CN105039138A - Microalgae culture system with solar cell panels and culture method thereof - Google Patents

Microalgae culture system with solar cell panels and culture method thereof Download PDF

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Publication number
CN105039138A
CN105039138A CN201510513083.9A CN201510513083A CN105039138A CN 105039138 A CN105039138 A CN 105039138A CN 201510513083 A CN201510513083 A CN 201510513083A CN 105039138 A CN105039138 A CN 105039138A
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CN
China
Prior art keywords
cultivation pool
solar panel
illumination
greenhouse
transparent tube
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Application number
CN201510513083.9A
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Chinese (zh)
Inventor
高志刚
高志峰
宣卫华
高楠
姜冰冰
汪世军
王海宇
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DONGTAI CITY CIBAINIAN BIOLOGICAL ENGINEERING Co Ltd
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DONGTAI CITY CIBAINIAN BIOLOGICAL ENGINEERING Co Ltd
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Priority to CN201510513083.9A priority Critical patent/CN105039138A/en
Publication of CN105039138A publication Critical patent/CN105039138A/en
Pending legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02Photobioreactors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/18Open ponds; Greenhouse type or underground installations
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06Nozzles; Sprayers; Spargers; Diffusers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M43/00Combinations of bioreactors or fermenters with other apparatus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12Unicellular algae; Culture media therefor

Abstract

The invention discloses a microalgae culture system with solar cell panels and a culture method thereof. The microalgae culture system with solar cell panels comprises a greenhouse, a culture tank and an inflator, wherein the upper part of the greenhouse is provided with solar cell panels which are arranged at intervals; the inflator is arranged on the outer side of the bottom of the culture tank and fixedly connected with the outer wall of the bottom of the culture tank; the culture tank is arranged inside the greenhouse; the culture tank is provided with a first liquid inlet and a first liquid outlet; the transparent pipe is provided with a second liquid inlet and a second liquid outlet; a light compensator is arranged in the culture tank; and the first liquid inlet and first liquid outlet are arranged on the outer wall of the bottom of the culture tank. The microalgae culture system has the characteristics of simple structure and favorable culture biological effect, and is convenient to use and easy to clean.

Description

With both culturing microalgae system and the cultural method thereof of solar panel
Technical field
The present invention relates to unicellular algae cultural technique field, particularly a kind of both culturing microalgae system with solar panel and cultural method thereof.
Background technology
Along with the raising of expanding economy and people's living standard, to quality of life and healthy requirement more and more higher, wherein get up to the demand of healthcare products also more.Spirulina is a kind of biology very useful to human body.Along with the increase to spirulina demand, the device and utensil demand cultivating spirulina is also got more and more.
Spirulina contains multiple beneficial growth in humans and healthy aquatic bacterium algae, and it eats and pharmaceutical use is more and more subject to social favor.Thus, in recent years spirulina propagate industry fast development artificially.In known cultural technique, the method that many employings pond is supported carries out suitability for industrialized production, its concrete grammar is: the place that selection and comparison is suitable, build multiple rectangle pond by laying bricks or stones, and baffle wall and the current striking gear that water stream channel is left at two ends is set on the length direction in the middle of pond, in breeding process, make culturing liquid together with algae strain slowly flow circuit, be beneficial to the growth and breeding of spirulina.Meanwhile, in breeding process, make cultivation together with algae strain slowly flow circuit, be beneficial to the growth and breeding of spirulina.Meanwhile, in breeding process, general employing supplements the necessary carbon source fertilizer of growth of spirulina platensis to the mode of injecting carbon dioxide gas in pond.At present, in culturing pool, execute release pipeline is established in the many employings of carbon dioxide injection gas method at pond base fabric, the carbon dioxide with certain pressure is discharged in culturing liquid lentamente, and dissolves by the solvability of carbonic acid gas self.But, when carbonic acid gas bubble during ascension, only has bubble surface and culturing liquid contact lysis in culturing liquid, and its solubleness and dissolution rate limited, a large amount of carbon dioxides rises to fluid surface, enters in air, carbon dioxide utilization ratio is very low on the one hand, causes the waste of resource; Turn increase the carbon dioxide content in air simultaneously, cause the Greenhouse effect of surrounding environment, and cause environmental pollution, bring impact to the life of people.
In order to improve prior art, people have carried out long-term exploration, propose various solution.Such as, Chinese patent literature discloses a kind of unicellular algae culture apparatus [application number: CN201320731353.X], its composition comprises: underframe, described underframe connects greenhouse, described greenhouse has interlayer, and described interlayer is built with electrical heating wire, and described electrical heating wire overcoat has water jacket, described electrical heating wire connects temperature regulator, and described temperature regulator connects store battery or external power.The side in described greenhouse connects water inlet pipe, described water inlet pipe connects strainer, described strainer connects water source, the opposite side in described greenhouse connects into nutritive medium pipe, described enter the position that nutritive medium pipe connects described greenhouse ultraviolet germicidal lamp is housed, the top in described greenhouse connects escape pipe, and the bottom in described greenhouse has unicellular algae mouth.
Although such scheme to some extent solves the deficiencies in the prior art, at present, a kind of the both culturing microalgae system with solar panel and cultural method thereof of energy-conserving and environment-protective is lacked.
Summary of the invention
The object of this invention is to provide a kind of the both culturing microalgae system with solar panel and cultural method thereof of energy-conserving and environment-protective.
In order to realize above-mentioned technical problem, technical scheme provided by the invention is: the invention provides a kind of described both culturing microalgae system with solar panel and comprise greenhouse, pump, cultivation pool and inflation mechanism;
The top in described greenhouse is provided with spaced solar panel, described inflation mechanism be arranged on cultivation pool bottom outside and with cultivation pool bottom outer wall Joint;
Described cultivation pool is arranged on the inside in greenhouse, described cultivation pool is provided with the first fluid inlet and the second liquid outlet, described transparent tube is provided with the second fluid inlet and the second liquid outlet, is provided with light aid in described cultivation pool; Described first fluid inlet and the first liquid outlet are arranged in the bottom outer wall of cultivation pool;
Be provided with the nutrient solution cultivating energy algae in described cultivation pool, the second fluid inlet of described transparent tube is connected with the first liquid outlet of cultivation pool by pump; Second liquid outlet of described transparent tube is connected with the first fluid inlet of cultivation pool;
Described light aid be arranged on cultivation pool bottom and with the inwall Joint of cultivation pool.
Further, described inflation mechanism is made up of air pump, tracheae and ventilative ring, and described air pump, tracheae are connected with ventilative ring.
Further, described light aid is colored led lamp.
Further, the culture-liquid temp in cultivation pool remains between 20-40 DEG C.
Further, the bottom in described greenhouse is provided with bottom support frame, and the side of bottom support frame is provided with the liquid crystal display for showing incubator correlation parameter.
Further, the order number of described porous solid ceramic filtering net is 200-360 order.
Further, the order number of described porous solid ceramic filtering net is 200-360 order.
Further, the bottom in described greenhouse is provided with bottom support frame, and the side of bottom support frame is provided with the liquid crystal display for showing incubator correlation parameter.
The cultural method of the both culturing microalgae system with solar panel of the present invention, comprises the steps:
(1) in intensity of illumination lower than 300 μm of ol/m 2during/s, cultivate spirulina;
(2) the general illumination of the cultivation pool under solar panel is at 4-6Wlux, and outdoor illumination is 10W, and every day, growth velocity was 0.1, and adopting transparent tube to cultivate speed is its 2-3 times;
(3) overcast and rainy not enough with illumination during night, the colored led lamp in glass sunlight house can be utilized to supplement light source, allow algae can 24 hours growth, when illumination deficiency, nutrient solution, by entering cultivation pool after transparent tube, makes the algae in transparent tube to obtain enough illumination.
Beneficial effect: the present invention makes full use of sunlight, save energy, environmental protection and economy, the feature that structure is simple, easy to use, cultivation biological effect is good.The Collocation cultivation of sun power make use of new forms of energy, also reduces the aquaculture cost of enterprise, and colored light sources also improves the quality of product.Relative to prior art, tool of the present invention has the following advantages:
(1) the present invention is provided with solar panel on top, greenhouse, it is the effect in order to play shading storage power when illumination is too strong, because illumination is too strong to the unfavorable suppression algal grown of algal grown, when illumination deficiency time, the energy that at this time solar panels store can provide the energy for light-supplementing system.
(2) the general illumination of the great Chi under sunlight board is at 4-6Wlux, outdoor illumination is 10W, every day, growth velocity was about 0.1, adopting pipeline to cultivate speed is about its 2-3 times, if adopt the solar panels of 2/3rds, the growth of great Chi by reduction by 2/3rds, and supplements cultivation with pipeline, and growth velocity will be much higher than original closed cultivation.
(3) during overcast and rainy and night, illumination is not enough, and the colored led lamp in glass sunlight house can be utilized to supplement light source, allows algae can 24 hours growth, improves the yield and quality of algae.When illumination deficiency, nutrient solution is looped around the top in greenhouse by transparent tube, and finally enters cultivation pool, and it is more that the algae in such transparent tube can obtain illumination, makes growth better, the cycle time of algae culture.
(4) the combination cultivation of the solid of plane can make full use of limited soil.Utilization rate of carbon dioxide can be improved fully, also can solve the emission problem of some enterprise's carbonic acid gas, the covert production capacity improving these enterprises.
Accompanying drawing explanation
Fig. 1 is schematic diagram of the present invention;
Wherein: 1 greenhouse, 2 cultivation pools, 3 inflation mechanisms, 4 solar panels, 5 first fluid inlets, 6 first liquid outlets, 7 pumps, 8 light aids, 9 transparent tube, 10 nutrient solutions.
Embodiment
In order to illustrate technical scheme of the present invention and technical purpose, below in conjunction with figure and embodiment, the present invention is described further.
Embodiment 1
As shown in Figure 1, the invention provides a kind of described both culturing microalgae system with solar panel and comprise greenhouse 1, pump 7, cultivation pool 2 and inflation mechanism 3;
The top in described greenhouse 1 is provided with spaced solar panel 4, described inflation mechanism 3 be arranged on cultivation pool 2 bottom outside and with cultivation pool 2 bottom outer wall Joint;
Described cultivation pool 2 is arranged on the inside in greenhouse 1, described cultivation pool is provided with the first fluid inlet 5 and the second liquid outlet 6, described transparent tube 9 is provided with the second fluid inlet and the second liquid outlet, is provided with light aid 8 in described cultivation pool 2; Described first fluid inlet 5 and the first liquid outlet 6 are arranged in the bottom outer wall of cultivation pool 2;
Be provided with the nutrient solution 10 cultivating energy algae in described cultivation pool 2, the second fluid inlet of described transparent tube 9 is connected with the first liquid outlet of cultivation pool 2 by pump 7; Second liquid outlet of described transparent tube 9 is connected with the first fluid inlet of cultivation pool 2; Described pump 7 provides power, makes nutrient solution 10 can enter transparent tube 9, and micro-like this algae can arrive sufficient sunlight, growth faster, and the cycle of cultivating system shortens.
Described light aid 8 be arranged on cultivation pool 2 bottom and with the inwall Joint of cultivation pool 2.
Described inflation mechanism 3 is made up of air pump, tracheae and ventilative ring, and described air pump, tracheae are connected with ventilative ring.
Described light aid 8 is colored led lamp.
Nutrient solution 10 temperature in cultivation pool 2 remains between 20-40 DEG C.
The bottom in described greenhouse 1 is provided with bottom support frame, and the side of bottom support frame is provided with the liquid crystal display for showing incubator correlation parameter.
The cultural method of the both culturing microalgae system with solar panel of the present invention, comprises the steps:
(1) in intensity of illumination lower than 300 μm of ol/m 2during/s, cultivate spirulina;
(2) the general illumination of the cultivation pool under solar panel is at 4-6Wlux, and outdoor illumination is 10W, and every day, growth velocity was 0.1, and adopting transparent tube to cultivate speed is its 2-3 times;
(3) overcast and rainy not enough with illumination during night, the colored led lamp in glass sunlight house can be utilized to supplement light source, allow algae can 24 hours growth, when illumination deficiency, nutrient solution, by entering cultivation pool after transparent tube, makes the algae in transparent tube to obtain enough illumination.
The present invention makes full use of sunlight, save energy, environmental protection and economy, the feature that structure is simple, easy to use, cultivation biological effect is good.The Collocation cultivation of sun power make use of new forms of energy, also reduces the aquaculture cost of enterprise, and colored light sources also improves the quality of product.Relative to prior art, tool of the present invention has the following advantages:
(1) the present invention is provided with solar panel 4 on top, greenhouse 1, it is the effect in order to play shading storage power when illumination is too strong, because illumination is too strong to the unfavorable suppression algal grown of algal grown, when illumination deficiency time, the energy that at this time solar panel 4 stores can provide the energy for light-supplementing system.
In intensity of illumination lower than 300 μm of ol/m 2during/s, the biomass of spirulina presents ascendant trend along with the enhancing of intensity of illumination, when intensity of illumination is 450 μm of ol/m 2during/s, frustule mortality, presents white.The too high meeting of light intensity significantly suppresses biological growth and CO 2fixed rate.At 75 μm of ol/m 2under the intensity of illumination of/s, there is an obvious lag phase in early growth period.With 75 μm of ol/m 2/ s compares, 150 μm of ol/m 2/ s and 300 μm ol/m 2under/s intensity of illumination, frustule growth is in earlier stage closely similar, and stage finds 300 μm of ol/m 2the illumination of/s is more favourable to frustule growth, and along with the accumulation of cellular biomass, this advantage is more remarkable.
(2) the general illumination of great Chi under solar panel 4 is at 4-6Wlux, outdoor illumination is 10W, every day, growth velocity was about 0.1, adopting pipeline to cultivate speed is about its 2-3 times, if adopt the solar panel 4 of 2/3rds, the growth of great Chi by reduction by 2/3rds, and supplements cultivation with pipeline, and growth velocity will be much higher than original closed cultivation.
(3) during overcast and rainy and night, illumination is not enough, and the colored led lamp in glass sunlight house can be utilized to supplement light source, allows algae can 24 hours growth, improves the yield and quality of algae.When illumination deficiency, nutrient solution 10 is looped around the top in greenhouse 1 by transparent tube 9, and finally enters cultivation pool 2, and it is more that the algae in such transparent tube 9 can obtain illumination, makes growth better, the cycle time of algae culture.
(4) the combination cultivation of the solid of plane can make full use of limited soil.Utilization rate of carbon dioxide can be improved fully, also can solve the emission problem of some enterprise's carbonic acid gas, the covert production capacity improving these enterprises.
Although more employ the terms such as greenhouse 1, cultivation pool 2, biological collection device 3, inflation mechanism 3, solar panel 4, fluid inlet 5, liquid outlet 6, pump 7, light aid 8, transparent tube 9, nutrient solution 10 herein, do not get rid of the possibility using other term.These terms are used to be only used to describe and explain essence of the present invention more easily; The restriction that they are construed to any one additional is all contrary with spirit of the present invention.
More than show and describe ultimate principle of the present invention, principal character and advantage of the present invention.The technician of the industry should understand; the present invention is not restricted to the described embodiments; what describe in above-described embodiment and specification sheets just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and application claims protection domain is defined by appending claims, specification sheets and equivalent thereof.

Claims (6)

1. with a both culturing microalgae system for solar panel, it is characterized in that: the described both culturing microalgae system with solar panel comprises greenhouse, pump, cultivation pool and inflation mechanism;
The top in described greenhouse is provided with spaced solar panel, described inflation mechanism be arranged on cultivation pool bottom outside and with cultivation pool bottom outer wall Joint;
Described cultivation pool is arranged on the inside in greenhouse, described cultivation pool is provided with the first fluid inlet and the second liquid outlet, described transparent tube is provided with the second fluid inlet and the second liquid outlet, is provided with light aid in described cultivation pool; Described first fluid inlet and the first liquid outlet are arranged in the bottom outer wall of cultivation pool;
Be provided with the nutrient solution cultivating energy algae in described cultivation pool, the second fluid inlet of described transparent tube is connected with the first liquid outlet of cultivation pool by pump; Second liquid outlet of described transparent tube is connected with the first fluid inlet of cultivation pool;
Described light aid be arranged on cultivation pool bottom and with the inwall Joint of cultivation pool.
2. the both culturing microalgae system with solar panel according to claim 1, is characterized in that: described inflation mechanism is made up of air pump, tracheae and ventilative ring, and described air pump, tracheae are connected with ventilative ring.
3. the both culturing microalgae system with solar panel according to claim 1, is characterized in that: described light aid is colored led lamp.
4. the both culturing microalgae system with solar panel according to claim 1, is characterized in that: the culture-liquid temp in cultivation pool remains between 20-40 DEG C.
5. the both culturing microalgae system with solar panel according to claim 1, is characterized in that: the bottom in described greenhouse is provided with bottom support frame, the side of bottom support frame is provided with the liquid crystal display for showing incubator correlation parameter.
6. the cultural method of the both culturing microalgae system with solar panel described in any one of claim 1 to 5, is characterized in that comprising the steps:
(1) in intensity of illumination lower than 300 μm of ol/m 2during/s, cultivate spirulina;
(2) the general illumination of the cultivation pool under solar panel is at 4-6Wlux, and outdoor illumination is 10W, and every day, growth velocity was 0.1, and adopting transparent tube to cultivate speed is its 2-3 times;
(3) overcast and rainy not enough with illumination during night, the colored led lamp in glass sunlight house can be utilized to supplement light source, allow algae can 24 hours growth, when illumination deficiency, nutrient solution, by entering cultivation pool after transparent tube, makes the algae in transparent tube to obtain enough illumination.
CN201510513083.9A 2015-08-19 2015-08-19 Microalgae culture system with solar cell panels and culture method thereof Pending CN105039138A (en)

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CN105684880A (en) * 2016-02-16 2016-06-22 珀莱雅化妆品股份有限公司 Culture method for improving mycosporine-like amino acid content in porphyra umbilicalis
CN110713919A (en) * 2019-11-04 2020-01-21 中国海洋大学 Marine algae culture device and application thereof

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CN110713919A (en) * 2019-11-04 2020-01-21 中国海洋大学 Marine algae culture device and application thereof

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Application publication date: 20151111