CN101144058A - Micro-algae culture medium for astaxanthin - Google Patents
Micro-algae culture medium for astaxanthin Download PDFInfo
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- CN101144058A CN101144058A CNA2007100093970A CN200710009397A CN101144058A CN 101144058 A CN101144058 A CN 101144058A CN A2007100093970 A CNA2007100093970 A CN A2007100093970A CN 200710009397 A CN200710009397 A CN 200710009397A CN 101144058 A CN101144058 A CN 101144058A
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- astaxanthin
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Abstract
A microalgae cultivation medium of astaxanthin relates to an alga cultivation medium, in particular to the microalgae cultivation medium of the astaxanthin. The improved microalgae cultivation medium of the astaxanthin is provided and named as CBBM cultivation medium. The amount of NaAc, vitamin B <SUB> 12 </SUB> and EDTA-Fe <SUP> 3 </SUP> is added based on the compounding formula of the original non-iron BBM cultivation medium. The growth of the haematococcus pluvialis and the accumulation aspect of the astaxanthin are both obviously improved. Through the multi-factor orthogonal experiment, the obtained improved proposal of the optimum astaxanthin includes that NaAc, the vitamin B <SUB> 12 </SUB> and EDTA-Fe <SUP> 3 </SUP> are added on the basis of the non-iron BBM compounding formula, the total content accounts for about 58 percent of the gross weight of the cultivation medium, the growth index of cultivating the haematococcus pluvialis can reach to 0.5, the output of the astaxanthin is 2.58 mg/L (20.43 mg/g), and the spore percentage is 76.9 percent. The growth speed of the haematococcus pluvialis can be speeded up, and the growth cycle is shortened.
Description
Technical field
The present invention relates to a kind of ball algae culture medium, especially relate to a kind of micro-algae culture medium of astaxanthin.
Background technology
Bischoff and Bold has invented a kind of substratum of cultivating little algae in 1963, and is the BBM substratum with the two naming, promptly original BBM substratum.After quote in many ways, slight change has taken place, the prescription of the BBM substratum of widespread use at present is as shown in table 1.
The composition of the original BBM substratum of table 1
| Composition | Concentration (mgL -1) |
| NaNO 3 MgSO 4·7H 2O NaCl K 2HPO 4 KH 2PO 4 CaCl 2·2H 2O ZnSO 4·7H 2O MnCl 2·4H 2O MoO 3 CuSO 4·5H 2O CoNO 3·6H 2O H 3BO 3 EDTA KOH FeSO 4·7H 2O | 250 75 25 75 175 25 8.82 1.44 0.71 1.57 0.49 11.42 50 31 4.98 |
Studies show that when Haematocoocus Pluvialls was cultivated, its speed of growth was slow in original BBM substratum, spore ratio height, and chlorophyll, carotenoid and content astaxanthin are all lower.
Cao Pinghua etc. (Cao Pinghua, Li Xiaoxia, Yu Xueli, Tao Lin. the growth result of Haematocoocus Pluvialls in different substratum be [J] Henan agricultural sciences relatively, and 2004, (4)) from pH value, chlorophyll a content, A
674Value and 4 aspect systematic comparisons of carotenoid content the influences that Haematocoocus Pluvialls F712 is grown of 6 kinds of substratum.Found that in the BBM substratum, the rate of propagation of Haematocoocus Pluvialls is not remarkable, in the time of the 8th day, chlorophyll a content is 1.23mg/L only in the BBM substratum, is starkly lower than the chlorophyll a content (2.83mg/L) in the SM substratum; Be cultured to the 10th day, the content of total carotinoid is 0.55mg/L in the BBM substratum, significantly is lower than the content (1.39mg/L) of the total carotinoid in the SM substratum.
(Wuzhong is emerging for the emerging grade in Wuzhong, Zhuan Huiru. different substratum are to haematococcus pluvialis growing and astaxanthin cumulative influence [J]. Fujian Normal University's journal (natural science edition), 2002, (2)) with two kinds of substratum of BBM and HGZ the Haematocoocus Pluvialls of 3 different strains is cultivated, by growth velocity, dry weight, chlorophyll and astaxanthin semi-invariant being measured more different substratum and of growth and the astaxanthin cumulative influence of different culture condition to haematococcus pulvialis.The result shows, the nourishing and growing of the suitable relatively Haematocoocus Pluvialls of BBM substratum, but be unfavorable for the accumulation of astaxanthin.
Substratum is the most basic environment and the nutritional condition that Haematocoocus Pluvialls is cultivated, and its research is basic fundamental condition (the Fabregas J of Haematocoocus Pluvialls research and development with using; Dominguez A; Et al.Optimization of culture medium for thecontinuous cultivation of the microalga Haematococcus pluvialis.APPLIDE MICROBIOLOGYAND BIOTECHNOLOGY 53 (5): 530-535 MAY 2000).
In recent years, more abundant to major constituents research in the Haematocoocus Pluvialls substratum both at home and abroad, but to less about the research of minor component influence.
(Sun Yanni such as Sun Yanni, Yin Mingyan, Liu Jianguo. the semiochemicals of Haematocoocus Pluvialls [J]. ocean lakes and marhshes circular, 2001, (3)) report points out that haematococcus pluvialis growing speed may can improve its speed of growth by adding micro substance because some micro substance that wanes causes slowly.
The pertinent literature report, material such as VITAMIN and molysite to micro algae growth have good promoter action (1, Zhu Congju, Qi Yuzao, Guo Changbi. iron, nitrogen, phosphorus, vitamins B _ 1 and B_ (12) are to the growth effect [J] of ocean Prorocentrum. Oceanologia et Limnologia Sinica, 1994, (2).; 2, Zhou Rulun, Yang Zhen, Sun Aishu. the trophic component research [J] of Isochrysis galbana 8701 nutrient solutions. ocean lakes and marhshes circular, 1994, (1); 3, Zhu Mingyuan, Mu Xueyan, Li Ruixiang, Lv Ruihua. iron is to the influence [J] of Phaeodactylum tricornutum growth, photosynthesis and biochemical composition. ocean journal, 2000, (1); 4, Chen Cimei, Zheng Airong, week is kind to, Chen Yuzhou. the influence [J] of the growth of iron centering Skeletonemacostatum, pigmented degree and nitrogen assimilation ability. ocean journal, 1997, (3)).
Summary of the invention
The object of the present invention is to provide a kind of micro-algae culture medium of astaxanthin of improvement, and called after CBBM substratum.
Technical scheme of the present invention is to add an amount of NaAc, vitamins B on the prescription basis of the original BBM substratum of iron-free
12And EDTA-Fe
3+
Each composition of composition of the present invention and concentration thereof are as shown in table 2.
Table 2 composition of the present invention
| Composition | Concentration (mgL -1) |
| NaNO 3 MgSO 4·7H 2O NaCl K 2HPO 4 KH 2PO 4 CaCl 2 ZnSO 4·7H 2O MnCl 2·4H 2O MoO 3 CuSO 4·5H 2O CoNO 3·6H 2O 4H 3BO 3 EDTA KOH NaAc | 200～300 70～80 20～30 70～80 170～180 20～30 8～9 0.1～0.2 0.6～0.8 1～2 0.4～0.6 10～20 40～60 30～35 1000 |
| Vitamins B 12 | 0.05～0.06 |
| EDTA-Fe 3+ | 4～5 |
Compare with original BBM substratum, the present invention all is being significantly improved aspect the growth of Haematocoocus Pluvialls and the astaxanthin accumulation.Main Conclusions is as follows: the best medium evolutionary approach that obtains by the multiplefactor orthogonal experiment is to add NaAc, vitamins B on the BBM of iron-free prescription basis
12And EDTA-Fe
3+, its total content accounts for about 58% of substratum gross weight, and the growth index of cultivating Haematocoocus Pluvialls can reach 0.5, and astaxanthin yield is 22.58mg/L (20.43mg/g), and the spore ratio is 76.9%.Concrete every index relatively sees Table 3.The present invention can accelerate the speed of growth of Haematocoocus Pluvialls, shortens growth cycle.
The original BBM substratum of table 3 and the present invention compare the influence of haematococcus pluvialis growing and astaxanthin yield
| Substratum | Growth index | Survival rate (%) | Spore ratio (%) | Algae powder yield (gL -1) | Astaxanthin productive rate (mgL -1) | Content astaxanthin (mgg -1) |
| Original BBM substratum | 0.23 | 10.34 | 100％ | 0.76 | 10.05 | 13.22 |
| The present invention | 0.5 | 36.27 | 76.92 | 1.11 | 22.58 | 20.43 |
Embodiment
The concentration level that 3 sodium-acetates are set is respectively 0.5,1.0 and 2.0g/L, vitamins B
12Be provided with 0.005,0.05 and 3 concentration gradients such as 0.5ppm, iron is chosen EDTA-Fe
3+With two kinds of forms of ironic citrate, investigate sodium-acetate and vitamins B simultaneously
12Interaction, carry out L
18(2 * 3
4) orthogonal experiment, group is provided with as shown in table 4, and the BBM medium controls is set simultaneously.
The different substance concentration of table 4 are to haematococcus pluvialis growing and coerce the experimental design scheme that the stage influences
| Group | C NaAc(g/L) | C VB12(ppm) | The form of Fe |
| A1 A2 A3 A4 A5 A6 A7 A8 A9 A10 A11 A12 A13 A14 A15 A16 A17 A18 | 0.5 0.5 0.5 0.5 0.5 0.5 1.0 1.0 1.0 1.0 1.0 1.0 2.0 2.0 2.0 2.0 2.0 2.0 | 0.005 0.005 0.05 0.05 0.5 0.5 0.005 0.005 0.05 0.05 0.5 0.5 0.005 0.005 0.05 0.05 0.5 0.5 | EDTA-Fe 3+Ironic citrate EDTA-Fe 3+Ironic citrate EDTA-Fe 3+Ironic citrate EDTA-Fe 3+Ironic citrate EDTA-Fe 3+Ironic citrate EDTA-Fe 3+Ironic citrate EDTA-Fe 3+Ironic citrate EDTA-Fe 3+Ironic citrate EDTA-Fe 3+Ironic citrate |
Inoculative proportion is 1: 5, and inoculum density is 4 * 10
4Cellsml
-1, put and leave standstill cultivation in the illumination box.Temperature is controlled to be (23 ± 1.5) ℃, and light intensity is 1300～1500 lx, and photometry is shone continuously.Every experimental group is provided with two repetitions, and control group is made as 3 repetitions.Finish to go under the high light intensity condition in the suitable condition cultured continuously to exponential phase of growth, light intensity is 10000～12000 lx, inducing culture after 14 days the indexs such as frustule survival rate, spore ratio, algae powder productive rate and content astaxanthin to culture measure.
Claims (1)
1. the micro-algae culture medium of an astaxanthin is characterized in that each composition and the concentration thereof of its composition is as shown in the table.
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| CNA2007100093970A CN101144058A (en) | 2007-08-22 | 2007-08-22 | Micro-algae culture medium for astaxanthin |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102257955A (en) * | 2011-05-06 | 2011-11-30 | 中国科学院新疆生态与地理研究所 | Method for establishing syntrichia caninervis mitt. individual plant cloning system through asexual propagation |
| CN101803600B (en) * | 2009-02-13 | 2012-12-05 | 云南爱尔发生物技术有限公司 | Haematococcus pluvialis cell growth promoting agent and use method thereof |
| CN103589643A (en) * | 2013-11-27 | 2014-02-19 | 青岛旭能生物工程有限责任公司 | Haematococcus pluvialis culture medium |
| CN105316217A (en) * | 2015-04-17 | 2016-02-10 | 上海希明生物科技有限公司 | Artificial light source microalgae culture equipment |
| CN105916993A (en) * | 2014-04-17 | 2016-08-31 | 高丽大学校产学协力团 | Method for increasing production of astaxanthin in haematococcus pluvialis by mature spore inoculation and iron ion-mediated Harber-Weiss reaction at high temperature |
| CN107254411A (en) * | 2017-07-03 | 2017-10-17 | 山东鸣惠生物科技股份有限公司 | A kind of culture medium of haematococcus pluvialis |
| CN109182133A (en) * | 2018-10-25 | 2019-01-11 | 攀枝花学院 | Microalgae pure medium and the method for isolating and purifying microalgae |
| CN113549555A (en) * | 2021-09-08 | 2021-10-26 | 攀枝花市格萨拉生物技术有限责任公司 | Method for producing natural astaxanthin by culturing haematococcus pluvialis |
-
2007
- 2007-08-22 CN CNA2007100093970A patent/CN101144058A/en active Pending
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101803600B (en) * | 2009-02-13 | 2012-12-05 | 云南爱尔发生物技术有限公司 | Haematococcus pluvialis cell growth promoting agent and use method thereof |
| CN102257955A (en) * | 2011-05-06 | 2011-11-30 | 中国科学院新疆生态与地理研究所 | Method for establishing syntrichia caninervis mitt. individual plant cloning system through asexual propagation |
| CN102257955B (en) * | 2011-05-06 | 2012-12-26 | 中国科学院新疆生态与地理研究所 | Method for establishing syntrichia caninervis mitt. individual plant cloning system through asexual propagation |
| CN103589643A (en) * | 2013-11-27 | 2014-02-19 | 青岛旭能生物工程有限责任公司 | Haematococcus pluvialis culture medium |
| CN103589643B (en) * | 2013-11-27 | 2015-04-15 | 青岛旭能生物工程有限责任公司 | Haematococcus pluvialis culture medium |
| CN105916993A (en) * | 2014-04-17 | 2016-08-31 | 高丽大学校产学协力团 | Method for increasing production of astaxanthin in haematococcus pluvialis by mature spore inoculation and iron ion-mediated Harber-Weiss reaction at high temperature |
| CN105916993B (en) * | 2014-04-17 | 2019-09-27 | 高丽大学校产学协力团 | Method by improving the production of astaxanthin in haematococcus pluvialis in the Harber-Weiss reaction of high-temperature maturation spore inoculating and iron ion mediation |
| CN105316217A (en) * | 2015-04-17 | 2016-02-10 | 上海希明生物科技有限公司 | Artificial light source microalgae culture equipment |
| CN105316217B (en) * | 2015-04-17 | 2019-05-03 | 上海希明生物科技有限公司 | Artificial light source both culturing microalgae equipment |
| CN107254411A (en) * | 2017-07-03 | 2017-10-17 | 山东鸣惠生物科技股份有限公司 | A kind of culture medium of haematococcus pluvialis |
| CN109182133A (en) * | 2018-10-25 | 2019-01-11 | 攀枝花学院 | Microalgae pure medium and the method for isolating and purifying microalgae |
| CN113549555A (en) * | 2021-09-08 | 2021-10-26 | 攀枝花市格萨拉生物技术有限责任公司 | Method for producing natural astaxanthin by culturing haematococcus pluvialis |
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Open date: 20080319 |