CN107548982B - Method for improving total starch yield of duckweed by promoting dormancy formation - Google Patents
Method for improving total starch yield of duckweed by promoting dormancy formation Download PDFInfo
- Publication number
- CN107548982B CN107548982B CN201710853348.9A CN201710853348A CN107548982B CN 107548982 B CN107548982 B CN 107548982B CN 201710853348 A CN201710853348 A CN 201710853348A CN 107548982 B CN107548982 B CN 107548982B
- Authority
- CN
- China
- Prior art keywords
- light
- duckweed
- red
- blue
- starch
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/14—Measures for saving energy, e.g. in green houses
Abstract
The invention belongs to the field of aquatic energy plant production, and provides a method for improving total starch yield of duckweed by promoting dormancy formation, which comprises the steps of inoculating duckweed in a water body, culturing for 14-30 days under the irradiation condition of red light, blue light or red-blue mixed light, and harvesting duckweed dormancy bodies and fronds. The method can save cost, improve production efficiency, effectively increase total starch yield of duckweed, is not limited by water nutrition, and has universal applicability.
Description
Technical Field
The invention belongs to the field of aquatic energy plant production, and particularly relates to a method for improving total starch yield of duckweed by promoting dormancy formation.
Background
Lemna polyrhiza of the Lemnaceae family can produce a vegetative propagule, i.e., a quiescent body (turion), that is distinctly different from normal fronds. Both the dormant bodies and the normal fronds are differentiated from the same leaf primordia, and the formation of dormant bodies means that the developmental state of duckweed is changed. The main characteristic of dormant bodies is that the starch content is high and can generally reach more than 60% (dry weight), while the starch content of corresponding fronds is usually about 4-10%, and can only reach 30% at most even under induction conditions. The grain size of the starch granules of the duckweed dormancy bodies is about 4 mu m, and is smaller than that of the starch granules of corn, rice and the like, so that the duckweed dormancy bodies are easier to hydrolyze and are used for producing biofuel. Because the dormant body has less ventilating tissues and higher density, the dormant body can be separated from the parent body after being matured, and can sink to the water bottom, so that the dormant body is easy to separate from the floating parent body for harvesting. Meanwhile, compared with fronds, the dormant bodies have higher mass/area ratio and lower water content, so that the drying treatment of the dormant bodies after harvesting is more economical. These characteristics make the duckweed-dormant bodies a very potential biofuel production raw material. Although the starch content of the duckweed-derived dormant bodies is high, the dry matter accumulation rate is low, the yield is low, and actually the starch yield of the duckweed-derived dormant bodies is relatively low, which becomes a key limiting factor for the practical application of the duckweed-derived dormant bodies in the field of biofuel production.
Currently, methods for promoting the production of duckweed-dormant bodies can be divided into two categories. One method is to increase the number of fronds by increasing their photosynthesis, and thus increase the number of dormant bodies, by adding sucrose to a water body, culturing with high light intensity, and culturing at high CO2Although the method has obvious effect on increasing the number of dormant bodies in culture under the concentration condition, the method has the disadvantages of complex operation, long time consumption, low production efficiency, high production cost and no economy. Another group of methods, which mainly promote the formation of dormant bodies by means of abiotic stress, specifically comprises nutrition deficiency, low temperature, abscisic acid spraying and the like, has very limited effect on the increase of the number of dormant bodies, and simultaneously inhibits the growth of the fronds, so that the starch yield of the fronds is reducedWhile in practice the total starch yield of duckweed clusters is still low.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provide a method for improving the total duckweed starch yield by promoting dormancy body formation so as to improve the total duckweed starch yield on the basis of improving the production efficiency and reducing the production cost.
The method for improving the total starch yield of the duckweed by promoting the formation of the dormant body provided by the invention comprises the steps of inoculating the duckweed into a water body, culturing for 14-30 days under the irradiation condition of red light, blue light or red-blue mixed light, and harvesting the duckweed dormant body and the fronds.
In the method, the light quantum flux density (PPFD) of red light, blue light or red-blue mixed light is 80-200 mu mol/m2Culturing duckweed under the illumination condition of/s.
In the method, the peak wavelength of the red light is 660nm, the peak wavelength of the blue light is 450nm, and the narrower the half-height peak widths of the red light and the blue light are, the better the improvement effect of the method on the total yield of the duckweed starch is, preferably, the half-height peak width of the red light is not more than 15nm, and the half-height peak width of the blue light is not more than 20 nm.
In the method, when the red-blue mixed light is used for illumination, the ratio of the light quantum flux density of the red light to the light quantum flux density of the blue light in the red-blue mixed light is 1 (0.25-1).
In the method, the red light LED lamp is adopted to provide red light, the blue light LED lamp is adopted to provide blue light, and the red light LED lamp and the blue light LED lamp are combined to provide red-blue mixed light.
In the method, in order to improve the total starch yield of the duckweed to the maximum extent, the duckweed is preferably planted in the water body according to the coverage rate of 80-100%.
In the method, the duckweed is a plurality of duckweed, in particular a plurality of duckweed gx 1.
Compared with the prior art, the invention has the following beneficial effects:
1. the invention provides a method for improving total duckweed starch yield by promoting dormancy body formation, which induces the development state of duckweed to change by using a monochromatic red light or blue light LED lamp or a red-blue mixed light LED lamp as a light source, improves the photosynthetic efficiency of the duckweed, and can greatly improve the starch yield of a plurality of duckweed dormancy bodies and the total duckweed starch yield.
2. Experiments show that when the method is used for culturing the duckweed under the condition of rich nutrition, the starch accumulation speed of the duckweed dormant bodies and the fronds is improved, and the total starch accumulation speed reaches 1.41-2.34 g/m2D, which is significantly higher than the total starch accumulation speed of 0.49g/m under the corresponding white light irradiation condition2D, culturing a plurality of duckweeds under oligotrophic conditions, simultaneously improving the starch accumulation speed of duckweed dormant bodies and fronds, and leading the total starch accumulation speed to reach 3.04-4.18 g/m2D, which is significantly higher than the total starch accumulation rate of 2.5g/m under corresponding white light irradiation conditions2And d, the method can effectively improve the starch yield of the duckweed without being limited by the water nutrition condition, and has universal applicability.
3. The light quantum flux density is 110 mu mol/m2Compared with a white light LED lamp as a light source, the method of the invention adopts a monochromatic red light or blue light LED lamp or a red-blue mixed light LED lamp as the light source, can reduce the electric energy consumption by 12.56% at most while increasing the dormant body starch yield and the total duckweed starch yield, and has the advantage of saving illumination cost.
Drawings
FIG. 1 is a graph comparing the dry matter accumulation rates of dormant bodies in comparative example 1 and examples 1 to 6.
FIG. 2 is a graph comparing the rate of accumulation of dry matter in the fronds in comparative example 1 and examples 1-6.
FIG. 3 is a graph comparing the starch content of the dormant bodies in comparative example 1 and examples 1 to 6.
FIG. 4 is a graph comparing the starch content of the fronds in comparative example 1 and examples 1-6.
FIG. 5 is a graph comparing the total starch accumulation rates in comparative example 1 and examples 1-6.
FIG. 6 is a graph comparing the dry matter accumulation rates of dormant bodies in comparative example 2 and examples 7 to 12.
FIG. 7 is a graph comparing the rate of accumulation of dry matter in the fronds in comparative example 2 and examples 7-12.
FIG. 8 is a graph comparing the starch content of the dormant bodies in comparative example 2 and examples 7 to 12.
FIG. 9 is a graph comparing the starch content of the fronds in comparative example 2 and examples 7-12.
FIG. 10 is a graph comparing the total starch accumulation rates in comparative example 2 and examples 7-12.
Detailed Description
The method of the present invention for increasing the total starch yield of duckweed by promoting dormancy formation is further illustrated by the following examples and comparative examples.
In the following examples and comparative examples, the determination of duckweed starch content is described in Zhang L, Chen Q, Jin Y, et al, energy-saving direct ethanol production from vision reduction map of sweet spot to top high quality (VHG), Fuel processing technology 2010,91(12), 1845-1850. The specific determination method comprises the following steps: respectively crushing dried duckweed fronds and dormant bodies into powder, weighing 0.03-0.06 g of duckweed dry powder, placing the powder into a 250mL ground conical flask, adding 30mL of 6mol/L HCl solution and 100mL of distilled water, installing a condensing tube, and placing the solution into a boiling water bath for refluxing for 2 h. And (4) immediately cooling the mixture by using flowing water after the reflux is finished, and adding NaOH to adjust the pH value of the hydrolysate to 7 after the duckweed sample hydrolysate is cooled to room temperature. Then adding 20mL of 20 wt% lead acetate solution, shaking uniformly, standing for 10min, transferring to a 500mL volumetric flask, adding distilled water to a constant volume of 500mL, filtering, discarding the primary filtrate, collecting 5mL of filtrate, passing through a preactivated reverse phase C18 solid phase extraction column, discarding the primary 1-2 mL, collecting the subsequent 3-4 mL, and filtering with a 0.22 mu m water-based filter membrane. The glucose content of the filtrate was measured by HPLC, and the starch content of the duckweed fronds and dormant bodies was calculated from the starch content ═ glucose content/1.1.
In the following examples, the preparation of each solution was as follows:
the preparation method of the Hoagland culture solution comprises the following steps: (1) a, B, C, D, E, F kinds of mother liquor were prepared from distilled water according to the concentration of each reagent in the mother liquor formulation shown in the following table, wherein, when preparing the mother liquor A, Ca (NO) was first dissolved in 6N HCl solution3)2·4H2O、KNO3、KH2PO4Dissolving, and then adding distilled water to prepare target concentration; when preparing the mother liquor D, dissolving EDTA by 6N KOH solution, and then adding distilled water to prepare the target concentration. (2) Hoagland broth was prepared with distilled water according to the amounts of each mother liquor added per liter of Hoagland broth described in the table below, and the pH of the Hoagland broth was adjusted to 5.0 with HCl and NaOH. The concentration of the N element in the prepared Hoagland culture solution is 349.73mg/L, and the concentration of the P element is 154.89 mg/L.
1/5Hoagland culture solution preparation method: mixing a certain amount of Hoagland culture solution with distilled water with the volume 4 times of that of the Hoagland culture solution uniformly to obtain the compound.
The duckweed used in each of the following examples and comparative examples was a plurality of duckweed gx1 collected from Guangxi province of China.
The light source adopted in each proportion is a common white light LED lamp, and the light quality composition is as follows: blue/green/red (B/G/R) 37/45/28, which is the ratio of the photon flux density of blue, green and red light. In the following embodiments, red light and blue light are provided by a red LED lamp and a blue LED lamp, respectively, and the red LED lamp and the blue LED lamp are combined into a red-blue mixed light LED lamp to provide red-blue mixed light. The peak wavelength of the red light is 660nm, the half-height peak width is 15nm, the peak wavelength of the blue light is 450nm, and the half-height peak width is 20 nm. When the red-blue mixed light is provided, the red light LED lamp and the blue light LED lamp are respectively combined to form the light quantum flux density ratio of red light to blue light according to different application requirements in each embodiment: red-blue mixed light LED lamps of red/blue (R/B) 1/2, 1/1, 2/1 and 4/1.
In the following comparative examples and comparative examples, the optical quantum flux density was 110. mu. mol/m2And culturing the duckweed under full illumination under the illumination condition of/s, wherein the light quantum flux density refers to the optical density of the corresponding light source on the surface of the water body inoculated with the duckweed.
Comparative example 1: ordinary white light LED lamp and rich nutrition condition culture
The duckweed is inoculated into 250mL of 1/5Hoagland nutrient solution according to 100 percent coverage, the total nitrogen concentration in the 1/5Hoagland nutrient solution is 70mg/L, the total phosphorus concentration is 30mg/L, a common white light LED lamp is adopted as a light source, and the luminous quantum flux density is 110 mu mol/m at 25 DEG C2And (3) performing full-light culture under the illumination condition of/s, supplementing evaporated water to the height of the original liquid level with distilled water every day, culturing for 14 days, and harvesting thallus and dormant bodies respectively.
And (3) respectively washing the harvested fronds and the dormant bodies with distilled water for three times, placing the obtained fronds and the dormant bodies in a filter bag, removing free water by a spin dryer, drying the obtained product in an oven at 60 ℃ overnight until the weight is constant, weighing and recording, and calculating the dry matter accumulation rate of the fronds and the dormant bodies. And grinding the dried sample into powder, weighing a proper amount of the powder, measuring the glucose content by using an HPLC method after acidolysis, and calculating the starch content. The results showed that the average rate of accumulation of dry matter in the dormant body was 0.32g/m2D, dormant starch content 69.49%, dry matter accumulation rate of thallus 5.76g/m2The content of thallus starch is 4.82%, and the total starch accumulation speed of duckweed is 0.49g/m2/d。
Example 1: blue light LED lamp + eutrophication condition culture
The duckweed is inoculated into 250mL of 1/5Hoagland nutrient solution according to 100 percent coverage, the total nitrogen concentration in the 1/5Hoagland nutrient solution is 70mg/L, the total phosphorus concentration is 30mg/L, a blue light LED lamp is adopted as a light source, and the light quantum flux density is 110 mu mol/m at 25 DEG C2And (3) performing full-light culture under the illumination condition of/s, supplementing evaporated water to the height of the original liquid level with distilled water every day, culturing for 14 days, and harvesting thallus and dormant bodies respectively.
Determination and calculation of harvested fronds and dormancy in this example using the same experimental conditions as in comparative example 1The dry matter accumulation rate of the bodies and the starch content show that the average dry matter accumulation rate of dormant bodies is 1.02g/m2D, dormant starch content 72.00%, dry matter accumulation rate of thallus 6.09g/m2The content of thallus starch is 10.94 percent, and the total accumulation speed of duckweed starch is 1.49g/m2/d。
Example 2: red light LED lamp and nutrient enrichment condition culture
The duckweed is inoculated into 250mL of 1/5Hoagland nutrient solution according to 100 percent coverage, the total nitrogen concentration in the 1/5Hoagland nutrient solution is 70mg/L, the total phosphorus concentration is 30mg/L, a red light LED lamp is adopted as a light source, and the luminous quantum flux density is 110 mu mol/m at 25 DEG C2And (3) performing full-light culture under the illumination condition of/s, supplementing evaporated water to the height of the original liquid level with distilled water every day, culturing for 14 days, and harvesting thallus and dormant bodies respectively.
The dry matter accumulation rates and starch contents of the fronds and dormant bodies harvested in this example were measured and calculated using the same experimental conditions as in comparative example 1, and the results showed that the average dry matter accumulation rate of dormant bodies was 2.01g/m2The dormant starch content is 73.35%, and the accumulation rate of dry matter in thallus is 5.99g/m2The content of thallus starch is 7.32 percent, and the total accumulation speed of duckweed starch is 1.94g/m2/d。
Example 3: R/B:1/2 mixed light LED lamp and eutrophic condition culture
The duckweed is inoculated into 250mL of 1/5Hoagland nutrient solution according to 100 percent coverage, the total nitrogen concentration in the 1/5Hoagland nutrient solution is 70mg/L, the total phosphorus concentration is 30mg/L, a red-blue mixed light LED lamp with the ratio of the luminous quantum flux density of red light to blue light of 1:2 is adopted as a light source, and the luminous quantum flux density is 110 mu mol/m at 25 DEG C2And (3) performing full-light culture under the illumination condition of/s, supplementing evaporated water to the height of the original liquid level with distilled water every day, culturing for 14 days, and harvesting thallus and dormant bodies respectively.
The dry matter accumulation rates and starch contents of the fronds and dormant bodies harvested in this example were determined and calculated using the same experimental conditions as in comparative example 1, and the results show that the average dry matter accumulation rate of the dormant bodiesThe degree is 1.39g/m2D, dormant starch content 75.04%, dry matter accumulation rate of thallus 7.21g/m2The content of thallus starch is 8.09%, and the total accumulation speed of duckweed starch is 1.68g/m2/d。
Example 4: R/B:1/1 mixed light LED lamp and eutrophic condition culture
The duckweed is inoculated into 250mL of 1/5Hoagland nutrient solution according to 100 percent coverage, the total nitrogen concentration in the 1/5Hoagland nutrient solution is 70mg/L, the total phosphorus concentration is 30mg/L, a red-blue mixed light LED lamp with the ratio of the luminous quantum flux density of red light to blue light of 1:1 is adopted as a light source, and the luminous quantum flux density is 110 mu mol/m at 25 DEG C2And (3) performing full-light culture under the illumination condition of/s, supplementing evaporated water to the height of the original liquid level with distilled water every day, culturing for 14 days, and harvesting thallus and dormant bodies respectively.
The dry matter accumulation rates and starch contents of the fronds and dormant bodies harvested in this example were measured and calculated using the same experimental conditions as in comparative example 1, and the results showed that the average dry matter accumulation rate of dormant bodies was 0.98g/m2The dormant starch content is 75.54%, and the accumulation rate of dry matter in thallus is 6.92g/m2The content of thallus starch is 8.89%, and the total accumulation speed of duckweed starch is 1.41g/m2/d。
Example 5: R/B:2/1 mixed light LED lamp and eutrophic condition culture
The duckweed is inoculated into 250mL of 1/5Hoagland nutrient solution according to 100 percent coverage, the total nitrogen concentration in the 1/5Hoagland nutrient solution is 70mg/L, the total phosphorus concentration is 30mg/L, a red-blue mixed light LED lamp with the ratio of the luminous quantum flux density of red light to blue light of 2:1 is adopted as a light source, and the luminous quantum flux density is 110 mu mol/m at 25 DEG C2And (3) performing full-light culture under the illumination condition of/s, supplementing evaporated water to the height of the original liquid level with distilled water every day, culturing for 14 days, and harvesting thallus and dormant bodies respectively.
The dry matter accumulation rates and starch contents of the fronds and dormant bodies harvested in this example were measured and calculated using the same experimental conditions as in comparative example 1, and the results showed that the average dry matter accumulation rate of dormant bodies was 2.17g/m2D, dormant starch content 71.12%, dry matter accumulation rate of thallus 6.77g/m2The content of thallus starch is 9.81 percent, and the total accumulation speed of duckweed starch is 2.28g/m2/d。
Example 6: R/B:4/1 mixed light LED lamp and eutrophic condition culture
The duckweed is inoculated into 250mL of 1/5Hoagland nutrient solution according to 100 percent coverage, the total nitrogen concentration in the 1/5Hoagland nutrient solution is 70mg/L, the total phosphorus concentration is 30mg/L, a red-blue mixed light LED lamp with the ratio of the luminous quantum flux density of red light to blue light of 4:1 is adopted as a light source, and the luminous quantum flux density is 110 mu mol/m at 25 DEG C2And (3) performing full-light culture under the illumination condition of/s, supplementing evaporated water to the height of the original liquid level with distilled water every day, culturing for 14 days, and harvesting thallus and dormant bodies respectively.
The dry matter accumulation rates and starch contents of the fronds and dormant bodies harvested in this example were determined and calculated using the same experimental conditions as in comparative example 1, and the results showed that the average dry matter accumulation rate of dormant bodies was 2.09g/m2The dormant starch content was 74.92%, and the accumulation rate of dry matter in fronds was 6.89g/m2The content of thallus starch is 10.12 percent, and the total accumulation speed of duckweed starch is 2.34g/m2/d。
As can be seen from FIGS. 1 and 3, the average dry matter accumulation rate of the dormant body reaches 0.98-2.17 g/m after the culture is performed for 14 days under the condition of rich nutrition by irradiation of red light, blue light and red-blue mixed light2The dormant body starch content is 71.12% -75.54%, while the average dry matter accumulation speed of the dormant body is only 0.32g/m after the dormant body is irradiated by white light for 14 days2The dormant body starch content is 69.49%, which shows that the method of the invention can effectively improve the accumulation speed of the dry matter of the dormant body while improving the dormant body starch content. The method provided by the invention can be used for culturing the duckweed under the condition of rich nutrition, and the starch yield of the dormant body can be obviously improved.
As can be seen from FIGS. 2 and 4, the average dry matter accumulation rate of the thallus is 5.99-7.21 g/m when the thallus is cultured for 14 days under the condition of rich nutrition by irradiation of red light, blue light and red-blue mixed light2D, lobate precipitationThe powder content is 7.32-10.94%, and the average dry matter accumulation rate of thallus is 5.76g/m after white light irradiation culture for 14 days2The thallus starch content is 4.82%, which shows that the method of the invention can simultaneously improve the thallus starch content and the dry matter accumulation speed. The method provided by the invention can be used for culturing the duckweed under the condition of rich nutrition, so that the starch yield of the thallus can be improved.
As can be seen from FIG. 5, the total starch accumulation rate of the duckweed reaches 1.41-2.34 g/m after the culture is performed for 14 days under the condition of rich nutrition by adopting red light, blue light and red-blue mixed light for irradiation2The total starch accumulation rate of the duckweed cultured for 14 days by white light irradiation is only 0.49g/m2And d, the method provided by the invention can effectively improve the total starch yield of the duckweed.
Comparative example 2: ordinary white light LED lamp and oligotrophic condition culture
The duckweed is inoculated into 250mL of tap water according to 100 percent coverage, a common white light LED lamp is adopted as a light source, and the light quantum flux density is 110 mu mol/m at 25 DEG C2And (3) performing full-light culture under the illumination condition of/s, supplementing evaporated water to the height of the original liquid level with distilled water every day, culturing for 14 days, and harvesting thallus and dormant bodies respectively.
The dry matter accumulation rates and starch contents of the fronds and dormant bodies harvested in the comparative example were measured and calculated using the same experimental conditions as in comparative example 1, and the results showed that the average dry matter accumulation rate of the dormant bodies for 14 days was 3.20g/m2D, dormant starch content 66.29%, dry matter accumulation rate of thallus 2.42g/m2D, the content of the thallus starch is 15.98 percent, and the total accumulation speed of the duckweed starch is 2.50g/m2/d。
Example 7: blue light LED lamp + oligotrophic condition culture
The duckweed is inoculated into 250mL of tap water according to 100 percent coverage, a blue light LED lamp is adopted as a light source, and the luminous quantum flux density is 110 mu mol/m at 25 DEG C2And (3) performing full-light culture under the illumination condition of/s, supplementing evaporated water to the height of the original liquid level with distilled water every day, culturing for 14 days, and harvesting thallus and dormant bodies respectively.
The dry matter accumulation rates and starch contents of the fronds and dormant bodies harvested in this example were measured and calculated using the same experimental conditions as in comparative example 1, and the results showed that the average dry matter accumulation rate of the dormant bodies for 14 days was 3.67g/m2D, dormant starch content 71.54%, dry matter accumulation rate of thallus 2.69g/m2The content of thallus starch is 23.19%, and the total accumulation rate of duckweed starch is 3.25g/m2/d。
Example 8: red light LED lamp and oligotrophic condition culture
The duckweed is inoculated into 250mL of tap water according to 100 percent coverage, a red light LED lamp is adopted as a light source, and the luminous flux density is 110 mu mol/m at 25 DEG C2And (3) performing full-light culture under the illumination condition of/s, supplementing evaporated water to the height of the original liquid level with distilled water every day, culturing for 14 days, and harvesting thallus and dormant bodies respectively.
The dry matter accumulation rates and starch contents of the fronds and the dormant bodies harvested in this example were measured and calculated using the same experimental conditions as in comparative example 1, and the results showed that the average dry matter accumulation rate of the dormant bodies for 14 days was 3.59g/m2D, dormant starch content 74.06%, dry matter accumulation rate of thallus 2.35g/m2The content of thallus starch is 16.19%, and the total starch accumulation speed of duckweed is 3.04g/m2/d。
Example 9: cultivation under conditions of mixed light LED lamp and oligotrophic culture with R/B of 1/2
The duckweed is inoculated into 250mL of tap water according to 100 percent coverage, a red-blue mixed light LED lamp with the light quantum flux density ratio of red light to blue light of 1:2 is adopted as a light source, and the light quantum flux density is 110 mu mol/m at 25 DEG C2And (3) performing full-light culture under the illumination condition of/s, supplementing evaporated water to the height of the original liquid level with distilled water every day, culturing for 14 days, and harvesting thallus and dormant bodies respectively.
The dry matter accumulation rates and starch contents of the fronds and dormant bodies harvested in this example were measured and calculated using the same experimental conditions as in comparative example 1, and the results showed that the average dry matter accumulation rate of 14-day dormant bodies was 4.79g/m2D, sleepThe bulk starch content was 76.51%, and the accumulation rate of dry matter in fronds was 2.75g/m2The content of thallus starch is 18.45 percent, and the total accumulation speed of duckweed starch is 4.18g/m2/d。
Example 10: cultivation under conditions of mixed light LED lamp and oligotrophic culture with R/B of 1/1
The duckweed is inoculated into 250mL of tap water according to 100 percent coverage, a red-blue mixed light LED lamp with the light quantum flux density ratio of red light to blue light of 1:1 is adopted as a light source, and the light quantum flux density is 110 mu mol/m at 25 DEG C2And (3) performing full-light culture under the illumination condition of/s, supplementing evaporated water to the height of the original liquid level with distilled water every day, culturing for 14 days, and harvesting thallus and dormant bodies respectively.
The dry matter accumulation rates and starch contents of the fronds and the dormant bodies harvested in this example were measured and calculated using the same experimental conditions as in comparative example 1, and the results showed that the average dry matter accumulation rate of the dormant bodies for 14 days was 4.59g/m2D, dormant starch content 71.64%, dry matter accumulation rate of thallus 2.72g/m2The content of thallus starch is 18.83%, and the total accumulation speed of duckweed starch is 3.79g/m2/d。
Example 11: cultivation under conditions of mixed light LED lamp and oligotrophic culture with R/B of 2/1
The duckweed is inoculated into 250mL of tap water according to 100 percent coverage, a red-blue mixed light LED lamp with the light quantum flux density ratio of red light to blue light of 2:1 is adopted as a light source, and the light quantum flux density is 110 mu mol/m at 25 DEG C2And (3) performing full-light culture under the illumination condition of/s, supplementing evaporated water to the height of the original liquid level with distilled water every day, culturing for 14 days, and harvesting thallus and dormant bodies respectively.
The dry matter accumulation rates and starch contents of the fronds and dormant bodies harvested in this example were measured and calculated using the same experimental conditions as in comparative example 1, and the results showed that the average dry matter accumulation rate of 14-day dormant bodies was 4.52g/m2D, dormant starch content 77.01%, dry matter accumulation rate of thallus 2.77g/m2The content of thallus starch is 19.79 percent, and the accumulation speed of the total duckweed starch is 4.03g/m2/d。
Example 12: cultivation under conditions of mixed light LED lamp and oligotrophic culture with R/B of 4/1
The duckweed is inoculated into 250mL of tap water according to 100 percent coverage, a red-blue mixed light LED lamp with the light quantum flux density ratio of red light to blue light of 4:1 is adopted as a light source, and the light quantum flux density is 110 mu mol/m at 25 DEG C2And (3) performing full-light culture under the illumination condition of/s, supplementing evaporated water to the height of the original liquid level with distilled water every day, culturing for 14 days, and harvesting thallus and dormant bodies respectively.
The dry matter accumulation rates and starch contents of the fronds and dormant bodies harvested in this example were measured and calculated using the same experimental conditions as in comparative example 1, and the results showed that the average dry matter accumulation rate of 14-day dormant bodies was 4.38g/m2The dormant starch content was 75.97%, and the accumulation rate of dry matter in fronds was 2.61g/m2The content of thallus starch is 18.41 percent, and the total accumulation speed of duckweed starch is 3.80g/m2/d。
As can be seen from FIGS. 6 and 8, the average dry matter accumulation rate of the dormant body is 3.59-4.79 g/m in 14 days of culture under oligotrophic conditions by irradiation with red light, blue light and red-blue mixed light2The dormant body starch content is 71.54-77.01%, and the average dry matter accumulation speed of the dormant body is only 3.2g/m after the dormant body is cultured for 14 days by white light irradiation2The dormant starch content is 66.29 percent, and the method can simultaneously improve the dormant starch content and the dry matter accumulation speed. The method provided by the invention can be used for culturing the duckweed under the oligotrophic condition, so that the starch yield of the dormant body can be effectively improved.
As can be seen from FIGS. 7 and 9, the average accumulation rate of dry matter in the fronds is 2.35-2.77 g/m in the culture under oligotrophic conditions for 14 days by irradiation with red light, blue light and red-blue mixed light2The content of the starch in the frond is 16.19-23.19%, and the average dry matter accumulation speed of the frond is 2.42g/m after the frond is cultured for 14 days by white light irradiation2And/d, the content of the thallus starch is 15.98 percent, and the method can improve the content of the thallus starch on the basis of keeping the accumulation speed of the dry matter of the thallus basically unchanged.
As can be seen from FIG. 10, the total starch accumulation rate of the duckweed reaches 3.04-4.18 g/m after the culture is performed for 14 days under the oligotrophic condition by adopting red light, blue light and red-blue mixed light for irradiation2The total starch accumulation rate of the duckweed cultured for 14 days by white light irradiation is only 2.5g/m2And d, the method provided by the invention can effectively improve the total starch yield of the duckweed.
Claims (5)
1. A method for improving total starch yield of duckweed by promoting dormancy body formation is characterized in that duckweed is inoculated in a water body, and the light quantum flux density of red light, blue light or red-blue mixed light is 80-200 mu mol/m2Culturing duckweed under the illumination condition of/s, culturing for 14-30 days under the illumination condition of red light, blue light or red-blue mixed light, and harvesting duckweed dormancy bodies and fronds;
when the red-blue mixed light is adopted for illumination, the ratio of the light quantum flux density of the red light to the light quantum flux density of the blue light in the red-blue mixed light is 1 (0.25-1); the duckweed is a plurality of duckweeds.
2. The method of claim 1, wherein the peak wavelength of red light is 660nm and the peak wavelength of blue light is 450 nm.
3. The method of claim 2, wherein the red light has a peak width at half maximum of no more than 15nm and the blue light has a peak width at half maximum of no more than 20 nm.
4. The method of claim 1, wherein red light is provided by a red LED lamp, blue light is provided by a blue LED lamp, and a combination of a red LED lamp and a blue LED lamp provides a red-blue mixture of light.
5. The method for increasing total duckweed starch production by promoting dormancy formation according to claim 1, wherein the duckweed is planted in the water at a coverage rate of 80% to 100%.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2017106712836 | 2017-08-08 | ||
| CN201710671283 | 2017-08-08 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107548982A CN107548982A (en) | 2018-01-09 |
| CN107548982B true CN107548982B (en) | 2020-03-06 |
Family
ID=60982240
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710853348.9A Active CN107548982B (en) | 2017-08-08 | 2017-09-20 | Method for improving total starch yield of duckweed by promoting dormancy formation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107548982B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108782204B (en) * | 2018-06-26 | 2020-08-14 | 中国科学院成都生物研究所 | Method for improving total starch yield of duckweed by using methylation inhibitor |
| CN112794920B (en) * | 2021-01-15 | 2022-01-11 | 江南大学 | Method for extracting duckweed dormant body starch |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102766578A (en) * | 2011-10-20 | 2012-11-07 | 烟台华融生物科技有限公司 | Cultivating and producing method for haematococcus pluvialis |
| CN103098642A (en) * | 2013-03-05 | 2013-05-15 | 万世凤 | Duckweed cultivation method |
| CN103609304A (en) * | 2013-11-22 | 2014-03-05 | 中国科学院成都生物研究所 | Method for quickly increasing content of starch in duckweed |
| CN104839025A (en) * | 2015-05-25 | 2015-08-19 | 北京大学深圳研究生院 | Culture method of high-starch duckweeds |
| CN105454026A (en) * | 2015-11-19 | 2016-04-06 | 中国科学院青岛生物能源与过程研究所 | Duckweed stereoscopic culturing apparatus |
| WO2017024252A1 (en) * | 2015-08-06 | 2017-02-09 | Coats Agri Aloe, Llc | Compositions and methods to increase plant yield |
-
2017
- 2017-09-20 CN CN201710853348.9A patent/CN107548982B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102766578A (en) * | 2011-10-20 | 2012-11-07 | 烟台华融生物科技有限公司 | Cultivating and producing method for haematococcus pluvialis |
| CN103098642A (en) * | 2013-03-05 | 2013-05-15 | 万世凤 | Duckweed cultivation method |
| CN103609304A (en) * | 2013-11-22 | 2014-03-05 | 中国科学院成都生物研究所 | Method for quickly increasing content of starch in duckweed |
| CN104839025A (en) * | 2015-05-25 | 2015-08-19 | 北京大学深圳研究生院 | Culture method of high-starch duckweeds |
| WO2017024252A1 (en) * | 2015-08-06 | 2017-02-09 | Coats Agri Aloe, Llc | Compositions and methods to increase plant yield |
| CN105454026A (en) * | 2015-11-19 | 2016-04-06 | 中国科学院青岛生物能源与过程研究所 | Duckweed stereoscopic culturing apparatus |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107548982A (en) | 2018-01-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107548982B (en) | Method for improving total starch yield of duckweed by promoting dormancy formation | |
| CN103609304B (en) | A kind of method of quick raising duckweed content of starch | |
| CN105594530B (en) | Agricultural method for controlling cadmium and enriching selenium of rice | |
| CN106007995A (en) | Preparation method and application of selenium-enriched fertilizer dedicated to tea | |
| CN103421642A (en) | Method for processing cider wine containing more ester | |
| CN102321518B (en) | Method for preparing vinegar with high amino acid and reducing sugar | |
| CN115093918A (en) | Hovenia acerba tea wine and brewing method thereof | |
| CN108967183B (en) | Breeding method of high-quality lodging-resistant long-grain japonica rice | |
| WO2022105011A1 (en) | Cultivation method based on led light source and used for promoting blossoming and fruiting of tomatoes | |
| CN104372034A (en) | Method for production of resveratrol from polygonum cuspidatum trichoid root and enlarged cultivation | |
| CN115644066B (en) | Method for improving growth quantity and flavonoid content of longan embryo callus by using exogenous polyamine | |
| CN1328983A (en) | Amino acid composite liquid fertilizer | |
| CN107548990B (en) | Culture method for improving starch content of duckweed and application | |
| CN108323427B (en) | Method for improving duckweed starch yield by optimizing spectral composition | |
| CN103540538B (en) | Little song killer yeast sort of quyi and preparation method thereof | |
| CN105961200A (en) | Tobacco anther differential medium and preparation method | |
| CN1795757A (en) | Method for brewing soybean sauce | |
| CN105315695B (en) | A kind of agricultural film and its manufacture method | |
| CN108083447B (en) | Method for promoting duckweed to rapidly purify micro-polluted surface water by utilizing high-quality light source | |
| CN105794626A (en) | Method for promoting diffusion of altar laver shell protonema conchospore | |
| CN106086144A (en) | A kind of method utilizing photocatalytic effect induction microalgae astaxanthin accumulation | |
| CN102531782B (en) | Preparation method of growth regulator for quickly turfing lawn in low-illumination environment | |
| CN112931143B (en) | Seedling raising matrix special for mangoes and preparation method | |
| CN104663519A (en) | Cultivation method with high yield of freshwater silvery pomfret pond | |
| CN108863619A (en) | A kind of Rosa roxburghii Tratt selenium-rich liquid bacterial manure and the preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB03 | Change of inventor or designer information | ||
| CB03 | Change of inventor or designer information |
Inventor after: Zhao Hai Inventor after: Fang Yang Inventor after: Jin Yanling Inventor after: He Kaize Inventor after: Xu Yaliang Inventor after: Li Jinmeng Inventor after: Yang Guili Inventor before: Zhao Hai Inventor before: Fang Yang Inventor before: Jin Yanling Inventor before: He Kaize Inventor before: Xu Yaliang Inventor before: Yang Guili |
|
| GR01 | Patent grant | ||
| GR01 | Patent grant |