CN102531782B - Preparation method of growth regulator for quickly turfing lawn in low-illumination environment - Google Patents

Preparation method of growth regulator for quickly turfing lawn in low-illumination environment Download PDF

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CN102531782B
CN102531782B CN 201110457086 CN201110457086A CN102531782B CN 102531782 B CN102531782 B CN 102531782B CN 201110457086 CN201110457086 CN 201110457086 CN 201110457086 A CN201110457086 A CN 201110457086A CN 102531782 B CN102531782 B CN 102531782B
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growth
lawn
preparation
growth regulator
liquid
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CN102531782A (en
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安渊
周鹏
任晓燕
高小燕
马力
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Shanghai Jiaotong University
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Abstract

The invention discloses a preparation method of a growth regulator for quickly turfing a lawn in a low-illumination environment. The preparation method comprises the following steps of: preparing a growth promoting fluid, a plant nutrient solution and a carbon source nutrient solution; mixing 1-2ml of growth promoting fluid, 1-2ml of plant nutrient solution and 8-10ml of carbon source nutrient solution; and mixing and fixing the volume with clear water to 1L to form the growth regulator for quickly turfing the lawn in the low-illumination environment. The prepared growth regulator plays rolesin increasing the photosynthetic efficiency of the lawn in a weak illumination environment, promoting tillering and root growth of the lawn and increasing the turfing speed of the lawn. Under the condition that the greenhouse illumination intensity is 15,000-25,000 lx, the regulator is sprayed onto a festuca elata lawn of which the average plant height is 5 centimeters, so that the plant height of the festuca elata lawn is increased by 8.6-13.2 percent in comparison to that of a control group (in which only clear water is sprayed), the root biomass is increased by 0.9-1.6 times in comparison to that of the control group, and the tillering time is 15-25 days earlier than that of the control group.

Description

Low light is according to the growth regulator preparation method on fast rapid-result level ground, lawn under the environment
Technical field
What the present invention relates to is the plant-growth regulator in a kind of gardens technology field, specifically is that a kind of low light is according to the preparation method of the growth regulator on fast rapid-result level ground, lawn under the environment.
Background technology
Land for growing field crops planting lawn and production are the principal modes that the present lawn of China produces, and the turf of urban afforestation more than 90% is provided, and important effect has been brought into play in urban afforestation.But strip vegetation continually, havoc agricultural land soil arable layer quality, influenced the sustainable use in farmland.Batch production production lawn is to utilize agricultural wastes as matrix, and multilayer level ground bed is set in the greenhouse, carries out a kind of advanced person's on stereo planting lawn method of planting lawn, has saved the farmland effectively, has protected the arable land.China's lawn selling price is very low, and average price is 2.5-3.5 unit/m 2, profit is very low.In order to reduce cost, batch production lawn, greenhouse production facility can not adopt the light irradiation apparatus that is similar to the facilities vegetable plantation, therefore, the illumination deficiency is the restraining factors that the batch production lawn produces, outstanding behaviours exists: 1) cause the lawn root growth slow, can not be woven into fast netted, the turf that formation can be curled; 2) turfgrass normal physiological processes and Nutrition and Metabolism are suppressed, and photosynthetic efficiency descends, and makes the lawn be in underfed situation, influences turfgrass normal growth and turf quality.
Under the situation at batch production lawn production illumination deficiency, how to promote low light according to lawn healthy growth under the intensity, the research on fast rapid-result level ground report is fewer, does not still have the otherwise effective technique measure at present.Find that through the literature search to prior art Chen Yu etc. (2006) have reported under the environment that shades that the annual bluegrass endogenous gibberellins increases, and has promoted the elongation growth of annual bluegrass stem in the survey article of " Chinese meadow journal " the 28th volume 3 phases (71-76 page or leaf); Xing Yuan and model magnificence (2010) be the 37th volume in " Anhui agricultural sciences, ", and the 8th phase (3522-3523 page or leaf) has been reported ordinary light according under the condition, and 0.01 μ g/L α-Nai Yisuan (NAA) has obviously promoted the growth of wheat root and bud.But under the situation of illumination deficiency, promote plant-growth regulator and the plant nutrient of plant-growth to improve the turfgrass root growth by using, improve the research report of photosynthetic efficiency and do not find as yet, also do not find a kind of method that possesses the above-mentioned functions characteristics.
Summary of the invention
The present invention is directed to the deficiency and the problem that exist in the background technology, the growth regulator preparation method of a kind of low light according to fast rapid-result level ground, lawn under the environment is provided.The growth regulator of the inventive method preparation can increase plant tillering effectively under the environment of greenhouse illumination deficiency, promote root growth, improves the lawn photosynthetic efficiency, accelerates the lawn turf speed, improves into the level ground quality.
The present invention is achieved by the following technical solutions:
Growth regulator of the present invention promotes liquid by growth, and plant nutrition liquid, carbon source nutritive medium and clear water mix to be formed, contain growth in every liter of this growth regulator and promote liquid 1-2ml, and plant nutrition liquid 1-2ml, carbon source nutritive medium 8-10ml, clear water are surplus; Wherein: described growth promotes liquid to be made up of Plant hormones regulators,gibberellins GA and α-Nai Yisuan NAA; Described plant nutrition liquid is made up of zinc Zn, magnesium Mg and manganese Mn; Described carbon source nutritive medium is made up of succsinic acid and citric acid;
Above-mentioned growth regulator prepares by following steps:
1) growth promotes the preparation of liquid:
(1) takes by weighing α-Nai Yisuan (NAA), be dissolved in ethanol until dissolving fully;
(2) take by weighing Plant hormones regulators,gibberellins (GA), be dissolved in distilled water;
(3) NAA and the GA solution with described (1) and (2) preparation mixes, and is settled to 1 liter (L) with clear water, forms described growth and promotes liquid;
2) preparation of plant nutrition liquid:
(1) takes by weighing the ZnSO that contains zinc (Zn) respectively 4The MgSO that contains magnesium (Mg) 4The MnCl that contains manganese (Mn) 2
(2) with the ZnSO in described (1) 4, MgSO 4And MnCl 2Be dissolved in fully in the 1L clear water, form described plant nutrition liquid.
3) preparation of carbon source nutritive medium:
(1) gets succsinic acid and citric acid respectively;
(2) succsinic acid in described (1) and citric acid are mixed, be settled to 1L with clear water, form described carbon source nutritive medium.
4) growth regulator preparation: getting growth respectively and promoting liquid, plant nutrition liquid and carbon source nutritive medium, mixing and be settled to 1L with clear water, forming a kind of low light according to the growth regulator on fast rapid-result level ground, lawn under the environment.
Further, described step 1) is specially:
(1) takes by weighing the pure product α-Nai Yisuan (NAA) (or quite non-pure product α-Nai Yisuan of the content) of 1.5g, be dissolved in a small amount of 95% ethanol until dissolving fully;
(2) take by weighing the pure product Plant hormones regulators,gibberellins (GA) (or quite non-pure product Plant hormones regulators,gibberellins of the content) of 50g, be dissolved in 100ml distilled water;
(3) NAA and the GA solution with described (1) and (2) preparation mixes, and is settled to 1 liter (L) with clear water, forms described growth and promotes liquid, and every liter of this growth promotes to contain GA:144mmol/L and NAA:8mmol/L respectively in the liquid.
Further, described step 2), be specially:
(1) takes by weighing the ZnSO that contains 65g zinc (Zn) respectively 4169g; The MgSO that contains 96g magnesium (Mg) 4512g; The MnCl that contains 55g manganese (Mn) 2126g;
(2) with the ZnSO in described (1) 4, MgSO 4And MnCl 2Be dissolved in fully in the 1L clear water, form described plant nutrition liquid, contain Zn:1mol/L, Mg:4mol/L and Mn:1mol/L respectively in every liter of this plant nutrition liquid.
Further, described step 3) is specially:
(1) gets the pure succsinic acid of 1.181g (or non-pure product succsinic acid of suitable content) and the pure citric acid of 1.921g (or non-pure product citric acid of suitable content) respectively;
(2) succsinic acid in described (1) and citric acid are mixed, be settled to 1L with clear water, form described carbon source nutritive medium, contain succsinic acid: 10mmol/L and citric acid: 10mmol/L respectively in every liter of this carbon source nutritive medium.
Further, described step 4) is specially:
Get the 1-2ml growth respectively and promote liquid, 1-2ml plant nutrition liquid and 8-10ml carbon source nutritive medium, mix and be settled to 1L with clear water, form a kind of low light according to the growth regulator on fast rapid-result level ground, lawn under the environment, wherein, contain Plant hormones regulators,gibberellins in this growth regulator of every 1L: 144-288 μ mol/L, α-Nai Yisuan: 8-16 μ mol/L, Zn:1-2mmol/L, Mg:4-8mmol/L, Mn:1-2mmol/L, succsinic acid 80-100: μ mol/L and citric acid: 80-100 μ mol/L.
Fast rapid-result level ground, the lawn growth regulator that adopts aforesaid method of the present invention to obtain has the low light level of raising according to environment lawn photosynthetic efficiency, and increase is tillered, and promotes root growth, accelerates the effect of lawn Cheng Ping.Be under the condition of 15000-250001x in the greenhouse intensity of illumination, utilize this conditioning agent to spray average plant height and be the tall fescue turf of 5cm, sprayed 1 time every 10 days, spray altogether 3 times, spray 1-2L/m at every turn 2, to handle 30-40 days, the tall fescue turf plant height increases 8.6%-13.2% than contrast (only spraying clear water), and root biomass increases 0.9-1.6 doubly than contrast, and turf grass growing time has been done sth. in advance 15-25 days than contrast.
Embodiment
Below embodiments of the invention are elaborated: following examples are that prerequisite is implemented with the technical solution of the present invention, provided detailed embodiment and process, but protection scope of the present invention are not limited to following embodiment.
Embodiment one:
1, growth promotes the preparation of liquid:
(1) takes by weighing the pure product α-Nai Yisuan (NAA) of 1.5g, be dissolved in a small amount of 95% ethanol until dissolving fully;
(2) take by weighing the pure product Plant hormones regulators,gibberellins (GA) of 50g, be dissolved in 100ml distilled water;
(3) NAA and the GA solution with described (1) and (2) preparation mixes, and is settled to 1 liter (L) with clear water, forms described growth and promotes liquid, and every liter of this growth promotes to contain GA:144mmol/L and NAA:8mmol/L respectively in the liquid.
2, the preparation of plant nutrition liquid:
(1) takes by weighing the ZnSO that contains 65g zinc (Zn) respectively 4169g; The MgSO that contains 96g magnesium (Mg) 4512g; The MnCl that contains 55g manganese (Mn) 2126g;
(2) with the ZnSO in described (1) 4, MgSO 4And MnCl 2Be dissolved in fully in the 1L clear water, form described plant nutrition liquid, contain Zn:1mol/L, Mg:4mol/L and Mn:1mol/L respectively in every liter of this plant nutrition liquid.
3, the preparation of carbon source nutritive medium:
(1) gets the pure succsinic acid of 1.181g and the pure citric acid of 1.921g respectively;
(2) succsinic acid in described (1) and citric acid are mixed, be settled to 1L with clear water, form described carbon source nutritive medium, contain succsinic acid: 10mmol/L and citric acid: 10mmol/L respectively in every liter of this carbon source nutritive medium.
4, low light prepares according to the growth regulator on fast rapid-result level ground, lawn under the environment:
Get the 1ml growth respectively and promote liquid, 1ml plant nutrition liquid and 8ml carbon source nutritive medium, be settled to 1L with its mixing and with clear water, form a kind of low light according to the growth regulator on fast rapid-result level ground, lawn under the environment, wherein, contain Plant hormones regulators,gibberellins in every liter of this growth regulator: 144 μ mol/L, α-Nai Yisuan: 8mol/L, Zn:1mmol/L, Mg:4mmol/L, Mn:1mmol/L, succsinic acid 80 μ mol/L and citric acids: 80 μ mol/L.
Under the 25000lx photoenvironment, contrast and two processing of this growth regulator are set respectively, the lawn becomes the level ground effect to see Table 1.
It is the tall fescue turf of 5cm that described control treatment adopts the average plant height of fresh water spraying, sprays 1 time every 10 days, sprays altogether 3 times, sprays 1-2L/m at every turn 2
Described growth regulator handled the growth regulator that the low light that adopts the embodiment of the invention one preparation shines fast rapid-result level ground, lawn under the environment, sprays the tall fescue turf that average plant height is 5cm, sprays 1 time every 10 days, sprays altogether 3 times, sprays 1-2L/m at every turn 2
The lawn of table 1 embodiment one becomes the level ground effect
Figure BDA0000127483390000051
Embodiment two
1, growth promotes the preparation of liquid:
(1) takes by weighing non-pure product α-Nai Yisuan with the suitable content of pure product α-Nai Yisuan of 1.5g, be dissolved in a small amount of 95% ethanol until dissolving fully;
(2) take by weighing non-pure product Plant hormones regulators,gibberellins with the suitable content of pure product Plant hormones regulators,gibberellins of 50g, be dissolved in 100ml distilled water;
(3) NAA and the GA solution with described (1) and (2) preparation mixes, and is settled to 1 liter (L) with clear water, forms described growth and promotes liquid, and every liter of this growth promotes to contain GA:144mmol/L and NAA:8mmol/L respectively in the liquid.
2, the preparation of plant nutrition liquid:
(1) takes by weighing the ZnSO that contains 65g zinc (Zn) respectively 4169g; The MgSO that contains 96g magnesium (Mg) 4512g; The MnCl that contains 55g manganese (Mn) 2126g;
(2) with the ZnSO in described (1) 4, MgSO 4And MnCl 2Be dissolved in fully in the 1L clear water, form described plant nutrition liquid, contain Zn:1mol/L, Mg:4mol/L and Mn:1mol/L respectively in every liter of this plant nutrition liquid.
3, the preparation of carbon source nutritive medium:
(1) gets non-pure product succsinic acid with the suitable content of the pure succsinic acid of 1.181g respectively, with the non-pure product citric acid of the suitable content of the pure citric acid of 1.921g;
(2) succsinic acid in described (1) and citric acid are mixed, be settled to 1L with clear water, form described carbon source nutritive medium, contain succsinic acid: 10mmol/L and citric acid: 10mmol/L respectively in every liter of this carbon source nutritive medium.
4, low light prepares according to the growth regulator on fast rapid-result level ground, lawn under the environment: get the 1.5ml growth respectively and promote liquid, 1.5ml plant nutrition liquid and 9ml carbon source nutritive medium, be settled to 1L with its mixing and with clear water, form a kind of low light according to the growth regulator on fast rapid-result level ground, lawn under the environment, wherein, contain Plant hormones regulators,gibberellins in every liter of this growth regulator: 226 μ mol/L, α-naphthylacetic acid: 12 μ mol/L, Zn:1.5mmol/L, Mg:6mmol/L, Mn:1.5mmol/L, succsinic acid 90: μ mol/L and citric acid: 90 μ mol/L.
Under the 20000lx photoenvironment, contrast and two processing of this growth regulator are set respectively, the lawn becomes the level ground effect to see Table 2.
It is the tall fescue turf of 5cm that described control treatment adopts the average plant height of fresh water spraying, sprays 1 time every 10 days, sprays altogether 3 times;
Described growth regulator handled the growth regulator that a kind of low light that adopts the embodiment of the invention two preparations shines fast rapid-result level ground, lawn under the environment, sprays the tall fescue turf that average plant height is 5cm, sprays 1 time every 10 days, sprays altogether 3 times.
The lawn of table 2 embodiment two becomes the level ground effect
Figure BDA0000127483390000061
Embodiment three
1, growth promotes the preparation of liquid: according to the described method preparation of embodiment 1 step 1);
2, the preparation of plant nutrition liquid: according to embodiment 1 step 2) described method preparation;
3, the preparation of carbon source nutritive medium: according to the described method preparation of embodiment 1 step 3);
4, low light prepares according to the growth regulator on fast rapid-result level ground, lawn under the environment: get the 2ml growth respectively and promote liquid, 2ml plant nutrition liquid and 10ml carbon source nutritive medium, be settled to 1L with its mixing and with clear water, form a kind of low light according to the growth regulator on fast rapid-result level ground, lawn under the environment, wherein, contain Plant hormones regulators,gibberellins in every liter of this growth regulator: 288 μ mol/L, α-Nai Yisuan: 16 μ mol/L, Zn:2mmol/L, Mg:8mmol/L, Mn:2mmol/L, succsinic acid: 100 μ mol/L and citric acids: 100 μ mol/L.
Under the 15000lx photoenvironment, contrast and two processing of this growth regulator are set respectively, the lawn becomes the level ground effect to see Table 3.
It is the tall fescue turf of 5cm that described control treatment adopts the average plant height of fresh water spraying, sprays 1 time every 10 days, sprays altogether 3 times;
Described growth regulator handled the growth regulator that a kind of low light that adopts the embodiment of the invention three preparations shines fast rapid-result level ground, lawn under the environment, sprays the tall fescue turf that average plant height is 5cm, sprays 1 time every 10 days, sprays altogether 3 times.
The lawn of table 3 embodiment three becomes the level ground effect
Figure BDA0000127483390000071
What more than provide is the preferred embodiments of the present invention, and the present invention also has other embodiment, and such as the conversion content range etc. of material wherein, these are easy to realize for a person skilled in the art.
Although content of the present invention has been done detailed introduction by above preferred embodiment, will be appreciated that above-mentioned description should not be considered to limitation of the present invention.After those skilled in the art have read foregoing, for multiple modification of the present invention with to substitute all will be apparent.Therefore, protection scope of the present invention should be limited to the appended claims.

Claims (4)

1. a low light is according to the growth regulator preparation method on fast rapid-result level ground, lawn under the environment, it is characterized in that, described growth regulator promotes liquid by growth, plant nutrition liquid, carbon source nutritive medium and clear water mix to be formed, and contains growth in every liter of this growth regulator and promotes liquid 1-2ml, plant nutrition liquid 1-2ml, carbon source nutritive medium 8-10ml, clear water are surplus; Wherein: described growth promotes liquid to be made up of Plant hormones regulators,gibberellins GA and α-Nai Yisuan NAA; Described plant nutrition liquid is made up of zinc Zn, magnesium Mg and manganese Mn; Described carbon source nutritive medium is made up of succsinic acid and citric acid; This growth regulator prepares by following steps:
1) growth promotes the preparation of liquid:
(1) takes by weighing α-Nai Yisuan NAA, be dissolved in ethanol until dissolving fully;
(2) take by weighing Plant hormones regulators,gibberellins GA, be dissolved in distilled water;
(3) NAA and the GA solution with described (1) and (2) preparation mixes, and is settled to 1L with clear water, forms described growth and promotes liquid;
2) preparation of plant nutrition liquid:
(1) takes by weighing the ZnSO that contains zinc respectively 4The MgSO that contains magnesium 4The MnCl that contains manganese 2
(2) with the ZnSO in described (1) 4, MgSO 4And MnCl 2Be dissolved in fully in the 1L clear water, form described plant nutrition liquid;
3) preparation of carbon source nutritive medium:
(1) gets succsinic acid and citric acid respectively;
(2) succsinic acid in described (1) and citric acid are mixed, be settled to 1L with clear water, form described carbon source nutritive medium;
4) growth regulator preparation: get growth respectively and promote liquid, plant nutrition liquid and carbon source nutritive medium, mix and be settled to 1L with clear water, form a kind of low light according to the growth regulator on fast rapid-result level ground, lawn under the environment, contain Plant hormones regulators,gibberellins in this growth regulator of every 1L: 144-288 μ mol/L, α-Nai Yisuan: 8-16 μ mol/L, Zn:1-2mmol/L, Mg:4-8mmol/L, Mn:1-2mmol/L, succsinic acid: 80-100 μ mol/L and citric acid: 80-100 μ mol/L.
2. low light according to claim 1 is characterized in that according to the growth regulator preparation method on fast rapid-result level ground, lawn under the environment described step 1) is specially:
(1) takes by weighing the pure product α-Nai Yisuan NAA of 1.5g or the suitable non-pure product α-Nai Yisuan of content, be dissolved in a small amount of 95% ethanol until dissolving fully;
(2) take by weighing the pure product Plant hormones regulators,gibberellins GA of 50g or the non-pure product Plant hormones regulators,gibberellins of suitable content, be dissolved in 100ml distilled water;
(3) NAA and the GA solution with described (1) and (2) preparation mixes, and is settled to 1L with clear water, forms described growth and promotes liquid, and every liter of this growth promotes to contain GA:144mmol/L and NAA:8mmol/L respectively in the liquid.
3. low light according to claim 1 is characterized in that described step 2 according to the growth regulator preparation method on fast rapid-result level ground, lawn under the environment), be specially:
(1) takes by weighing the ZnSO that contains 65g zinc respectively 4169g; The MgSO that contains 96g magnesium 4512g; The MnCl that contains 55g manganese 2126g;
(2) with the ZnSO in described (1) 4, MgSO 4And MnCl 2Be dissolved in fully in the 1L clear water, form described plant nutrition liquid, contain Zn:1mol/L, Mg:4mol/L and Mn:1mol/L respectively in every liter of this plant nutrition liquid.
4. low light according to claim 1 is characterized in that according to the growth regulator preparation method on fast rapid-result level ground, lawn under the environment described step 3) is specially:
(1) gets the non-pure product succsinic acid of the pure succsinic acid of 1.181g or suitable content and the non-pure product citric acid of the pure citric acid of 1.921g or suitable content respectively;
(2) succsinic acid in described (1) and citric acid are mixed, be settled to 1L with clear water, form described carbon source nutritive medium, contain succsinic acid: 10mmol/L and citric acid: 10mmol/L respectively in every liter of this carbon source nutritive medium.
CN 201110457086 2011-12-30 2011-12-30 Preparation method of growth regulator for quickly turfing lawn in low-illumination environment Expired - Fee Related CN102531782B (en)

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CN106007953A (en) * 2016-05-20 2016-10-12 太仓市雅丰农场专业合作社 Aquatic plant growth regulating agent and preparation method thereof
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