CN104328030A - Surface growth type culture plate having sandwich structure, surface growing culture system and surface growing culture method - Google Patents
Surface growth type culture plate having sandwich structure, surface growing culture system and surface growing culture method Download PDFInfo
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Abstract
The invention relates to a surface growth type culture plate having a sandwich structure which comprises outer plates and a core part which is located inside the outer plates, wherein the outer plate is made from rigid water adsorption seepy material plate and the core part is made from a water absorption water-locking material such as a molecular sieve, glass sands, a non-woven fabric, high density sponge, cotton cloth, high molecular water-absorbent resin, super absorbent fibers or a polyurethane water absorbent. The core part is arranged between the outer plates on two sides. The invention further relates to a culture unit which comprises the culture plate, a surface growing culture system and a method for culturing aquatic organisms by using the surface growing culture system. The invention aims at solving the problem that a photobioreactor system for microalgae culture is high in manufacturing and maintenance costs, low in space utilization ratio and low in production efficiency. According to the invention, not only is the culture system economic and cheap, but also the utilization efficiency of light energy, carbon sources and nutrients by microalgae is high and the synthesizing and accumulating speed of secondary metabolites is fast, so that the biomass yield and the secondary metabolism yield in unit floor area or the unit reactor volume or area are improved to a great extent.
Description
Technical field
The surface growth formula culture plate that the present invention relates to sandwich structure is with the cultivation unit, the surface growth formula culture systems that comprise this culture plate and utilize surface growth formula culture systems to cultivate hydrobiological method.
Background technology
Micro-algae refers to and can carry out photosynthetic aquatic planktonic algae.Certain is the rich in proteins of algae own slightly, can as aquatic feed or animal and fowl fodder (as spirulina); Prior, certain slightly algae can synthesize secondary metabolite in a large number under given conditions, as grease, carotenoid, polysaccharide etc., these materials have the biologically active substance of high economic worth often, can be used in the fields such as functional food, foodstuff additive, pharmacy, bioenergy.Particularly extract microalgae grease by micro-algae large scale culturing, and then conversion production biofuel is considered to one of most important approach solving bioenergy production and carbon fixation and emission reduction.
Microdisk electrode has history decades, and current industrialization microdisk electrode is liquid submersion, using the Supporting Media that large water gaging is lived as micro-algae.Mainly comprise open cultivation pool and Closed photobioreactor (photo-bioreactor, PBR) two kinds of forms.The advantage of open cultivation pool is that the cost built and run is lower.But because the illuminating area/volume in open pond is smaller, fluid surface mixes poor with bottom, only have top layer frustule can accept more sufficient illumination, and pond floor cells is often difficult to receive abundant illumination; Secondly, it is more shallow that the operation depth of water is cultivated in open pond, generally only has 10-30 centimetre, and gas-liquid contact time when mending carbon that makes to ventilate is short, mends carbon efficiencies low, dissolved carbon dioxide (CO in nutrient solution
2) deficiency photosynthesis is restricted, another is that open intensification is slow, can not be raised to the best temperature of enzymic activity 25 DEG C at short notice, often misses the best moment that light utilizes; And temperature declines too slow naturally in Night Service formula pond, make the respiration that frustule still keeps vigorous, the energy expenditure that daytime stores is fallen, therefore makes metabolite content useful in frustule too low.Therefore the open pond cultured cells speed of growth is all lower with culturing cell density, and it also takes up an area greatly in addition.In contrast, PBR-as be adopt light transmissive material (as glass, synthetic glass, plastics film etc.) the thin thin structure made, because optical path is little, culture system illuminating area/volume ratio is comparatively large, so cell illumination is more abundant.Meanwhile, mend carbon gas and liquid contact time long, nutrient solution concentration of ordinary dissolution is higher, and thus all comparatively open culture pond is high for vitro growth rates and culture density.But such PBR involves great expense usually, running cost is high, difficult in maintenance, be difficult to maximize, productive rate is at 5-30g/m
2/ d, well below theoretical expected value 100-200g/m
2/ d, does not reach the Theoretical Calculation target of industrialization, and the efficiency of light energy utilization this micro-algae large scale culturing low realizes the most important direct restraining factors of industrialization and still has much room for improvement.
In recent years, there is a kind of half-dried culture systems, existing patent report, all adopt one or more in filter paper, filter cloth, plastic foam, fabric (such as canvas) as substrate material, but such soft material possesses following shortcoming: 1) physical strength is low, need complicated support, amplification on improper large size and spatial altitude, short thin thin structure can only be made, and often can self-supporting, its lock water-based of the more much higher Porous materials of rigidity is more limited again, cannot meet the nutrition liquid measure needed for micro algae growth well; 2) surface moisture such as existing filter cloth and fabric is uneven, and micro algae growth is insecure; And 3) existing filter cloth and fabric etc. are not resistance to recycles, and needs often to change, increase cost, 4) impurity such as fiber that easily drop such as existing filter cloth and fabric; In addition, 5) these flexible materialss are when as culture plate surfacing, and its aspect such as bio-compatibility and chemical resistance all has certain limitation.Therefore this type of culture systems does not enter the possibility of scale operation at present.
In view of the above-mentioned shortcoming of prior art, the present invention intends adopting a kind of novel surface growth formula culture systems and surface growth formula cultural method.
Summary of the invention
For solving the problem, one aspect of the present invention provides a kind of surface growth formula culture plate of sandwich structure, it core comprising outside plate and be positioned at outside plate, wherein, this outside plate is rigidity pervious material flitch, and wherein this core is water suction lock water material, such as molecular sieve, glass powder or glass sand, this core is located between this both sides outside plate.
Be in embodiment at one of the present invention, described outside plate is provided with the web member embedding this core, and this core is fixed between this both sides outside plate.
Preferably, wherein molecular sieve, glass powder or glass sand core are that molecular sieve, glass powder or glass sand are forced together platy structure in advance by certain pressure in advance, then are arranged between this both sides outside plate, and core strength is less, is mainly used in water-absorbing-retaining effect.The intensity of this outside plate is comparatively large, is mainly used in directly in the face of outside atmosphere, and for micro-algae attachment, can absorb ooze out into outside surface for the nutrient solution absorbed by core.
Be in embodiment at one of the present invention, described outside plate is permeable ceramic plate, cement plate, potter's clay fire plate, green stone, blue bricks plate, common brick plate or watt material plate.
Preferably, described outside plate is adopt the matrix of microporosity and tackiness agent pressing to form hard porous sheet material, and described substrate material is molecular sieve, tackiness agent comprises aluminium stone.
Preferably, described core is molecular sieve, glass sand, non-woven fabrics, highly dense sponge, cotton, hydroscopic high-molecular resin, super water-absorbent fiber or urethane water-retaining agent.
Being in embodiment at one of the present invention, imbedding skeleton in described core to support this culture plate and for guiding nutrient solution.
Preferably, described skeleton is the form of small sized pipeline, network etc.
Preferably, the surface of described outside plate is set to male and fomale(M&F), to increase its surface-area, can adhere to more algae grain; Wherein male and fomale(M&F) is corrugated surface.
The present invention additionally provides a kind of cultivation unit simultaneously, and this cultivation unit comprises surface growth formula culture plate and the stationary installation thereof of the sandwich structure of aforementioned arbitrary embodiment.
Preferably, this cultivation unit comprises the surface growth formula culture plate of multiple described sandwich structure, and the surface growth formula culture plate of the plurality of sandwich structure forms single writing board shape.
On the basis of above-mentioned design, the invention still further relates to a kind of surface growth formula culture systems, it comprises: the cultivation unit of at least one aforementioned arbitrary embodiment and nutrient solution circulation device.
Preferably, described cultivation unit is at least two, is provided with light supply apparatus between adjacent cultivation unit, and this light supply apparatus and described cultivation unit are arranged abreast; Or the parallel placement of described each cultivation unit forms array, and a light supply apparatus is located at the side of this array, with respectively this cultivation unit is vertical.
Preferably, described light source is natural light or source of artificial light, and described source of artificial light is two-sided light source or single surface light source.
Preferably, the wavelength of light source is in 660-680nm scope.
Preferably, described nutrient solution circulation device comprises cultivation liquid pool, recycle pump, liquid feed device and nutrient solution retrieving arrangement, nutrient solution in described cultivation liquid pool is delivered to described liquid feed device by recycle pump, described liquid feed device is located at above the surface growth formula culture plate of described sandwich structure, and with spray, the mode of water clock or seepage provides nutrient solution to the surface growth formula culture plate of sandwich structure, the below of the surface growth formula culture plate of described sandwich structure is located at by described nutrient solution retrieving arrangement, oozed out or unabsorbed nutrient solution by the surface growth formula culture plate of sandwich structure in order to collect, and be back to cultivation liquid pool by pipeline.
Preferably, what described nutrient solution circulation device also comprised nutritive substance needed for cultivation is mixed into device.
Preferably, the surface growth formula culture plate of described sandwich structure contacts with the nutrient solution in nutrient solution retrieving arrangement.
Preferably, described nutrient solution circulation device comprises cultivation liquid pool, pressure-pot, air compressing source of the gas, liquid feed device and nutrient solution retrieving arrangement, described pressure-pot fluid inlet is connected with described cultivation liquid pool, for temporary a certain amount of nutrient solution, this air compressing source of the gas is connected with this pressure-pot inlet mouth can to pressure-pot supercharging, nutrient solution is exported from liquid outlet, the liquid outlet of this pressure-pot is connected with this liquid feed device, described liquid feed device is located at above the surface growth formula culture plate of described sandwich structure, and with spray, the mode of water clock or seepage provides nutrient solution to the surface growth formula culture plate of sandwich structure, the below of the surface growth formula culture plate of described sandwich structure is located at by described nutrient solution retrieving arrangement, oozed out or unabsorbed nutrient solution by the surface growth formula culture plate of sandwich structure in order to collect, and be back to cultivation liquid pool by pipeline.
Preferably, described pressure-pot connects a liquidometer, and the pipeline that this air compressing source of the gas is connected to this pressure-pot is provided with reducing valve, stopping valve; The pipeline that this pressure-pot is connected to liquid feed device is provided with tensimeter; The venting port of this pressure-pot is provided with stopping valve; Pipeline between this cultivation liquid pool and this pressure-pot is provided with stopping valve.
Based on above-mentioned culture systems, the invention still further relates to one and utilize surface growth formula culture systems to cultivate hydrobiological method, the method comprises the steps:
A) by nutrient solution circulation device, the nutrient solution cultivated in liquid pool is delivered to the surface growth formula culture plate of the sandwich structure of each cultivation unit, thus makes nutrient solution infiltrate the surface growth formula culture plate of whole sandwich structure;
B) the surface growth formula culture plate surface seeding hydrobiont of described sandwich structure;
C) ambient moisture and temperature are set, under the photon intensity that applicable hydrobiont grows, hydrobiont are grown.
Wherein, step a) in, the nutrient solution composition in liquid pool is cultivated in monitoring, supplements nutrient solution in time.
Preferably, described hydrobiont is micro-algae.
Preferably, described micro-algae comprises chlorella, spirulina, green alga, grid algae, Isochrysis galbana, micro-plan ball algae or blood cell algae.
Preferably, ambient moisture is 65%-75%, and temperature is 26-28 DEG C.
For carrying out the cultivation of surface growth formula, the present invention by adopting a kind of core having rigidity water-permeable outside plate and have water conservation water permeability, such as, adopts molecular sieve, glass powder or glass sand to make the surface growth formula culture plate of the sandwich structure of core.This rigidity water-permeable outside plate is used for oozing out nutrient solution, and this core is for keeping nutrient solution.
The surface growth formula culture plate of this sandwich structure has good water suction/permeability performance, therefore its nutrient solution absorbed can be got up by molecular sieve core deposit and provide supply for rigid porous outside plate, the aperture that its core and outside plate have allows water molecules fully to circulate at the internal network of material, and due to the induced effect of water flow, the surface that nutrition small-molecule substance needed for cultivation all arrives outside plate by diffusion realizes being uniformly distributed, and micro-algae algae kind that particle diameter is larger, be not easy the inside oppositely infiltrated to culture plate, again due to the wetting ability of above-mentioned culture plate, its electric charge allows a large amount of micro-algae algae kind attachments (on the cliff of the growth such as similar liver moss in water).In addition, the density ratio of this growth plate is lower, intensity and rigidity higher, relative to existing filter cloth, filter paper or canvas etc., there is following effect: (1) culture plate is certainly as rigidity, can realize in a straightforward manner self-supporting (only need simply to fixing bottom it just can uprightly), spatial altitude and size more easily realize, must rely on complicated support frame without the need to such as canvas etc., system of can saving takes up space and cost; (2) surface moisture is even, and micro algae growth is firm, is not easy be subject to environmental influence and come off; (3) tolerable environment factor impact, rotproofness is good, and repeating utilization factor is high, reduces costs; (4) not having the impurity such as fiber is mixed in the algae product of results; (5) water suction and water retention property good, be conducive to micro algae growth breeding increase gradually to nutrient solution consumption requirement.
It is matrix that the core of water-retentivity of the present invention is preferably with molecular sieve, molecular sieve is normally by TO4 (T=Si, P, Al, Ge etc.) tetrahedron form the crystalline inorganic solid with microvoid structure, there is different cages or cavernous structure (aperture is less than 2nm usually).Because molecular sieve can be inner to hole by the molecular adsorption less than its aperture, and the molecular repulsion larger than aperture outside its hole, play the effect of screening molecule, therefore molecular sieve of gaining the name.The practical use of zeolite molecular sieve is very widely, and such as it can be used as sorbent material, ion-exchanger, and especially can be used as petroleum cracking catalyzer, this is that people develop the power with good catalytic activity and selectivity zeolite molecular sieve.Certainly, along with people's deepening continuously to molecular sieve research, its range of application is also further expanded, and such as can be used as battery material, pharmaceutical carrier etc.These characteristics of zeolite molecular sieve depend on the feature of its mechanism and composition aspect, the multi-dimensional nature in such as duct, the size in hole, pore volume, cationic number and site, Si/Al ratio etc., can say that the performance of molecular sieve is the embodiment of the comprehensive action of this several factor.With general conventional solid adsorbent as compared with silica gel, gac, activated alumina etc., molecular sieve has two outstanding features in absorption property, and one is selective adsorption, and another is high-level efficiency absorption.
Due to the pore passage structure of component mainly wetting ability element and multidimensional, molecular sieve has good water absorption energy.Its suction type is mainly physical adsorption, and is mainly stored in the duct of molecular sieve by the water molecules adsorbed, and therefore before and after water suction, molecular screen material volume does not have considerable change.Meanwhile, molecular sieve also has fine performance in water molecules continus convergence, and this is that molecular sieve can make reason that is inner and the homogeneous humidity of surface maintenance in a long time.In addition, molecular sieve also has an important characteristic to be under the assistance of water molecules, can show good transmission performance to metal ion, and the ion of material internal is carried to surface continuously.These features determine the potentiality that molecular sieve has commercial application in semidrying microdisk electrode.
Surface growth formula culture systems water loss provided by the invention is lower, microalgae cell growth is very fast, results are convenient and overall energy consumption is low, and concrete advantage is as follows:
1) what the present invention adopted is semidrying microalgae culture system, after micro-algae is inoculated in the surface of the outside plate of the surface growth formula culture plate of sandwich structure by us, liquid-supplying system is utilized to inject nutritive medium continuously to molecular sieve, glass sand or glass powder core, nutritive medium reaches outer plate surfaces through core interior, grows for frustule.Doing so avoids a large amount of uses of water in traditional bioreactor, decrease the energy consumption of power system; Decrease piping design; Decrease the floor space of unit yield of biomass; Meanwhile, by making micro algae growth concentration greatly improve, also simplify the techniques such as concentrated, centrifugal in results of traditional micro-algae later stage, filtration, improve production efficiency, cut operating costs;
2) in the present invention use light source to shine directly into microalgae cell, and do not need the container through water body and carrying water body, therefore luminous energy decay is less, relatively traditional bioreactor, increase substantially the efficiency of light energy utilization, increased substantially micro algae growth speed and quality, this is key one step of micro-algae Biological Energy Industry simultaneously;
3) conventional tubular bioreactor needs to stir water, algae mixture, to ensure that micro-algae fully grows under the illumination condition of abundance, energy consumption and cost higher.And culture systems involved in the present invention does not need to avoid frustule sedimentation by stirring, realizing frustule and fully grow, thus decrease energy consumption, save cost;
4) relative to other bioreactors, semidrying bioreactor can change micro algae growth environment easily.Two processes of separating the growth (biomass accumulation) of micro-algae and the generation (as grease) of metabolite, because their environmental requirement is not identical, Growth of Cells needs high nitrogen environment, and oil and fat accumulation then needs the stressful environmental such as low nitrogen.Method conventional at present is just progressively converted into nitrogen stress induced environment when being and waiting until that in substratum system, original nitrogenous source is exhausted, and often needs more than 10 days; If growth near plateau, think to enter the oil accumulation stage fast, only have to proceed in low nitrogen or nitrogen-free agar after first gathering frustule again and carry out fat metabolic, very greatly, and energy consumption is high for this workload.In this invention, we can change growing environment at any time according to micro algae growth situation, to realize accumulation or the oil accumulation of micro algae biomass.
5) use the semidrying culture system relatively existing semidrying culture medium material of material of the present invention to compare and there is obvious advantage:
The water-absorbent that 5a) its outside plate of surface growth formula culture plate of sandwich structure is strong with core, water-permeable (owing to having the microtubule that several inside is communicated with in outside plate), thus moisture and other nutritive ingredients can be provided continuously for the cultivation of micro-algae, growth, this is the quick moisturizing in syphonic effect Shi Kong road because water transpiration causes, by good siphon performance, make the humidity on surface homogeneous; And due to molecular sieve, glass sand or glass powder specification homogeneous, realize liquid-flow Quick uniform water-absorbent;
5b) outside plate intensity is high, can realize self-supporting, only needs easy supporting structure support, and the water-absorbent of core molecular sieve, glass sand or glass powder and water-permeable are comparatively strong, make composition board have the advantage of physical strength and biological culture aspect of performance concurrently;
5c) erosion resistance is good, thus after harvesting or pollute after, can sterilizing agent be tolerated, thus ensure that its recycling is good; And
5d) cost is low, molecular sieve, glass sand or the current relative maturity of glass powder materials process for machining and manufacturing, and the starting material that can select can from originally Yuing the waste residue of landfill disposal.
From above-mentioned advantage, this overcomes the technical bottleneck that microdisk electrode mass-producing runs into a great extent---and equipment maximizes and improves space availability ratio obstacle, makes micro-algae Biological Energy Industry have possibility.
6) the surface growth formula culture plate of polylith sandwich structure can be utilized to form the larger culture plate array of area, reduce the manufacturing cost of compound criteria plate.
7) benefit that another one is extra is, not only can reduce the processing cost of waste material such as molecular sieve, glass sand or the glass powder making catalyzer, play Environmental Role, but also carried out environmental improvement at low cost.
In sum, the photo-bioreactor system manufacture and the maintenance cost that the invention solves microdisk electrode are expensive, the problem that space availability ratio is low, production efficiency is low.In the present invention, not only culture systems is relatively cheap, and the utilising efficiency of micro-algae to luminous energy, carbon source and nutrition is high, and the formation speed of secondary metabolite is fast, significantly improves unit occupied area Biomass yield and secondary metabolite productive rate.
Accompanying drawing explanation
Fig. 1 is the schematic diagram of the embodiment of surface growth formula culture plate according to sandwich structure of the present invention;
Fig. 2 is the sectional drawing of the compound criteria plate of Fig. 1;
Fig. 3 is according to the schematic diagram comprising the cultivation unit of the compound criteria plate of Fig. 1 of the present invention;
Fig. 4 is according to the schematic diagram comprising an embodiment of the culture systems of the cultivation unit of Fig. 3 of the present invention; With
Fig. 5 is according to the schematic diagram comprising another embodiment of the culture systems of the cultivation unit of Fig. 3 of the present invention.
Embodiment
It should be noted that the implication that in the present invention, " rigidity " refers to refers to the material relative to canvas, cloth or sponge with larger hardness, include but not limited to the rigid of these building operation such as " permeable ceramic plate, cement plate, potter's clay fire plate, blue bricks plate, common brick plate and watt material plate ", the feature of these materials all can realize self-supporting under the state of vertical direction without special bracing frame and erosion resistance is strong.
In order to illustrate further technical scheme of the present invention and effect thereof, be illustrated below in conjunction with specific embodiment:
The surface growth formula culture plate > of < sandwich structure
Fig. 1 and Fig. 2 shows the surface growth formula culture plate 1 of sandwich structure of the present invention.The surface growth formula culture plate 1 of this sandwich structure comprises outside plate 101 and core 102.As shown in the figure, the core 102 between two outside plates 101 and outside plate 101 of outside forms sandwich type structural, and outside plate 101 can be rigidity pervious material flitch, has good water-permeable, has higher Rigidity and strength simultaneously.The material of outside plate 101 includes but not limited to that permeable ceramic plate, bluestone board, cement plate, potter's clay fire plate, blue bricks plate, common brick plate or watt material plate etc.Core 102 can be the permeable packing material of water suction, includes but not limited to molecular sieve, glass sand, non-woven fabrics, highly dense sponge, cotton, hydroscopic high-molecular resin, super water-absorbent fiber and urethane water-retaining agent.Outside plate 101 is connected to core 102 by embedding the web member 104 of core 102.
Skeleton can be imbedded to support this culture plate and to play the effect guiding nutrient solution in core 102.Skeleton comprises the form of pipeline, network etc.
As shown in Figure 2, nutrient solution 103 enters core 102, and core 102 is saturated mode after absorbing nutrient solution, and nutrient solution oozes out from core surface and enters outside plate 101, and nutrient solution can supply the micro algae growth on outside plate 101 surface through outside plate 101.
The outside plate 101 also substrate material of more available microporosity and additive is made into the many microwell plates of a kind of hard, adopt additive to comprise binding agent, substrate material is molecular sieve, glass sand or green stone etc.; Additive is wherein aluminium stone, or the mixture of aluminium stone, sesbania powder and nitric acid.When adopting molecular sieve to make outside plate 101, the better selection of molecular sieve has
the molecular sieve in the aperture of ~ 2nm, such as molecular sieve beta molecular sieve or ZSM-5 molecular sieve.By mixed-matrix and tackiness agent, additive etc., then the outside plate 101 with multi-pore channel is made in pressing.Wherein the pore size of outside plate 101 affects seepage velocity, and the mode such as particle diameter, pressure size, binder content by Molecular regulator sieve regulates the pore size of outside plate 101.
< cultivates unit >
Fig. 3 is the schematic diagram cultivating unit 100.Cultivate surface growth formula culture plate 1, stationary installation 2 that unit can comprise sandwich structure, described stationary installation 2 can comprise the assembly 21 and 22 that is relatively fixed, being formed between fixation kit 21 and 22 can the hole 23 of surface growth formula culture plate 1 of retaining clip core structure, and culture plate 1 can shift out this stationary installation 2 from top.Cultivate the array that unit 100 can comprise the surface growth formula culture plate 1 of multiple sandwich structure, it forms larger plane culture plate assembly.
< surface growth formula culture systems embodiment 1>
Composition graphs 4 shows an embodiment of culture systems of the present invention.
The surface growth formula culture systems 1000a of Fig. 4 can comprise multiple cultivation unit 100 as shown in Figure 3 and be arranged on and cultivates light supply apparatus 8 between unit 100, and provides the nutrient solution circulation device 9a of nutrient solution for cultivating unit 100.
Multiple cultivation unit 100 in Fig. 4 are set to be parallel to each other and are spaced a distance.Between every two cultivation unit 100, at least one light supply apparatus 8 is set.Light supply apparatus can be two-sided light source, irradiates the cultivation unit of both sides.Light supply apparatus 8 can be also single surface light source as required.
The surface growth formula culture systems 1000a of Fig. 4 comprises nutrient solution circulation device 9a, this nutrient solution circulation device 9a comprise cultivate liquid pool 6, recycle pump 7, be arranged on described sandwich structure surface growth formula culture plate 1 below nutrient solution retrieving arrangement 3 and the liquid feed device that is arranged on above described culture plate 1.The nutrient solution of collection for collecting the nutrient solution not absorbed by culture plate 1 or ooze out from culture plate 1, and is back to cultivation liquid pool 6 by nutrient solution retrieving arrangement 3.When culture systems 1000a works, recycle pump 7 starts, by the nutrient solution cultivated in liquid pool 6 by Cemented filling to liquid feed device 4, liquid feed device 4 is arranged on the top of the surface growth formula culture plate 1 of sandwich structure, liquid feed device 4 can have the outlet such as hole, gap, makes nutrient solution enter the surface growth formula culture plate 1 of sandwich structure with the form such as water clock, seepage; And surface growth formula culture plate 1 bottom of sandwich structure contacts with the nutrient solution in nutrient solution retrieving arrangement 3, nutrient solution infiltrates the region, surface growth formula culture plate 1 one section, bottom of sandwich structure by wicking action, makes culture plate 1 be in leather hard.
The low side lateral margin of the surface growth formula culture plate 1 of sandwich structure is salable in nutrient solution retrieving arrangement 3, and the top lateral margin of the surface growth formula culture plate 1 of sandwich structure is salable or covered in liquid feed device 4.As replacement, liquid feed device 4 at the spaced apart segment distance of surface growth formula culture plate 1 of vertical direction and sandwich structure, can spray, drop to the surface growth formula culture plate 1 of sandwich structure from liquid feed device 4 to make nutrient solution.
Nutrient solution circulation device 9a also can comprise carbonic acid gas and be mixed into device, for being mixed in nutrient solution by carbonic acid gas.
< surface growth formula culture systems embodiment 2>
Composition graphs 3 and Fig. 5 illustrate another culture systems of the present invention.Fig. 5 is according to the schematic diagram comprising another embodiment 1000b of the surface growth formula culture systems of the cultivation unit of Fig. 3 of the present invention.Similar with the embodiment shown in Fig. 4, culture systems 1000b also can comprise the cultivation unit 100 of one or more Fig. 3.Particularly, culture systems 1000b can comprise cultivation unit 100, nutrient solution retrieving arrangement 3, liquid feed device 4, cultivate liquid pool 6, pressure-pot 11, air compressing source of the gas 12, liquidometer 13, stopping valve 14, stopping valve 15, reducing valve 16, vent valve 17 and tensimeter 18.
Shown in Fig. 5, surface growth formula culture systems 1000b comprises nutrient solution circulation device 9b, optional employing air compressing mode in described nutrient solution circulation device 9b.This air compressing mode equipment therefor is this area other pressure pump replacement device optional, optional but be not limited to comprise pressure-pot 11, air compressing source of the gas 12, liquidometer 13 and mating valve, preferred stopping valve 14,15, reducing valve 16 etc.By by pressure-pot 11 lay in enough nutrient solutions condition under, its upstream valve 15 in circulation device 9b is closed, stopping valve 14 is opened, nutrient solution in pressure-pot 11 is made to be delivered to liquid feed device 4 in circulation device 9b by starting air compressing source of the gas 12, tensimeter 18 can be selected to monitor, liquid feed device 4 is with spray, the mode of water clock or seepage provides nutrient solution to the surface growth formula culture plate 1 of sandwich structure, thus make the surface growth formula culture plate 1 infiltrating whole sandwich structure containing the nutrient solution cultivating desired nutritional material, nutrient solution retrieving arrangement 3 is positioned at the below of the surface growth formula culture plate of sandwich structure, do not flow to nutrient solution retrieving arrangement 3 by the nutrient solution that the surface growth formula culture plate 1 of sandwich structure absorbs, nutrient solution in nutrient solution retrieving arrangement 3 is flow to by back of pipeline and cultivates liquid pool 6.
The operation > of < surface growth formula culture systems
Surface growth formula culture systems 1000a (Fig. 4) of the present invention can be operated to cultivate micro-algae, can comprise the following steps.
By nutrient solution circulation device 9a, liquid feed device 4 is had and comprise CO
2the nutrient solution of microvesicle, liquid feed device 4 provides nutrient solution in the mode of spray, water clock or seepage to the surface growth formula culture plate 1 of sandwich structure;
The surface growth formula culture plate 1 of sandwich structure is kept in touch with the nutrient solution in nutrient solution retrieving arrangement 3, thus makes the nutrient solution containing CO2 microvesicle infiltrate the surface growth formula culture plate 1 of whole sandwich structure;
In the micro-algae of surface growth formula culture plate 1 surface seeding of sandwich structure;
Described light supply apparatus 8 is utilized to irradiate described culture plate, and the ambient moisture arranged around culture systems 1000a and temperature, make micro algae growth.
The surface growth formula culture systems 1000b (Fig. 5) of another embodiment of the present invention can be operated to cultivate micro-algae, can comprise the following steps:
1) vent valve 17 is opened, and stopping valve 14 and stopping valve 15 are closed, and reducing valve 16 pressure is adjusted to 0MPa (reducing valve 16 is adjustable within the scope of 0-1.0MPa).
2) nutrient solution of certain volume amount is added at nutrient solution pond for recovering 6.
3) open stopping valve 15, nutrient solution from nutrient solution pond for recovering 6 feed pressure tank 11, below the line entry that liquid level to stopping valve 14 is connected.
4) close stopping valve 15 and vent valve 17, open stopping valve 14, the pressure of adjustment reducing valve 16, the nutrient solution in pressure-pot 11 can flow into liquid feed device 4 gradually, for culture plate 1 provide micro-algae grow required nutrient solution.(wherein, according to the pressure that micro algae growth situation on culture plate 1 and tensimeter 18 show, by the pressure adjusting of reducing valve 16 to suitable value.)
5) nutrient solution flowing through nutrient solution retrieving arrangement 3 turns back in nutrient solution pond for recovering 6, carries out Real-Time Monitoring to the composition in nutrient solution pond for recovering 6, if composition does not reach fostering requirement, supplements corresponding nutrient solution composition in time.
6) be provided with the liquidometer 13 of low Level Detection in pressure-pot 11, after liquid level is lower than the liquid level set, opened by vent valve 17, stopping valve 15 is opened, and stopping valve 14 is closed, and nutrient solution is from nutrient solution pond for recovering 6 feed pressure tank 11.
Repeating step 3)-step 5) process, realize algae non-interruptible supply nutrient solution micro-on culture plate.
< microdisk electrode embodiment >
The surface growth formula culture plate of embodiment 1 sandwich structure of the present invention and the preparation of contrast
First, zeolite molecular sieve is arranged between water-permeable pottery outside plate as core and forms the surface growth formula culture plate of sandwich structure, and zeolite molecular sieve can formation platy structure compressed together in advance, to be arranged between outside plate.The surface growth formula culture plate of described sandwich structure is of a size of 1 meter * 2 meters * 3 centimetres, wherein pottery outer plate thickness 0.5 centimetre.
Contrast is set: contrast canvas, the surface growth formula culture plate of size and described sandwich structure is suitable.
Embodiment 2 water-intake rate compares
Table 1 water-intake rate compares
From table 1, the surface growth formula culture plate water-intake rate of sandwich structure of the present invention is respectively apparently higher than contrast.
Embodiment 3 culture experiment
3.1. nutrient solution composition
Consist of example with the nutrient solution that chlorella is cultivated, see the following form 2.
The nutrient solution composition that table 2 chlorella is cultivated
| Component | Usage quantity | Mother liquor |
| NaNO 3 | 1m L/L | 25g/100ml dH 2O |
| K 2HPO 4 | 1m L/L | 7.5g/100ml dH 2O |
| MgSO 4·7H 2O | 1m L/L | 7.5g/100ml dH 2O |
| CaCl 2·2H 2O | 1m L/L | 2.5g/100ml dH 2O |
| KH 2PO 4 | 1m L/L | 17.5g/100ml dH 2O |
| NaCl | 1m L/L | 2.5g/100ml dH 2O |
| Na 2CO 3 | 1m L/L | 0.2g/100ml dH 2O |
| FeCl 3·6H 2O | 1m L/L | 0.05g/100ml dH 2O |
| EDTA-Fe | 1m L/L | |
| Trace metal solutions | 1m L/L | |
| Soil extract | 40m L/L |
The formula of table 3 trace metal solutions
| Component | Mother liquor |
| H 3BO 3 | 2.86g/100m L dH 2O |
| MnCl 2·4H 2O | 1.86g/100m L dH 2O |
| ZnSO 4·7H 2O | 0.22g/100m L dH 2O |
| Na 2MO 4·2H 2O | 0.39g/100m L dH 2O |
| CuSO 4·5H 2O | 0.08g/100m L dH 2O |
| Co(NO 3) 2·6H 2O | 0.05g/100m L dH 2O |
In addition, in nutrient solution, the preparation method of EDTA-Fe is: get 4.1mL concentrated hydrochloric acid distilled water diluting to 50mL, make 1N HCl; Take 0.93g EDTA-Na
2and be dissolved in 500mL distilled water, make 0.1N EDTA-Na
2solution; Take FeCl
36H
2o 0.90g is also dissolved in the above-mentioned 1N HCl of 10mL, then with 10ml 0.1N EDTA-Na
2solution mixes, and adds distilled water diluting to 1000m L.Soil extract liquid making method is: get the garden soil 200g not executing overfertilization and be placed in beaker or triangular flask, add distilled water 1000m L, bottleneck porous plug seals, boiling water heats 3 hours in a water bath, and cooling, precipitates 24 hours, this process carries out 3 times continuously, then filter, get supernatant liquor, save backup in 4 DEG C of refrigerators after sterilizing in high-pressure sterilizing pot.
According to the formula of as above table 2 and table 3, chlorella nutrient solution used is cultivated in preparation.Be stored in container stand-by.
3.2. the assembling of culture systems of the present invention and control board
According to the culture systems shown in Fig. 4, in the gap 23 between the relative fixation kit 21 and 22 respectively the surface growth formula culture plate of sandwich structure, canvas being individually fixed in stationary installation 2.
the operation of 3.3 culture systems
State with reference to Fig. 4, surface growth formula culture systems 1000a of the present invention be can be used for cultivating micro-algae, can comprise the following steps:
-make liquid feed device 4 have nutrient solution by nutrient solution circulation device 9a, liquid feed device 4 provides nutrient solution in the mode of spray, water clock or seepage to porous plate;
-make the nutrient solution in liquid feed device 4 instill compound criteria plate 1;
-on culture plate 1, inoculate micro-algae;
Described light supply apparatus 8 is utilized to irradiate culture plate 1, and the ambient moisture arranged around culture systems 1000a and temperature, temperature is generally 20-35 DEG C, and humidity, in the scope of 50-80%, makes micro algae growth.
3.4. culture Comparative result
Cultivate 24, after 48 and 72 hours, respectively from harvesting microalgae culture the surface growth formula culture plate of the sandwich structure of the present invention of same homalographic, contrast canvas, after 70 DEG C of drying and dehydratings, take dry weight and carry out dry biomass weight ratio comparatively.
Table 4 cultivation results compares
From table 4, micro-algae dry biomass the surface growth formula culture plate of sandwich structure of the present invention output apparently higher than contrast canvas.
Claims (21)
1. a surface growth formula culture plate for sandwich structure, is characterized in that, comprises outside plate and the core being positioned at outside plate,
Wherein, this outside plate is rigidity pervious material flitch, and
Wherein, this core is water suction lock water material, and this core is located between this both sides outside plate.
2. the surface growth formula culture plate of sandwich structure according to claim 1, is characterized in that, described core is molecular sieve, glass sand, non-woven fabrics, highly dense sponge, cotton, hydroscopic high-molecular resin, super water-absorbent fiber or urethane water-retaining agent.
3. the surface growth formula culture plate of sandwich structure according to claim 2, is characterized in that, described outside plate is provided with the web member embedding this core, and this core is fixed between this both sides outside plate.
4. the surface growth formula culture plate of the sandwich structure as described in claim 1 or 3, is characterized in that, described outside plate is permeable ceramic plate, green stone, cement plate, potter's clay fire plate, blue bricks plate, common brick plate or watt material plate.
5. the surface growth formula culture plate of the sandwich structure as described in claim 1 or 3, is characterized in that, described outside plate is adopt the matrix of microporosity and the hard porous plate of tackiness agent pressing, and described substrate material is molecular sieve, tackiness agent comprises aluminium stone.
6. the surface growth formula culture plate of the sandwich structure as described in claim 1 or 3, is characterized in that, imbeds skeleton to support this culture plate and for guiding nutrient solution in described core.
7. cultivate a unit, it is characterized in that, this cultivation unit comprises surface growth formula culture plate and the stationary installation thereof of the sandwich structure according to any one of claim 1-6.
8. cultivate unit as claimed in claim 7, it is characterized in that, this cultivation unit comprises the surface growth formula culture plate of multiple described sandwich structure, and the surface growth formula culture plate of the plurality of sandwich structure forms single writing board shape.
9. a surface growth formula culture systems, is characterized in that,
This culture systems comprises:
At least one cultivates unit as claimed in claim 7 or 8, and
Nutrient solution circulation device.
10. surface growth formula culture systems as claimed in claim 9, it is characterized in that, described cultivation unit is at least two, is provided with light supply apparatus between adjacent cultivation unit, and this light supply apparatus and described cultivation unit are arranged abreast; Or the parallel placement of described each cultivation unit forms array, and a light supply apparatus is located at the side of this array, with respectively this cultivation unit is vertical.
11. surface growth formula culture systems as claimed in claim 10, it is characterized in that, described light source is natural light or source of artificial light, and described source of artificial light is two-sided light source or single surface light source.
12. surface growth formula culture systems as described in any one of claim 9-11, it is characterized in that, described nutrient solution circulation device comprises cultivation liquid pool, recycle pump, liquid feed device and nutrient solution retrieving arrangement, nutrient solution in described cultivation liquid pool is delivered to described liquid feed device by recycle pump, described liquid feed device is located at above the surface growth formula culture plate of described sandwich structure, and with spray, the mode of water clock or seepage provides nutrient solution to the surface growth formula culture plate of sandwich structure, the below of the surface growth formula culture plate of described sandwich structure is located at by described nutrient solution retrieving arrangement, oozed out or unabsorbed nutrient solution by the surface growth formula culture plate of sandwich structure in order to collect, and be back to cultivation liquid pool by pipeline.
13. surface growth formula culture systems as claimed in claim 12, is characterized in that, what described nutrient solution circulation device also comprised nutritive substance needed for cultivation is mixed into device.
14. surface growth formula culture systems as claimed in claim 12, it is characterized in that, the surface growth formula culture plate of described sandwich structure contacts with the nutrient solution in nutrient solution retrieving arrangement, makes the nutrient solution in nutrient solution retrieving arrangement be infiltrated the subregion of the bottom of the surface growth formula culture plate of this sandwich structure by wicking action.
15. surface growth formula culture systems as described in any one of claim 9-11, it is characterized in that, described nutrient solution circulation device comprises cultivation liquid pool, pressure-pot, air compressing source of the gas, liquid feed device and nutrient solution retrieving arrangement, described pressure-pot fluid inlet is connected with described cultivation liquid pool, for temporary a certain amount of nutrient solution, this air compressing source of the gas is connected with to supercharging in pressure-pot with this pressure-pot inlet mouth, nutrient solution in pressure-pot is extruded and is delivered to respectively this liquid feed device, described liquid feed device is located at above the surface growth formula culture plate of described sandwich structure, and with spray, the mode of water clock or seepage provides nutrient solution to the surface growth formula culture plate of sandwich structure, the below of the surface growth formula culture plate of described sandwich structure is located at by described nutrient solution retrieving arrangement, oozed out or unabsorbed nutrient solution by the surface growth formula culture plate of sandwich structure in order to collect, and be back to cultivation liquid pool by pipeline.
16. surface growth formula culture systems as claimed in claim 15, it is characterized in that, described pressure-pot connects a liquidometer, and the pipeline that this air compressing source of the gas is connected to this pressure-pot is provided with reducing valve, stopping valve; The pipeline that this pressure-pot is connected to liquid feed device is provided with tensimeter; The venting port of this pressure-pot is provided with stopping valve; Pipeline between this cultivation liquid pool and this pressure-pot is provided with stopping valve.
17. surface growth formula culture systems as claimed in claim 9, is characterized in that, the outside surface of the surface growth formula culture plate of the sandwich structure of described cultivation unit is for adhering to micro-algae algae kind.
18. 1 kinds utilize surface growth formula culture systems to cultivate hydrobiological method, it is characterized in that, this culture systems is the surface growth formula culture systems such as according to any one of claim 9-17, and the method comprises the steps:
A) by nutrient solution circulation device, the nutrient solution cultivated in liquid pool is delivered to the surface growth formula culture plate of the sandwich structure of each cultivation unit, thus makes nutrient solution infiltrate the surface growth formula culture plate of whole sandwich structure;
B) the surface growth formula culture plate surface seeding hydrobiont of described sandwich structure;
C) ambient moisture and temperature are set, under the photon intensity that applicable hydrobiont grows, hydrobiont are grown.
19. methods as claimed in claim 18, is characterized in that, step a) in, the nutrient solution composition in liquid pool is cultivated in monitoring, supplements nutrient solution in time.
20. methods as claimed in claim 18, it is characterized in that, described hydrobiont is micro-algae.
21. methods as claimed in claim 18, is characterized in that, described micro-algae comprises chlorella, spirulina, green alga, grid algae, Isochrysis galbana, micro-plan ball algae or blood cell algae.
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Application publication date: 20150204 |