CN103589643B - Haematococcus pluvialis culture medium - Google Patents
Haematococcus pluvialis culture medium Download PDFInfo
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Abstract
The invention provides a haematococcus pluvialis culture medium. The culture medium per liter contains 0.30-0.40g of NaNO3, 0.10-0.20g of KNO3, 0.07-0.18g of MgSO4.7H2O, 0.03-0.06g of Na2SO3, 0.10-0.30g of C6H5Na3O7, 0.02g of inorganic phosphorus, 0.005-0.02g of organic phosphorus, 0.03-0.09g of CaC12, 0.0002-0.0003g of EDTA-NaFe, 0.001-0.003g of FeSO4, 0.0015-0.0045g of ZnSO4, 0.00006-0.00018g of MnCl2, 0.00003-0.0001g of CoCl2, 0.0001-0.0003g of H3BO3, 0.00006-0.00018g of CuSO4, 0.00002-0.00006g of (NH4) Mo7O24, 0.002g of 3-IBA, 0.0003g of 6-BA and 0.000025-0.00005g of Vb12. The culture medium is used for photoautotrophically culturing haematococcus pluvialis, can significantly promote the growth of haematococcus pluvialis, enables the haematococcus pluvialis to rapidly enter the exponential growth phase so as to achieve higher growth density and keep the dominant position of the population of haematococcus pluvialis in a short time, and therefore is conducive to furthest reducing pollution probabilities of foreign bacteria, seaweeds and protozoa, shortening the growth cycle and increasing the successful probability of culture in the field enlarging culture process.
Description
Technical field
The present invention relates to biological technical field, particularly relate to a kind of Growth Medium For Haematococcus Pluvialis.
Background technology
Astaxanthin (Astaxanthin) is the fat soluble carotenoids with widespread use value and DEVELOPMENT PROSPECT, there is two major features: one is have extremely strong biological activity and physiological function, it is the one that nature known antioxidants is the strongest, its resistance of oxidation is 10 times of β-carotene, 100-550 times of vitamin-E, is described as " supper vitamin E "; Two is that color is scarlet, has very strong tinting strength.Research shows, astaxanthin has powerful scavenging free radicals and resistance of oxidation, have simultaneously significantly protect blood vessel, improve retina, prevent light injury, anti-ageing, anti-cancer and strengthen the effects such as body immunity.Therefore, astaxanthin is in healthcare products, medicine and makeup manufacture, and food and drink are processed, and the fields such as fishery products, poultry and livestock feed manufacturing all have wide application prospect.
The main production process of astaxanthin comprises synthetic and natural extract two class.Synthetic astaxanthin molecular structure and natural astaxanthin there are differences, and biological activity, far away from natural astaxanthin, biologic applications is only permitted for animal painted.The astaxanthin of natural extract obtains mainly through cultivating Haematocoocus Pluvialls, the fields such as food, health care and daily use chemicals industry can be widely used in, in new resource food catalogue (No. 17th, Ministry of Health bulletin) was listed Haematocoocus Pluvialls in 2010 by China, for the development in natural astaxanthin market at home lays the foundation.
At present, astaxanthin yield far can not meet market demand, causes international market price expensive, and only the raw material of synthetics is up to 2500 U.S. dollars/kilogram (patent No.: 200910300402.2), the product price of natural astaxanthin is then higher.Haematocoocus Pluvialls is a kind of unicell green alga, can accumulate astaxanthin in a large number under given conditions, reaches 1%-3% of frond dry weight, is the best Biological resources of potential production of astaxanthin.The haematococcus pluvialis cell growth cycle is complicated, and in current engineering culturing process, lower cell proliferation growth velocity becomes the main limiting factor limiting this algae development of resources.How effectively to improve frustule proliferative speed, be one of core of haematococcus pulvialis bio-resource exploitation to obtaining larger Biomass yield within the unit time.
In current haematococcus pulvialis substratum research, mainly utilize inorganic phosphorus as the substratum phosphorus source needed for cell construction, such as, patent: potassium primary phosphate (sodium) (patent of invention: application number 201310038104.7), dipotassium hydrogen phosphate (sodium) (patent of invention: application number 02138827.X); Metaphosphoric acid or ortho-phosphoric acid (patent of invention: application number 201210560096.8).And about the rare report of research that organophosphorus affects haematococcus pulvialis.Major cause: one, inorganic phosphorous sources is considered to the P form that plant dominant absorption utilizes usually; Two, except micromolecular organophosphorus can directly be absorbed by frond, comparatively macromole organophosphorus is then needed just can be absorbed and used [Huang Bangqin after the hydrolysis such as alkaline phosphatase, Huang Shiyu, Hong Huasheng, Deng. the regulating and controlling effect [J] of dissolved phosphorus in the change of marine microalgae alkaline phosphatase activity. ocean journal, 1999,21 (1): 55-60].
Recent studies have found that, many plant planktons can both utilize organo phosphorous compounds to originate as nutritive substance, but specific to different its utilizing status of algae strain there are differences [JB Cotner and RG Wetzel. Uptake of dissolved inorganic and organic phosphorus by phytoplankton and baterioplankton [J]. Limnol Oceanogr, 1992,37 (2): 232-243; Yang Weidong, Zhong Na, Liu Jiesheng, etc. different phosphate sources and concentration are to Prorocentrum lima growth and the influence research [J] producing poison. environmental science, 2008,29 (10): 2760-2765; Wang Yan, Tanghai is molten. and the phosphorus source of different shape is on the impact [J] of phaeocystis globosa growth and alkaline phosphatase. ecological science, 2006,25 (1): 38-40; Qian Shanqin, Kong Fanxiang, Shi little Li etc. the impact [J] that different shape phosphoric acid salt grows microcystic aeruginosa and Chlorella pyrenoidesa. lake science, 2008,20 (6): 796-801].Skeletonema Costatum and Prorocentrum donghaiense all not only can utilize inorganic phosphate but also can utilize organo phosphorous compounds growth and breeding, and the effect of organo phosphorous compounds [Zhao Yanfang slightly higher than the effect of inorganic phosphate, Yu Zhiming, Song Xiuxian, Deng the impact on Skeletonema Costatum and growth of Prorocentrum donghaiense and phosphatase activity of. different phosphate sources form, environmental science, 2009,30 (3): 693-699].Metallographic is bright to be found with Zheng Shuofang (2006) research, under certain organic phosphorus sources existence condition, the exponential growth time that microcystic aeruginosa is longer can be maintained, thus [metallographic is bright to be conducive to forming higher biomass, Zheng Shuofang, organophosphorus and inorganic phosphorus on the impact of Growth of Microcystis aeruginosa and dynamic analysis, Research of Environmental Sciences, 2006,19 (5): 40-44].
But so far, there is not yet the research of organic phosphorus sources to haematococcus pulvialis growth, also inorganic phosphorous sources and organic phosphorus sources collocation are not regulated the discussion of the Growth of Cells of haematococcus pulvialis.By carrying out above-mentioned work, finding the suitable organic phosphorus sources concentration promoting haematococcus pulvialis Growth of Cells, can be applicable to undoubtedly produce and accelerating these industry development paces.
Summary of the invention
The culture medium prescription of the present invention's employing is as follows for achieving the above object:
A kind of Growth Medium For Haematococcus Pluvialis, is characterized in that: contain in often liter of substratum:
NaNO
30.30~0.40 g
KNO
3 0.10~0.20 g
MgSO
4·7H
2O 0.07 ~ 0.18 g
Na
2SO
30.03 ~ 0.06 g
C
6H
5Na
3O
7 0.10 ~ 0.30 g
Inorganic phosphorus 0.02 g
Organophosphorus 0.005 ~ 0.02 g
CaCl
2 0.03 ~ 0.09 g
EDTA-NaFe 0.0002 ~ 0.0003 g
FeSO
40.001 ~ 0.003 g
ZnSO
4 0.0015 ~ 0.0045 g
MnCl
20.00006 ~ 0.00018 g
CoCl
20.00003 ~ 0.0001 g
H
3BO
3 0.0001 ~ 0.0003 g
CuSO
4 0.00006 ~ 0.00018 g
(NH
4)Mo
7O
24 0.00002 ~ 0.00006 g
3-IBA 0.002 g
6-BA 0.0003 g
Vb
120.000025~0.00005 g。
As preference:
Described inorganic phosphorus is KH
2pO
4.
Described organophosphorus is β Sodium Glycerophosphate (G-P), adenosine disodium triphosphate (ATP) or G6P (G-6-P) mixture of one or more wherein.
The mass ratio of described organophosphorus/(organophosphorus+inorganic phosphorus) is 20% ~ 50%.
The mass ratio of described organophosphorus/(organophosphorus+inorganic phosphorus) is 42% ~ 50%.
The Haematocoocus Pluvialls that the present invention cultivates makes it enter Exponential growth stage fast in the green stage of moving about, specific growth rate reaches 0.49 ~ 0.53, the more traditional substratum of biomass improves more than 1 times, effectively raises the output of Haematocoocus Pluvialls at green stage, has broad application prospects.
Substratum of the present invention is used for photoautotrophy and cultivates Haematocoocus Pluvialls, significantly can promote the growth of Haematocoocus Pluvialls, Haematocoocus Pluvialls can be made to enter exponential phase of growth rapidly, reach higher stand density at short notice and keep the superiority of its population, thus contribute at utmost reducing external miscellaneous bacteria, assorted algae and protozoic pollution probability in the wild in enlarged culturing process, shorten growth cycle, improve the chance of success of cultivating.
Accompanying drawing explanation
Fig. 1 is embodiment 1, use the present invention to cultivate Haematocoocus Pluvialls in embodiment 2, embodiment 3, embodiment 4 after growth curve chart;
Fig. 2 is embodiment 5, use the present invention to cultivate Haematocoocus Pluvialls in embodiment 6, embodiment 7, embodiment 8 after growth curve chart.
Embodiment
The present invention is illustrated further by following unrestriced embodiment, but is not used to limit the scope of the invention.
Embodiment 1
(1) contain in often liter of substratum
NaNO
30.32 g、KNO
30.17 g、MgSO
4·7H
2O 0.07g、Na
2SO
30.032g、C
6H
5Na
3O
70.11 g、KH
2PO
40.02 g、G-P 0.015 g、CaCl
20.03 g、EDTANaFe 0.0002 g、FeSO
40.001 g、ZnSO
40.0015 g、MnCl
20.00006 g、CoCl
20.00003 g、H
3BO
30.0001 g、CuSO
40.00006 g、(NH
4)Mo
7O
240.00002 g、3-IBA 0.002 g、6-BA 0.0003 g、Vb
120.000025~0.00005 g。
(2) in 250 mL triangular flasks, add the above-mentioned shown substratum of 100 mL, inoculative proportion is that 10%(and seed liquor volume account for 10% of volume of culture), seed is the haematococcus pulvialis algae strain I of logarithmic phase, and inoculum density is 10
3~ 10
4cells/mL.Culture temperature is (23 ± 1.5) DEG C, cultivates light intensity 1300-1500 lx, and continuous illumination, in cultivation room quiescent culture, is shaken for several times, every Setup Experiments three repetition every day period.
Between incubation period, the nutrient solution plant plankton counting frame that takes a morsel from culturing bottle for every 2 days counts haematococcus pluvialis cell and uses spectrophotometric determination substratum in the optical density(OD) of 680nm, determine the growth phase of frustule, when hot ball frustule quantity or OD680 stablize, complete cultivation.
The SM simultaneously arranging BG11 and improvement is control group (Fig. 1).
Result: all can reach more than 86% in whole culture cycle Green swarm cell ratio, the average specific growth rate of (first 12 days) before plateau relatively in, the specific growth rate of this example reaches 0.515(Fig. 1), be significantly higher than conventional medium BG11(0.368) and improvement SM substratum (0.363).
Embodiment 2
(1) contain in often liter of substratum
NaNO
30.35 g、KNO
30.15 g、MgSO
4·7H
2O 0.14 g、Na
2SO
30.05 g、C
6H
5Na
3O
70.20 g、KH
2PO
40.02 g、G-P 0.010 g、CaCl
20.06 g、EDTANaFe 0.00025 g、FeSO
40.002 g、ZnSO
40.0030 g、MnCl
20.00012 g、CoCl
20.00007 g、H
3BO
30.0002 g、CuSO
40.00012 g、(NH
4)Mo
7O
240.00004 g、3-IBA 0.002 g、6-BA 0.0003 g、Vb
120.000025~0.00005 g。
(2) in 250 mL triangular flasks, add the above-mentioned shown substratum of 100 mL, inoculative proportion is that 10%(and seed liquor volume account for 10% of volume of culture), seed is the haematococcus pulvialis algae strain I of logarithmic phase, and inoculum density is 10
3~ 10
4cells/mL.Culture temperature is (23 ± 1.5) DEG C, cultivates light intensity 1300-1500 lx, and continuous illumination, in cultivation room quiescent culture, is shaken for several times, every Setup Experiments three repetition every day period.
Between incubation period, the nutrient solution plant plankton counting frame that takes a morsel from culturing bottle for every 2 days counts haematococcus pluvialis cell and uses spectrophotometric determination substratum in the optical density(OD) of 680nm, determine the growth phase of frustule, when hot ball frustule quantity or OD680 stablize, complete cultivation.
The SM simultaneously arranging BG11 and improvement is control group (Fig. 1).
Result: all can reach more than 86% in whole culture cycle Green swarm cell ratio, the average specific growth rate of (first 12 days) before plateau relatively in, the specific growth rate of this example reaches 0.506(Fig. 1), be significantly higher than conventional medium BG11(0.368) and improvement SM substratum (0.363).
Embodiment 3
(1) contain in often liter of substratum
NaNO
30.34 g、KNO
30.16 g、MgSO
4·7H
2O 0.13 g、Na
2SO
30.045 g、C
6H
5Na
3O
70.22 g、KH
2PO
40.02 g、G-P 0.02 g、CaCl
20.07 g、EDTANaFe 0.00024 g、FeSO
40.0018 g、ZnSO
40.0024 g、MnCl
20.00015 g、CoCl
20.00005 g、H
3BO
30.00019 g、CuSO
40.00014 g、(NH
4)Mo
7O
240.00003 g、3-IBA 0.002 g、6-BA 0.0003 g、Vb
120.000025~0.00005 g。
(2) in 250 mL triangular flasks, add the above-mentioned shown substratum of 100 mL, inoculative proportion is that 10%(and seed liquor volume account for 10% of volume of culture), seed is the haematococcus pulvialis algae strain I of logarithmic phase, and inoculum density is 10
3~ 10
4cells/mL.Culture temperature is (23 ± 1.5) DEG C, cultivates light intensity 1300-1500 lx, and continuous illumination, in cultivation room quiescent culture, is shaken for several times, every Setup Experiments three repetition every day period.
Between incubation period, the nutrient solution plant plankton counting frame that takes a morsel from culturing bottle for every 2 days counts haematococcus pluvialis cell and uses spectrophotometric determination substratum in the optical density(OD) of 680nm, determine the growth phase of frustule, when hot ball frustule quantity or OD680 stablize, complete cultivation.
The SM simultaneously arranging BG11 and improvement is control group (Fig. 1).
Result: all can reach more than 89% in whole culture cycle Green swarm cell ratio, the average specific growth rate of (first 12 days) before plateau relatively in, the specific growth rate of this example reaches 0.527(Fig. 1), be significantly higher than conventional medium BG11(0.368) and improvement SM substratum (0.363).
Embodiment 4
(1) contain in often liter of substratum
NaNO
30.40 g、KNO
30.20 g、MgSO
4·7H
2O 0.18 g、Na
2SO
30.06 g、C
6H
5Na
3O
70.30 g、KH
2PO
40.02 g、G-P 0.005 g、CaCl
20.09 g、EDTANaFe 0.0003 g、FeSO
40.003 g、ZnSO
40.0045 g、MnCl
20.00018 g、CoCl
20.0001 g、H
3BO
30.0003 g、CuSO
40.00018 g、(NH
4)Mo
7O
240.00006 g、3-IBA 0.002 g、6-BA 0.0003 g、Vb
120.000025~0.00005 g。
(2) in 250 mL triangular flasks, add the above-mentioned shown substratum of 100 mL, inoculative proportion is that 10%(and seed liquor volume account for 10% of volume of culture), seed is the haematococcus pulvialis algae strain I of logarithmic phase, and inoculum density is 10
3~ 10
4cells/mL.Culture temperature is (23 ± 1.5) DEG C, cultivates light intensity 1300-1500 lx, and continuous illumination, in cultivation room quiescent culture, is shaken for several times, every Setup Experiments three repetition every day period.
Between incubation period, the nutrient solution plant plankton counting frame that takes a morsel from culturing bottle for every 2 days counts haematococcus pluvialis cell and uses spectrophotometric determination substratum in the optical density(OD) of 680nm, determine the growth phase of frustule, when hot ball frustule quantity or OD680 stablize, complete cultivation.
The SM simultaneously arranging BG11 and improvement is control group (Fig. 1).
Result: all can reach more than 83% in whole culture cycle Green swarm cell ratio, the average specific growth rate of (first 12 days) before plateau relatively in, the specific growth rate of this example reaches 0.492(Fig. 1), be significantly higher than conventional medium BG11(0.368) and improvement SM substratum (0.363).
Embodiment 5
(1) contain in often liter of substratum
NaNO
30.32 g、KNO
30.17 g、MgSO
4·7H
2O 0.07g、Na
2SO
30.032g、C
6H
5Na
3O
70.11 g、KH
2PO
40.02 g、G-P 0.01 g、ATP 0.005g、CaCl
20.03 g、EDTANaFe 0.0002 g、FeSO
40.001 g、ZnSO
40.0015 g、MnCl
20.00006 g、CoCl
20.00003 g、H
3BO
30.0001 g、CuSO
40.00006 g、(NH
4)Mo
7O
240.00002 g、3-IBA 0.002 g、6-BA 0.0003 g、Vb
120.000025~0.00005 g。
(2) in 250 mL triangular flasks, add the above-mentioned shown substratum of 100 mL, inoculative proportion is that 10%(and seed liquor volume account for 10% of volume of culture), seed is the haematococcus pulvialis algae strain II of logarithmic phase, and inoculum density is 10
3~ 10
4cells/mL.Culture temperature is (23 ± 1.5) DEG C, cultivates light intensity 1300-1500 lx, and continuous illumination, in cultivation room quiescent culture, is shaken for several times, every Setup Experiments three repetition every day period.
Between incubation period, the nutrient solution plant plankton counting frame that takes a morsel from culturing bottle for every 2 days counts haematococcus pluvialis cell and uses spectrophotometric determination substratum in the optical density(OD) of 680nm, determine the growth phase of frustule, when hot ball frustule quantity or OD680 stablize, complete cultivation.
The SM simultaneously arranging BG11 and improvement is control group (Fig. 2).
Result: all can reach more than 87% in whole culture cycle Green swarm cell ratio, the average specific growth rate of (first 12 days) before plateau relatively in, the specific growth rate of this example reaches 0.554(Fig. 2), be significantly higher than conventional medium BG11(0.370) and improvement SM substratum (0.392).
Embodiment 6
(1) contain in often liter of substratum
NaNO
30.35 g、KNO
30.10 g、MgSO
4·7H
2O 0.14 g、Na
2SO
30.05 g、C
6H
5Na
3O
70.20 g、KH
2PO
40.02 g、ATP 0.01g、CaCl
20.06 g、EDTANaFe 0.00025 g、FeSO
40.002 g、ZnSO
40.0030 g、MnCl
20.00012 g、CoCl
20.00007 g、H
3BO
30.0002 g、CuSO
40.00012 g、(NH
4)Mo
7O
240.00004 g、3-IBA 0.002 g、6-BA 0.0003 g、Vb
120.000025~0.00005 g。
(2) in 250 mL triangular flasks, add the above-mentioned shown substratum of 100 mL, inoculative proportion is that 10%(and seed liquor volume account for 10% of volume of culture), seed is the haematococcus pulvialis algae strain II of logarithmic phase, and inoculum density is 10
3~ 10
4cells/mL.Culture temperature is (23 ± 1.5) DEG C, cultivates light intensity 1300-1500 lx, and continuous illumination, in cultivation room quiescent culture, is shaken for several times, every Setup Experiments three repetition every day period.
Between incubation period, the nutrient solution plant plankton counting frame that takes a morsel from culturing bottle for every 2 days counts haematococcus pluvialis cell and uses spectrophotometric determination substratum in the optical density(OD) of 680nm, determine the growth phase of frustule, when hot ball frustule quantity or OD680 stablize, complete cultivation.
The SM simultaneously arranging BG11 and improvement is control group (Fig. 2).
Result: all can reach more than 84% in whole culture cycle Green swarm cell ratio, the average specific growth rate of (first 12 days) before plateau relatively in, the specific growth rate of this example reaches 0.522(Fig. 2), be significantly higher than conventional medium BG11(0.370) and improvement SM substratum (0.392).
Embodiment 7
(1) contain in often liter of substratum
NaNO
30.34 g、KNO
30.16 g、MgSO
4·7H
2O 0.13 g、Na
2SO
30.045 g、C
6H
5Na
3O
70.22 g、KH
2PO
40.02 g、G-P 0.01 g、ATP 0.005g、G-6-P 0.005g、CaCl
20.07 g、EDTANaFe 0.00024 g、FeSO
40.0018 g、ZnSO
40.0024 g、MnCl
20.00015 g、CoCl
20.00005 g、H
3BO
30.00019 g、CuSO
40.00014 g、(NH
4)Mo
7O
240.00003 g、3-IBA 0.002 g、6-BA 0.0003 g、Vb
120.000025~0.00005 g。
(2) in 250 mL triangular flasks, add the above-mentioned shown substratum of 100 mL, inoculative proportion is that 10%(and seed liquor volume account for 10% of volume of culture), seed is the haematococcus pulvialis algae strain II of logarithmic phase, and inoculum density is 10
3~ 10
4cells/mL.Culture temperature is (23 ± 1.5) DEG C, cultivates light intensity 1300-1500 lx, and continuous illumination, in cultivation room quiescent culture, is shaken for several times, every Setup Experiments three repetition every day period.
Between incubation period, the nutrient solution plant plankton counting frame that takes a morsel from culturing bottle for every 2 days counts haematococcus pluvialis cell and uses spectrophotometric determination substratum in the optical density(OD) of 680nm, determine the growth phase of frustule, when hot ball frustule quantity or OD680 stablize, complete cultivation.
The SM simultaneously arranging BG11 and improvement is control group (Fig. 2).
Result: all can reach more than 86% in whole culture cycle Green swarm cell ratio, the average specific growth rate of (first 12 days) before plateau relatively in, the specific growth rate of this example reaches 0.563(Fig. 2), be significantly higher than conventional medium BG11(0.370) and improvement SM substratum (0.392).
Embodiment 8
(1) contain in often liter of substratum
NaNO
30.40 g、KNO
30.20 g、MgSO
4·7H
2O 0.18 g、Na
2SO
30.06 g、C
6H
5Na
3O
70.30 g、KH
2PO
40.02 g、G-6-P 0.005 g、CaCl
20.09 g、EDTANaFe 0.0003 g、FeSO
40.003 g、ZnSO
40.0045 g、MnCl
20.00018 g、CoCl
20.0001 g、H
3BO
30.0003 g、CuSO
40.00018 g、(NH
4)Mo
7O
240.00006 g、3-IBA 0.002 g、6-BA 0.0003 g、Vb
120.000025~0.00005 g。
(2) in 250 mL triangular flasks, add the above-mentioned shown substratum of 100 mL, inoculative proportion is that 10%(and seed liquor volume account for 10% of volume of culture), seed is the haematococcus pulvialis algae strain II of logarithmic phase, and inoculum density is 10
3~ 10
4cells/mL.Culture temperature is (23 ± 1.5) DEG C, cultivates light intensity 1300-1500 lx, and continuous illumination, in cultivation room quiescent culture, is shaken for several times, every Setup Experiments three repetition every day period.
Between incubation period, the nutrient solution plant plankton counting frame that takes a morsel from culturing bottle for every 2 days counts haematococcus pluvialis cell and uses spectrophotometric determination substratum in the optical density(OD) of 680nm, determine the growth phase of frustule, when hot ball frustule quantity or OD680 stablize, complete cultivation.
The SM simultaneously arranging BG11 and improvement is control group (Fig. 2).
Result: all can reach more than 83% in whole culture cycle Green swarm cell ratio, the average specific growth rate of (first 12 days) before plateau relatively in, the specific growth rate of this example reaches 0.520(Fig. 2), be significantly higher than conventional medium BG11(0.370) and improvement SM substratum (0.392).
Above embodiment is used for illustrative purposes only, but not limitation of the present invention, person skilled in the relevant technique; when not departing from the spirit and scope of invention; can also make various conversion, therefore all equivalent technical schemes, all fall into protection scope of the present invention.
Claims (4)
1. a Growth Medium For Haematococcus Pluvialis, is characterized in that: contain in often liter of substratum:
NaNO
30.30~0.40 g
KNO
3 0.10~0.20 g
MgSO
4·7H
2O 0.07 ~ 0.18 g
Na
2SO
30.03 ~ 0.06 g
C
6H
5Na
3O
7 0.10 ~ 0.30 g
Inorganic phosphorus 0.02 g
Organophosphorus 0.005 ~ 0.02 g
CaCl
2 0.03 ~ 0.09 g
EDTA-NaFe 0.0002 ~ 0.0003 g
FeSO
40.001 ~ 0.003 g
ZnSO
4 0.0015 ~ 0.0045 g
MnCl
20.00006 ~ 0.00018 g
CoCl
20.00003 ~ 0.0001 g
H
3BO
3 0.0001 ~ 0.0003 g
CuSO
4 0.00006 ~ 0.00018 g
(NH
4)Mo
7O
24 0.00002 ~ 0.00006 g
3-IBA 0.002 g
6-BA 0.0003 g
Vb
120.000025~0.00005 g；
Described organophosphorus is the mixture of β Sodium Glycerophosphate, adenosine disodium triphosphate or G6P wherein one or more.
2. a kind of Growth Medium For Haematococcus Pluvialis as claimed in claim 1, is characterized in that: described inorganic phosphorus is KH
2pO
4.
3. a kind of Growth Medium For Haematococcus Pluvialis as claimed in claim 1, is characterized in that: the mass ratio of described organophosphorus/(organophosphorus+inorganic phosphorus) is 20% ~ 50%.
4. a kind of Growth Medium For Haematococcus Pluvialis as claimed in claim 1, is characterized in that: the mass ratio of described organophosphorus/(organophosphorus+inorganic phosphorus) is 42% ~ 50%.
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| CN103966103A (en) * | 2014-05-26 | 2014-08-06 | 临沂大学 | Culture medium and culture method for culturing haematococcus pluvialis by using brewery wastewater |
| CN105567625A (en) * | 2016-02-26 | 2016-05-11 | 中国水产科学研究院长江水产研究所 | Haematococcus pluvialis induction medium |
| CN109294919A (en) * | 2018-10-17 | 2019-02-01 | 云南博欣生物科技股份有限公司 | A kind of algae update purification process of haematococcus pluvialis |
| EP4036217A4 (en) * | 2019-09-23 | 2023-11-01 | Bioalgo (WF) Co., Ltd | Method for producing astaxanthin by heterotrophic culture of haematococcus pluvialis |
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| CN101144058A (en) * | 2007-08-22 | 2008-03-19 | 厦门大学 | Micro-algae culture medium for astaxanthin |
| CN103114121A (en) * | 2013-01-31 | 2013-05-22 | 宁波大学 | Method for producing astaxanthin by haematococcus pluvialis |
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| CN101144058A (en) * | 2007-08-22 | 2008-03-19 | 厦门大学 | Micro-algae culture medium for astaxanthin |
| CN103114121A (en) * | 2013-01-31 | 2013-05-22 | 宁波大学 | Method for producing astaxanthin by haematococcus pluvialis |
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| Title |
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| Commercial production of astaxanthin from Haematococcus pluvialis using 25,000-liter outdoor photobioreactors;Miguel Olaizola;《Journal of Applied Phycology》;20001031;第12卷(第3-5期);499-506 * |
| Two-stage cultures for the production of Astaxanthin from Haematococcus pluvialis;Jaime Fábregas等;《Journal of Biotechnology》;20010726;第89卷(第1期);65-71 * |
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