CN103820318A - Optical organism culture device - Google Patents
Optical organism culture device Download PDFInfo
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- CN103820318A CN103820318A CN201410077552.2A CN201410077552A CN103820318A CN 103820318 A CN103820318 A CN 103820318A CN 201410077552 A CN201410077552 A CN 201410077552A CN 103820318 A CN103820318 A CN 103820318A
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
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Abstract
The invention relates to an optical organism culture device, which comprises one or more supports and a plurality of vectors, wherein the vectors are spaced, and can adsorb optical organism cells, nutrient liquid and optical organism culture liquid, and each vector is suspended from one support. The optical organism culture device has a good light splitting effect, specifically, optical organisms are cultivated on the vectors suspended on the supports, as a result, the defect that optical organisms at the lower part of a tank body receives deficient light when the optical organisms are cultivated in the tank body can be overcome, and the optical organisms located on the vectors receive sufficient light. In addition, the distance and angle of two or more vectors arranged at intervals are adjustable, mutual light shielding of the two or more vectors can be reduced, and further the optical organisms on the vectors receive sufficient light. In a word, the optical organism culture device provided by the invention has a better light splitting effect, and accordingly, the yield of the optical organisms can be improved. Moreover, the optical organism culture device is simple in structure and low in cost, and can be applied to large-scale cultivation, and as a result, the overall yield of the optical organisms is improved.
Description
Technical field
The present invention relates to a kind of photo bio cultivation apparatus.
Background technology
Photosynthetic microorganism or vegetable cell, can utilize the form inorganicss such as sun power, water and simple mineral substance synthesis of organic substance and hydrogen, methane.Micro-algae is exactly one of them Typical Representative, and it is a class individual small photosynthetic autotrophs/type of supporting of holding concurrently, unicellular/simple multicellular organism, has the features such as widely distributed, of a great variety, photosynthetic efficiency is high, fast growth, strong adaptability.The annual fixing CO of micro-algae
2account for 40% of global net photosynthesis output, add it and be rich in the high value oxidation-resistance natural pigments such as lipid, hydro carbons, albumen, soluble polysaccharide, therefore micro-algae receives much attention in fields such as environmental protection, the energy and health cares.
Take " large water body is cultivated ", as main, its culture systems mainly comprises open raceway pond and sealing/semi-enclosed reactor to existing micro-algae training method.Raceway pond mostly is oval or circular shallow pond, and in pond, mostly to be 20-30cm dark for algae liquid, by cylinder shape stirring rake, algae liquid is carried out to prolonged agitation, thereby realizes circulating of whole pond algae liquid, so that microalgae cell and each nutrition are evenly distributed in algae liquid.But the cultured output of raceway pond is always not ideal enough, on the one hand due to along with microalgae cell growth, algae liquid turbidity raises gradually, sunlight decay to zero after taking in several centimetres, algae liquid top layer, only top layer liquid level skim microalgae cell visible ray carries out photosynthesis, because the mixed effect of algae liquid in the vertical direction is poor, the sensitive surface of unit volume algae liquid is less on the other hand, and light utilising efficiency is low.Sealing/semi-enclosed reactor is pipe, post, tabular more, and height can reach more than 2 meters.The light utilising efficiency of reactor increases with increase of its height, but meanwhile reactor middle part and bottom institute liquid body (algae liquid) pressure also increase, and cause reactor manufacturing cost and air feed energy consumption significantly to improve.In addition, reactor sensitive surface cannot be made corresponding adjustment with the variation of extraneous lamp, and height for reactor is subject to its manufacture material (particularly sensitive surface material) to possess the conditionality of better light transmission and higher-strength simultaneously, also makes the room for promotion of reactor light utilising efficiency be restricted.
U.S. Pat 2011/0217764A1 discloses one makes frustule be attached to rope surface, and make the rope being wound around thereon constantly be exposed in nutritive medium and sunlight by roller structure, the culture apparatus that frustule on rope is grown, it has abandoned traditional " large water body culture method " take liquid as culture carrier, and adopted " plate method ", frustule is mainly grown on carrier.Above-mentioned culture apparatus space availability ratio deficiency, " light splitting " effect is limited, and cannot regulate light splitting (be equivalent to cell and be subject to light) with the demand of external environment (light intensity) variation and the different growth phases of frustule.
Document Growing microalgae as aquaculture feeds on twin-layers:a novel solid-state photobioreactor and Attached cultivation technology of microalgae for efficient biomass feedstock production disclose " bilayer " micro-algae immobilization cultivating system, and this system is mainly made up of " resource " layer, " matrix " layer and the recycle system." resource " layer and " matrix " layer can also vertically arrange by flakiness shape, the recycle system is constantly to " resource " layer supplying nutrition liquid, " matrix " layer one side adhered to microalgae cell, opposite side is directly attached to " resource " layer both sides, so that frustule attached to it obtains nutritive substance and grows.Above-mentioned micro-algae immobilization cultivating system, its " bilayer " structure inessential, and frustule on " matrix " layer really fixes with " matrix " layer, in breeding process frustule be easy to " matrix " pull-up from.In addition, this system cannot regulate light splitting (be equivalent to cell and be subject to light) with the demand of external environment (light intensity) variation and the different growth phases of frustule, and each cultivation face in system (" resource " layer and " matrix " layer) is in the light mutually.Meanwhile, the disclosed device of document Attached cultivation technology of microalgae for efficient biomass feedstock production is hedged off from the outer world, so that frustule is containing CO
2atmosphere surrounding in grow.But CO in light intensity, temperature, air in physical environment
2dividing potential drop degree of controllability is low, and construction glasshouse (so that closed environment in document to be provided) cost input is high, amplifies application therefore this device is not easy to industrialization.
Summary of the invention
The object of the present invention is to provide the photo bio cultivation apparatus that a kind of spectrophotometric result is good.
For achieving the above object, provide a kind of photo bio cultivation apparatus, comprising: at least one resting support; And the carrier of at least two isolated, adsorbable photo bio cells, nutritive medium and photo bio culturing liquids, each carrier hangs on resting support.
According to the present invention, resting support has the first strut member, each carrier or be suspended to the first strut member and/or suspend in midair slidably along the first strut member in the rotating mode of the axis perpendicular to the first strut member.
According to the present invention, carrier is sheet shaped piece, by guiding piece, all sheet shaped pieces on same the first strut member are together in series, and guiding piece and the first strut member is spaced apart from each other.
According to the present invention, carrier is the rod-like element of suspending in midair slidably along the first strut member.
According to the present invention, carrier is made up of one or more materials in glass fibre, nylon, cotton, fiber crops, carbon fiber, synthon, sponge, plastic foam, metal, synthetic plastics.
According to the present invention, carrier comprises the covering part of base portion and covering base portion, and covering part is made up of the sorbing material of adsorbable photo bio cell, nutritive medium and photo bio culturing liquid.
According to the present invention, carrier is concave/convex-shaped structure or the structure that is provided with through hole.
According to the present invention, also comprise: for carrier provides the circulation device of nutritive medium or photo bio culturing liquid.
According to the present invention, circulation device comprises: be arranged at the cloth liquid part on resting support, the liquid outlet that cloth liquid part comprises fluid inlet and arranges towards carrier; And the infusion set being communicated with the fluid inlet fluid of cloth liquid part.
According to the present invention, resting support comprises: two compartment of terrains and the supporting bar being arranged in parallel; Two ends are connected in respectively the first strut member of two supporting bars movably, and wherein carrier hangs on the first strut member; Circulation device comprises: drive the first strut member to come and go mobile drive element along supporting bar.
According to the present invention, it is characterized in that, be provided with at interval multiple resting supports, and the orientation of multiple resting supports is perpendicular to the orientation of the carrier on same resting support.
Than prior art, beneficial effect of the present invention is:
1. on the carrier hanging on resting support, cultivate photo bio, can avoid cultivating and making bottom photo bio be subject to the shortcoming of light deficiency at Chi Tizhong, the photo bio that makes to be positioned on carrier is fully subject to light.In addition, at least two carriers that each interval is arranged, can reduce the mutual shading between carrier, further make the photo bio on carrier fully be subject to light.To sum up, photo bio cultivation apparatus of the present invention has good spectrophotometric result, can improve thus the output of photo bio.Further, photo bio cultivation apparatus of the present invention is simple in structure, cost is low, and high compared with the high three-dimensional space availability ratio of expanding space because having, so can, by this device for large-scale farming, improve thus the overall yield of photo bio.
2. carrier is suspended on the first strut member in the rotating mode of axis of the first strut member perpendicular to resting support, make carrier can regulate according to direction of illumination and photo bio carrier setting party from the demand in different growth phases to, be that angle between carrier is adjustable, improve thus spectrophotometric result, further avoid shading mutually between carrier, thereby can cultivate more carrier under the prerequisite that meets illumination demand.Particularly, for example, when sunlight is light source, can pass through rotation vector, make carrier surface evenly be subject to light, improve thus spectrophotometric result, thereby improve photo bio productive rate.Again for example, when carrier is sheet shaped piece, can drive sheet shaped piece to rotate by being connected in the guiding piece of carrier, so that the long-pending surface of maximized surface that has of each sheet shaped piece keeps being subject to all the time light (without mutually blocking) completely, make thus the photo bio cell of this surface adsorption can fully be subject to light.
3. carrier is suspended on the first strut member slidably, make carrier can regulate according to direction of illumination and photo bio the spacing of carrier from the demand in different growth phases,, spacing between carrier is adjustable, improve thus spectrophotometric result, further avoid the mutual shading between carrier, and met under the prerequisite of illumination requirement, can cultivate more photo bio cell.Particularly, can, according to for example intensity of illumination, direction of illumination and the demand of different photo bio to light, regulate the suspension spacing of carrier, can improve thus the utilization ratio of illumination, under the prerequisite meeting the demands, cultivate more photo bio.
4. by the circulation device of nutritive medium or photo bio culturing liquid (nutritive medium that contains photo bio cell) is provided for carrier, can be carrier supply nutritive medium or in supply nutritive medium, introduce new photo bio cell, in illumination abundance, guarantee that thus photo bio cell grows quickly.For example, by driving the first strut member to move downward, can be by carrier part ground or immerse fully carrier below and be equipped with the Chi Tizhong of nutritive medium or photo bio culturing liquid (nutritive medium that contains photo bio cell), thus for carrier supply nutritive medium or supply nutritive medium time, introduce new photo bio cell.Again for example, by being arranged at the cloth liquid part on resting support, to carrier spraying nutrient solution or photo bio culturing liquid, thus for carrier supply nutritive medium or supply nutritive medium time, introduce new photo bio cell.
Accompanying drawing explanation
Fig. 1 is the schematic diagram of first embodiment of photo bio cultivation apparatus of the present invention;
Fig. 2 is the schematic diagram that photo bio cultivation apparatus illustrated in fig. 1 adds guiding piece 6;
Fig. 3 is the schematic diagram that the carrier in photo bio cultivation apparatus illustrated in fig. 1 is located according to a direction of illumination;
Fig. 4 is the schematic diagram that the carrier in photo bio cultivation apparatus illustrated in fig. 1 is located according to another direction of illumination;
Fig. 5 is the schematic diagram of second embodiment of photo bio cultivation apparatus of the present invention;
Fig. 6 is the schematic diagram that adds cloth liquid part and guiding piece in the photo bio cultivation apparatus shown in Fig. 5;
Fig. 7 is the schematic diagram of the 3rd embodiment of the present invention;
Fig. 8 is the schematic diagram of the 4th embodiment of the present invention;
Fig. 9 is the first strut member shown in Fig. 8 and the partial schematic diagram of carrier;
Figure 10 is the partial schematic diagram of the 5th embodiment of the present invention.
Embodiment
Below in conjunction with accompanying drawing, the specific embodiment of the invention is described.
With reference to Fig. 1, first embodiment of photo bio cultivation apparatus of the present invention, comprising: at least one resting support 2; And the carrier 4 of at least two isolated, adsorbable photo bio cells, nutritive medium and photo bio culturing liquids, each carrier 4 hangs on resting support 2.
Wherein, carrier 4 preferably, flexible high by water-retentivity, durable material is made, and it is slight to cultivated photo bio cell nontoxicity or toxicity.Carrier 4 is made up of one or more materials in glass fibre, nylon, cotton, fiber crops, carbon fiber, synthon, sponge, plastic foam, metal, synthetic plastics alternatively, to adsorb photo bio cell, nutritive medium and photo bio culturing liquid.Or carrier 4 comprises the covering part of base portion and covering base portion alternatively, the base portion material with certain degree of hardness of serving as reasons is made, and covering part is by adsorbable photo bio cell, the sorbing material of nutritive medium and photo bio culturing liquid is made, particularly, sorbing material is glass fibre, nylon, cotton, fiber crops, carbon fiber, synthon, sponge, plastic foam, metal, one or more materials in synthetic plastics, thus covering part is covered on base portion to form adsorbable photo bio cell, nutritive medium and photo bio culturing liquid, there is certain degree of hardness and in the time hanging, can keep the carrier 4 flattening.In the present embodiment, carrier 4 is made up of white cheese cloth, the rectangle sheet shaped piece of long 1.5 meters, wide 0.3 meter, thick approximately 0.001 meter, and the sticking plaster (not shown) binding with 0.3 meter of length, 0.05 meter of diameter with line respectively of two narrow limits, to maintain the flattened state of carrier.
Thus, on the carrier 4 hanging on resting support 2, cultivate photo bio, can avoid cultivating and making body bottom, pond photo bio be subject to the shortcoming of light deficiency at Chi Tizhong, the photo bio that makes to be positioned on carrier 4 is fully subject to light.In addition, at least two carriers 4 that each interval is arranged, can reduce the mutual shading between carrier 4, further make the photo bio on carrier 4 fully be subject to light.To sum up, photo bio cultivation apparatus of the present invention has good spectrophotometric result, can improve thus the output of photo bio.Further, photo bio cultivation apparatus of the present invention is simple in structure, cost is low, and high compared with the high three-dimensional space availability ratio of expanding space because having, thus by this device for large-scale farming, improve thus the overall yield of photo bio.
With further reference to Fig. 1, in the present embodiment, be provided with at interval 4 resting supports 2, and the orientation of 4 resting supports 2 is perpendicular to the orientation of the carrier on same resting support, preferably, and 4 equally spaced from one another opening of resting support 2, and spacing is 1 meter.In addition, each resting support 2 is provided with two compartment of terrains and the supporting bar 7 being arranged in parallel and two ends and is connected in movably respectively the first strut member 3 of two described supporting bars 7.Wherein carrier 4 is sheet shaped piece, and it is suspended on the first strut member 3 in the rotating mode of the axis perpendicular to the first strut member 3, the mid point on a narrow limit of carrier 4 and the first strut member 3 are fixing rotationally, and carrier 4 can rotate along the vertical imaginary axis of crossing point of fixity, and on same the first strut member 3, the spacing of adjacent two carriers 4 is 0.5 meter.Can understand, the first strut member 3 is connected in two supporting bars 7, and the mode prop carrier 4 suspending in midair for carrier 4.Alternatively, the first strut member 3 can be rod member or the flexible strand of rigidity.Wherein, supporting bar 7 has good intensity to realize firm supporting.Certainly, the magnitude setting of resting support 2 is not limited to this, selects the resting support 2 of suitable number in the situation that can guaranteeing that every day, carrier 4 was allowed by light and space fully.
With reference to Fig. 2, in the present embodiment, can drive carrier 4 to rotate by adding guiding piece 6.Alternatively, guiding piece 6 can be rod member or the flexible strand of rigidity.Particularly, by guiding piece 6, all sheet shaped pieces on same the first strut member 3 (being carrier 4) are together in series, and guiding piece 6 and the first strut member 3 are spaced apart from each other.Should be appreciated that because guiding piece 6 can drive carrier 4 and rotate, thus guiding piece 6 and the first strut member 3 arrange abreast and not with the first strut member 3 in same perpendicular, this perpendicular vertical level.In the present embodiment, in two narrow limits of the each carrier 4 on same the first strut member 3, the summit, the same side on the narrow limit of close the first strut member 3 is connected with guiding piece 6, regulate carrier 4 angles so that unified, the unified rotation of controlling carrier 4, can realize the adjusting of carrier 4 angles (sensitive surface direction) thus by pulling guiding piece 6.Alternatively, guiding piece 6 is made up of approximately 0.01 meter of rope made of hemp.Certainly, guiding piece 6 is not limited to this with form, mode of connection and the position of carrier 1, as long as can realize the rotation of controlling carrier 4 by guiding piece 6.Alternatively, multiple guiding pieces 6 also can be set, make to drive carrier 4 to rotate convenient laborsaving.
Carrier is suspended on the first strut member 3 in the rotating mode of axis of the first strut member 3 perpendicular to resting support 2, make carrier 4 to regulate the setting party of carrier to (regulating the angle between carrier) from the demand in different growth phases according to direction of illumination (with reference to Fig. 3 and Fig. 4), intensity of illumination and photo bio, improve thus spectrophotometric result, and under the prerequisite that meets illumination requirement, can hang more carrier 4 to improve output.Particularly, for example, when sunlight is light source, can pass through rotation vector, make carrier surface evenly be subject to light, improve thus spectrophotometric result, thereby improve photo bio productive rate.Again for example, when carrier is sheet shaped piece, can drive sheet shaped piece to rotate by being connected in the guiding piece of carrier, so that the long-pending surface of maximized surface that has of sheet shaped piece is fully subject to solar radiation, does not mutually block, make thus the photo bio cell of this surface adsorption can obtain suitable light intensity, thereby improve overall yield.
In addition, in the present embodiment, be also included as carrier 4 circulation device of nutritive medium or photo bio culturing liquid (nutritive medium that contains photo bio cell) is provided, circulation device comprises that driving the first strut member 3 comes and goes mobile drive element and is positioned at the pond body 1 of carrier 4 belows along supporting bar 7.Alternatively, pond body 1 is brick square shallow pond, lined with PVC material, equal 5 meters of length and width, high 0.6 meter.In addition, in pond body 1, fill the nutritive medium or the photo bio culturing liquid that are rich in micro algae growth desired nutritional material, and nutritive medium or the dark 0.1-0.2 rice of photo bio culturing liquid.And supporting bar can be positioned at pond body 1 outside and is for example supported on ground, certainly, also can be positioned on the edge of pond body 1 inside or pond body 1.Certainly, the structure of pond body 1 is not limited to form shown in Fig. 1, can establish monolithic on multiple respectively corresponding first strut members or the pond body 1 of several carriers, and the nutritive medium or photo bio culturing liquid and the sterilizing agent that are rich in micro algae growth desired nutritional material are set respectively in the body of each pond.Thus, can, according to Expenditure Levels Additional nutrient solution or photo bio culturing liquids such as nutritive substances in the body of different ponds, better to control the cultivation of photo bio, boost productivity and benefit.Certainly, also can the nutritive medium in the body of each pond be collected to rear unification by pipeline supplements.
In addition, circulation device is to determine according to the absorption property of the concrete kind of photo bio and carrier 4 is common for carrier 4 provides nutritive medium or photo bio culturing liquid (containing nutritive medium and photo bio cell), being originally in embodiment, in pond body 1, holding nutritive medium or photo bio culturing liquid is to determine according to the absorption property of the concrete kind of photo bio and carrier 4 is common.; if what the photo bio cell of cultivating can be stable is adsorbed on carrier 4; can after the liquid immersing in pond body 1, not depart from and flow to (or only having micro-photo bio cell detachment carrier 4) in pond body 1 by carrier 4, in pond body 1, hold nutritive medium, can be regarded as and carry out solid culture.And if the photo bio cell of cultivating can flow to (or relatively a large amount of photo bio cell detachment carrier 4) in pond body 1 by carrier 4 disengagings after the liquid immersing in pond body 1, in pond body 1, hold photo bio culturing liquid, with in the time that each carrier 4 immerses, supplement the photo bio cell that flows into photo bio culturing liquid, the photo bio maintaining on carrier 4 is cultivated quantity, can be regarded as liquid culture.Certainly, can understand, in the nutritive medium of above-mentioned solid culture, not contain photo bio cell completely, but in solid culture the content of the photo bio cell in nutritive medium far fewer than liquid culture in the content of photo bio cell.In the present embodiment, what in pond body 1, hold is nutritive medium, adopts solid culture mode.
In the present embodiment, the first strut member 3 is made up of the rope made of hemp of 0.05 meter of diameter, 5 meters of overall lengths, and the 0.035 meter of Manila rope in two ends and diameter is connected.On supporting bar, be provided with slide rail, and be provided with the pulley that can slide up and down with respect to slide rail, the above-mentioned rope made of hemp is connected in pulley, slides on slide rail through motor drive pulley.Thus, motor drive the first strut member 3 moves down and just can make carrier 4 immerse in nutritive medium to supplement the nutritive medium on carrier or make carrier 4 immerse in photo bio culturing liquid with supply nutritive medium and introduce new photo bio cell simultaneously, and motor drive the first strut member 3 moves up and just can make liquid level that carrier 4 departs from nutritive medium or photo bio culturing liquid to be all exposed under sunlight.Wherein, in the time that the present embodiment is used for to solid culture, herein for to make carrier 4 depart from the liquid level of nutritive medium; If adopt liquid culture in remaining embodiment time, herein for to make carrier 4 depart from the liquid level of photo bio culturing liquid.As above,, by the circulation device of nutritive medium or photo bio culturing liquid is provided for carrier, can in illumination abundance, guarantee that photo bio cell grows quickly.Certainly, in embodiment alternatively, the first strut member 3 can be by bamboo and wood, synthetic plastics, nylon, fiber crops, soak and mould metal and make, and can preferably be made up of water-fast, impregnating material, have good rigidity or snappiness.Can understand, in the time that the first strut member 3 is flexible piece, on it, the carrier 4 of suspention may cause the slight bending of the first strut member 3, now can be regarded as each carrier 4 suspends in midair as the axis of vertical the first strut member 3 when bending, the axis while not suspending each carrier 4 in midair in the rotating mode of the axis perpendicular to the first strut member 3.Certainly, also can understand, because carrier 4 is to hang on the first strut member 3, be subject to the direction of gravity so the axis of its rotation is parallel to it.In other words, be not limited to this expression form, carrier 4 can rotate according to the direction of illumination of sunlight in the time of suspention, to avoid the mutual shading between carrier.
Further narration below, by the process of above-mentioned photo bio cultivation apparatus cultivation photo bio, take cultivating microalgae as example.
In breeding process, can directly in pond, supplement the larger carbon source of micro algae growth consumption and (contain CO
2gas mixture, pure CO
2gas, flue gas, carbonate, supercarbonate etc.), phosphorus source (phosphoric acid salt), nitrogenous source etc., and the sterilizing agent polluting for prevention and control disease and pest, then by stirring or the mode such as aeration, the nutritive substance, the sterilizing agent etc. that fill into are uniformly distributed in pond body 1.
Certainly, the structure of pond body is not limited to form shown in Fig. 1, dividing plate is set in can pond body 1 pond body 1 is divided into multiple sub-tanks, and nutrition/sterilizing agent can supplement respectively in sub-tank, also the nutritive medium in pond body 1 can be collected to rear unification and supplement.Or multiple ponds body is set, the corresponding one or more carrier settings of pond body.
In addition, in this embodiment for preventing that the mutual attaching of adjacent carrier 4 can be by increasing by 4 spacing of carrier or increasing by 4 times narrow end counterweights of carrier or increase interval supports 4 of adjacent carrier.And in this embodiment the movement velocity of the first strut member 3 and the residence time in daylight adjust and set according to actual weather condition.Also can be provided with light intensity feedback system, the direction and intensity of light intensity feedback system measures illumination is also controlled the motion of guiding piece 6 automatically according to detected result, automatically control thus the rotational angle of carrier 4.In addition, carrier 4 in the present embodiment also can move along the axis direction of the first strut member 3 simultaneously, the spacing of adjacent carrier 4 can be regulated according to the growing state of illumination and algae self thus, to improve spectrophotometric result and to meet under the prerequisite of illumination requirement, more photo bio cell can be cultivated.
In this embodiment, the material of pond body 1, supporting bar 7, the first strut member 3, carrier 4, guiding piece 5 and circulation device, structure, size, quantity and the first strut member 3 spacing and the period of motion etc. are all not limited to this.Can realize " dipping " nutritive medium; make microalgae cell carry out photosynthesis growth on implicit the carrier of nutritive substance; and can be according to the variation of light intensity and direction of illumination; regulate carrier angle (being similar to blind window), so that microalgae cell obtains any embodiment of more suitable growth conditions all in protection domain.
With reference to Fig. 5, in second embodiment of the present invention, wherein the place identical with first embodiment shown in Fig. 1 repeats no more.First strut member 3 of the present embodiment is made up of bamboo pole.Circulation device comprises that the cloth liquid part 8(being arranged on resting support 2 is as shown in Figure 6) and infusion set, the liquid outlet that cloth liquid part 8 comprises fluid inlet and arranges towards carrier 4, the fluid inlet of cloth liquid part 8 is communicated with infusion set.And the fluid inlet that infusion set can pump into the nutritive medium in pond body 1 or photo bio culturing liquid cloth liquid part 8 (in the present embodiment, in pond body 1, accommodate nutritive medium, carry out solid culture, so infusion set pumps into the nutritive medium in pond body 1 fluid inlet of cloth liquid part 8).In the present embodiment, cloth liquid part 8 is liquid distributor, and infusion set is water pump.Wherein, cloth liquid part 8 is made up of the plastics tubing of the about 1cm of internal diameter, is equipped with the liquid outlet that diameter is 0.5-1mm on it, and nutritive medium or photo bio culturing liquid drop in length evenly, slowly via the liquid outlet of cloth liquid part 8 to be had on the carrier 4 of microalgae cell.Can understand, quantity and the position of liquid outlet is set according to the spacing between the quantity of carrier 4 and two carriers 4, supplying with enough nutritive mediums by the photo bio cell on carrier 4.
With reference to Fig. 7, in the 3rd embodiment of the present invention, wherein the place identical with second embodiment shown in Fig. 5 repeats no more.In the present embodiment, carrier 4 is made up of 50 order nylon mesh, in pond body 1, be photo bio culturing liquid (nutritive medium and microalgae cell), photo bio culturing liquid evenly drops on carrier (carrier without in algae liquid as shown in embodiment 1 and 2 preculture 4 days) via water pump, cloth liquid part, make photo bio culturing liquid on carrier, form the thin liquid layer flowing, so that photo bio cell is fully shown in light, carry out photosynthesis.When in pond body 1, microalgae density reaches 2-5g/L, in collecting tank body 1, part microalgae cell to microalgae density in pond is 1-2g/L, starts a new round and cultivates.
With reference to Fig. 8 and Fig. 9, the 4th specific embodiment of the present invention, wherein exists together and repeats no more mutually with first embodiment.In the present embodiment, each carrier 4 is the rod-like element of suspending in midair slidably along the first strut member 3, and carrier 4 can move along the axis direction of the first strut member 3.With further reference to Fig. 8, in the present embodiment, the first strut member 3 is made up of thick 0.035 meter of rope made of hemp, carrier 4 is made up of white fiber rope (long 1.5 meters, thick 0.02 meter), on same the first strut member 3, the spacing of adjacent two carriers is 0.05 meter, establish altogether 8 the first strut members 3, the spacing of every adjacent two the first strut members 3 is 0.05 meter.Cultivating algae kind is limnetic chlorella, within every 4 minutes, the first strut member 3 slowly moves to pond body 1 under the effect of electric motor, carrier on it (carrier without in algae liquid as described in Example 1 preculture 4 days) is immersed in photo bio culturing liquid (nutritive medium and microalgae cell), after carrier 4 is completely submerged, motor drive the first strut member 3 with its on each carrier 4 rise from liquid level and be placed under daylight, now photo bio culturing liquid forms the thin liquid layer flowing on carrier 4, so that microalgae cell is fully shown in light, carry out photosynthesis, and so forth, and staggered the carrying out that move up and down of each the first strut member.In the time that light intensity is crossed by force or is grown with inhibition or restriction microalgae cell a little less than crossing, can be made by the rope made of hemp by pulling the first strut member 3(, be flexible piece) relative position of 4, carrier on different the first strut members 3 is changed, thus make microalgae cell obtain suitable light intensity.When in the body of pond, microalgae density reaches 2-5g/L, in collecting tank, part microalgae cell to microalgae density in pond is 1-2g/L, starts a new round and cultivates.
The present embodiment can be according to the variation of light intensity, direction of illumination and microalgae cell growth phase demand, regulate the spacing with the adjacent carrier 4 on root the first strut member 3, so that microalgae cell obtains more suitable growth conditions, thereby improve light utilization efficiency, to improve micro-algae output
With reference to Figure 10, in the 5th embodiment, wherein, with second identical repeating no more of embodiment.In the present embodiment, the first strut member 3 is directly fixing with supporting bar 7.The first strut member 3 is made up of the bamboo pole of thick approximately 0.03 meter, carrier 4 is made up of the thick cotton cord of white 1.5 meters of the length of the cotton cord (, thick 0.02 meter), on same the first strut member 3, the spacing of adjacent two carriers 4 is 0.05 meter, establish altogether 8 the first strut members 3, the spacing of every adjacent two the first strut members 3 is 0.05 meter.Cloth liquid part 8 is made up of the plastics tubing of the about 1cm of internal diameter, is provided with the liquid outlet that diameter is 0.5-1mm on it, and nutritive medium flows in cloth liquid part 8, and drops on carrier 4 via liquid outlet.Cultivating algae kind is limnetic chlorella.In the time that light intensity is crossed by force or grown with inhibition or restriction microalgae cell a little less than crossing, can the relative position between carrier on different the first strut members be changed by pulling the first strut member, thereby make microalgae cell obtain suitable light intensity.In this embodiment, the first strut member is adjusted, is set according to actual weather condition the regulating effect of carrier; The first strut member can, by being connected with light intensity feedback system, be realized automatically and regulating.
Certainly, photo bio cultivation apparatus of the present invention is not limited to said structure.For example, preferably, carrier 4 is concave/convex-shaped structure or the structure that is provided with through hole, and the surface of carrier 4 has depression and projection, or carrier 4 is provided with through hole.Concave/convex-shaped structure and the structure that is provided with through hole all can improve the liquid ability of holding of carrier, in the time hanging carrier 4 can maintain wherein more liquid can be because deadweight departs from carrier 4.Thus, can reduce the frequency of utilization of circulation device, to reduce costs.In addition, when when carrier being immersed to the mode of mentioning again after nutritive medium or photo bio culturing liquid as carrier Additional nutrient solution or photo bio culturing liquid, concave/convex-shaped structure and the structure that is provided with through hole can be stayed more nutritive mediums or photo bio culturing liquid in carrier 4 mentioning in process.And each carrier 4 can be suspended on the first strut member 3 perpendicular to the rotating mode of the axis of the first strut member 3, or can be suspended to slidably on the first strut member 3 along the first strut member 3, or be suspended on the first strut member 3 in the mode of above-mentioned rotation in, be suspended to slidably on the first strut member 3 along the first strut member 3, realize simultaneously and rotating and the motion of sliding.Certainly, select which kind of mode of motion to depend on kind, the situation of illumination etc. of the photo bio of cultivation, be not limited to certain mode of motion, effectively utilize luminous energy realizing, improve output.
In addition, in the embodiment alternatively of photo bio cultivation apparatus of the present invention, also can comprise the travel mechanism that drives resting support to move, better to make carrier follow the larger sensitive surface of mobile formation of the sun.For example, in the time that pond body 1 is circular pond body, travel mechanism can drive resting support to rotate around the axis of circular pond body.
Certainly, whether the setting of pond body 1 can be basis according to whether needing to reclaim the nutritive medium that carrier 4 drips, and apply take the above-mentioned method that carrier 4 is dipped in pond body 1 to nutritive medium as basis.And this device can be built in original raceway pond, natural hole, pond, lakeside, make its fixing input low.And this device does not need to carry a large amount of water bodys (nutritive medium/photosynthetic microorganism nutrient solution), thereby water consumption, operation energy consumption and the equipment, the facility that have reduced breeding process drop into.In addition, in this device, carrier 4 can slowly, vertically pass in and out nutritive medium, resistance is little, and can be by gravitional force and other natural energy resources, therefore total energy consumption is low.In addition, being oppositely arranged of pond body and carrier is also not limited to the structure that above-described embodiment is described.Particularly, can a pond body be set corresponding each carrier.Be interconnected or be isolated from each other corresponding to the Chi Tike of each carrier.
Alternatively, realize the mode that the first strut member moves along supporting bar and be not limited to above-described embodiment, for example, pulley is set on supporting bar, the stationary rope respectively at the two ends of the first strut member, rope through pulley with utilize lever principle that the first strut member is moved down or on carry.And drive such as electric motor of the optional use of drive element rope motion or that drive the first strut member to move along supporting bar, and propulsion source is preferably the eco-friendly power source such as sun power, tidal energy.
The foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, for a person skilled in the art, the present invention can have various modifications and variations.Within the spirit and principles in the present invention all, any modification of doing, be equal to replacement, improvement etc., within all should being included in protection scope of the present invention.
Claims (11)
1. a photo bio cultivation apparatus, is characterized in that, comprising:
At least one resting support (2); And
The carrier (4) of at least two isolated, adsorbable photo bio cells, nutritive medium and photo bio culturing liquids, each described carrier (4) hangs on described resting support (2).
2. photo bio cultivation apparatus according to claim 1, is characterized in that,
Described resting support (2) has the first strut member (3), each carrier (4) or be suspended to described the first strut member and/or suspend in midair slidably along described the first strut member in the rotating mode of axis perpendicular to described the first strut member (3).
3. photo bio cultivation apparatus according to claim 2, is characterized in that,
Described carrier (4) is sheet shaped piece, by guiding piece (6), all described sheet shaped piece on same described the first strut member (3) is together in series, and described guiding piece (6) is spaced apart from each other with described the first strut member (3).
4. photo bio cultivation apparatus according to claim 2, is characterized in that,
Described carrier (4) is the rod-like element of suspending in midair slidably along described the first strut member (3).
5. according to the photo bio cultivation apparatus described in any one in claim 1 to 4, it is characterized in that,
Described carrier (4) is made up of one or more materials in glass fibre, nylon, cotton, fiber crops, carbon fiber, synthon, sponge, plastic foam, metal, synthetic plastics.
6. according to the photo bio cultivation apparatus described in any one in claim 1 to 4, it is characterized in that,
Described carrier (4) comprises the covering part of base portion and the described base portion of covering, and described covering part is made up of the sorbing material of adsorbable photo bio cell, nutritive medium and photo bio culturing liquid.
7. according to the photo bio cultivation apparatus described in any one in claim 1 to 2, it is characterized in that,
Described carrier (4) is concave/convex-shaped structure or the structure that is provided with through hole.
8. photo bio cultivation apparatus according to claim 1, is characterized in that, also comprises:
For described carrier (4) provides the circulation device of nutritive medium or photo bio culturing liquid.
9. photo bio cultivation apparatus according to claim 8, is characterized in that, described circulation device comprises:
Be arranged at the cloth liquid part (8) on described resting support (2), the liquid outlet that described cloth liquid part (8) comprises fluid inlet and arranges towards described carrier (4); And
The infusion set being communicated with the fluid inlet fluid of described cloth liquid part (8).
10. photo bio cultivation apparatus according to claim 8, is characterized in that,
Described resting support (2) comprising: two compartment of terrains and the supporting bar (7) being arranged in parallel; Two ends are connected in respectively first strut member (3) of two described supporting bars (7) movably, and wherein said carrier (4) hangs on described the first strut member (3);
Described circulation device comprises: drive described the first strut member (3) to come and go mobile drive element along described supporting bar (7).
11. photo bio cultivation apparatus according to claim 1, is characterized in that,
Be provided with at interval multiple resting supports (2), and the orientation of described multiple resting support (2) is perpendicular to the orientation of the carrier on same resting support.
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| CN201410077552.2A CN103820318B (en) | 2014-03-04 | 2014-03-04 | Photo bio cultivation apparatus |
| PCT/CN2015/073651 WO2015131830A1 (en) | 2014-03-04 | 2015-03-04 | Photosynthetic organism cultivation device |
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| WO2015131830A1 (en) | 2015-09-11 |
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