CN102575209B - Diphasic algal culture system - Google Patents
Diphasic algal culture system Download PDFInfo
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- CN102575209B CN102575209B CN201080032717.4A CN201080032717A CN102575209B CN 102575209 B CN102575209 B CN 102575209B CN 201080032717 A CN201080032717 A CN 201080032717A CN 102575209 B CN102575209 B CN 102575209B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M43/00—Combinations of bioreactors or fermenters with other apparatus
- C12M43/06—Photobioreactors combined with devices or plants for gas production different from a bioreactor of fermenter
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M43/00—Combinations of bioreactors or fermenters with other apparatus
- C12M43/02—Bioreactors or fermenters combined with devices for liquid fuel extraction; Biorefineries
Abstract
The present invention relates to, for producing at least biodiesel and/or hydrogen and/or the photo-bioreactor system of methane, particularly relate to a kind of two-phase photo-bioreactor system.
Description
Technical field
The present invention relates to bioreactor process, more particularly to for being produced biological bavin by biomass
Oil and/or the bioreactor method of hydrogen and/or methane, more particularly relate to be given birth to by algae
Produce biodiesel and/or the two-phase bioreactor method of hydrogen and/or methane.
Background technology
Along with the price of Fossil fuel and oil rises, biomass produce biodiesel and/or hydrogen
Gas and/or methane and other fermented products be increasingly becoming alternative fuel product one is attractive
Selection.Along with Fossil fuel is depleted, alternative energy source will become a pass of industry and academia
Key research field.
Specifically, hydrogen be considered as a kind of clean and can the energy carrier of recirculation, and closing
The research initiative that abundant, effective, favourable and " green " of note hydrogen produces exists significantly to become
Gesture.It is believed that, it is strictly following it is contemplated that and develop as a kind of hydrogen substituting energy carrier
Substitute more likely in one.Biomass are used to provide one to use in the production of hydrogen
In hydrogen produce " green " solution, it is desirable to this solution can optimised and research and develop, to carry
For a kind of mode for providing economic and profitable hydrogen to supply.Additionally, from organic waste materials with
And other Renewable resources produce hydrogen and are considered to be preferable over from cereal crops production hydrogen, this is because:
Although the hydrogen yield of cereal crops (such as Semen Maydis and Semen Tritici aestivi) is relatively high, but by biological hydrogen
Produce the risk using cereal crops to there is aggravation world food deposit in reactor.
Similarly, in energy production required in modern society, biodiesel quickly becomes biography
A kind of feasible substitute of system Fossil fuel.Biodiesel is commonly referred to as by biomaterial itself
The diesel fuel that (including long-chain alkyl groups) makes.Should include auto industry or transportation industry
The use of biodiesel is especially considered in.
Life employed in the production of biodiesel and/or hydrogen and/or methane and/or other fuel
Thing reactor can be specifically designed for staying in the biomass type used in this system.
The bioreactor using algal biomass is typically bioreactor form.Photo bio is anti-
Answer utensil be provided with energy be cultured to the light source of reactor assembly, algae or other phototrophs and
Support in this reactor assembly.Generally, biodiesel is produced at open lead from algal biomass
(raceway) formula photo-bioreactor system is carried out.The manufacture of these systems known and maintenance
It is expensive, because they require special purpose reactor container, chiller, mixing arrangement and gas
Control device.
Known compared to more conventional open lead system, for the Guan Bi of algae bio diesel production
Tubular light bioreactor system promote algal biomass produce higher levels of process control and
Higher speed.Generally these bioreactors are only with aerobic photoautotrophy algae, thus such as
Fruit imagination needs artificial lighting when non-daylight produces period.
Accordingly, it would be desirable to a kind of cheap photo-bioreactor system, wherein this bioreactor system
System can maintain in whole two-phase circulates in the case of need not artificial lighting and produce.
Goal of the invention
It is an object of the invention to provide a kind of bioreactor for producing at least biodiesel
System, particularly provides a kind of two-phase photoproduction for producing biodiesel and/or hydrogen and/or methane
Thing reactor assembly, the biomass wherein utilized are algae.
Summary of the invention
According to the first aspect of the invention, it is provided that a kind of two-phase for producing at least biodiesel
Photo-bioreactor system, this two-phase photo-bioreactor system includes:
One bioreactor container, it is divided into the first compartment and the second compartment；
One attachment means, it is connected between described first compartment and described second compartment so that
Described first compartment and described second compartment fluid communication；And
One biodiesel refining equipment, itself and described bioreactor reservoir.
Described two-phase photo-bioreactor system, farther includes to be positioned at this bioreactor container
In nutrient inflow entrance.
Described two-phase photo-bioreactor system, wherein said nutrient inflow entrance is connected to described
First compartment, to allow nutrient controllably to flow into described first compartment.
Described two-phase photo-bioreactor system, farther includes to be connected to described second compartment
Second nutrient inflow entrance, to allow nutrient controllably to flow into described second compartment.
Described two-phase photo-bioreactor system, wherein said nutrient includes nitrogenous and phosphorus chemical combination
Thing.
Described two-phase photo-bioreactor system, wherein said first compartment has algal cultures,
And breed for alga cells；And described second compartment has algal cell cultures, and
Produce for lipid.
Described two-phase photo-bioreactor system, wherein said attachment means is easy to the algae bred
Cell moves to described second compartment from described first compartment.
Described two-phase photo-bioreactor system, wherein said second compartment is via the second attachment means
Connect with described biodiesel refining equipment.
Described two-phase photo-bioreactor system, wherein said biodiesel refining equipment utilizes and comprises
Lipid in the alga cells from described second compartment produces biodiesel.
Described two-phase photo-bioreactor system, wherein by the combination of independently controlled the following
Or all the followings optimizes the alga cells breeding in described first compartment: set point temperatures,
Saturation irradiation level, nitrogen and phosphorus supply, carbon dioxide supply, salinity and PH.
Described two-phase photo-bioreactor system, wherein by optionally control from described first every
Between enter the nitrogen in described second compartment and phosphorus to promote that the lipid in described second compartment produces.
Described two-phase photo-bioreactor system, wherein by providing high level to described second compartment
Irradiation and/or high-caliber carbon dioxide promote that lipid in described second compartment is raw further
Produce.
Described two-phase photo-bioreactor system, wherein by optionally controlling from described second battalion
Nitrogen and the phosphorus of supporting thing entrance described second compartment of entrance promote that the lipid in described second compartment is raw
Produce.
Described two-phase photo-bioreactor system, wherein by the combination of independently controlled the following
Or all the followings minimize the alga cells in described second compartment and breed: set point temperature
Degree；Saturation irradiation level, nitrogen and phosphorus supply, carbon dioxide supply, salinity and PH.
Described two-phase photo-bioreactor system, wherein said bioreactor container is tubulose
's.
Described two-phase photo-bioreactor system, wherein said bioreactor includes that uv-resistant is moulded
Material material, preferably transparent PVC or makrolon material, have preferably less than 4.0mm
Pipe thickness.
Described two-phase photo-bioreactor system, farther includes to be connected via the 3rd attachment means
Anaerobism fluidized particles bed reactor (anaerobic to described biodiesel refining equipment
Fluidized granular bed reactor), described 3rd attachment means is easy to algae bio
Matter and/or glycerol move to the reaction of described anaerobism fluidized particles bed from described diesel oil refining equipment
Device, wherein said algal biomass is fermented by anaerobic bacteria consortium at least in part, is caused at least
The generation of hydrogen.
Described two-phase photo-bioreactor system, farther includes gas collecting device, to gather
Produced during the dark anaerobic fermentation of the alga cells of described anaerobism fluidized particles bed inside reactor
Described at least hydrogen.
Described two-phase photo-bioreactor system, farther includes TRT, described TRT
Hydrogen is utilized to generate electricity.
Described two-phase photo-bioreactor system, wherein said anaerobism fluidized particles bed reactor passes through
The anaerobism of discarded algal biomass secretly ferment produce further methane, ethanol, acetate, the third three
At least one in alcohol and volatile fatty acid.
Described two-phase photo-bioreactor system, wherein said anaerobism fluidized particles bed reactor enters one
Step includes the 4th attachment means, in order to acetate, glycerol and/or volatile fatty acid move
To storage box.
Described two-phase photo-bioreactor system, wherein said storage box and described bioreactor
Reservoir, preferably connects with described first compartment, thus provides acetic acid for described alga cells
Salt, glycerol and/or the carbon substrate of volatile fatty acid form, aerobic to support during dark phase
Heterotrophism algae reproduction and lipid are assembled.
Described two-phase photo-bioreactor system, bioreactor container described in each of which all wraps
Include a condenser.
Described two-phase photo-bioreactor system, wherein said bioreactor container includes one
Gas source systems, described gas source systems has a gas compressor, a heat exchanger and many
Individual gas sparger, wherein said gas compressor provides a gas source, and described heat exchanger is assisted
Help the temperature adjusted within described bioreactor container, and described gas sparger is used as gas
Body inlet device, in order to described first bioreactor and described second bioreactor
Bubbling.
In described two-phase photo-bioreactor system, wherein said first compartment and described second compartment
Each include a gas source systems, described gas source systems has a gas compression
Machine, a heat exchanger and multiple gas sparger, wherein said gas compressor provides one
Gas source, described heat exchanger assists to adjust described first compartment and the temperature of described second compartment
Degree, and described gas sparger is as gas inlet device, in order to described first photo bio is anti-
Answer device and the bubbling of described second bioreactor.
Described two-phase photo-bioreactor system, wherein provides to described the from described gas compressor
The described gas source of one compartment and described second compartment is the air of carbon dioxide-enriched.
Described two-phase photo-bioreactor system, wherein provides to described the from described gas compressor
The described gas source of one compartment and described second compartment is carbon dioxide.
Described two-phase photo-bioreactor system, wherein produced hydrogen and methane can be used for electricity
Power produces.
According to the second aspect of the invention, it is provided that a kind of two-phase for producing at least biodiesel
Photo-bioreactor system, described two-phase photo-bioreactor system includes:
First bioreactor container and the second bioreactor container, described first photo bio
Reactor vessel has algal cultures, and breeds for alga cells, and described second photo bio is anti-
Answer device container to have algal cell cultures, and produce for algae；
One the first attachment means, it makes described first bioreactor container and described second light
Bioreactor vessel connects with communicating with each other, it is simple to the alga cells bred is from described first light
Bioreactor vessel moves to described second bioreactor container；
One biodiesel refining equipment, for producing biology from the lipid being included in alga cells
Diesel oil；And
One the second attachment means, described second bioreactor container is connected to described life by it
Thing diesel oil refining equipment, it is simple to alga cells moves to described life from described second bioreactor
Thing diesel oil refining equipment.
Described two-phase photo-bioreactor system, farther includes to be positioned at described first optical-biological reaction
Nutrient inflow entrance in device container, anti-to allow nutrient controllably to flow to described first photo bio
Answer device container.
Described two-phase photo-bioreactor system, farther includes to be positioned at described second optical-biological reaction
The second nutrient inflow entrance in device container, to allow nutrient controllably to flow to described second photoproduction
Thing reactor vessel.
Described two-phase photo-bioreactor system, wherein said nutrient includes the change comprising nitrogen and phosphorus
Compound.
Described two-phase photo-bioreactor system, wherein by the combination of independently controlled the following
Or the alga cells that all the followings optimize in described first bioreactor container is numerous
Grow: set point temperatures, saturation irradiation level, nitrogen and phosphorus supply, carbon dioxide supply, salinity and
PH。
Described two-phase photo-bioreactor system, wherein by optionally controlling from described first light
Bioreactor vessel enters the nitrogen in described second bioreactor container and phosphorus promotes institute
State the lipid in the second bioreactor container to produce.
Described two-phase photo-bioreactor system, wherein by holding for described second bioreactor
Device provides high-caliber irradiation and/or high-caliber carbon dioxide to promote described second light further
Lipid in bioreactor vessel produces.
Described two-phase photo-bioreactor system, wherein by optionally controlling from described second battalion
Support the nitrogen in the thing entrance described second bioreactor container of entrance and phosphorus promotes described second
Lipid in bioreactor container produces.
Described two-phase photo-bioreactor system, wherein by the combination of independently controlled the following
Or all the followings minimize the alga cells in described second bioreactor container
Breeding: set point temperatures, saturation irradiation level, nitrogen and phosphorus supply, carbon dioxide supply, salinity
And PH.
Described two-phase bioreactor container, wherein said first bioreactor container and institute
Stating the second bioreactor container is tubulose.
Described two-phase photo-bioreactor system, wherein said bioreactor container includes resisting
UV plastic material, preferably transparent PVC or makrolon material, have less than 4.0mm's
Preferably pipe thickness.
Described two-phase photo-bioreactor system, farther includes to be connected via the 3rd attachment means
To the anaerobism fluidized particles bed reactor of described biodiesel refining equipment, described 3rd attachment means
It is easy to algal biomass and/or glycerol move to described anaerobism from described diesel oil refining equipment and fluidize
Grain bed reactor, wherein said algal biomass is sent out by anaerobic bacteria consortium at least in part
Ferment, causes the generation of at least hydrogen.
Described two-phase photo-bioreactor system, farther includes gas collecting device, to gather
Produced during the dark anaerobic fermentation of the alga cells of described anaerobism fluidized particles bed inside reactor
Described at least hydrogen.
Described two-phase photo-bioreactor system, farther includes TRT, described TRT
Hydrogen is utilized to generate electricity.
Described two-phase photo-bioreactor system, wherein said anaerobism fluidized particles bed reactor passes through
The anaerobism of discarded algal biomass secretly ferment produce further methane, ethanol, acetate, the third three
At least one in alcohol and volatile fatty acid.
Described two-phase photo-bioreactor system, wherein said anaerobism fluidized particles bed reactor enters one
Step includes the 4th attachment means, in order to acetate, glycerol and/or volatile fatty acid move
To storage box.
Described two-phase photo-bioreactor system, wherein said storage box is anti-with described first photo bio
Answer device container and/or described second bioreactor reservoir, thus be described alga cells
There is provided acetate, glycerol and/or the carbon substrate of volatile fatty acid form, with during dark phase
Support that the breeding of aerobic heterotrophism alga cells and lipid are assembled.
Described two-phase photo-bioreactor system, wherein said first bioreactor container and institute
State each in the second bioreactor container and include a condenser.
Described two-phase photo-bioreactor system, wherein said first bioreactor container and institute
State each in the second bioreactor container and include a gas source systems, described gas
Origin system has a gas compressor, a heat exchanger and multiple gas sparger, Qi Zhongsuo
Stating gas compressor and provide a gas source, described heat exchanger assists to adjust described first photo bio
Temperature within reactor vessel and described second bioreactor container, and described gas drum
Bubble device is used as gas inlet device, in order to described first bioreactor container and described second
The bubbling of bioreactor container.
Described two-phase photo-bioreactor system, wherein provides to described the from described gas compressor
The described gas source of one bioreactor container and described second bioreactor container is rich
The air of collection carbon dioxide.
Described two-phase photo-bioreactor system, wherein provides to described from described gas compressor
The described gas source of the first bioreactor container and described second bioreactor container is
Carbon dioxide.
Described two-phase bioreactor container, wherein produced hydrogen and methane can be used for described
The production of electric power.
Accompanying drawing explanation
To only pass through embodiment and embodiment of the present invention will be described in reference to the drawings below, accompanying drawing
Show the schematically showing an of embodiment of the two-phase bioreactor according to the present invention.
Detailed description of the invention
Two-phase photo-bioreactor system 10 includes the first tube like transparent light being connected to each other
Bioreactor vessel 12 and the second tube like transparent bioreactor container 14.
First tube like transparent bioreactor container 12 operationally comprises algal cultures, and
And it is used for growth and the breeding of this algal cultures.Second tube like transparent bioreactor container
14 comprise the algal cell cultures produced for lipid.With the first emission discharger 16 form
The first attachment means connect the first and second tube like transparent bioreactor containers 12,14,
So that by the alga cells of flourish from the first tube like transparent bioreactor container 12
It is transferred to the second tube like transparent bioreactor container 14.First and second tubular lights are biological anti-
Each in device container 12,14 is answered to comprise a nutrient inflow entrance 18, to allow to pass through
Effusion meter (not shown) or variable pump (not shown) is utilized to make nutrient from nutrient tank 15
Controllably flow to bioreactor container 12,14.
First bioreactor container 12 is supplied with Nutrient medium, and this Nutrient medium is enriched with
Promote nitrogen and the phosphorus of the concentration level of cell proliferation and the speed of growth.Thus, alga cells breeding and
Growth occurs in the first bioreactor container 12, wherein supplies to bioreactor appearance
Most of nitrogen of device 12 and phosphorus are consumed by algae culture.Therefore, the only nitrogen of surplus and phosphorus warp
It is transferred to the second bioreactor 14 by the first emission discharger 16.In the second photo bio
The nitrogen of the low concentration received in reactor vessel and phosphorus provide insufficient support to maintain algae thin
Born of the same parents divide, cause with the first bioreactor container 12 in occur breeding and growth phase ratio,
Create breeding and the growth of low relative levels.But, with the first bioreactor container 12
In algae lipid produce and compare, the second bioreactor container 14 experienced by algae at a high speed
Lipoidis produces.In response to low nitrogen and phosphorus concentration, at unrestricted irradiation and from gas source
In the case of the unconfined carbon dioxide of system 20 supply, in the second bioreactor 14
Occurring lipid to produce and corresponding lipid is assembled, gas source systems 20 allows selected gas quilt
Introduce in the first and second bioreactor containers 12,14 or the two.
Gas source systems 20 includes: a gas compressor 22 for gas compression；One use
The thermoregulator heat exchanger of operation 24 in described gas；And a gas sparger 26,
It is easy to into and through for gas sparging the first and second bioreactor containers 12,14.
After nitrogen in ruing out of the second bioreactor container 14 completely and phosphorus, in algae chloroplast
Lipid be converted into depot lipid and oil.Chloroplast subjected to size and reduces and photosynthetic rate decline
Level is maintained to cell.Once chloroplast subjected to size reduction, and lipid is kept with oil storing body
And it is the most depleted.Alga cells can be maintained at this state with high density within a couple of days.Relative to phase
As irradiation load, the temperature in the first and second bioreactor containers 12,14 can have difference
Not by via the temperature of gas sparger supply to the air-flow of single bioreactor and stream
Speed is controlled.This air-flow is made up of air, is preferably enriched with CO2Air.
In photosynthesis saturation irradiation level, unrestricted nitrogen supply and unrestricted phosphorus supply
With the CO improved2Under supply status, facilitate the first optical-biological reaction of the optimal velocity of cell proliferation
The set point temperatures of device container 12 can be controlled by gas source systems 20.Similarly, in light cooperation
With saturation irradiation level, low nitrogen concentration and low phosphorus concentration and the CO of raising2Under supply status,
The lipid facilitating optimal velocity produces setting of the second bioreactor container 14 with lipid gathering
Set point temperature also can be controlled by air-delivery system.
Except photosynthesis saturation irradiation level, the temperature of rising, the CO of rising2Level, low
The positive influences that lipid in alga cells is produced by nitrogen concentration and low phosphorus concentration and lipid is assembled,
Other factors (such as salinity and PH) can be additionally used in and promote the lipid in alga cells to produce and fat
Class is assembled.Thus, in the second bioreactor container 14, can and individually control simultaneously under
The combination of column processing factor produce strengthening the algae lipid under photosynthesis saturation irradiation level and
Lipid is assembled: CO2Concentration, nitrogen and phosphorus concentration, temperature, salinity and PH.Similarly, for
One bioreactor container 12, the positive influences of the combination of following factors can be used for promoting further
Make or actively increase the two-forty that alga cells produces: photosynthesis saturation irradiation level, rising
Temperature, the CO of rising2Level, N and P concentration, alga cells produce, to such as salinity and PH
The control of factor.Thus, in this first bioreactor, the combination of lower column processing factor
Can concurrently and independently be controlled, raw to strengthen the alga cells under photosynthesis saturation irradiation level
Produce: CO2Concentration, N and P concentration, temperature, salinity and PH.Via via gravity flow through collection
By alga cells from the first emission discharger 16 being connected to the second bioreactor container 14
Extract and enter biodiesel refining equipment 25.Biodiesel refining equipment 25 includes water channel pond
(raceway pond) (not shown), before alga cells is processed as biodiesel, water channel
Pond is used as the temporary storage facilities of alga cells.The biodiesel produced can be from biodiesel refine
Equipment is sent to desired locations via biodiesel conduit 27.
But the design of single tube like transparent bioreactor container 12,14 is based preferably on not
It is limited to fourth dimension: highly 5.1m；Diameter 0.1m；Biological respinse volume 40L.Tubulose is saturating
Mingguang City's bioreactor vessel 12,14 is made up, such as of the uvioresistant plastic material of low cost
Pipe thickness is less than transparent PVC or the makrolon material of 4.0mm.
Extend from the upper end 30 of the first and second bioreactors 12,14 is condenser 32.
Second bioreactor is held by the second attachment means of the second emission discharger 34 form
Device 14 is connected to biodiesel refining equipment 25, in order to alga cells is from the second optical-biological reaction
Device 14 moves to biodiesel refining equipment 25.Two-phase photo-bioreactor system 10 is further
Biological bavin it is connected to including the 3rd attachment means 38 via the 3rd emission discharger 38 form
The anaerobism fluidized particles bed reactor 28 of oil refinement equipment 25, the 3rd attachment means 38 is easy to
Algal biomass and/or glycerol move to anaerobism fluidized particles from biodiesel refining equipment 25
Bed reactor 28, wherein this algal biomass is fermented by anaerobic bacteria consortium at least in part,
Cause secretly fermenting via the anaerobism of discarded algal biomass and produce hydrogen, methane, ethanol, acetate,
Glycerol and volatile fatty acid.
Two-phase photo-bioreactor system 10, wherein anaerobism fluidized particles bed reactor 28 is further
Including the 4th attachment means of the 4th emission discharger 40 form, the 4th emission discharger 40
It is easy to mobile acetate, glycerol and/or volatile fatty acid to storage box 42, this storage box 42
It is connected with the first and/or second bioreactor container 12,14 via the second attachment means 44
Logical, thus provide acetate, glycerol and/or the carbon of volatile fatty acid form for alga cells
Substrate, thus during dark phase, support that aerobic heterotrophism algae reproduction and lipid are assembled.
In another embodiment of the present invention, each two-phase Photoreactor 10(one of them two
Phase Photoreactor 10 is made up of two bioreactor containers being connected in series) can be assembled into
One system, every 100 meters of this system is made up of 500 individual units, corresponding to per hectare 100000
Each two-phase bioreactor density of individual unit.In this another embodiment, about 0.5m
Space separate every a line Photoreactor.
It is positioned at the surface below this Photoreactor by covering with high reflecting material, promotes via two
Reflection in phase bioreactor 10 array reallocate solar irradiation reallocation, and promote
Photon is captured again by two-phase bioreactor 10.
The preferred density of 100000 unit of per hectare corresponds to per hectare 8000m3Total light
Bioreactor volume, per hectare 4000m3Alga cells produce and per hectare 4000m3
Lipid produce and assemble.After extracting lipid, treated algal biomass is together with glycerol
Be used as anaerobism fluidized particles bed bioreactor 28 for biological hydrogen, methane, ethanol
The raw material produced with volatile fatty acid (VFA), acetate is main VFA.
Biological hydrogen and ethanol are used as the fuel of generating, and are connected to TRT and (do not show
Go out).
It is used as substrate, with at dark period biological support by VFA produced by anaerobic biological reactor
Algal grown and lipid in reactor unit produce, thus allow circulate for continual 24 hours
Algae cell growth and lipid produce.The suitable operator scheme of anaerobic biological reactor is easy to institute
There is CO2Be converted to acetate.Thus by two-phase bioreactor 10 and anaerobism fluidized particles bed
The combined system that bioreactor collectively constitutes operates together as clean CO2Converge (sink).And mostly
Number carbon leaves this system as algae bio diesel oil.
Thus, it is provided that a kind of cheap two-phase photo-bioreactor system.
Claims (17)
1. for producing a two-phase photo-bioreactor system at least biodiesel, this two-phase
Photo-bioreactor system includes:
First bioreactor container and the second bioreactor container, wherein said first light
Bioreactor vessel has algae media, and breeds for alga cells；And described
Two bioreactor containers have alga cells culture medium, and produce for lipid；
One attachment means, it is connected to described first bioreactor container and described second light
Between bioreactor vessel so that described first bioreactor container and described second photoproduction
Thing reactor vessel connects, and wherein said attachment means is easy to the alga cells bred from described the
One bioreactor container moves to described second bioreactor container；
One biodiesel refining equipment being used for producing at least biodiesel, it is via one second
Attachment means and described second bioreactor reservoir, wherein said biodiesel refine sets
Standby utilization is included in and comes from the lipid in the alga cells of described second bioreactor container
Produce biodiesel；And
One anaerobism fluidized particles bed reactor, it is connected to described via the 3rd attachment means
Biodiesel refining equipment, wherein said 3rd attachment means is easy to algal biomass and/or the third three
Alcohol moves to described anaerobism fluidized particles bed reactor from described diesel oil refining equipment, in described anaerobism
Described in fluidized particles bed reactor, algal biomass is sent out by anaerobic bacteria consortium at least in part
Ferment, causes the generation of at least hydrogen,
Wherein said anaerobism fluidized particles bed reactor is secretly sent out by the anaerobism of discarded algal biomass
Ferment produces in methane, ethanol, acetate, glycerol and volatile fatty acid at least further
One, and
Wherein said anaerobism fluidized particles bed reactor farther includes the 4th attachment means, with
Be easy to acetate, glycerol and/or volatile fatty acid move to storage box, described storage box with
Described first bioreactor reservoir, thus for described alga cells provide acetate, third
Triol and/or the carbon substrate of volatile fatty acid form, to support during dark phase anaerobic fermentation
Oxygen heterotrophism algae reproduction and lipid are assembled.
Two-phase photo-bioreactor system the most according to claim 1, wherein said first
Bioreactor container and described second bioreactor container form single container.
Two-phase photo-bioreactor system the most according to claim 1, wherein said first
Bioreactor container and described second bioreactor container are separate.
4., according to the two-phase photo-bioreactor system according to any one of claim 1-3, enter
One step includes entering the nutrient inflow entrance in described first bioreactor container, as nutrition
The entrance of thing；And the second nutrient entered in described second bioreactor container flows into
Mouthful, to allow nutrient controllably to flow to described second bioreactor container.
Two-phase photo-bioreactor system the most according to claim 4, wherein said nutrition
Thing includes nitrogenous and phosphorus compound.
Two-phase photo-bioreactor system the most according to claim 1 and 2, wherein passes through
The combination of independently controlled the following or all the followings optimize described first photo bio
Alga cells breeding in reactor vessel: set point temperatures, saturation irradiation level, nitrogen and phosphorus supply
Should, carbon dioxide supply, salinity and pH.
Two-phase photo-bioreactor system the most according to claim 4, wherein by selecting
Property control from described first bioreactor container or described second nutrient inflow entrance enter institute
State the nitrogen in the second bioreactor container and phosphorus to promote that described second bioreactor holds
Lipid in device produces.
Two-phase photo-bioreactor system the most according to claim 7, wherein by institute
Stating the second bioreactor container provides high-caliber irradiation and/or high-caliber carbon dioxide
Promote that the lipid in described second bioreactor container produces further.
Two-phase photo-bioreactor system the most according to claim 1 and 2, wherein passes through
The combination of independently controlled the following or all the followings minimize described second photoproduction
Alga cells breeding in thing reactor vessel: set point temperatures, saturation irradiation level, nitrogen and phosphorus
Supply, carbon dioxide supply, salinity and pH.
Two-phase photo-bioreactor system the most according to claim 1 and 2, wherein said
First bioreactor container and described second bioreactor container are tubuloses.
11. two-phase photo-bioreactor systems according to claim 1 and 2, wherein said
The material of bioreactor includes uv-resistant plastic material.
12. two-phase photo-bioreactor systems according to claim 11, wherein said anti-
UV plastics are transparent PVC or makrolon material, described transparent PVC or makrolon material tool
There is the pipe thickness less than 4.0mm.
13. two-phase photo-bioreactor systems according to claim 1 and 2, wrap further
Include gas collecting device, to gather the alga cells at described anaerobism fluidized particles bed inside reactor
Dark anaerobic fermentation during produced hydrogen.
14. two-phase photo-bioreactor systems according to claim 13, farther include
TRT, described TRT utilizes hydrogen to generate electricity.
15. two-phase photo-bioreactor systems according to claim 1 and 2, each of which
Described bioreactor container all includes a condenser.
16. two-phase photo-bioreactor systems according to claim 1 and 2, it includes one
Individual gas source systems, described gas source systems have a gas compressor, a heat exchanger and
Multiple gas sparger, wherein said gas compressor provides a gas source, described heat exchanger
Assist to adjust the temperature within described bioreactor container, and described gas sparger is used as
Gas inlet device, in order to described first bioreactor and described second bioreactor
Bubbling.
17. two-phase photo-bioreactor systems according to claim 16, wherein from described
Gas compressor provides to described first bioreactor and described second bioreactor
Described gas source is air or the carbon dioxide of carbon dioxide-enriched.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ZA200904369 | 2009-06-23 | ||
| ZA2009/04369 | 2009-06-23 | ||
| PCT/IB2010/052824 WO2010150190A2 (en) | 2009-06-23 | 2010-06-22 | Diphasic algal culture system |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102575209A CN102575209A (en) | 2012-07-11 |
| CN102575209B true CN102575209B (en) | 2016-08-24 |
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| CN201080032717.4A Expired - Fee Related CN102575209B (en) | 2009-06-23 | 2010-06-22 | Diphasic algal culture system |
Country Status (5)
| Country | Link |
|---|---|
| CN (1) | CN102575209B (en) |
| AP (1) | AP3236A (en) |
| AU (1) | AU2010264093B2 (en) |
| WO (1) | WO2010150190A2 (en) |
| ZA (1) | ZA201200430B (en) |
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| CN105624026A (en) * | 2014-12-01 | 2016-06-01 | 中粮集团有限公司 | Low-carbon zero-emission circulating hydrogen preparing device |
| IT201600045987A1 (en) * | 2016-10-25 | 2018-04-25 | Ianni Giuseppe Di | Aquatic algae and terrestrial biomass in double anaerobic digestion |
| EP4163358A1 (en) * | 2021-10-07 | 2023-04-12 | Solmeyea Monoprosopi I.K.E. | Sustainably boosting carbon dioxide fixation for growing micro-algae |
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| IT1262502B (en) * | 1993-08-27 | 1996-06-28 | Consiglio Nazionale Ricerche | TUBULAR PHOTOBIOREACTOR PLANT FOR THE INDUSTRIAL CULTURE OF PHOTOSYNTHETIC MICROORGANISMS. |
| US6254775B1 (en) * | 1998-03-09 | 2001-07-03 | Mcelvaney James D. | Anaerobic digester system and method |
| US20050064577A1 (en) * | 2002-05-13 | 2005-03-24 | Isaac Berzin | Hydrogen production with photosynthetic organisms and from biomass derived therefrom |
| US6887692B2 (en) * | 2002-12-17 | 2005-05-03 | Gas Technology Institute | Method and apparatus for hydrogen production from organic wastes and manure |
| US8404004B2 (en) * | 2006-12-29 | 2013-03-26 | Genifuel Corporation | Process of producing oil from algae using biological rupturing |
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2010
- 2010-06-22 CN CN201080032717.4A patent/CN102575209B/en not_active Expired - Fee Related
- 2010-06-22 WO PCT/IB2010/052824 patent/WO2010150190A2/en active Application Filing
- 2010-06-22 AU AU2010264093A patent/AU2010264093B2/en not_active Ceased
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| Publication number | Publication date |
|---|---|
| AU2010264093A1 (en) | 2012-02-09 |
| AU2010264093B2 (en) | 2014-03-06 |
| CN102575209A (en) | 2012-07-11 |
| WO2010150190A2 (en) | 2010-12-29 |
| AP2012006098A0 (en) | 2012-02-29 |
| WO2010150190A3 (en) | 2011-04-07 |
| AP3236A (en) | 2015-04-30 |
| ZA201200430B (en) | 2014-05-28 |
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