JP5324532B2 - Circulating photobioreactor - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a circulating optical bioreactor. <P>SOLUTION: A first culture part includes a culture tank to which a culture solution is fed, and a first light source connected to the culture tank to emit light into the culture tank. A second culture part includes culture piping which is disposed as pipes outside the culture tank and to which the culture solution is fed from the culture tank, and a second light source connected to the culture piping to emit light into the culture piping. A pump part is connected between the first culture part and the second culture part to circulate the culture solution between the first culture part and the second culture part. <P>COPYRIGHT: (C)2012,JPO&amp;INPIT

Description

  The present invention relates to a culture apparatus, and more particularly to a circulating photobioreactor.

  Currently, crops that are cultivated all over the world have been used as the main food source for human beings, but the yield per farmland is not large and the utilization rate of solar energy is very low. Compared to this, fine photosynthetic algae that can grow in water with solar energy, carbon dioxide, and small amounts of inorganic salts can grow in areas where crops cannot grow. Thus, it is possible not only to obtain 20 times or more protein per unit cultivation area, but also to produce various kinds of useful substances and natural rare substances that cannot be produced from microorganisms or animal and plant cells. In particular, algae with large cell size can be easily precipitated and extracted and separated by various methods, and solar energy irradiated on the earth can be obtained by using solar energy as a main energy source. It can be used efficiently, has a photosynthesis process that uses carbon dioxide as a carbon source and releases oxygen as a by-product, and has a function of reducing air pollution.

  As a result, mainly the genus Chlorella, the genus Dunaliella, and the genus Spirulina, among these, the photosynthesis microalgae of the genus Spirulina (hereinafter referred to as “Spirulina” for convenience) have other photosynthesis properties. Compared to microalgae, the size of the cells is large, and algae that can grow easily even in an alkali-contaminated environment, has been applied to various products such as foods, pharmaceuticals, and industrial products. It is being actively promoted. On the other hand, South Korea has developed a technique related to indoor culture rather than outdoor culture of Spirulina because of the climatic characteristics that the seasons are obvious and the temperature changes and the amount of sunshine vary greatly.

  For example, Patent Document 1 discloses a culture tank equipped with an automatically adjusting heater and an aeration device, a support frame equipped with equipment for supporting the culture tank, and a culture water supply attached to a side surface of the support frame. A chain culture apparatus including a pipe for use, a rubber pipe for circulating culture water, and a water pump for circulating culture water is disclosed. In Patent Document 2, a culture vessel is formed into a double cylinder formed of an inner cylinder and an outer cylinder arranged horizontally, and at the same time, at least the outer cylinder is made of a transparent material that transmits visible light, and a gas inlet is provided. An apparatus for culturing microalgae opened at the lower part in a culture vessel is disclosed. Patent Document 3 discloses an outdoor culture tank for water channel type microalgae in which an inoculum culture tank in which an inoculum is cultured is integrally provided inside a large-scale culture tank for microalgae culture. However, when spirulina is cultivated using these culture devices, the spirulina grown on the surface of the culture device is excessively fixed, and the culture efficiency decreases over time. There was a disadvantage that it could not be obtained.

  In order to overcome these problems, a technique has been developed in which a culture tank is sealed and a filter is provided in the air that flows in to prevent contamination of germs. For example, Patent Literature 4 discloses an outdoor mass culture apparatus for microalgae including an air filtration and ultraviolet sterilizer, an air pump, a filtration device, a guide tube, a filter, a needle valve, and an incubator. High density for microalgae, including a culture tank with a lid on the top and a pH sensor and disperser inside, a culture tank frame with a fluorescent lamp, a pH controller, an air pump and a carbon dioxide culture tank A culture apparatus is disclosed.

Korean Patent Registration No. 235182 Korean Patent Registration No. 609736 Korea Patent Registration No. 679989 Korean Patent Publication No. 2002-0057882 Korean Patent Registration No. 442092

  However, although it was possible to prevent contamination by various bacteria using the above-described conventional apparatus, the photosynthesis microalgae grown on the surface of the culture apparatus are excessively fixed, and the culture efficiency decreases over time. The problem is the situation that has not been solved. If such problems can be solved, the cultivation efficiency of photosynthesis microalgae is expected to increase significantly, and photosynthesis microalgae are expected to be produced more economically. However, no results have been reported yet. Not. The present invention provides a photobioreactor capable of increasing the culture efficiency of photosynthesis microalgae by reducing the fixation of photosynthesis microalgae.

  As a result of earnest research efforts to solve the problem that the photosynthesis microalgae grown on the surface of the culture apparatus are excessively fixed, the present inventors have determined that when the photosynthesis microalgae are cultured in a circulating manner, The area where the algae culture solution was exposed to light was maximized, and it was confirmed that the photosynthesis microalgae grown on the surface of the culture apparatus were not excessively fixed by circulation of the culture solution, thereby completing the present invention.

  A photobioreactor according to an aspect of the present invention is provided. The first culture unit is provided to include a culture tank to which a culture solution is supplied and a first light source coupled to the culture tank so as to irradiate the inside of the culture tank. The second culture unit is arranged in a tubular shape outside the culture tank, and is coupled to the culture pipe so as to irradiate light into the culture pipe supplied with the culture solution from the culture tank. Provided to include a second light source. The pump unit is connected between the first culture unit and the second culture unit so as to circulate a culture solution between the first culture unit and the second culture unit.

  A photobioreactor according to another aspect of the present invention is provided. The first culture unit includes a culture tank to which a culture solution is supplied, a first culture solution inlet connected to the culture tank, a first culture solution discharge port, and a first light source. The second culture unit is arranged in a tubular shape outside the culture tank, a culture pipe to which a culture solution is supplied from the culture tank, a second culture solution inlet connected to the culture pipe, and a second culture solution drain An outlet and a second light source are included. The first culture solution outlet is connected to the second culture solution inlet so that the culture solution is circulated between the first culture unit and the second culture unit. Is connected to the second culture medium outlet.

  If the photobioreactor according to the embodiment of the present invention is used, it is possible to prevent the photosynthetic microalgae from sticking to the surface of the culture apparatus and increase the culture efficiency of the photosynthesis microalgae. Thereby, the culture | cultivation efficiency of photosynthesis microalgae improves and it can ensure economical efficiency.

It is a schematic diagram showing one embodiment of the circulation type photobioreactor of the present invention. It is sectional drawing which shows one Embodiment of the culture part contained in the circulation type photobiological reactor of this invention. It is a top view which shows one Embodiment of the piping part contained in the circulation type photobiological reactor of this invention. It is a schematic diagram which shows one Embodiment of the oxygen exhaust device with which the culture piping of the circulation type photobiological reactor of this invention was equipped. The top view which shows other embodiment of the culture | cultivation part provided with the two culture solution discharge ports which supply a culture medium to two pump parts, and the two culture solution inflow ports into which a culture solution flows in from two culture pipings. It is.

  The present inventor conducted various studies to grasp the cause of excessive fixation of the photosynthesis microalgae on the surface of the culture apparatus when culturing the photosynthesis microalgae containing spirulina. As a result, two causes of excessive fixation of photosynthesis microalgae have been grasped. One is that light conditions and dark conditions are regularly provided when culturing photosynthesis microalgae. As a result, not only the growth of the photosynthesis microalgae but also the growth of the population is accompanied, and the other one is that the culture solution hardly flows during the culture of the photosynthesis microalgae, or The fixation of the photosynthesis microalgae begins at a site where the flow of the culture solution is limited and the flow rate of the culture solution is small, and the fixed region is expanded.

  In order to eliminate such a cause, the present inventor only reduces the growth rate of the population of the photosynthesis microalgae by providing only bright conditions during the cultivation of the photosynthesis microalgae, Although an attempt was made to devise a culture apparatus that can be made to flow, when using a photobiological reactor in the form of a culture tank equipped with a stirrer, the culture solution can flow sufficiently even if the stirring speed of the stirrer is increased. I confirmed that I couldn't.

  As a result, we tried to make the culture solution containing photosynthesis microalgae flow in another method that is not simply a method using a stirrer, but when using a method in which the culture solution itself is circulated, a simpler method is used. It was also confirmed that the culture solution can be sufficiently fluidized. In order to realize this, the present inventors have devised a photobioreactor including a culture pipe and a pump in which a light source is attached to the photobioreactor. Unlike the conventional photobioreactor, the photobioreactor can flow the culture solution in a manner that circulates the culture solution at a constant speed using a culture pipe and a pump, and is coupled to the culture pipe. By using a light source, it was possible to easily provide bright conditions to the culture solution and to supply a sufficient amount of sunlight during the cultivation of the photosynthesis microalgae.

  Hereinafter, a configuration of a photobiological reactor according to an embodiment of the present invention will be described in detail with reference to the accompanying drawings.

  FIG. 1 is a schematic diagram showing a photobioreactor according to an embodiment of the present invention. As shown in FIG. 1, the photobioreactor may include a first culture unit 100, a pump unit 200, and a second culture unit 300. The first culture unit 100, the pump unit 200, and the second culture unit 300 are in communication with each other, and the culture solution is in the order of the first culture unit 100 → the pump unit 200 → the second culture unit 300 → the first culture unit 100. Cultivate photosynthetic microalgae while circulating in

  The second culture unit 300 may be spaced apart from the first culture unit 100. As shown in FIG. 1, the first culture unit 100 may be provided in a cylindrical shape, but this embodiment is not limited thereto. For example, the shape of the first culture unit 100 can be variously modified into a polygonal cylinder. The second culture unit 300 is provided in a tubular shape, and may include, for example, piping formed into various shapes.

  The pump unit 200 can be configured such that the culture solution circulates between the first culture unit 100 and the second culture unit 300. For example, the pump unit 200 exists between the first culture unit 100 and the second culture unit 300, and is connected to the first culture solution outlet 132 of the first culture unit 100. A third culture medium outlet 230 communicating with the pump 220 and the second culture medium inlet 310 of the second culture unit 300 may be provided. As a result, the culture solution flowing from the first culture unit 100 is transmitted to the second culture unit 300. As a result, the culture solution is changed from the first culture unit 100 → the pump unit 200 → the second culture unit 300 → first. Circulate in the order of the culture unit 100. At this time, the pump unit 200 may additionally include a flow rate controller. However, the flow rate controller may be built in the pump 220 of the pump unit 200, and the pump may be electrically connected to the pump. Can be attached to the culture tank 101 or the culture pipe 330. Flow rate regulation is a very important factor for efficient cultivation of photosynthesis microalgae. If the flow rate is too slow, algae such as spirulina, which is a multicellular spiral microalgae, can be attached to the inner wall of the culture pipe 330 and the hydrodynamics conditions are not met. Degassing, gas exchange and light irradiation are not smooth, and there is a disadvantage that productivity of photosynthesis microalgae decreases.On the other hand, if the flow rate is too high, the loss of useful substances inside the microalgae This is because it occurs. Accordingly, the flow rate is preferably adjusted to 1 to 50 cm / s, more preferably 10 to 40 cm / s, and most preferably 20 to 30 cm / s, but is not limited thereto. Absent.

  FIG. 2 is a cross-sectional view showing an embodiment of the first culture unit 100 included in the photobiological reactor of the present invention. As shown in FIG. 2, the first culture unit 100 includes a cylindrical culture tank 101, an inoculation inlet 110, a gas inlet 111, a sensor mounting port 120, a first culture fluid inlet 131, and a first culture fluid. The discharge port 132 and the final discharge port 133, the pressure control valve 112 and the culture solution supplier 134, the first light source 140 and the stirrer 150, and the temperature controller 160 may be combined.

  For example, the inoculation inlet 110 is coupled to the upper part of the culture tank 101, the gas inlet 111 and the sensor mounting port 120 are coupled to the lower part of the culture tank 101, and the pressure control valve 112 and the culture medium inlet 134 are the culture tank. 101 can be coupled to the top. However, such an arrangement is provided by way of example, and can be variously modified by changing the shape of the culture tank 101 or the like.

The inoculation inlet 110 is provided to inject an inoculum into the culture tank 101, and the gas inlet 111 injects various gases, for example, a mixed gas of nitrogen and carbon dioxide, into the culture tank 101. May be offered to do. Thereby, by maintaining the internal pressure of the culture tank 101 at a higher positive pressure than the outside, it is possible to prevent inflow of germs derived from the outside during the cultivation of the photosynthesis microalgae. At this time, the range of the positive pressure is not particularly limited, but is preferably 0.1 to 1.0 kg / cm ² f used in normal microalgae culture.

  Various sensor devices such as a pH sensor, a carbon dioxide concentration sensor, a dissolved oxygen concentration sensor, and a temperature sensor can be attached to the sensor attachment port 120 in order to confirm the culture state of the culture being cultured.

  Meanwhile, the culture medium that has moved from the second culture unit 300 through the first culture solution inlet 131 flows into the first culture unit 100, and passes from the first culture unit 100 to the pump unit 200 through the first culture solution outlet 132. After the culture solution is flowed out and the culture is completed, the culture is discharged to the outside of the first culture unit 100 through the final discharge port 133.

  In addition, the pressure control valve 112 is a one-way valve that is opened and closed by the pressure of oxygen generated during the cultivation of the photosynthesis microalgae, and the internal pressure of the culture unit is positive compared to the external pressure. When the internal pressure is maintained, the valve is opened and the gas is discharged to the outside. However, when the internal pressure is reduced, the valve is closed and the gas discharge is stopped.

  The medium injection port 134 is a rod-shaped tube having a spherical end, and may be provided in the form of a spray ball having a large number of pores on the surface of the spherical end. Through this, a fresh medium is distributed and supplied to the first culture unit 100 with a fine water flow, whereby the medium injection port 134 can also serve to remove bubbles generated inside the culture unit, and the culture is completely completed. And after the culture is discharged, it is also used for supplying a cleaning agent for cleaning the inside of the first culture unit 100, the pump unit 200, and the second culture unit 300.

  The first light source 140 is a device that emits light capable of performing photosynthesis when cultivating photosynthesis microalgae, and emits light having three or five wavelengths similar to natural light. It is desirable to automatically vary the illuminance and light / dark cycle of the light source depending on the culture conditions. For example, the first light source 140 is disposed inside the culture tank 101. As another example, when the culture tank 101 is made of a material that transmits light, or a daylighting window capable of transmitting light is provided in a part of the culture tank 101, the first light source 140 is used for the culture. It may be disposed on the outer surface of the tank 101.

  The stirrer 150 is provided at the inner bottom of the culture tank 101 and plays a role of mixing the culture solution during the initial culture of the photosynthesis microalgae. From the culture solution remaining in the culture section and the culture solution inlet port even during the main culture. It performs the role of mixing the flowed culture. The temperature controller 160 may be attached to the outside of the first culture unit 100 to adjust its temperature. The temperature controller 160 is not particularly limited, but is a water jacket that can adjust a desired culture temperature by circulating water at an appropriate temperature through the jacket. . At the same time, the first culture unit 100 may further include a viewing window through which the inside can be confirmed.

  FIG. 3 is a plan view showing an embodiment of the second culture unit 300 included in the tubular photobioreactor of the present invention. As shown in FIG. 3, the second culture unit 300 includes a second culture solution inlet 310, a culture pipe 330, and a third culture solution discharge port 230 connected to the pump unit (200 in FIG. 1). A second culture medium outlet 340 communicated with the first culture medium inlet 131 of the culture unit is provided.

  The second light source 320 may be attached to a part or the whole of the second culture unit 300. For example, the second light source 320 may be coupled to the outer surface of the culture pipe 330 so as to extend along the longitudinal direction of the culture pipe 330. The culture pipe 330 of the second culture unit 300 is supplied with light from the second light source 320 while circulating a culture solution containing the photosynthesis microalgae therein, and the photosynthesis microalgae performs photosynthesis. Can play a role to provide an environment that can Accordingly, the culture pipe 330 is made of a material that can transmit light emitted from the second light source 320. For example, the entire culture pipe 330 may be made of a material that can transmit light. It is also possible to use a material that allows light to pass through only the portion provided with the light source. Optionally, the second light source 320 is disposed in the culture pipe. In this case, the second light source 320 is preferably provided on the inner wall of the culture pipe, and is preferably configured by an LED element. In this case, the culture pipe can be made of an opaque material. In addition, for convenience during culture, a sensor device such as a pH sensor, a carbon dioxide concentration sensor, a dissolved oxygen concentration sensor, or a temperature sensor may be further provided at one end of the culture pipe 330. At the same time, the culture pipe 330 is preferably formed in an elongated tube shape so as to maximize the area where the culture solution is exposed to light. In order to efficiently arrange the pipes 330, it is desirable to configure the pipes 330 in a multiple and folded form. In this case, the second light source 320 may be provided in a multiple manner between the folded structures of the culture pipe 330. The inner diameter of the culture pipe is preferably 3 to 30 cm, more preferably 5 to 25 cm, and most preferably 10 to 20 cm. The internal diameter of the culture pipe is an important factor for efficient cultivation of the photosynthesis microalgae. If the internal diameter is too large, the light irradiation is insufficient and the productivity is lowered. If the inner diameter is too small, it is difficult to adjust the flow rate and scale-up.

  According to an embodiment of the present invention, the culture pipe 330 of the second culture unit 300 of the present invention is configured in a form that is folded in parallel and includes a straight part and a bent part on a single frame. In this way, the folded form can be configured to be superimposed in multiple stages. In addition, the frame may be a frame for fixing the second light source 320 and the culture pipe 330, and may further include a power supply device for supplying power to the second light source 320. At the same time, the second light source 320 provided in the culture pipe 330 plays a role of diverging light capable of photosynthesis during the cultivation of the photosynthesis microalgae, and the wavelength of the light emitted from the second light source 320. Although it is not particularly limited to this, three or five wavelengths similar to natural light are desirable, and it is desirable that the illuminance and light-dark cycle of the light source are automatically varied depending on the culture conditions.

  On the other hand, since the culture pipe 330 is configured in multiple folded forms including a straight part and a bent part, oxygen produced during the cultivation of photobiology may be accumulated in the bent part without being discharged. . In order to forcibly discharge the accumulated oxygen, if the oxygen is accumulated to a certain level or more by further providing an oxygen ejector at the bent portion, the oxygen ejector (see FIG. 4) automatically operates. It is desirable to discharge the accumulated oxygen to the outside.

  FIG. 4 is a schematic diagram showing an embodiment of an oxygen discharger provided in the culture pipe 330 of the photobioreactor of the present invention. As shown in FIG. 4, the oxygen discharger includes a ground sensor 351, a flow controller (MFC) 352 electrically connected to the ground sensor, and a valve 353 connected to the flow controller. However, it is energized by immersing it in a culture solution at one end of the ground sensor 351.

  If oxygen is accumulated at the bent portion of the culture pipe 330 to form a gas layer, the ground sensor 351 is pushed out of the culture solution, and if the ground sensor is pushed out of the culture solution, current is maintained. In the case where the power is cut off, the valve 353 connected to the flow rate regulator 352 is operated to discharge a certain amount of oxygen. When a certain amount of oxygen is exhausted and the ground sensor 351 is immersed again in the culture solution, it is energized and the operation of the valve 353 connected to the flow rate regulator 352 is stopped.

  Meanwhile, according to another embodiment of the present invention, the tubular photobioreactor of the present invention may include one culture unit, two or more pump units, and a piping unit.

  FIG. 5 shows two first culture liquid outlets 132 for supplying cultures to two pump units, two first culture liquid inlets 131 through which cultures flow from two culture pipes 330, and one culture liquid. It is a top view which shows other embodiment of the culture part with which the discharge port 133 was provided. As shown in FIG. 5, if a tubular photobioreactor including one culture section and two or more pump sections and piping sections is used, one culture section is connected to two or more piping sections. Since the culture is supplied, the culture can be performed more efficiently.

  Hereinafter, operations and effects of the tubular photobioreactor of the present invention will be described in detail with reference to the accompanying drawings.

  First, after closing the first culture fluid inlet 131, the first culture fluid outlet 132, and the culture fluid outlet 133 of the first culture unit 100, the culture tank 101 is optically coupled through the inoculation inlet 1110 or the medium inlet 134. The inoculum of inoculated microalgae seeds is injected, light is supplied to the first light source 140, and the stirrer 150 is driven while maintaining an appropriate temperature through the temperature controller 160, so that the photosynthesis microalgae is cultured for the first time. To implement. The initial culture is terminated at an appropriate time using various sensors mounted on the sensor mounting port 120 of the first culture unit 100 while checking the culture state.

  Thereafter, while injecting a fresh medium into the culture tank 101 after the initial culture through the medium injection port 134, the stirrer 150 is continuously driven to mix the culture liquid after the initial culture with the culture liquid. When the culture medium having the same volume as the culture is injected, the first culture liquid inlet 131 and the first culture liquid outlet 132 are opened, and the mixed culture liquid is supplied to the third portion of the pump unit 200. It is transmitted to the culture fluid inlet 210. When the mixed culture medium is transmitted to the third culture solution inlet 210 of the pump unit 200, the pump 220 of the pump unit 200 is driven to supply the culture solution to the second culture through the third culture solution outlet 230. The culture medium transmitted to the second culture fluid inlet 310 of the unit 300 and transmitted to the second culture fluid inlet 310 of the second culture unit 300 is supplied to the culture pipe 330 and the second culture fluid outlet by the pump 220. 340 and the first culture fluid inlet 131 of the first culture unit 100 are sequentially transmitted to the culture tank 101, so that the culture fluid starts to circulate. As described above, when the circulation of the culture solution starts, light is supplied from the second light source 320 attached to the culture pipe 330 to the culture pipe 330, and the photosynthesis microalgae contained in the culture solution starts photosynthesis. At this time, it is desirable that the pump 220 of the pump unit 200 is additionally provided with a flow rate adjuster, and the flow rate of the culture medium circulating through the culture pipe 330 is adjusted by the flow rate adjuster. The adjustment of the flow rate of the circulated culture solution is very important. This is because when the speed is too slow, photosynthesis microalgae, especially multicellular spiral microalgae such as Spirulina, adhere well to the inner wall of the culture pipe 330 and are caused by improper flow velocity motility. This is because degassing, gas exchange, etc. are poor, the photosynthesis efficiency is very low, and when the speed is too high, the loss of useful substances inside the microalgae is caused. Accordingly, the flow rate is desirably adjusted to 1 to 50 cm / s, more desirably 10 to 40 cm / s, and most desirably 20 to 30 cm / s.

While culturing such circulating photosynthesis microalgae, a mixed gas of carbon dioxide and nitrogen is continuously injected through the gas injection port 111 of the first culture unit 100 to culture the culture tank of the first culture unit 100 101 is applied with a positive pressure of about 0.1 to 1.0 kg / cm ² f to prevent contamination of germs derived from the outside and adjust the partial pressure of carbon dioxide contained in the mixed gas. To adjust the pH of the culture solution.

  At the same time, oxygen generated by photosynthesis during the cultivation of the photosynthesis microalgae is separated from the medium in the culture tank 101 of the first culture unit 100, moves to the upper part of the culture tank, and is discharged to the outside through the pressure control valve 112. . At this time, when the culture pipe 330 of the second culture unit 300 of the present invention is configured to be folded in parallel and includes a straight part and a bent part on one frame, the bent part of the culture pipe 330 is formed. Oxygen is accumulated in the first culture unit 100 and may not be transmitted to the culture tank 101 of the first culture unit 100. Therefore, it is possible to remove the oxygen accumulated in the culture pipe 330 by attaching an oxygen discharger to the bent portion. desirable.

  When the above-described cultivation of the circulating photosynthesis microalgae is completed, the final outlet 133 of the first culture unit 100 is opened to obtain the finally cultured photosynthesis microalgae. Thus, after culture | cultivation of circulation type photosynthesis microalgae was complete | finished, as a result of calculating the ratio of the surface area of the culture piping 330 to which photosynthesis microalgae adhered with respect to the whole surface area of the culture piping 330, it was less than 5%. When using the tubular photobioreactor according to the present invention, the growth rate of the population of photosynthesis microalgae is maintained by maintaining only the light conditions with the light source provided along the culture pipe. And the culture fluid circulates through the culture section, circulation pump and piping section to maximize the flow of the culture liquid, so that the photosynthesis microalgae adheres to the surface of the culture apparatus and the culture yield decreases. I was able to solve the problem.

  The photosynthesis microalgae that can be cultured by the photobioreactor of the present invention is not particularly limited, but is preferably a microalga of the genus Chlorella, Donariella, or Spirulina, and is a microalga of the genus Spirulina. More desirable.

  The present invention is applicable to the field related to the circulation type photobioreactor.

Claims (16)

A first culture unit including a cylindrical culture tank to which a culture solution is supplied, and a first light source coupled to the culture tank so as to irradiate the inside of the culture tank;
Arranged in a tube folded multiple times outside the culture tank, the culture solution is supplied from the culture tank , the culture pipe having a diameter of 3 to 30 cm, and so that the inside of the culture pipe is irradiated with light, wherein comprises a second light source disposed so as to be stretched along the longitudinal direction of the culture pipe, an oxygen ejector provided in the bent portion of the culture pipe, a second culture part,
A pump unit connected between the first culture unit and the second culture unit so as to circulate a culture solution between the first culture unit and the second culture unit;
A photobiological reactor.   The photobiological reactor according to claim 1, wherein the first light source is arranged inside the culture tank. The first culture unit further includes at least one first culture fluid inlet and at least one first culture fluid outlet coupled to the culture tank and connected to the pump unit,
The second culture unit includes at least one second culture medium inlet and at least one first culture medium inlet connected to the at least one first culture medium outlet and the at least one first culture medium inlet through the pump unit. The photobioreactor according to claim 1 or 2 , further comprising a second culture medium discharge port. The first culture unit further includes a culture solution supplier for supplying a culture solution to the inside of the culture tank and a culture solution discharge port for releasing the culture solution from the culture tank. 4. The photobiological reactor according to any one of 3 . The culture broth is a rod-shaped tube, and the end of the broth is in a spherical shape, and the surface of the spherical end is in the shape of a spray ball having many pores. Item 5. The biobiological reactor according to Item 4 . The photobioreactor according to any one of claims 1 to 5 , wherein the first culture unit further includes a stirrer for mixing the culture solution in the culture tank. The photobioreactor according to any one of claims 1 to 6 , wherein the first culture unit further includes a pressure control valve for adjusting the pressure in the culture tank. The photobiological reactor according to claim 7 , wherein the pressure control valve is a one-way valve that is opened and closed by a pressure of oxygen generated during photobiological culture. The photobioreactor according to any one of claims 1 to 8 , wherein the first culture unit further includes a temperature controller for adjusting a temperature in the culture tank. The photobioreactor according to any one of claims 1 to 9 , wherein the first culture unit further includes a plurality of sensor mounting ports coupled to the culture tank. The first culture unit, said to permit the injection of gas into the culture tank, further comprising a combined gas inlet to the culture tank, according to any one of claims 1 to claim 10 Photobioreactor. The oxygen discharger includes a grounding sensor coupled to a bent portion of the culture pipe, a flow controller electrically connected to the grounding sensor, and a valve coupled to the flow controller. 2. The photobiological reactor according to 1. The photobioreactor according to any one of claims 1 to 12 , wherein the pump unit includes a flow rate regulator. A first culture unit including a cylindrical culture tank to which a culture solution is supplied; a first culture solution inlet coupled to the culture tank; a first culture solution discharge port; and a first light source;
A culture pipe with a diameter of 3 to 30 cm, which is arranged in a multiple folded tube outside the culture tank and is supplied with the culture liquid from the culture tank, and a second culture liquid inlet connected to the culture pipe And a second light source disposed so as to extend along the longitudinal direction of the culture pipe , an oxygen discharger provided at a bent portion of the culture pipe, a second culture medium discharge port, and a second light source 2 culture parts,
The first culture solution outlet is connected to the second culture solution inlet so that the culture solution is circulated between the first culture unit and the second culture unit. Is a photobioreactor connected to the second culture medium outlet. 15. The apparatus according to claim 14 , further comprising a pump unit coupled between the first culture unit and the second culture unit so as to circulate a culture solution between the first culture unit and the second culture unit. Photobioreactor. The pump part is
A third culture fluid inlet between the first culture fluid outlet and the second culture fluid inlet;
A third culture fluid outlet between the first culture fluid inlet and the second culture fluid outlet;
A flow rate regulator;
The photobioreactor of claim 15 , comprising:

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