CN108323427B - Method for improving duckweed starch yield by optimizing spectral composition - Google Patents
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Abstract
The invention belongs to the field of aquatic energy plant culture, and provides a method for improving duckweed starch yield by optimizing spectral composition. The method can save cost, improve production efficiency, effectively increase duckweed starch yield, is not limited by water nutrition, and has universal applicability.
Description
Technical Field
The invention belongs to the field of aquatic energy plant culture, and particularly relates to a method for improving duckweed starch yield by optimizing spectral composition.
Background
Duckweed is the smallest flowering plant in the world and is usually grown floating on water. The whole duckweed plant comprises 37 plants of 5 genera, and has strong adaptability and wide distribution. In recent years, duckweed has received great attention as a novel energy plant. Under the optimal culture condition, the duckweed can double the biomass within 16-48 hours. Under specific culture conditions, the duckweed can rapidly accumulate starch, and the starch content can reach 45 percent at most.
Currently, methods for inducing duckweed starch accumulation include: oligotrophic treatment, hormonal treatment, treatment at high CO2Culturing under concentration condition and culturing with high illumination intensity. These methods all have a promoting effect on starch accumulation, but have the following limitations: (1) the oligotrophic treatment has high requirements on water quality and has weak applicability; (2) the operation of hormone treatment is complex, environmental pollution can be caused under the pilot-scale test condition, meanwhile, the cost is increased, and the economy is not good; (3) at high CO2Culturing under concentration condition firstly needs to build a relatively closed culture environment in order to maintain CO2Concentration, also needs to be paid attention to make up CO2The operation is more complex and the production cost is higher; (4) the cultivation with high illumination intensity can increase the light energy input, the power consumption is too high, and the economy is not good.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide a method for improving the yield of duckweed starch by optimizing the spectral composition so as to reduce the production cost while increasing the yield of the duckweed starch.
The method for improving the yield of the duckweed starch by optimizing the spectral composition, provided by the invention, comprises the steps of inoculating duckweed into a water body, culturing for 5-14 days under the irradiation condition of red light, blue light or red-blue-green mixed light, and harvesting the duckweed.
In the method, the photon flux density (PPFD) of red light, blue light or red-blue-green mixed light is 80-200 mu mol/m2Culturing duckweed under the illumination condition of/s.
In the method, the peak wavelength of red light is 660nm, the peak wavelength of blue light is 450nm, the peak wavelength of green light is 520nm, and the narrower the half-height peak widths of the red light, the blue light and the green light are, the better the improvement effect of the method on the yield of the duckweed starch is, preferably, the half-height peak width of the red light is not more than 15nm, the half-height peak width of the blue light is not more than 20nm, and the half-height peak width of the green light is not more than 15 nm.
In the above method, when the mixed red-blue-green light is used for illumination, the ratio of the light quantum flux density of the red light, the blue light and the green light in the mixed red-blue-green light is (3.5-4.5): (1.5-2.5): 0.5-1.5)
In the above method, duckweed is preferably cultured under irradiation with red light or red-blue-green mixed light, and more preferably under irradiation with red-blue-green mixed light.
In the method, the red light LED lamp is adopted to provide red light, the blue light LED lamp is adopted to provide blue light, and the red light LED lamp, the blue light LED lamp and the green light LED lamp are combined to provide red-blue-green mixed light.
In the method, in order to improve the yield of the duckweed starch to the maximum extent, the duckweed is preferably planted in the water body according to the coverage rate of 80-100%.
Compared with the prior art, the invention has the following beneficial effects:
1. the invention provides a method for improving duckweed starch yield by optimizing spectral composition, which can promote duckweed growth and starch accumulation by using a monochromatic red light or blue light LED lamp or a red-blue-green mixed light LED lamp as a light source, effectively improve the duckweed starch yield, and reduce the consumption of light energy in the duckweed culture process and save power resources because the light quality is optimized and unnecessary spectral composition is removed. Compared with the existing method for increasing the duckweed starch yield, the method provided by the invention can save cost, improve production efficiency and effectively increase the duckweed starch yield.
2. The method provided by the invention can be used for culturing the duckweed and the duckweed under the conditions of oligotrophism and eutrophication, the starch content and the dry matter accumulation speed of the duckweed and the duckweed can be improved, the starch yield is effectively improved, and the removal of N in a water body can be promoted by culturing the duckweed under the eutrophication condition. The method can effectively improve the yield of the duckweed starch without being limited by the water nutrition condition, and has universal applicability.
3. Compared with a white light LED lamp as a light source, the method can effectively reduce the electric energy consumption while improving the yield of the duckweed starch, and particularly has more obvious energy-saving advantage when a red-blue-green mixed light LED lamp and a red light LED lamp are adopted as the light source. The experiment shows that the light quantum flux density is 110 mu mol/m2Culturing duckweed under the illumination condition of/s, and adopting white light LEDCompared with the method using a lamp as a light source, the method of the invention adopts a red-blue-green mixed light LED lamp and a red light LED lamp as light sources, which can respectively reduce the electric energy consumption by 17.25% and 19.66%; also at a photon flux density of 110. mu. mol/m2Under the illumination condition of/s, the electricity consumption of starch production by using a white light LED lamp as a light source is 0.81W/g starch under the condition of rich nutrition, while the electricity consumption of starch production by using a blue light LED lamp, a red light LED lamp and a red-blue-green mixed light LED lamp as light sources is 0.18W/g starch, 0.13W/g starch and 0.09W/g starch respectively, so that the conversion efficiency from electric energy to effective biomass is higher.
Drawings
FIG. 1 is a graph comparing the dry matter accumulation rates of duckweed in comparative example 1 and examples 1-3.
FIG. 2 is a graph comparing the starch content of duckweed in comparative example 1 and examples 1-3.
FIG. 3 is a graph comparing the starch accumulation rates of duckweed in comparative example 1 and examples 1-3.
FIG. 4 is a graph comparing the irradiance of the light source and the energy conversion efficiency in comparative example 1 and examples 1 to 3.
FIG. 5 is a graph comparing the total nitrogen and total phosphorus removal rates in water in comparative example 1 and examples 1 to 3.
FIG. 6 is a graph comparing the dry matter accumulation rates of duckweed in comparative example 2 and examples 4-6.
FIG. 7 is a graph comparing the starch content of duckweed in comparative example 2 and examples 4-6.
FIG. 8 is a graph comparing the starch accumulation rates of duckweed in comparative example 2 and examples 4-6.
FIG. 9 is a graph comparing the dry matter accumulation rates of duckweed in comparative example 3 and examples 7-9.
FIG. 10 is a graph comparing the starch content of duckweed in comparative example 3 and examples 7-9.
FIG. 11 is a graph comparing the starch accumulation rates of duckweed in comparative example 3 and examples 7-9.
Detailed Description
The method for improving duckweed starch yield by optimizing spectral composition according to the present invention is further illustrated by the following examples and comparative examples.
In the following examples and comparative examples, the determination of duckweed starch content is described in Zhang L, Chen Q, Jin Y, et al, energy-saving direct ethanol production from vision reduction map of sweet spot to top high quality (VHG), Fuel processing technology 2010,91(12), 1845-1850. The specific determination method comprises the following steps: respectively crushing dried duckweed into powder, weighing 0.03-0.06 g of duckweed dry powder, placing the duckweed dry powder into a 250mL ground conical flask, adding 30mL of 6mol/L HCl solution and 100mL of distilled water, installing a condensing tube, and placing the mixture in a boiling water bath for refluxing for 2 hours. And (4) immediately cooling the mixture by using flowing water after the reflux is finished, and adding NaOH to adjust the pH value of the hydrolysate to 7 after the duckweed sample hydrolysate is cooled to room temperature. Then adding 20mL of 20 wt% lead acetate solution, shaking uniformly, standing for 10min, transferring to a 500mL volumetric flask, adding distilled water to a constant volume of 500mL, filtering, discarding the primary filtrate, collecting 5mL of filtrate, passing through a preactivated reverse phase C18 solid phase extraction column, discarding the primary 1-2 mL, collecting the subsequent 3-4 mL, and filtering with a 0.22 mu m water-based filter membrane. The glucose content of the filtrate was measured by HPLC, and the duckweed starch content was calculated from the starch content ═ glucose content/1.1.
The accumulation rate of duckweed starch is calculated according to the following formula:
Gstarch＝(Ct*Wt-C0*W0)/100/S/t(g/m2/d)
Gs＝GStarch*365*10000/1000/1000(t/hac/y)
In the above formula, CtThe content (%) of the duckweed starch in t days, C0The initial starch content (%) of duckweed, WtDry weight (g), W of duckweed harvested for t days0Is the initial starch dry weight (g), S is the inoculation area (m)2) And t is the incubation time (d).
The total nitrogen and total phosphorus concentration in the water body for culturing the duckweed is measured by a multifunctional water quality analyzer PhotoLab 6100(WTW, Germany), the used reagent is a matching reagent of Merck Corp (Germany), and the operation is carried out according to the instruction of the matching reagent.
In the following examples, the preparation of each solution was as follows:
the preparation method of the Hoagland culture solution comprises the following steps: (1) a, B, C, D, E, F kinds of mother liquor were prepared from distilled water according to the concentration of each reagent in the mother liquor formulation shown in the following table, wherein, when preparing the mother liquor A, Ca (NO) was first dissolved in 6N HCl solution3)2·4H2O、KNO3、KH2PO4Dissolving, and then adding distilled water to prepare target concentration; when preparing the mother liquor D, dissolving EDTA by 6N KOH solution, and then adding distilled water to prepare the target concentration. (2) Hoagland broth was prepared with distilled water according to the amounts of each mother liquor added per liter of Hoagland broth described in the table below, and the pH of the Hoagland broth was adjusted to 5.0 with HCl and NaOH. The concentration of the N element in the prepared Hoagland culture solution is 349.73mg/L, and the concentration of the P element is 154.89 mg/L.
1/5Hoagland culture solution preparation method: mixing a certain amount of Hoagland culture solution with distilled water with the volume 4 times of that of the Hoagland culture solution uniformly to obtain the compound.
The duckweed xj3 used in the following examples and comparative examples was collected from oolong village, xinjin county, city, Sichuan province, China.
The light source adopted in each proportion is a common white light LED lamp, and the light quality composition is as follows: blue/green/red (B/G/R) 37/45/28, which is the ratio of the photon flux density of blue, green and red light. In the following embodiments, red light and blue light are provided by using a red LED lamp and a blue LED lamp, respectively, and the red LED lamp, the blue LED lamp and the green LED lamp are combined into a red-blue-green mixed light LED lamp to provide red-blue-green mixed light. The peak wavelength of the red light is 660nm, the half-height peak width is 15nm, the peak wavelength of the blue light is 450nm, the half-height peak width is 20nm, the peak wavelength of the green light is 520nm, and the half-height peak width is 15 nm. When providing the red-blue-green mixed light, the red LED lamp, the blue LED lamp and the green LED lamp are combined to form the red-blue-green mixed light LED lamp with the light quantum flux density ratio of red/blue/green (R/B/G) ═ 4/2/1 according to the application requirements of the embodiment.
In the following comparative examples and comparative examples, the optical quantum flux density was 110. mu. mol/m2And culturing the duckweed under full illumination under the illumination condition of/s, wherein the light quantum flux density refers to the optical density of the corresponding light source on the surface of the water body inoculated with the duckweed.
Comparative example 1: ordinary white light LED lamp and rich nutrition condition culture
Inoculating azolla parvifolia xj3 into 1/5Hoagland nutrient solution of 250mL according to 100% coverage, wherein the total nitrogen concentration in the 1/5Hoagland nutrient solution is 70mg/L, the total phosphorus concentration is 30mg/L, a common white light LED lamp is adopted as a light source, and the light quantum flux density is 110 mu mol/m at 25 DEG according to the light quantum flux density2And (2) performing full-light culture under the illumination condition of/s, supplementing evaporated water to the height of the original liquid level by using distilled water every day, culturing for 5 days, harvesting the duckweed xj3, and simultaneously collecting 10mL of water sample for culturing the duckweed xj3 to store the water quality to be detected at 4 ℃.
Washing the harvested duckweed xj3 with distilled water for three times, placing the duckweed xj3 in a filter bag, removing free water by a spin dryer, drying the duckweed xj3 in an oven at 60 ℃ overnight to constant weight, weighing and recording, and calculating the dry matter accumulation speed of the duckweed xj 3. And grinding the dried sample into powder, weighing a proper amount of the powder, measuring the glucose content by using an HPLC method after acidolysis, and calculating the starch content. The result shows that the average dry matter accumulation speed of the duckweed xj 35 days is 4.21g/m2D, starch content 3.65%, starch accumulation rate 0.77t/hac/y (ton/hectare/year). The water quality measurement result shows that the azolla xiaogen xj3 grows for 5 days, and the removal rate of total nitrogen and total phosphorus in the culture solution is 42.34% and 24.09% respectively.
Example 1: blue light LED lamp + eutrophication condition culture
Inoculating azolla parvifolia xj3 into 1/5Hoagland nutrient solution of 250mL according to 100% coverage, wherein the total nitrogen concentration in the 1/5Hoagland nutrient solution is 70mg/L, the total phosphorus concentration is 30mg/L, a blue light LED lamp is adopted as a light source, and the light quantum flux density is 110 mu mol/m at 25 DEG according to the light quantum flux density2Performing full-light culture under the illumination condition of/s, supplementing evaporated water with distilled water every day to the height of the original liquid level, culturing for 5 days, harvesting the duckweed xj3, and collecting and culturing the duckweed10mL of water sample of xj3 is used for storing the water quality to be detected at 4 ℃.
The dry matter accumulation rate and the starch content of the duckweed xj3 harvested in this example were determined and calculated using the same experimental conditions as in comparative example 1, and the results showed that the average dry matter accumulation rate of the duckweed xj 35 for days was 5.00g/m2And d, the starch content is 13.9 percent, and the starch accumulation speed is 4.02 t/hac/y. The water quality measurement result shows that the azolla xiaogen xj3 grows for 5 days, and the removal rate of total nitrogen and total phosphorus in the culture solution is 39.12% and 25.72% respectively.
Example 2: red light LED lamp and nutrient enrichment condition culture
Inoculating azolla parvifolia xj3 into 1/5Hoagland nutrient solution of 250mL according to 100% coverage, wherein the total nitrogen concentration in the 1/5Hoagland nutrient solution is 70mg/L, the total phosphorus concentration is 30mg/L, a red light LED lamp is adopted as a light source, and the light quantum flux density is 110 mu mol/m at 25 DEG C2And (2) performing full-light culture under the illumination condition of/s, supplementing evaporated water to the height of the original liquid level by using distilled water every day, culturing for 5 days, harvesting the duckweed xj3, and simultaneously collecting 10mL of water sample for culturing the duckweed xj3 to store the water quality to be detected at 4 ℃.
The dry matter accumulation rate and the starch content of the duckweed xj3 harvested in this example were determined and calculated using the same experimental conditions as in comparative example 1, and the results showed that the average dry matter accumulation rate of the duckweed xj 35 for days was 7.11g/m2And d, the starch content is 10.5 percent, and the starch accumulation speed is 3.78 t/hac/y. The water quality measurement result shows that the azolla xiaogen xj3 grows for 5 days, and the removal rate of total nitrogen and total phosphorus in the culture solution is 45.41% and 25.54% respectively.
Example 3: R/B/G4/2/1 mixed light LED lamp and eutrophic condition culture
Inoculating azolla parvifolia xj3 with 100% coverage in 1/5Hoagland nutrient solution of 250mL, wherein the total nitrogen concentration in the 1/5Hoagland nutrient solution is 70mg/L, the total phosphorus concentration is 30mg/L, a red-blue-green mixed light LED lamp with the light quantum flux density ratio of red light, blue light and green light being 4:2:1 is adopted as a light source, and the light quantum flux density is 110 mu mol/m at 25 DEG C2The culture is performed under full illumination condition, and distilled water is used for supplementing evaporated water every dayAnd culturing for 5 days until the height of the original liquid level is reached, harvesting the duckweed xj3, and simultaneously collecting 10mL of water sample for culturing the duckweed xj3 to store the water quality to be detected at 4 ℃.
The dry matter accumulation rate and the starch content of the duckweed xj3 harvested in this example were determined and calculated using the same experimental conditions as in comparative example 1, and the results showed that the average dry matter accumulation rate of the duckweed xj 35 for days was 7.89g/m2And d, the starch content is 14.8 percent, and the starch accumulation speed is 5.86 t/hac/y. The water quality measurement result shows that the azolla xiaogen xj3 grows for 5 days, and the removal rate of total nitrogen and total phosphorus in the culture solution is 54.08 percent and 25.72 percent respectively.
As can be seen from FIGS. 1 to 3, the average dry matter accumulation rate of the duckweed xj3 reaches 5.0 to 7.89g/m under the condition of rich nutrition by adopting blue light, red light and red-blue-green mixed light for irradiation and culture for 5 days2The starch content is 10.5-13.94%, and the average dry matter accumulation speed of the duckweed xj3 is 4.21g/m after the culture is performed for 5 days by adopting white light irradiation2And d, the starch content is only 3.65 percent, and the starch yield is increased from 0.77t/hac/y to 5.86 t/hac/y. The method can simultaneously improve the dry matter accumulation speed and the starch content of the duckweed and effectively improve the starch yield of the duckweed.
As can be seen from FIG. 4, under the condition of the same light quantum flux density, the irradiance of the red light and the red-blue-green mixed light adopted by the invention is lower than that of the common white light, and meanwhile, the energy conversion efficiency of the method for culturing the azolla parvifolia xj3 under the condition of rich nutrition by adopting the red light and the red-blue-green mixed light irradiation is obviously higher than that of the method for culturing under the condition of rich nutrition by adopting the common white light irradiation. The power consumption of starch produced by using a white light LED lamp as a light source is 0.81W/g starch, while the power consumption of starch produced by using a blue light LED lamp, a red light LED lamp and a red-blue-green mixed light LED lamp as light sources is 0.18W/g starch, 0.13W/g starch and 0.09W/g starch respectively, so that the conversion efficiency from electric energy to effective biomass is obviously higher.
As can be seen from FIG. 5, compared with the case of using the common white light for illumination, when the method of the present invention uses the red light and the red-blue-green mixed light for illumination to culture the duckweed xj3 with few roots under the condition of eutrophication, the total nitrogen removal rate in the water body is significantly improved under the condition that the total phosphorus removal rate in the water body is basically unchanged.
Comparative example 2: ordinary white light LED lamp and oligotrophic condition culture
Inoculating Spirodela polyrhiza xj3 in 250mL tap water at 100% coverage, using common white light LED lamp as light source, and measuring the luminous flux density at 25 deg.C to 110 μmol/m2And (3) performing full-light culture under the illumination condition of/s, supplementing evaporated water to the height of the original liquid level with distilled water every day, culturing for 5 days, and harvesting the duckweed xj 3.
The dry matter accumulation rate and the starch content of the duckweed xj3 harvested in the comparative example were determined and calculated by using the same experimental conditions as those of the comparative example 1, and the result shows that the average dry matter accumulation rate of the duckweed xj 35 for days is 5.42g/m2And d, the starch content is 31.52%, and the starch accumulation speed is 8.94 t/hac/y.
Example 4: blue light LED lamp + oligotrophic condition culture
Inoculating azolla xiaogen xj3 in 250mL tap water according to 100% coverage, adopting a blue light LED lamp as a light source, and obtaining a luminous flux density of 110 mu mol/m at 25 DEG according to the light quantum flux density2And (3) performing full-light culture under the illumination condition of/s, supplementing evaporated water to the height of the original liquid level with distilled water every day, culturing for 5 days, and harvesting the duckweed xj 3.
The dry matter accumulation rate and the starch content of the duckweed xj3 harvested in this example were determined and calculated using the same experimental conditions as in comparative example 1, and the results showed that the average dry matter accumulation rate of the duckweed xj 35 for days was 6.99g/m2And d, the starch content is 47.23%, and the starch accumulation speed is 16.39 t/hac/y.
Example 5: red light LED lamp and oligotrophic condition culture
Inoculating the duckweed xj3 into 250mL of tap water according to 100% coverage, adopting a red light LED lamp as a light source, and obtaining the duckweed xj3 with the luminous quantum flux density of 110 mu mol/m at 25 DEG C2Culturing under full illumination condition of/s, supplementing evaporated water with distilled water to the height of original liquid level every day, culturing for 5 days, and harvesting few rootsDuckweed xj 3.
The dry matter accumulation rate and the starch content of the duckweed xj3 harvested in this example were determined and calculated using the same experimental conditions as in comparative example 1, and the results showed that the average dry matter accumulation rate of the duckweed xj 35 for days was 7.26g/m2And d, the starch content is 53.28%, and the starch accumulation speed is 19.20 t/hac/y.
Example 6: cultivation under conditions of mixed light LED lamp and oligotrophic culture with R/B/G:4/2/1
Inoculating the duckweed xj3 with 100% coverage in 250mL of tap water, adopting a red-blue-green mixed light LED lamp with the light quantum flux density ratio of red light, blue light and green light of 4:2:1 as a light source, and adopting the light quantum flux density of 110 mu mol/m at 25 DEG C2And (3) performing full-light culture under the illumination condition of/s, supplementing evaporated water to the height of the original liquid level with distilled water every day, culturing for 5 days, and harvesting the duckweed xj 3.
The dry matter accumulation rate and the starch content of the duckweed xj3 harvested in this example were determined and calculated using the same experimental conditions as in comparative example 1, and the results showed that the average dry matter accumulation rate of the duckweed xj 35 for days was 8.40g/m2And d, the starch content is 61.34 percent, and the starch accumulation speed is 24.79 t/hac/y.
As can be seen from FIGS. 6 to 8, the average dry matter accumulation rate of the duckweed xj3 reaches 6.99 to 8.4g/m under oligotrophic conditions, when the duckweed is cultured for 5 days by irradiation with blue light, red light and red-blue-green mixed light2The starch content is 47.23% -61.34%, the average dry matter accumulation speed of the duckweed xj3 is 5.42g/m after the culture is performed for 5 days by adopting white light irradiation2The starch content is only 31.52%, and the yield of the starch is increased from 8.94t/hac/y to 24.7 t/hac/y. The method can simultaneously improve the dry matter accumulation speed and the starch content of the duckweed and effectively improve the starch yield of the duckweed.
Comparative example 3: ordinary white light LED lamp and rich nutrition condition culture
The duckweed is inoculated into 1/5Hoagland nutrient solution with the concentration of 100 percent in 250mL, the total nitrogen concentration and the total phosphorus concentration in the nutrient solution with the concentration of 1/5Hoagland are respectively 70mg/L and 30mg/L, a common white light LED lamp is adopted as a light source, and the temperature is 25 DEG CAccording to the light quantum flux density of 110 mu mol/m2And (3) performing full-light culture under the illumination condition of/s, supplementing evaporated water to the height of the original liquid level with distilled water every day, culturing for 5 days, and harvesting the duckweed.
The dry matter accumulation rate and starch content of the harvested duckweed of this example were determined and calculated using the same experimental conditions as in comparative example 1, and the results showed that the balance average dry matter accumulation rate of duckweed 5 was 5.00g/m2And d, the content of the duckweed starch is 4.18 percent, and the accumulation rate of the duckweed starch is 0.92 t/hac/y.
Example 7: blue light LED lamp + eutrophication condition culture
Inoculating the duckweed into 250mL of 1/5Hoagland nutrient solution according to 100% coverage, wherein the total nitrogen concentration and the total phosphorus concentration in the 1/5Hoagland nutrient solution are respectively 70mg/L and 30mg/L, a blue light LED lamp is adopted as a light source, and the light quantum flux density is 110 mu mol/m at 25 DEG C2And (3) performing full-light culture under the illumination condition of/s, supplementing evaporated water to the height of the original liquid level with distilled water every day, culturing for 5 days, and harvesting the duckweed.
The dry matter accumulation rate and starch content of the harvested duckweed of this example were determined and calculated using the same experimental conditions as in comparative example 1, and the results showed that the balance average dry matter accumulation rate of duckweed 5 was 7.81g/m2And d, the content of the duckweed starch is 17.72 percent, and the accumulation rate of the duckweed starch is 7.08 t/hac/y.
Example 8: red light LED lamp and nutrient enrichment condition culture
Inoculating the duckweed into 250mL of 1/5Hoagland nutrient solution according to 100% coverage, wherein the total nitrogen concentration and the total phosphorus concentration in the 1/5Hoagland nutrient solution are respectively 70mg/L and 30mg/L, a common red light LED lamp is adopted as a light source, and the light quantum flux density is 110 mu mol/m at 25 DEG C2And (3) performing full-light culture under the illumination condition of/s, supplementing evaporated water to the height of the original liquid level with distilled water every day, culturing for 5 days, and harvesting the duckweed.
The dry matter accumulation rate and starch content of the harvested duckweed of this example were determined and calculated using the same experimental conditions as in comparative example 1, and the results showed that the balance average dry matter accumulation rate of duckweed 5 was 8.51g/m2D, the content of duckweed starch is 22.46 percent, and duckweedThe starch accumulation rate is 9.53 t/hac/y.
Example 9: R/B/G4/2/1 mixed light LED lamp and eutrophic condition culture
Inoculating duckweed into 1/5Hoagland nutrient solution with a concentration of 100% in 250mL, wherein the total nitrogen concentration in the 1/5Hoagland nutrient solution is 70mg/L, the total phosphorus concentration is 30mg/L, a red-blue-green mixed light LED lamp with a light quantum flux density ratio of red light, blue light and green light of 4:2:1 is used as a light source, and the light quantum flux density is 110 mu mol/m at 25 DEG C2And (3) performing full-light culture under the illumination condition of/s, supplementing evaporated water to the height of the original liquid level with distilled water every day, culturing for 5 days, and harvesting the duckweed.
The dry matter accumulation rate and starch content of the harvested duckweed of this example were determined and calculated using the same experimental conditions as in comparative example 1, and the results showed that the balance average dry matter accumulation rate of duckweed 5 was 9.39g/m2And d, the content of the duckweed starch is 21.94%, and the accumulation rate of the duckweed starch is 10.14 t/hac/y.
As can be seen from FIGS. 9 to 11, the average dry matter accumulation rate of the duckweed reaches 7.81 to 9.39g/m after the culture is performed for 5 days under the condition of rich nutrition by adopting blue light, red light and red-blue-green mixed light for irradiation2The starch content is 17.72-22.46%, and the average dry matter accumulation speed of the duckweed is 5.00g/m after the duckweed is cultured for 5 days by adopting white light irradiation2And d, the content of the duckweed starch is only 4.18 percent, and the yield of the duckweed starch is increased from 0.92t/hac/y to 10.14t/hac/y, which shows that the method disclosed by the invention is not limited to the duckweed with few roots and can also effectively improve the yield of the duckweed starch.
Claims (5)
1. A method for improving duckweed starch yield by optimizing spectral composition is characterized in that duckweed is inoculated in a water body, and the light quantum flux density is 80-200 mu mol/m2Culturing for 5-14 days under the irradiation condition of red-blue-green mixed light per second, and harvesting duckweed;
the ratio of the light quantum flux density of the red light, the blue light and the green light in the red-blue-green mixed light is (3.5-4.5): (1.5-2.5): 0.5-1.5), and the duckweed is duckweed with few roots or duckweed.
2. The method for improving duckweed starch yield by optimizing spectral composition as claimed in claim 1, wherein the peak wavelength of red light is 660nm, the peak wavelength of blue light is 450nm and the peak wavelength of green light is 520 nm.
3. The method for improving duckweed starch yield by optimizing spectral composition as claimed in claim 2, wherein the peak width at half maximum of red light is not more than 15nm, the peak width at half maximum of blue light is not more than 20nm, and the peak width at half maximum of green light is not more than 15 nm.
4. The method for improving duckweed starch production by optimizing spectral composition as claimed in claim 1, wherein red LED lamps, blue LED lamps and green LED lamps are combined to provide a red-blue-green mixed light.
5. The method for improving duckweed starch yield by optimizing spectral composition as claimed in claim 1, wherein duckweed is planted in the water body at a coverage rate of 80% to 100%.
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