Na 2CO 3 | 1m L/L | 0.2g/100ml dH 2O |
| FeCl 3·6H 2O | 1m L/L | 0.05g/100ml dH 2O |
| EDTA-Fe | 1m L/L | |
| Trace metal solutions | 1m L/L | |
| Soil extract | 40m L/L |
The formula of table 4 trace metal solutions
| Component | Mother liquor |
| H 3BO 3 | 2.86g/100m L dH 2O |
| MnCl 2·4H 2O | 1.86g/100m L dH 2O |
| ZnSO 4·7H 2O | 0.22g/100m L dH 2O |
| Na 2MO 4·2H 2O | 0.39g/100m L dH 2O |
| CuSO 4·5H 2O | 0.08g/100m L dH 2O |
| Co(NO 3) 2·6H 2O | 0.05g/100m L dH 2O |
In addition, in nutrient solution, the preparation method of EDTA-Fe is: get 4.1mL concentrated hydrochloric acid distilled water diluting to 50mL, make 1N HCl; Take 0.93g EDTA-Na
2and be dissolved in 500mL distilled water, make 0.1N EDTA-Na
2solution; Take FeCl
36H
2o 0.90g is also dissolved in the above-mentioned 1N HCl of 10mL, then with 10ml 0.1N EDTA-Na
2solution mixes, and adds distilled water diluting to 1000m L.Soil extract liquid making method is: get the garden soil 200g not executing overfertilization and be placed in beaker or triangular flask, add distilled water 1000m L, bottleneck porous plug seals, boiling water heats 3 hours in a water bath, and cooling, precipitates 24 hours, this process carries out 3 times continuously, then filter, get supernatant liquor, save backup in 4 DEG C of refrigerators after sterilizing in high-pressure sterilizing pot.
According to the formula of as above table 3 and table 4, chlorella nutrient solution used is cultivated in preparation.Be stored in container stand-by.
4.2. the assembling of nucleocapsid structure culture systems of the present invention and control group
According to the culture systems shown in Fig. 3-4, connect respectively and cultivate unit 101 and nutrient solution feedway 9, pipeline is connected to liquid feed device 4, nutrient solution retrieving arrangement 3 is connected to cultivates liquid pool 6, open pump 7, the nutrient solution cultivated in liquid pool 6 is delivered to each nucleocapsid structure culture plate 1., in the liquid feed device 4 of nucleocapsid structure culture plate 2. set by upper end, and liquid feed device 4 drips to the top lateral margin of culture plate 1 in the mode of drippage.
Again according to the culture systems shown in Fig. 3, just 1. nucleocapsid structure culture plate is wherein changed into the nucleocapsid structure culture plate of same size 2., contrast canvas, control board 1. and control board 2..
the operation of 4.3 culture systems
With reference to described in Fig. 3, surface growth formula of the present invention is cultivated unit 1000a and is used for cultivating micro-algae, can comprise the following steps:
The culture systems of the culture systems shown in-service chart 3 and control group;
-at nucleocapsid structure culture plate 1., 2., contrast canvas, control board 1. and the micro-algae of control board surface seeding 2.;
Utilize described light supply apparatus 8 irradiate described nucleocapsid structure culture plate 1., 2., contrast canvas, control board 1., control board 2., and the envrionment temperature arranged around culture systems 1000a is generally 20-35 DEG C, and humidity, in the scope of 50-80%, makes micro algae growth.
5. culture Comparative result
After above-mentioned culture systems cultivates 24,48 and 72 hours, respectively from the surface growth formula photosynthetic microorganism culture plate of the nucleocapsid structure of the present invention of same homalographic 1. and 2., contrast canvas, control board 1., 2. control board harvesting microalgae culture, to weigh algae mud, after 70 DEG C of drying and dehydratings, take dry weight and carry out dry biomass weight ratio comparatively.
Implement the results contrast of the surface growth formula culture systems of the embodiment of the present invention 1:
Table 4 cultivation results compares
From table 4, micro-algae dry biomass is all significantly higher than contrast canvas in the output of nucleocapsid structure culture plate 1 of the present invention, apparently higher than obtain according to preparation method 1 without conduit many microwell plates (control board 1.) and with permeable pottery and molecular sieve composition three-decker control board 2..
Claims (27)
1. a surface growth formula photosynthetic microorganism culture plate for nucleocapsid structure, its feature comprises:
Be located at outside coverboard, this coverboard is the many micropores platy structure be made up of rigid porous material;
Filled core, be filled in this coverboard inner, described filled core is water conservation water-permeable material.
2. the surface growth formula photosynthetic microorganism culture plate of nucleocapsid structure as claimed in claim 1, is characterized in that, forms at least one conduit in described coverboard, fills described filled core in described conduit.
3. the surface growth formula photosynthetic microorganism culture plate of nucleocapsid structure as claimed in claim 2, it is characterized in that, described conduit comprises multiple, parallel and longitudinally run through upper and lower two end faces of described coverboard, between every two conduits, there is a floor, by forming region between two conduits every.
4. the surface growth formula photosynthetic microorganism culture plate of nucleocapsid structure as claimed in claim 2, it is characterized in that, described coverboard select in following material one or more make: permeable ceramic plate, cement plate, potter's clay fire plate, blue bricks plate, common brick plate and watt material plate.
5. the surface growth formula photosynthetic microorganism culture plate of nucleocapsid structure as claimed in claim 4, it is characterized in that, described filled core select in following material one or more make: molecular sieve, glass sand, non-woven fabrics, highly dense sponge, cotton, hydroscopic high-molecular resin and urethane water-retaining agent.
6. the surface growth formula photosynthetic microorganism culture plate of nucleocapsid structure according to claim 5, is characterized in that, the form of described filled core is strip, bulk, Powdered, particulate state, cotton-shaped or shred.
7. the surface growth formula photosynthetic microorganism culture plate of nucleocapsid structure as claimed in claim 3, it is characterized in that, the horizontal section of described conduit is circle, trilateral, track type, rectangle or ellipse.
8. the surface growth formula photosynthetic microorganism culture plate of nucleocapsid structure as claimed in claim 3, it is characterized in that, described conduit is along the vertical section perpendicular to the surface, front and back two of described coverboard, it is from top to bottom run-in index, or gradually from top to bottom to reduce or step type reduces, conduit inwall is from top to bottom increased gradually apart from the both side surface of this coverboard.
9. the surface growth formula photosynthetic microorganism culture plate of nucleocapsid structure as claimed in claim 5, it is characterized in that, described molecular sieve has
the aperture of ~ 2nm.
10. the surface growth formula photosynthetic microorganism culture plate of nucleocapsid structure as claimed in claim 9, it is characterized in that, described molecular sieve is beta molecular sieve or ZSM-5 molecular sieve.
The surface growth formula photosynthetic microorganism culture plate of 11. nucleocapsid structures as claimed in claim 2, is characterized in that, described each coverboard can build folded splicing and combining, and described each coverboard inside is provided with the designated lane directly passed through for liquid.
12. 1 kinds of surface growth formulas cultivate unit, and its feature comprises:
The surface growth formula photosynthetic microorganism culture plate of at least one nucleocapsid structure as described in claim 1-11, its surface is for the growth of attachment photosynthetic microorganism;
At least one liquid feed device, described liquid feed device is for providing the nutrient solution needed for photosynthetic microorganism growth to the surface growth formula photosynthetic microorganism culture plate of described nucleocapsid structure, described nutrient solution is absorbed by the surface growth formula photosynthetic microorganism culture plate of described nucleocapsid structure and infiltrates into the surface of the surface growth formula photosynthetic microorganism culture plate of this nucleocapsid structure;
At least one nutrient solution retrieving arrangement, the surface growth formula photosynthetic microorganism culture plate lower end of described nucleocapsid structure is located at by described nutrient solution retrieving arrangement, to be oozed out the surface growth formula photosynthetic microorganism culture plate lower end of each nucleocapsid structure or not collected by the nutrient solution that the surface growth formula photosynthetic microorganism culture plate of nucleocapsid structure absorbs.
13. surface growth formulas according to claim 12 cultivate unit, and it is characterized in that, described liquid feed device system covers at the top of the surface growth formula photosynthetic microorganism culture plate of described nucleocapsid structure.
14. surface growth formulas according to claim 12 cultivate unit, it is characterized in that, described liquid feed device is located at the top position, top of the surface growth formula photosynthetic microorganism culture plate of this nucleocapsid structure with interval mode, described liquid feed device provides nutrient solution to the surface growth formula photosynthetic microorganism culture plate of this nucleocapsid structure in the mode of spray, water clock or seepage.
15. surface growth formulas according to claim 12 or 13 or 14 are cultivated unit and be it is characterized in that, described cultivation unit also comprises at least one stationary installation, and this stationary installation is used for the surface growth formula photosynthetic microorganism culture plate of described nucleocapsid structure to be fixed on a predetermined position in upright mode.
16. 1 kinds of surface growth formula culture systems, this culture systems comprises:
An array be made up of the cultivation unit described in any one of claim 12-15, and
For providing the nutrient solution feedway of nutrient solution to the liquid feed device of the cultivation unit in described array;
Wherein, described nutrient solution feedway comprises cultivates liquid pool and cycle power device, and described cultivation liquid pool is for storing nutrient solution, and described cycle power device is used for the nutrient solution in described cultivation liquid pool to be delivered to respectively this liquid feed device.
17. surface growth formula culture systems according to claim 16, is characterized in that, described cycle power device is a pump, and this pump is located at this cultivation liquid pool and is connected on the pipeline of respectively this liquid feed device.
18. surface growth formula culture systems according to claim 16 or 17, is characterized in that, what described nutrient solution feedway also comprised a carbonic acid gas is mixed into device, described in be mixed into device just carbonic acid gas be mixed into in described nutrient solution cultivation pool.
19. surface growth formula culture systems according to claim 16, it is characterized in that, described cycle power device comprises a pressure-pot, an air compressing source of the gas, wherein this pressure-pot is connected with this cultivation pool, nutrient solution in this cultivation pool inputs in this pressure-pot temporary, this air compressing source of the gas is connected to this pressure-pot, for carrying out pressure adjustment to pressure-pot inside, this pressure-pot be connected to respectively this liquid feed device by pipeline, open this air compressing source of the gas to this pressure-pot supercharging, make the nutrient solution in pressure-pot be delivered to respectively this liquid feed device.
20. surface growth formula culture systems according to claim 19, it is characterized in that, this pressure-pot connects a liquidometer, and the pipeline that this air compressing source of the gas is connected to this pressure-pot is provided with stopping valve, reducing valve, and the pipeline that this pressure-pot is connected to respectively this liquid feed device is provided with a tensimeter.
21. surface growth formula culture systems according to claim 16 or 17 or 19 is characterized in that, the surface growth formula photosynthetic microorganism culture plate lower end of this nucleocapsid structure is oozed out or do not collected by the nutrient solution that the surface growth formula photosynthetic microorganism culture plate of this nucleocapsid structure absorbs and be back to cultivation liquid pool by pipeline by described nutrient solution retrieving arrangement.
22. surface growth formula culture systems according to claim 21, is characterized in that, the surface growth formula photosynthetic microorganism culture plate of described nucleocapsid structure contacts with the nutrient solution in nutrient solution retrieving arrangement.
23. surface growth formula culture systems according to claim 16 or 17 or 19 is characterized in that, described cultivation unit is at least two, is provided with light supply apparatus between adjacent cultivation unit, and this light supply apparatus and described cultivation unit are arranged abreast; Or the parallel placement of described each cultivation unit forms array, and a light supply apparatus is located at the side of this array, with respectively this cultivation unit is vertical.
24. surface growth formula culture systems according to claim 16 or 17 or 19 is characterized in that, described light source is natural light or source of artificial light, and described source of artificial light is two-sided light source or single surface light source.
25. 1 kinds of methods using the surface growth formula culture systems described in any one of claim 16-24 to cultivate photosynthetic microorganism, comprise the following steps:
A) nutrient solution cultivated in liquid pool is delivered to respectively this liquid feed device by described cycle power device, described liquid feed device provides nutrient solution in the mode of spray, water clock or seepage to the surface growth formula photosynthetic microorganism culture plate of nucleocapsid structure, thus makes the surface growth formula photosynthetic microorganism culture plate infiltrating whole nucleocapsid structure containing the nutrient solution cultivating desired nutritional material;
B) on the surface growth formula photosynthetic microorganism culture plate of described nucleocapsid structure, photosynthetic microorganism is inoculated;
C) ambient moisture and temperature are set, under the photon intensity that applicable photosynthetic microorganism grows, make photosynthetic microorganism carry out photosynthesis, growth and breeding.
The method of 26. cultivation photosynthetic microorganisms according to claim 25, it is characterized in that, step a) in, also comprise and the surface growth formula photosynthetic microorganism culture plate by described nucleocapsid structure oozes out or is not recycled to the step of cultivating in liquid pool by the nutrient solution that the surface growth formula photosynthetic microorganism culture plate of nucleocapsid structure absorbs, the nutrient solution composition in liquid pool is cultivated in monitoring simultaneously, supplements the nutrients in time to cultivation liquid pool.
The method of 27. cultivation photosynthetic microorganisms according to claim 25, is characterized in that, described photosynthetic microorganism comprises chlorella, spirulina, green alga, grid algae, Isochrysis galbana, micro-plan ball algae or blood cell algae.
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| CN201410599353.8A CN104328029A (en) | 2014-10-30 | 2014-10-30 | Surface growth type photosynthetic microorganism culture plate having core-shell structure, culture unit, system and culture method |
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| CN201410599353.8A CN104328029A (en) | 2014-10-30 | 2014-10-30 | Surface growth type photosynthetic microorganism culture plate having core-shell structure, culture unit, system and culture method |
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| CN105797572A (en) * | 2016-03-23 | 2016-07-27 | 凯天环保科技股份有限公司 | Reaction bed for purifying CO2 through biological method |
| CN105854581A (en) * | 2016-03-23 | 2016-08-17 | 凯天环保科技股份有限公司 | CO2 purifier |
| CN111777192A (en) * | 2019-04-03 | 2020-10-16 | 广源造纸股份有限公司 | Trickling filter and aeration tank combined structure of wastewater treatment system |
| CN111777191A (en) * | 2019-04-03 | 2020-10-16 | 广源造纸股份有限公司 | Microbial cultivation module of wastewater treatment system |
| WO2022077713A1 (en) * | 2020-10-17 | 2022-04-21 | 徐州创之社通用技术产业研究院有限公司 | Microbial water purification brick |
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| CN105797572A (en) * | 2016-03-23 | 2016-07-27 | 凯天环保科技股份有限公司 | Reaction bed for purifying CO2 through biological method |
| CN105854581A (en) * | 2016-03-23 | 2016-08-17 | 凯天环保科技股份有限公司 | CO2 purifier |
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| CN111777192A (en) * | 2019-04-03 | 2020-10-16 | 广源造纸股份有限公司 | Trickling filter and aeration tank combined structure of wastewater treatment system |
| CN111777191A (en) * | 2019-04-03 | 2020-10-16 | 广源造纸股份有限公司 | Microbial cultivation module of wastewater treatment system |
| WO2022077713A1 (en) * | 2020-10-17 | 2022-04-21 | 徐州创之社通用技术产业研究院有限公司 | Microbial water purification brick |
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Application publication date: 20150204 |