CN102337215A - Methods for culturing haematococcus pluvialis and producing astaxanthin - Google Patents
Methods for culturing haematococcus pluvialis and producing astaxanthin Download PDFInfo
- Publication number
- CN102337215A CN102337215A CN2011103197681A CN201110319768A CN102337215A CN 102337215 A CN102337215 A CN 102337215A CN 2011103197681 A CN2011103197681 A CN 2011103197681A CN 201110319768 A CN201110319768 A CN 201110319768A CN 102337215 A CN102337215 A CN 102337215A
- Authority
- CN
- China
- Prior art keywords
- light source
- astaxanthin
- haematocoocus pluvialls
- haematococcus pluvialis
- temperature
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/54—Improvements relating to the production of bulk chemicals using solvents, e.g. supercritical solvents or ionic liquids
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Cultivation Of Plants (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention provides a haematococcus pluvialis culture production method and a method for further producing astaxanthin. The haematococcus pluvialis culture process is carried out in a large-size container, and haematococcus pluvialis is cultured under the irradiation of an artificial LED (light-emitting diode) light source; the artificial LED light source is provided by a high-brightness LED, the wavelength range of single-color light emitted by the LED light source is from 450nm (width band of 30nm) to 640nm (width band of 30nm); the used illumination intensity is 30mu E/m<2>.s-3000mu E/m<2>.s; in the culture process, cells are maintained to be suspended in the culture liquid in utilizing a ventilation mode, and temperature is maintained to be 15-28 DEG C; and the pH value is maintained to be 6.8-8.5. According to the invention, on one hand, more nutrient substances are accumulated so as to accelerate growth and reproduction; on the other hand, the problem that the temperature is difficult to control due to the adoption of a natural sun light source is solved, thereby saving a large amount of energy consumed for controlling the temperature in summer and winter, reducing production cost and environmental pollution; and simultaneously, according to the characteristic that photosynthesis pigments demand different light qualities, the most effective single-color diode light source is adopted, thereby improving energy utilization rate, production efficiency and cell concentration.
Description
Technical field
The present invention relates to technical scale and cultivate the method for Haematocoocus Pluvialls, the invention still further relates to the method for industrial-scale production astaxanthin.Described Haematocoocus Pluvialls belongs to fresh water algae.
Background technology
Haematocoocus Pluvialls is called the lake again and gives birth to haematococcus pulvialis or lake green blood ball algae, belongs to volvocales, haematococcus pulvialis section.
Find that in 1899 this algae often has on the Repulse Bay limit of water attached to the water altar or by extra large periodicity with a kind of form of blood red shell; The life course of this algae is the dormant stage through a redness; Being moving about the stage of a green afterwards, is again the dormant stage of a redness more afterwards.
1934, Elliot replenished the details of this algae grows history from the angle of morphocytology.Four kinds of typical cellular fories appear in whole life: microzooid, long big polypide amphitrichous, do not have motor capacity glue sheath body, have red maxicell---the red sporangiocyst (Haematocysts) of sclereid wall.In the cleaning ambient of sufficient nutrition was arranged, big polypide was occupied an leading position; Be glue sheath body in case environmental degradation will be converted into, be converted into red sporangiocyst afterwards, and begin to accumulate astaxanthin with resistibility.Subsequently, when around environment become nutritional sufficiency again suitable the time, red sporangiocyst becomes movable microzooid, these microzooids grow up to glue sheath body or big polypide.
Content astaxanthin is 1.5%～3.0% in the Haematocoocus Pluvialls, is counted as " concentrate " of natural astaxanthin.Haematocoocus Pluvialls is high than other green alga to the accumulation rate and the production of astaxanthin; And proportioning (about 70% the monoesters of Haematocoocus Pluvialls institute's astaxanthin-containing and ester class thereof; 25% dibasic acid esters and 5% monomer) very similar with aquatic animal self proportioning, this is not available through chemosynthesis and the astaxanthin that utilizes phaffiafhodozyma etc. to extract.In addition, the structure of astaxanthin is main with 3S-3 ' S type in the Haematocoocus Pluvialls, with astaxanthin structure basically identical in the water generates objects such as salmon; The astaxanthin structure then is 3R 1 ' R type in the phaffiafhodozyma.Current, Haematocoocus Pluvialls is acknowledged as the best biology that occurring in nature is produced natural astaxanthin, therefore, utilizes this little algae to extract astaxanthin and has vast potential for future development undoubtedly, has become in recent years the research focus of natural astaxanthin production in the world.In the document relevant, has no virose report with haematococcus pulvialis.
Haematocoocus Pluvialls is on November 11st, 2010 " No. 17 bulletin of Ministry of Health of the People's Republic of China ", obtaining " birth approval certificate ".From then in the inhibitor industry, the production of natural astaxanthin and research and development thereof will get into the motorway, and natural astaxanthin will be to oxidative stress class disease, produce revolutionary change like the control of mellitus, gout, hypertension etc.
Compare with little algae of the mankind such as tenaculat Habenaria, chlorella health care commonly used, Haematocoocus Pluvialls has more amazing antioxygenation, and more highly difficult breed and preservation technology are also arranged simultaneously.
This fresh water algae of Haematocoocus Pluvialls is different from marine alga, and the latter is mainly contained chlorophyll a and chlorophyll b, and the Photosynthesis Pigment that contains in the haematococcus pluvialis cell mainly is chlorophyll a and astaxanthin.This fresh water algae of Haematocoocus Pluvialls is different from one of characteristic of marine alga and other fresh water algae: be made up of green and red two stages its life history.
Another characteristic that this fresh water algae of Haematocoocus Pluvialls is different from marine alga and other fresh water algae is: content astaxanthin is low in green stage cell, and reproduction speed is fast, a little less than the light requirement, and not anti-high temperature more than 28 ℃; And high at red stage content astaxanthin, breeding is slow, and growth is fast, likes high illumination, anti-28 ℃ to 36 ℃ relatively-high temperature.
Therefore, the cultivation of Haematocoocus Pluvialls is difficulty relatively, culture condition such as temperature and illumination is required very harsh.The natural lighting method that adopts mostly in the present research and production is difficult to accomplish accurate temperature controlling.Particularly in summer and winter, a large amount of energy is wasted in the cooling in summer and the heat temperature raising in winter.
Summary of the invention
Technical problem to be solved by this invention is a kind of Haematocoocus Pluvialls working method to be provided, under the prerequisite of effective save energy; Through culture condition such as strict controlled temperature and illumination; Make Haematocoocus Pluvialls breeding very fast, further enhancing productivity, and obtain higher cell concn.The present invention also provides a kind of method of utilizing haematococcus pluvialis to produce astaxanthin.
It is following that the present invention solves the problems of the technologies described above the technical scheme that is adopted:
Haematocoocus Pluvialls cultivating and producing method is characterized in that:
Culturing process is carried out in tun, adopts artificial led light source that the Haematocoocus Pluvialls irradiation is cultivated;
The monochromatic wavelength region that this led light source sends is at 450nm bandwidth 30nm and 640nm bandwidth 30nm, preferred 475nm and 663nm; Use the light source of two kinds of above-mentioned different wave lengths simultaneously, total intensity of illumination is 30 μ E/m
2.s～3000 μ E/m
2.s, preferred 500～2000 μ E/m
2.s; Led light source light application time 6～24h/ days, preferred 12-～24 h/skies;
Culturing process utilizes ventilating mode to make cell in nutrient solution, keep suspending, and temperature remains on 15～28 ℃, preferred 23-26 ℃; PH remains on 6.8～8.5.
Utilize the method for haematococcus pluvialis to produce astaxanthin, it is characterized in that:
The first step: accumulation astaxanthin: in container, adopt artificial led light source that green algae liquid is carried out illumination, the accumulation astaxanthin;
The monochromatic wavelength region that this led light source sends is at 450nm bandwidth 30nm and 640nm bandwidth 30nm, preferred 475nm and 663nm; Use the light source of two kinds of above-mentioned different wave lengths simultaneously, total intensity of illumination is 200 μ E/m
2.s～30000 μ E/m
2.s, preferred 1000 μ E/m
2.s～10000 μ E/m
2.s;
Culturing process makes cell in nutrient solution, keep suspending, and temperature remains on 28～36 ℃, preferred 30～33 ℃;
Second step, the red haematococcus pulvialis of gathering: cultivate and be full of red astaxanthin in the cell in the nutrient solution, algae liquid is dark red
During look, carry out centrifugal or sedimentation is gathered;
The 3rd step, drying: the second step Haematocoocus Pluvialls of gathering is dried to water cut under 100 ℃ the temperature and is lower than being lower than
10%；
The 4th step, broken wall: carry out broken wall treatment with the equal agent method of high pressure pair cell;
The 5th step, extraction astaxanthin: extract astaxanthin with the carbon dioxide upercritical fluid extraction appearance.
Positively effect of the present invention is: the breeding suitable growth in period of Haematocoocus Pluvialls is under the low light level.Therefore the present invention adopts photodiode (LED) to make light source, can make cell carry out photosynthesis in continuous 24 hours on the one hand, accumulation nutritive substance, accelerating growth breeding; Avoided the temperature control difficulty that adopts the nature solar source to bring on the other hand, saved two season of winter in the summer mass energy that consumed of temperature control, reduced production cost and the pollution of environment.According to the characteristics of demand of Photosynthesis Pigment, adopt the most effectively monochrome photodiode light source simultaneously, improved capacity usage ratio, production efficiency, cell concn and astaxanthin productive rate different light medium.
Embodiment
Further specify the present invention below in conjunction with accompanying drawing and embodiment.
The present invention cultivates the method for Haematocoocus Pluvialls, comprises the following steps:
Culturing process is carried out in the tun more than 500 liters,
The first step, reactor drum sterilization: reactor drum is carried out sterilising treatment with chemistry or steaming process.
Second step, selection substratum: adopt the GB11 substratum.
The 3rd step, medium sterilization: substratum is carried out sterilization of hypochlorous acid chemistry or high-temperature sterilization.After the chemosterilization substratum is neutralized to pH6.0-8.5, substratum is cooled to below 28 ℃ behind the high-temperature sterilization.
The 4th step, inoculation and cultivation: the green swarm cell of Haematocoocus Pluvialls is inoculated in the substratum aerobic culture.
Culturing process is carried out in insulating container, adopts artificial led light source to carry out 24 hours uninterrupted illumination cultivation of Haematocoocus Pluvialls.Also can utilize daytime daylight, night to adopt artificial led light source.
Said artificial led light source is provided by high brightness LED, and the monochromatic wavelength region that this led light source sends is at 450nm bandwidth 30nm and 640nm bandwidth 30nm, and preferred monochromatic wavelength is 475nm and 663nm.Use the light source of two kinds of above-mentioned different wave lengths simultaneously.Total intensity of illumination is 30 μ E/m
2.s～3000 μ E/m
2.s, preferred intensity of illumination scope is: 1000 μ E/m
2.s.
Culturing process utilizes ventilating mode to make cell in nutrient solution, keep suspending, and temperature remains on 15～28 ℃, and is preferred
TR is: 23-26 ℃, pH remains on 6.8～8.5.
The 5th step, gather: when cell concn reaches ten thousand/ml of 80-120, carry out centrifugal or sedimentation is gathered.
The Haematocoocus Pluvialls that the 5th step was gathered is green algae liquid.
Compare with traditional cultural method, the outstanding feature of this cultural method is:
The first, through measuring and calculating, the energy consumption of obviously having saved temperature control;
The second, culture cycle has shortened more than 50% than traditional method;
Three, through biochemical analysis, the Haematocoocus Pluvialls cell concn reaches 1,200,000/ml in the resulting green algae liquid, is 2-5 times of traditional method.
The present invention produces the method for astaxanthin, comprises the following steps:
The first step: accumulation astaxanthin: the green algae liquid that will gather changes open runway pond over to and carries out the astaxanthin accumulation cultivation.
Concrete steps are: in container, adopt artificial led light source that green algae liquid is carried out illumination, the accumulation astaxanthin;
The monochromatic wavelength region that this led light source sends is at 450nm bandwidth 30nm and 640nm bandwidth 30nm, preferred 475nm and 663nm.Use the light source of two kinds of above-mentioned different wave lengths simultaneously, total intensity of illumination is 200 μ E/m
2.s～30000 μ E/m
2.s, preferred 1000 μ E/m
2.s～10000 μ E/m
2.s.
Culturing process makes cell in nutrient solution, keep suspending, and temperature remains on 28～36 ℃, and preferably 30～33 ℃, pH7.0～9.0).
Second step, the red Haematocoocus Pluvialls of gathering: cultivate and be full of red astaxanthin in the cell, algae liquid is dark red
During look, carry out centrifugal or sedimentation is gathered.
The 3rd step, broken wall: carry out broken wall treatment with the equal agent method of high pressure pair cell.
The 4th step, drying: the Haematocoocus Pluvialls behind the broken wall is dried to water cut under 100 ℃ the temperature and is lower than 10% being lower than.
The 5th step, extraction astaxanthin: extract astaxanthin with the carbon dioxide upercritical fluid extraction appearance.
Astaxanthin productive rate of the present invention can reach 4500mg/m
2.d, compare with traditional method and be more than doubled.
Claims (9)
1. Haematocoocus Pluvialls cultivating and producing method is characterized in that:
Culturing process is carried out in tun, adopts artificial led light source that the Haematocoocus Pluvialls irradiation is cultivated;
The monochromatic wavelength region that this led light source sends is at 450nm bandwidth 30nm and 640nm bandwidth 30nm; Use the light source of two kinds of above-mentioned different wave lengths simultaneously, total intensity of illumination is 30 μ E/m
2.s～3000 μ E/m
2.s; Led light source light application time 6～24h/ days;
Culturing process utilizes ventilating mode to make cell in nutrient solution, keep suspending, and temperature remains on 15～28 ℃; PH remains on 6.8～8.5.
2. Haematocoocus Pluvialls cultivating and producing method as claimed in claim 1 is characterized in that: the monochromatic wavelength of LED respectively
Be 475nm and 663nm.
3. Haematocoocus Pluvialls cultivating and producing method as claimed in claim 1 is characterized in that: total intensity of illumination is: 500～2000 μ E/m
2.s.
4. Haematocoocus Pluvialls cultivating and producing method as claimed in claim 1, it is characterized in that: the culture temperature scope is: 23-26 ℃.
5. Haematocoocus Pluvialls cultivating and producing method as claimed in claim 1 is characterized in that: led light source light application time 12～24 h/skies.
6. utilize the method for haematococcus pluvialis to produce astaxanthin, it is characterized in that:
The first step: accumulation astaxanthin: in container, adopt artificial led light source that green algae liquid is carried out illumination, the accumulation astaxanthin;
The monochromatic wavelength region that this led light source sends is at 450nm bandwidth 30nm and 640nm bandwidth 30nm; Use the light source of two kinds of above-mentioned different wave lengths simultaneously, total intensity of illumination is 200 μ E/m
2.s～30000 μ E/m
2.s;
Culturing process makes cell in nutrient solution, keep suspending, and temperature remains on 28～36 ℃;
Second step, the red haematococcus pulvialis of gathering: cultivate and be full of red astaxanthin in the cell in the nutrient solution, algae liquid is dark red
During look, carry out centrifugal or sedimentation is gathered;
The 3rd step, drying: the second step Haematocoocus Pluvialls of gathering is dried to water cut under 100 ℃ the temperature and is lower than being lower than
10%；
The 4th step, broken wall: carry out broken wall treatment with the equal agent method of high pressure pair cell;
The 5th step, extraction astaxanthin: extract astaxanthin with the carbon dioxide upercritical fluid extraction appearance.
7. the method for utilizing haematococcus pluvialis to produce astaxanthin as claimed in claim 6 is characterized in that: the monochromatic wavelength of LED is 475nm and 663nm.
8. the method for utilizing haematococcus pluvialis to produce astaxanthin as claimed in claim 6 is characterized in that: total intensity of illumination scope is: 1000 μ E/m
2.s～10000 μ E/m
2.s.
9. the method for utilizing haematococcus pluvialis to produce astaxanthin as claimed in claim 6 is characterized in that: the culturing process temperature
Remain on 30～33 ℃.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011103197681A CN102337215A (en) | 2011-10-20 | 2011-10-20 | Methods for culturing haematococcus pluvialis and producing astaxanthin |
| CN201210295602.5A CN102766578B (en) | 2011-10-20 | 2012-08-20 | Cultivating and producing method for haematococcus pluvialis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011103197681A CN102337215A (en) | 2011-10-20 | 2011-10-20 | Methods for culturing haematococcus pluvialis and producing astaxanthin |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102337215A true CN102337215A (en) | 2012-02-01 |
Family
ID=45513169
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011103197681A Pending CN102337215A (en) | 2011-10-20 | 2011-10-20 | Methods for culturing haematococcus pluvialis and producing astaxanthin |
| CN201210295602.5A Expired - Fee Related CN102766578B (en) | 2011-10-20 | 2012-08-20 | Cultivating and producing method for haematococcus pluvialis |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210295602.5A Expired - Fee Related CN102766578B (en) | 2011-10-20 | 2012-08-20 | Cultivating and producing method for haematococcus pluvialis |
Country Status (1)
| Country | Link |
|---|---|
| CN (2) | CN102337215A (en) |
Cited By (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102604836A (en) * | 2012-03-01 | 2012-07-25 | 武汉凯迪工程技术研究总院有限公司 | Production method and device for preventing chytrid pollution in haematococcus pluvialis |
| CN102766578A (en) * | 2011-10-20 | 2012-11-07 | 烟台华融生物科技有限公司 | Cultivating and producing method for haematococcus pluvialis |
| CN103114121A (en) * | 2013-01-31 | 2013-05-22 | 宁波大学 | Method for producing astaxanthin by haematococcus pluvialis |
| CN104529852A (en) * | 2014-12-10 | 2015-04-22 | 青岛无为保温材料有限公司 | Method for extracting astaxanthin from haematococcus pluvialis |
| CN104531530A (en) * | 2014-12-10 | 2015-04-22 | 青岛无为保温材料有限公司 | Wall breaking method for haematococcus pluvialis |
| CN104856051A (en) * | 2015-04-10 | 2015-08-26 | 杭州鑫伟低碳技术研发有限公司 | Method for producing microcapsules of astaxanthin powder by utilizing haematococcus pluvialis |
| CN104886578A (en) * | 2015-06-15 | 2015-09-09 | 杭州鑫伟低碳技术研发有限公司 | Production device and production method for haematococcus pluvialis micro-capsule conidial powder |
| WO2015151577A1 (en) * | 2014-04-03 | 2015-10-08 | 日本水産株式会社 | Method for producing astaxanthin |
| CN105916993A (en) * | 2014-04-17 | 2016-08-31 | 高丽大学校产学协力团 | Method for increasing production of astaxanthin in haematococcus pluvialis by mature spore inoculation and iron ion-mediated Harber-Weiss reaction at high temperature |
| CN107099458A (en) * | 2017-04-06 | 2017-08-29 | 深圳市德和生物科技有限公司 | A kind of biological respinse kettle and the method for promoting haematococcus pluvialis propagation and redden |
| CN107354122A (en) * | 2017-09-18 | 2017-11-17 | 深圳市德和生物科技有限公司 | A kind of method for promoting haematococcus pluvialis growing multiplication and redden |
| WO2018056160A1 (en) * | 2016-09-21 | 2018-03-29 | 日本水産株式会社 | Method for producing astaxanthin |
| CN108350478A (en) * | 2015-09-11 | 2018-07-31 | 科隆大学 | A method of cultivating the haematococcus biology for manufacturing astaxanthin |
| CN110064029A (en) * | 2019-05-24 | 2019-07-30 | 云南蓝钻生物科技股份有限公司 | A kind of pharmaceutical composition and preparation method thereof for treating gout |
| CN110184168A (en) * | 2019-05-21 | 2019-08-30 | 荆楚理工学院 | A kind of simple astaxanthin incubator and the method using its culture astaxanthin |
| CN112842967A (en) * | 2021-03-05 | 2021-05-28 | 广州正广生物科技有限公司 | Anti-aging composition with function of removing free radicals and preparation method thereof |
| CN112998270A (en) * | 2021-04-12 | 2021-06-22 | 海南三元星生物科技股份有限公司 | Preparation method of astaxanthin microcapsules |
| CN113214998A (en) * | 2021-06-16 | 2021-08-06 | 江苏格局生物医药科技有限公司 | Preparation method of natural astaxanthin |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2952573B1 (en) * | 2013-02-04 | 2017-11-15 | Showa Denko K.K. | Method for promoting growth of green algae |
| EP2952575B1 (en) * | 2013-02-04 | 2018-04-04 | Showa Denko K.K. | Method for promoting growth of green algae |
| JP5658424B1 (en) * | 2013-02-04 | 2015-01-28 | 昭和電工株式会社 | Green algae growth promotion method |
| JP6575987B2 (en) * | 2014-12-17 | 2019-09-18 | 昭和電工株式会社 | Green algae culture method and astaxanthin production method |
| CN105638431B (en) * | 2015-12-31 | 2019-04-02 | 浙江大学 | A kind of efficient algae culturing equipment |
| WO2017113285A1 (en) * | 2015-12-31 | 2017-07-06 | 浙江大学 | Effective algaculture equipment |
| TWI638045B (en) * | 2016-10-11 | 2018-10-11 | 詮興開發科技股份有限公司 | Mass production method of microalgae |
| CN106566775B (en) * | 2016-10-21 | 2020-04-07 | 青岛科海生物有限公司 | Preparation method of high-activity haematococcus pluvialis cells |
| CN108323427B (en) * | 2017-08-08 | 2020-03-06 | 中国科学院成都生物研究所 | Method for improving duckweed starch yield by optimizing spectral composition |
| CN107548982B (en) * | 2017-08-08 | 2020-03-06 | 中国科学院成都生物研究所 | Method for improving total starch yield of duckweed by promoting dormancy formation |
| CN108083447B (en) * | 2018-01-12 | 2020-09-01 | 中国科学院成都生物研究所 | Method for promoting duckweed to rapidly purify micro-polluted surface water by utilizing high-quality light source |
| EP3686283A1 (en) * | 2019-01-22 | 2020-07-29 | Reliance Industries Limited | A method for enhancement of productivity in microalgae |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1181209C (en) * | 2003-02-24 | 2004-12-22 | 天津大学 | Algae with astaxanthin production and astaxanthin production by yeast mixed culture fermentation |
| CN201801523U (en) * | 2010-02-22 | 2011-04-20 | 中国科学院过程工程研究所 | Photo-biological reactor system for implementing light/dark alternated culture of microalgae cells |
| CN102337215A (en) * | 2011-10-20 | 2012-02-01 | 烟台华融生物科技有限公司 | Methods for culturing haematococcus pluvialis and producing astaxanthin |
-
2011
- 2011-10-20 CN CN2011103197681A patent/CN102337215A/en active Pending
-
2012
- 2012-08-20 CN CN201210295602.5A patent/CN102766578B/en not_active Expired - Fee Related
Non-Patent Citations (3)
| Title |
|---|
| 李颖逾: "雨生红球藻细胞转化及虾青素积累与营养因子关系研究", 《中国优秀硕士全文数据库基础科技辑》 * |
| 董庆霖: "利用雨生红球藻和红发夫酵母代谢过程中的协同效应提高虾青素产量", 《中国博士学位论文全文数据库工程科技I辑》 * |
| 黄永英等: "几种胁迫方式对雨生红球藻积累虾青素影响的初步研究", 《海洋科学集刊》 * |
Cited By (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102766578A (en) * | 2011-10-20 | 2012-11-07 | 烟台华融生物科技有限公司 | Cultivating and producing method for haematococcus pluvialis |
| CN102766578B (en) * | 2011-10-20 | 2014-04-09 | 烟台华融生物科技有限公司 | Cultivating and producing method for haematococcus pluvialis |
| CN102604836A (en) * | 2012-03-01 | 2012-07-25 | 武汉凯迪工程技术研究总院有限公司 | Production method and device for preventing chytrid pollution in haematococcus pluvialis |
| CN103114121A (en) * | 2013-01-31 | 2013-05-22 | 宁波大学 | Method for producing astaxanthin by haematococcus pluvialis |
| US20170107554A1 (en) * | 2014-04-03 | 2017-04-20 | Nippon Suisan Kaisha, Ltd. | Method for producing astaxanthin |
| WO2015151577A1 (en) * | 2014-04-03 | 2015-10-08 | 日本水産株式会社 | Method for producing astaxanthin |
| CN106133147A (en) * | 2014-04-03 | 2016-11-16 | 日本水产株式会社 | The production method of astaxanthin |
| JPWO2015151577A1 (en) * | 2014-04-03 | 2017-04-13 | 日本水産株式会社 | Production method of astaxanthin |
| CN105916993B (en) * | 2014-04-17 | 2019-09-27 | 高丽大学校产学协力团 | Method by improving the production of astaxanthin in haematococcus pluvialis in the Harber-Weiss reaction of high-temperature maturation spore inoculating and iron ion mediation |
| CN105916993A (en) * | 2014-04-17 | 2016-08-31 | 高丽大学校产学协力团 | Method for increasing production of astaxanthin in haematococcus pluvialis by mature spore inoculation and iron ion-mediated Harber-Weiss reaction at high temperature |
| CN104529852A (en) * | 2014-12-10 | 2015-04-22 | 青岛无为保温材料有限公司 | Method for extracting astaxanthin from haematococcus pluvialis |
| CN104531530A (en) * | 2014-12-10 | 2015-04-22 | 青岛无为保温材料有限公司 | Wall breaking method for haematococcus pluvialis |
| CN104856051A (en) * | 2015-04-10 | 2015-08-26 | 杭州鑫伟低碳技术研发有限公司 | Method for producing microcapsules of astaxanthin powder by utilizing haematococcus pluvialis |
| CN104886578A (en) * | 2015-06-15 | 2015-09-09 | 杭州鑫伟低碳技术研发有限公司 | Production device and production method for haematococcus pluvialis micro-capsule conidial powder |
| CN108350478B (en) * | 2015-09-11 | 2021-09-24 | 科隆大学 | A method for culturing Haematococcus organism for producing astaxanthin |
| CN108350478A (en) * | 2015-09-11 | 2018-07-31 | 科隆大学 | A method of cultivating the haematococcus biology for manufacturing astaxanthin |
| CN113862323A (en) * | 2015-09-11 | 2021-12-31 | 星际探索公司 | A method for culturing Haematococcus organism for producing astaxanthin |
| WO2018056160A1 (en) * | 2016-09-21 | 2018-03-29 | 日本水産株式会社 | Method for producing astaxanthin |
| CN109642246A (en) * | 2016-09-21 | 2019-04-16 | 日本水产株式会社 | The production method of astaxanthin |
| CN107099458A (en) * | 2017-04-06 | 2017-08-29 | 深圳市德和生物科技有限公司 | A kind of biological respinse kettle and the method for promoting haematococcus pluvialis propagation and redden |
| CN107354122A (en) * | 2017-09-18 | 2017-11-17 | 深圳市德和生物科技有限公司 | A kind of method for promoting haematococcus pluvialis growing multiplication and redden |
| CN110184168A (en) * | 2019-05-21 | 2019-08-30 | 荆楚理工学院 | A kind of simple astaxanthin incubator and the method using its culture astaxanthin |
| CN110064029A (en) * | 2019-05-24 | 2019-07-30 | 云南蓝钻生物科技股份有限公司 | A kind of pharmaceutical composition and preparation method thereof for treating gout |
| CN112842967A (en) * | 2021-03-05 | 2021-05-28 | 广州正广生物科技有限公司 | Anti-aging composition with function of removing free radicals and preparation method thereof |
| CN112998270A (en) * | 2021-04-12 | 2021-06-22 | 海南三元星生物科技股份有限公司 | Preparation method of astaxanthin microcapsules |
| CN113214998A (en) * | 2021-06-16 | 2021-08-06 | 江苏格局生物医药科技有限公司 | Preparation method of natural astaxanthin |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102766578A (en) | 2012-11-07 |
| CN102766578B (en) | 2014-04-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102337215A (en) | Methods for culturing haematococcus pluvialis and producing astaxanthin | |
| CN103459585B (en) | Process for production of microalgae, cyanobacteria and metabolites thereof | |
| CN103820325B (en) | Oocystis Borgei high-density cultivation method and frustule collection method | |
| CN108410939A (en) | A method of improving Determination of Astaxanthin in Haematococcus Pluvialis content | |
| CN209722114U (en) | A kind of salt algae culturing device | |
| CN107916211A (en) | Mass production method of microalgae | |
| CN101974600A (en) | Method for producing astaxanthin by haematococcus pluvialis induced by methyl jasmonate | |
| CN102524120A (en) | Big pool simulation culturing method of US Hippocampus kelloggi larvae | |
| KR101160069B1 (en) | Production method of functional rice with medicinal materials in mushrooms cordyceps militaris | |
| CN107326058A (en) | Use the method for Haematococcus pluvialis production astaxanthin | |
| CN102057888A (en) | Artificial culturing method and device for large water flea | |
| CN106520559B (en) | A kind of chlorella high efficiency light autotrophy cultural method | |
| CN106434817B (en) | Method for improving haematococcus pluvialis production of astaxanthin by using alkali pretreatment technology | |
| CN106399108A (en) | Simple high-efficiency haematococcus pluvialis nutritive cell culturing and harvesting method | |
| CN104480178B (en) | The method for coercing haematococcus pluvialis Rapid Accumulation astaxanthin | |
| CN104726321B (en) | A kind of racetrack bioreactor suitable for sunlight batch production | |
| CN102511450A (en) | Artificial domestication method for wild leeches | |
| CN106868085A (en) | A kind of method for promoting haematococcus pluvialis rapid conversion to accumulate astaxanthin | |
| CN106480155B (en) | Method suitable for promoting haematococcus pluvialis to produce astaxanthin under high-temperature condition | |
| CN101015277A (en) | Tissue culture method for chrysanthemum under LED condition | |
| CN102106285A (en) | Kishinouyea pond three-harvest raising technology | |
| CN106719255B (en) | A kind of prawn nursery pond and apply its prawn seed-rearing method | |
| RU2730670C2 (en) | Method of culturing type haematococcus for the production of astaxanthin | |
| CN102668966B (en) | Energy-saving seed-rearing technology of kelp | |
| CN110226541A (en) | A method of adjusting stichopus japonicus nursery water quality and control enemy planktonic organism breeding |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20120201 |