CN107384801A - Closing biological membrane type cultural method for industrialized production microalgae - Google Patents

Closing biological membrane type cultural method for industrialized production microalgae Download PDF

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CN107384801A
CN107384801A CN201710719225.6A CN201710719225A CN107384801A CN 107384801 A CN107384801 A CN 107384801A CN 201710719225 A CN201710719225 A CN 201710719225A CN 107384801 A CN107384801 A CN 107384801A
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microalgae
cell
industrialized production
membrane
microalgae cell
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李彤
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李彤
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12Unicellular algae; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/02Membranes; Filters

Abstract

The present invention relates to microalgae culture method technical field, is a kind of closing biological membrane type cultural method for industrialized production microalgae, carries out as follows:Microalgae cell is seeded on microalgae cell inoculating surfaces as supply range using a surface of microporous membrane as microalgae cell inoculating surfaces, another surface of the microporous membrane relative with microalgae cell inoculating surfaces.Closing biological membrane type cultural method of the present invention for industrialized production microalgae, microalgae biomass is separated with nutrient solution by microporous membrane, thoroughly solves the problems, such as cell detachment and biofilm detachment, pass through the buffer action of microporous membrane, it can prevent the pollutants such as the miscellaneous bacteria in nutrient solution from reaching in microalgae biomass, again by the covering buffer action of transparent membrane, so as to avoid pollution of the pollutants such as extraneous miscellaneous bacteria to microalgae biomass, so as to improve the purity of microalgae biomass(Purity reaches more than 99%)And quality.

Description

Closing biological membrane type cultural method for industrialized production microalgae
Technical field
The present invention relates to microalgae culture method technical field, is a kind of closing biological membrane type for industrialized production microalgae Cultural method.
Background technology
Microalgae refers to eucaryon or protokaryon and can carry out photosynthetic microorganism.The protein content of some microalgaes is higher, Food, aquatic feed or animal and fowl fodder can be used as(Such as spirulina);Some microalgaes can largely synthesize secondary under given conditions Raw metabolin, such as grease, pigment, polysaccharide(The astaxanthin as caused by haematococcus pluvialis), these products can be used in function The fields such as food, food additives, pharmacy, bioenergy, there is considerable economic value.In addition, biomass extraction microalgae oil Fat and convert production biodiesel be considered as one of most important approach of carbon fixation and emission reduction.At present, in the world, microalgae is given birth to Thing technology has formd a complete huge industrial chain, and the industrialized production of wherein microalgae is most important one Link.
The current industrialization microalgae culture system that production has been put into uses liquid submersion culture, in immersion culture In system, microalgae cell is scattered in nutrient solution(Biomass content is less than 1%).Immersion culture systems include open culture Pond and Closed photobioreactor(Photobioreactor, PBR)Two kinds of forms.The advantages of open culture pond is mainly Build and the cost of operation is relatively low.But because the open area/volume in open pond is smaller, the microalgae only close to liquid-covered Cell can receive more sufficient illumination;Simultaneously as the opening of system and quickly mixing, after miscellaneous bacteria enters system Rapid diffusion, causes system to be highly prone to pollute.PBR is typically to use light transmissive material(Such as glass, lucite, plastic sheeting Deng)Manufactured enclosed construction, because optical path is short, cultivating system illuminating area/volume is bigger, so cell illumination is more abundant; Meanwhile the closure of system reduces the miscellaneous bacteria infection rate of system.But PBR generally involves great expense, operating cost is high, it is tired to safeguard It is difficult.The characteristics of growing, carbon dioxide are submerged between microalgae cell(CO2)Microalgae cell need to be got to by nutrient solution, therefore The benefit carbon efficiencies of immersion culture systems are relatively low.Meanwhile the nutrient solution of large volume result in the high operation of immersion culture systems Cost:Mass energy consumption in the links such as the agitation cycle of nutrient solution, temperature control, ventilation;Nutrient solution in itself, including inorganic nutrients With a large amount of consumption of water.Meanwhile need to expend in view of the characteristics of immersion culture systems biomass content is low, during harvest substantial amounts of Energy is dehydrated to the suspension of harvest(Up to the 30% of overall running cost).
In recent years, by the improvement to system, the problem of immersion microalgae culture system efficiency is low, has obtained certain improvement. Such as the operation of shallow pond, the reinforcing of split flow plate washer in open pond mix, benefit carbon is strengthened(The China of Application No. 200510126465.2 is specially Sharp document discloses the carbon compensator and its application method for large-scale culturing micro-algae;In Application No. 200610018771 State's patent document discloses the device of supplementing CO 2 to micro-algae culture pond);Helix tube type, duct type, gas-lifting type etc. are various close The structure of enclosed bioreactor is improved, and open raceway pond combines with coupling for Closed photobioreactor(Shen Number it please disclose a kind of device and cultural method for microalgae mass culture for 2010101363004 Chinese patent literature) Deng.But these improve the breakthrough not in system nature.In brief, due to the limitation of immersion culture systems, these pins Further optimization to immersion culture systems does not have the light utilization ratio for the system that is significantly increased, improves unit area production Measure or reduce cost.Therefore, microdisk electrode technology is inherently reformed, it is thin particularly to improve microalgae contained by unit volume nutrient solution The amount of born of the same parents(Cell concentration), to reduce construction, operating cost, material consumption energy consumption is reduced, reduces and takes up an area, and then improves space utilization Rate, it is the core demand of current microalgae industry.Meanwhile in order to meet to produce higher quality microalgae product(Such as medicine)Demand, Quality and purity requirement to microalgae biomass is also increasingly stricter, therefore it is micro- to develop the more inexpensive production high-quality of higher efficiency The culture systems of algal biomass, become one of top priority of current microdisk electrode technological development.
Theoretically, most of microalgae it is not absolutely required to be grown in the environment of suspension, for example, using microalgae as The photosynthetic organism film of main constituents is just distributed widely in nature.The biomass content of biomembrane(More than 30%)It is much high In the suspension of immersion culture systems.At present, it is in the presence of the production that microalgae biomass is carried out using photosynthetic organism film System(Such as:Setlik I, Sust V, Malek I. Dual purpose open circulation unit for large scale culture of algea in temperate zones. I. Basic design considerations and scheme of pilotplant. Algological Studies 1970;1: 111- 164).Compared to the suspension of traditional immersion culture systems, the biology when main advantage of biological membrane type system is to harvest The high biomass concentration of film, based on this, the cost that biomass is dehydrated after harvest can substantially reduce, and be produced into so as to effectively reduce This.Existing biofilms formula system mainly photosynthetic organism film is immersed in one layer of shallower nutrient solution is cultivated, so System set, compared to traditional immersion culture systems, although effectively increasing the concentration of biomass, in other respects Have no significant improvement.For example, the light utilization efficiency of system is relatively low, system vulnerable to pollution, system operation cost are high and/or system is mended The problem of carbon efficiencies are low is not improved fundamentally.Meanwhile also generated using the biofilm system of this immersion Other problemses:Due to the flowing of nutrient solution(Or biomembrane motion)Caused stress, training is entered after cell detachment, biofilm detachment Nutrient solution, cause the further decline of light utilization efficiency and the reduction of yield of biomass.Document also reports a kind of non-immersion life Thing membranous system(Application No. 201010250866.X Chinese patent literature disclose it is a kind of for the production of microalgae industry metaplasia half Process for solid culture), in this culture systems, microalgae is seeded on the material with water retention, in this way, microalgae cell is not Needing to be submerged also be kept and moisten and grow, system as described above reduces the Culture liquid measure needed for system operation, Operating cost is reduced while improving biomass concentration.But the system described by the document does not solve fundamentally Cell and/or biomembrane, which depart from, enters nutrient solution.One kind of Application No. 201210051158.2 is produced for microalgae industry metaplasia Semisolid adherent culture device and Application No. 201210051552.6 a kind of board plug type microalgae semisolid adhere-wall culture dress The Chinese patent literature put, in the process of running, cell can be grown its device in water material internal, be so as to block System, increase system operation maintenance cost, and after miscellaneous bacteria enters nutrient solution, miscellaneous bacteria can follow nutrient solution to reach microalgae inoculation Face, so that microalgae biomass easily by living contaminants, reduces the quality and purity of microalgae biomass, in above-mentioned patent document After system maximization, it is difficult to carry out closed sterile soft culture.
The content of the invention
The invention provides a kind of closing biological membrane type cultural method for industrialized production microalgae, overcome above-mentioned existing There is the deficiency of technology, it can effectively solve existing microalgae culture method and microalgae biomass be present easily by contaminants such as miscellaneous bacterias The problem of.
The technical scheme is that realized by following measures:A kind of closing for industrialized production microalgae is given birth to Thing membrane type cultural method, is carried out as follows:It is thin with microalgae using a surface of microporous membrane as microalgae cell inoculating surfaces Microalgae cell is seeded on microalgae cell inoculating surfaces as supply range by another surface of the relative microporous membrane of born of the same parents' inoculating surfaces, The micropore that microalgae cell cannot pass through microporous membrane reaches supply range, and after inoculation, microalgae cell inoculating surfaces are used into transparent membrane Covering, gas phase and nutrient solution containing carbon dioxide are supplied in microalgae cell from supply range, and carbon dioxide and culture liquid energy are worn The micropore arrival inoculating surfaces for crossing microporous membrane provide nutrient for the culture of microalgae cell, and illumination is supplied to inoculation by transparent membrane Microalgae cell on microalgae cell inoculating surfaces, in the incubation of microalgae cell, when microalgae cell and/or microalgae secondary generation Thank product concentration reach desired value after, microalgae biomass is harvested.
Here is the further optimization and/or improvements to foregoing invention technical scheme:
Above-mentioned microporous membrane is the filtering material that aperture is less than microalgae cell, and filtering material is filter paper or filter membrane or fabric; And/or transparent membrane is transparent nontoxic thin-film material, transparent membrane is pellicle or non-porous transparent membrane, the hole of pellicle The aperture of footpath≤microporous membrane micropore.
The surface of above-mentioned microporous membrane and transparent membrane is plane or curved surface.
The above-mentioned gas phase containing carbon dioxide and nutrient solution are supplied in microalgae cell from the supply range of microporous membrane respectively; Or the nutrient solution containing carbon dioxide is supplied in microalgae cell from the supply range of microporous membrane.
Above-mentioned microalgae is haematococcus pluvialis grid algae or chlorella or micro- plan ball algae or phycobiont;Microalgae secondary metabolite refers to The secondary metabolite produced by microalgae cell, secondary metabolite be in astaxanthin, carotenoid and dinoflagellate element it is a kind of with On.
The accumulation of above-mentioned microalgae biomass and microalgae secondary metabolite is individually accumulation or sequentially accumulates or carries out simultaneously Accumulation.
The light source of above-mentioned illumination is natural light and/or artificial light source.
Above-mentioned inoculation refers to the method that microalgae cell can be placed to microalgae cell inoculating surfaces, inoculation method using filtering or Smear or spray or inject;And/or harvest the method for referring to microalgae cell can be gathered from microalgae cell inoculating surfaces, harvesting method Using spatula and/or flushing.
Above-mentioned nutrient solution refers to containing microalgaeCellThe fluid nutrient medium of whole nutritional ingredients needed for growth, nutritional ingredient Including nitrogen, phosphorus, carbon, trace element and regulatory factor, regulatory factor includes inducible factor and/or stress factors.
Above-mentioned gas are mutually air or other gases.
Closing biological membrane type cultural method of the present invention for industrialized production microalgae, by microalgae biomass and battalion Nutrient solution is separated by microporous membrane, thoroughly solves the problems, such as cell detachment and biofilm detachment, passes through the isolation of microporous membrane It effect, can prevent the pollutants such as the miscellaneous bacteria in nutrient solution from reaching in microalgae biomass, then be isolated by the covering of transparent membrane Effect, so as to avoid pollution of the pollutants such as extraneous miscellaneous bacteria to microalgae biomass, so as to improve the purity of microalgae biomass (Purity reaches more than 99%)And quality;Meanwhile, it is capable to keep microalgae cell to be centrally located on microalgae cell inoculating surfaces, Jin Erneng Enough biomass dehydration costs reduced when harvesting, it is possible to increase the yield of microalgae biomass, improve microalgae biomass and Qi Dai Thank to the production efficiency of product;Due to will not blocking microporous film micropore, so eliminate solve blockage problem maintenance cost;This The described closing biological membrane type cultural method for industrialized production microalgae of invention not only remains existing photosynthetic organism membrane type The advantages of culture;And compared to immersion culture, use the closing biology of the present invention for industrialized production microalgae After membrane type cultural method, the usage amount of nutrient solution can be reduced, obtaining 1Kg dry biomass needs nutrient solution to be less than 10L, and it is cultivated Liquid usage amount is 0.05 to 0.07 times of traditional immersion culture.
Brief description of the drawings
Accompanying drawing 1 is the schematic diagram of microporous membrane in a state of use in the embodiment of the present invention 1.
Coding in accompanying drawing is respectively:1 is microporous membrane, and 2 be microalgae cell inoculating surfaces, and 3 be supply range, and 4 be thin transparent Film, 5 be microalgae cell, and 6 be the biomass that microalgae is formed(The photosynthetic organism film that microalgae is formed), 7 be microalgae biomass(Photosynthetic life Thing film)It is microalgae biomass with the mass exchange of gas phase, 8(Photosynthetic organism film)It is illumination with the mass exchange of liquid phase, 9.
Embodiment
The present invention is not limited by following embodiments, can technique according to the invention scheme and actual conditions it is specific to determine Embodiment.Various chemical reagent and chemical article are previously mentioned in the present invention unless otherwise specified, are public in the prior art Know public chemical reagent and chemical article;Percentage in the present invention is mass percent as not having specified otherwise;This hair Normal temperature, room temperature in bright refer generally to 15 DEG C to 25 DEG C of temperature, are commonly defined as 25 DEG C.
With reference to embodiment, the invention will be further described:
Embodiment 1:As shown in Figure 1, the closing biological membrane type cultural method for being used for industrialized production microalgae, as follows Carry out:Using a surface of microporous membrane 1 as microalgae cell inoculating surfaces 2, the microporous membrane relative with microalgae cell inoculating surfaces 2 Microalgae cell 5 is seeded on microalgae cell inoculating surfaces 2, microalgae cell 5 cannot pass through micro- by 1 another surface as supply range 3 The micropore of hole film 1 reaches supply range 3, after inoculation, microalgae cell inoculating surfaces 2 is covered using transparent membrane 4, contain titanium dioxide The gas phase and nutrient solution of carbon are supplied in microalgae cell 5, carbon dioxide and culture liquid energy through microporous membrane 1 from supply range 3 Micropore reaches inoculating surfaces and provides nutrient for the culture of microalgae cell 5, and illumination is supplied to by transparent membrane 4 and is inoculated in microalgae cell Microalgae cell 5 on inoculating surfaces 2, in the incubation of microalgae cell 5, when microalgae cell 5 and/or microalgae secondary metabolite Concentration reach desired value after, microalgae biomass is harvested.
Here microalgae biomass refers to whole microalgae cell group, i.e., the biomass 6 that microalgae is formed.
The closing biological membrane type cultural method for industrialized production microalgae described in the present embodiment, by microalgae biomass with Nutrient solution is separated by microporous membrane 1, thoroughly solves the problems, such as cell detachment and biofilm detachment, also, microporous membrane 1 is not Inoculation medium only as microalgae cell 5, and carbon dioxide and nutrient solution can be from supply ranges 3 to inoculating surfaces(Microalgae cell Inoculating surfaces 2)On microalgae cell 5 provide nutrient.By the buffer action of microporous membrane 1, the miscellaneous bacteria in nutrient solution can be prevented Reached Deng pollutant in microalgae biomass, then the covering buffer action by transparent membrane 4, so as to avoid the pollutants such as miscellaneous bacteria Pollution to microalgae biomass, so as to improve the purity of microalgae biomass(Purity reaches more than 99%)And quality;
Meanwhile, it is capable to keep microalgae cell 5 to be centrally located on microalgae cell inoculating surfaces 2, and then biology when can reduce harvest Matter dehydration cost, it is possible to increase the yield of microalgae biomass(Total biomass weight when microalgae biomass dry weight has reached harvest More than 22%), improve microalgae biomass(Dry biomass yield is daily up to every square metre of floor space of 1kg)With its metabolism production The production efficiency of thing;Due to will not the blocking microporous micropore of film 1, so eliminate solve blockage problem maintenance cost.
The closing biological membrane type cultural method for industrialized production microalgae described in the present embodiment remains existing photosynthetic The advantages of biological membrane type culture;Meanwhile compared to immersion culture, it is used for industrialized production microalgae using described in the present embodiment Closing biological membrane type cultural method after, the usage amount of nutrient solution can be reduced, obtaining 1Kg dry biomass needs nutrient solution to be less than 10L, its nutrient solution usage amount are 0.05 to 0.07 times of traditional immersion culture.
Embodiment 2:As the optimization of above-described embodiment, microporous membrane 1 is the filtering material that aperture is less than microalgae cell 5, Filtering material is filter paper or filter membrane or fabric;And/or transparent membrane 4 is transparent nontoxic thin-film material, transparent membrane 4 For pellicle or non-porous transparent membrane, the aperture of the micropore of aperture≤microporous membrane 1 of pellicle.
Filtering material can use existing cheap material, so as to reduce production cost.
Embodiment 3:As the optimization of above-described embodiment, the surface of microporous membrane 1 and transparent membrane 4 is plane or curved surface.
Embodiment 4:As the optimization of above-described embodiment, gas phase and nutrient solution containing carbon dioxide are thin from micropore respectively The supply range 3 of film 1 is supplied in microalgae cell 5;Or the nutrient solution containing carbon dioxide is supplied from the supply range 3 of microporous membrane 1 Award microalgae cell 5.
Embodiment 5:As the optimization of above-described embodiment, microalgae be haematococcus pluvialis grid algae or chlorella or micro- plan ball algae or Phycobiont;Microalgae secondary metabolite refers to the secondary metabolite produced by microalgae cell, and secondary metabolite is astaxanthin, class One or more of carrotene and dinoflagellate element.
Embodiment 6:As the optimization of above-described embodiment, the accumulation of microalgae biomass and microalgae secondary metabolite is independent Accumulation is sequentially accumulated or accumulated simultaneously.
Embodiment 7:As the optimization of above-described embodiment, the light source of illumination is natural light and/or artificial light source.
Embodiment 8:As the optimization of above-described embodiment, inoculation refers to microalgae cell 5 can be placed to microalgae cell inoculation The method in face 2, inoculation method is using filtering or smears or sprays or injects;And/or refer to can be from microalgae cell inoculating surfaces for harvest The method of 2 collection microalgae cells 5, harvesting method use spatula and/or flushing.
Embodiment 9:As the optimization of above-described embodiment, nutrient solution refers to whole nutrition needed for containing microalgae cell growth The fluid nutrient medium of composition, nutritional ingredient include nitrogen, phosphorus, carbon, trace element and regulatory factor, and regulatory factor includes inducible factor And/or stress factors.
Inducible factor, stress factors are the known technology of existing microdisk electrode.
Embodiment 10:As the optimization of above-described embodiment, gas phase is air or other gases.
Embodiment 11:This is used for the closing biological membrane type cultural method of industrialized production microalgae, carries out as follows:Will Long 0.5m, wide 0.1m, the fiberglass gridding that thickness is 1mm(Square mesh, length and width are 1cm)It is vertically placed on a diameter of 0.12m, highly for 0.55m transparent organic glass pipe in.Using peristaltic pump from the top of fiberglass gridding, to glass fibre BBM nutrient solutions are added in grid, culture flow quantity is 3mLmin-1, nutrient solution cumulative volume be 1L and be stored in a volume be In 1.2L container.In the lower section of lucite tube, the nutrient solution flowed out with collection funnel is simultaneously flowed back in nutrient solution holding bottle. Pure grid frustule is seeded in the surface of nylon leaching film using the mode of filtering in an aseptic environment(Be inoculated with zone boundary with Nylon film frontier distance 0.05m), inoculation biological quality is 5g m-2.It is using long 0.5m, wide 0.1m, thickness after inoculation The one side that nylon micro porous film is inoculated with by 0.1mm FED transparent membranes 4 covers, and by all round closure.Then it is nylon micro porous is thin Film 1 is placed into lucite tube as described above, and the nonvaccinated one side of nylon micro porous film is attached into fiberglass gridding Surface.In incubation, illumination is provided to the nylon leaching film surface of inoculation using sodium lamp, light intensity is 1000 μm of olm-2s-1, And use 16:8 illumination --- dark cycle(Close to natural light), environment temperature is 26 during cultureoC.Pass through in incubation Air inlet provides the compressed air that carbon dioxide content is 3%, flow 1Lmin into lucite tube-1
As a result show, the yield of grid algal biomass has been up to 26gm-2d-1Nylon leaching film, convert and take up an area face for unit It is 1140gm after product yield of biomass-2d-1, than duty runway system(Use natural light)Have the maximum output of record at present (360gm-2d-1)It is high 3.2 times.Biomass dry weight after directly harvesting is the 21% of gross weight, is far longer than immersion system(It is small In 1%).
By the culture of 20 days, biomass was not by any is shown to the micro- sem observation of biomass after harvest simultaneously The pollution of miscellaneous bacteria.
In summary, the closing biological membrane type cultural method of the present invention for industrialized production microalgae, by microalgae Biomass is separated with nutrient solution by microporous membrane, thoroughly solves the problems, such as cell detachment and biofilm detachment, passes through micropore It the buffer action of film, can prevent the pollutants such as the miscellaneous bacteria in nutrient solution from reaching in microalgae biomass, then pass through transparent membrane Covering buffer action, so as to avoid pollution of the pollutants such as extraneous miscellaneous bacteria to microalgae biomass, so as to improve microalgae life The purity of material(Purity reaches more than 99%)And quality;Meanwhile, it is capable to microalgae cell is kept to be centrally located at microalgae cell inoculating surfaces On, and then biomass dehydration cost when can reduce harvest, it is possible to increase the yield of microalgae biomass, improve microalgae biology The production efficiency of matter and its metabolite;Due to will not blocking microporous film micropore, so eliminate solve blockage problem dimension Shield expense;Closing biological membrane type cultural method of the present invention for industrialized production microalgae not only remains existing photosynthetic The advantages of biological membrane type culture;And compared to immersion culture, using of the present invention for industrialized production microalgae After closing biological membrane type cultural method, the usage amount of nutrient solution can be reduced, obtaining 1Kg dry biomass needs nutrient solution to be less than 10L, its nutrient solution usage amount are 0.05 to 0.07 times of traditional immersion culture.
Above technical characteristic constitutes embodiments of the invention, and it has stronger adaptability and implementation result, can basis The non-essential technical characteristic of increase and decrease is actually needed, to meet the needs of different situations.

Claims (10)

1. a kind of closing biological membrane type cultural method for industrialized production microalgae, it is characterised in that carry out as follows: Using a surface of microporous membrane as microalgae cell inoculating surfaces, another table of the microporous membrane relative with microalgae cell inoculating surfaces Microalgae cell is seeded on microalgae cell inoculating surfaces, microalgae cell cannot pass through the micropore of microporous membrane by face as supply range Supply range is reached, after inoculation, microalgae cell inoculating surfaces are covered using transparent membrane, gas phase and culture containing carbon dioxide It is microalgae that liquid is supplied in microalgae cell, carbon dioxide and culture liquid energy to reach inoculating surfaces through the micropore of microporous membrane from supply range The culture of cell provides nutrient, and illumination is supplied to the microalgae cell being inoculated on microalgae cell inoculating surfaces by transparent membrane, In the incubation of microalgae cell, after the concentration of microalgae cell and/or microalgae secondary metabolite reaches desired value, to microalgae Biomass is harvested.
2. the closing biological membrane type cultural method according to claim 1 for industrialized production microalgae, it is characterised in that Microporous membrane is the filtering material that aperture is less than microalgae cell, and filtering material is filter paper or filter membrane or fabric;And/or thoroughly Bright film is transparent nontoxic thin-film material, and transparent membrane is pellicle or non-porous transparent membrane, aperture≤micropore of pellicle The aperture of film micropore.
3. the closing biological membrane type cultural method according to claim 1 or 2 for industrialized production microalgae, its feature exists In the surface of microporous membrane and transparent membrane be plane or curved surface.
4. the closing biological membrane type cultural method for industrialized production microalgae according to claim 1 or 2 or 3, it is special Sign is that the gas phase containing carbon dioxide and nutrient solution are supplied in microalgae cell from the supply range of microporous membrane respectively;Or Nutrient solution containing carbon dioxide is supplied in microalgae cell from the supply range of microporous membrane.
5. the closing biological membrane type cultural method for industrialized production microalgae according to claim 1 or 2 or 3 or 4, its It is characterised by microalgae for haematococcus pluvialis grid algae or chlorella or micro- plan ball algae or phycobiont;Microalgae secondary metabolite refers to by micro- The secondary metabolite of frustule production, secondary metabolite are one or more of astaxanthin, carotenoid and dinoflagellate element.
6. the closing biological membrane type culture side for industrialized production microalgae according to claim 1 or 2 or 3 or 4 or 5 Method, it is characterised in that the accumulation of microalgae biomass and microalgae secondary metabolite is individually accumulation or sequentially accumulates or carries out simultaneously Accumulation.
7. the closing biological membrane type culture for industrialized production microalgae according to claim 1 or 2 or 3 or 4 or 5 or 6 Method, it is characterised in that the light source of illumination is natural light and/or artificial light source.
8. the closing biological membrane type for industrialized production microalgae according to claim 1 or 2 or 3 or 4 or 5 or 6 or 7 is trained The method of supporting, it is characterised in that inoculation refers to the method that microalgae cell can be placed to microalgae cell inoculating surfaces, and inoculation method uses Filtering is smeared or sprayed or injects;And/or harvest refers to receive from the method for microalgae cell inoculating surfaces collection microalgae cell Obtain method and use spatula and/or flushing.
9. the closing biomembrane for industrialized production microalgae according to claim 1 or 2 or 3 or 4 or 5 or 6 or 7 or 8 Formula cultural method, it is characterised in that nutrient solution refers to the Liquid Culture containing whole nutritional ingredients needed for microalgae cell growth Base, nutritional ingredient include nitrogen, phosphorus, carbon, trace element and regulatory factor, and regulatory factor includes inducible factor and/or stress factors.
10. the closing for industrialized production microalgae according to claim 1 or 2 or 3 or 4 or 5 or 6 or 7 or 8 or 9 is given birth to Thing membrane type cultural method, it is characterised in that gas phase is air or other gases.
CN201710719225.6A 2017-08-21 2017-08-21 Closing biological membrane type cultural method for industrialized production microalgae Pending CN107384801A (en)

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