CN103114121A - Method for producing astaxanthin by haematococcus pluvialis - Google Patents

Abstract

The invention discloses a method for producing astaxanthin by haematococcus pluvialis. The method comprises the steps of: firstly, naturally culturing the haematococcus pluvialis in water of a culture pool for 6-8 days so that the haematococcus pluvialis proliferates, then adding potassium dihydrogen phosphate and sodium nitrate to the pool water, and covering the pool with a red thin film, so that algae cells of the haematococcus pluvialis rapidly grow to reach conversion state under the irradiation of 630nm-760nm light waves; transferring the algae cells at the conversion state to pool water with potassium dihydrogen phosphate, the final concentration of which is 0.02-0.05g/L, covering the pool with a blue thin film, so that the algae cells of haematococcus pluvialis are cultivated to mature under the irradiation of 430nm-490nm light waves, and thus obtaining haematococcus pluvialis, the algae cells of which are rich in astaxanthin, wherein potassium dihydrogen phosphate, sodium nitrate and red lights can enable the algae cells to rapidly grow and reach conversion state in shorter time, and the potassium dihydrogen phosphate and blue lights can enable the algae cells to rapidly grow, mature and accumulate astaxanthin. The method has the advantages of little investment, cultivation simpleness and high economic benefits.

Description

A kind of method of haematococcus pluvialis to produce astaxanthin

Technical field

The present invention relates to the production method of astaxanthin, be specifically related to a kind of method of haematococcus pluvialis to produce astaxanthin.

Background technology

Haematocoocus Pluvialls ( Haematococcus pluvialis) be a kind of unicell green alga, be the present known the highest biology of content astaxanthin, Haematocoocus Pluvialls can up to 1.5%～3.0% of dry weight, be regarded as " concentrate " of natural astaxanthin because self accumulating the astaxanthin material.Utilize at present the method for haematococcus pluvialis to produce astaxanthin varied, as the patent of invention of Granted publication CN1181184, utilize complicated nutrient chemical, enlarge growth in bio-reactor, be converted into astaxanthin, reach the Accumulation of Astaxanthin purpose; Granted publication number is the patent of invention of CN1004536325, utilizes complexes, in the suitable nutrient solution of the content of nitrogen and phosphorous, controls with LED light source Haematocoocus Pluvialls is enlarged growth, is converted into astaxanthin; These two kinds of input costs are high, and adjusting and controlling growth is complicated, and economic benefit is lower.Granted publication number is the CN101586140 patent of invention, discloses in the content of nitrogen and phosphorous nutrient solution, logically controls different pH values, culture temperature, intensity of illumination, light application time, add rock salt what cultivate, realize growth and conversion, then add H ₂O ₂, FeCl ₂Cultivate the accumulation astaxanthin, input cost is high, cultivates complexity, and economic benefit is lower.

Summary of the invention

Technical problem to be solved by this invention is to provide a kind of less investment, cultivates simply the method with haematococcus pluvialis to produce astaxanthin that economic benefit is higher.Present method is used the illumination of varying strength and color and luster by different times, reach breeding and the conversion of Haematocoocus Pluvialls, has less investment, cultivates simply, and economic benefit is higher.

The present invention solves the problems of the technologies described above the technical scheme that adopts: a kind of method of haematococcus pluvialis to produce astaxanthin, and its step is as follows:

A, be not more than the density of 50,000 frustules with every milliliter of Chi Shui, Haematocoocus Pluvialls being put into the pond water of cultivation pool cultivated 6～8 days naturally, then add potassium primary phosphate and SODIUMNITRATE in pool water within, in the water of described pond, the potassium primary phosphate final concentration is 0.02～0.05 grams per liter, the SODIUMNITRATE final concentration is 0. 2～0. 5 grams per liters, be coated with red film above described cultivation pool, Chi Shui is under 630nm～760nm light-wave irradiation at wavelength, Haematocoocus Pluvialls is cultured to transforms the attitude frustule, obtain transforming the Haematocoocus Pluvialls of attitude frustule;

B, the Haematocoocus Pluvialls that will transform the attitude frustule move into the Chi Shuizhong of conversion pool, contain the potassium primary phosphate that final concentration is 0.02～0.05 grams per liter in the water of described pond, be coated with blue membrane above described conversion pool, Chi Shui is under 430nm～490nm light-wave irradiation at wavelength, Haematocoocus Pluvialls is cultured to maturation, obtains the Haematocoocus Pluvialls that frustule is rich in astaxanthin.

Compared with prior art, the invention has the advantages that: a kind of method of haematococcus pluvialis to produce astaxanthin, first Haematocoocus Pluvialls was cultivated 6～8 days in the water of cultivation pool pond naturally, allow its propagation, add again potassium primary phosphate and SODIUMNITRATE in pool water within, and being covered with red film on the pond, wavelength is under 630nm～760nm light-wave irradiation, allows Haematocoocus Pluvialls frustule Fast Growth to transforming attitude; Transform the attitude frustule and containing the Chi Shuizhong that final concentration is the potassium primary phosphate of 0.02～0.05 grams per liter, and be covered with blue membrane on the pond, wavelength is under 430nm～490nm light-wave irradiation, allows the Haematocoocus Pluvialls frustule support to ripe, obtains the Haematocoocus Pluvialls that frustule is rich in astaxanthin; Potassium primary phosphate, SODIUMNITRATE and ruddiness can make the frustule Fast Growth, and the short period reaches the conversion attitude; Potassium primary phosphate, blue light can make frustule Fast Growth maturation and accumulation astaxanthin; Red film can reflect red, and blue membrane can reflect blue, irradiation effect is improved, and improve the temperature of Chi Shui, accelerates growth, transforms and ripe.The method less investment is cultivated simply, and economic benefit is higher.

Embodiment

Below in conjunction with embodiment, the present invention is described in further detail.

Embodiment 1

Haematocoocus Pluvialls is put into the Chi Shuizhong of cultivation pool, it is 40,000 frustule/milliliters that Chi Shuizhong puts into Haematocoocus Pluvialls density, first cellar culture 6～8 days, this moment, Haematocoocus Pluvialls density increased, about 240,000 frustule/milliliters, then add potassium primary phosphate and SODIUMNITRATE in pool water within, make that in the water of pond, the potassium primary phosphate final concentration is 0.02～0.05 grams per liter, the SODIUMNITRATE final concentration is 0. 2～0. 5 grams per liters, be coated with red film on cultivation pool, Chi Shui is under 630nm～760nm light-wave irradiation at wavelength, after 7～8 days, Haematocoocus Pluvialls is cultured to and transforms the attitude frustule, obtain transforming the Haematocoocus Pluvialls of attitude frustule, the Haematocoocus Pluvialls that will transform again the attitude frustule moves into the Chi Shuizhong of conversion pool, this Chi Shuizhong contains the potassium primary phosphate that final concentration is 0.02 grams per liter, be coated with blue membrane above conversion pool, Chi Shui is under 430nm～490nm light-wave irradiation at wavelength, Haematocoocus Pluvialls is cultured to maturation, obtain the Haematocoocus Pluvialls that frustule is rich in astaxanthin, the content of astaxanthin is about 1.2%.

Embodiment 2

Substantially the same manner as Example 1, when difference is just put into, Haematocoocus Pluvialls density is 50,000 frustule/milliliters, and algae density was about 270,000 frustule/milliliters in 6～8 days; In the water of conversion pool pond, the potassium primary phosphate final concentration is 0.05 grams per liter, and the content of astaxanthin is about 1.7%.

Claims (1)

1. the method for a haematococcus pluvialis to produce astaxanthin is characterized in that step is as follows:

A, be not more than the density of 50,000 frustules with every milliliter of Chi Shui, Haematocoocus Pluvialls being put into the pond water of cultivation pool cultivated 6～8 days naturally, then add potassium primary phosphate and SODIUMNITRATE in pool water within, in the water of described pond, the potassium primary phosphate final concentration is 0.02～0.05 grams per liter, the SODIUMNITRATE final concentration is 0. 2～0. 5 grams per liters, be coated with red film above described cultivation pool, Chi Shui is under 630nm～760nm light-wave irradiation at wavelength, Haematocoocus Pluvialls is cultured to transforms the attitude frustule, obtain transforming the Haematocoocus Pluvialls of attitude frustule;

B, the Haematocoocus Pluvialls that will transform the attitude frustule move into the Chi Shuizhong of conversion pool, contain the potassium primary phosphate that final concentration is 0.02～0.05 grams per liter in the water of described pond, be coated with blue membrane above described conversion pool, Chi Shui is under 430nm～490nm light-wave irradiation at wavelength, Haematocoocus Pluvialls is cultured to maturation, obtains the Haematocoocus Pluvialls that frustule is rich in astaxanthin.