CN107548982A - A kind of method by promoting hypopus to form raising duckweed total starch yield - Google Patents
A kind of method by promoting hypopus to form raising duckweed total starch yield Download PDFInfo
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Abstract
The invention belongs to aquatic energy-source plant production field, provide a kind of by promoting hypopus to form the method for improving duckweed total starch yield, duckweed is inoculated in water body by this method, cultivated 14~30 days under the conditions of the irradiation of feux rouges, blue light or red blue mixed light, harvest duckweed hypopus and thallus.This method can improve production efficiency cost-effective while, be effectively increased duckweed total starch yield, and not limited by water nutrition situation, have general applicability.
Description
Technical field
It is more particularly to a kind of to improve duckweed by promoting hypopus to be formed the invention belongs to aquatic energy-source plant production field
The method of total starch yield.
Background technology
More duckweeds of Lemnaceae can produce one kind and be clearly distinguishable from normal thallophytic nutrition propagule, i.e. hypopus
(turion).Hypopus and common thallus are differentiated by identical phyllopodium, and the formation of hypopus means duckweed
Developmental condition changed.The most important feature of hypopus is that content of starch is higher, can generally reach 60% (dry weight)
More than, and corresponding thallophytic content of starch, generally 4%~10% or so, highest can only also reach under inductive condition
To 30%.The starch granules particle diameter of more duckweed hypopus is at 4 μm or so, relative to the starch granules particle diameter of corn, rice etc.
It is smaller, thus be easier to hydrolyze and produced for bio-fuel.Because the aerating tissue of hypopus is less, density is larger, therefore
It can separate, and sink under water with parent after maturation, be easy to separately harvest with the parent floated.Meanwhile compared with thallus, dormancy
Body have higher quality/area than with lower moisture content, therefore the drying process of hypopus can be more economical after harvesting.
These features cause more duckweed hypopus to become a kind of very promising bio-fuel raw materials for production.Although more duckweeds stop
The content of starch of dormancy body is high, but its dry substance accumulation speed is slow, yields poorly, and the starch yield of actually more duckweed hypopus is
Than relatively low, this into more duckweed hypopus bio-fuel production field practical application critical limiting factor.
At present, method caused by more duckweed hypopus is promoted to be divided into two classes.A kind of method is by promoting thallus
Photosynthesis increase thallus quantity, so as to increasing the quantity of hypopus, specifically include by added into water body sucrose,
Using high intensity of illumination culture and in high CO2Cultivated under concentration conditions, although this kind of method is quantitative in increase hypopus
Effect is more obvious, but operation is complex, and time-consuming longer, low production efficiency, production cost is higher, does not have economy.Separately
A kind of method mainly promotes the formation of hypopus by way of abiotic stress, specifically includes by nutritional deficiency, low
Temperature, the modes such as abscisic acid are sprayed, lifting effect of this kind of method to hypopus quantity is very limited, while can also suppress thallus
Growth, cause thallophytic starch yield to reduce, thus the total starch yield of actually more duckweeds is still very low.
The content of the invention
It is an object of the invention to overcome the deficiencies of the prior art and provide one kind duckweed is improved by promoting hypopus to be formed
The method of total starch yield, to improve duckweed total starch yield on the basis of improving production efficiency and reducing production cost.
Method provided by the invention by promoting hypopus to form raising duckweed total starch yield, it is to be inoculated in duckweed
In water body, cultivated 14~30 days under the conditions of the irradiation of feux rouges, blue light or red-blue mixed light, harvest duckweed hypopus and leaf
Shape body.
In the above method, according to the photon hypothesis (PPFD) of feux rouges, blue light or red-blue mixed light for 80~
200μmol/m2/ s illumination condition culture duckweed.
In the above method, the peak wavelength of feux rouges is 660nm, and the peak wavelength of blue light is 450nm, the half of feux rouges and blue light
High peak width is narrower, and the method for the invention is better to the raising effect of duckweed total starch yield, it is preferable that the peak width at half height of feux rouges
No more than 15nm, the peak width at half height of blue light is no more than 20nm.
In the above method, when mixing light irradiation using red-blue, the light quantum of feux rouges and blue light in red-blue mixed light leads to
The ratio between metric density is 1:(0.25~1).
In the above method, using red LED lamp provide feux rouges, using blue LED lamp provide blue light, by red LED lamp with
Blue LED lamp combination provides red-blue mixed light.
In the above method, in order to improve duckweed total starch yield to greatest extent, preferably covered according to 80%~100%
Lid rate is by duckweed kind in water body.
In the above method, the duckweed is more duckweeds, particularly more duckweed gx1.
Compared with prior art, the invention has the advantages that：
1. the invention provides a kind of by promoting hypopus to form the method for improving duckweed total starch yield, this method is led to
The developmental condition that monochromatic feux rouges or blue LED lamp either red-blue mixed light LED are crossed as light source induction duckweed becomes
Change, while improve its photosynthetic efficiency, more duckweed hypopus starch yields and duckweed total starch yield can be increased substantially, simultaneously
The light source that the present invention uses can reduce power consumption compared with common white light, the side with existing increase duckweed hypopus quantity
Method is compared, and method of the invention can improve production efficiency cost-effective while, be effectively increased duckweed total starch yield.
2. experiment shows, more duckweeds, more duckweed dormancy are cultivated under the conditions of eutrophy using the method for the invention
Body and thallophytic starch accumulation speed are obtained for raising, and its total starch accumulating rate has reached 1.41~2.34g/m2/ d,
Apparently higher than the total starch accumulating rate 0.49g/m under corresponding white light illumination condition2/ d, more purples are cultivated under the conditions of oligotrophic
Duckweed, more duckweed hypopus and thallophytic starch accumulation speed are also improved simultaneously, and its total starch accumulating rate reaches
3.04~4.18g/m2/ d, hence it is evident that higher than the total starch accumulating rate 2.5g/m under corresponding white light illumination condition2/ d, illustrate this
The method of invention can effectively improve the starch yield of more duckweeds without being limited by water nutrition situation, have and be generally applicable
Property.
3. it is 110 μm of ol/m in photon hypothesis2Duckweed is cultivated under conditions of/s, with making using white LED lamp
Compared for light source, the method for the invention is using monochromatic feux rouges or blue LED lamp either red-blue mixed light LED as light
Source, power consumption 12.56% can be at most reduced while hypopus starch yield and duckweed total starch yield is increased, there is section
The about advantage of illumination cost.
Brief description of the drawings
Fig. 1 is the dry substance accumulation speed comparison diagram of hypopus in comparative example 1 and embodiment 1~6.
Fig. 2 is thallophytic dry substance accumulation speed comparison diagram in comparative example 1 and embodiment 1~6.
Fig. 3 is the content of starch comparison diagram of hypopus in comparative example 1 and embodiment 1~6.
Fig. 4 is thallophytic content of starch comparison diagram in comparative example 1 and embodiment 1~6.
Fig. 5 is total starch accumulating rate comparison diagram in comparative example 1 and embodiment 1~6.
Fig. 6 is the dry substance accumulation speed comparison diagram of hypopus in comparative example 2 and embodiment 7~12.
Fig. 7 is thallophytic dry substance accumulation speed comparison diagram in comparative example 2 and embodiment 7~12.
Fig. 8 is the content of starch comparison diagram of hypopus in comparative example 2 and embodiment 7~12.
Fig. 9 is thallophytic content of starch comparison diagram in comparative example 2 and embodiment 7~12.
Figure 10 is total starch accumulating rate comparison diagram in comparative example 2 and embodiment 7~12.
Embodiment
Below by embodiment and comparative example the production of duckweed total starch is improved to of the present invention by promoting hypopus to be formed
The method of amount is described further.
In following each embodiments and comparative example, the assay method of duckweed content of starch can be found in Zhang L., Chen Q.,
Jin Y.,et al.Energy-saving direct ethanol production from viscosity reduction
mash of sweet potato at very high gravity(VHG).Fuel Processing
Technology.2010,91(12):1845-1850.Specifically assay method is：Respectively by dry Duckweed fronds and dormancy
Body is ground into powder, weighs 0.03~0.06g duckweed dry powder and is placed in 250mL ground conical flasks, adds 30mL 6mol/L's
HCl solution and 100mL distilled water, load onto condenser pipe, put the 2h that flowed back in boiling water bath.Backflow finishes, and immediately with flowing water cooling, treats
After duckweed sample hydrolyzate is cooled to room temperature, the pH value for adding NaOH regulation hydrolyzates is 7.Then 20mL 20wt% vinegar is added
Lead plumbate solution, 10min is placed after shaking up, be transferred in 500mL volumetric flasks, add distilled water to be settled to 500mL, filtered, discarded just
Filtrate, collect 5mL filtrates and cross the good anti-phase C18 solid phase extraction columns of pre-activate, discard 1 initial~2mL, collect 3 below
~4mL, then with 0.22 μm of water system membrane filtration.Glucose content in filtrate is determined using HPLC, according to content of starch=Portugal
Grape sugared content/1.1, calculate the content of starch in Duckweed fronds and hypopus.
In following each embodiments, the compound method of various solution is as follows：
The compound method of Hoagland nutrient solutions is as follows：(1) with distilled water according to respectively being tried in the mother liquor formula described in following table
The concentration of agent prepares six kinds of mother liquors of A, B, C, D, E, F, wherein, when preparing mother liquor A, first with 6N HCl solution by Ca (NO3)2·
4H2O、KNO3、KH2PO4Dissolving, then add distilled water and be configured to aimed concn；When preparing mother liquor D, first with 6N KOH solution
EDTA is dissolved, distilled water is then added and is configured to aimed concn.(2) according in every liter of Hoagland nutrient solution described in following table
The addition of various mother liquors prepares Hoagland nutrient solutions with distilled water, and Hoagland nutrient solutions are adjusted by HCl and NaOH
PH value is 5.0.In the Hoagland nutrient solutions prepared, the concentration of N element is 349.73mg/L, and the concentration of P element is
154.89mg/L。
The compound method of 1/5 concentration Hoagland nutrient solutions：By a certain amount of Hoagland nutrient solutions and 4 times of its volumes
Distilled water well mixed produce.
The duckweed used in following each embodiments and comparative example is more duckweed gx1, gathers and is saved from Guangxi China.
For common white LED lamp, its light quality composition is the light source used in following each comparative examples：Blue light/green/red
(B/G/R)=37/45/28, the ratio is the ratio between photon hypothesis of blue and green light and feux rouges.In following each embodiments,
Red LED lamp, blue LED lamp offer feux rouges and blue light are provided, red LED lamp is combined into red-blue with blue LED lamp and mixed
Closing light LED provides red-blue mixed light.The peak wavelength of the feux rouges is 660nm, peak width at half height 15nm, the peak value of blue light
Wavelength is 450nm, peak width at half height 20nm.When red-blue mixed light is provided, the different application demand in each embodiment, point
Combined not by red LED lamp with blue LED lamp and to form the photon hypothesis ratio of feux rouges and blue light and be respectively：Feux rouges/blue light
(R/B)=1/2,1/1,2/1 and 4/1 red-blue mixed light LED.
It is 110 μm of ol/m according to photon hypothesis in following each comparative examples and embodiment2/ s full the light of illumination condition
According to culture duckweed, photon hypothesis therein each means that corresponding light source is close in the light for the water surface for being vaccinated with duckweed
Degree.
Comparative example 1：Common white LED lamp+eutrophy CMC model
Duckweed is inoculated in 250mL 1/5Hoagland nutrient solutions by 100% coverage, 1/5Hoagland nutrient solutions
In total nitrogen concentration be 70mg/L, total phosphorus concentration 30mg/L, using common white LED lamp as light source, at 25 DEG C according to light
Quantum flux density is 110 μm of ol/m2/ s illumination condition full exposure culture, daily with the water that distilled water supplement evaporates to original
Liquid level, cultivate 14 days, harvest thallus and hypopus respectively.
By the thallus of harvest and hypopus respectively with after distilled water flushing three times, it is placed in filter bag and is sloughed certainly through drier
By water, constant weight is dried to overnight at 60 DEG C in baking oven, and record of weighing, calculate thallus and the dry-matter accumulation speed of hypopus
Degree.By dried sample mill powdering and claim to survey glucose content with HPLC methods after acidolysis in right amount, calculate content of starch.Knot
Fruit shows that the average dry substance accumulation speed of hypopus is 0.32g/m2/ d, hypopus content of starch are 69.49%, lobate soma
Material accumulating rate is 5.76g/m2/ d, thallus content of starch are 4.82%, and duckweed total starch accumulating rate is 0.49g/m2/
d。
Embodiment 1：Blue LED lamp+eutrophy CMC model
Duckweed is inoculated in 250mL 1/5Hoagland nutrient solutions by 100% coverage, 1/5Hoagland nutrient solutions
In total nitrogen concentration be 70mg/L, total phosphorus concentration 30mg/L, using blue LED lamp as light source, at 25 DEG C according to light quantum
Flux density is 110 μm of ol/m2/ s illumination condition full exposure culture, daily with the water that distilled water supplement evaporates to stoste face
Highly, cultivate 14 days, harvest thallus and hypopus respectively.
Using determining and calculate the thallus that harvests and hypopus in the present embodiment with the identical experiment condition of comparative example 1
Dry substance accumulation speed and content of starch, as a result show, the average dry substance accumulation speed of hypopus is 1.02g/m2/ d, dormancy
Body content of starch is 72.00%, and thallus dry substance accumulation speed is 6.09g/m2/ d, thallus content of starch are 10.94%,
Duckweed total starch accumulating rate is 1.49g/m2/d。
Embodiment 2：Red LED lamp+eutrophy CMC model
Duckweed is inoculated in 250mL 1/5Hoagland nutrient solutions by 100% coverage, 1/5Hoagland nutrient solutions
In total nitrogen concentration be 70mg/L, total phosphorus concentration 30mg/L, using red LED lamp as light source, at 25 DEG C according to light quantum
Flux density is 110 μm of ol/m2/ s illumination condition full exposure culture, daily with the water that distilled water supplement evaporates to stoste face
Highly, cultivate 14 days, harvest thallus and hypopus respectively.
Using determining and calculate the thallus that harvests and hypopus in the present embodiment with the identical experiment condition of comparative example 1
Dry substance accumulation speed and content of starch, as a result show, the average dry substance accumulation speed of hypopus is 2.01g/m2/ d, dormancy
Body content of starch is 73.35%, and thallus dry substance accumulation speed is 5.99g/m2/ d, thallus content of starch are 7.32%,
Duckweed total starch accumulating rate is 1.94g/m2/d。
Embodiment 3：R/B:1/2 mixed light LED+eutrophy CMC model
Duckweed is inoculated in 250mL 1/5Hoagland nutrient solutions by 100% coverage, 1/5Hoagland nutrient solutions
In total nitrogen concentration be 70mg/L, total phosphorus concentration 30mg/L, use the photon hypothesis of feux rouges and blue light ratio as 1:2
Red-blue mixed light LED according to photon hypothesis is 110 μm of ol/m at 25 DEG C as light source2/ s full the light of illumination condition
According to culture, daily with the water that distilled water supplement evaporates to former liquid level, cultivate 14 days, harvest thallus and dormancy respectively
Body.
Using determining and calculate the thallus that harvests and hypopus in the present embodiment with the identical experiment condition of comparative example 1
Dry substance accumulation speed and content of starch, as a result show, the average dry substance accumulation speed of hypopus is 1.39g/m2/ d, dormancy
Body content of starch is 75.04%, and thallus dry substance accumulation speed is 7.21g/m2/ d, thallus content of starch are 8.09%,
Duckweed total starch accumulating rate is 1.68g/m2/d。
Embodiment 4：R/B:1/1 mixed light LED+eutrophy CMC model
Duckweed is inoculated in 250mL 1/5Hoagland nutrient solutions by 100% coverage, 1/5Hoagland nutrient solutions
In total nitrogen concentration be 70mg/L, total phosphorus concentration 30mg/L, use the photon hypothesis of feux rouges and blue light ratio as 1:1
Red-blue mixed light LED according to photon hypothesis is 110 μm of ol/m at 25 DEG C as light source2/ s full the light of illumination condition
According to culture, daily with the water that distilled water supplement evaporates to former liquid level, cultivate 14 days, harvest thallus and dormancy respectively
Body.
Using determining and calculate the thallus that harvests and hypopus in the present embodiment with the identical experiment condition of comparative example 1
Dry substance accumulation speed and content of starch, as a result show, the average dry substance accumulation speed of hypopus is 0.98g/m2/ d, dormancy
Body content of starch is 75.54%, and thallus dry substance accumulation speed is 6.92g/m2/ d, thallus content of starch are 8.89%,
Duckweed total starch accumulating rate is 1.41g/m2/d。
Embodiment 5：R/B:2/1 mixed light LED+eutrophy CMC model
Duckweed is inoculated in 250mL 1/5Hoagland nutrient solutions by 100% coverage, 1/5Hoagland nutrient solutions
In total nitrogen concentration be 70mg/L, total phosphorus concentration 30mg/L, use the photon hypothesis of feux rouges and blue light ratio as 2:1
Red-blue mixed light LED according to photon hypothesis is 110 μm of ol/m at 25 DEG C as light source2/ s full the light of illumination condition
According to culture, daily with the water that distilled water supplement evaporates to former liquid level, cultivate 14 days, harvest thallus and dormancy respectively
Body.
Using determining and calculate the thallus that harvests and hypopus in the present embodiment with the identical experiment condition of comparative example 1
Dry substance accumulation speed and content of starch, as a result show, the average dry substance accumulation speed of hypopus is 2.17g/m2/ d, dormancy
Body content of starch is 71.12%, and thallus dry substance accumulation speed is 6.77g/m2/ d, thallus content of starch are 9.81%,
Duckweed total starch accumulating rate is 2.28g/m2/d。
Embodiment 6：R/B:4/1 mixed light LED+eutrophy CMC model
Duckweed is inoculated in 250mL 1/5Hoagland nutrient solutions by 100% coverage, 1/5Hoagland nutrient solutions
In total nitrogen concentration be 70mg/L, total phosphorus concentration 30mg/L, use the photon hypothesis of feux rouges and blue light ratio as 4:1
Red-blue mixed light LED according to photon hypothesis is 110 μm of ol/m at 25 DEG C as light source2/ s full the light of illumination condition
According to culture, daily with the water that distilled water supplement evaporates to former liquid level, cultivate 14 days, harvest thallus and dormancy respectively
Body.
Using determining and calculate the thallus that harvests and hypopus in the present embodiment with the identical experiment condition of comparative example 1
Dry substance accumulation speed and content of starch, as a result show, the average dry substance accumulation speed of hypopus is 2.09g/m2/ d, dormancy
Body content of starch is 74.92%, and thallus dry substance accumulation speed is 6.89g/m2/ d, thallus content of starch are 10.12%,
Duckweed total starch accumulating rate is 2.34g/m2/d。
From Fig. 1 and Fig. 3, using feux rouges, blue light and red-blue mixing light irradiation culture 14 under the conditions of eutrophy
My god, the average dry substance accumulation speed of hypopus has reached 0.98~2.17g/m2/ d, hypopus content of starch be 71.12%~
75.54%, and white light culture 14 days is used, the average dry substance accumulation speed of hypopus is only 0.32g/m2/ d, hypopus
Content of starch is 69.49%, illustrates that the method for the invention can effectively improve dormancy while hypopus content of starch improves
Body dry substance accumulation speed.More duckweeds are cultivated under the conditions of eutrophy using the method for the invention, dormancy can be significantly improved
The starch yield of body.
From Fig. 2 and Fig. 4, using feux rouges, blue light and red-blue mixing light irradiation culture 14 under the conditions of eutrophy
My god, the average dry substance accumulation speed of thallus is 5.99~7.21g/m2/ d, thallus content of starch be 7.32%~
10.94%, and white light culture 14 days is used, the average dry substance accumulation speed of thallus is 5.76g/m2/ d, thallus are formed sediment
Powder content is 4.82%, illustrates that the method for the invention can improve thallus content of starch and dry substance accumulation speed simultaneously.Say
It is bright that more duckweeds are cultivated under the conditions of eutrophy using the method for the invention, thallophytic starch yield can be improved.
As shown in Figure 5, using feux rouges, blue light and red-blue mixing light irradiation culture 14 days, more under the conditions of eutrophy
The total starch accumulating rate of duckweed has reached 1.41~2.34g/m2/ d, and use the total of 14 days more duckweeds of white light culture
Starch accumulation speed is only 0.49g/m2/ d, illustrate that method provided by the invention can effectively improve the total starch production of more duckweeds
Amount.
Comparative example 2：Common white LED lamp+oligotrophic CMC model
Duckweed is inoculated in 250mL running water by 100% coverage, using common white LED lamp as light source, 25
DEG C it is 110 μm of ol/m according to photon hypothesis2/ s illumination condition full exposure culture, evaporated daily with distilled water supplement
Water to former liquid level, cultivate 14 days, harvest thallus and hypopus respectively.
Using determining and calculate the thallus harvested in this comparative example and hypopus with the identical experiment condition of comparative example 1
Dry substance accumulation speed and content of starch, as a result show, the average dry substance accumulation speed of 14 days hypopus is 3.20g/m2/ d,
Hypopus content of starch is 66.29%, and thallus dry substance accumulation speed is 2.42g/m2/ d, thallus content of starch are
15.98%, duckweed total starch accumulating rate is 2.50g/m2/d。
Embodiment 7：Blue LED lamp+oligotrophic CMC model
Duckweed is inoculated in 250mL running water by 100% coverage, using blue LED lamp as light source, pressed at 25 DEG C
It is 110 μm of ol/m according to photon hypothesis2/ s illumination condition full exposure culture, the water evaporated daily with distilled water supplement
To former liquid level, cultivate 14 days, harvest thallus and hypopus respectively.
Using determining and calculate the thallus that harvests and hypopus in the present embodiment with the identical experiment condition of comparative example 1
Dry substance accumulation speed and content of starch, as a result show, the average dry substance accumulation speed of 14 days hypopus is 3.67g/m2/ d,
Hypopus content of starch is 71.54%, and thallus dry substance accumulation speed is 2.69g/m2/ d, thallus content of starch are
23.19%, duckweed total starch accumulating rate is 3.25g/m2/d。
Embodiment 8：Red LED lamp+oligotrophic CMC model
Duckweed is inoculated in 250mL running water by 100% coverage, using red LED lamp as light source, pressed at 25 DEG C
It is 110 μm of ol/m according to photon hypothesis2/ s illumination condition full exposure culture, the water evaporated daily with distilled water supplement
To former liquid level, cultivate 14 days, harvest thallus and hypopus respectively.
Using determining and calculate the thallus that harvests and hypopus in the present embodiment with the identical experiment condition of comparative example 1
Dry substance accumulation speed and content of starch, as a result show, the average dry substance accumulation speed of 14 days hypopus is 3.59g/m2/ d,
Hypopus content of starch is 74.06%, and thallus dry substance accumulation speed is 2.35g/m2/ d, thallus content of starch are
16.19%, duckweed total starch accumulating rate is 3.04g/m2/d。
Embodiment 9：R/B:1/2 mixed light LED+oligotrophic CMC model
Duckweed is inoculated in 250mL running water by 100% coverage, the light quantum flux using feux rouges and blue light is close
Degree is than being 1:2 red-blue mixed light LED according to photon hypothesis is 110 μm of ol/m at 25 DEG C as light source2/ s's
Illumination condition full exposure culture, cultivated 14 days to former liquid level with the water that distilled water supplement evaporates daily, harvest leaf respectively
Shape body and hypopus.
Using determining and calculate the thallus that harvests and hypopus in the present embodiment with the identical experiment condition of comparative example 1
Dry substance accumulation speed and content of starch, as a result show, the average dry substance accumulation speed of 14 days hypopus is 4.79g/m2/ d,
Hypopus content of starch is 76.51%, and thallus dry substance accumulation speed is 2.75g/m2/ d, thallus content of starch are
18.45%, duckweed total starch accumulating rate is 4.18g/m2/d。
Embodiment 10：R/B:1/1 mixed light LED+oligotrophic CMC model
Duckweed is inoculated in 250mL running water by 100% coverage, the light quantum flux using feux rouges and blue light is close
Degree is than being 1:1 red-blue mixed light LED according to photon hypothesis is 110 μm of ol/m at 25 DEG C as light source2/ s's
Illumination condition full exposure culture, cultivated 14 days to former liquid level with the water that distilled water supplement evaporates daily, harvest leaf respectively
Shape body and hypopus.
Using determining and calculate the thallus that harvests and hypopus in the present embodiment with the identical experiment condition of comparative example 1
Dry substance accumulation speed and content of starch, as a result show, the average dry substance accumulation speed of 14 days hypopus is 4.59g/m2/ d,
Hypopus content of starch is 71.64%, and thallus dry substance accumulation speed is 2.72g/m2/ d, thallus content of starch are
18.83%, duckweed total starch accumulating rate is 3.79g/m2/d。
Embodiment 11：R/B:2/1 mixed light LED+oligotrophic CMC model
Duckweed is inoculated in 250mL running water by 100% coverage, using feux rouges and the photon hypothesis of blue light
Than for 2:1 red-blue mixed light LED according to photon hypothesis is 110 μm of ol/m at 25 DEG C as light source2/ s light
According to condition full exposure culture, cultivated 14 days, harvested respectively lobate to former liquid level with the water that distilled water supplement evaporates daily
Body and hypopus.
Using determining and calculate the thallus that harvests and hypopus in the present embodiment with the identical experiment condition of comparative example 1
Dry substance accumulation speed and content of starch, as a result show, the average dry substance accumulation speed of 14 days hypopus is 4.52g/m2/ d,
Hypopus content of starch is 77.01%, and thallus dry substance accumulation speed is 2.77g/m2/ d, thallus content of starch are
19.79%, duckweed total starch accumulating rate is 4.03g/m2/d。
Embodiment 12：R/B:4/1 mixed light LED+oligotrophic CMC model
Duckweed is inoculated in 250mL running water by 100% coverage, the light quantum flux using feux rouges and blue light is close
Degree is than being 4:1 red-blue mixed light LED according to photon hypothesis is 110 μm of ol/m at 25 DEG C as light source2/ s's
Illumination condition full exposure culture, cultivated 14 days to former liquid level with the water that distilled water supplement evaporates daily, harvest leaf respectively
Shape body and hypopus.
Using determining and calculate the thallus that harvests and hypopus in the present embodiment with the identical experiment condition of comparative example 1
Dry substance accumulation speed and content of starch, as a result show, the average dry substance accumulation speed of 14 days hypopus is 4.38g/m2/ d,
Hypopus content of starch is 75.97%, and thallus dry substance accumulation speed is 2.61g/m2/ d, thallus content of starch are
18.41%, duckweed total starch accumulating rate is 3.80g/m2/d。
From Fig. 6 and Fig. 8, using feux rouges, blue light and red-blue mixing light irradiation culture 14 under the conditions of oligotrophic
My god, the average dry substance accumulation speed of hypopus is 3.59~4.79g/m2/ d, hypopus content of starch be 71.54%~
77.01%, and white light culture 14 days is used, the average dry substance accumulation speed of hypopus is only 3.2g/m2/ d, hypopus form sediment
Powder content is 66.29%, and the method for the invention can improve hypopus content of starch and dry substance accumulation speed simultaneously.Using this
Invention methods described cultivates more duckweeds under the conditions of oligotrophic, can effectively improve the starch yield of hypopus.
From Fig. 7 and Fig. 9, using feux rouges, blue light and red-blue mixing light irradiation culture 14 under the conditions of oligotrophic
My god, the average dry substance accumulation speed of thallus is 2.35~2.77g/m2/ d, thallus content of starch be 16.19%~
23.19%, and white light culture 14 days is used, the average dry substance accumulation speed of thallus is 2.42g/m2/ d, thallus are formed sediment
Powder content is 15.98%, and the method for the invention can carry on the basis of keeping thallus dry substance accumulation speed to be basically unchanged
High thallus content of starch.
As shown in Figure 10, light irradiation culture 14 days is mixed using feux rouges, blue light and red-blue under the conditions of oligotrophic, it is more
The total starch accumulating rate of root duckweed has reached 3.04~4.18g/m2/ d, and use 14 days more duckweeds of white light culture
Total starch accumulating rate is only 2.5g/m2/ d, illustrate that method provided by the invention can effectively improve the total starch production of more duckweeds
Amount.
Claims (10)
- It is 1. a kind of by promoting hypopus to form the method for improving duckweed total starch yield, it is characterised in that duckweed is inoculated in water In body, cultivated 14~30 days under the conditions of the irradiation of feux rouges, blue light or red-blue mixed light, harvest duckweed hypopus and lobate Body.
- 2. according to claim 1 by promoting hypopus to form the method for improving duckweed total starch yield, it is characterised in that Photon hypothesis according to feux rouges, blue light or red-blue mixed light is 80~200 μm of ol/m2/ s illumination condition culture Duckweed.
- 3. according to claim 1 or claim 2 by promoting hypopus to form the method for improving duckweed total starch yield, its feature exists It is 660nm in the peak wavelength of feux rouges, the peak wavelength of blue light is 450nm.
- 4. according to claim 3 by promoting hypopus to form the method for improving duckweed total starch yield, it is characterised in that The peak width at half height of feux rouges is no more than 15nm, and the peak width at half height of blue light is no more than 20nm.
- 5. according to claim 1 or claim 2 by promoting hypopus to form the method for improving duckweed total starch yield, its feature exists In when mixing light irradiation using red-blue, the ratio between photon hypothesis of feux rouges and blue light in red-blue mixed light is 1: (0.25~1).
- 6. according to claim 3 by promoting hypopus to form the method for improving duckweed total starch yield, it is characterised in that When mixing light irradiation using red-blue, the ratio between photon hypothesis of feux rouges and blue light in red-blue mixed light is 1:(0.25 ~1).
- 7. according to claim 4 by promoting hypopus to form the method for improving duckweed total starch yield, it is characterised in that When mixing light irradiation using red-blue, the ratio between photon hypothesis of feux rouges and blue light in red-blue mixed light is 1:(0.25 ~1).
- 8. according to claim 1 or claim 2 by promoting hypopus to form the method for improving duckweed total starch yield, its feature exists In providing feux rouges using red LED lamp, blue light is provided using blue LED lamp, red LED lamp is combined with blue LED lamp and provided Red-blue mixed light.
- 9. according to claim 1 or claim 2 by promoting hypopus to form the method for improving duckweed total starch yield, its feature exists In the coverage rate according to 80%~100% by duckweed kind in water body.
- It is 10. according to claim 1 or claim 2 by promoting hypopus to form the method for improving duckweed total starch yield, its feature It is more duckweeds to be the duckweed.
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| CN112794920A (en) * | 2021-01-15 | 2021-05-14 | 江南大学 | Method for extracting duckweed dormant body starch |
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