CN105039139A - Microorganism culture membrane and microorganism culture recovery system - Google Patents
Microorganism culture membrane and microorganism culture recovery system Download PDFInfo
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- CN105039139A CN105039139A CN201510589827.5A CN201510589827A CN105039139A CN 105039139 A CN105039139 A CN 105039139A CN 201510589827 A CN201510589827 A CN 201510589827A CN 105039139 A CN105039139 A CN 105039139A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/20—Material Coatings
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/02—Membranes; Filters
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
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Abstract
The invention discloses a microorganism culture membrane and a microorganism culture recovery system including the culture membrane. The microorganism culture membrane is of a three-dimensional porous structure, and is made of a water-soluble polymer material which cannot be dissolved at a normal temperature and is dissolved when the temperature rises to 45-150 DEG C. The microorganism culture recovery system is composed of the microorganism culture membrane, a substrate, a liquid supply pipe arranged at the upper end of the substrate and a liquid collecting pipe arranged at the lower end of the substrate. The microorganism culture membrane is attached to the substrate and can be replaced. When the culture recovery system is used, the microorganism culture membrane of the three-dimensional porous structure has the high specific surface area, and the microorganism productive rate can be better increased. After culture is completed, microorganisms can be efficiently collected only through simple warming dissolution and filtration. After the dissolved culture membrane is subjected to centrifugal filtration, filter liquor can be used as a spinning solution to be recovered, the filter liquor is directly used for secondary forming processing of the microorganism culture membrane, and thus the microorganism culture membrane can be recycled. Due to the convertibility of the microorganism culture membrane, continuous industrialization production is easy, energy consumption is low, the productive rate is high, and the utilization rate is increased.
Description
Technical field
The present invention relates to a kind of microorganism culturing film and microorganism culturing recovery system.
Background technology
Microorganism is the life form the most widely that distributes, and is almost distributed to tellurian all regions, is widely used in multiple fields such as food, medicine, industry, agriculturals.Microorganism can utilize various organic compound, mineral substance, illumination etc. as the energy, clean and effective produce the target products such as lipid, protein, VITAMIN, polysaccharide and microbiotic.Due to advantages such as growth cycle is short, the high and low pollutions of productive rate, the present biotechnology being representative with genetically engineered, enzyme engineering, fermentation engineering, is flooded with the existence of microorganism everywhere.Along with problems such as the day by day exhausted of fossil energy and environmental pollutions, the range of application of microorganism is also in continuous expansion.Particularly obtain rich grease-contained microalgae biomass by micro-algae large scale culturing, form biofuel through extracting and transforming, be considered to solve bioenergy and produce and one of most important approach of carbon fixation and emission reduction.To micro-careless biofuel industry, the technical bottleneck of restriction micro-algae biofuel industrialized developments such as the cultural method that still there is micro-algae is perfect not, recovery process is loaded down with trivial details.
Current microorganism culturing mode has the open or closed microorganism culturing modes such as runway pool, duct type; No matter be the closed microorganism culturing modes such as the open microorganism culturing modes such as runway pool, or duct type, in improving productivity per unit and reducing costs, there is no breakthrough progress; New development is in recent years got up a kind of half dry type solid state rheology mode: to have the material of certain water-retentivity, such as filamentary material, as culture medium carrier, culture medium carrier hangs in a certain way, nutrient solution supplements from top to bottom, be stored in culture medium carrier (fiber) inner, the nutrient solution of culture medium carrier (fiber) internal reservoir permeates from inner exterior surface, for microorganism provides growth conditions; This method reduces energy consumption to a certain extent, improves productive rate, improves space availability ratio.But due to influence of gravity, this training method still also exists the technical bottleneck of feed flow inequality.
Microalgae recovery is the important step of micro-algae large-scale cultivation and industrial applications, and the separating technology optimizing algae is the key problem in technology reduced costs.But due to the singularity of micro-algae and nutrient solution thereof, traditional solid-liquid separation technique all cannot be directly used in microalgae recovery.Microalgae cell individuality little (2-40 μm), concentration low (0.5-2g/L), and cytotostatic is suspended in nutrient solution, brings very large challenge to gathering; Therefore, generally all first carry out pre-treatment to algae liquid for gathering of micro-algae both at home and abroad, micro-concentration of algae can be concentrated to 1% by flocculation and precipitation technology, and the collection that technique realizes micro-algae such as then carry out filtering or centrifugal again, its yield is 80%.For scale operation, current flocculation, precipitation, filtration and centrifuging process efficiency is low, energy consumption is high, total cost is even worth higher than biofuel products itself.The separating technology optimizing algae is the key problem in technology reducing production cost, realize microdisk electrode industrialization.
Summary of the invention
The object of the invention is the many deficiencies overcoming above-mentioned prior art, and a kind of microorganism culturing film is provided, microorganism culturing film has three-dimensional porous structure, apposition growth is carried out for microorganism, and it is insoluble to have normal temperature (under culture temperature), the characteristic of rising temperature for dissolving, simple rising temperature for dissolving is only needed after cultivation completes, filter the Efficient Collection that just can realize microorganism, culture membrane after dissolving, after centrifuging, filtrate can be used as spinning solution and reclaims, and become microorganism culturing film by electrostatic spinning process secondary processing, realize the recycling of microorganism culturing film,
There is provided a kind of microbial culture system on the other hand, be composited by even liquid feed device and microorganism culturing film, microorganism culturing film is replaceable, be convenient to the collection of microorganism, the vesicular structure of even liquid feed device, by syphonic effect, realize protecting liquid and feed flow, for microorganism culturing film provides sufficient nutrient solution.
For realizing such scheme, technical scheme of the present invention is as follows:
First, microorganism culturing film provided by the invention, it is a kind of 0-45 DEG C and does not dissolve, and is warming up to the water-soluble polymer film of the vesicular structure that 45-150 DEG C dissolves.
The material of described water-soluble polymer film is carboxymethyl starch, starch acetates, polyacrylic acid, polymaleic anhydride, modified methylated cellulose class CMC, polyoxyethylene, polyvinyl alcohol, polyoxyethylene glycol, modified polyvinyl alcohol, carboxymethyl cellulose, polyacrylamide or polyvinylpyrrolidone.
Described water-soluble polymer film is the water-soluble polymer film with three-dimensional porous structure obtained by electrostatic spinning process, and its thickness is 1-50 μm.
The present invention also provides a kind of microorganism culturing recovery system with microorganism culturing film, and it is made up of microorganism culturing film and liquid feed device; Described microorganism culturing film is that a kind of 0-45 DEG C does not dissolve, and is warming up to the water-soluble polymer film of the vesicular structure that 45-150 DEG C dissolves; Described liquid feed device is made up of porous material substrate, the feed pipe being fixedly loaded on described porous material substrate upper end and the collector tube that is fixedly loaded on described porous material substrate lower end;
Described porous material substrate be porous material make there is syphonic effect to realize even feed flow and to protect the sheet material of liquid; The described feed pipe being fixedly installed in porous material substrate upper end is connected with the porous in described porous material substrate; The described collector tube being fixedly installed in porous material substrate lower end is connected with the porous in described porous material substrate; Described microorganism culturing film adheres on described porous material substrate surface.
Described porous material substrate is the polyurethane foam board of 30-99% percentage of open area.
The material of described water-soluble polymer film is carboxymethyl starch, starch acetates, polyacrylic acid, polymaleic anhydride, modified methylated cellulose class CMC, polyoxyethylene, polyvinyl alcohol, polyoxyethylene glycol, modified polyvinyl alcohol, carboxymethyl cellulose, polyacrylamide or polyvinylpyrrolidone.
Described water-soluble polymer film is the water-soluble polymer film with three-dimensional porous structure obtained by electrostatic spinning process, and its thickness is 1-50 μm.
The present invention has following effect:
Microorganism culturing film of the present invention has three-dimensional porous structure, has higher specific surface area, contributes to the adhesion growth of microorganism, improves product yield; Microorganism culturing film has that normal temperature (under culture temperature) is insoluble, the characteristic of rising temperature for dissolving; The microorganism culturing recovery system with microorganism culturing film of the present invention, only needs simple rising temperature for dissolving, filters the Efficient Collection that just can realize microorganism after its microorganism culturing Membrance cuiture completes; Microorganism culturing film after dissolving, after centrifuging, filtrate can be used as spinning solution, reclaims, and can be directly used in the forming process stating microorganism culturing film, realizes the repeatedly recycling of microorganism culturing film; And the replaceable of microorganism culturing film is easy to industrialization continuous seepage; Microorganism culturing membrane material is water-soluble polymer, and process solvent is water, and the course of processing is clean, pollution-free; Productive rate is high; Porous material liquid feed device realizes continuing by syphonic effect and uniform feed flow and guarantor's liquid, reduces energy consumption;
Accompanying drawing explanation
Fig. 1 is the structural representation of microorganism culturing film of the present invention and microorganism culturing recovery system;
Wherein: microorganism culturing film 1 porous material substrate 2 feed pipe 3 collector tube 4
Embodiment:
Further the present invention is set forth below in conjunction with drawings and the specific embodiments, should be understood that and quote embodiment only for illustration of the present invention, and be not used in and limit the scope of the invention.
Embodiment 1:
1) preparation of liquid feed device:
Water, amines catalyst NiaxA-230, organotin catalysts NiaxD-19 and organic foam stabilizer NiaxL-580 are joined successively and be loaded with in the container of polyether glycol, high-speed mixing stirs 45 seconds; Add isocyanic ester TDI80 again, continue stirring and be no less than 15 seconds, then pour in mould rapidly, after its free foaming, then 120 DEG C of slakings in an oven 24 hours will be put, and finally take out, make 100mm × 200mm × 10mm porous material substrate 2;
The weight of described polyether glycol, water, amines catalyst NiaxA-230, organotin catalysts NiaxD-19, organic foam stabilizer NiaxL-580 and isocyanic ester TDI80 is 100:3.8:0.24:0.22:0.8:60.30;
2) preparation of microorganism culturing film 1:
By soluble in water for PVAC polyvinylalcohol-1799, configuration solid content is the spinning solution of 8wt%, makes substrate with above-mentioned porous material substrate 2, and (spinning voltage is 12-30KV, is preferably 18KV to carry out spinning by electrostatic spinning process; Spinning temperature is 10-40 DEG C, is preferably 25 DEG C; Relative humidity is 15-70%, is preferably 40%; Spinning jet speed is 0.1-0.8ml/min, is preferably 0.2ml/min; Spinning top and dash receiver distance are 200-600mm, are preferably 320mm); PVAC polyvinylalcohol-1799 film (the microorganism culturing film 1) thickness made through electrostatic spinning process is 1-50 μm, is preferably 20 μm; Upset porous material substrate 2, carries out spinning by electrostatic spinning process at porous material substrate 2 opposite side, the three-layer composite structure part of obtained microorganism culturing film 1/ porous material substrate 2/ microorganism culturing film 1;
3) feed pipe 3 and collector tube 4 is connected respectively at the two ends up and down of three-layer composite structure part, just obtained microbial cultivation device; The micro-algae Palmellococcusminiatus of high lipid content is inoculated on microorganism culturing film;
4) according to table 1 recipe configuration nutritive medium, under then the culture apparatus having inoculated micro-algae Palmellococcusminiatus being placed in culture environment, and carry nutritive medium by feed pipe 3, unnecessary nutritive medium collected by collector tube 4.
Table 1 micro-algae Palmellococcusminiatus nutrient solution prescription
4) after having cultivated, take microorganism culturing film off, and put into water, be heated to 65 DEG C, stirring and dissolving; Filtration obtains micro-algae, analyzes micro-algae rate of recovery is 95-98%.
Embodiment 2:
1) preparation of liquid feed device:
Water, amines catalyst NiaxA-230, organotin catalysts NiaxD-19 and organic foam stabilizer NiaxL-580 are joined successively and be loaded with in the container of polyether glycol, high-speed mixing stirs 45 seconds; Add isocyanic ester TDI80 again, continue stirring and be no less than 15 seconds, then pour into rapidly in molding jig, after its free foaming, then 120 DEG C of slakings in an oven 24 hours will be put, and finally take out, make 100mm × 200mm × 10mm porous material substrate 2;
The weight of described polyether glycol, water, amines catalyst NiaxA-230, organotin catalysts NiaxD-19, organic foam stabilizer NiaxL-580 and isocyanic ester TDI80 is 100:3.8:0.24:0.22:0.8:60.30;
2) preparation of microorganism culturing film 1:
PVAC polyvinylalcohol fiber is prepared polyvinyl alcohol spunlace non-woven cloth by water jet process, and preferred thickness is 25-30 μm.Polyvinyl alcohol spunlace non-woven cloth is cut into 100mm × 200mm size, obtained microorganism culturing film.
The Fibre diameter of described polyvinyl alcohol fiber is 8-12 μm, length is 4-12mm.
The three-layer composite structure part of obtained microorganism culturing film 1/ porous material substrate 2/ microorganism culturing film 1;
3) preparation of microorganism culturing recovery system:
Mentioned microorganism culture membrane 1 is sticked to feed flow substrate both sides and obtain microorganism culturing film 1/ porous material substrate 2/ microorganism culturing film 1 three-layer composite structure part.Feed pipe 3 and collector tube 4 is connected respectively, just obtained microbial cultivation device at the two ends up and down of three-layer composite structure part; The micro-algae Palmellococcusminiatus of high lipid content is inoculated on microorganism culturing film;
4) according to table 2 recipe configuration nutritive medium, under then the culture apparatus having inoculated micro-algae Palmellococcusminiatus being placed in culture environment, and carry nutritive medium by feed pipe 3, unnecessary nutritive medium collected by collector tube 4.
Table 2 micro-algae Palmellococcusminiatus nutrient solution prescription
4) after having cultivated, take microorganism culturing film off, and put into water, be heated to 65 DEG C, stirring and dissolving; Filtration obtains micro-algae, analyzes micro-algae rate of recovery is 95-98%.
Above-mentionedly only list 2 embodiments.In fact, the material of water-soluble polymer film of the present invention is carboxymethyl starch, starch acetates, polyacrylic acid, polymaleic anhydride, modified methylated cellulose class CMC, polyoxyethylene, polyvinyl alcohol, polyoxyethylene glycol, modified polyvinyl alcohol, carboxymethyl cellulose, polyacrylamide or polyvinylpyrrolidone, does not do exhaustive repeating at this.
Claims (7)
1. a microorganism culturing film, it is a kind of 0-45 DEG C and does not dissolve, and is warming up to the water-soluble polymer film of the vesicular structure that 45-150 DEG C dissolves.
2. microorganism culturing film as claimed in claim 1, it is characterized in that, the material of described water-soluble polymer film is carboxymethyl starch, starch acetates, polyacrylic acid, polymaleic anhydride, modified methylated cellulose class CMC, polyoxyethylene, polyvinyl alcohol, polyoxyethylene glycol, modified polyvinyl alcohol, carboxymethyl cellulose, polyacrylamide or polyvinylpyrrolidone.
3. microorganism culturing film as claimed in claim 1, it is characterized in that, described water-soluble polymer film is the water-soluble polymer film with three-dimensional porous structure obtained by electrostatic spinning process, and its thickness is 1-50 μm.
4. have a microorganism culturing recovery system for microorganism culturing film, it is made up of microorganism culturing film and liquid feed device; Described microorganism culturing film is that a kind of 0-45 DEG C does not dissolve, and is warming up to the water-soluble polymer film of the vesicular structure that 45-150 DEG C dissolves; Described liquid feed device is made up of porous material substrate, the feed pipe being fixedly loaded on described porous material substrate upper end and the collector tube that is fixedly loaded on described porous material substrate lower end;
Described porous material substrate be porous material make there is syphonic effect to realize even feed flow and to protect the sheet material of liquid; The described feed pipe being fixedly installed in porous material substrate upper end is connected with the porous in described porous material substrate; The described collector tube being fixedly installed in porous material substrate lower end is connected with the porous in described porous material substrate; Described microorganism culturing film adheres on described porous material substrate surface.
5. have the microorganism culturing recovery system of microorganism culturing film as claimed in claim 4, it is characterized in that, described porous material substrate is the polyurethane foam board of 30-99% percentage of open area.
6. there is the microorganism culturing recovery system of microorganism culturing film as claimed in claim 4, it is characterized in that, the material of described water-soluble polymer film is carboxymethyl starch, starch acetates, polyacrylic acid, polymaleic anhydride, modified methylated cellulose class CMC, polyoxyethylene, polyvinyl alcohol, polyoxyethylene glycol, modified polyvinyl alcohol, carboxymethyl cellulose, polyacrylamide or polyvinylpyrrolidone.
7. have the microorganism culturing recovery system of microorganism culturing film as claimed in claim 4, it is characterized in that, described water-soluble polymer film is the water-soluble polymer film with three-dimensional porous structure obtained by electrostatic spinning process, and its thickness is 1-50 μm.
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