CN108323427A - A method of improving duckweed starch yield by optimizing spectral composition - Google Patents
A method of improving duckweed starch yield by optimizing spectral composition Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/04—Electric or magnetic or acoustic treatment of plants for promoting growth
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/14—Measures for saving energy, e.g. in green houses
Abstract
The invention belongs to the invention belongs to aquatic energy-source plant culture fields, provide a kind of method improving duckweed starch yield by optimizing spectral composition, duckweed is inoculated in water body by this method, is cultivated 5~14 days under the irradiation condition of feux rouges, blue light or red bluish-green mixed light, harvests duckweed.This method can improve production efficiency while cost-effective, be effectively increased duckweed starch yield, and not limited by water nutrition situation, have general applicability.
Description
Technical field
It is the invention belongs to aquatic energy-source plant culture field, more particularly to a kind of to improve duckweed shallow lake by optimizing spectral composition
The method of powder yield.
Background technology
Duckweed is the flowering plant of minimum in the world, usually swims on the water surface and is grown.Entire plant and of its applications packet
5 37 kinds of categories are included, it is adaptable, it is distributed more widely.In recent years, duckweed receives people's as a kind of novel energy plant
Greatly concern.Under optimal condition of culture, duckweed can realize that biomass is double in 16~48 hours.And in specific training
Under the conditions of supporting, duckweed can carry out the Rapid Accumulation of starch, and content of starch reaches as high as 45%.
Currently, the method for induction duckweed starch accumulation has：Oligotrophic processing, HORMONE TREATMENT, in high CO2It is trained under concentration conditions
Support and use high intensity of illumination culture.These methods have facilitation to starch accumulation, but there are following limitations：(1)
Oligotrophic processing is very high to water quality requirement, and applicability is not strong；(2) operation of HORMONE TREATMENT is complex, can under pilot plant conditions
Environmental pollution can be caused, while can also increase cost, economy is bad；(3) in high CO2Under concentration conditions culture firstly the need of
A relatively closed culture environment is built, in order to keep CO2Concentration, it is also necessary to pay attention to supplement CO2, operation is complex, production
Cost is higher；(4) luminous energy input can be increased using high intensity of illumination culture, power consumption is excessively high, and economy is also bad.
Invention content
It is an object of the invention to overcome the deficiencies of the prior art and provide one kind improving duckweed shallow lake by optimizing spectral composition
The method of powder yield, to reduce production cost while increasing duckweed starch yield.
The method provided by the invention for improving duckweed starch yield by optimizing spectral composition, is that duckweed is inoculated in water body
In, it is cultivated 5~14 days under the irradiation condition of feux rouges, blue light or red-blue-green mixed light, harvests duckweed.
In the above method, the photon hypothesis (PPFD) according to feux rouges, blue light or red-blue-green mixed light is 80
~200 μm of ol/m2The illumination condition culture duckweed of/s.
In the above method, the peak wavelength of feux rouges is 660nm, and the peak wavelength of blue light is 450nm, the peak wavelength of green light
Peak width at half height for 520nm, feux rouges, blue light and green light is narrower, improvement effect of the method for the invention to duckweed starch yield
It is better, it is preferable that the peak width at half height of feux rouges is no more than 15nm, and the peak width at half height of blue light is no more than 20nm, the peak width at half height of green light
No more than 15nm.
In the above method, using red-blue-green mixed light irradiation when, feux rouges, blue light in red-blue-green mixed light with it is green
The ratio between photon hypothesis of light is (3.5~4.5):(1.5~2.5):(0.5~1.5)
In the above method, duckweed preferably is trained under the irradiation condition of feux rouges or red-blue-green mixed light, more preferably place
Case is to train duckweed under the irradiation condition of red-blue-green mixed light.
In the above method, feux rouges is provided using red LED lamp, blue light is provided using blue LED lamp, by red LED lamp, indigo plant
Light LED light closes with green light LED lamp group and provides red-blue-green mixed light.
In the above method, in order to improve duckweed starch yield to the maximum extent, preferably according to 80%~100% covering
Rate is by duckweed kind in water body.
Compared with prior art, the invention has the advantages that：
1. the method that the present invention provides a kind of to improve duckweed starch yield by optimizing spectral composition, this method pass through list
Color feux rouges or blue LED lamp either red-blue-green mixed light LED light promote duckweed growth and starch accumulation simultaneously as light source,
Duckweed starch yield is can effectively improve, simultaneously because optimizing light quality, removes unnecessary spectral composition, duckweed training can be reduced
The consumption of luminous energy during supporting, saves electric power resource.Compared with the existing method for increasing duckweed starch yield, method of the invention
Production efficiency can be improved while cost-effective, be effectively increased duckweed starch yield.
2. the method for the invention cultivates few root duckweed and duckweed under the conditions of oligotrophic and eutrophy, them can be improved
Content of starch and dry matter accumulation speed, the starch yield effectively improved, under the conditions of eutrophy cultivate duckweed can also promote
The removal of N in water body.Illustrate that the method for the present invention can effectively improve the starch yield of duckweed without being limited by water nutrition situation
System has general applicability.
3. compared with using white LED lamp as light source, the method for the invention is while improving duckweed starch yield
Power consumption can be effectively reduced, when especially with red-blue-green mixed light LED light and red LED lamp as light source, is had more
Apparent power savings advantages.Experiments have shown that being 110 μm of ol/m in photon hypothesis2Duckweed is cultivated under the illumination condition of/s, with
It is compared as light source using white LED lamp, the method for the invention is made using red-blue-green mixed light LED light and red LED lamp
It can reduce by 17.25% and 19.66% power consumption respectively for light source；It is equally 110 μm of ol/m in photon hypothesis2/s
Illumination condition under, in eutrophy condition, using white LED lamp as light source produce starch electric quantity consumption be 0.81 watt/g shallow lake
Powder, and the method for the invention is used to be produced using blue LED lamp, red LED lamp and red-blue-green mixed light LED light as light source
The electric quantity consumption of starch is respectively then 0.18 watt/g starch, 0.13 watt/g starch and 0.09 watt/g starch, is had higher from electricity
The transformation efficiency of effective biomass can be arrived.
Description of the drawings
Fig. 1 is the dry substance accumulation speed comparison diagram of duckweed in comparative example 1 and Examples 1 to 3.
Fig. 2 is the content of starch comparison diagram of duckweed in comparative example 1 and Examples 1 to 3.
Fig. 3 is the starch accumulation velocity contrast figure of duckweed in comparative example 1 and Examples 1 to 3.
Fig. 4 is light irradiation degree and energy conversion efficiency comparison diagram in comparative example 1 and Examples 1 to 3.
Fig. 5 is in comparative example 1 and Examples 1 to 3 to total nitrogen in water body and total tp removal rate comparison diagram.
Fig. 6 is the dry substance accumulation speed comparison diagram of duckweed in comparative example 2 and embodiment 4~6.
Fig. 7 is the content of starch comparison diagram of duckweed in comparative example 2 and embodiment 4~6.
Fig. 8 is the starch accumulation velocity contrast figure of duckweed in comparative example 2 and embodiment 4~6.
Fig. 9 is the dry substance accumulation speed comparison diagram of duckweed in comparative example 3 and embodiment 7~9.
Figure 10 is the content of starch comparison diagram of duckweed in comparative example 3 and embodiment 7~9.
Figure 11 is the starch accumulation velocity contrast figure of duckweed in comparative example 3 and embodiment 7~9.
Specific implementation mode
Below by embodiment and comparative example duckweed starch yield is improved by optimizing spectral composition to of the present invention
Method is described further.
In following each embodiments and comparative example, the assay method of duckweed content of starch can be found in Zhang L., Chen Q.,
Jin Y.,et al.Energy-saving direct ethanol production from viscosity reduction
mash of sweet potato at very high gravity(VHG).Fuel Processing
Technology.2010,91(12):1845-1850.Specifically assay method is：Dry duckweed is ground into powder respectively, is claimed
It takes 0.03~0.06g duckweed dry powder to be placed in 250mL ground conical flasks, HCl solution and the 100mL distillations of 30mL6mol/L is added
Water loads onto condenser pipe, sets the 2h that flows back in boiling water bath.Reflux finishes, and immediately with flowing water cooling, waits for duckweed sample hydrolysis liquid cooling but
To room temperature, it is 7 that NaOH, which is added, and adjusts the pH value of hydrolyzate.Then 20mL 20wt% acetic acid lead solutions are added, are placed after shaking up
10min is transferred in 500mL volumetric flasks, and distilled water is added to be settled to 500mL, and filtering discards primary filtrate, and it is excessively pre- to collect 5mL filtrates
Activated reverse phase C18 solid phase extraction columns, discard 1 initial~2mL, collect subsequent 3~4mL, then with 0.22 μm of water
It is membrane filtration.Glucose content in filtrate is measured using HPLC, according to content of starch=glucose content/1.1, calculates duckweed
Content of starch.
Duckweed starch accumulation speed is calculated as follows：
GStarch=(Ct*Wt-C0*W0)/100/S/t(g/m2/d)
Gs=GStarch*365*10000/1000/1000(t/hac/y)
In above formula, CtFor t days duckweed content of starch (%), C0For duckweed initial starch content (%), WtIt is floating for t days harvests
Duckweed dry weight (g), W0For initial starch dry weight (g), S is vaccination area (m2), t is incubation time (d).
The total nitrogen and total phosphorus concentration cultivated in the water body of duckweed pass through Multifunctional water analyzer PhotoLab 6100
(WTW, Germany) measures, and agents useful for same is the matched reagent of Merck ＆ Co., Inc. (Merck Corp, Germany), according to saying for matched reagent
Bright book is operated.
In following each embodiments, the preparation method of various solution is as follows：
The preparation method of Hoagland culture solutions is as follows：(1) with distilled water according to respectively being tried in the mother liquor formula described in following table
The concentration of agent prepares six kinds of mother liquors of A, B, C, D, E, F, wherein when preparing mother liquor A, first uses the HCl solution of 6N by Ca (NO3)2·
4H2O、KNO3、KH2PO4Then dissolving is added distilled water and is configured to aimed concn；When preparing mother liquor D, the KOH solution of 6N is first used
EDTA is dissolved, distilled water is then added and is configured to aimed concn.(2) according in every liter of Hoagland culture solution described in following table
The additive amount of various mother liquors prepares Hoagland culture solutions with distilled water, and Hoagland culture solutions are adjusted by HCl and NaOH
PH value is 5.0.In prepared Hoagland culture solutions, a concentration of 349.73mg/L of N element, P element it is a concentration of
154.89mg/L。
The preparation method of 1/5 concentration Hoagland culture solutions：By a certain amount of Hoagland culture solutions and 4 times of its volumes
Distilled water be uniformly mixed to obtain the final product.
The few root duckweed xj3 used in following embodiment and comparative examples is acquired from Sichuan Province China province Chengdu Xinjin County oolong
Village.
For common white LED lamp, light quality group becomes the light source used in following each comparative examples：Blue light/green/red
(B/G/R)=37/45/28, which is the ratio between the photon hypothesis of blue and green light and feux rouges.In following each embodiments,
Red LED lamp, blue LED lamp offer feux rouges and blue light is respectively adopted, by red LED lamp, blue LED lamp and green light LED lamp group
It synthesizes red-blue-green mixed light LED light and red-blue-green mixed light is provided.The peak wavelength of the feux rouges is 660nm, peak width at half height
For 15nm, the peak wavelength of blue light is 450nm, peak width at half height 20nm, and the peak wavelength of green light is 520nm, peak width at half height is
15nm.When red-blue-green mixed light is provided, application demand according to the embodiment, by red LED lamp, blue LED lamp and green light LED
It is feux rouges/blue light/green light (R/B/G)=4/2/1 that lamp group, which closes and to form the photon hypothesis ratio of feux rouges, blue light and green light,
Red-blue-green mixed light LED light.
It is 110 μm of ol/m according to photon hypothesis in following each comparative examples and embodiment2The full light of illumination condition of/s
According to culture duckweed, photon hypothesis therein each means that corresponding light source is close in the light for the water surface for being vaccinated with duckweed
Degree.
Comparative example 1：Common white LED lamp+eutrophy CMC model
Few root duckweed xj3 is inoculated in by 100% coverage in the 1/5Hoagland nutrient solutions of 250mL, 1/
Total nitrogen concentration in 5Hoagland nutrient solutions is 70mg/L, total phosphorus concentration 30mg/L, using common white LED lamp as light
Source, 25 DEG C according to photon hypothesis be 110 μm of ol/m2The illumination condition full exposure culture of/s, is mended with distilled water daily
The water evaporated is filled to former liquid level, is cultivated 5 days, is harvested few root duckweed xj3, while acquiring the water of the few root duckweed xj3 of culture
Sample 10mL preserves water quality to be measured at 4 DEG C.
After few root duckweed xj3 of harvest distilled water flushings three times, it is placed in filter bag and sloughs Free water through dryer, in
At 60 DEG C, drying to constant weight overnight in baking oven, and record of weighing, and calculates few root duckweed xj3 dry substance accumulation speeds.After dry
Sample mill powdering and claim in right amount after acidolysis with HPLC methods survey glucose content, calculate content of starch.The results show that few root
5 days average dry substance accumulation speeds of duckweed xj3 are 4.21g/m2/ d, content of starch 3.65%, starch accumulation speed are
0.77t/hac/y (ton/hectare/year).Water-quality determination the result shows that few root duckweed xj3 is grown 5 days, in culture solution total nitrogen and
The removal rate of total phosphorus is respectively 42.34% and 24.09%.
Embodiment 1：Blue LED lamp+eutrophy CMC model
Few root duckweed xj3 is inoculated in by 100% coverage in the 1/5Hoagland nutrient solutions of 250mL, 1/
Total nitrogen concentration in 5Hoagland nutrient solutions is 70mg/L, total phosphorus concentration 30mg/L, using blue LED lamp as light source,
25 DEG C are 110 μm of ol/m according to photon hypothesis2The illumination condition full exposure culture of/s is supplemented with distilled water evaporate daily
The water fallen is cultivated 5 days to former liquid level, harvests few root duckweed xj3, while acquiring the water sample 10mL of the few root duckweed xj3 of culture
Water quality to be measured is preserved at 4 DEG C.
The dry of the few root duckweed xj3 harvested in the present embodiment is measured and calculated using experiment condition identical with comparative example 1
Substance accumulating rate and content of starch, the results show that 5 days average dry substance accumulation speeds of few root duckweed xj3 are 5.00g/
m2/ d, content of starch 13.9%, starch accumulation speed are 4.02t/hac/y.Water-quality determination is the result shows that few root duckweed xj3 lifes
5 days long, the removal rate to total nitrogen and total phosphorus in culture solution is respectively 39.12% and 25.72%.
Embodiment 2：Red LED lamp+eutrophy CMC model
Few root duckweed xj3 is inoculated in by 100% coverage in the 1/5Hoagland nutrient solutions of 250mL, 1/
Total nitrogen concentration in 5Hoagland nutrient solutions is 70mg/L, total phosphorus concentration 30mg/L, using red LED lamp as light source,
25 DEG C are 110 μm of ol/m according to photon hypothesis2The illumination condition full exposure culture of/s is supplemented with distilled water evaporate daily
The water fallen is cultivated 5 days to former liquid level, harvests few root duckweed xj3, while acquiring the water sample 10mL of the few root duckweed xj3 of culture
Water quality to be measured is preserved at 4 DEG C.
The dry of the few root duckweed xj3 harvested in the present embodiment is measured and calculated using experiment condition identical with comparative example 1
Substance accumulating rate and content of starch, the results show that 5 days average dry substance accumulation speeds of few root duckweed xj3 are 7.11g/
m2/ d, content of starch 10.5%, starch accumulation speed are 3.78t/hac/y.Water-quality determination is the result shows that few root duckweed xj3 lifes
5 days long, the removal rate to total nitrogen and total phosphorus in culture solution is respectively 45.41% and 25.54%.
Embodiment 3：R/B/G:4/2/1 mixed light LED light+eutrophy CMC model
Few root duckweed xj3 is inoculated in by 100% coverage in the 1/5Hoagland nutrient solutions of 250mL, 1/
Total nitrogen concentration in 5Hoagland nutrient solutions is 70mg/L, total phosphorus concentration 30mg/L, using the light of feux rouges, blue light and green light
Quantum flux density ratio is 4:2:1 red-blue-green mixed light LED light is according to photon hypothesis as light source, at 25 DEG C
110μmol/m2The illumination condition full exposure culture of/s, the water evaporated daily with distilled water supplement cultivate 5 to former liquid level
It, harvests few root duckweed xj3, while the water sample 10mL for acquiring the few root duckweed xj3 of culture preserves water quality to be measured at 4 DEG C.
The dry of the few root duckweed xj3 harvested in the present embodiment is measured and calculated using experiment condition identical with comparative example 1
Substance accumulating rate and content of starch, the results show that 5 days average dry substance accumulation speeds of few root duckweed xj3 are 7.89g/
m2/ d, content of starch 14.8%, starch accumulation speed are 5.86t/hac/y.Water-quality determination is the result shows that few root duckweed xj3 lifes
5 days long, the removal rate to total nitrogen and total phosphorus in culture solution is respectively 54.08% and 25.72%.
By Fig. 1~3 it is found that using blue light, feux rouges and red-blue-green mixed light irradiation culture 5 under the conditions of eutrophy
It, few root duckweed xj3 dry substance accumulation speeds that are averaged have reached 5.0~7.89g/m2/ d, content of starch be 10.5%~
13.94%, and white light culture 5 days is used, few root duckweed xj3 is averaged dry substance accumulation speed as 4.21g/m2/ d, starch
Content is only 3.65%, and starch yield has increased to 5.86t/hac/y by 0.77t/hac/y.Illustrate the method for the invention energy
The dry substance accumulation speed and content of starch for improving duckweed simultaneously, effectively improve the starch yield of duckweed.
As shown in Figure 4, under conditions of identical photon hypothesis, the feux rouges and red-blue-green that the present invention uses are mixed
The irradiation level of closing light is less than common white light, meanwhile, the present invention is radiated at eutrophy condition using feux rouges and red-blue-green mixed light
The energy conversion efficiency of the lower few root duckweed xj3 of culture is apparently higher than when being cultivated under the conditions of eutrophy using common white light
Energy conversion efficiency.The electric quantity consumption that starch is produced using white LED lamp as light source is 0.81 watt/g starch, and with blue-ray LED
The electric quantity consumption that lamp, red LED lamp and red-blue-green mixed light LED light produce starch as light source is respectively then that 0.18 watt/g forms sediment
Powder, 0.13 watt/g starch and 0.09 watt/g starch have considerably higher from electric energy to the transformation efficiency of effective biomass.
As shown in Figure 5, compared with using common white light, the present invention is radiated at using feux rouges and red-blue-green mixed light
When cultivating few root duckweed xj3 under the conditions of eutrophy, in the case where being basically unchanged to total phosphorus in water removal rate, to total in water body
Nitrogen removal efficiency, which has, to be obviously improved, and the method for the invention can purifying water body while increasing duckweed starch yield.
Comparative example 2：Common white LED lamp+oligotrophic CMC model
Few root duckweed xj3 is inoculated in by 100% coverage in 250mL tap water, using common white LED lamp as light
Source, 25 DEG C according to photon hypothesis be 110 μm of ol/m2The illumination condition full exposure culture of/s, is mended with distilled water daily
The water evaporated is filled to former liquid level, is cultivated 5 days, and few root duckweed xj3 is harvested.
The dry of the few root duckweed xj3 harvested in this comparative example is measured and calculated using experiment condition identical with comparative example 1
Substance accumulating rate and content of starch, the results show that 5 days average dry substance accumulation speeds of few root duckweed xj3 are 5.42g/
m2/ d, content of starch 31.52%, starch accumulation speed are 8.94t/hac/y.
Embodiment 4：Blue LED lamp+oligotrophic CMC model
Few root duckweed xj3 is inoculated in by 100% coverage in 250mL tap water, using blue LED lamp as light source,
25 DEG C according to photon hypothesis be 110 μm of ol/m2The illumination condition full exposure culture of/s is supplemented with distilled water steam daily
The water that hair falls is cultivated 5 days to former liquid level, harvests few root duckweed xj3.
The dry of the few root duckweed xj3 harvested in the present embodiment is measured and calculated using experiment condition identical with comparative example 1
Substance accumulating rate and content of starch, the results show that 5 days average dry substance accumulation speeds of few root duckweed xj3 are 6.99g/
m2/ d, content of starch 47.23%, starch accumulation speed are 16.39t/hac/y.
Embodiment 5：Red LED lamp+oligotrophic CMC model
Few root duckweed xj3 is inoculated in by 100% coverage in 250mL tap water, using red LED lamp as light source,
25 DEG C according to photon hypothesis be 110 μm of ol/m2The illumination condition full exposure culture of/s is supplemented with distilled water steam daily
The water that hair falls is cultivated 5 days to former liquid level, harvests few root duckweed xj3.
The dry of the few root duckweed xj3 harvested in the present embodiment is measured and calculated using experiment condition identical with comparative example 1
Substance accumulating rate and content of starch, the results show that 5 days average dry substance accumulation speeds of few root duckweed xj3 are 7.26g/
m2/ d, content of starch 53.28%, starch accumulation speed are 19.20t/hac/y.
Embodiment 6：R/B/G:4/2/1 mixed light LED light+oligotrophic CMC model
Few root duckweed xj3 is inoculated in by 100% coverage in 250mL tap water, using the light of feux rouges, blue light and green light
Quantum flux density ratio is 4:2:1 red-blue-green mixed light LED light is according to photon hypothesis as light source, at 25 DEG C
110μmol/m2The illumination condition full exposure culture of/s, the water evaporated daily with distilled water supplement cultivate 5 to former liquid level
It, harvests few root duckweed xj3.
The dry of the few root duckweed xj3 harvested in the present embodiment is measured and calculated using experiment condition identical with comparative example 1
Substance accumulating rate and content of starch, the results show that 5 days average dry substance accumulation speeds of few root duckweed xj3 are 8.40g/
m2/ d, content of starch 61.34%, starch accumulation speed are 24.79t/hac/y.
By Fig. 6~8 it is found that using blue light, feux rouges and red-blue-green mixed light irradiation culture 5 under the conditions of oligotrophic
It, few root duckweed xj3 dry substance accumulation speeds that are averaged have reached 6.99~8.4g/m2/ d, content of starch be 47.23%~
61.34%, and white light culture 5 days is used, few root duckweed xj3 is averaged dry substance accumulation speed as 5.42g/m2/ d, starch
Content is only 31.52%, and starch yield has increased to 24.7t/hac/y by 8.94t/hac/y.Illustrate the method for the invention energy
The dry substance accumulation speed and content of starch for improving duckweed simultaneously, effectively improve the starch yield of duckweed.
Comparative example 3：Common white LED lamp+eutrophy CMC model
Duckweed is inoculated in by 100% coverage in the 1/5Hoagland nutrient solutions of 250mL, 1/5Hoagland nutrient solutions
In total nitrogen concentration be 70mg/L, total phosphorus concentration 30mg/L, using common white LED lamp as light source, at 25 DEG C according to light
Quantum flux density is 110 μm of ol/m2The illumination condition full exposure culture of/s, daily with the distilled water water that evaporates of supplement to original
Liquid level is cultivated 5 days, and duckweed is harvested.
The dry-matter accumulation speed of harvest duckweed in the present embodiment is measured and calculated using experiment condition identical with comparative example 1
Degree and content of starch, the results show that 5 days average dry substance accumulation speeds of duckweed are 5.00g/m2/ d, duckweed content of starch are
4.18%, duckweed starch accumulation speed is 0.92t/hac/y.
Embodiment 7：Blue LED lamp+eutrophy CMC model
Duckweed is inoculated in by 100% coverage in the 1/5Hoagland nutrient solutions of 250mL, 1/5Hoagland nutrient solutions
In total nitrogen concentration be 70mg/L, total phosphorus concentration 30mg/L, using blue LED lamp as light source, at 25 DEG C according to light quantum
Flux density is 110 μm of ol/m2The illumination condition full exposure culture of/s, daily with the distilled water water that evaporates of supplement to stoste face
Highly, it cultivates 5 days, harvests duckweed.
The dry-matter accumulation speed of harvest duckweed in the present embodiment is measured and calculated using experiment condition identical with comparative example 1
Degree and content of starch, the results show that 5 days average dry substance accumulation speeds of duckweed are 7.81g/m2/ d, duckweed content of starch are
17.72%, duckweed starch accumulation speed is 7.08t/hac/y.
Embodiment 8：Red LED lamp+eutrophy CMC model
Duckweed is inoculated in by 100% coverage in the 1/5Hoagland nutrient solutions of 250mL, 1/5Hoagland nutrient solutions
In total nitrogen concentration be 70mg/L, total phosphorus concentration 30mg/L, using common red LED lamp as light source, at 25 DEG C according to light
Quantum flux density is 110 μm of ol/m2The illumination condition full exposure culture of/s, daily with the distilled water water that evaporates of supplement to original
Liquid level is cultivated 5 days, and duckweed is harvested.
The dry-matter accumulation speed of harvest duckweed in the present embodiment is measured and calculated using experiment condition identical with comparative example 1
Degree and content of starch, the results show that 5 days average dry substance accumulation speeds of duckweed are 8.51g/m2/ d, duckweed content of starch are
22.46%, duckweed starch accumulation speed is 9.53t/hac/y.
Embodiment 9：R/B/G:4/2/1 mixed light LED light+eutrophy CMC model
Duckweed is inoculated in by 100% coverage in the 1/5Hoagland nutrient solutions of 250mL, 1/5Hoagland nutrient solutions
In total nitrogen concentration be 70mg/L, total phosphorus concentration 30mg/L, use the photon hypothesis ratio of feux rouges, blue light and green light for
4:2:1 red-blue-green mixed light LED light according to photon hypothesis is 110 μm of ol/m at 25 DEG C as light source2The light of/s
According to condition full exposure culture, the water evaporated daily with distilled water supplement is cultivated 5 days to former liquid level, harvests duckweed.
The dry-matter accumulation speed of harvest duckweed in the present embodiment is measured and calculated using experiment condition identical with comparative example 1
Degree and content of starch, the results show that 5 days average dry substance accumulation speeds of duckweed are 9.39g/m2/ d, duckweed content of starch are
21.94%, duckweed starch accumulation speed is 10.14t/hac/y.
By Fig. 9~11 it is found that using blue light, feux rouges and red-blue-green mixed light irradiation culture 5 under the conditions of eutrophy
It, the duckweed dry substance accumulation speed that is averaged has reached 7.81~9.39g/m2/ d, content of starch are 17.72%~22.46%, and
Using white light culture 5 days, duckweed was averaged dry substance accumulation speed as 5.00g/m2/ d, duckweed content of starch are only
4.18%, duckweed starch yield has increased to 10.14t/hac/y by 0.92t/hac/y, illustrates that the method for the invention is not limited to
In few root duckweed, the starch yield of duckweed can be also effectively improved.
Claims (9)
1. a kind of method improving duckweed starch yield by optimizing spectral composition, it is characterised in that duckweed is inoculated in water body
In, it is cultivated 5~14 days under the irradiation condition of feux rouges, blue light or red-blue-green mixed light, harvests duckweed.
2. the method for improving duckweed starch yield by optimizing spectral composition according to claim 1, which is characterized in that according to
The photon hypothesis of feux rouges, blue light or red-blue-green mixed light is 80~200 μm of ol/m2The illumination condition culture of/s is floating
Duckweed.
3. the method according to claim 1 or claim 2 for improving duckweed starch yield by optimizing spectral composition, it is characterised in that red
The peak wavelength of light is 660nm, and the peak wavelength of blue light is 450nm, and the peak wavelength of green light is 520nm.
4. the method for improving duckweed starch yield by optimizing spectral composition according to claim 3, it is characterised in that feux rouges
Peak width at half height be no more than 15nm, the peak width at half height of blue light is no more than 20nm, and the peak width at half height of green light is no more than 15nm.
5. the method according to claim 1 or claim 2 for improving duckweed starch yield by optimizing spectral composition, which is characterized in that
Using red-blue-green mixed light irradiation when, the photon hypothesis of feux rouges, blue light and green light in red-blue-green mixed light
The ratio between be (3.5~4.5):(1.5~2.5):(0.5~1.5).
6. the method for improving duckweed starch yield by optimizing spectral composition according to claim 3, which is characterized in that use
When the irradiation of red-blue-green mixed light, the ratio between the photon hypothesis of feux rouges, blue light and green light in red-blue-green mixed light
For (3.5~4.5):(1.5~2.5):(0.5~1.5).
7. the method for improving duckweed starch yield by optimizing spectral composition according to claim 4, which is characterized in that use
When the irradiation of red-blue-green mixed light, the ratio between the photon hypothesis of feux rouges, blue light and green light in red-blue-green mixed light
For (3.5~4.5):(1.5~2.5):(0.5~1.5).
8. the method according to claim 1 or claim 2 for improving duckweed starch yield by optimizing spectral composition, it is characterised in that adopt
Feux rouges is provided with red LED lamp, blue light is provided using blue LED lamp, by red LED lamp, blue LED lamp and green light LED lamp group
It closes and red-blue-green mixed light is provided.
9. the method according to claim 1 or claim 2 for improving duckweed starch yield by optimizing spectral composition, it is characterised in that press
According to 80%~100% coverage rate by duckweed kind in water body.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102766578A (en) * | 2011-10-20 | 2012-11-07 | 烟台华融生物科技有限公司 | Cultivating and producing method for haematococcus pluvialis |
| CN103098642A (en) * | 2013-03-05 | 2013-05-15 | 万世凤 | Duckweed cultivation method |
| CN105454026A (en) * | 2015-11-19 | 2016-04-06 | 中国科学院青岛生物能源与过程研究所 | Duckweed stereoscopic culturing apparatus |
| WO2017024252A1 (en) * | 2015-08-06 | 2017-02-09 | Coats Agri Aloe, Llc | Compositions and methods to increase plant yield |
| CN106416968A (en) * | 2016-07-20 | 2017-02-22 | 华南农业大学 | Method for hydroponically culturing lettuce by means of LED light source |
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2017
- 2017-09-20 CN CN201710853398.7A patent/CN108323427B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102766578A (en) * | 2011-10-20 | 2012-11-07 | 烟台华融生物科技有限公司 | Cultivating and producing method for haematococcus pluvialis |
| CN103098642A (en) * | 2013-03-05 | 2013-05-15 | 万世凤 | Duckweed cultivation method |
| WO2017024252A1 (en) * | 2015-08-06 | 2017-02-09 | Coats Agri Aloe, Llc | Compositions and methods to increase plant yield |
| CN105454026A (en) * | 2015-11-19 | 2016-04-06 | 中国科学院青岛生物能源与过程研究所 | Duckweed stereoscopic culturing apparatus |
| CN106416968A (en) * | 2016-07-20 | 2017-02-22 | 华南农业大学 | Method for hydroponically culturing lettuce by means of LED light source |
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